LOCUS       NM_016316               4731 bp    mRNA    linear   PRI 01-OCT-2024
DEFINITION  Homo sapiens REV1 DNA directed polymerase (REV1), transcript
            variant 1, mRNA.
ACCESSION   NM_016316
VERSION     NM_016316.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Zhu,N., Ding,Y., Mi,M., Yang,J., Yang,M., Li,D., Zhang,Y., Fang,X.,
            Weng,S. and Yuan,Y.
  TITLE     Loss-of-function mutation of REV1 (p.R704Q) mediates cetuximab
            primary resistance by activating autophagy in RAS-wild type
            metastatic colorectal cancer
  JOURNAL   Cancer Lett 598, 217103 (2024)
   PUBMED   38969162
  REMARK    GeneRIF: Loss-of-function mutation of REV1 (p.R704Q) mediates
            cetuximab primary resistance by activating autophagy in RAS-wild
            type metastatic colorectal cancer.
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Stolyarenko,A.D., Novikova,A.A., Shilkin,E.S., Poltorachenko,V.A.
            and Makarova,A.V.
  TITLE     The Catalytic Activity of Human REV1 on Undamaged and Damaged DNA
  JOURNAL   Int J Mol Sci 25 (7), 4107 (2024)
   PUBMED   38612916
  REMARK    GeneRIF: The Catalytic Activity of Human REV1 on Undamaged and
            Damaged DNA.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4731)
  AUTHORS   Chen,Y., Jie,X., Xing,B., Wu,Z., Yang,X., Rao,X., Xu,Y., Zhou,D.,
            Dong,X., Zhang,T., Yang,K., Li,Z. and Wu,G.
  TITLE     REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2
            axis
  JOURNAL   Cell Death Dis 13 (2), 110 (2022)
   PUBMED   35115490
  REMARK    GeneRIF: REV1 promotes lung tumorigenesis by activating the
            Rad18/SERTAD2 axis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4731)
  AUTHORS   Bi,T., Niu,X., Qin,C. and Xiao,W.
  TITLE     Genetic and physical interactions between Poleta and Rev1 in
            response to UV-induced DNA damage in mammalian cells
  JOURNAL   Sci Rep 11 (1), 21364 (2021)
   PUBMED   34725419
  REMARK    GeneRIF: Genetic and physical interactions between Poleta and Rev1
            in response to UV-induced DNA damage in mammalian cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4731)
  AUTHORS   Taglialatela,A., Leuzzi,G., Sannino,V., Cuella-Martin,R.,
            Huang,J.W., Wu-Baer,F., Baer,R., Costanzo,V. and Ciccia,A.
  TITLE     REV1-Polzeta maintains the viability of homologous
            recombination-deficient cancer cells through mutagenic repair of
            PRIMPOL-dependent ssDNA gaps
  JOURNAL   Mol Cell 81 (19), 4008-4025 (2021)
   PUBMED   34508659
  REMARK    GeneRIF: REV1-Polzeta maintains the viability of homologous
            recombination-deficient cancer cells through mutagenic repair of
            PRIMPOL-dependent ssDNA gaps.
REFERENCE   6  (bases 1 to 4731)
  AUTHORS   Masuda,Y., Takahashi,M., Tsunekuni,N., Minami,T., Sumii,M.,
            Miyagawa,K. and Kamiya,K.
  TITLE     Deoxycytidyl transferase activity of the human REV1 protein is
            closely associated with the conserved polymerase domain
  JOURNAL   J Biol Chem 276 (18), 15051-15058 (2001)
   PUBMED   11278384
REFERENCE   7  (bases 1 to 4731)
  AUTHORS   Wixler,V., Geerts,D., Laplantine,E., Westhoff,D., Smyth,N.,
            Aumailley,M., Sonnenberg,A. and Paulsson,M.
