LOCUS       NM_001394102            5654 bp    mRNA    linear   PRI 22-SEP-2024
DEFINITION  Homo sapiens Ras association domain family member 8 (RASSF8),
            transcript variant 13, mRNA.
ACCESSION   NM_001394102
VERSION     NM_001394102.1
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5654)
  AUTHORS   Lin,Y., Guo,R., R,G. and Xu,B.
  TITLE     Tailored modulation of S100A1 and RASSF8 expression by
            butanediamide augments healing of rotator cuff tears
  JOURNAL   PeerJ 11, e15791 (2023)
   PUBMED   37601265
  REMARK    GeneRIF: Tailored modulation of S100A1 and RASSF8 expression by
            butanediamide augments healing of rotator cuff tears.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5654)
  AUTHORS   Li,X., Jiang,X., Lu,J., Lin,Y., Jiang,L., Li,Y., Wan,F. and Wang,C.
  TITLE     CircCERS6 Suppresses the Development of Epithelial Ovarian Cancer
            Through Mediating miR-630/RASSF8
  JOURNAL   Biochem Genet 60 (6), 2611-2629 (2022)
   PUBMED   35676548
  REMARK    GeneRIF: CircCERS6 Suppresses the Development of Epithelial Ovarian
            Cancer Through Mediating miR-630/RASSF8.
REFERENCE   3  (bases 1 to 5654)
  AUTHORS   Zhang,X., Sun,P., Yang,W., Li,S., Jia,H., Wang,W., Jiang,S.,
            Wang,L., Liu,X., Liang,L. and Zi,W.
  TITLE     RASSF8-AS1 displays low expression in colorectal cancer and
            up-regulates RASSF8 to suppress cell invasion and migration
  JOURNAL   Pathol Res Pract 237, 153996 (2022)
   PUBMED   35839610
  REMARK    GeneRIF: RASSF8-AS1 displays low expression in colorectal cancer
            and up-regulates RASSF8 to suppress cell invasion and migration.
REFERENCE   4  (bases 1 to 5654)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 5654)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 5654)
  AUTHORS   Falvella,F.S., Manenti,G., Spinola,M., Pignatiello,C., Conti,B.,
            Pastorino,U. and Dragani,T.A.
  TITLE     Identification of RASSF8 as a candidate lung tumor suppressor gene
  JOURNAL   Oncogene 25 (28), 3934-3938 (2006)
   PUBMED   16462760
  REMARK    GeneRIF: Expression of RASSF8 protein by transfected lung cancer
            cells led to inhibition of anchorage-independent growth in soft
            agar in A549 cells and reduction of clonogenic activity in NCI-H520
            cells
REFERENCE   7  (bases 1 to 5654)
  AUTHORS   Jin,J., Smith,F.D., Stark,C., Wells,C.D., Fawcett,J.P.,
            Kulkarni,S., Metalnikov,P., O'Donnell,P., Taylor,P., Taylor,L.,
            Zougman,A., Woodgett,J.R., Langeberg,L.K., Scott,J.D. and Pawson,T.
  TITLE     Proteomic, functional, and domain-based analysis of in vivo 14-3-3
            binding proteins involved in cytoskeletal regulation and cellular
            organization
  JOURNAL   Curr Biol 14 (16), 1436-1450 (2004)
   PUBMED   15324660
REFERENCE   8  (bases 1 to 5654)
  AUTHORS   Debeer,P., Schoenmakers,E.F., Twal,W.O., Argraves,W.S., De Smet,L.,
            Fryns,J.P. and Van De Ven,W.J.
  TITLE     The fibulin-1 gene (FBLN1) is disrupted in a t(12;22) associated
            with a complex type of synpolydactyly
  JOURNAL   J Med Genet 39 (2), 98-104 (2002)
   PUBMED   11836357
REFERENCE   9  (bases 1 to 5654)
  AUTHORS   Gonzalez,P., Epstein,D.L. and Borras,T.
  TITLE     Characterization of gene expression in human trabecular meshwork
            using single-pass sequencing of 1060 clones
  JOURNAL   Invest Ophthalmol Vis Sci 41 (12), 3678-3693 (2000)
   PUBMED   11053263
REFERENCE   10 (bases 1 to 5654)
  AUTHORS   Debeer,P., Schoenmakers,E.F., Thoelen,R., Holvoet,M., Kuittinen,T.,
            Fabry,G., Fryns,J.P., Goodman,F.R. and Van de Ven,W.J.
  TITLE     Physical map of a 1.5 mb region on 12p11.2 harbouring a
            synpolydactyly associated chromosomal breakpoint
  JOURNAL   Eur J Hum Genet 8 (8), 561-570 (2000)
   PUBMED   10951517
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC055707.33.
