LOCUS       NM_015436               4408 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens ring finger and CHY zinc finger domain containing 1
            (RCHY1), transcript variant 1, mRNA.
ACCESSION   NM_015436
VERSION     NM_015436.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Patil,P.R., Burroughs,A.M., Misra,M., Cerullo,F., Costas-Insua,C.,
            Hung,H.C., Dikic,I., Aravind,L. and Joazeiro,C.A.P.
  TITLE     Mechanism and evolutionary origins of alanine-tail C-degron
            recognition by E3 ligases Pirh2 and CRL2-KLHDC10
  JOURNAL   Cell Rep 42 (9), 113100 (2023)
   PUBMED   37676773
  REMARK    GeneRIF: Mechanism and evolutionary origins of alanine-tail
            C-degron recognition by E3 ligases Pirh2 and CRL2-KLHDC10.
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Sun,Y., Zhang,L., Fang,Z., Liu,D., Shao,M., Liu,Y., Liao,B. and
            Jin,Y.
  TITLE     PRPF8 controls alternative splicing of PIRH2 to modulate the p53
            pathway and survival of human ESCs
  JOURNAL   J Cell Physiol 238 (8), 1909-1920 (2023)
   PUBMED   37357506
  REMARK    GeneRIF: PRPF8 controls alternative splicing of PIRH2 to modulate
            the p53 pathway and survival of human ESCs.
REFERENCE   3  (bases 1 to 4408)
  AUTHORS   Abdelrahman,A.E., Salem,A., Al Attar,A.Z., Elsebai,E., Samy,W.,
            Ibrahim,M.A. and Ibrahim,H.M.
  TITLE     p53, Pirh2, and L1CAM as Promising Prognostic Biomarkers of
            Endometrial Carcinoma: An Immunohistochemical and Genetic Study
  JOURNAL   Appl Immunohistochem Mol Morphol 30 (10), 713-725 (2022)
   PUBMED   36251972
  REMARK    GeneRIF: p53, Pirh2, and L1CAM as Promising Prognostic Biomarkers
            of Endometrial Carcinoma: An Immunohistochemical and Genetic Study.
REFERENCE   4  (bases 1 to 4408)
  AUTHORS   Fedorova,O., Gudovich,A., Daks,A., Baidyuk,E., Shuvalov,O.,
            Petukhov,A., Parfenyev,S., Kizenko,A. and Barlev,N.A.
  TITLE     Regulation of autophagy flux by E3 ubiquitin ligase Pirh2 in lung
            cancer
  JOURNAL   Biochem Biophys Res Commun 563, 119-125 (2021)
   PUBMED   34090148
  REMARK    GeneRIF: Regulation of autophagy flux by E3 ubiquitin ligase Pirh2
            in lung cancer.
REFERENCE   5  (bases 1 to 4408)
  AUTHORS   Daks,A., Petukhov,A., Fedorova,O., Shuvalov,O., Kizenko,A.,
            Tananykina,E., Vasileva,E., Semenov,O., Bottrill,A. and Barlev,N.
  TITLE     The RNA-binding protein HuR is a novel target of Pirh2 E3 ubiquitin
            ligase
  JOURNAL   Cell Death Dis 12 (6), 581 (2021)
   PUBMED   34091597
  REMARK    GeneRIF: The RNA-binding protein HuR is a novel target of Pirh2 E3
            ubiquitin ligase.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4408)
  AUTHORS   Duan,W., Gao,L., Druhan,L.J., Zhu,W.G., Morrison,C., Otterson,G.A.
            and Villalona-Calero,M.A.
  TITLE     Expression of Pirh2, a newly identified ubiquitin protein ligase,
            in lung cancer
  JOURNAL   J Natl Cancer Inst 96 (22), 1718-1721 (2004)
   PUBMED   15547185
  REMARK    GeneRIF: These results are consistent with the hypothesis that
            increased Pirh2 expression affects lung tumorigenesis by reducing
            p53 activity.
REFERENCE   7  (bases 1 to 4408)
  AUTHORS   Corcoran,C.A., Huang,Y. and Sheikh,M.S.
  TITLE     The p53 paddy wagon: COP1, Pirh2 and MDM2 are found resisting
            apoptosis and growth arrest
  JOURNAL   Cancer Biol Ther 3 (8), 721-725 (2004)
   PUBMED   15280670
REFERENCE   8  (bases 1 to 4408)
  AUTHORS   Logan,I.R., Sapountzi,V., Gaughan,L., Neal,D.E. and Robson,C.N.
  TITLE     Control of human PIRH2 protein stability: involvement of TIP60 and
            the proteosome
  JOURNAL   J Biol Chem 279 (12), 11696-11704 (2004)
   PUBMED   14701804
REFERENCE   9  (bases 1 to 4408)
  AUTHORS   Leng,R.P., Lin,Y., Ma,W., Wu,H., Lemmers,B., Chung,S., Parant,J.M.,
            Lozano,G., Hakem,R. and Benchimol,S.
