LOCUS       NP_060275                963 aa            linear   PRI 27-APR-2025
DEFINITION  ras-interacting protein 1 [Homo sapiens].
ACCESSION   NP_060275
VERSION     NP_060275.2
DBSOURCE    REFSEQ: accession NM_017805.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 963)
  AUTHORS   Xing,X., Wang,X., Liu,M., Guo,Q. and Wang,H.
  TITLE     Ras interacting protein 1 facilitated proliferation and invasion of
            diffuse large B-cell lymphoma cells
  JOURNAL   Cancer Biol Ther 24 (1), 2193114 (2023)
   PUBMED   36967521
  REMARK    GeneRIF: Ras interacting protein 1 facilitated proliferation and
            invasion of diffuse large B-cell lymphoma cells.
REFERENCE   2  (residues 1 to 963)
  AUTHORS   Rossi,T., Pistoni,M., Sancisi,V., Gobbi,G., Torricelli,F.,
            Donati,B., Ribisi,S., Gugnoni,M. and Ciarrocchi,A.
  TITLE     RAIN Is a Novel Enhancer-Associated lncRNA That Controls RUNX2
            Expression and Promotes Breast and Thyroid Cancer
  JOURNAL   Mol Cancer Res 18 (1), 140-152 (2020)
   PUBMED   31624086
  REMARK    GeneRIF: RAIN Is a Novel Enhancer-Associated lncRNA That Controls
            RUNX2 Expression and Promotes Breast and Thyroid Cancer.
REFERENCE   3  (residues 1 to 963)
  AUTHORS   Gingras,A.R., Puzon-McLaughlin,W., Bobkov,A.A. and Ginsberg,M.H.
  TITLE     Structural Basis of Dimeric Rasip1 RA Domain Recognition of the Ras
            Subfamily of GTP-Binding Proteins
  JOURNAL   Structure 24 (12), 2152-2162 (2016)
   PUBMED   27839947
  REMARK    GeneRIF: Results show that Rasip1 RA domain formed a dimer that can
            bind two Rap1 or Ras molecules and solved the Rap1-RRA complex and
            found that Rasip1 binds Rap1 in the Switch I region, and Rap1
            binding induces few conformation changes to Rasip1 stabilizing a
            beta strand and an unstructured loop.
REFERENCE   4  (residues 1 to 963)
  AUTHORS   de Kreuk,B.J., Gingras,A.R., Knight,J.D., Liu,J.J., Gingras,A.C.
            and Ginsberg,M.H.
  TITLE     Heart of glass anchors Rasip1 at endothelial cell-cell junctions to
            support vascular integrity
  JOURNAL   Elife 5, e11394 (2016)
   PUBMED   26780829
  REMARK    GeneRIF: These studies establish that the binding of HEG1 to Rasip1
            mediates Rap1-dependent recruitment of Rasip1 to and stabilization
            of endothelial cell cell-cell junctions.
            Publication Status: Online-Only
REFERENCE   5  (residues 1 to 963)
  AUTHORS   Keene,K.L., Chen,W.M., Chen,F., Williams,S.R., Elkhatib,S.D.,
            Hsu,F.C., Mychaleckyj,J.C., Doheny,K.F., Pugh,E.W., Ling,H.,
            Laurie,C.C., Gogarten,S.M., Madden,E.B., Worrall,B.B. and Sale,M.M.
  TITLE     Genetic Associations with Plasma B12, B6, and Folate Levels in an
            Ischemic Stroke Population from the Vitamin Intervention for Stroke
            Prevention (VISP) Trial
  JOURNAL   Front Public Health 2, 112 (2014)
   PUBMED   25147783
  REMARK    Publication Status: Online-Only
REFERENCE   6  (residues 1 to 963)
  AUTHORS   McKnight,N.C., Jefferies,H.B., Alemu,E.A., Saunders,R.E.,
            Howell,M., Johansen,T. and Tooze,S.A.
