LOCUS       NM_172071              11451 bp    mRNA    linear   PRI 12-FEB-2025
DEFINITION  Homo sapiens ring finger and CCCH-type domains 1 (RC3H1),
            transcript variant 2, mRNA.
ACCESSION   NM_172071 XM_086409
VERSION     NM_172071.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Mai,D., Johnson,O., Reff,J., Fan,T.J., Scholler,J., Sheppard,N.C.
            and June,C.H.
  TITLE     Combined disruption of T cell inflammatory regulators Regnase-1 and
            Roquin-1 enhances antitumor activity of engineered human T cells
  JOURNAL   Proc Natl Acad Sci U S A 120 (12), e2218632120 (2023)
   PUBMED   36920923
  REMARK    GeneRIF: Combined disruption of T cell inflammatory regulators
            Regnase-1 and Roquin-1 enhances antitumor activity of engineered
            human T cells.
REFERENCE   2  (bases 1 to 11451)
  AUTHORS   Xia,S., Huang,J., Yan,L., Han,J., Zhang,W., Shao,H., Shen,H.,
            Wang,J., Wang,J., Tao,C., Wang,D. and Wu,F.
  TITLE     miR-150 promotes progressive T cell differentiation via inhibiting
            FOXP1 and RC3H1
  JOURNAL   Hum Immunol 83 (11), 778-788 (2022)
   PUBMED   35999072
  REMARK    GeneRIF: miR-150 promotes progressive T cell differentiation via
            inhibiting FOXP1 and RC3H1.
REFERENCE   3  (bases 1 to 11451)
  AUTHORS   O'Neill,A.C., Uzbas,F., Antognolli,G., Merino,F., Draganova,K.,
            Jack,A., Zhang,S., Pedini,G., Schessner,J.P., Cramer,K.,
            Schepers,A., Metzger,F., Esgleas,M., Smialowski,P., Guerrini,R.,
            Falk,S., Feederle,R., Freytag,S., Wang,Z., Bahlo,M., Jungmann,R.,
            Bagni,C., Borner,G.H.H., Robertson,S.P., Hauck,S.M. and Gotz,M.
  TITLE     Spatial centrosome proteome of human neural cells uncovers
            disease-relevant heterogeneity
  JOURNAL   Science 376 (6599), eabf9088 (2022)
   PUBMED   35709258
REFERENCE   4  (bases 1 to 11451)
  AUTHORS   Srivastava,R.K., Mishra,B., Muzaffar,S., Gorbatyuk,M.S.,
            Agarwal,A., Mukhtar,M.S. and Athar,M.
  TITLE     Dynamic Regulation of the Nexus Between Stress Granules, Roquin,
            and Regnase-1 Underlies the Molecular Pathogenesis of Warfare
            Vesicants
  JOURNAL   Front Immunol 12, 809365 (2022)
   PUBMED   35082795
  REMARK    GeneRIF: Dynamic Regulation of the Nexus Between Stress Granules,
            Roquin, and Regnase-1 Underlies the Molecular Pathogenesis of
            Warfare Vesicants.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 11451)
  AUTHORS   Lu,W., Zhou,M., Wang,B., Liu,X. and Li,B.
  TITLE     Roquin1 inhibits the proliferation of breast cancer cells by
            inducing G1/S cell cycle arrest via selectively destabilizing the
            mRNAs of cell cycle-promoting genes
  JOURNAL   J Exp Clin Cancer Res 39 (1), 255 (2020)
   PUBMED   33228782
  REMARK    GeneRIF: Roquin1 inhibits the proliferation of breast cancer cells
            by inducing G1/S cell cycle arrest via selectively destabilizing
            the mRNAs of cell cycle-promoting genes.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 11451)
  AUTHORS   Heissmeyer,V. and Vogel,K.U.
  TITLE     Molecular control of Tfh-cell differentiation by Roquin family
            proteins
  JOURNAL   Immunol Rev 253 (1), 273-289 (2013)
   PUBMED   23550652
  REMARK    Review article
REFERENCE   7  (bases 1 to 11451)
  AUTHORS   Athanasopoulos,V., Barker,A., Yu,D., Tan,A.H., Srivastava,M.,
            Contreras,N., Wang,J., Lam,K.P., Brown,S.H., Goodnow,C.C.,
            Dixon,N.E., Leedman,P.J., Saint,R. and Vinuesa,C.G.