  TITLE     Pirh2, a p53-induced ubiquitin-protein ligase, promotes p53
            degradation
  JOURNAL   Cell 112 (6), 779-791 (2003)
   PUBMED   12654245
  REMARK    GeneRIF: This protein is a p53-induced ubiquitin-protein ligase
            which promotes p53 degradation.
            GeneRIF: Pirh2 (ZNF363), a gene regulated by p53, encodes a RING-H2
            domain-containing protein with intrinsic ubiquitin-protein ligase
            activity. Pirh2 protein physically interacts with p53 and promotes
            ubiquitination and degradation of p53 independently of Mdm2.
REFERENCE   10 (bases 1 to 4408)
  AUTHORS   Beitel,L.K., Elhaji,Y.A., Lumbroso,R., Wing,S.S., Panet-Raymond,V.,
            Gottlieb,B., Pinsky,L. and Trifiro,M.A.
  TITLE     Cloning and characterization of an androgen receptor
            N-terminal-interacting protein with ubiquitin-protein ligase
            activity
  JOURNAL   J Mol Endocrinol 29 (1), 41-60 (2002)
   PUBMED   12200228
  REMARK    GeneRIF: cloning and characterization of an androgen receptor
            N-terminal-interacting protein with ubiquitin-protein ligase
            activity [androgen-receptor N-terminal-interacting protein; hARNIP]
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB044826.1, GQ250944.1,
            AF255666.1, AK308754.1, AK091501.1, AC096759.3, DN919417.1 and
            AW236209.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Sep 24, 2020 this sequence version replaced NM_015436.3.
            
            Summary: The protein encoded by this gene has ubiquitin ligase
            activity. It mediates E3-dependent ubiquitination and proteasomal
            degradation of target proteins, including tumor protein 53, histone
            deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus
            regulating their levels and cell cycle progression. Alternatively
            spliced transcript variants encoding different isoforms have been
            described for this gene. [provided by RefSeq, Jun 2013].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1, also known as Pirh2A).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK091501.1, SRR11853564.21045.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000324439.10/ ENSP00000321239.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-285               DB044826.1         285-569
            286-903             GQ250944.1         169-786
            904-1618            AF255666.1         813-1527
            1619-1972           AK308754.1         1379-1732
            1973-2701           AK091501.1         1973-2701
            2702-2702           AC096759.3         88232-88232         c
            2703-3212           AK091501.1         2703-3212
            3213-3213           AC096759.3         87721-87721         c
            3214-3791           AK091501.1         3214-3791
            3792-3845           AC096759.3         87089-87142         c
            3846-4303           DN919417.1         1-458               c
            4304-4402           AW236209.1         1-99                c
            4403-4408           AC096759.3         86526-86531         c
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q21.1"
     gene            1..4408
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
                     /note="ring finger and CHY zinc finger domain containing
                     1"
                     /db_xref="GeneID:25898"
                     /db_xref="HGNC:HGNC:17479"
                     /db_xref="MIM:607680"
     exon            1..207
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..903
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1; zinc
                     finger protein 363; androgen-receptor
                     N-terminal-interacting protein; ring finger and CHY zinc
                     finger domain containing 1, E3 ubiquitin protein ligase;
                     CH-rich interacting match with PLAG1; RING finger protein
                     199; E3 ubiquitin-protein ligase Pirh2; p53-induced
                     protein with a RING-H2 domain; zinc finger, CHY-type;
                     RING-type E3 ubiquitin transferase RCHY1"
                     /codon_start=1
                     /product="RING finger and CHY zinc finger