  TITLE     Genome-wide siRNA screen reveals amino acid starvation-induced
            autophagy requires SCOC and WAC
  JOURNAL   EMBO J 31 (8), 1931-1946 (2012)
   PUBMED   22354037
REFERENCE   7  (residues 1 to 963)
  CONSRTM   Psychiatric GWAS Consortium Bipolar Disorder Working Group
  TITLE     Large-scale genome-wide association analysis of bipolar disorder
            identifies a new susceptibility locus near ODZ4
  JOURNAL   Nat Genet 43 (10), 977-983 (2011)
   PUBMED   21926972
  REMARK    Erratum:[Nat Genet. 2012 Sep;44(9):1072. Fullerton, Janice M
            [added]; Hyoun, Phil L [corrected to Lee, Phil H]; Meng, Fan Guo
            [corrected to Meng, Fan]]
            Publication Status: Online-Only
REFERENCE   8  (residues 1 to 963)
  AUTHORS   Franke,A., McGovern,D.P., Barrett,J.C., Wang,K.,
            Radford-Smith,G.L., Ahmad,T., Lees,C.W., Balschun,T., Lee,J.,
            Roberts,R., Anderson,C.A., Bis,J.C., Bumpstead,S., Ellinghaus,D.,
            Festen,E.M., Georges,M., Green,T., Haritunians,T., Jostins,L.,
            Latiano,A., Mathew,C.G., Montgomery,G.W., Prescott,N.J.,
            Raychaudhuri,S., Rotter,J.I., Schumm,P., Sharma,Y., Simms,L.A.,
            Taylor,K.D., Whiteman,D., Wijmenga,C., Baldassano,R.N., Barclay,M.,
            Bayless,T.M., Brand,S., Buning,C., Cohen,A., Colombel,J.F.,
            Cottone,M., Stronati,L., Denson,T., De Vos,M., D'Inca,R.,
            Dubinsky,M., Edwards,C., Florin,T., Franchimont,D., Gearry,R.,
            Glas,J., Van Gossum,A., Guthery,S.L., Halfvarson,J.,
            Verspaget,H.W., Hugot,J.P., Karban,A., Laukens,D., Lawrance,I.,
            Lemann,M., Levine,A., Libioulle,C., Louis,E., Mowat,C., Newman,W.,
            Panes,J., Phillips,A., Proctor,D.D., Regueiro,M., Russell,R.,
            Rutgeerts,P., Sanderson,J., Sans,M., Seibold,F., Steinhart,A.H.,
            Stokkers,P.C., Torkvist,L., Kullak-Ublick,G., Wilson,D.,
            Walters,T., Targan,S.R., Brant,S.R., Rioux,J.D., D'Amato,M.,
            Weersma,R.K., Kugathasan,S., Griffiths,A.M., Mansfield,J.C.,
            Vermeire,S., Duerr,R.H., Silverberg,M.S., Satsangi,J.,
            Schreiber,S., Cho,J.H., Annese,V., Hakonarson,H., Daly,M.J. and
            Parkes,M.
  TITLE     Genome-wide meta-analysis increases to 71 the number of confirmed
            Crohn's disease susceptibility loci
  JOURNAL   Nat Genet 42 (12), 1118-1125 (2010)
   PUBMED   21102463
REFERENCE   9  (residues 1 to 963)
  AUTHORS   Ikram,M.K., Sim,X., Jensen,R.A., Cotch,M.F., Hewitt,A.W.,
            Ikram,M.A., Wang,J.J., Klein,R., Klein,B.E., Breteler,M.M.,
            Cheung,N., Liew,G., Mitchell,P., Uitterlinden,A.G., Rivadeneira,F.,
            Hofman,A., de Jong,P.T., van Duijn,C.M., Kao,L., Cheng,C.Y.,
            Smith,A.V., Glazer,N.L., Lumley,T., McKnight,B., Psaty,B.M.,
            Jonasson,F., Eiriksdottir,G., Aspelund,T., Harris,T.B.,
            Launer,L.J., Taylor,K.D., Li,X., Iyengar,S.K., Xi,Q.,
            Sivakumaran,T.A., Mackey,D.A., Macgregor,S., Martin,N.G.,
            Young,T.L., Bis,J.C., Wiggins,K.L., Heckbert,S.R., Hammond,C.J.,
            Andrew,T., Fahy,S., Attia,J., Holliday,E.G., Scott,R.J.,
            Islam,F.M., Rotter,J.I., McAuley,A.K., Boerwinkle,E., Tai,E.S.,
            Gudnason,V., Siscovick,D.S., Vingerling,J.R. and Wong,T.Y.
  CONSRTM   Global BPgen Consortium
  TITLE     Four novel Loci (19q13, 6q24, 12q24, and 5q14) influence the
            microcirculation in vivo
  JOURNAL   PLoS Genet 6 (10), e1001184 (2010)
   PUBMED   21060863
  REMARK    GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
            Erratum:[PLoS Genet. 2010;6(11). doi:
            10.1371/annotation/841bfadf-85d1-4059-894f-2863d73fa963. Xueling,
            Sim [corrected to Sim, Xueling]]
            Publication Status: Online-Only
REFERENCE   10 (residues 1 to 963)
  AUTHORS   Mitin,N.Y., Ramocki,M.B., Zullo,A.J., Der,C.J., Konieczny,S.F. and
            Taparowsky,E.J.
  TITLE     Identification and characterization of rain, a novel
            Ras-interacting protein with a unique subcellular localization
  JOURNAL   J Biol Chem 279 (21), 22353-22361 (2004)
   PUBMED   15031288
  REMARK    GeneRIF: Rain is a novel protein that can serve as an effector of
            endomembrane-localized Ras
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC042111.1, BC028614.1 and BC021860.1.
