LOCUS       NM_001100588            8964 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens ring finger and CCCH-type domains 2 (RC3H2),
            transcript variant 1, mRNA.
ACCESSION   NM_001100588 XM_005252055
VERSION     NM_001100588.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8964)
  AUTHORS   Zhou,M., Lu,W., Li,B., Yuan,X., Liu,M., Han,J., Liu,X. and Li,A.
  TITLE     Roquin2 suppresses breast cancer progression by inhibiting tumor
            angiogenesis via selectively destabilizing proangiogenic factors
            mRNA
  JOURNAL   Int J Biol Sci 17 (11), 2884-2898 (2021)
   PUBMED   34345214
  REMARK    GeneRIF: Roquin2 suppresses breast cancer progression by inhibiting
            tumor angiogenesis via selectively destabilizing proangiogenic
            factors mRNA.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8964)
  AUTHORS   Choi,J., Lee,K., Ingvarsdottir,K., Bonasio,R., Saraf,A.,
            Florens,L., Washburn,M.P., Tadros,S., Green,M.R. and Busino,L.
  TITLE     Loss of KLHL6 promotes diffuse large B-cell lymphoma growth and
            survival by stabilizing the mRNA decay factor roquin2
  JOURNAL   Nat Cell Biol 20 (5), 586-596 (2018)
   PUBMED   29695787
  REMARK    GeneRIF: Loss of KLHL6 promotes diffuse large B-cell lymphoma
            growth and survival by stabilizing the mRNA decay factor roquin2.
REFERENCE   3  (bases 1 to 8964)
  AUTHORS   Zhang,Q., Fan,L., Hou,F., Dong,A., Wang,Y.X. and Tong,Y.
  TITLE     New Insights into the RNA-Binding and E3 Ubiquitin Ligase
            Activities of Roquins
  JOURNAL   Sci Rep 5, 15660 (2015)
   PUBMED   26489670
  REMARK    GeneRIF: Crystal structures, small-angle X-ray scattering, and E2
            profiling revealed that while the two paralogs are highly
            homologous, RC3H2 and RC3H1 are different in their structures and
            functions.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8964)
  AUTHORS   Sakurai,S., Ohto,U. and Shimizu,T.
  TITLE     Structure of human Roquin-2 and its complex with constitutive-decay
            element RNA
  JOURNAL   Acta Crystallogr F Struct Biol Commun 71 (Pt 8), 1048-1054 (2015)
   PUBMED   26249698
  REMARK    GeneRIF: Roquin-1 and roquin-2 proteins function redundantly in
            mRNA degradation.
REFERENCE   5  (bases 1 to 8964)
  AUTHORS   Maruyama,T., Araki,T., Kawarazaki,Y., Naguro,I., Heynen,S.,
            Aza-Blanc,P., Ronai,Z., Matsuzawa,A. and Ichijo,H.
  TITLE     Roquin-2 promotes ubiquitin-mediated degradation of ASK1 to
            regulate stress responses
  JOURNAL   Sci Signal 7 (309), ra8 (2014)
   PUBMED   24448648
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8964)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   7  (bases 1 to 8964)
  AUTHORS   Castello,A., Fischer,B., Eichelbaum,K., Horos,R., Beckmann,B.M.,
            Strein,C., Davey,N.E., Humphreys,D.T., Preiss,T., Steinmetz,L.M.,
            Krijgsveld,J. and Hentze,M.W.
  TITLE     Insights into RNA biology from an atlas of mammalian mRNA-binding
            proteins
  JOURNAL   Cell 149 (6), 1393-1406 (2012)
   PUBMED   22658674
REFERENCE   8  (bases 1 to 8964)
  AUTHORS   Vaquerizas,J.M., Kummerfeld,S.K., Teichmann,S.A. and Luscombe,N.M.
