LOCUS       NM_032044               1224 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens regenerating family member 4 (REG4), transcript
            variant 2, mRNA.
ACCESSION   NM_032044
VERSION     NM_032044.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Zhang,C.Y., Zhang,R., Zhang,L., Wang,Z.M., Sun,H.Z., Cui,Z.G. and
            Zheng,H.C.
  TITLE     Regenerating gene 4 promotes chemoresistance of colorectal cancer
            by affecting lipid droplet synthesis and assembly
  JOURNAL   World J Gastroenterol 29 (35), 5104-5124 (2023)
   PUBMED   37744296
  REMARK    GeneRIF: Regenerating gene 4 promotes chemoresistance of colorectal
            cancer by affecting lipid droplet synthesis and assembly.
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Xiang,L.W., Xue,H., Ha,M.W., Yu,D.Y., Xiao,L.J. and Zheng,H.C.
  TITLE     The effects of REG4 expression on chemoresistance of ovarian cancer
  JOURNAL   J Obstet Gynaecol 42 (7), 3149-3157 (2022)
   PUBMED   35929918
  REMARK    GeneRIF: The effects of REG4 expression on chemoresistance of
            ovarian cancer.
REFERENCE   3  (bases 1 to 1224)
  AUTHORS   Takasawa,S., Tsuchida,C., Sakuramoto-Tsuchida,S., Uchiyama,T.,
            Makino,M., Yamauchi,A. and Itaya-Hironaka,A.
  TITLE     Upregulation of REG IV gene in human intestinal epithelial cells by
            lipopolysaccharide via downregulation of microRNA-24
  JOURNAL   J Cell Mol Med 26 (17), 4710-4720 (2022)
   PUBMED   35946046
  REMARK    GeneRIF: Upregulation of REG IV gene in human intestinal epithelial
            cells by lipopolysaccharide via downregulation of microRNA-24.
REFERENCE   4  (bases 1 to 1224)
  AUTHORS   Bishnupuri,K.S., Sainathan,S.K., Ciorba,M.A., Houchen,C.W. and
            Dieckgraefe,B.K.
  TITLE     Reg4 Interacts with CD44 to Regulate Proliferation and Stemness of
            Colorectal and Pancreatic Cancer Cells
  JOURNAL   Mol Cancer Res 20 (3), 387-399 (2022)
   PUBMED   34753802
  REMARK    GeneRIF: Reg4 Interacts with CD44 to Regulate Proliferation and
            Stemness of Colorectal and Pancreatic Cancer Cells.
REFERENCE   5  (bases 1 to 1224)
  AUTHORS   Kang,G., Oh,I., Pyo,J., Kang,D. and Son,B.
  TITLE     Clinicopathological Significance and Prognostic Implications of
            REG4 Immunohistochemical Expression in Colorectal Cancer
  JOURNAL   Medicina (Kaunas) 57 (9), 938 (2021)
   PUBMED   34577861
  REMARK    GeneRIF: Clinicopathological Significance and Prognostic
            Implications of REG4 Immunohistochemical Expression in Colorectal
            Cancer.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1224)
  AUTHORS   Zhang,Z. and Henzel,W.J.
  TITLE     Signal peptide prediction based on analysis of experimentally
            verified cleavage sites
  JOURNAL   Protein Sci 13 (10), 2819-2824 (2004)
   PUBMED   15340161
REFERENCE   7  (bases 1 to 1224)
  AUTHORS   Zhang,Y., Lai,M., Lv,B., Gu,X., Wang,H., Zhu,Y., Zhu,Y., Shao,L.
            and Wang,G.
  TITLE     Overexpression of Reg IV in colorectal adenoma
  JOURNAL   Cancer Lett 200 (1), 69-76 (2003)
   PUBMED   14550954
  REMARK    GeneRIF: overexpression of Reg IV may be an early event in
            colorectal carcinogenesis.
REFERENCE   8  (bases 1 to 1224)
  AUTHORS   Kamarainen,M., Heiskala,K., Knuutila,S., Heiskala,M., Winqvist,O.
            and Andersson,L.C.
  TITLE     RELP, a novel human REG-like protein with up-regulated expression
            in inflammatory and metaplastic gastrointestinal mucosa
  JOURNAL   Am J Pathol 163 (1), 11-20 (2003)
   PUBMED   12819006
  REMARK    GeneRIF: Results suggest that RELP might be involved in
            inflammatory and metaplastic responses of the gastrointestinal
            epithelium.
REFERENCE   9  (bases 1 to 1224)
  AUTHORS   Violette,S., Festor,E., Pandrea-Vasile,I., Mitchell,V., Adida,C.,
            Dussaulx,E., Lacorte,J.M., Chambaz,J., Lacasa,M. and Lesuffleur,T.
  TITLE     Reg IV, a new member of the regenerating gene family, is
            overexpressed in colorectal carcinomas
  JOURNAL   Int J Cancer 103 (2), 185-193 (2003)
   PUBMED   12455032
REFERENCE   10 (bases 1 to 1224)
  AUTHORS   Hartupee,J.C., Zhang,H., Bonaldo,M.F., Soares,M.B. and
            Dieckgraefe,B.K.
  TITLE     Isolation and characterization of a cDNA encoding a novel member of
            the human regenerating protein family: Reg IV
  JOURNAL   Biochim Biophys Acta 1518 (3), 287-293 (2001)
   PUBMED   11311942
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL359752.11, AY007243.1 and AY126670.1.
            
            On Nov 23, 2018 this sequence version replaced NM_032044.3.