  TITLE     The LIM-only protein DRAL/FHL2 binds to the cytoplasmic domain of
            several alpha and beta integrin chains and is recruited to adhesion
            complexes
  JOURNAL   J Biol Chem 275 (43), 33669-33678 (2000)
   PUBMED   10906324
REFERENCE   8  (bases 1 to 4731)
  AUTHORS   Gibbs,P.E., Wang,X.D., Li,Z., McManus,T.P., McGregor,W.G.,
            Lawrence,C.W. and Maher,V.M.
  TITLE     The function of the human homolog of Saccharomyces cerevisiae REV1
            is required for mutagenesis induced by UV light
  JOURNAL   Proc Natl Acad Sci U S A 97 (8), 4186-4191 (2000)
   PUBMED   10760286
REFERENCE   9  (bases 1 to 4731)
  AUTHORS   Lin,W., Xin,H., Zhang,Y., Wu,X., Yuan,F. and Wang,Z.
  TITLE     The human REV1 gene codes for a DNA template-dependent dCMP
            transferase
  JOURNAL   Nucleic Acids Res 27 (22), 4468-4475 (1999)
   PUBMED   10536157
REFERENCE   10 (bases 1 to 4731)
  AUTHORS   Wixler,V., Laplantine,E., Geerts,D., Sonnenberg,A., Petersohn,D.,
            Eckes,B., Paulsson,M. and Aumailley,M.
  TITLE     Identification of novel interaction partners for the conserved
            membrane proximal region of alpha-integrin cytoplasmic domains
  JOURNAL   FEBS Lett 445 (2-3), 351-355 (1999)
   PUBMED   10094488
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY010913.1, AF151538.1,
            AC018690.5 and BM683065.1.
            
            On Nov 22, 2018 this sequence version replaced NM_016316.3.
            
            Summary: This gene encodes a protein with similarity to the S.
            cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a
            BRCT domain, which is important in protein-protein interactions. A
            suggested role for the human Rev1-like protein is as a scaffold
            that recruits DNA polymerases involved in translesion synthesis
            (TLS) of damaged DNA. [provided by RefSeq, Mar 2016].
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.2340.1,
                                           SRR1803614.166936.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000258428.8/ ENSP00000258428.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 HY010913.1         41-48
            9-3450              AF151538.1         1-3442
            3451-3451           AC018690.5         32840-32840         c
            3452-4263           AF151538.1         3444-4255
            4264-4731           BM683065.1         20-487              c
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q11.2"
     gene            1..4731
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /note="REV1 DNA directed polymerase"
                     /db_xref="GeneID:51455"
                     /db_xref="HGNC:HGNC:14060"
                     /db_xref="MIM:606134"
     exon            1..201
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            202..265
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     CDS             212..3967
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /note="isoform 1 is encoded by transcript variant 1; REV1-
                     like; DNA repair protein REV1; alpha integrin-binding
                     protein 80; rev1-like terminal deoxycytidyl transferase;
                     REV1 homolog; REV1, polymerase (DNA directed)"
                     /codon_start=1
                     /product="DNA repair protein REV1 isoform 1"