  TITLE     A census of human transcription factors: function, expression and
            evolution
  JOURNAL   Nat Rev Genet 10 (4), 252-263 (2009)
   PUBMED   19274049
  REMARK    Review article
REFERENCE   9  (bases 1 to 8964)
  AUTHORS   Siess,D.C., Vedder,C.T., Merkens,L.S., Tanaka,T., Freed,A.C.,
            McCoy,S.L., Heinrich,M.C., Deffebach,M.E., Bennett,R.M. and
            Hefeneider,S.H.
  TITLE     A human gene coding for a membrane-associated nucleic acid-binding
            protein
  JOURNAL   J Biol Chem 275 (43), 33655-33662 (2000)
   PUBMED   10938276
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC007066.4 and AL359512.15.
            
            On Nov 22, 2018 this sequence version replaced NM_001100588.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853563.33863.1,
                                           SRR11853567.30819.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000357244.7/ ENSP00000349783.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-250               AC007066.4         139476-139725
            251-548             AC007066.4         147259-147556
            549-666             AC007066.4         151809-151926
            667-900             AC007066.4         154290-154523
            901-1076            AC007066.4         161456-161631
            1077-1277           AC007066.4         164041-164241
            1278-1410           AC007066.4         164710-164842
            1411-1529           AC007066.4         164962-165080
            1530-1642           AC007066.4         167252-167364
            1643-1951           AC007066.4         179178-179486
            1952-2166           AC007066.4         184704-184918
            2167-2600           AC007066.4         185733-186166
            2601-2771           AC007066.4         186742-186912
            2772-2918           AL359512.15        58598-58744         c
            2919-3132           AL359512.15        58050-58263         c
            3133-3217           AL359512.15        57397-57481         c
            3218-3299           AL359512.15        57051-57132         c
            3300-3434           AL359512.15        56818-56952         c
            3435-3548           AL359512.15        54189-54302         c
            3549-3697           AL359512.15        53947-54095         c
            3698-8964           AL359512.15        47422-52688         c
FEATURES             Location/Qualifiers
     source          1..8964
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q33.2"
     gene            1..8964
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /note="ring finger and CCCH-type domains 2"
                     /db_xref="GeneID:54542"
                     /db_xref="HGNC:HGNC:21461"
                     /db_xref="MIM:615231"
     exon            1..250
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     exon            251..548
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     CDS             318..3893
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1;