  TITLE     The ROQUIN family of proteins localizes to stress granules via the
            ROQ domain and binds target mRNAs
  JOURNAL   FEBS J 277 (9), 2109-2127 (2010)
   PUBMED   20412057
REFERENCE   8  (bases 1 to 11451)
  AUTHORS   Linterman,M.A., Rigby,R.J., Wong,R., Silva,D., Withers,D.,
            Anderson,G., Verma,N.K., Brink,R., Hutloff,A., Goodnow,C.C. and
            Vinuesa,C.G.
  TITLE     Roquin differentiates the specialized functions of duplicated T
            cell costimulatory receptor genes CD28 and ICOS
  JOURNAL   Immunity 30 (2), 228-241 (2009)
   PUBMED   19217324
REFERENCE   9  (bases 1 to 11451)
  AUTHORS   Yu,D., Tan,A.H., Hu,X., Athanasopoulos,V., Simpson,N., Silva,D.G.,
            Hutloff,A., Giles,K.M., Leedman,P.J., Lam,K.P., Goodnow,C.C. and
            Vinuesa,C.G.
  TITLE     Roquin represses autoimmunity by limiting inducible T-cell
            co-stimulator messenger RNA
  JOURNAL   Nature 450 (7167), 299-303 (2007)
   PUBMED   18172933
  REMARK    Erratum:[Nature. 2008 Feb 21;451(7181):1022]
REFERENCE   10 (bases 1 to 11451)
  AUTHORS   Vinuesa,C.G., Cook,M.C., Angelucci,C., Athanasopoulos,V., Rui,L.,
            Hill,K.M., Yu,D., Domaschenz,H., Whittle,B., Lambe,T.,
            Roberts,I.S., Copley,R.R., Bell,J.I., Cornall,R.J. and Goodnow,C.C.
  TITLE     A RING-type ubiquitin ligase family member required to repress
            follicular helper T cells and autoimmunity
  JOURNAL   Nature 435 (7041), 452-458 (2005)
   PUBMED   15917799
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL121983.13, AB095945.1 and
            AL136170.14.
            
            On Aug 5, 2019 this sequence version replaced NM_172071.3.
            
            Summary: This gene encodes a protein containing RING-type and
            C3H1-type zinc finger motifs. The encoded protein recognizes and
            binds to a constitutive decay element (CDE) in the 3' UTR of mRNAs,
            leading to mRNA deadenylation and degradation. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Jul
            2014].
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the 3' coding region, compared to variant 1. The
            encoded isoform (b) is shorter than isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853564.2306.1,
                                           SRR18074967.858912.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000367696.7/ ENSP00000356669.2
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-262               AL121983.13        35391-35652         c
            263-484             AL121983.13        6209-6430           c
            485-5016            AB095945.1         1-4532
            5017-11451          AL136170.14        73720-80154         c
FEATURES             Location/Qualifiers
     source          1..11451
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q25.1"
     gene            1..11451
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /note="ring finger and CCCH-type domains 1"
                     /db_xref="GeneID:149041"
                     /db_xref="HGNC:HGNC:29434"
                     /db_xref="MIM:609424"
