LOCUS       NM_001139488            4386 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens RAS guanyl releasing protein 3 (RASGRP3), transcript
            variant 1, mRNA.
ACCESSION   NM_001139488
VERSION     NM_001139488.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Zhang,Z., Ma,M., Hu,R., Xu,B., Zong,L., Wei,H. and Meng,Y.
  TITLE     RasGRP3, a Ras guanyl releasing protein 3 that contributes to
            malignant proliferation and aggressiveness in human esophageal
            squamous cell carcinoma
  JOURNAL   Clin Exp Pharmacol Physiol 45 (7), 720-728 (2018)
   PUBMED   29461644
  REMARK    GeneRIF: RasGRP3, a Ras guanyl releasing protein 3 that contributes
            to malignant proliferation and aggressiveness in human esophageal
            squamous cell carcinoma
REFERENCE   3  (bases 1 to 4386)
  AUTHORS   Czikora,A., Kedei,N., Kalish,H. and Blumberg,P.M.
  TITLE     Importance of the REM (Ras exchange) domain for membrane
            interactions by RasGRP3
  JOURNAL   Biochim Biophys Acta Biomembr 1859 (12), 2350-2360 (2017)
   PUBMED   28912101
  REMARK    GeneRIF: The marked differences between RasGRP3 and RasGRP1 in
            membrane interaction necessarily will contribute to their different
            behavior in cells.
REFERENCE   4  (bases 1 to 4386)
  AUTHORS   Hu,G., Zhou,Y., Zhu,Y., Zhou,L., Ling,R., Wu,D., Mi,L., Wang,X.,
            Dai,D., Mao,C. and Chen,D.
  TITLE     Novel transduction of nutrient stress to Notch pathway by RasGRP3
            promotes malignant aggressiveness in human esophageal squamous cell
            carcinoma
  JOURNAL   Oncol Rep 38 (5), 2975-2984 (2017)
   PUBMED   29048643
  REMARK    GeneRIF: In conclusion, these findings provide a new insight into
            the upregulation of RasGRP3 involved in Notch pathway activation in
            the development of esophageal squamous cell carcinoma , especially
            under nutrient deprivation.
REFERENCE   5  (bases 1 to 4386)
  AUTHORS   Qiu,W., Xia,X., Qiu,Z., Guo,M. and Yang,Z.
  TITLE     RasGRP3 controls cell proliferation and migration in papillary
            thyroid cancer by regulating the Akt-MDM2 pathway
  JOURNAL   Gene 633, 35-41 (2017)
   PUBMED   28864115
  REMARK    GeneRIF: these data show that RasGRP3 exerts its oncogenic effect
            in papillary thyroid cancer through Akt-mediated MDM2 activation.
REFERENCE   6  (bases 1 to 4386)
  AUTHORS   Brodie,C., Steinhart,R., Kazimirsky,G., Rubinfeld,H., Hyman,T.,
            Ayres,J.N., Hur,G.M., Toth,A., Yang,D., Garfield,S.H., Stone,J.C.
            and Blumberg,P.M.
  TITLE     PKCdelta associates with and is involved in the phosphorylation of
            RasGRP3 in response to phorbol esters
  JOURNAL   Mol Pharmacol 66 (1), 76-84 (2004)
   PUBMED   15213298
REFERENCE   7  (bases 1 to 4386)
  AUTHORS   Teixeira,C., Stang,S.L., Zheng,Y., Beswick,N.S. and Stone,J.C.
  TITLE     Integration of DAG signaling systems mediated by PKC-dependent
            phosphorylation of RasGRP3
  JOURNAL   Blood 102 (4), 1414-1420 (2003)
   PUBMED   12730099
REFERENCE   8  (bases 1 to 4386)
  AUTHORS   Lorenzo,P.S., Kung,J.W., Bottorff,D.A., Garfield,S.H., Stone,J.C.
            and Blumberg,P.M.
  TITLE     Phorbol esters modulate the Ras exchange factor RasGRP3
  JOURNAL   Cancer Res 61 (3), 943-949 (2001)
   PUBMED   11221888
REFERENCE   9  (bases 1 to 4386)
  AUTHORS   Rebhun,J.F., Castro,A.F. and Quilliam,L.A.