                     membrane associated DNA binding protein; roquin-2; ring
                     finger and CCCH-type zinc finger domains 2; ring finger
                     and CCCH-type zinc finger domain-containing protein 2;
                     RING finger protein 164; membrane-associated nucleic
                     acid-binding protein; RING-type E3 ubiquitin transferase
                     Roquin-2"
                     /codon_start=1
                     /product="roquin-2 isoform 1"
                     /protein_id="NP_001094058.1"
                     /db_xref="CCDS:CCDS43874.1"
                     /db_xref="GeneID:54542"
                     /db_xref="HGNC:HGNC:21461"
                     /db_xref="MIM:615231"
                     /translation="MPVQAAQWTEFLSCPICYNEFDENVHKPISLGCSHTVCKTCLNK
                     LHRKACPFDQTAINTDIDVLPVNFALLQLVGAQVPDHQSIKLSNLGENKHYEVAKKCV
                     EDLALYLKPLSGGKGVASLNQSALSRPMQRKLVTLVNCQLVEEEGRVRAMRAARSLGE
                     RTVTELILQHQNPQQLSANLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALSRKV
                     LVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRSYEAL
                     RREHDAQIVHIAMEAGLRISPEQWSSLLYGDLAHKSHMQSIIDKLQSPESFAKSVQEL
                     TIVLQRTGDPANLNRLRPHLELLANIDPNPDAVSPTWEQLENAMVAVKTVVHGLVDFI
                     QNYSRKGHETPQPQPNSKYKTSMCRDLRQQGGCPRGTNCTFAHSQEELEKYRLRNKKI
                     NATVRTFPLLNKVGVNNTVTTTAGNVISVIGSTETTGKIVPSTNGISNAENSVSQLIS
                     RSTDSTLRALETVKKVGKVGANGQNAAGPSADSVTENKIGSPPKTPVSNVAATSAGPS
                     NVGTELNSVPQKSSPFLTRVPVYPPHSENIQYFQDPRTQIPFEVPQYPQTGYYPPPPT
                     VPAGVAPCVPRFVRSNNVPESSLPPASMPYADHYSTFSPRDRMNSSPYQPPPPQPYGP
                     VPPVPSGMYAPVYDSRRIWRPPMYQRDDIIRSNSLPPMDVMHSSVYQTSLRERYNSLD
                     GYYSVACQPPSEPRTTVPLPREPCGHLKTSCEEQIRRKPDQWAQYHTQKAPLVSSTLP
                     VATQSPTPPSPLFSVDFRADFSESVSGTKFEEDHLSHYSPWSCGTIGSCINAIDSEPK
                     DVIANSNAVLMDLDSGDVKRRVHLFETQRRTKEEDPIIPFSDGPIISKWGAISRSSRT
                     GYHTTDPVQATASQGSATKPISVSDYVPYVNAVDSRWSSYGNEATSSAHYVERDRFIV
                     TDLSGHRKHSSTGDLLSLELQQAKSNSLLLQREANALAMQQKWNSLDEGRHLTLNLLS
                     KEIELRNGELQSDYTEDATDTKPDRDIELELSALDTDEPDGQSEPIEEILDIQLGISS
                     QNDQLLNGMAVENGHPVQQHQKEPPKQKKQSLGEDHVILEEQKTILPVTSCFSQPLPV
                     SISNASCLPITTSVSAGNLILKTHVMSEDKNDFLKPVANGKMVNS"
     exon            549..666
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     exon            667..900
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1076
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1277
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1410
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1529
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     exon            1530..1642
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     exon            1643..1951
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     exon            1952..2166
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     exon            2167..2600