     exon            1..262
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     exon            263..643
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     CDS             413..3814
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /EC_number="2.3.2.27"
                     /note="isoform b is encoded by transcript variant 2; RING
                     finger protein 198; RING finger and C3H zinc finger
                     protein 1; probable E3 ubiquitin-protein ligase Roquin;
                     RING finger and CCCH-type zinc finger domain-containing
                     protein 1; roquin-1"
                     /codon_start=1
                     /product="roquin-1 isoform b"
                     /protein_id="NP_742068.1"
                     /db_xref="CCDS:CCDS30940.1"
                     /db_xref="GeneID:149041"
                     /db_xref="HGNC:HGNC:29434"
                     /db_xref="MIM:609424"
                     /translation="MPVQAPQWTDFLSCPICTQTFDETIRKPISLGCGHTVCKMCLNK
                     LHRKACPFDQTTINTDIELLPVNSALLQLVGAQVPEQQPITLCSGVEDTKHYEEAKKC
                     VEELALYLKPLSSARGVGLNSTTQSVLSRPMQRKLVTLVHCQLVEEEGRIRAMRAARS
                     LGERTVTELILQHQNPQQLSSNLWAAVRARGCQFLGPAMQEEALKLVLLALEDGSALS
                     RKVLVLFVVQRLEPRFPQASKTSIGHVVQLLYRASCFKVTKRDEDSSLMQLKEEFRTY
                     EALRREHDSQIVQIAMEAGLRIAPDQWSSLLYGDQSHKSHMQSIIDKLQTPASFAQSV
                     QELTIALQRTGDPANLNRLRPHLELLANIDPSPDAPPPTWEQLENGLVAVRTVVHGLV
                     DYIQNHSKKGADQQQPPQHSKYKTYMCRDMKQRGGCPRGASCTFAHSQEELEKFRKMN
                     KRLVPRRPLSASLGQLNEVGLPSAAILPDEGAVDLPSRKPPALPNGIVSTGNTVTQLI
                     PRGTDPSYDSSLKPGKIDHLSSSAPGSPPDLLESVPKSISALPVNPHSIPPRGPADLP
                     PMPVTKPLQMVPRGSQLYPAQQTDVYYQDPRGAAPPFEPAPYQQGMYYTPPPQCVSRF
                     VRPPPSAPEPAPPYLDHYPPYLQERVVNSQYGTQPQQYPPIYPSHYDGRRVYPAPSYT
                     REEIFRESPIPIEIPPAAVPSYVPESRERYQQIESYYPVAPHPTQIRPSYLREPPYSR
                     LPPPPQPHPSLDELHRRRKEIMAQLEERKVISPPPFAPSPTLPPTFHPEEFLDEDLKV
                     AGKYKGNDYSQYSPWSCDTIGSYIGTKDAKPKDVVAAGSVEMMNVESKGMRDQRLDLQ
                     RRAAETSDDDLIPFGDRPTVSRFGAISRTSKTIYQGAGPMQAMAPQGAPTKSINISDY
                     SPYGTHGGWGASPYSPHQNIPSQGHFSERERISMSEVASHGKPLPSAEREQLRLELQQ
                     LNHQISQQTQLRGLEAVSNRLVLQREANTLAGQSQPPPPPPPKWPGMISSEQLSLELH
                     QVEREIGKRTRELSMENQCSLDMKSKLNTSKQAENGQPEPQNKVPAEDLTLTFSDVPN
                     GSALTQENISLLSNKTSSLNLSEDPEGGGDNNDSQRSGVTPSSAP"
     exon            644..764
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     exon            765..1004
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1180
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1181..1381
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..1514
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1515..1633
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1634..1746
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     exon            1747..2028
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2029..2243