            
            Transcript Variant: This variant (2) lacks an exon in the 5' UTR,
            as compared to variant 1. Variants 1 and 2 encode the same protein
            (isoform 1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF254415.1, BC017089.2 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000256585.10/ ENSP00000256585.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AL359752.11        20966-20989         c
            25-1078             AY007243.1         1-1054
            1079-1223           AY126670.1         1373-1517
            1224-1224           AL359752.11        3546-3546           c
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p12"
     gene            1..1224
                     /gene="REG4"
                     /gene_synonym="GISP; REG-IV; RELP"
                     /note="regenerating family member 4"
                     /db_xref="GeneID:83998"
                     /db_xref="HGNC:HGNC:22977"
                     /db_xref="MIM:609846"
     exon            1..52
                     /gene="REG4"
                     /gene_synonym="GISP; REG-IV; RELP"
                     /inference="alignment:Splign:2.1.0"
     exon            53..213
                     /gene="REG4"
                     /gene_synonym="GISP; REG-IV; RELP"
                     /inference="alignment:Splign:2.1.0"
     CDS             147..623
                     /gene="REG4"
                     /gene_synonym="GISP; REG-IV; RELP"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 2; regenerating gene type IV; gastrointestinal
                     secretory protein; regenerating islet-derived protein 4;
                     REG-4; reg IV; REG-like protein; regenerating
                     islet-derived protein IV; regenerating islet-derived
                     family, member 4"
                     /codon_start=1
                     /product="regenerating islet-derived protein 4 isoform 1
                     precursor"
                     /protein_id="NP_114433.1"
                     /db_xref="CCDS:CCDS906.1"
                     /db_xref="GeneID:83998"
                     /db_xref="HGNC:HGNC:22977"
                     /db_xref="MIM:609846"
                     /translation="MASRSMRLLLLLSCLAKTGVLGDIIMRPSCAPGWFYHKSNCYGY
                     FRKLRNWSDAELECQSYGNGAHLASILSLKEASTIAEYISGYQRSQPIWIGLHDPQKR
                     QQWQWIDGAMYLYRSWSGKSMGGNKHCAEMSSNNNFLTWSSNECNKRQHFLCKYRP"
     exon            214..311
                     /gene="REG4"
                     /gene_synonym="GISP; REG-IV; RELP"
                     /inference="alignment:Splign:2.1.0"
     exon            312..449
                     /gene="REG4"
                     /gene_synonym="GISP; REG-IV; RELP"
                     /inference="alignment:Splign:2.1.0"
     exon            450..555
                     /gene="REG4"
                     /gene_synonym="GISP; REG-IV; RELP"
                     /inference="alignment:Splign:2.1.0"
     exon            556..1224
                     /gene="REG4"
                     /gene_synonym="GISP; REG-IV; RELP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1134..1139
                     /regulatory_class="polyA_signal_sequence"
                     /gene="REG4"
                     /gene_synonym="GISP; REG-IV; RELP"
                     /note="hexamer: AATAAA"
     polyA_site      1154
                     /gene="REG4"
                     /gene_synonym="GISP; REG-IV; RELP"
                     /note="major polyA site"
     regulatory      1201..1206
                     /regulatory_class="polyA_signal_sequence"
                     /gene="REG4"
                     /gene_synonym="GISP; REG-IV; RELP"
                     /note="hexamer: AATAAG"
     polyA_site      1224
                     /gene="REG4"
                     /gene_synonym="GISP; REG-IV; RELP"
ORIGIN      
        1 actggagaca ctgaagaagg caggggccct tagagtcttg gttgccaaac agatttgcag
       61 atcaaggaga acccaggagt ttcaaagaag cgctagtaag gtctctgaga tccttgcact
      121 agctacatcc tcagggtagg aggaagatgg cttccagaag catgcggctg ctcctattgc
      181 tgagctgcct ggccaaaaca ggagtcctgg gtgatatcat catgagaccc agctgtgctc
      241 ctggatggtt ttaccacaag tccaattgct atggttactt caggaagctg aggaactggt
      301 ctgatgccga gctcgagtgt cagtcttacg gaaacggagc ccacctggca tctatcctga
      361 gtttaaagga agccagcacc atagcagagt acataagtgg ctatcagaga agccagccga
      421 tatggattgg cctgcacgac ccacagaaga ggcagcagtg gcagtggatt gatggggcca
      481 tgtatctgta cagatcctgg tctggcaagt ccatgggtgg gaacaagcac tgtgctgaga
      541 tgagctccaa taacaacttt ttaacttgga gcagcaacga atgcaacaag cgccaacact
      601 tcctgtgcaa gtaccgacca tagagcaaga atcaagattc tgctaactcc tgcacagccc
      661 cgtcctcttc ctttctgcta gcctggctaa atctgctcat tatttcagag gggaaaccta
      721 gcaaactaag agtgataagg gccctactac actggctttt ttaggcttag agacagaaac
      781 tttagcattg gcccagtagt ggcttctagc tctaaatgtt tgccccgcca tccctttcca
      841 cagtatcctt cttccctcct cccctgtctc tggctgtctc gagcagtcta gaagagtgca
      901 tctccagcct atgaaacagc tgggtctttg gccataagaa gtaaagattt gaagacagaa
      961 ggaagaaact caggagtaag cttctagacc ccttcagctt ctacaccctt ctgccctctc
     1021 tccattgcct gcaccccacc ccagccactc aactcctgct tgtttttcct ttggccatgg
     1081 gaaggtttac cagtagaatc cttgctaggt tgatgtgggc catacattcc tttaataaac
     1141 cattgtgtac ataagaggtt gctgtgttcc agttcagtaa tggtgaatgt ggaaaagtga
     1201 aataagacca agaaatacac ccaa
//
