LOCUS       NM_001098671            2268 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens RAS guanyl releasing protein 2 (RASGRP2), transcript
            variant 4, mRNA.
ACCESSION   NM_001098671
VERSION     NM_001098671.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Chen,G., Pei,Y., Ye,Q., Xie,Z., Gyawali,L. and Liang,X.
  TITLE     NEDD4L-mediated RASGRP2 suppresses high-glucose and oxLDL-induced
            vascular endothelial cell dysfunctions by activating Rap1 and R-Ras
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1871 (8), 119844 (2024)
   PUBMED   39260747
  REMARK    GeneRIF: NEDD4L-mediated RASGRP2 suppresses high-glucose and
            oxLDL-induced vascular endothelial cell dysfunctions by activating
            Rap1 and R-Ras.
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Liu,Y., Ouyang,Y., Feng,Z., Jiang,Z., Ma,J., Zhou,X., Cai,C.,
            Han,Y., Zeng,S., Liu,S. and Shen,H.
  TITLE     RASGRP2 is a potential immune-related biomarker and regulates
            mitochondrial-dependent apoptosis in lung adenocarcinoma
  JOURNAL   Front Immunol 14, 1100231 (2023)
   PUBMED   36817422
  REMARK    GeneRIF: RASGRP2 is a potential immune-related biomarker and
            regulates mitochondrial-dependent apoptosis in lung adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2268)
  AUTHORS   Takino,J.I.
  TITLE     [Study on the Role of RASGRP2 in Vascular Endothelial Cells]
  JOURNAL   Yakugaku Zasshi 143 (11), 917-922 (2023)
   PUBMED   37914339
  REMARK    GeneRIF: [Study on the Role of RASGRP2 in Vascular Endothelial
            Cells].
REFERENCE   4  (bases 1 to 2268)
  AUTHORS   Kawankar,N., Shetty,S. and Kulkarni,B.
  TITLE     Novel RASGRP2 variants in platelet function defects: Indian study
  JOURNAL   Br J Haematol 197 (3), 377-380 (2022)
   PUBMED   35122233
  REMARK    GeneRIF: Novel RASGRP2 variants in platelet function defects:
            Indian study.
REFERENCE   5  (bases 1 to 2268)
  AUTHORS   Morais,S., Pereira,M., Lau,C., Goncalves,A., Monteiro,C.,
            Goncalves,M., Oliveira,J., Moreira,L., Cruz,E., Santos,R. and
            Lima,M.
  TITLE     CalDAG-GEFI Deficiency in a Family with Symptomatic Heterozygous
            and Homozygous Carriers of a Likely Pathogenic Variant in RASGRP2
  JOURNAL   Int J Mol Sci 22 (22), 12423 (2021)
   PUBMED   34830306
  REMARK    GeneRIF: CalDAG-GEFI Deficiency in a Family with Symptomatic
            Heterozygous and Homozygous Carriers of a Likely Pathogenic Variant
            in RASGRP2.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2268)
  AUTHORS   Toki,S., Kawasaki,H., Tashiro,N., Housman,D.E. and Graybiel,A.M.
  TITLE     Guanine nucleotide exchange factors CalDAG-GEFI and CalDAG-GEFII
            are colocalized in striatal projection neurons
  JOURNAL   J Comp Neurol 437 (4), 398-407 (2001)
   PUBMED   11503142
REFERENCE   7  (bases 1 to 2268)
  AUTHORS   Guo,F.F., Kumahara,E. and Saffen,D.
  TITLE     A CalDAG-GEFI/Rap1/B-Raf cassette couples M(1) muscarinic
            acetylcholine receptors to the activation of ERK1/2
  JOURNAL   J Biol Chem 276 (27), 25568-25581 (2001)
   PUBMED   11292831
REFERENCE   8  (bases 1 to 2268)
  AUTHORS   Clyde-Smith,J., Silins,G., Gartside,M., Grimmond,S., Etheridge,M.,
            Apolloni,A., Hayward,N. and Hancock,J.F.
  TITLE     Characterization of RasGRP2, a plasma membrane-targeted, dual
            specificity Ras/Rap exchange factor
  JOURNAL   J Biol Chem 275 (41), 32260-32267 (2000)
   PUBMED   10918068
REFERENCE   9  (bases 1 to 2268)
  AUTHORS   Kawasaki,H., Springett,G.M., Toki,S., Canales,J.J., Harlan,P.,
            Blumenstiel,J.P., Chen,E.J., Bany,I.A., Mochizuki,N., Ashbacher,A.,
            Matsuda,M., Housman,D.E. and Graybiel,A.M.
