LOCUS       NM_017750               3141 bp    mRNA    linear   PRI 06-AUG-2024
DEFINITION  Homo sapiens retinol saturase (RETSAT), mRNA.
ACCESSION   NM_017750
VERSION     NM_017750.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Kiefer,M.F., Meng,Y., Yang,N., Vahrenbrink,M., Wulff,S., Li,C.,
            Wowro,S.J., Petricek,K.M., Sommerfeld,M., Flores,R.E.,
            Obermayer,B., Piepelow,K., Klaus,S., Hartl,K., Guillot,A.,
            Tacke,F., Sigal,M. and Schupp,M.
  TITLE     Intestinal retinol saturase is implicated in the development of
            obesity and epithelial homeostasis upon injury
  JOURNAL   Am J Physiol Endocrinol Metab 327 (2), E203-E216 (2024)
   PUBMED   38895981
  REMARK    GeneRIF: Intestinal retinol saturase is implicated in the
            development of obesity and epithelial homeostasis upon injury.
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Tu,Q., Liu,X., Yao,X., Li,R., Liu,G., Jiang,H., Li,K., Chen,Q.,
            Huang,X., Chang,Q., Xu,G., Zhu,H., Shi,P. and Zhao,B.
  TITLE     RETSAT associates with DDX39B to promote fork restarting and
            resistance to gemcitabine based chemotherapy in pancreatic ductal
            adenocarcinoma
  JOURNAL   J Exp Clin Cancer Res 41 (1), 274 (2022)
   PUBMED   36109793
  REMARK    GeneRIF: RETSAT associates with DDX39B to promote fork restarting
            and resistance to gemcitabine based chemotherapy in pancreatic
            ductal adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3141)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   4  (bases 1 to 3141)
  AUTHORS   Schupp,M., Lefterova,M.I., Janke,J., Leitner,K., Cristancho,A.G.,
            Mullican,S.E., Qatanani,M., Szwergold,N., Steger,D.J., Curtin,J.C.,
            Kim,R.J., Suh,M.J., Albert,M.R., Engeli,S., Gudas,L.J. and
            Lazar,M.A.
  TITLE     Retinol saturase promotes adipogenesis and is downregulated in
            obesity
  JOURNAL   Proc Natl Acad Sci U S A 106 (4), 1105-1110 (2009)
   PUBMED   19139408
  REMARK    GeneRIF: RetSat plays an important role in the biology of
            adipocytes, where it favors normal differentiation, yet is reduced
            in the obese state.
            Erratum:[Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4571. Suh,
            Moojin [corrected to Suh, Moo-Jin]]
REFERENCE   5  (bases 1 to 3141)
  AUTHORS   Moise,A.R., Kuksa,V., Imanishi,Y. and Palczewski,K.
  TITLE     Identification of all-trans-retinol:all-trans-13,14-dihydroretinol
            saturase
  JOURNAL   J Biol Chem 279 (48), 50230-50242 (2004)
   PUBMED   15358783
REFERENCE   6  (bases 1 to 3141)
  AUTHORS   Clark,H.F., Gurney,A.L., Abaya,E., Baker,K., Baldwin,D., Brush,J.,
            Chen,J., Chow,B., Chui,C., Crowley,C., Currell,B., Deuel,B.,
            Dowd,P., Eaton,D., Foster,J., Grimaldi,C., Gu,Q., Hass,P.E.,
            Heldens,S., Huang,A., Kim,H.S., Klimowski,L., Jin,Y., Johnson,S.,
            Lee,J., Lewis,L., Liao,D., Mark,M., Robbie,E., Sanchez,C.,
            Schoenfeld,J., Seshagiri,S., Simmons,L., Singh,J., Smith,V.,
            Stinson,J., Vagts,A., Vandlen,R., Watanabe,C., Wieand,D., Woods,K.,
            Xie,M.H., Yansura,D., Yi,S., Yu,G., Yuan,J., Zhang,M., Zhang,Z.,
            Goddard,A., Wood,W.I., Godowski,P. and Gray,A.
  TITLE     The secreted protein discovery initiative (SPDI), a large-scale
            effort to identify novel human secreted and transmembrane proteins:
            a bioinformatics assessment
  JOURNAL   Genome Res 13 (10), 2265-2270 (2003)
   PUBMED   12975309
  REMARK    Erratum:[Genome Res. 2003 Dec;13(12):2759]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK075261.1, DB001412.1, BC068517.1 and BQ448420.1.
