LOCUS       NM_032905               3275 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens RNA binding motif protein 17 (RBM17), transcript
            variant 1, mRNA.
ACCESSION   NM_032905 NM_006450
VERSION     NM_032905.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Fukumura,K., Sperotto,L., Seuss,S., Kang,H.S., Yoshimoto,R.,
            Sattler,M. and Mayeda,A.
  TITLE     SAP30BP interacts with RBM17/SPF45 to promote splicing in a subset
            of human short introns
  JOURNAL   Cell Rep 42 (12), 113534 (2023)
   PUBMED   38065098
  REMARK    GeneRIF: SAP30BP interacts with RBM17/SPF45 to promote splicing in
            a subset of human short introns.
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Kliche,J., Garvanska,D.H., Simonetti,L., Badgujar,D.,
            Dobritzsch,D., Nilsson,J., Davey,N.E. and Ivarsson,Y.
  TITLE     Large-scale phosphomimetic screening identifies phospho-modulated
            motif-based protein interactions
  JOURNAL   Mol Syst Biol 19 (7), e11164 (2023)
   PUBMED   37219487
REFERENCE   3  (bases 1 to 3275)
  AUTHORS   Fu,Y., Bai,C., Wang,S., Chen,D., Zhang,P., Wei,H., Rong,F.,
            Zhang,C., Chen,S. and Wang,Z.
  TITLE     AKT1 phosphorylates RBM17 to promote Sox2 transcription by
            modulating alternative splicing of FOXM1 to enhance cancer stem
            cell properties in colorectal cancer cells
  JOURNAL   FASEB J 37 (1), e22707 (2023)
   PUBMED   36520054
  REMARK    GeneRIF: AKT1 phosphorylates RBM17 to promote Sox2 transcription by
            modulating alternative splicing of FOXM1 to enhance cancer stem
            cell properties in colorectal cancer cells.
REFERENCE   4  (bases 1 to 3275)
  AUTHORS   Liu,L., Vujovic,A., Deshpande,N.P., Sathe,S., Anande,G.,
            Chen,H.T.T., Xu,J., Minden,M.D., Yeo,G.W., Unnikrishnan,A.,
            Hope,K.J. and Lu,Y.
  TITLE     The splicing factor RBM17 drives leukemic stem cell maintenance by
            evading nonsense-mediated decay of pro-leukemic factors
  JOURNAL   Nat Commun 13 (1), 3833 (2022)
   PUBMED   35781533
  REMARK    GeneRIF: The splicing factor RBM17 drives leukemic stem cell
            maintenance by evading nonsense-mediated decay of pro-leukemic
            factors.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3275)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 3275)
  AUTHORS   Perry,W.L. 3rd, Shepard,R.L., Sampath,J., Yaden,B., Chin,W.W.,
            Iversen,P.W., Jin,S., Lesoon,A., O'Brien,K.A., Peek,V.L., Rolfe,M.,
            Shyjan,A., Tighe,M., Williamson,M., Krishnan,V., Moore,R.E. and
            Dantzig,A.H.
  TITLE     Human splicing factor SPF45 (RBM17) confers broad multidrug
            resistance to anticancer drugs when overexpressed--a phenotype
            partially reversed by selective estrogen receptor modulators
  JOURNAL   Cancer Res 65 (15), 6593-6600 (2005)
   PUBMED   16061639
  REMARK    GeneRIF: Resistance to various chemotherapeutics resulting from
            stable transfection of RBM17 (SPF45) in A2780 ovarian carcinoma
            cells may be due to effects of SPF45 on transcription and splicing
            of ERbeta-regulated genes.
REFERENCE   7  (bases 1 to 3275)
  AUTHORS   Sampath,J., Long,P.R., Shepard,R.L., Xia,X., Devanarayan,V.,
            Sandusky,G.E., Perry,W.L. 3rd, Dantzig,A.H., Williamson,M.,
            Rolfe,M. and Moore,R.E.
  TITLE     Human SPF45, a splicing factor, has limited expression in normal
            tissues, is overexpressed in many tumors, and can confer a
            multidrug-resistant phenotype to cells
  JOURNAL   Am J Pathol 163 (5), 1781-1790 (2003)
   PUBMED   14578179
  REMARK    GeneRIF: overexpression of SPF45 in HeLa, a cervical carcinoma cell
            line, resulted in drug resistance to doxorubicin and vincristine
REFERENCE   8  (bases 1 to 3275)
  AUTHORS   Graveley,B.R.
