LOCUS       NM_022340               6678 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens rabenosyn, RAB effector (RBSN), transcript variant 1,
            mRNA.
ACCESSION   NM_022340
VERSION     NM_022340.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6678)
  AUTHORS   Paul,F., Ng,C., Mohamad Sahari,U.B., Nafissi,S., Nilipoor,Y.,
            Tavasoli,A.R., Bonnard,C., Wong,P.M., Nabavizadeh,N., Altunoglu,U.,
            Estiar,M.A., Majoie,C.B., Lee,H., Nelson,S.F., Gan-Or,Z.,
            Rouleau,G.A., Van Veldhoven,P.P., Massie,R., Hennekam,R.C.,
            Kariminejad,A. and Reversade,B.
  TITLE     RABENOSYN separation-of-function mutations uncouple endosomal
            recycling from lysosomal degradation, causing a distinct Mendelian
            disorder
  JOURNAL   Hum Mol Genet 31 (21), 3729-3740 (2022)
   PUBMED   35652444
  REMARK    GeneRIF: RABENOSYN separation-of-function mutations uncouple
            endosomal recycling from lysosomal degradation, causing a distinct
            Mendelian disorder.
REFERENCE   2  (bases 1 to 6678)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 6678)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 6678)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 6678)
  AUTHORS   Stockler,S., Corvera,S., Lambright,D., Fogarty,K., Nosova,E.,
            Leonard,D., Steinfeld,R., Ackerley,C., Shyr,C., Au,N., Selby,K.,
            van Allen,M., Vallance,H., Wevers,R., Watkins,D., Rosenblatt,D.,
            Ross,C.J., Conibear,E., Wasserman,W. and van Karnebeek,C.
  TITLE     Single point mutation in Rabenosyn-5 in a female with intractable
            seizures and evidence of defective endocytotic trafficking
  JOURNAL   Orphanet J Rare Dis 9, 141 (2014)
   PUBMED   25233840
  REMARK    GeneRIF: A functional defect in the endocytic pathway resulting
            from a point mutation in Rabenosyn-5 leads to severe multi-organ
            disorder.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6678)
  AUTHORS   Beausoleil,S.A., Villen,J., Gerber,S.A., Rush,J. and Gygi,S.P.
  TITLE     A probability-based approach for high-throughput protein
            phosphorylation analysis and site localization
  JOURNAL   Nat Biotechnol 24 (10), 1285-1292 (2006)
   PUBMED   16964243
REFERENCE   7  (bases 1 to 6678)
  AUTHORS   Eathiraj,S., Pan,X., Ritacco,C. and Lambright,D.G.
  TITLE     Structural basis of family-wide Rab GTPase recognition by
            rabenosyn-5
  JOURNAL   Nature 436 (7049), 415-419 (2005)
   PUBMED   16034420
  REMARK    GeneRIF: results demonstrate that even the structurally similar
            effector domains in rabenosyn-5 can achieve highly selective
            recognition of distinct subsets of Rab GTPases exclusively through
            interactions with the switch and interswitch regions
REFERENCE   8  (bases 1 to 6678)
  AUTHORS   Naslavsky,N., Boehm,M., Backlund,P.S. Jr. and Caplan,S.
  TITLE     Rabenosyn-5 and EHD1 interact and sequentially regulate protein
            recycling to the plasma membrane
  JOURNAL   Mol Biol Cell 15 (5), 2410-2422 (2004)
   PUBMED   15020713
REFERENCE   9  (bases 1 to 6678)
  AUTHORS   de Renzis,S., Sonnichsen,B. and Zerial,M.
  TITLE     Divalent Rab effectors regulate the sub-compartmental organization
            and sorting of early endosomes
  JOURNAL   Nat Cell Biol 4 (2), 124-133 (2002)
   PUBMED   11788822
  REMARK    Erratum:[Nat Cell Biol. 2022 Dec;24(12):1776. doi:
            10.1038/s41556-022-01041-4.. PMID: 36471128]
REFERENCE   10 (bases 1 to 6678)
  AUTHORS   Nielsen,E., Christoforidis,S., Uttenweiler-Joseph,S.,
            Miaczynska,M., Dewitte,F., Wilm,M., Hoflack,B. and Zerial,M.