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     exon            2601..2771
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     exon            2772..2918
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     exon            2919..3132
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     exon            3133..3217
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     exon            3218..3299
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     exon            3300..3434
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     exon            3435..3548
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     exon            3549..3697
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     exon            3698..8964
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8939..8944
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /note="hexamer: AATAAA"
     polyA_site      8964
                     /gene="RC3H2"
                     /gene_synonym="MNAB; RNF164"
                     /note="major polyA site"
ORIGIN      
        1 atccctccag gtccgcctcc gtggtgagga ggaggaggag gaggaggcgg aggtggaggg
       61 aggaggagga ggccccgtcg cggccttcgc cgccgccaac ggggctgtcc ctgtagctcc
      121 cgatggagtt tgaggtcgtg aaacctccgc cgagctctgc cccggcgaga cgaggcgcct
      181 ccgtcgccaa gccgcgccgt cgcggggctc ccgggagcgg cccttagcga ccccgcccgg
      241 gccccctcag cttacaaaac actacacagc aaaataatga tctgctagac tgctaacccg
      301 agcatccagc ttccacaatg cctgtgcagg cagctcaatg gacagaattt ctgtcctgtc
      361 caatctgcta taatgaattt gatgagaatg tgcacaaacc catcagttta ggttgttcac
      421 acactgtttg caagacctgc ttgaataaac ttcatcgaaa agcttgtcct tttgaccaga
      481 ctgccatcaa cacagatatt gatgtacttc ctgtcaactt cgcacttctc cagttagttg
      541 gagcccaggt accagatcat cagtcaatta agttaagtaa tctaggtgag aataaacact
      601 atgaggttgc aaagaaatgc gttgaggatt tggcactcta cttaaaacca ctaagtggag
      661 gtaaaggtgt agctagcttg aaccagagtg cactgagccg tccaatgcaa aggaaactgg
      721 tgacacttgt aaactgtcaa ctggtggagg aagaaggtcg tgtaagagcc atgcgagcag
      781 ctcgttccct tggagaaaga actgtaacag aactgatatt acagcaccag aaccctcagc
      841 agttgtctgc caatctatgg gccgctgtca gggctcgagg atgccagttt ttagggccag
      901 ctatgcaaga agaggccttg aagctggtgt tactggcatt agaagatggt tctgccctct
      961 caaggaaagt tctggtactt tttgttgtgc agagactaga accaagattt cctcaggcat
     1021 caaaaacaag tattggtcat gttgtgcaac tactgtatcg agcttcttgt tttaaggtta
     1081 ccaaaagaga tgaagactct tccctaatgc agctgaagga ggaatttcgg agttatgaag
     1141 cattacgcag agaacatgat gcccaaattg ttcatattgc catggaagca ggactccgta
     1201 tttcacctga acagtggtcc tctcttttgt atggtgattt ggctcataaa tcacacatgc
     1261 agtctatcat tgataagcta cagtctccag agtcatttgc aaagagtgtc caggaattga
     1321 caattgtttt gcaacgaaca ggtgacccag ctaacttaaa tagactgagg cctcatttag
     1381 agcttcttgc aaacatagac cctaatccag acgctgtttc accaacttgg gagcagctgg
     1441 aaaatgcaat ggtagctgtt aaaacagtag ttcatggcct tgtggacttc atacaaaatt
     1501 atagtagaaa aggccatgag acccctcagc ctcagccaaa cagcaaatac aagactagca
     1561 tgtgccgaga tttgcgacag caagggggtt gtccacgagg aacaaattgt acatttgccc
