LOCUS       NM_003708               1698 bp    mRNA    linear   PRI 02-JUN-2024
DEFINITION  Homo sapiens retinol dehydrogenase 16 (RDH16), transcript variant
            1, mRNA.
ACCESSION   NM_003708
VERSION     NM_003708.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Hu,P.S., Xia,Q.S., Wu,F., Li,D.K., Qi,Y.J., Hu,Y., Wei,Z.Z.,
            Li,S.S., Tian,N.Y., Wei,Q.F., Shen,L.J., Yin,B., Jiang,T.,
            Yuan,J.G., Qiang,B.Q., Han,W. and Peng,X.Z.
  TITLE     NSPc1 promotes cancer stem cell self-renewal by repressing the
            synthesis of all-trans retinoic acid via targeting RDH16 in
            malignant glioma
  JOURNAL   Oncogene 36 (33), 4706-4718 (2017)
   PUBMED   28394339
  REMARK    GeneRIF: RDH16 is a stemness suppressor that partially rescues stem
            cell-like glioma cells from the NSPc1-induced increase in
            neurosphere formation.
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Persson,B., Kallberg,Y., Bray,J.E., Bruford,E., Dellaporta,S.L.,
            Favia,A.D., Duarte,R.G., Jornvall,H., Kavanagh,K.L., Kedishvili,N.,
            Kisiela,M., Maser,E., Mindnich,R., Orchard,S., Penning,T.M.,
            Thornton,J.M., Adamski,J. and Oppermann,U.
  TITLE     The SDR (short-chain dehydrogenase/reductase and related enzymes)
            nomenclature initiative
  JOURNAL   Chem Biol Interact 178 (1-3), 94-98 (2009)
   PUBMED   19027726
REFERENCE   3  (bases 1 to 1698)
  AUTHORS   Lapshina,E.A., Belyaeva,O.V., Chumakova,O.V. and Kedishvili,N.Y.
  TITLE     Differential recognition of the free versus bound retinol by human
            microsomal retinol/sterol dehydrogenases: characterization of the
            holo-CRBP dehydrogenase activity of RoDH-4
  JOURNAL   Biochemistry 42 (3), 776-784 (2003)
   PUBMED   12534290
  REMARK    GeneRIF: Characterization of the retinol dehydrogenase activity of
            RoDH-4 identifies it as the enzyme capable of accessing the bound
            form of retinol for retinoic acid production.
REFERENCE   4  (bases 1 to 1698)
  AUTHORS   Cain,J.M., Zaino,R., Shearer,D., Bennett,R.A., Olt,G. and Weisz,J.
  TITLE     Expression of a retinol dehydrogenase (hRoDH-4), a member of the
            retinol/steroid dehydrogenase family implicated in retinoic acid
            biosynthesis, in normal and neoplastic endometria
  JOURNAL   Am J Obstet Gynecol 186 (4), 675-683 (2002)
   PUBMED   11967490
  REMARK    GeneRIF: In endometrial cancers, hRoDH-4 immunoreactivity was
            markedly reduced.
REFERENCE   5  (bases 1 to 1698)
  AUTHORS   Chetyrkin,S.V., Belyaeva,O.V., Gough,W.H. and Kedishvili,N.Y.
  TITLE     Characterization of a novel type of human microsomal 3alpha
            -hydroxysteroid dehydrogenase: unique tissue distribution and
            catalytic properties
  JOURNAL   J Biol Chem 276 (25), 22278-22286 (2001)
   PUBMED   11294878
REFERENCE   6  (bases 1 to 1698)
  AUTHORS   Jurukovski,V., Markova,N.G., Karaman-Jurukovska,N., Randolph,R.K.,
            Su,J., Napoli,J.L. and Simon,M.
  TITLE     Cloning and characterization of retinol dehydrogenase transcripts
            expressed in human epidermal keratinocytes
  JOURNAL   Mol Genet Metab 67 (1), 62-73 (1999)
   PUBMED   10329026
REFERENCE   7  (bases 1 to 1698)
  AUTHORS   Gough,W.H., VanOoteghem,S., Sint,T. and Kedishvili,N.Y.
  TITLE     cDNA cloning and characterization of a new human microsomal
            NAD+-dependent dehydrogenase that oxidizes all-trans-retinol and
            3alpha-hydroxysteroids
  JOURNAL   J Biol Chem 273 (31), 19778-19785 (1998)
   PUBMED   9677409
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AB209332.1, AF057034.1, AC026120.33 and AW449664.1.