  TITLE     Rabenosyn-5, a novel Rab5 effector, is complexed with hVPS45 and
            recruited to endosomes through a FYVE finger domain
  JOURNAL   J Cell Biol 151 (3), 601-612 (2000)
   PUBMED   11062261
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA320360.1, AB209673.1,
            AY009133.1, BF513881.1, AC090954.3 and BX111409.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_022340.3.
            
            Summary: This gene encodes a protein that belongs to the FYVE zinc
            finger family of proteins. The encoded protein interacts with
            Ras-related proteins that regulate membrane trafficking. A missense
            mutation in this gene is associated with a defect in the early
            endocytic pathway. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Oct 2014].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AL833545.1, SRR1803617.150434.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000253699.7/ ENSP00000253699.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                DA320360.1         10-40
            32-2882             AB209673.1         1-2851
            2883-2969           AY009133.1         2269-2355
            2970-3350           BF513881.1         89-469
            3351-6175           AC090954.3         123890-126714
            6176-6678           BX111409.1         1-503
FEATURES             Location/Qualifiers
     source          1..6678
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p25.1"
     gene            1..6678
                     /gene="RBSN"
                     /gene_synonym="KAREVS; MFANDO; Rabenosyn-5; ZFYVE20"
                     /note="rabenosyn, RAB effector"
                     /db_xref="GeneID:64145"
                     /db_xref="HGNC:HGNC:20759"
                     /db_xref="MIM:609511"
     exon            1..114
                     /gene="RBSN"
                     /gene_synonym="KAREVS; MFANDO; Rabenosyn-5; ZFYVE20"
                     /inference="alignment:Splign:2.1.0"
     exon            115..270
                     /gene="RBSN"
                     /gene_synonym="KAREVS; MFANDO; Rabenosyn-5; ZFYVE20"
                     /inference="alignment:Splign:2.1.0"
     exon            271..446
                     /gene="RBSN"
                     /gene_synonym="KAREVS; MFANDO; Rabenosyn-5; ZFYVE20"
                     /inference="alignment:Splign:2.1.0"
     exon            447..762
                     /gene="RBSN"
                     /gene_synonym="KAREVS; MFANDO; Rabenosyn-5; ZFYVE20"
                     /inference="alignment:Splign:2.1.0"
     CDS             615..2969
                     /gene="RBSN"
                     /gene_synonym="KAREVS; MFANDO; Rabenosyn-5; ZFYVE20"
                     /note="110 kDa protein; FYVE finger-containing Rab5
                     effector protein rabenosyn-5; zinc finger, FYVE domain
                     containing 20; RAB effector RBSN"
                     /codon_start=1
                     /product="rabenosyn-5"