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2244..2614
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2615..2782
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2783..2935
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     exon            2936..3149
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3150..3240
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3241..3373
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3374..3547
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3548..3663
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     exon            3664..11451
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11305..11310
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /note="hexamer: GATAAA"
     polyA_site      11321
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /note="major polyA site"
     regulatory      11421..11426
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
                     /note="hexamer: AAGAAA"
     polyA_site      11451
                     /gene="RC3H1"
                     /gene_synonym="FHL6; IMDSHY; RNF198; ROQUIN"
ORIGIN      
        1 gacaacgagc gactgggcgg cggaggacga ggaggaggaa gaggaggccg agcaacaaga
       61 tggcccctgc tgcaggagca gcctcagcag cagcaggtgg gaggccggag gcggtgctgg
      121 cggtggcggc ggcagccgtg gcccggcggc ctgggacaga atcagagtca acggtggcct
      181 cggcggcggc ggcggctgcc gcggcggtgg cgacggccgc ggggactcgg agctgagagg
      241 agctgctgct cggaccagcg cggttatatt tcacactatg tgctgactgt atctacagaa
      301 gatatttaaa aaaaaaaaag ataaactttt tttcaaagat tttgattcca gtggaatctt
      361 tgctttagat ttgtgtgtgt gtttgtgttc gtgaattgta actccagaag caatgcctgt
      421 acaagctcca caatggacgg atttcctctc ctgcccaatt tgcactcaga ctttcgacga
      481 aacaattcga aagcccatca gtttgggttg tggccatact gtctgcaaga tgtgcctgaa
      541 taaactccac cgcaaggctt gcccatttga ccagaccact atcaatacag acattgagct
      601 cctccctgtg aactcagcat tgctgcagct cgtgggtgct caggtccctg agcagcagcc
      661 tattactttg tgtagtgggg ttgaagacac aaagcattat gaggaagcca agaaatgtgt
      721 agaagaatta gcattgtact taaaaccgct cagcagtgct agaggagtgg gtctgaacag
      781 cactactcag agtgttctga gtcgcccaat gcagaggaag cttgtgactc tagtccactg
      841 tcaactagta gaagaagaag gcaggattcg tgccatgagg gcagctcgat ctttaggtga
      901 acgaacagtt acagagctca ttctccagca ccagaatcct cagcaactct cttccaatct
      961 ttgggcagca gtaagggcta ggggatgcca gttccttgga ccagcaatgc aggaggaagc
     1021 tttaaagttg gttctgctgg ccttagaaga tggttctgct ttgtcaagaa aagtattggt
     1081 tctgtttgtg gtgcaaagat tggagccacg gtttcctcaa gcctctaaaa ctagcattgg
     1141 gcatgttgtt cagctccttt atagagcctc ctgtttcaag gtcaccaaac gtgatgaaga
     1201 ctcttctttg atgcagctga aagaagaatt tagaacctat gaagctctgc ggcgagaaca
     1261 tgactcccag atagtgcaga ttgctatgga agcaggctta cgaattgcac cggaccagtg
     1321 gtcctctttg ctttatggag accagtctca caaatctcat atgcagtcca ttattgacaa
     1381 gttgcagact ccagcctctt ttgcacagag tgttcaggaa ctaacaattg ctctccagcg
     1441 aactggagac ccagcaaact tgaaccgact aagaccccat ttggagctgt tagcaaacat
     1501 tgaccctagt ccagatgctc ctcctcctac ttgggaacag ctggaaaatg ggctggttgc