  TITLE     Identification of guanine nucleotide exchange factors (GEFs) for
            the Rap1 GTPase. Regulation of MR-GEF by M-Ras-GTP interaction
  JOURNAL   J Biol Chem 275 (45), 34901-34908 (2000)
   PUBMED   10934204
REFERENCE   10 (bases 1 to 4386)
  AUTHORS   Adams,M.D., Soares,M.B., Kerlavage,A.R., Fields,C. and Venter,J.C.
  TITLE     Rapid cDNA sequencing (expressed sequence tags) from a
            directionally cloned human infant brain cDNA library
  JOURNAL   Nat Genet 4 (4), 373-380 (1993)
   PUBMED   8401585
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA336406.1, DA439087.1,
            BC027849.1, AA351079.1 and BX647990.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001139488.1.
            
            Summary: The protein encoded by this gene is a guanine nucleotide
            exchange factor that activates the oncogenes HRAS and RAP1A.
            Defects in this gene have been associated with systemic lupus
            erythematosus and several cancers. [provided by RefSeq, Mar 2017].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1). Variants 1, 2, and 4-6 all encode the same isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX647990.1, SRR1803611.264331.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000403687.8/ ENSP00000384192.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-139               DA336406.1         355-493
            140-140             DA439087.1         425-425
            141-2575            BC027849.1         449-2883
            2576-2652           AA351079.1         6-82
            2653-2701           BC027849.1         2957-3005
            2702-2702           AA351079.1         130-130
            2703-3135           BC027849.1         3007-3439
            3136-4386           BX647990.1         3132-4382
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p22.3"
     gene            1..4386
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /note="RAS guanyl releasing protein 3"
                     /db_xref="GeneID:25780"
                     /db_xref="HGNC:HGNC:14545"
                     /db_xref="MIM:609531"
     exon            1..59
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            60..192
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            193..389
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /inference="alignment:Splign:2.1.0"
     CDS             320..2392
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /note="isoform 1 is encoded by transcript variant 1;
                     guanine nucleotide exchange factor for Rap1; ras
                     guanyl-releasing protein 3; RAS guanyl releasing protein 3
                     (calcium and DAG-regulated); CalDAG-GEFIII; calcium- and
                     diacylglycerol-regulated guanine nucleotide exchange
                     factor III; calcium and DAG-regulated guanine nucleotide
                     exchange factor III"
                     /codon_start=1
                     /product="ras guanyl-releasing protein 3 isoform 1"
                     /protein_id="NP_001132960.1"
                     /db_xref="CCDS:CCDS46256.1"
                     /db_xref="GeneID:25780"
                     /db_xref="HGNC:HGNC:14545"
                     /db_xref="MIM:609531"
                     /translation="MGSSGLGKAATLDELLCTCIEMFDDNGELDNSYLPRIVLLMHRW
                     YLSSTELAEKLLCMYRNATGESCNEFRLKICYFMRYWILKFPAEFNLDLGLIRMTEEF
                     REVASQLGYEKHVSLIDISSIPSYDWMRRVTQRKKVSKKGKACLLFDHLEPIELAEHL
                     TFLEHKSFRRISFTDYQSYVIHGCLENNPTLERSIALFNGISKWVQLMVLSKPTPQQR
                     AEVITKFINVAKKLLQLKNFNTLMAVVGGLSHSSISRLKETHSHLSSEVTKNWNEMTE
                     LVSSNGNYCNYRKAFADCDGFKIPILGVHLKDLIAVHVIFPDWTEENKVNIVKMHQLS