     1621 attctcagga agagcttgaa aagtatcgat taaggaacaa aaagatcaat gccactgtaa
     1681 gaacgtttcc tcttctaaat aaagttggtg taaacaacac tgtcacaacc acagccggaa
     1741 atgtcatttc tgtcatagga agtactgaaa caacagggaa aattgttcca agtacaaacg
     1801 gaatttcaaa tgcagaaaac agtgtttccc agctaatctc acgtagtact gacagtacct
     1861 taagagctct ggagaccgtg aagaaagtgg gaaaggttgg cgctaatggt cagaatgctg
     1921 ctgggccctc tgcagattct gtaactgaaa ataaaattgg ttctccaccc aagactcctg
     1981 taagtaatgt agcagctacc tcagctgggc cctctaatgt tggaacagag ctgaattctg
     2041 tgcctcaaaa atccagccca tttctaacta gagtaccagt atatcctccg cattctgaaa
     2101 acattcagta ttttcaagat ccaaggactc agataccctt tgaagtccca cagtacccac
     2161 agacaggata ctatccacca cctccaacgg taccagctgg tgtggctccc tgtgttcctc
     2221 gctttgtgag gtccaataac gttccagagt cctccctccc acctgcttcc atgccatatg
     2281 ccgatcatta cagtacattt tcccctcgag atcgaatgaa ttcttctcct taccagcctc
     2341 ctcctccgca gccgtatgga ccagttcctc cagtaccttc tggaatgtat gctcctgtgt
     2401 acgacagcag gcgcatctgg cgcccaccta tgtaccaacg agatgacatt attagaagca
     2461 attctttacc tccaatggat gtgatgcact catctgtcta tcagacatct ttgcgggaaa
     2521 gatataactc attagatgga tattattcgg tggcttgtca gccaccaagt gagccaagga
     2581 caactgtgcc tttaccaagg gaaccttgtg gtcatttgaa gaccagttgc gaggagcaga
     2641 taagaagaaa gccagatcag tgggcacagt accacactca gaaagcacct cttgtctctt
     2701 caactcttcc tgtggcaaca cagtcaccaa caccaccttc tcctctgttc agtgtagact
     2761 ttcgtgcgga tttctcagag agtgtgagtg gtacaaaatt tgaagaagat catctttccc
     2821 attattctcc ctggtcttgt ggcaccatag gctcctgtat aaatgccatt gattcagagc
     2881 ccaaagatgt cattgctaat tcaaatgctg tgttaatgga cctggacagt ggtgatgtta
     2941 agagaagagt acatttattt gaaacccaga gaaggacaaa agaagaagat ccaataattc
     3001 cctttagtga tggacccatc atctcaaaat ggggtgcgat ttccagatct tcccgtacag
     3061 gttaccatac cacagatcct gtccaggcca ctgcttccca aggaagtgcg actaagccca
     3121 tcagtgtatc agattatgtc ccttatgtca atgctgttga ttcaaggtgg agttcatatg
     3181 gcaacgaggc cacatcatca gcacactatg ttgaaaggga cagattcatt gttactgatt
     3241 tatctggtca tagaaagcat tccagtactg gggacctttt gagccttgaa cttcagcagg
     3301 ccaagagcaa ctcattactt cttcagagag aggccaatgc tttggccatg caacagaagt
     3361 ggaattccct ggatgaaggc cgtcacctta ccttaaacct tttaagcaag gaaattgaac
     3421 taagaaatgg agagttacag agtgattata cagaagatgc aacagatact aaacctgata
     3481 gggatatcga gttagagctt tcagcacttg atactgatga acctgatgga caaagtgaac
     3541 caattgaaga gatcttggac atacagcttg gtatcagttc tcaaaatgat cagttgctaa
     3601 atggaatggc agtggaaaat gggcatccag tacagcagca ccaaaaggag ccaccaaagc
     3661 agaagaaaca gagtttaggt gaagaccatg tgattctgga ggagcaaaaa acaattctgc
     3721 cggtaacttc ttgctttagc cagccactcc cagtgtctat tagcaatgca agttgcctcc
     3781 ccatcaccac atctgtcagt gctggcaacc tcattctgaa aactcatgtt atgtctgaag
     3841 ataaaaacga ctttttaaaa cctgttgcaa atgggaagat ggttaacagc tgaaaggagg
     3901 ttcatctttc aaatttgtga ccacaccatg gaagcattta cactagcttt ttatatatat
     3961 aatatatatt atataatgta tatttttttt aaaaaaaaga tattactggg ggcatccatt
     4021 tcctgtggac tctttgatac ttcaagccct cttgcattag cattatgaaa aaaaaaaaga