                     /protein_id="NP_057400.1"
                     /db_xref="CCDS:CCDS2045.1"
                     /db_xref="GeneID:51455"
                     /db_xref="HGNC:HGNC:14060"
                     /db_xref="MIM:606134"
                     /translation="MRRGGWRKRAENDGWETWGGYMAAKVQKLEEQFRSDAAMQKDGT
                     SSTIFSGVAIYVNGYTDPSAEELRKLMMLHGGQYHVYYSRSKTTHIIATNLPNAKIKE
                     LKGEKVIRPEWIVESIKAGRLLSYIPYQLYTKQSSVQKGLSFNPVCRPEDPLPGPSNI
                     AKQLNNRVNHIVKKIETENEVKVNGMNSWNEEDENNDFSFVDLEQTSPGRKQNGIPHP
                     RGSTAIFNGHTPSSNGALKTQDCLVPMVNSVASRLSPAFSQEEDKAEKSSTDFRDCTL
                     QQLQQSTRNTDALRNPHRTNSFSLSPLHSNTKINGAHHSTVQGPSSTKSTSSVSTFSK
                     AAPSVPSKPSDCNFISNFYSHSRLHHISMWKCELTEFVNTLQRQSNGIFPGREKLKKM
                     KTGRSALVVTDTGDMSVLNSPRHQSCIMHVDMDCFFVSVGIRNRPDLKGKPVAVTSNR
                     GTGRAPLRPGANPQLEWQYYQNKILKGKAADIPDSSLWENPDSAQANGIDSVLSRAEI
                     ASCSYEARQLGIKNGMFFGHAKQLCPNLQAVPYDFHAYKEVAQTLYETLASYTHNIEA
                     VSCDEALVDITEILAETKLTPDEFANAVRMEIKDQTKCAASVGIGSNILLARMATRKA
                     KPDGQYHLKPEEVDDFIRGQLVTNLPGVGHSMESKLASLGIKTCGDLQYMTMAKLQKE
                     FGPKTGQMLYRFCRGLDDRPVRTEKERKSVSAEINYGIRFTQPKEAEAFLLSLSEEIQ
                     RRLEATGMKGKRLTLKIMVRKPGAPVETAKFGGHGICDNIARTVTLDQATDNAKIIGK
                     AMLNMFHTMKLNISDMRGVGIHVNQLVPTNLNPSTCPSRPSVQSSHFPSGSYSVRDVF
                     QVQKAKKSTEEEHKEVFRAAVDLEISSASRTCTFLPPFPAHLPTSPDTNKAESSGKWN
                     GLHTPVSVQSRLNLSIEVPSPSQLDQSVLEALPPDLREQVEQVCAVQQAESHGDKKKE
                     PVNGCNTGILPQPVGTVLLQIPEPQESNSDAGINLIALPAFSQVDPEVFAALPAELQR
                     ELKAAYDQRQRQGENSTHQQSASASVPKNPLLHLKAAVKEKKRNKKKKTIGSPKRIQS
                     PLNNKLLNSPAKTLPGACGSPQKLIDGFLKHEGPPAEKPLEELSASTSGVPGLSSLQS
                     DPAGCVRPPAPNLAGAVEFNDVKTLLREWITTISDPMEEDILQVVKYCTDLIEEKDLE
                     KLDLVIKYMKRLMQQSVESVWNMAFDFILDNVQVVLQQTYGSTLKVT"
     exon            266..392
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            393..561
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            562..714
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            715..1424
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1425..1532
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1533..1649
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1758
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1759..1887
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1888..2042
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            2043..2162
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            2163..2383
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            2384..2556
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            2557..2659
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            2660..2825
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            2826..3022
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            3023..3256
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            3257..3377
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            3378..3595
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            3596..3752
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            3753..3855