  TITLE     Sex, AGility, and the regulation of alternative splicing
  JOURNAL   Cell 109 (4), 409-412 (2002)
   PUBMED   12086596
  REMARK    Review article
REFERENCE   9  (bases 1 to 3275)
  AUTHORS   Lallena,M.J., Chalmers,K.J., Llamazares,S., Lamond,A.I. and
            Valcarcel,J.
  TITLE     Splicing regulation at the second catalytic step by Sex-lethal
            involves 3' splice site recognition by SPF45
  JOURNAL   Cell 109 (3), 285-296 (2002)
   PUBMED   12015979
REFERENCE   10 (bases 1 to 3275)
  AUTHORS   Neubauer,G., King,A., Rappsilber,J., Calvio,C., Watson,M., Ajuh,P.,
            Sleeman,J., Lamond,A. and Mann,M.
  TITLE     Mass spectrometry and EST-database searching allows
            characterization of the multi-protein spliceosome complex
  JOURNAL   Nat Genet 20 (1), 46-50 (1998)
   PUBMED   9731529
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CV570433.1, AF542550.1,
            CN291330.1, AL157395.17 and AA905075.1.
            
            On Nov 22, 2018 this sequence version replaced NM_032905.4.
            
            Summary: This gene encodes an RNA binding protein. The encoded
            protein is part of the spliceosome complex and functions in the
            second catalytic step of mRNA splicing. Alternatively spliced
            transcript variants have been described. Related pseudogenes exist
            on chromosomes 9 and 15. [provided by RefSeq, Mar 2009].
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Both variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.162318.1, AF542550.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000379888.9/ ENSP00000369218.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-10                CV570433.1         49-58
            11-2708             AF542550.1         1-2698
            2709-2904           CN291330.1         329-524
            2905-3071           AL157395.17        61172-61338
            3072-3275           AA905075.1         20-223              c
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10p15.1"
     gene            1..3275
                     /gene="RBM17"
                     /gene_synonym="SPF45"
                     /note="RNA binding motif protein 17"
                     /db_xref="GeneID:84991"
                     /db_xref="HGNC:HGNC:16944"
                     /db_xref="MIM:606935"
     exon            1..160
                     /gene="RBM17"
                     /gene_synonym="SPF45"
                     /inference="alignment:Splign:2.1.0"
     exon            161..301
                     /gene="RBM17"
                     /gene_synonym="SPF45"
                     /inference="alignment:Splign:2.1.0"
     CDS             179..1384
                     /gene="RBM17"
                     /gene_synonym="SPF45"
                     /note="splicing factor 45kDa; 45 kDa-splicing factor"
                     /codon_start=1
                     /product="splicing factor 45"
                     /protein_id="NP_116294.1"
                     /db_xref="CCDS:CCDS7077.1"
                     /db_xref="GeneID:84991"
                     /db_xref="HGNC:HGNC:16944"
                     /db_xref="MIM:606935"
                     /translation="MSLYDDLGVETSDSKTEGWSKNFKLLQSQLQVKKAALTQAKSQR
                     TKQSTVLAPVIDLKRGGSSDDRQIVDTPPHVAAGLKDPVPSGFSAGEVLIPLADEYDP
                     MFPNDYEKVVKRQREERQRQRELERQKEIEEREKRRKDRHEASGFARRPDPDSDEDED
                     YERERRKRSMGGAAIAPPTSLVEKDKELPRDFPYEEDSRPRSQSSKAAIPPPVYEEQD
                     RPRSPTGPSNSFLANMGGTVAHKIMQKYGFREGQGLGKHEQGLSTALSVEKTSKRGGK