  TITLE     A Rap guanine nucleotide exchange factor enriched highly in the
            basal ganglia
  JOURNAL   Proc Natl Acad Sci U S A 95 (22), 13278-13283 (1998)
   PUBMED   9789079
  REMARK    Erratum:[Proc Natl Acad Sci U S A 1999 Jan 5;96(1):318]
REFERENCE   10 (bases 1 to 2268)
  AUTHORS   Kedra,D., Seroussi,E., Fransson,I., Trifunovic,J., Clark,M.,
            Lagercrantz,J., Blennow,E., Mehlin,H. and Dumanski,J.
  TITLE     The germinal center kinase gene and a novel CDC25-like gene are
            located in the vicinity of the PYGM gene on 11q13
  JOURNAL   Hum Genet 100 (5-6), 611-619 (1997)
   PUBMED   9341881
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA286186.1 and AF043723.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001098671.1.
            
            Summary: The protein encoded by this gene is a brain-enriched
            nucleotide exchanged factor that contains an N-terminal GEF domain,
            2 tandem repeats of EF-hand calcium-binding motifs, and a
            C-terminal diacylglycerol/phorbol ester-binding domain. This
            protein can activate small GTPases, including RAS and RAP1/RAS3.
            The nucleotide exchange activity of this protein can be stimulated
            by calcium and diacylglycerol. Four alternatively spliced
            transcript variants encoding two different isoforms have been found
            for this gene. [provided by RefSeq, Jan 2016].
            
            Transcript Variant: This variant (4) differs in the 5' UTR compared
            to variant 2. Variants 2, 3, and 4 all encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF081194.1, U78170.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000394432.8/ ENSP00000377953.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-522               DA286186.1         37-558
            523-2268            AF043723.1         565-2310
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.1"
     gene            1..2268
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /note="RAS guanyl releasing protein 2"
                     /db_xref="GeneID:10235"
                     /db_xref="HGNC:HGNC:9879"
                     /db_xref="MIM:605577"
     exon            1..140
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /inference="alignment:Splign:2.1.0"
     exon            141..284
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /inference="alignment:Splign:2.1.0"
     CDS             212..2041
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /note="isoform a is encoded by transcript variant 4;
                     calcium and diacylglycerol-regulated guanine nucleotide
                     exchange factor I; guanine exchange factor MCG7; calcium
                     and DAG-regulated guanine nucleotide exchange factor I;
                     RAS guanyl nucleotide-releasing protein 2; RAS guanyl
                     releasing protein 2 (calcium and DAG-regulated);
                     cdc25-like protein; F25B3.3 kinase-like protein"
                     /codon_start=1
                     /product="RAS guanyl-releasing protein 2 isoform a"
                     /protein_id="NP_001092141.1"
                     /db_xref="CCDS:CCDS31598.1"
                     /db_xref="GeneID:10235"
                     /db_xref="HGNC:HGNC:9879"
                     /db_xref="MIM:605577"
                     /translation="MAGTLDLDKGCTVEELLRGCIEAFDDSGKVRDPQLVRMFLMMHP
                     WYIPSSQLAAKLLHIYQQSRKDNSNSLQVKTCHLVRYWISAFPAEFDLNPELAEQIKE
                     LKALLDQEGNRRHSSLIDIDSVPTYKWKRQVTQRNPVGQKKRKMSLLFDHLEPMELAE
                     HLTYLEYRSFCKILFQDYHSFVTHGCTVDNPVLERFISLFNSVSQWVQLMILSKPTAP
                     QRALVITHFVHVAEKLLQLQNFNTLMAVVGGLSHSSISRLKETHSHVSPETIKLWEGL
                     TELVTATGNYGNYRRRLAACVGFRFPILGVHLKDLVALQLALPDWLDPARTRLNGAKM
                     KQLFSILEELAMVTSLRPPVQANPDLLSLLTVSLDQYQTEDELYQLSLQREPRSKSSP
                     TSPTSCTPPPRPPVLEEWTSAAKPKLDQALVVEHIEKMVESVFRNFDVDGDGHISQEE
                     FQIIRGNFPYLSAFGDLDQNQDGCISREEMVSYFLRSSSVLGGRMGFVHNFQESNSLR
                     PVACRHCKALILGIYKQGLKCRACGVNCHKQCKDRLSVECRRRAQSVSLEGSAPSPSP
                     MHSHHHRAFSFSLPRPGRRGSRPPEIREEEVQTVEDGVFDIHL"
     exon            285..387
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /inference="alignment:Splign:2.1.0"
     exon            388..450
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /inference="alignment:Splign:2.1.0"