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     exon            3856..4731
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4241..4246
                     /regulatory_class="polyA_signal_sequence"
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /note="hexamer: AATAAA"
     polyA_site      4258
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /note="major polyA site"
     regulatory      4711..4716
                     /regulatory_class="polyA_signal_sequence"
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /note="hexamer: AATAAA"
     polyA_site      4731
                     /gene="REV1"
                     /gene_synonym="AIBP80; REV1L"
                     /note="major polyA site"
ORIGIN      
        1 gagccaccgc ggagcgcgcg cggggttggt tgccgcgagc gtgggggagc gtggaccgcg
       61 gcgctgctca gcggtggggc tgccttcccc cggccctcct ccctggtccc tggcgagggc
      121 actggcggcg gcggggccgg ggtccgcaag gccggagaag gccgccgggc ccgggcatgg
      181 tggtctgggg caacgcggaa gaagctccac catgaggcga ggtggatgga ggaagcgagc
      241 tgaaaatgat ggctgggaaa catggggtgg gtatatggct gccaaggtcc agaaattgga
      301 ggaacagttt cgatcagatg ctgctatgca gaaggatggg acttcatcta caatttttag
      361 tggagttgcc atctatgtta atggatacac agatccttcc gctgaggaat tgagaaaact
      421 aatgatgttg catggaggtc aataccatgt atattattcc agatctaaaa caacacatat
      481 tattgccaca aatcttccca atgccaaaat taaagaatta aagggggaaa aagtaattcg
      541 accagaatgg attgtggaaa gcatcaaagc tggacgactc ctctcctaca ttccatatca
      601 gctgtacacc aagcagtcca gtgtgcagaa aggtctcagc tttaatcctg tatgcagacc
      661 tgaggatcct ctgccaggtc caagcaatat agccaaacag ctcaacaaca gggtaaatca
      721 catcgttaag aagattgaaa cggaaaatga agtcaaagtc aatggcatga acagttggaa
      781 tgaagaagat gaaaataatg attttagttt tgtggatctg gagcagacct ctccgggaag
      841 gaaacagaat ggaattccgc atcccagagg gagcactgcc atttttaatg gacacactcc
      901 tagctctaat ggtgccttaa agacacagga ttgcttggtg cccatggtca acagtgttgc
      961 cagcaggctt tctccagcct tttcccagga ggaggataag gctgagaaga gcagcactga
     1021 tttcagagac tgcactctgc agcagttgca gcaaagcacc agaaacacag atgctttgcg
     1081 gaatccacac agaactaatt ctttctcatt atcacctttg cacagtaaca ctaaaatcaa
     1141 tggtgctcac cactccactg ttcaggggcc ttcaagcaca aaaagcactt cttcagtatc
     1201 tacgtttagc aaggcagcac cttcagtgcc atccaaacct tcagactgca attttatttc
     1261 aaacttctat tctcattcaa gactgcatca catatcaatg tggaagtgtg aattgactga
     1321 gtttgtcaat accctacaaa gacaaagtaa tggtatcttt ccaggaaggg aaaagttaaa
     1381 aaaaatgaaa acaggcaggt ctgcacttgt tgtaactgac acaggagata tgtcagtatt
     1441 gaattctccc agacatcaga gctgtataat gcatgttgat atggattgct tctttgtatc
     1501 agtgggtata cgaaatagac cagatctcaa aggaaaacca gtggctgtta caagtaacag
     1561 aggcacagga agggcacctt tacgtcctgg cgctaacccc cagctggagt ggcagtatta
     1621 ccagaataaa atcctgaaag gcaaagcagc agatatacca gattcatcat tgtgggagaa
     1681 tccagattct gcgcaagcaa atggaattga ttctgttttg tcaagggctg aaattgcatc
     1741 ttgtagttat gaggccaggc aacttggcat taagaacgga atgttttttg ggcatgctaa
     1801 acaactatgt cctaatcttc aagctgttcc atacgatttt catgcatata aggaagtcgc
     1861 acaaacattg tatgaaacat tggcaagcta cactcataac attgaagctg tcagttgtga
     1921 tgaagcgctg gtagacatta ccgaaatcct tgcagagacc aaacttactc ctgatgaatt
     1981 tgcaaatgct gttcgtatgg aaatcaaaga ccagacgaaa tgtgctgcct ctgttggaat