            
            Summary: This gene encodes a member of the Ras-assocation domain
            family (RASSF) of tumor suppressor proteins. This gene is essential
            for maintaining adherens junction function in epithelial cells and
            has a role in epithelial cell migration. It is a lung tumor
            suppressor gene candidate. A chromosomal translocation
            t(12;22)(p11.2;q13.3) leading to the fusion of this gene and the
            FBLN1 gene is found in a complex type of synpolydactyly. Multiple
            alternatively spliced transcript variants encoding different
            isoforms have been found for this gene. [provided by RefSeq, May
            2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.66784.1,
                                           SRR1803615.99363.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN03465402 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-278               AC055707.33        41002-41279         c
            279-446             AC055707.33        24734-24901         c
            447-1336            AC055707.33        14793-15682         c
            1337-1481           AC055707.33        12467-12611         c
            1482-5654           AC055707.33        7311-11483          c
FEATURES             Location/Qualifiers
     source          1..5654
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p12.1"
     gene            1..5654
                     /gene="RASSF8"
                     /gene_synonym="C12orf2; HOJ1"
                     /note="Ras association domain family member 8"
                     /db_xref="GeneID:11228"
                     /db_xref="HGNC:HGNC:13232"
                     /db_xref="MIM:608231"
     exon            1..278
                     /gene="RASSF8"
                     /gene_synonym="C12orf2; HOJ1"
                     /inference="alignment:Splign:2.1.0"
     exon            279..446
                     /gene="RASSF8"
                     /gene_synonym="C12orf2; HOJ1"
                     /inference="alignment:Splign:2.1.0"
     CDS             344..1603
                     /gene="RASSF8"
                     /gene_synonym="C12orf2; HOJ1"
                     /note="isoform a is encoded by transcript variant 13; ras
                     association domain-containing protein 8; Ras association
                     (RalGDS/AF-6) domain family (N-terminal) member 8;
                     carcinoma-associated protein HOJ-1"
                     /codon_start=1
                     /product="ras association domain-containing protein 8
                     isoform a"
                     /protein_id="NP_001381031.1"
                     /db_xref="CCDS:CCDS53765.1"
                     /db_xref="GeneID:11228"
                     /db_xref="HGNC:HGNC:13232"
                     /db_xref="MIM:608231"
                     /translation="MELKVWVDGVQRIVCGVTEVTTCQEVVIALAQAIGRTGRYTLIE
                     KWRDTERHLAPHENPIISLNKWGQYASDVQLILRRTGPSLSERPTSDSVARIPERTLY
                     RQSLPPLAKLRPQIDKSIKRREPKRKSLTFTGGAKGLMDIFGKGKETEFKQKVLNNCK
                     TTADELKKLIRLQTEKLQSIEKQLESNEIEIRFWEQKYNSNLEEEIVRLEQKIKRNDV
                     EIEEEEFWENELQIEQENEKQLKDQLQEIRQKITECENKLKDYLAQIRTMESGLEAEK
                     LQREVQEAQVNEEEVKGKIGKVKGEIDIQGQQSLRLENGIKAVERSLGQATKRLQDKE
                     QELEQLTKELRQVNLQQFIQQTGTKVTVLPAEPIEIEASHADIEREAPFQSGSLKRPG
                     SSRQLPSNLRILQNPISSGFNPEGIYV"
     exon            447..1336
                     /gene="RASSF8"
                     /gene_synonym="C12orf2; HOJ1"
                     /inference="alignment:Splign:2.1.0"
     exon            1337..1481
                     /gene="RASSF8"
                     /gene_synonym="C12orf2; HOJ1"
                     /inference="alignment:Splign:2.1.0"