            
            On Dec 1, 2003 this sequence version replaced NP_060275.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC028614.1, AY378097.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000222145.9/ ENSP00000222145.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.33"
     Protein         1..963
                     /product="ras-interacting protein 1"
                     /calculated_mol_wt=103327
     Region          1..22
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q5U651.1)"
     Region          35..118
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q5U651.1)"
     Site            94
                     /site_type="methylation"
                     /note="Omega-N-methylarginine.
                     /evidence=ECO:0000250|UniProtKB:Q3U0S6; propagated from
                     UniProtKB/Swiss-Prot (Q5U651.1)"
     Region          134..256
                     /region_name="RA_Radil_like"
                     /note="Ras-associating (RA) domain found in
                     ras-associating and dilute domain-containing protein
                     (Radil) and similar proteins; cd17116"
                     /db_xref="CDD:340636"
     Site            order(147,149,156..165,181..182,206,208,225,227,232..234,
                     237,240..246,248,250)
                     /site_type="other"
                     /note="RA-Rap1b interaction site [polypeptide binding]"
                     /db_xref="CDD:340636"
     Site            188
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:24275569; propagated from
                     UniProtKB/Swiss-Prot (Q5U651.1)"
     Region          267..356
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q5U651.1)"
     Site            280
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q3U0S6; propagated from
                     UniProtKB/Swiss-Prot (Q5U651.1)"
     Site            292
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q3U0S6; propagated from
                     UniProtKB/Swiss-Prot (Q5U651.1)"
     Site            326
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q3U0S6; propagated from
                     UniProtKB/Swiss-Prot (Q5U651.1)"
     Site            328
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q3U0S6; propagated from
                     UniProtKB/Swiss-Prot (Q5U651.1)"
     Site            331
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q3U0S6; propagated from
                     UniProtKB/Swiss-Prot (Q5U651.1)"
     Region          378..490
                     /region_name="FHA_RAIN"
                     /note="forkhead associated (FHA) domain found in
                     Ras-interacting protein 1 (Rain); cd22734"
                     /db_xref="CDD:438786"
     Site            419
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:24275569; propagated from
                     UniProtKB/Swiss-Prot (Q5U651.1)"
     Region          551..913
                     /region_name="Myo5p-like_CBD_Rasip1"
                     /note="cargo binding domain of myosin 5-like of
                     Ras-interacting protein 1; cd15472"
                     /db_xref="CDD:271256"
     CDS             1..963
                     /gene="RASIP1"
                     /gene_synonym="RAIN"
                     /coded_by="NM_017805.3:95..2986"
                     /db_xref="CCDS:CCDS12731.1"
                     /db_xref="GeneID:54922"
                     /db_xref="HGNC:HGNC:24716"
                     /db_xref="MIM:609623"
ORIGIN      
        1 mlsgerkegg sprfgklhlp vglwinsprk qlaklgrrwp saasvkssss dtgsrssepl
       61 ppppphvelr rvgavkaagg asgsrakris qlfrgsgtgt tgssgaggpg tpggaqrwas
      121 ekklpelaag vapepplatr atappgvlki fgaglasgan yksvlatars tarelvaeal
      181 eryglagspg ggpgesscvd afalcdalgr paaagvgsge wraehlrvlg dserpllvqe
      241 lwrarpgwar rfelrgreea rrleqeafga adsegtgaps wrpqknrsra asggaalasp
      301 gpgtgsgapa gsggkersen lslrrsvsel slqgrrrrqq errqqalsma pgaadaqigt
      361 adpgdfdqlt qcliqapsnr pyflllqgyq daqdfvvyvm treqhvfgrg gnssgrggsp
      421 apyvdtflna pdilprhctv ragpehpamv rpsrgapvth ngclllreae lhpgdllglg
      481 ehflfmykdp rtggsgparp pwlparpgat ppgpgwafsc rlcgrglqer gealaayldg
      541 repvlrfrpr eeeallgeiv raaaagsgdl pplgpatlla lcvqhsarel elghlprllg
      601 rlarlikeav wekikeigdr qpenhpegvp evpltpeavs velrplmlwm anttellsfv
      661 qekvlemeke adqedpqlcn dlelcdeama lldevimctf qqsvyyltkt lystlpalld
      721 snpftagael pgpgaelgam ppglrptlgv fqaaleltsq celhpdlvsq tfgylfffsn
      781 asllnslmer gqgrpfyqws ravqirtnld lvldwlqgag lgdiateffr klsmavnllc
      841 vprtsllkas wsslrtdhpt ltpaqlhhll shyqlgpgrg ppaawdpppa ereavdtgdi
      901 fesfsshppl ilplgssrlr ltgpvtddal hrelrrlrrl lwdleqqelp anyrhgppva
      961 tsp
//