            
            On Aug 11, 2020 this sequence version replaced NM_003708.4.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF086735.1, SRR5189652.257282.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2162895, SAMN04284274
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000398138.5/ ENSP00000381206.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                AB209332.1         1767-1776
            11-606              AF057034.1         1-596
            607-608             AC026120.33        56919-56920         c
            609-1136            AF057034.1         599-1126
            1137-1691           AB209332.1         2644-3198
            1692-1698           AW449664.1         15-21               c
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.3"
     gene            1..1698
                     /gene="RDH16"
                     /gene_synonym="hRDH-E; RODH-4; RODH4; SDR9C8"
                     /note="retinol dehydrogenase 16"
                     /db_xref="GeneID:8608"
                     /db_xref="HGNC:HGNC:29674"
                     /db_xref="MIM:620043"
     exon            1..459
                     /gene="RDH16"
                     /gene_synonym="hRDH-E; RODH-4; RODH4; SDR9C8"
                     /inference="alignment:Splign:2.1.0"
     CDS             147..1100
                     /gene="RDH16"
                     /gene_synonym="hRDH-E; RODH-4; RODH4; SDR9C8"
                     /EC_number="1.1.1.105"
                     /EC_number="1.1.1.315"
                     /EC_number="1.1.1.209"
                     /EC_number="1.1.1.53"
                     /note="isoform 1 is encoded by transcript variant 1;
                     microsomal NAD+-dependent retinol dehydrogenase 4; retinol
                     dehydrogenase 16 (all-trans and 13-cis); short chain
                     dehydrogenase/reductase family 9C, member 8;
                     sterol/retinol dehydrogenase; retinol dehydrogenase 16
                     (all-trans); human epidermal retinol dehydrogenase"
                     /codon_start=1
                     /product="retinol dehydrogenase 16 isoform 1"
                     /protein_id="NP_003699.3"
                     /db_xref="CCDS:CCDS41797.1"
                     /db_xref="GeneID:8608"
                     /db_xref="HGNC:HGNC:29674"
                     /db_xref="MIM:620043"
                     /translation="MWLYLAVFVGLYYLLHWYRERQVLSHLRDKYVFITGCDSGFGKL
                     LARQLDARGLRVLAACLTEKGAEQLRGQTSDRLETVTLDVTKTESVAAAAQWVKECVR
                     DKGLWGLVNNAGISLPTAPNELLTKQDFVTILDVNLLGVIDVTLSLLPLVRRARGRVV
                     NVSSVMGRVSLFGGGYCISKYGVEAFSDSLRRELSYFGVKVAMIEPGYFKTAVTSKER
                     FLKSFLEIWDRSSPEVKEAYGEKFVADYKKSAEQMEQKCTQDLSLVTNCMEHALIACH
                     PRTRYSAGWDAKLLYLPMSYMPTFLVDAIMYWVSPSPAKAL"
     exon            460..718
                     /gene="RDH16"
                     /gene_synonym="hRDH-E; RODH-4; RODH4; SDR9C8"
                     /inference="alignment:Splign:2.1.0"
     exon            719..882
                     /gene="RDH16"
                     /gene_synonym="hRDH-E; RODH-4; RODH4; SDR9C8"
                     /inference="alignment:Splign:2.1.0"
     exon            883..1698
                     /gene="RDH16"
                     /gene_synonym="hRDH-E; RODH-4; RODH4; SDR9C8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1670..1675
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RDH16"
                     /gene_synonym="hRDH-E; RODH-4; RODH4; SDR9C8"
                     /note="hexamer: AATAAA"
     polyA_site      1698
                     /gene="RDH16"
                     /gene_synonym="hRDH-E; RODH-4; RODH4; SDR9C8"
                     /note="major polyA site"
ORIGIN      
        1 agggaggaaa gtgaggattc cctgccaaaa tgcctgaggg cttccctgcc taccacagcc
       61 ctctgtgttc ttaaatcctc ctgtctgaac agaggccaga ctctggtttc ccccacagcc
      121 tgtctgtgtc tgtcctctgc aaagccatgt ggctctacct ggcggttttc gtgggcctgt
      181 actaccttct gcactggtac cgggagaggc aggtgctgag ccacctgaga gataagtatg
      241 tgttcatcac gggctgtgac tctggcttcg ggaaactgct ggccagacag ctggatgcac
      301 gaggcttgcg ggtgctggct gcatgtctga cggagaaagg agccgagcag ctgaggggcc
      361 agacttcaga caggctggag acggtgaccc tggatgttac caagacagag agcgttgctg
      421 cagccgccca gtgggtgaag gagtgcgtga gagacaaagg actctggggc ctggtgaata
      481 atgctggcat ctccttgccc acggctccca atgagttgct caccaagcag gacttcgtga
      541 ccatactgga cgtgaacttg ttgggggtga ttgatgtgac tctgagcctg ctgcccttag
      601 tgaggagggc caggggccgt gtggtcaacg tctccagtgt catgggccgg gtgtcacttt
      661 ttggtggagg ctactgcatc tccaagtatg gcgtggaagc cttctctgac tccctcagga
      721 gggaactctc ctactttggg gtgaaggtgg ctatgattga acctggctat ttcaagactg
      781 ctgtgaccag taaggagaga ttcttaaaga gcttcctgga gatttgggac cggtccagtc
      841 cagaggtcaa ggaggcctat ggcgagaagt ttgttgcaga ctataagaaa tcagctgaac
      901 aaatggagca gaagtgcaca caggatctgt cgttggtgac caactgcatg gagcatgcgc
      961 tgattgcctg ccacccccgt actcgctact cagctggctg ggatgccaag cttctctacc
     1021 tccccatgag ctacatgccc accttcctgg tggatgccat tatgtactgg gtctctccaa
     1081 gcccggccaa ggctctatga agctaaggtt ggatgcatgg ttgcatggat ttggggtgtg
     1141 ctatgagggg tggtgtatcc ttgggagaga tataaagtgg agggagggag ccgtccggtc
     1201 agtagggcac caatcccacc tccttcatta cctcctggcc atgattctcc tgggagataa
     1261 ttctgctctc tggagatgtt ggtaggaaag tttcaagtta cgcagctgag aaacagggac
     1321 caaatagtgc tcctgggtgc attgtcaccg tgggtggcca ctcaagggtc caagcctcta
     1381 gggccatcct tgggctaaca actggggtgg gtgtgagcag gtggaaggag cctcagccca
     1441 tgccattacc tcctgcttcc ttatcaggct gtgtgttaat tctgggccag tctacaccct
     1501 cccacggggt ggaaatggcc tggaggatgt gagggcaccc ctcctctgaa gatccctgta
     1561 cacgtggtgt tgggactgga accattatgc ggccccatag gcctcaggag tcatcccaga
     1621 agcagtggct gggaggtggt gtcctaagta aggatctgtg cagaggacaa ataaatcagt
     1681 ttttgatttg tcttgaaa
//