                     IIVGDATEKDASKKSDSNPLTEILKCPTKVVLLRNMVGAGEVDEDLEVETKEECEKYG
                     KVGKCVIFEIPGAPDDEAVRIFLEFERVESAIKAVVDLNGRYFGGRVVKACFYNLDKF
                     RVLDLAEQV"
     exon            302..418
                     /gene="RBM17"
                     /gene_synonym="SPF45"
                     /inference="alignment:Splign:2.1.0"
     exon            419..585
                     /gene="RBM17"
                     /gene_synonym="SPF45"
                     /inference="alignment:Splign:2.1.0"
     exon            586..683
                     /gene="RBM17"
                     /gene_synonym="SPF45"
                     /inference="alignment:Splign:2.1.0"
     exon            684..740
                     /gene="RBM17"
                     /gene_synonym="SPF45"
                     /inference="alignment:Splign:2.1.0"
     exon            741..882
                     /gene="RBM17"
                     /gene_synonym="SPF45"
                     /inference="alignment:Splign:2.1.0"
     exon            883..1034
                     /gene="RBM17"
                     /gene_synonym="SPF45"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1108
                     /gene="RBM17"
                     /gene_synonym="SPF45"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1207
                     /gene="RBM17"
                     /gene_synonym="SPF45"
                     /inference="alignment:Splign:2.1.0"
     exon            1208..1280
                     /gene="RBM17"
                     /gene_synonym="SPF45"
                     /inference="alignment:Splign:2.1.0"
     exon            1281..3275
                     /gene="RBM17"
                     /gene_synonym="SPF45"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1557..1562
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RBM17"
                     /gene_synonym="SPF45"
                     /note="hexamer: TATAAA"
     polyA_site      1581
                     /gene="RBM17"
                     /gene_synonym="SPF45"
                     /note="major polyA site"
     regulatory      2641..2646
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RBM17"
                     /gene_synonym="SPF45"
                     /note="hexamer: AATAAA"
     polyA_site      2660
                     /gene="RBM17"
                     /gene_synonym="SPF45"
     regulatory      2684..2689
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RBM17"
                     /gene_synonym="SPF45"
                     /note="hexamer: AATAAA"
     polyA_site      2708
                     /gene="RBM17"
                     /gene_synonym="SPF45"
     regulatory      3253..3258
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RBM17"
                     /gene_synonym="SPF45"
                     /note="hexamer: ATTAAA"
     polyA_site      3275
                     /gene="RBM17"
                     /gene_synonym="SPF45"
ORIGIN      
        1 agcccgcgct ccctgacccc gcgccgccga gcgccctgag gactcagcga agggtgggcg
       61 ccgccgaggc ctcctgccgc tggcgggttt ccgcggagtg ccgcccggct ccgctctgcc
      121 gccggcgcgg ctcatgggca gagtcggccg ggcgggccgg cattaaactg aagaaaagat
      181 gtccctgtac gatgacctag gagtggagac cagtgactca aaaacagaag gctggtccaa
      241 aaacttcaaa cttctgcagt ctcagcttca ggtgaagaag gcagctctca ctcaggcaaa
      301 gagccaaagg acgaaacaaa gtacagtcct cgccccagtc attgacctga agcgaggtgg
      361 ctcctcagat gaccggcaaa ttgtggacac tccaccgcat gtagcagctg ggctgaagga
      421 tcctgttccc agtgggtttt ctgcagggga agttctgatt cccttagctg acgaatatga