     exon            451..582
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /inference="alignment:Splign:2.1.0"
     exon            583..733
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /inference="alignment:Splign:2.1.0"
     exon            734..907
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /inference="alignment:Splign:2.1.0"
     exon            908..1024
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /inference="alignment:Splign:2.1.0"
     exon            1025..1306
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /inference="alignment:Splign:2.1.0"
     exon            1307..1384
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /inference="alignment:Splign:2.1.0"
     exon            1385..1507
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1623
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1765
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /inference="alignment:Splign:2.1.0"
     exon            1766..1802
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /inference="alignment:Splign:2.1.0"
     exon            1803..1982
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2047
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /inference="alignment:Splign:2.1.0"
     exon            2048..2268
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2240..2245
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /note="hexamer: AATAAA"
     polyA_site      2268
                     /gene="RASGRP2"
                     /gene_synonym="CALDAG-GEFI; CDC25L"
                     /note="major polyA site"
ORIGIN      
        1 actgccttgc gtgtgtgcac acgtgtgagg atgtcagcgc ctgtgtggcc gcgggactca
       61 aggctggcct ggctcaagtg aacagcacgt ccaggaggcg acctcgtccg cgggtttgca
      121 ttctggggtg gacgagctgg gggttcggtc cgagcccggt gggaggctcc cggagcgcag
      181 cctgggccca gcccaccccg cgccggcggc catggcaggc accctggacc tggacaaggg
      241 ctgcacggtg gaggagctgc tccgcgggtg catcgaagcc ttcgatgact ccgggaaggt
      301 gcgggacccg cagctggtgc gcatgttcct catgatgcac ccctggtaca tcccctcctc
      361 tcagctggcg gccaagctgc tccacatcta ccaacaatcc cggaaggaca actccaattc
      421 cctgcaggtg aaaacgtgcc acctggtcag gtactggatc tccgccttcc cagcggagtt
      481 tgacttgaac ccggagttgg ctgagcagat caaggagctg aaggctctgc tagaccaaga
      541 agggaaccga cggcacagca gcctaatcga catagacagc gtccctacct acaagtggaa
      601 gcggcaggtg actcagcgga accctgtggg acagaaaaag cgcaagatgt ccctgttgtt
      661 tgaccacctg gagcccatgg agctggcgga gcatctcacc tacttggagt atcgctcctt
      721 ctgcaagatc ctgtttcagg actatcacag tttcgtgact catggctgca ctgtggacaa
      781 ccccgtcctg gagcggttca tctccctctt caacagcgtc tcacagtggg tgcagctcat
      841 gatcctcagc aaacccacag ccccgcagcg ggccctggtc atcacacact ttgtccacgt
      901 ggcggagaag ctgctacagc tgcagaactt caacacgctg atggcagtgg tcgggggcct
      961 gagccacagc tccatctccc gcctcaagga gacccacagc cacgttagcc ctgagaccat
     1021 caagctctgg gagggtctca cggaactagt gacggcgaca ggcaactatg gcaactaccg
     1081 gcgtcggctg gcagcctgtg tgggcttccg cttcccgatc ctgggtgtgc acctcaagga
     1141 cctggtggcc ctgcagctgg cactgcctga ctggctggac ccagcccgga cccggctcaa
     1201 cggggccaag atgaagcagc tctttagcat cctggaggag ctggccatgg tgaccagcct
     1261 gcggccacca gtacaggcca accccgacct gctgagcctg ctcacggtgt ctctggatca
     1321 gtatcagacg gaggatgagc tgtaccagct gtccctgcag cgggagccgc gctccaagtc
     1381 ctcgccaacc agccccacga gttgcacccc accaccccgg cccccggtac tggaggagtg
     1441 gacctcggct gccaaaccca agctggatca ggccctcgtg gtggagcaca tcgagaagat
     1501 ggtggagtct gtgttccgga actttgacgt cgatggggat ggccacatct cacaggaaga
     1561 attccagatc atccgtggga acttccctta cctcagcgcc tttggggacc tcgaccagaa
     1621 ccaggatggc tgcatcagca gggaggagat ggtttcctat ttcctgcgct ccagctctgt
     1681 gttggggggg cgcatgggct tcgtacacaa cttccaggag agcaactcct tgcgccccgt
     1741 cgcctgccgc cactgcaaag ccctgatcct gggcatctac aagcagggcc tcaaatgccg
     1801 agcctgtgga gtgaactgcc acaagcagtg caaggatcgc ctgtcagttg agtgtcggcg
     1861 cagggcccag agtgtgagcc tggaggggtc tgcaccctca ccctcaccca tgcacagcca
     1921 ccatcaccgc gccttcagct tctctctgcc ccgccctggc aggcgaggct ccaggcctcc
     1981 agagatccgt gaggaggagg tacagacggt ggaggatggg gtgtttgaca tccacttgta
     2041 atagatgctg tggttggatc aaggactcat tcctgccttg gagaaaatac ttcaaccaga
     2101 gcagggagcc tgggggtgtc ggggcaggag gctggggatg ggggtgggat atgagggtgg
     2161 catgcagctg agggcagggc cagggctggt gtccctaagg ttgtacagac tcttgtgaat
     2221 atttgtattt tccagatgga ataaaaaggc ccgtgtaatt aaccttca
//