                     domain-containing protein 1 isoform 1"
                     /protein_id="NP_056251.2"
                     /db_xref="CCDS:CCDS3567.1"
                     /db_xref="GeneID:25898"
                     /db_xref="HGNC:HGNC:17479"
                     /db_xref="MIM:607680"
                     /translation="MAATAREDGASGQERGQRGCEHYDRGCLLKAPCCDKLYTCRLCH
                     DNNEDHQLDRFKVKEVQCINCEKIQHAQQTCEECSTLFGEYYCDICHLFDKDKKQYHC
                     ENCGICRIGPKEDFFHCLKCNLCLAMNLQGRHKCIENVSRQNCPICLEDIHTSRVVAH
                     VLPCGHLLHRTCYEEMLKEGYRCPLCMHSALDMTRYWRQLDDEVAQTPMPSEYQNMTV
                     DILCNDCNGRSTVQFHILGMKCKICESYNTAQAGGRRISLDQQ"
     exon            208..327
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
                     /inference="alignment:Splign:2.1.0"
     exon            328..443
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
                     /inference="alignment:Splign:2.1.0"
     exon            444..522
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
                     /inference="alignment:Splign:2.1.0"
     exon            523..567
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
                     /inference="alignment:Splign:2.1.0"
     exon            568..626
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
                     /inference="alignment:Splign:2.1.0"
     exon            627..653
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
                     /inference="alignment:Splign:2.1.0"
     exon            654..774
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
                     /inference="alignment:Splign:2.1.0"
     exon            775..4408
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1415..1420
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
                     /note="hexamer: AATAAA"
     polyA_site      1435
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
                     /note="major polyA site"
     regulatory      1594..1599
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
                     /note="hexamer: AATAAA"
     polyA_site      1618
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
                     /note="major polyA site"
     regulatory      4248..4253
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
                     /note="hexamer: ATTAAA"
     regulatory      4261..4266
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
                     /note="hexamer: AATAAA"
     polyA_site      4266
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
                     /note="major polyA site"
     polyA_site      4304
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
     regulatory      4380..4385
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
                     /note="hexamer: AGTAAA"
     polyA_site      4408
                     /gene="RCHY1"
                     /gene_synonym="ARNIP; CHIMP; PIRH2; PRO1996; RNF199; ZCHY;
                     ZNF363"
ORIGIN      
        1 atattgtcgc tagtggccgc tggggctggg aggggtgtgc tagagaggcg cagcttctct
       61 gggcgtggtt tttatcctgg gggaaggatc ggtggaatgt gaggggaggt ggaggagatg
      121 gcggcgacgg cccgggaaga tggcgccagc ggtcaagagc gaggtcagcg gggctgcgag
      181 cactatgaca gaggatgtct cctaaaggca ccttgctgtg acaagcttta tacttgccgc
      241 ttgtgtcatg ataacaatga agatcatcaa ctagatcgct ttaaagtgaa ggaagtgcag
      301 tgcataaact gtgaaaaaat tcaacatgcc caacagactt gtgaagaatg tagcacattg
      361 tttggagaat attattgcga tatatgccat ttgtttgaca aagataagaa gcagtatcac
      421 tgtgaaaact gtggaatttg taggattggt ccaaaggaag attttttcca ttgtttgaaa
      481 tgtaacttat gcctagctat gaatcttcaa ggaagacaca agtgtattga aaatgtgtcc
      541 cgacagaatt gtccaatatg tttggaggac attcacacat cccgtgttgt tgctcatgtc
      601 ttgccatgtg gacatctttt acatagaacg tgttatgaag aaatgttgaa agaaggctac
      661 agatgtccat tatgtatgca ctctgcttta gatatgacca ggtattggag acagctggat
      721 gatgaagtag cacagactcc tatgccatca gaatatcaga acatgactgt ggatattctc
      781 tgcaatgact gtaatggacg atccactgtt cagtttcata tattaggcat gaaatgtaag
      841 atttgtgaat cctataatac tgctcaagct ggaggacgta gaatttcact ggatcagcaa
      901 tgacgagcct acacagtact ggagaacttg gcattttctg atatagaaaa aggctttcct
      961 tggtatcgtg ttatcatgtg tcgtcatcta tgcattagag ttgatgtgtt ttgtactagt