            
            On Nov 23, 2018 this sequence version replaced NM_017750.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC068517.1, SRR1803617.125033.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000295802.9/ ENSP00000295802.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1078              AK075261.1         5-1082
            1079-1616           DB001412.1         2-539
            1617-2990           BC068517.1         1617-2990
            2991-3141           BQ448420.1         18-168              c
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p11.2"
     gene            1..3141
                     /gene="RETSAT"
                     /note="retinol saturase"
                     /db_xref="GeneID:54884"
                     /db_xref="HGNC:HGNC:25991"
                     /db_xref="MIM:617597"
     exon            1..193
                     /gene="RETSAT"
                     /inference="alignment:Splign:2.1.0"
     CDS             22..1854
                     /gene="RETSAT"
                     /EC_number="1.3.99.23"
                     /note="all-trans-13,14-dihydroretinol saturase;
                     all-trans-retinol 13,14-reductase; PPAR-alpha-regulated
                     and starvation-induced gene protein"
                     /codon_start=1
                     /product="all-trans-retinol 13,14-reductase precursor"
                     /protein_id="NP_060220.3"
                     /db_xref="CCDS:CCDS1972.1"
                     /db_xref="GeneID:54884"
                     /db_xref="HGNC:HGNC:25991"
                     /db_xref="MIM:617597"
                     /translation="MWLPLVLLLAVLLLAVLCKVYLGLFSGSSPNPFSEDVKRPPAPL
                     VTDKEARKKVLKQAFSANQVPEKLDVVVIGSGFGGLAAAAILAKAGKRVLVLEQHTKA
                     GGCCHTFGKNGLEFDTGIHYIGRMEEGSIGRFILDQITEGQLDWAPLSSPFDIMVLEG
                     PNGRKEYPMYSGEKAYIQGLKEKFPQEEAIIDKYIKLVKVVSSGAPHAILLKFLPLPV
                     VQLLDRCGLLTRFSPFLQASTQSLAEVLQQLGASSELQAVLSYIFPTYGVTPNHSAFS
                     MHALLVNHYMKGGFYPRGGSSEIAFHTIPVIQRAGGAVLTKATVQSVLLDSAGKACGV
                     SVKKGHELVNIYCPIVVSNAGLFNTYEHLLPGNARCLPGVKQQLGTVRPGLGMTSVFI
                     CLRGTKEDLHLPSTNYYVYYDTDMDQAMERYVSMPREEAAEHIPLLFFAFPSAKDPTW
                     EDRFPGRSTMIMLIPTAYEWFEEWQAELKGKRGSDYETFKNSFVEASMSVVLKLFPQL
                     EGKVESVTAGSPLTNQFYLAAPRGACYGADHDLGRLHPCVMASLRAQSPIPNLYLTGQ
                     DIFTCGLVGALQGALLCSSAILKRNLYSDLKNLDSRIRAQKKKN"
     exon            194..376
                     /gene="RETSAT"
                     /inference="alignment:Splign:2.1.0"
     exon            377..618
                     /gene="RETSAT"
                     /inference="alignment:Splign:2.1.0"
     exon            619..820
                     /gene="RETSAT"
                     /inference="alignment:Splign:2.1.0"
     exon            821..1018
                     /gene="RETSAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..1138
                     /gene="RETSAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1139..1277
                     /gene="RETSAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1387
                     /gene="RETSAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..1554
                     /gene="RETSAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1555..1714
                     /gene="RETSAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1715..3141
                     /gene="RETSAT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2972..2977
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RETSAT"
                     /note="hexamer: AATAAA"
     polyA_site      2999
                     /gene="RETSAT"
                     /note="major polyA site"
     regulatory      3071..3076
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RETSAT"
                     /note="hexamer: AGTAAA"
     polyA_site      3102
                     /gene="RETSAT"
     regulatory      3116..3121
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RETSAT"
                     /note="hexamer: AATAAA"