     exon            1482..5654
                     /gene="RASSF8"
                     /gene_synonym="C12orf2; HOJ1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5628..5633
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RASSF8"
                     /gene_synonym="C12orf2; HOJ1"
                     /note="hexamer: AATAAA"
     polyA_site      5654
                     /gene="RASSF8"
                     /gene_synonym="C12orf2; HOJ1"
                     /note="major polyA site"
ORIGIN      
        1 acagatattt attctttcta cataagtgta ggactttttc ccttattttt ctagaaaatg
       61 tttatatttc taaagtttgc ttttgaattc tttttttaaa aagctatttt aattgtttca
      121 cagtgtgaag cattagaatc acagaactgt aaggaacatc atggatgttc cttgtgattg
      181 ggaaaggaat gatccacggc agtgagtttc attggggatc ctactcctat gaaatttggc
      241 atactgctca ggtcaggttc agaaggtgaa ttcttaagct agagacatga cctaacaccc
      301 tgatgaccac tctcagggac cttgagtgac tggccggtgc accatggaac ttaaagtatg
      361 ggtggatgga gttcagagga ttgtttgtgg agtcactgaa gtcacaactt gccaggaggt
      421 tgtcatagcc ttagctcaag caataggtcg aactggaagg tacaccctta tagagaaatg
      481 gagagatact gaaagacact tagcacctca tgaaaatcct atcatatcct taaacaaatg
      541 ggggcagtat gctagtgatg tgcagctcat tctacgacga actgggccgt ctctcagtga
      601 gcgacccact tcagacagtg tggctcgaat tcctgaaaga actttataca ggcagagtct
      661 gcccccctta gctaaactga ggcctcagat tgacaaatca atcaaaagga gggaaccgaa
      721 aaggaaatca ctgacattta caggaggtgc caaaggatta atggacattt ttggaaaagg
      781 taaagaaact gagtttaagc aaaaggtgct gaataactgc aaaacaacag cagatgagtt
      841 gaagaagcta atccgtctgc agacagagaa gcttcaatcc attgagaaac agctggaatc
      901 taatgaaata gaaataagat tttgggagca aaagtataat tccaaccttg aagaggaaat
      961 tgtccgtcta gagcaaaaga tcaaaagaaa cgatgtagaa attgaggagg aagaattctg
     1021 ggaaaatgaa ttacagattg aacaggaaaa tgaaaaacag ctgaaggatc aacttcaaga
     1081 aataagacag aaaataacag aatgtgaaaa caaattaaag gactatttgg cacagatccg
     1141 gactatggaa agtggtcttg aagcagaaaa attgcaacgg gaagttcaag aggcacaggt
     1201 caatgaggaa gaggttaaag gaaagatcgg taaggtcaaa ggggagattg acattcaagg
     1261 ccagcagagt ctgaggttgg aaaatggcat caaagctgtg gaaagatctc ttggacaagc
     1321 caccaaacgc ttacaggaca aagaacagga actggagcag ttgactaagg agttgcggca
     1381 agtcaatctc cagcagttca tccagcagac agggacaaaa gttaccgttt tgccagcgga
     1441 gcccattgaa atagaggcct cacatgcaga cattgaaagg gaggcaccat tccagtctgg
     1501 gtccctgaag cgacctggtt catctcggca gctccccagt aatctccgca ttctgcagaa
     1561 tcctatctca tctggtttta atcctgaagg catatatgta tgacattatc tgtctttagg
     1621 gaggagaccc aacagaggta ccaaggacag taaacttcct ttttgatttg tgccaatgat
     1681 gaacagagga tctattccac aagacgctgt atgttttttc tctcctaaat tgcataccac
     1741 ttggagccat accctgtgca ctgatgctaa agaacaaaga aactgtgttt tcacacatca
     1801 acagtgttga tatttttgtc cagcagctat aatgcaaagc cttcgttttt atttgtagca
     1861 ttttgagagc tttaggaaag tattatatag tgtgtataca taaataagcc gtgacttaag
     1921 taagagtgaa gagaaatttg tgactggctt agtttaattt atttcatgta atcaatgtgt
     1981 gaatgttgat gttttaatat ttttattgac ttatcctgtg actgacttac gttacatatt
     2041 gtgtgattat gaccgtgtgc atgttgccag actccatcca tgcattgctg atttacacta
     2101 cacaagtgtc ctagggtgct ccaccatcga agctaactcc acaggaagcc acttgcattt
     2161 gttttgtgaa actgtcctag ccattgctta attaggtgaa ataattcagg gttttttaaa
     2221 tctggaattt agtcgttcct ttacaatatt tccaaatata cgtgtggcca cagagcataa
     2281 cagatatttt tcataagcta aattgtatgt ataaaacatt tgcagtgttt cagacactgt
     2341 tgaacaaaaa tgtaattggt aagtatgtat ccaaacaggc atggggtttc ctgatacatt
     2401 atgtgttttg tttctgccct gtcttatcat ttacactcat ggatcttcag aattaatcta
     2461 acatggaagt tatagatacc tgaaagctgg gttggtctac ttcaggaaca ttgctttaag
     2521 tgatgtattt taaaataacc cgcttcatat gtatgatctg ttggtgtaca caccatgggt
     2581 aaggtattgc ttgcacataa tttgctctgc atattatgga ccattgtggt ttcttccagt