     4081 aaatttactt tactagggta acatgttcac tttccagaaa tgattggaat ttggacaaca
     4141 tttaacttaa gcttcaagca gctttttatg agtttgtagc aatccggtgc agtaactgag
     4201 ccatttccta ttttaaatgg cttctataga aaattaacaa ctcagttatt aaactagcag
     4261 gatcctacaa tgatacatct agtgaaagta gacttagtta acttatttat ttctatttta
     4321 tgtttcactg agagaagttt ccatctcatt ccagctgctt tatttatctt tttcatggaa
     4381 ctttgcatgg atttcttttt ctttttttaa ttttgaagag tggagttaga tgttcttacc
     4441 atagaatttt acagggttat atactagcat tctttactgc attatatgta ggagtgtggt
     4501 gcagtgcttt tatctgtagc atataatttt tttttaattt tccatttttc aagagtagtt
     4561 tctgctttgt taatatttat acacaggcta cttgttgaag taattaatta aaaatataac
     4621 caagtgccta agtctttcag ctgatgtact agatattaaa ttctcctaag ttatgcagaa
     4681 tcgtttttac aattttgata aattatgcac taatgtaatg gcagtttttt aaaatgcaga
     4741 tttaagcctg tacattattg aatctgatga cttggcaaaa gaatcaggtg ctttcagctt
     4801 tcttgagaaa accctgtatg tagcgtttgc actgactaac aagatggtat tgctgggttt
     4861 ttaatttaaa ctaattaatt tggttgttca ttgcattatc ttggttaaat attgtttatt
     4921 gttacttcat gttggggttt agtttttctt gttttttgac tgttaggttg ataagactat
     4981 gaactgtgta atagtagaac agtggctaac atttattcat acttaagaac atgaataatt
     5041 tctgccttgc taaaatgtga ctgaaatggt tttgacttgg catctgaaag tatacaatgt
     5101 gttcaacaag tcatgcatca gatttgtggg aggtattgta ctgaaaccta aatttcagtc
     5161 aacatttgaa aagaagagct gggcattcct tcctggtgat ctttacctat agtttatcta
     5221 tctgtttttc ttctgtttct ttaagattag tttgactttt attattattt ttttaattaa
     5281 cttctgtgaa gttgtttact ctgaaaattg tactggaata ttttaagcca agctgatctt
     5341 tcaccaggtg tactgtatgt aagggctttt cctgctagca agatgcttaa acaggatcat
     5401 gttattggtc gtctgagcta tttagttgtt ttacaaaacc acagcattgt atcagataag
     5461 attttgataa aaagttttgt gcacatttcc aggggcggga gggttgacat ttccctgaaa
     5521 tttcttgatt agaatgagta aatactggtg tttgatacct gtcttaatga acatgctcat
     5581 aaggtgactg ataagttgta aatatacctg agaaacaaag gggtagtttt gatattctgg
     5641 tgtcagtatg gaagatctta cacatttatt taatactcaa atgtatgaag atgtttgttt
     5701 gtagcataac aacagcacag tagccttgac ttagtttcct ttggttagta ctgtaccttt
     5761 tttgccatgg ccagtgctcc ccatccctat gaagacattt tatttatttc actctgtaac
     5821 ctgaaatgaa taggaacaag ggttttaaac catgttacat taacttttct gacattataa
     5881 attagcctag aatattacag gaacatgatt gttatataat ttatatcaga acctttctat
     5941 aaatatgtgc ttattacatt tgaaatgctt ccaatgtact ttatttgctg tttgtaattc
     6001 caaaaaggat atgcaaacca gtctgtctat ttcatggata tttaatagtg agatcttcca
     6061 tgttgaaggt aatgtatttt tttttaagat tttaaaattg ctattttgtt acaaaataga
     6121 gacctattaa cagtttgaga gctccttatg tgccctcagg gaaagaaccc tctgtgcaac
     6181 agaactacac aaaacatctt cagcacgttc tgagggtgaa tatatactac ataaattgat
     6241 cttgtgtatt taaccttatc tttttaattt taaaattgaa attttgaaac ttggttgtgc
     6301 tctgctggag ggggttggga taaactgctt agatgtttgc ctacttgtcc agtgaaatta
     6361 catttgcatc agtatatgta tatacctgcc aaccttattg gtatgtctca gaacatttat
     6421 attaaaataa tgcttgtctt gcagcaggtg atcccataaa acaataactc cctgttctta
     6481 aaaacttact ctctttttca ctaacaacat aggaattgtt tctgtgttga gattattctg