                     /protein_id="NP_071735.2"
                     /db_xref="CCDS:CCDS2623.1"
                     /db_xref="GeneID:64145"
                     /db_xref="HGNC:HGNC:20759"
                     /db_xref="MIM:609511"
                     /translation="MASLDDPGEVREGFLCPLCLKDLQSFYQLHSHYEEEHSGEDRDV
                     KGQIKSLVQKAKKAKDRLLKREGDDRAESGTQGYESFSYGGVDPYMWEPQELGAVRSH
                     LSDFKKHRAARIDHYVVEVNKLIIRLEKLTAFDRTNTESAKIRAIEKSVVPWVNDQDV
                     PFCPDCGNKFSIRNRRHHCRLCGSIMCKKCMELISLPLANKLTSASKESLSTHTSPSQ
                     SPNSVHGSRRGSISSMSSVSSVLDEKDDDRIRCCTHCKDTLLKREQQIDEKEHTPDIV
                     KLYEKLRLCMEKVDQKAPEYIRMAASLNAGETTYSLEHASDLRVEVQKVYELIDALSK
                     KILTLGLNQDPPPHPSNLRLQRMIRYSATLFVQEKLLGLMSLPTKEQFEELKKKRKEE
                     MERKRAVERQAALESQRRLEERQSGLASRAANGEVASLRRGPAPLRKAEGWLPLSGGQ
                     GQSEDSDPLLQQIHNITSFIRQAKAAGRMDEVRTLQENLRQLQDEYDQQQTEKAIELS
                     RRQAEEEDLQREQLQMLRERELEREREQFRVASLHTRTRSLDFREIGPFQLEPSREPR
                     THLAYALDLGSSPVPSSTAPKTPSLSSTQPTRVWSGPPAVGQERLPQSSMPQQHEGPS
                     LNPFDEEDLSSPMEEATTGPPAAGVSLDPSARILKEYNPFEEEDEEEEAVAGNPFIQP
                     DSPAPNPFSEEDEHPQQRLSSPLVPGNPFEEPTCINPFEMDSDSGPEAEEPIEEELLL
                     QQIDNIKAYIFDAKQCGRLDEVEVLTENLRELKHTLAKQKGGTD"
     exon            763..903
                     /gene="RBSN"
                     /gene_synonym="KAREVS; MFANDO; Rabenosyn-5; ZFYVE20"
                     /inference="alignment:Splign:2.1.0"
     exon            904..1004
                     /gene="RBSN"
                     /gene_synonym="KAREVS; MFANDO; Rabenosyn-5; ZFYVE20"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1050
                     /gene="RBSN"
                     /gene_synonym="KAREVS; MFANDO; Rabenosyn-5; ZFYVE20"
                     /inference="alignment:Splign:2.1.0"
     exon            1051..1212
                     /gene="RBSN"
                     /gene_synonym="KAREVS; MFANDO; Rabenosyn-5; ZFYVE20"
                     /inference="alignment:Splign:2.1.0"
     exon            1213..1454
                     /gene="RBSN"
                     /gene_synonym="KAREVS; MFANDO; Rabenosyn-5; ZFYVE20"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1525
                     /gene="RBSN"
                     /gene_synonym="KAREVS; MFANDO; Rabenosyn-5; ZFYVE20"
                     /inference="alignment:Splign:2.1.0"
     exon            1526..1612
                     /gene="RBSN"
                     /gene_synonym="KAREVS; MFANDO; Rabenosyn-5; ZFYVE20"
                     /inference="alignment:Splign:2.1.0"
     exon            1613..1715
                     /gene="RBSN"
                     /gene_synonym="KAREVS; MFANDO; Rabenosyn-5; ZFYVE20"
                     /inference="alignment:Splign:2.1.0"
     exon            1716..1820
                     /gene="RBSN"
                     /gene_synonym="KAREVS; MFANDO; Rabenosyn-5; ZFYVE20"