     1561 tgtgcgtaca gtggtacatg ggctggttga ttatatccag aaccacagca aaaaaggagc
     1621 agatcagcag cagcctccac agcatagcaa atacaaaaca tacatgtgtc gagatatgaa
     1681 gcagagagga ggatgccctc gtggggccag ctgtacattt gcacactcac aggaggaact
     1741 ggaaaaattt cgtaaaatga ataagcgcct ggttccgaga agacccttga gtgcctcttt
     1801 gggtcaactt aatgaggtgg gcctgccttc agcagctatc cttccagatg aaggtgcagt
     1861 ggatctccct agcagaaaac ctcctgctct gccaaatgga attgtatcaa cagggaatac
     1921 agtaacacag cttattccgc gagggacaga ccccagctat gattctagtc tgaaaccagg
     1981 aaaaatagat catctgagca gtagtgctcc tggatcccct cctgacctgc tagaatctgt
     2041 tcctaagagt atttctgcct tacctgtgaa tccacattct atacctccaa gggggccagc
     2101 agatctgcct ccaatgcctg ttaccaaacc acttcagatg gtacctcgag gttctcagtt
     2161 atatccagca caacagacgg atgtttatta tcaggatcct cgaggagcag ctccgccatt
     2221 tgaaccagca ccttatcagc agggtatgta ttatactcca ccaccacaat gtgtgtcccg
     2281 ctttgtccga cctccaccat ctgctcctga acctgctcct ccctacttgg atcattatcc
     2341 accctacctc caagaacgtg ttgtaaactc tcagtatggc acacagccac agcagtaccc
     2401 acctatatac ccatctcact atgatggccg tcgagtgtac cctgctccgt cttacacaag
     2461 agaagagata ttccgagaaa gccctatacc cattgagatt ccacctgcag cagtaccatc
     2521 gtatgtacca gaatccagag aaagatacca acagatcgag agttactatc cagtggctcc
     2581 tcatccaact cagatcagac cttcgtacct cagagaacct ccttatagcc ggcttcctcc
     2641 tcctccccag cctcatccta gtctagatga actacatcgc cgacgaaagg aaataatggc
     2701 ccagctagag gaaagaaagg ttatctctcc acctcctttt gcaccttcac caaccttgcc
     2761 tcctaccttt catccggaag aatttttgga tgaagacttg aaggtagctg ggaaatacaa
     2821 aggaaatgat tatagccaat actctccctg gtcatgtgac accatcggct cctacattgg
     2881 aaccaaagat gcaaaaccca aagatgttgt ggcagcaggg agtgtggaaa tgatgaatgt
     2941 ggagagtaaa ggaatgaggg accagcgatt agatcttcag agaagagcag cagaaaccag
     3001 tgatgatgac ctcatcccat ttggagaccg accaacagtg tctcggtttg gtgccatctc
     3061 acgaacttcc aaaactatat atcagggtgc tggtccaatg caggctatgg cacctcaggg
     3121 agctcctaca aaatctatta acatttcaga ttatagtcca tatggaaccc acggtggctg
     3181 gggagcttct ccatattcac ctcatcaaaa cataccttct cagggacact tcagtgagag
     3241 ggagagaata tctatgtcag aagtggccag tcatggaaaa ccccttccat ctgctgagag
     3301 agaacagcta cgactagaat tgcagcaatt gaaccatcag attagccagc agacccagct
     3361 acgtggacta gaggctgtta gtaacaggct ggtgttgcag agggaggcaa acaccctggc
     3421 aggccagtca cagcccccac caccgccacc tccaaaatgg cctgggatga tctcaagtga
     3481 gcagttgagc ttggaactgc accaggtgga aagggaaatc gggaagagaa cacgggaact
     3541 gagtatggaa aaccagtgtt ctctggacat gaaaagcaaa ctgaatacaa gtaaacaagc
     3601 agaaaatgga caaccagaac cacaaaacaa ggttccggct gaggacctta cattgacatt
     3661 cagtgatgta ccaaatggat cagccttgac acaagagaat atcagcctcc tatcaaacaa
     3721 gaccagctct ctgaacctgt cagaggaccc tgagggagga ggggataata atgactccca
     3781 gagatcagga gttactccca gttctgctcc ttaaaatatg aggagtccta cttttatctt
     3841 ctgctcctaa tcagtttgtc gggcataggg gtaggagaac gcataacttc taaacttttt
     3901 ctcttaagag cggtcagaga aatccaggaa attcaccaga ggcaatgtgc acaaacacca
     3961 ctactaaaaa caaaccctgc acatagttca cattaatgca tttttttaat ttaaagaaaa