                     VTLSELVSLQNASHHLEPNMDLINLLTLSLDLYHTEDDIYKLSLVLEPRNSKSQPTSP
                     TTPNKPVVPLEWALGVMPKPDPTVINKHIRKLVESVFRNYDHDHDGYISQEDFESIAA
                     NFPFLDSFCVLDKDQDGLISKDEMMAYFLRAKSQLHCKMGPGFIHNFQEMTYLKPTFC
                     EHCAGFLWGIIKQGYKCKDCGANCHKQCKDLLVLACRRFARAPSLSSGHGSLPGSPSL
                     PPAQDEVFEFPGVTAGHRDLDSRAITLVTGSSRKISVRLQRATTSQATQTEPVWSEAG
                     WGDSGSHTFPKMKSKFHDKAAKDKGFAKWENEKPRVHAGVDVVDRGTEFELDQDEGEE
                     TRQDGEDG"
     exon            390..492
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            493..555
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            556..687
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            688..835
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            836..1009
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1126
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1127..1402
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1480
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1481..1597
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1598..1713
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1714..1861
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1862..1898
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            1899..2024
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            2025..2383
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /inference="alignment:Splign:2.1.0"
     exon            2384..4386
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4226..4231
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /note="hexamer: AATAAA"
     polyA_site      4262
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /note="major polyA site"
     regulatory      4365..4370
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
                     /note="hexamer: AATAAA"
     polyA_site      4386
                     /gene="RASGRP3"
                     /gene_synonym="GRP3"
ORIGIN      
        1 acttggcagc tggacaggca gagtgctgat gtgaaaaata ccacgacaaa accaccctag
       61 ttctccaaaa tacttatcat tcaggttgaa taaaccagtt ctacagactg cttcttccat
      121 gcatttccca atgatgctag ctgcccttaa aattctttca gatttggaac tgtatctgta
      181 tggaaacaac agagttttct tgaagtacaa ctaaggacag gtcaccacta ggcactaaca
      241 tcgctgactt gcatgattat ggagatggtc tatctgatgc tgaaaatgtc tctagttttt
      301 tgacaacggc taaataacca tgggatcaag tggccttggg aaagcagcaa cattagatga
      361 actgctgtgc acttgcattg agatgtttga tgacaatgga gagctggata atagttattt
      421 gccaagaata gttctactga tgcaccgatg gtatttatct tccactgaat tggcagaaaa
      481 acttctctgc atgtatcgaa atgccactgg agaaagctgc aatgaatttc gattaaagat
      541 ctgctacttc atgaggtact ggattctgaa gtttcctgca gagtttaatt tggatcttgg
      601 tttgattcgt atgactgagg aatttcggga agtagctagt caactaggat atgaaaaaca
      661 cgtcagcctc atcgacatat ccagcattcc ttcctatgac tggatgagaa gagtcacaca
      721 gaggaaaaaa gtatccaaga agggaaaagc ctgtctgctg tttgaccatc tggagcccat
      781 tgaattggct gagcacctca cttttctgga gcataaatct tttagaagga tctcattcac
      841 tgattaccaa agctatgtca tccatggctg cctggagaat aatccaacct tggaaagatc
      901 gattgcttta tttaatggaa tctctaagtg ggtccagttg atggttctta gcaaaccaac
      961 cccccagcaa agggcagaag tcatcacaaa gtttatcaat gttgcaaaga agctccttca
     1021 gctcaaaaat tttaacaccc tgatggcagt ggtgggaggc ctcagtcata gttccatttc
     1081 acgcctcaaa gagacccatt ctcatctttc ttcagaagtt acaaagaact ggaatgaaat
     1141 gacagagttg gtctcctcca acggcaatta ctgcaattac cgcaaggcct ttgccgactg
     1201 cgatggcttc aaaatcccca tccttggagt acacttgaaa gacttgatag ctgtccatgt
     1261 cattttccca gactggacag aggagaacaa agtgaacatt gtgaaaatgc accagctctc
     1321 cgttaccctg agtgaactag tctccctgca gaatgcctct caccacttag aacccaacat