     6541 tgttgcatcc attctctatg tagtggtcac cttctgtctg tcatggtggg cttaatgtga
     6601 agtaaagcca ttggttctct gccactttcg aagaaagggc agttcatctg atccttcttc
     6661 agaatggtac ctactgatat catcgaggaa aatgttacaa atcagaccta ggatatgact
     6721 ttttaaaatt gaggcatttc ccgtcatttg gcctttttac caacaagatg tgatgtacaa
     6781 atttggaatc aaaggataca aagcaacaag agaggaaatg tgtaatgtgg gtaaccaaac
     6841 tgcatatgtt acctattgtc taatcagaaa aaatagtctg aggtctggaa agcaggaaag
     6901 tggtggttat tttctttcat tttttgtgtt tttgtttttt aatttctaag ttccattctg
     6961 aaatccatgt gccgcattca gttactgtgg ttgtagcatc agtttttttg tggtttcatt
     7021 tcattttatg aattcagagg aagttacata tatatttttt ctagttggta cctgaacaaa
     7081 gcagattccc ttgcctttgc tgccacgtct tattttcaat gccatgtgat aaaccagtgt
     7141 catacatgaa agttgtgcat tgtaattttc aaaaaaaagg gagttcatta ggccagtggc
     7201 actgattttt ccttcccatc atagctattt attctagtaa gaggataata aaagaaattt
     7261 cctatacaag aactgaaatt ttccatttgt atggatctag tcactttcag atttcaattt
     7321 gaggttaagt atataaagca catcccaatt ttatatgctg ccttgagaaa attacaggat
     7381 gcacggcaat ttgtaggaat ttcaaatggg atcatttaaa catttgaaaa attattttaa
     7441 aaaccatcta gtttgctttt ggattttaga cattaaagcc tatgttgtct tgttaacagg
     7501 ggtggaatgt ataaccatca gattcagcat gtgatttcac ctttgaatct gagtatttct
     7561 tccctatctt ctttgagtca tttttggagc agactgtcac cagtattgat aactaagcat
     7621 taaagggaaa agttgcattg caactatgca ttggtttcct ggaagaactt ttcttttgtt
     7681 ttagtgaatg aagaggcttg atgggatcac ttactgtaac tccttctaca taaggacccc
     7741 ttctgcaagc agaacacaaa agaacatgct caaggagtat cccattttct ggataaattg
     7801 aagaagtttg ctagtaatgt ctttatacta gcgtcttcct tgtatccctt tgctggcaag
     7861 ggaatacaag gcgtcaagac cacagatcaa aacaccccac atttgagtgg agtcttattt
     7921 ttactccaag agcagttatt cccttctagt ctaaaattgg cagttttttc ttttttttaa
     7981 taaaattttt aaaatattcc caaaccagtg gaacacagac actggctgca cttagtactg
     8041 ccaaaagcca aggtcatttg cacatattcc atcaacctgt cgagaattag gcctcacttt
     8101 ataacccaag gcatggaagt gcatgcattc tcttagctgg gcaaacaatt atactgtagt
     8161 tgtgatacaa cacatgtggc ttttatttgt actgcacata tccactgtac agccacttgg
     8221 gagtatcgtg gttagcttgc agcaactgct gtctgcattt atactgttta ttgcatattc
     8281 ttttccctgg aagtgaaaga gaaatgtttt tcttgttgca ttgattacat tttataaatt
     8341 tgcttagctg gaaagtttgg gaaaagaggc ctgtttgtca attgtacaac cgattgtgaa
     8401 gctctagtgt gaatattttt acgtctgtat tagacatttt ctttgcaaat ctattgttcg
     8461 attgaaatgt aaatgaaatt aaagatggtg tacacccatc atgtaaaaag caggcaccat
     8521 ctctaagatg gatttaatgc tcatttttaa ggcatatact cagcttctat ttaaaactat
     8581 aatttaaaat aattctgtac aatgaaatgg ggaatatata tgggaataaa ttctattcca
     8641 tttatttcaa tttgaatttc caaattgtaa tgtttccctt tgtgctatag gaataggatt
     8701 aaatggggga agactaggat ttataaggcc tgtatatggg gggagggcag agatggaaca
     8761 atgagggttg tgatgatagt gaatagcaaa gagtgaattc tgtgtgtttt tgctgtagca
     8821 ctgaagtgaa gagatattag ctttggctgt tcacaaaata gagcatcatg attttcagtg
     8881 tttgagagaa aattgatgga aaaagtttgc agtacttgac atgtatttgc atgcacaaaa
     8941 taaaattatt tgtccacctt aaaa
//