     1021 gtcacagcat aaaatcatat tacaagtacg taaggattca gggtctcttg atagacttat
     1081 catagctcta tattgaatga aagccactgt gcttgtgttt tgattggaaa ttcctttagt
     1141 gtcagttttt tggaagccaa tgatgtttgc cactgataca ttcatagaag tgtattatat
     1201 tttcatgaag ctttgcttta agtgacacta cgcgtgcagt tcatttctag ttcttgatag
     1261 aactgcactt aattttcagt agtagtcttg actttttaag ggctgtgtta tattaagttc
     1321 tcccttactg ttaaaaccta attaaatatg ctttagaatt ttctaagaca gtcctctgga
     1381 tcctctaaat gtgaattttg atgtaataac tgctaataaa attatattga aactgtttta
     1441 tagaaagttt tttccaaagt tttctatgtt tggctcaatt agggcagtat catcagttct
     1501 ataatgtaaa agaaattttc tttcttttga ggtattccat aaaagtagtt gggaaaatta
     1561 ttatgtaaaa taataagact cttccaaagc cttaataaaa acatcaacat tgaaaacagt
     1621 tttttttagg ttgcccaaaa ttatttcttc aaagagtcat tcattccttc atttagcaag
     1681 tatttattga gcaactgcta tgtggcagat atgtttctag atgctgtaga gacaacagtg
     1741 aacaataaag ataaaagtcc ttgtcctcac agcagtacat tctattgttc atcatatttg
     1801 atcaatgtac tcatttttct ttatgcaagg agtggatgtc ttattgtaaa tgaatagaat
     1861 atttcttccc taagcgtgtg aaaatagggg atggatgagt gggaagggca tgggatattc
     1921 ctggcaagat ctctgatttc ataatacttt tttgggctca tgtagtggaa ttaaatatac
     1981 ctcttacttc aggaacaaag ttaacaaata tgtttgatga tccagccgac tttgacactg
     2041 ttatctccgt aatagcttta ccattaggct aggatgtaaa atgtacattt atcagttgat
     2101 aagtacctat tgagcaccag ttgtatttcc aactttgagc tatttgaatc ttctttgaat
     2161 aaggaagtta gccttttaac tagaaagcct aagagttctg gcacatttat ttcttctttt
     2221 gttgaaattt attcacctag ataaaggcag cagtttggga ctcagatctt tattttatcc
     2281 caccttcttg tcttctgtat tccactcttc ttatgttctt tcccttcatc tgtttccttg
     2341 gcctggccag agcagcagtg ctgatcttca tttgggaaag gatgttcctg ggtattacca
     2401 attagtatac agataagtaa agactagttt tccagatcta atactttttt ttgaagtggt
     2461 tttgctctgt catttaggct agagtgtagt gaagtgatcg tagctcactg cagccttgac
     2521 ttcctgggct gaactgatcc ttccacctca gcctcctgag tagctgggac tacaggtgtg
     2581 tgccaccatg cccagctaat ttttaattgt tttgtagaaa caaagtccag gctggtcttg
     2641 aactcctggg ctcaagcatt tctcctacct cggccttcca agggctagga ttacaggcat
     2701 gagccactgt gcttggtcca gatgtattac tttttatgtt tggtattatt ctgccaaacc
     2761 aatcatgtta ttgtttttaa tcattgaatc attggatctt taaataggag tgagtatata
     2821 ataataaggc tcttctgttt tcaacatctg tgctgggtca tgcattagca ttaattacta
     2881 caatgattct gtggggtaag tgttgtcctc atagacaaga aagctgaggt ttagaggtta
     2941 aaatgacaca ttgggggtaa ataacagaca ctatatgttg gagttgggaa tcagatccaa
     3001 gtttgactcc aaacccactt ttaccacatt gcttttgatc taaacttcta ttttgctaaa
     3061 atacccctca cagctagtca tccatatcat tgaagcgctc atgatttgat agattaacct
     3121 ttcatttttt taggtgactc tagttagaaa gtcttatttc acaatctgat tgtttcttta
     3181 acttaggatc caaacatgtc caaaataatg cagagtacat atgcaatcac tcgtttatgc
     3241 tgtctagtca tgcacttaaa gcaattttca aatgtactca aagtctctag gcttcggtac
     3301 ccagggttgc ttcaagtatt tgttcatgac acgtcaactc gatgattaaa atccttcaac
     3361 acttctcatt cacaataaga ataaaatcta catcccttac cattcagtat gcaaaactta
     3421 aaatacataa ctttaccctt aaatattttt tctttctctg acttcataag tataggttat
     3481 acctctataa gaaagaaggt aaaaaactct caacaatccc ttctttcaga gagaactact
     3541 gttaacattt taacagacaa cttcattctt ttcctattct tttttataat gcttacagga
     3601 atgttaaata cttagacaga tcttaagtac actagaatta tctgtccctg aaataagtta
     3661 ggttgccctg ccgcagaatt tgtatctata gtttatttgg attatttttg aaaaaagtaa
     3721 aataacaata cccttacgaa ttttttggtg catagcatct cagcttcttt tatcattaaa
     3781 gatgtatgta taaaaaaaaa atgagtgtat atcaagttgt tcatttctta gtttctgggg
     3841 tcttcttaac cagtacatag aactctttat gaattaactt tagcttatgt tggaagtagt
     3901 agtttgttta tagtgtcatg gattttaaaa tcaaactcag tttttatttc ttcactaaaa
     3961 cctattttca tatgatatac atgttaaatt tcccttttat tttggtcatg catcaaaata
     4021 aatttactaa gtactaaaga gccaacatta ttttataagt gcctggcaat aggtattcac
     4081 tagttgaata gtttaatttg aaatgaatta attttaaatg ggagttgttc aaaactatgc
     4141 cactttttct tttgtccttt gtgttattaa aatttaattg ataattgtgt atattatata
     4201 tatggagtac aatgtgatgt tttgatatat gtttacagtg tagaatgatt aaatcaatgt
     4261 aataaattta ttgcctcata ccattttttg tggtaaaaaa accacttaaa atctactctt
     4321 aatatttttg aaatgtacaa tgcattattt attatactca ccattctgtg taatagatca
     4381 gtaaagttta ttcctcttgt ctaactga
//