     2041 tggttctaat attctcctgg ctagaatggc aactagaaaa gcaaaaccag atgggcagta
     2101 ccacctaaaa ccagaagaag tagatgattt tatcagaggc cagctagtga ccaatctacc
     2161 aggagttgga cattcaatgg aatctaagtt ggcatctttg ggaattaaaa cttgtggaga
     2221 cttgcagtat atgaccatgg caaaactcca aaaagaattt ggtcccaaaa caggtcagat
     2281 gctttatagg ttctgccgtg gcttggatga tagaccagtt cgaactgaaa aggaaagaaa
     2341 atctgtttca gctgagatca actatggaat aaggtttact cagccaaaag aggcagaagc
     2401 ttttcttctg agtctttcag aagaaattca aagaagacta gaagccactg gcatgaaggg
     2461 taaacgtcta actctcaaaa tcatggtacg aaagcctggg gctcctgtag aaactgcaaa
     2521 atttggaggc catggaattt gtgataacat tgccaggact gtaactcttg accaggcaac
     2581 agataatgca aaaataattg gaaaggcgat gctaaacatg tttcatacaa tgaaactaaa
     2641 tatatcagat atgagagggg ttgggattca cgtgaatcag ttggttccaa ctaatctgaa
     2701 cccttccaca tgtcccagtc gcccatcagt tcagtcaagc cactttccta gtgggtcata
     2761 ctctgtccgt gatgtcttcc aagttcagaa agctaagaaa tccaccgaag aggagcacaa
     2821 agaagtattt cgggctgctg tggatctgga aatatcatct gcttctagaa cttgcacttt
     2881 cttgccacct tttcctgcac atctgccgac cagtcctgat actaacaagg ctgagtcttc
     2941 agggaaatgg aatggtctac atactcctgt cagtgtgcag tcgagactta acctgagtat
     3001 agaggtcccg tcaccttccc agctggatca gtctgtttta gaagcacttc cacctgatct
     3061 ccgggaacaa gtagagcaag tctgtgctgt ccagcaagca gagtcacatg gcgacaaaaa
     3121 gaaagaacca gtaaatggct gtaatacagg aattttgcca caaccagttg ggacagtctt
     3181 gttgcaaata ccagaacctc aagaatcgaa cagtgacgca ggaataaatt taatagccct
     3241 tccagcattt tcacaggtgg accctgaggt atttgctgcc cttcctgctg aacttcagag
     3301 ggagctgaaa gcagcgtatg atcaaagaca aaggcagggc gagaacagca ctcaccagca
     3361 gtcagccagc gcatctgtgc caaagaatcc tttacttcat ctaaaggcag cagtgaaaga
     3421 aaagaaaaga aacaagaaga aaaaaaccat tggttcacca aaaaggattc agagtccttt
     3481 gaataacaag ctgcttaaca gtcctgcaaa aactctgcca ggggcctgtg gcagtcccca
     3541 gaagttaatt gatgggtttc taaaacatga aggacctcct gcagagaaac ccctggaaga
     3601 actctctgct tctacttcag gtgtgccagg cctttctagt ttgcagtctg acccagctgg
     3661 ctgtgtgaga cctccagcac ccaatctagc tggagctgtt gaattcaatg atgtgaagac
     3721 cttgctcaga gaatggataa ctacaatttc agatccaatg gaagaagaca ttctccaagt
     3781 tgtgaaatac tgtactgatc taatagaaga aaaagatttg gaaaaactgg atctagttat
     3841 aaaatacatg aaaaggctga tgcagcaatc ggtggaatcg gtttggaata tggcatttga
     3901 ctttattctt gacaatgtcc aggtggtttt acaacaaact tatggaagca cattaaaagt
     3961 tacataaata ttaccagaga gcctgatgct ctctgatagc tgtgccataa gtgcttgtga
     4021 ggtatttgca aagtgcatga tagtaatgct cggagttttt ataattttaa atttctttta
     4081 aagcaagtgt tttgtacatt tcttttcaaa aagtgccaaa tttgtcagta ttgcatgtaa
     4141 ataattgtgt taattatttt actgtagcat agattctatt tacaaaatgt ttgtttataa
     4201 agttttatgg atttttacag tgaagtgttt acagttgttt aataaagaac tgtatgtata
     4261 ttttgtacag gctccttttt gtgaatcctt aaaaactcaa ctctaggaag caactactgt
     4321 ttattatact aaaaggctga aaaacctcca ggccagactg ctaagctctg aaattcctga
     4381 gaggtctcag accgggattc tacttgttcc aagaaagggt aaagcttcta aaccatctta
     4441 ttcttgtctc caagcatgaa cacaggagca tgttaagaaa atctttacta cttcttccat
     4501 gcggagaaat ctacatattt tgaattagaa acaccctcac acccacttga agattttttt
     4561 cctgggaaca ttatgtcccg tagatcagag gtggtgttgt ctttttgctt ctactggcca
     4621 ttgagaaact ttgatgataa aaaagaacgg tatagatttt tcaaacgtat ataaaatatt
     4681 tttatgttat atgttatgcc ataactttaa aataaaaata gtttaaaatt c
//