                     /inference="alignment:Splign:2.1.0"
     exon            1821..6678
                     /gene="RBSN"
                     /gene_synonym="KAREVS; MFANDO; Rabenosyn-5; ZFYVE20"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6660..6665
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RBSN"
                     /gene_synonym="KAREVS; MFANDO; Rabenosyn-5; ZFYVE20"
                     /note="hexamer: AATAAA"
     polyA_site      6678
                     /gene="RBSN"
                     /gene_synonym="KAREVS; MFANDO; Rabenosyn-5; ZFYVE20"
                     /note="major polyA site"
ORIGIN      
        1 gagttccgag cgaccgatgg agatggcggc tgcggctgag tgacggacgg tggaggccca
       61 gagcccgggc ctgaaggggg ggacaaacct gggtgcccgc aggagcccgg cagggtactg
      121 tgctaaaccc tggggagacg gcgaagtgga aaacatatag agtctgtgcc ctcccagatt
      181 ttacagtctt acttggtcac ttcacgtatg tacctgcggt tataaattga gatgagtgct
      241 gtgaaggaga agtccattct gatgctctga gtgtcttaca agtatcaaga acttactata
      301 tgtggttgaa taaacaatca aggtaaagag catcaagtaa aaacttctgc ttgttgataa
      361 gtacttcaga cattccccca gtggctgaag tggcatatga attatgaagt tggatcattt
      421 ggaatgaatg taagagaatt gccaagggct cctcctactc cagagaggaa acctcatcca
      481 gggccatgaa gccacttcct caccatctgt gtgctgctta agctaatgct gcgggaacca
      541 tggttccttg ggaggaatca agctgactct tggcatgaga ttcctgcctt cctagggttg
      601 agagcggcac tgccatggct tctctggacg acccagggga agtgagggag ggcttcctct
      661 gccctctgtg cctgaaggat ctgcagtctt tctatcagct tcactcacat tacgaggaag
      721 aacactcagg ggaagaccgt gatgtcaaag ggcaaattaa aagtcttgtc cagaaggcta
      781 aaaaagcaaa ggacaggttg ttgaaacgag aaggggatga tcgagcagag tcagggaccc
      841 aaggatatga gtctttcagc tatggagggg ttgatcctta catgtgggaa ccccaggagc
      901 ttggtgctgt gagaagccat ctttccgact tcaaaaaaca ccgagctgct agaattgacc
      961 actatgttgt ggaagtcaat aaactaataa tcaggttaga gaagctcact gcatttgaca
     1021 gaacaaatac tgagtctgca aagattcgag caatagaaaa gtctgtggtg ccttgggtca
     1081 acgaccagga tgtccctttc tgtccagact gtgggaataa gttcagcatc cggaaccgcc
     1141 gccaccactg ccgcctctgc gggtctatta tgtgcaagaa gtgtatggag ctcatcagcc
     1201 ttcccttggc aaacaagctc accagtgcca gcaaggagtc cctgagcacc cacaccagcc
     1261 ccagccagtc acccaacagt gtccatggct cccgccgagg cagcatcagc agcatgagca
     1321 gtgtcagctc ggtcctggat gagaaggacg atgaccggat ccgctgctgt acacactgca
     1381 aggacacgct gctcaagaga gagcagcaga ttgatgagaa ggagcacaca cctgacatcg
     1441 tgaagctcta cgagaaatta cgactttgca tggagaaagt tgaccagaaa gctccagaat
     1501 acatcaggat ggcagcatca ttaaatgctg gggagacaac ctacagtctg gaacatgcca
     1561 gtgaccttcg agtggaagtg cagaaagtgt atgagctgat agacgcttta agtaagaaga
     1621 tcttaacctt gggcttgaac caggaccctc caccacatcc aagcaatttg cggctgcaga
     1681 gaatgatcag atactcagct acactttttg tgcaggaaaa gttgcttggt ttgatgtcac
     1741 tgccaaccaa agaacagttt gaggaactga aaaagaaaag gaaggaggaa atggagagga
     1801 agagggccgt ggagagacaa gctgccctgg agtcccagcg aaggcttgag gaaaggcaga
     1861 gtggcctggc ttctcgagcg gccaacgggg aggtggcatc tctccgcagg ggccctgccc