      481 ccctatgttt cctaatgatt atgagaaagt agtgaagcgc caaagagagg aacgacagag
      541 acagcgggag ctggaaagac aaaaggaaat agaagaaagg gaaaaaaggc gtaaagacag
      601 acatgaagca agtgggtttg caaggagacc agatccagat tctgatgaag atgaagatta
      661 tgagcgagag aggaggaaaa gaagtatggg cggagctgcc attgccccac ccacttctct
      721 ggtagagaaa gacaaagagt taccccgaga ttttccttat gaagaggact caagacctcg
      781 atcacagtct tccaaagcag ccattcctcc cccagtgtac gaggaacaag acagaccgag
      841 atctccaacc ggacctagca actccttcct cgctaacatg gggggcacgg tggcgcacaa
      901 gatcatgcag aagtacggct tccgggaggg ccagggtctg gggaagcatg agcagggcct
      961 gagcactgcc ttgtcagtgg agaagaccag caagcgtggc ggcaagatca tcgtgggcga
     1021 cgccacagag aaagatgcat ccaagaagtc agattcaaat ccgctgactg aaatacttaa
     1081 gtgtcctact aaagtggtct tactaaggaa catggttggt gcgggagagg tggatgaaga
     1141 cttggaagtt gaaaccaagg aagaatgtga aaaatatggc aaagttggaa aatgtgtgat
     1201 atttgaaatt cctggtgccc ctgatgatga agcagtacgg atatttttag aatttgagag
     1261 agttgaatca gcaattaaag cggttgttga cttgaatggg aggtattttg gtggacgggt
     1321 ggtaaaagca tgtttctaca atttggacaa attcagggtc ttggatttgg cagaacaagt
     1381 ttgattttaa gaactagagc acgagtcatc tccggtgatc cttaaatgaa ctgcaggctg
     1441 agaaaagaag gaaaaaggtc acagcctcca tggctgttgc ataccaagac tcttggaagg
     1501 acttctaaga tatatgttga ttgatccctt ttttattttg tggtttttta atatagtata
     1561 aaaatccttt taaaaaaaca acaatctgtg tgcctctctg gttgtttctc ttttttatta
     1621 ttactcctga gttgatgaca ttttttgtta gatttcatgg taattctcaa gtgcttcaat
     1681 gatgcagcat ttcttgcact aaaaaaaaaa aaaaaaaaaa aactagaaag ttttgggaca
     1741 tggggttata ttaaattatt ctttgttttt ctttttcttt taataaagcc tgcaagttac
     1801 taaattgtag tttcataaat tctgtagtaa agtatcatct tggcagtgtg ccaaaggtga
     1861 aaatgatgct ttctctaaca gagaaattct tagtgactcc agtcgtagaa aaacgtcttt
     1921 acaacctgaa taagattgaa gaattgtgaa cataccatgg cctattggat gaatcatttg
     1981 ccgtaggcta aatcagactg tagggtttgt gatggattta tggagtatgt gggtatagaa
     2041 atcatgaatc tagcatttgt tttcagagat tcaagcatag tcttaagggt agatcagaaa
     2101 tgacaaatga attcaaaacc tagcaggtgc attgtaaatg tgtgcccagt tatgttttgg
     2161 aaatggcagt tccttggggt catgtttcta ctggcaaaat ttgcaatagt gttctattgt
     2221 atgtaatttt aaaatttata agattatcca cgttggccaa gtaaactgta ctgccaatag
     2281 aattctggaa ttgtgagaaa ttgtatcatt gaagttcagt aggatgtgtg gcttaaaaat
     2341 ttatcaggac cacaaaaaag aaaacaaaaa tatttggtac tgaggttcat tgccagggca
     2401 ggaggtattt ccagaaaata ctcatgcctg tgttctgttc cttgctttcc caaatactgc
     2461 atgtgacttt cctaagcggc agctgaaaga ctcgagcccg tgctgtctcc tttggttatt
     2521 atgacatgaa agtgtatcaa gaactcagca tttctttgca tccatggact tggtttggag
     2581 acataaggaa tattctgacc ctttttaaaa aaggattttc tcatgttttt atttaacata
     2641 aataaaagaa taacatttta tcttttgtgg tattatttta ttgaataaaa ttgagtttta
     2701 tgataaaagt gcacctgttc tgtaaagtaa gttggggtta aggaatgtga ggtaaattac
     2761 ataattgaac attgtggaag acagaatcaa aagcagccct ggaacttcag ttatctatgg
     2821 aattaggcat accattcctc aagtggaaac catgtttgtg cttacaagta ccggagtgtg
     2881 cttgtttaat tttagaaact aatggttcca acccaccttt catgcatgca tttattgttg
     2941 ttatgctttg taaaacattg tttgcaccta aatgggtggc ttttcctaac attctcatgg
     3001 tcagcaacca gagagttgca accaactcat actacttgat ttccgttcgc atgaggacag
     3061 cttggtgtgt gcctttctcc ccagtctttt atttttaaaa taactgtgtt aatcagttag
     3121 tgctttattt ataatgaatt tctgatagtc gaataatttc taaatctcct gcaagttagt
     3181 acttgagaaa tttgaaatta attttcaata ttaacattta agctaatata aaaattttaa
     3241 atttcaataa aaattaaaaa ttatgtaagc tacaa
//