     polyA_site      3141
                     /gene="RETSAT"
ORIGIN      
        1 acccgacccg acgccggcgt gatgtggctt ccgctggtgc tgctcctggc tgtgctgctg
       61 ctggccgtcc tctgcaaagt ttacttggga ctattctctg gcagctcccc gaatcctttc
      121 tccgaagatg tcaaacggcc cccagcgccc ctggtaactg acaaggaggc caggaagaag
      181 gttctcaaac aagctttttc agccaaccaa gtgccggaga agctggatgt ggtggtaatt
      241 ggcagtggct ttgggggcct ggctgcagct gcaattctag ctaaagctgg caagcgagtc
      301 ctggtgctgg aacaacatac caaggcaggg ggctgctgtc atacctttgg aaagaatggc
      361 cttgaatttg acacaggaat ccattacatt gggcgtatgg aagagggcag cattggccgt
      421 tttatcttgg accagatcac tgaagggcag ctggactggg ctcccctgtc ctctcctttt
      481 gacatcatgg tactggaagg gcccaatggc cgaaaggagt accccatgta cagtggagag
      541 aaagcctaca ttcagggcct caaggagaag tttccacagg aggaagctat cattgacaag
      601 tatataaagc tggttaaggt ggtatccagt ggagcccctc atgccatcct gttgaaattc
      661 ctcccattgc ccgtggttca gctcctcgac aggtgtgggc tgctgactcg tttctctcca
      721 ttccttcaag catccaccca gagcctggct gaggtcctgc agcagctggg ggcctcctct
      781 gagctccagg cagtactcag ctacatcttc cccacttacg gtgtcacccc caaccacagt
      841 gccttttcca tgcacgccct gctggtcaac cactacatga aaggaggctt ttatccccga
      901 gggggttcca gtgaaattgc cttccacacc atccctgtga ttcagcgggc tgggggcgct
      961 gtcctcacaa aggccactgt gcagagtgtg ttgctggact cagctgggaa agcctgtggt
     1021 gtcagtgtga agaaggggca tgagctggtg aacatctatt gccccatcgt ggtctccaac
     1081 gcaggactgt tcaacaccta tgaacaccta ctgccgggga acgcccgctg cctgccaggt
     1141 gtgaagcagc aactggggac ggtgcggccc ggcttaggca tgacctctgt tttcatctgc
     1201 ctgcgaggca ccaaggaaga cctgcatctg ccgtccacca actactatgt ttactatgac
     1261 acggacatgg accaggcgat ggagcgctac gtctccatgc ccagggaaga ggctgcggaa
     1321 cacatccctc ttctcttctt cgctttccca tcagccaaag atccgacctg ggaggaccga
     1381 ttcccaggcc ggtccaccat gatcatgctc atacccactg cctacgagtg gtttgaggag
     1441 tggcaggcgg agctgaaggg aaagcggggc agtgactatg agaccttcaa aaactccttt
     1501 gtggaagcct ctatgtcagt ggtcctgaaa ctgttcccac agctggaggg gaaggtggag
     1561 agtgtgactg caggatcccc actcaccaac cagttctatc tggctgctcc ccgaggtgcc
     1621 tgctacgggg ctgaccatga cctgggccgc ctgcaccctt gtgtgatggc ctccttgagg
     1681 gcccagagcc ccatccccaa cctctatctg acaggccagg atatcttcac ctgtggactg
     1741 gtcggggccc tgcaaggtgc cctgctgtgc agcagcgcca tcctgaagcg gaacttgtac
     1801 tcagacctta agaatcttga ttctaggatc cgggcacaga agaaaaagaa ttagttccat
     1861 cagggaggag tcagaggaat ttgcccaatg gctggggcat ctcccttgac ttacccataa
     1921 tgtctttctg cattagttcc ttgcacgtat aaagcactct aatttggttc tgatgcctga
     1981 agagaggcct agtttaaatc acaattccga atctggggca atggaatcac tgcttccagc
     2041 tggggcaggt gagatcttta cgccttttat aacatgccat ccctactaat aggatattga
     2101 cttggatagc ttgatgtctc atgacgagcg gcgctctgca tccctcaccc atgcctccta
     2161 actcagtgat caaagcgaat attccatctg tggatagaac ccctggcagt gttgtcagct
     2221 caacctggtg ggttcagttc tgtcctgagg cttctgctct cattcattta gtgctacgct
     2281 gcacagttct acactgtcaa gggaaaaggg agactaatga ggcttaactc aaaacctggg
     2341 cgtggttttg gttgccattc cataggtttg gagagctcta gatctctttt gtgctgggtt
     2401 cagtggctct tcaggggaca ggaaatgcct gtgtctggcc agtgtggttc tggagctttg
     2461 gggtaacagc aggatccatc agttagtagg gtgcatgtca gatgatcata tccaattcat
     2521 atggaagtcc cgggtctgtc ttccttatca tcggggtggc agctggttct caatgtgcca
     2581 gcagggactc agtacctgag cctcaatcaa gccttatcca ccaaatacac agggaagggt
     2641 gatgcaggga agggtgacat caggagtcag ggcatggact ggtaagatga atactttgct
     2701 gggctgaagc aggctgcagg gcattccagc caagggcaca gcaggggaca gtgcagggag
     2761 gtgtggggta agggagggaa gtcacatcag aaaagggaaa gccacggaat gtgtgtgaag
     2821 cccagaaatg gcatttgcag ttaattagca catgtgaggg ttagacaggt aggtgaatgc
     2881 aagctcaagg tttggaaaaa tgacttttca gttatgtctt tggtatcaga catacgaaag
     2941 gtctctttgt agttcgtgtt aatgtaacat taataaattt attgattcca ttgctttaac
     3001 atttgaaatt tattttggtt ttttgttcaa gaaaacaaaa ctattattgt gatggcattt
     3061 gcagaagctc agtaaaacac tatatactga ataacaccaa aataagcttt aaaaaaataa
     3121 aattaagtaa ttataaagtt a
//