     1921 ccttgagaaa ggctgagggc tggctcccac tgtcaggagg tcaggggcag agtgaggact
     1981 cagacccgct cctccagcag atccacaaca tcacatcatt catcaggcag gccaaggccg
     2041 cgggccgcat ggatgaagtg cgcactctgc aggagaacct gcggcagctg caggacgagt
     2101 atgaccagca gcagacagag aaggccatcg agctgtcccg gaggcaggct gaggaggagg
     2161 acctgcagcg ggaacagctg cagatgttgc gtgaacggga gttggaacga gaaagggagc
     2221 agtttcgggt ggcatccctg cacacacgga ctcggtccct ggacttcaga gaaatcggcc
     2281 cttttcagct ggagcccagc agagagcctc gcacccacct tgcttatgct ttggatctag
     2341 gctcttcccc agttccaagc agcacagctc ccaagacccc ttcacttagc tcaactcaac
     2401 ccaccagagt gtggtctggg cccccagccg ttggccagga gcgcttaccc cagagcagca
     2461 tgccacagca acatgagggg ccctccttaa acccctttga tgaggaagac ctctccagcc
     2521 ccatggaaga ggccactact ggtcctcctg ctgcaggggt ttccttagac ccttcagccc
     2581 gcatcctgaa agagtacaat cctttcgagg aagaggacga ggaggaggaa gcagtggcag
     2641 ggaatccatt cattcagcca gacagcccag ctcctaaccc cttcagtgag gaagacgaac
     2701 atccccagca gaggctctca agccctctgg ttcctggtaa cccctttgag gaacccacct
     2761 gtatcaaccc ctttgagatg gacagtgaca gtgggccaga ggctgaggag cccatagagg
     2821 aagagctcct cctgcagcag atcgataaca tcaaggcata catctttgat gccaagcagt
     2881 gcggccgcct ggatgaggta gaggtgctga cagagaatct gcgggagctg aagcacaccc
     2941 tggccaagca gaaggggggc actgactgac cagcagtgga gagggcacct ttgggcccag
     3001 gggtctggca ggagccagtg gagcaggaca gagggcaggc aggatggatg gggaaggtgg
     3061 cagggtgaga actcagatgc acacaggtga ggggcaggaa tctgctgttt tgtgttgcgc
     3121 actttgaggt atttccacta cagttgaata ataaaataga aactagaaca gggagaatca
     3181 gcattcagtt gctgcttttc ctgtttatta ttactatctt ttgtaatcgg aggtttaccc
     3241 cttttgaagg gactttacat ttttactacc gagatataac taaatgcagc tctgttgggc
     3301 ccagggcaga aatggctgct gtgtacctct tgggtccatt tgctactgcc tagtcttggt
     3361 tccttatgca gtattatagg gcagcctttt tagagccctt cctttagcca agacagagaa
     3421 gatagattcc actgagctct attctgctct gacagaagtc catccctagt aggctgtgag
     3481 ttccatttca cctggggccg cctctcccct gctctgcact tcctgtctgt acaatagaag
     3541 ggggaggtgc tgctatgaag gggagagttt agacccagga gagcccagca cctcttttta
     3601 aggtggggtg atgggaatat ttcaccaggg tctattttct cagtttaagt tcttttttgt
     3661 ctctttcagg aagttaagct cccagtgcag ggtatcaatg tgaatctggt cctgagcttt
     3721 ttagaaaata agagtggtgg ccgggcgcgc tggctcacgc ctgtaatcgc aacactttgg
     3781 gaggccgagg caggcagatc acaaggtcag gagatcgaga ccaccctggc taacacagtg
     3841 aaatcccgtc tctactaaaa atacaaaaaa ttagccaggc gtggtggtgg gtgcctgtag
     3901 tcccagctac tttggaggct gaggcaggag aatgctgtga acccgggagg cagagcttgc
     3961 agtgagccga gatcgcacca ctgcactcca gcctgggcag tgagagtgag actccgtctc
     4021 aaaaaaaaat aagagtggtt aactttttgg ttgatattag tattatttgt gagaagagtt
     4081 tccccaccct tcttctgata gaacaattgt ctgtcactgg agaaatctcc ctccagagct
     4141 ttggcaaagt tactctgatc tgggtctgtt taaaaggccg ggtctctaat ttaggaatcg
     4201 gtgatttgga agcttttgca gaacatcacc agaagaaggg aagcttccca gagtcagaag
     4261 ctaaattaaa ataatttcca aggctatttg atcagccttc ttcctttttg gttcattgtg