     4021 agaaaattag cagtatctgc aagcaattca gattaaattt gtttttgttc tgtgaatgaa
     4081 ctttatttta ataactttgg acgccttgga tccagggctt taaaaaaaaa agatattata
     4141 tatacactct gtttgattaa ttgtatgatc ttaatgaagt aggttttctt gtattttggt
     4201 attacatacc cagtgtataa tttgtataat tcctcacccc caatccttat caattctcac
     4261 caccccataa acttaaaaca gaccaaaaat gtcagctttc agagaattct gtggtgcttg
     4321 agaaacagac agtactagac tgagttatta gaaaatgtgc agcaaaccaa cttttcccag
     4381 agctgttatt tggaattctt tatccctttc tagatcacta gcttcattgg atacgtatat
     4441 ttttcttgac attgaagctt tttgtttgtt tgggagggtt ttatttcttg tttttatttt
     4501 caacttttca aatagaatta ccaattttga agtcctcatt ttcatattaa ggatcatttg
     4561 cattctctta atataatcag aaagctgatt cagtgtttaa ttagatttaa atgttgatca
     4621 agaattaact gtaacttact agcactgaaa atacatgttt ggtgtttcgc ctgcgttcag
     4681 ctcatggttg gaaatttgaa gcagtgcttt tcaaattaaa cttttgctga catcatagga
     4741 tgctaaaaaa caaggacaat caactatgtt ttaaaactgc aacatttgat ttgaacttaa
     4801 aatgaataga gccacattat tttaatggat gttttgtttt acttgtgaac tgattgagtc
     4861 ttaaggatag tttatgaatt gatcacttgt tgctcagaat cattagtata agtaggtagg
     4921 aaatagtttg aagtcatttt tcatagactt aactgtccaa tgttgcaaat gtttcttcat
     4981 gaaaatattg aaaagagtaa ggtttttttt ttttttaatt ctgctgcaca tattctgatg
     5041 ccatgtgtct gtgcttaatt aactaagacc ctataaaacg tgtttagtgg ggactgtttg
     5101 atatagttgc cctctccatt cctctatttg cctgattttt ttcagtttcc ctttctgtat
     5161 tttactacta aattagaaag cactggttat gtaataagtt actacattgc tttggttgag
     5221 taaaaacaag agtttatatt tttcttgttt cttattttct taacccttaa ctcctgctga
     5281 ggtcataaca acctcagtct aagctctgtg tccatcatac tgttaactta aacaaatggg
     5341 tggtgggggt gggaagggta atacagtcct gccattgcct accagtagga gagtccaaac
     5401 agaacactct tttgagtagc aattatttaa tttgcccagt caaggcaccg tgtttatata
     5461 ctatttcaca ttgaatttga ttatgcccta cagacctggc tggtcaagga tttgatatac
     5521 acatattggc ttgggattcg agctttcttt tttatttaaa taaaaattta tatatatatt
     5581 atatatatac atatatacat agctatatct gtatatatat tgggtatgtt ttaaggattt
     5641 tttcgcatga gcgcagctgt tgcgataaat tgaccgattg ggaggtggta gaagcactga
     5701 atgaaggtga ggcttttttt ttttccagtt ttcttataca tttgtcttct ttttttaaaa
     5761 aaaaaaaaaa aaatatatat atatatatat atatatatat atatgtatgc tttttctttt
     5821 ttttttggct tttgtttgct ttttgacttt tacttaaaac ccttttacat ttccacttta
     5881 aatgcctatt gtttattggg agaaggaaag aaacttttgt atgtctgaca gagtcccaag
     5941 atttttcatt tctctgcata acagtaaaag gcaacattat tggcctccaa ccttcttttt
     6001 tttttttttt tttttttttt ggagagggag tctgcttctg ttgcccaggc tggagtgcag
     6061 tgggttgatc tcggctcgct acaacctcca cctcccgggt tcaagcgatt ctcctgcctc
     6121 agcctcctga gtagctggga ctacaggcac gcgccaccac gtccagctaa tttttgtact
     6181 acaaccttct tataagcaga tttggactga tcttctcctt tgggtatgtc agatcatcct
     6241 aaattttaga agcagttgat tcactttgga attcagaatg attcttgtat tagaagataa
     6301 tttgtgggta ttaatttttg tttaagttta aaataaaagt aaagattcat tttggatatc
     6361 agttgaaacc ccttagtaac tcagtttctg ttattcttgt tctcatttcc tttaaataca
     6421 cttgttcttg gcttttgcca ttttgattct gtgaagtagg caggagcagg gattaattta
     6481 tacagtattc ctgttctgaa caaaaccaga aaagtcactg tataaacttg acttaaaata