     1381 ggatttgatc aacctgctca cgctttccct ggacctctat cacactgaag atgatattta
     1441 caaactgtca ctggtgctgg agcctagaaa ttctaaatcg cagcctacct cccctacgac
     1501 gcccaacaag cctgtggtac ccctggagtg ggcattaggg gtgatgccaa agccagaccc
     1561 cacggtcatc aacaagcaca taaggaaatt agtggagtct gtatttagaa actatgatca
     1621 cgaccatgat gggtacattt cccaagagga ctttgaaagt atagctgcca attttccctt
     1681 cttggattcc ttctgtgttc tggacaaaga tcaggatggc ctaattagta aagatgaaat
     1741 gatggcttac ttcctgagag ctaaatccca actacactgt aaaatgggac caggatttat
     1801 ccataatttt caggagatga cctatctcaa gccaaccttc tgcgaacact gtgcgggatt
     1861 tctctggggc ataatcaagc aaggatacaa atgcaaagac tgtggagcca attgtcacaa
     1921 acagtgcaaa gacctcctgg ttctggcctg caggagattt gcccgggcgc cctccttgag
     1981 cagtggtcat gggtcactgc ctggaagccc ctcgctgccc ccagcgcagg atgaggtgtt
     2041 tgagttccct ggagtcactg ctggacacag ggatttagac agcagagcca tcacactggt
     2101 tacaggctct tctcgcaaga tctctgtgag gctacagagg gccaccacca gccaggccac
     2161 ccagactgaa cctgtctggt cagaggctgg ctggggggac tcggggtccc acaccttccc
     2221 taaaatgaaa tccaagttcc atgacaaagc agcaaaggac aaaggctttg ccaaatggga
     2281 aaatgagaag cccagggtgc atgctggtgt ggatgttgta gaccggggca cggagtttga
     2341 acttgaccag gatgaaggag aagagaccag acaggatggt gaggatggct gacttcaggc
     2401 tgcggaaact gaaggcaata atgttggctt ttggaagggg caagacgaga aactctgaag
     2461 aaagctctga ctctcaggaa gttatctgga aagatacctg gatgtttact gccttgggac
     2521 actgtgggat ctccatgttt ggactatggg acagagaatt gaccctaact aactaactat
     2581 gaactattta tttcctcctc ccctacccct agttaagtgc cacaaagact gtgttatgta
     2641 gtcagtactt ttttctcatg tatctttctc tagaccattt atatacgggt gaaatgaaag
     2701 cttttttgca tgtacttgat actcagtctg taaactcaga cttcgctttt tttgtgagac
     2761 tatcctttca atatttttat aaacttttgt gtgtgctgtg gcagggaaca gttaacaagg
     2821 agtttattta gaggggtttt tttcattttt tgtttttctt gttttgtttt gttttgtttt
     2881 gttcttcttg gtgtgctttt tggatgggac cagaacttaa cattttcctg aggactagga
     2941 agctcatcag acactggaat gcagtgattc ttctaatgat ggtcaactgc aaggagaaac
     3001 tgtttacatc cttcccacta cacagttgct atgatatgta acaagtcaca catgtatata
     3061 tcacacaagt cttaccaatt ctgcgtcact aggaagctat agcatggtcg tgaaagctct
     3121 cctaatactt acttaaatag ccatggaaga aaattatgct tgtcccttct gactgggtta
     3181 agccatcttt cttaagattc ctgaagtaga aatagcaatt aagaaattaa ttcaagtttt
     3241 gacggttaac atttagcaga aaaacaaacc attgaataag ctactaggtg aaatgcaaaa
     3301 caaccatcaa tttttttaaa ctcaataatt ttgtgaaaaa aaaaaccacc accaaacttt
     3361 tggttgtaaa agagaaccct tgttcccttc ctaagaaact gccttccaca attaaagaat
     3421 actcatagaa agaaaataat cataaatcat atttgattaa catttcattt aagcttttaa
     3481 aatatcaatt ttttaaatag ctcccattta cccaaaataa ctagttatgg gtctatttct
     3541 tccatgatta gaggagagag aacttgattc aagatactga aaaatagagc tgggactgag
     3601 cctgtgaatg acaggaatga tctattacca ggtgactagt cggaaactac acagacgtgc
     3661 actggtctgc atttgggggc taaagtgtat atattccatt taaaatggaa tttgtttgta
     3721 tttggggcag tcttcagtaa agcctttctc ctttcttttc tccattgagg acagttctgc
     3781 tcagcaaaat tgttgagtac ctgttctggg caggtcccac gctatatgca caaagttaag
     3841 aaaaacttgg tcttagccct tgggagctga cagcccatgg gcattaaggc aaagtagttc
     3901 cagtgattta aaatacggtt ccaaatacgc taaaaccaac ttgtgccaac cagatttaca
     3961 gattggaaat actgcagatg atgtgaagtt atcagttgga ggagctgtga ttaagctgga
     4021 taataagaga acgtgccatc tgtaaagcac tcagaaggca gccatcccta gatgttggtt
     4081 tcatgtatat tacactatct actactatcc ataaatgcaa taatatgcat gttaacaaca
     4141 ttaaaaacag caaacagcaa tctaagtaca gaaaagcttt ttgtgtgttt aaaaaaattg
     4201 aagaaaattc aggaagaaac gtgttaataa acattgtact gttcttttgc ttctcaaagg
     4261 aattattcac ttgccacttt ggttattttt gagttttcgt acataggaag ttttatattg
     4321 ccagccttcc tgtgataaag atattaaatg taacttgagt aaaaaataaa aaagaataac
     4381 tatgta
//