     4321 tcctgacttg gggcactgat gagatttttt attttttttg agacagagtt tcactttgtt
     4381 gcccaggctg gagtgtagtg gcatgatctc agctcactgc aacctccacc tccctggttc
     4441 aagtgatatt tgtgcctcag ccccctgaat agctaggatt acaggcgtgg gccaccacgc
     4501 ctggttaatc tctgtatttt tagtagaggt agagtttcac cattttggcc aggctggttt
     4561 cgaactcctg gcctcaagtg atctgccacc ttggcctccc aaagtgctgg gaatacaggc
     4621 gtgagccact gtgcccgccc tgatgagata ttttattacc aatgttagta ttgagaaact
     4681 gaaatgtttg aagaagcaca acccaggatc gtgctggtag caccacagta cttaaactgt
     4741 tggtcaatta aggccagaaa gggaaattgt taatttagct ctggtgcttt ggtttacagg
     4801 aacataactc ttaactgaca tctgacatca tgatagccat atgtgctcag ctctgggtag
     4861 agtttctgca gttactcatc tgaactaatg aacaataact gaccactagt cactttatgc
     4921 cgtgtaacta gctctaggcc atactttcac tggttactgg tgtgaaagct gaaattcatt
     4981 ttgttacatt ctggtgaaga cccctcttga taatgggaat gttttaactc tcttgatgaa
     5041 aaaataatct gtatttgtgt tgatgttcac atttctgtag cacatttctt atccttttgg
     5101 ttgaatgaaa agatcttgta taggggtgtg gagatgggga gtgggtagaa gtgtgtgaag
     5161 gacgctttgc atttgggatc tgttcacaaa cagccatatg agtgtgttaa tgaatgtcag
     5221 ccagttacca accctgctgg ttgttatggg ttgttttgag aagttggcaa ccaggcatct
     5281 aagatgttgc ctggtacagg cctcttttct tccctgaggc ccatgacatt tctctgttac
     5341 tctagagggt ttctcagatg gccagtaggc tcctccctgc ttagggtctc atttctctga
     5401 aaagaggatg aactgaaaag caggtagttc cagaagctaa ttgctgcttt ccatcataat
     5461 tatttttctt gtgagaacat ttctctttta attagctagt gattttgatt aagactaatt
     5521 cactaaacat acccttccct caaatcacct caggtagcaa tctgtaagta actaaaagca
     5581 ttgaaaaaca cacaagaaac atttttaaaa actattttta aaggcctggc cgggtgcagt
     5641 ggctcactcc tgtaatccca gcactttggg aggccgagac gggtggatca cctgaggtca
     5701 ggaatttgag actagcatgg ccaacatggt gaaaccccgt gtctactaaa aatacaaaaa
     5761 ttagctgggc gtggtggcag acgcctgtaa tcccagctac tcagggaggc tgaggcacga
     5821 gaatcgcttg aacctgggag gcggaggttg cagtgagcca agattgcgcc actgcactcc
     5881 aacctgggtg acaagagcaa gactccgtct cacaaaaaac aaacaaaact gactgattga
     5941 atatactaaa ccaaactaaa atcatattct tttgattaag tttatccatg gctgtattct
     6001 tctatgaatt cttccattta attcttccac gattatcttc tcctgtaaat acatcacagg
     6061 agttagaatt ctctacccat cagctgtacc atgtcgcaga attcatgcag gcacaaagtt
     6121 ggagttacag agatgggttg acagcaggca aacttggcct atgtattata accacaactt
     6181 caagttctta cctcatgtga atattcaccc tttctttagt cttccaaggc aaacagcccc
     6241 gtctcatcac cagatgagca aggtcttgat atggcatagc agatctccct agacacagat
     6301 catgagaaaa gatggaagag acttagggat tcaggcatca gatgaagttg gcttttccct
     6361 tttatgcctt gtttgtattt accctgtcta atacactaag gatacttact cattgtactt
     6421 gcagctcaat atgtctttgc tgttcagata ctaaaatgta cctctgagtc attgtgagct
     6481 gtgtggtagg ttggacattg gcatagttgg tgatgggact caaaatgaaa aggtggtctc
     6541 tttaccaggt cacagactgt agcagattgt gcttgttatc tgacaatgac tgtcactttg
     6601 agggtcgttg atttgcatgc actactctgg ggccttgtat tggagccttt tttaaaaaaa
     6661 ataaaatctg agatagag
//