     6541 gtatctttct cttttcatgt attttcattt gggggaaaaa aaatctcttt aattgtaacc
     6601 tgaattcaag ctgtacccct ccatggtcct acactctaga gctaatctgg ttgggcagaa
     6661 aggcagaagg atggtatatt gtcccattgt gcctataatg tattttaaat tggtcattcc
     6721 accttaccta atggaaattc ttgcagcttt cctagtgctc atcagcggtt ttaggaattc
     6781 actaacaccc ccaaaatttg ttttttgttt tttgcttttt ttccttttcc ttttctctgt
     6841 gatatcatga gcctgtaagg acaaatggca tatttgggca taactgcttg actctgtaac
     6901 tactttttta aaatcaaaat tgaaaaggta ttaattgctt ctggcttttt aatttttttt
     6961 tttagagaca aagatgaaac cagataggaa atacagtttt tatgttttct acttactaaa
     7021 atattagttt cttctaaata ttactttatt tgtatttcac taaattccaa agcaaaaatc
     7081 agatatctga gatgaacaat tttgaatggg ttaaggtagg agtaataagc attgtgttca
     7141 ctcatacata ggtgagggaa ttttatttat agcgagaaat ataaaagtat tttgacttac
     7201 tcttttttca aggagtcgct aggaaagggt agaagaactg agaataagac atctagattc
     7261 catttctggt ttatatgtag ctcttttgcc taatttatat ataattttgt cataagataa
     7321 gtccagtaac tgtcctgtct tagataacgt acagaggctg aagtgaccaa agatccattc
     7381 cttattactt ttctaattaa aagaagagtt agctagattt ctagtagtag tattctagat
     7441 attgataaga cattattcct aatgccaact acaaatattc aactactcag aatgtattta
     7501 aaaaattgtt tggtacagca ttaatgttgg tgattccttt ctcccatctt atttcccttc
     7561 ccttctattc tttcccatgt atttcttttg cctaaaagaa tattggtatg attctcattt
     7621 gctactttga gtatataact ccatcagact ttccagagtt cttcagggat ggagctcaaa
     7681 gtttacattt ttccatatat gtcttttttt ttcttgacct ttttccaccc tgtaatttcc
     7741 ccttgactta cccactgccc tccccaatct gcaacagtga cagtttgctg cctcaaaaca
     7801 gagcattcaa atgaattcgc ttagccaata acttctgtga agttgccttt tctaatggtg
     7861 ttattactca gtgatgcaca aatgtggtat ttgccctact ggtaggcaaa caaaggtctg
     7921 gttaagaatg gtaacctgct gttttcttta aaggagagcc aaagacttgt gctttactct
     7981 gttttggttt tgggggaagt agtagccatt ttgatcatga aaccttttag tatttgtgag
     8041 ttgattgtac tgtgtaagta gatttatcta aagttaactg ttaaagttag ctagccttat
     8101 aaaatgtaaa agtaatgaaa attcatcagt ttctctaagc cagtgaatgt cgtcttttgt
     8161 aatattgtgt ataagcattt gtgcccaccc cagcttctgc tatcactgtg gtcaagtcat
     8221 gggtctgagt aaaagaattc ttgagtatgt gactggtgag aaataactaa ctatagtcag
     8281 tacatgtaca gattgttaaa attcaagaca aaatactttc actaaaaaaa aggattcgtc
     8341 tcagtaaaga agtattcagt tgctgtcact tacatacaaa tggtggtggg tagcagtctc
     8401 accatttttc tactctcttt ttgctcgttt taaataattg agtaaaggtt ttatttgtaa
     8461 aatctatctg taaaaagcac ctgtaacgat ttatatgtca ttgaaaggaa tggcccagtt
     8521 actttttagg agagattaat aacctacctg gaaattaact gcagtgttta cttagatttt
     8581 ttccccctaa taagagtcta agtgtgttaa atttttaact gatgatatgg ttacagtgtt
     8641 gcaaggtgat ctcttcccct gaaaaatctg cctatccttt taagaaaaat tagacctact
     8701 aaactaaagc aaattttact ttcatcatta gcacctaaac aagcaagcag tctactactg
     8761 tcatcatttt acctgagtgc ccattttgct gatccacatt catacctttc taccttggac
     8821 agctatgatt tttatattta tttgttgctt cagctcaggt ttatgaaatt ttcctgctga
     8881 aatgacttat tgggagttgt aggttgatta tagttactat taaacatttt ctcaatttag
     8941 cttttctgag ccacttcaag aagaaagctg tttttgtcaa aaggtactat taattgtagc