     2641 cacttagatg gaaaggaggt taaacctagg tactttttag caaggatata aagtcaaatt
     2701 cagcatatgt ttttattttt aggcttgact tttacaagac attaatctcc atttgtacat
     2761 atgttatttt atttgtaaaa ccaaatatga ctcaacacct ttttgatgag tagtgactta
     2821 actaagattt acaagtaata tttagacaga ttcagtcaga caccacttag ccatttttac
     2881 attccctctg gttagatttg gtacagtata attaagactt taatctgaaa tttaaagtag
     2941 ttttaattct gaagaagtta catcctctgt attatttaca tgcaacaaaa taaaagtgga
     3001 ttaatatagt aatgccatgg taacaataga gctatgtctt atgcatgaag gcaactttgg
     3061 atgttttaac acatttgact gactttggtt tagtgaatgc tgacaatgct gctctactta
     3121 ggtttcctat gaaattttta agaatggcta tgagactgta tagaagcttc tagagaactg
     3181 aaagtcataa tgcagagttg ccttttctag tgcagctaag tggcggacct caactgaaga
     3241 tatgagtttc tttgggttct tggagttatc aaattgatct tgccattatg ttactttgca
     3301 aataactgaa gtaaatgatt cttacctaaa taaccacaac ctgtacacat ttgctcacaa
     3361 tttatagtag ttattttcta tctacctata tttaaaatta ggatgattaa aaaataattt
     3421 atagattttg tgacagtaat gatttacata tgcccaatat atgccttatt tttaaataag
     3481 tcattctgta taaagtcatt ttgttgttgt tcagagaaat caagatctta ttcacaaaga
     3541 aaaacatttt ataaattgat cgcattttct ttaagtcaaa atgttaaact ggaggcagta
     3601 ttaaactttg caattaggag tttcagagat gccttggcat accacgaaaa acactgtcaa
     3661 tatccaactc attataaatt gttatcagaa ttaacctaat aactttaagt aaggacaagc
     3721 agcatcctag caacttgtag tagtcttggg ttcccagcag agtatcacag ttaacctctc
     3781 tgacaacttc atcagaattt ccaagctgcc aaataatctt catagaccta attacagatt
     3841 taaaaaaatt tcctaggcga cgaaagtata gaaatgtgaa tatgttgaat acattgagaa
     3901 gctgtacttt ttaattagtt atattacttc tggaagcaca tatctaagga atattttcta
     3961 agactcagag ggaaaaaaac tcgttttcat aggatcatct gaagatactt tagtatattt
     4021 gtgatcattt ttatctattg caaatacaaa tgaattagat aagtgccaca tcctggatac
     4081 atttcggagg ggtagtgggc aatggtatac aaaaatacca aatataaaat tttcatctgg
     4141 gggaatgttc aggttctaaa tactaaatta gatttcaatg ttttgtgttt tttttaaaaa
     4201 aatcatattg caacttgttt tattgtgatt ttctacaagt attaaataca tttaaagatc
     4261 tttttatctt accaagaaat aatttggaat agcattggta tatttgacct tttgagtgtc
     4321 tgtcatcctc agagaatggc ccatagtgtg ttgcctgtgg acatagtgtc catagtccct
     4381 ttcttgtcct tctgagatat tttggtttga taacattttg ttttttgtct tgatgcacag
     4441 ggactttttt ataatatgag acttcagttg gtattaatag gagttaccta tttaattctc
     4501 ccagtcatca atgaggtaat catcaattcc tatagttcaa attagtcata aacaagagct
     4561 acagcaagac tgatagagta aaaactatat aaatacagta aaaaaaaaat agaatttatg
     4621 gtgtgaaata tgaagtatag caatatataa caagaacatg taagcacttg cttccacagc
     4681 ataaatgtaa tttacatctg cattaaagga taattttctc tgtgaataag agcaaaatgg
     4741 tcttcagaga aacagactgt tcatttgaag ccatttctgt aaacatcttc caagatagaa
     4801 ctgtaatgga ttgaggaaat aacacagaaa agacaaaaac ttttgatttc aggcatgcaa
     4861 agtgcttggg cagatggaca gttcccattg tgcattgcct ttgataaatt tggccttaat
     4921 ttatataacg atgctgtgtt cacatccctc tcctacctaa agttgtcttt attggtttat
     4981 attgtgttaa aattagctta taattattac ttttgtattg tgttcagttt tttaagttgc
     5041 atgtttatac tatttgctac agtattttta agtttggggt gaaggccatc agctgtatca
     5101 aaaagacaaa acaatcacta tttattcagt attgctgctt tacatctcca gaataagctt
     5161 tcgatgccag gacagtgact gcctgaaagc tgcaggagct cttttcaatg cattttatat
     5221 atttttagaa accaaataaa tgcagaaaac tatttcgtat actaattata ttaaaccagc
     5281 agaaatataa aggcaataaa gtatatacat atatatgggg ggaggggaaa gattaaaaaa
     5341 tagatttaca gccagacatg gtgatagccc taaatgtatt tctcaatatg tttttcttta
     5401 ttgaccctag gaggatttga gttgctgcag ctttaaacag aaaattgcca tgttcactaa
     5461 ttgtgagcac ataaatatgc ttttagtact gctttgctta tgtacaaata aatctgtaat
     5521 atgtattata tgtaattatc cttttctttg actttcattt taaatgtata ttttctgatc
     5581 attatgagct gtaaaacaaa gaatcaatat tggatattct cccaaataat aaattttcag
     5641 gattcattgg ggca
//