     9001 aagtaaatct taagatttga atttttaaga ttttatttct gtgttcacta aagatctttc
     9061 cacaagtaat agattataat tgataagtaa tactagctat agtgttaata aagagtaaga
     9121 ctatcaccac caggaataag taacaccttt taataataaa tctattccta ataggtatgt
     9181 tttcaatact gcactgaaca aagtgggtaa agagcccttt gaaatcttat tgtaataaag
     9241 ggacttacct caccactgga atccttgttt tcagagcctc tggattttca gccaaacact
     9301 gaatttctcc taaactttat ccctgaaaca tccctagaga acgaacttta ttttcacaag
     9361 aaaataaggc acaataaggg ggaagaactt aaacctcaac ataatatcgt gtccaaaaat
     9421 gtgtatttag aaaaataaaa tcctgtggag tttgttatcc taatgtttat tttatactag
     9481 ataaggtgac ttgttttctt gtattgaggt cttactgttt cagatttgtt tttgtttctg
     9541 ttctaactct aagcagacag agctcacaag tctttggcat ccattttagg ttgtacactc
     9601 agagtcatct tataactgga aactgtcagg tggtccccca ccccagtgag tgtatgaaag
     9661 agtcaagaaa aagattggaa acaaactatt gggtgaataa ttggttgctc tattattatt
     9721 attattatta tttgcctttt tttaatcacc atttatttgt gtaatctttc tccattgatt
     9781 ggctgtcatt cttataatat ctgaactacc ccataaaata attctctgac ttaaaggcat
     9841 ttagcttttg cttatcgttt ttacatcctc tattcaacta agacactgtc ttgagagtta
     9901 ttttttccag atggatcgtt ggcctaaatt ttcacacttc tcccctgttc atcctttttc
     9961 ctcttccctg cttcctggga ataaaaggaa cttttttaaa aaaattaatt agtccacagg
    10021 tctcattatc tttctttatg attaatctat gactttttgg tacaagaaca atggaaaaag
    10081 tgaattaagg taatgaacaa aacctttcac ccacttaaac attttccagt tttgagattc
    10141 ctcttcgtgt ttgtggtgtc ttccccttgt taccccttct gccctttttc tctgactatg
    10201 gtaatttggt ctttaggctc atatcagtct ccccgagaca ttctgcagtc attatcacct
    10261 ttttgggtgg attttatttt gttttatttt gtttttttta aaaaaataac tttttaacat
    10321 tggtgcatat ttgcttggga tagagcttgt gtaatttacc aatcgtattg attgtaagtg
    10381 attgtgccct gcagaggtat atttaacaag acaaaaataa tcttggttaa taaaggagcc
    10441 catgagattt gagtcaggtt gtaagtgaaa tcacttacac ttttggatag aatttatact
    10501 cctgctctta taaatcagtg gtagacttac cattttttaa agttttcttg catttttttg
    10561 tttttttatt gccacagctc cctattcttt cttgcctgcc tccacccccc tgttcaggaa
    10621 aaaaaaaaat tgagccttaa agtgacagct gattttttaa ttgctgaatt ttgtgaaatt
    10681 ttactttttc caagtgtttc caactttaaa aagagaagtg aagacaaata ggttggaatg
    10741 gtgaagacaa atggattgga atttcacagg ctgtgaataa ttccttagga tctggcaaac
    10801 cgtgaagtct tatttgaaga ccttatctcc tgagagttct tttggagtag gaaaaagaac
    10861 cctatttgaa atagaccgtt tttctcttgt ttttaatctg tttaatattt ctgattttta
    10921 agcagctttc aaaacaagtg tggtggaaaa aaagaaatag tagtaggaag atgtttaggg
    10981 cagcagaact ctgggtctaa ataagtacat gttcccactt gttgccgatt tttgagagta
    11041 ctagggccat ctttctcaat tttgtattat ttgtgtgcat gtttatatca aagatgccca
    11101 ttttgttaaa atgctatttc ctttattacc ttggaaactg actcagcctc atgttgctcc
    11161 taattagtgt ttaaggctcc catgagttgc agataaaatg atttatttta acaagtagaa
    11221 ggaggtgatt caccttttgg attgtaaata tatgaaaatg tctacaaggt ctttatctgc
    11281 tttctgtcag catttatatt aaatgataaa ttaatgagga acgtgtgtat tttgtaaatg
    11341 agtgtgtgcc atcttattct aatcttgaga ttactttctt acacgttttt aatggagggg
    11401 gcttttttag gtgaaaaata aagaaatggc taatctgcat gagcaaacac a
//
