LOCUS       NM_006909               8482 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens Ras protein specific guanine nucleotide releasing
            factor 2 (RASGRF2), mRNA.
ACCESSION   NM_006909 XM_027943
VERSION     NM_006909.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Lin,Q., Qiu,M., Wei,X., Xiang,Z., Zhou,Z., Ji,I., Liang,X.,
            Zhou,X., Wen,Q., Liu,Y. and Yu,H.
  TITLE     Genetic variants of SOS2, MAP2K1 and RASGRF2 in the RAS pathway
            genes predict survival of HBV-related hepatocellular carcinoma
            patients
  JOURNAL   Arch Toxicol 97 (6), 1599-1611 (2023)
   PUBMED   37029817
  REMARK    GeneRIF: Genetic variants of SOS2, MAP2K1 and RASGRF2 in the RAS
            pathway genes predict survival of HBV-related hepatocellular
            carcinoma patients.
REFERENCE   2  (bases 1 to 8482)
  AUTHORS   Korolkova,O.Y., Widatalla,S.E., Whalen,D.S., Nangami,G.N.,
            Abimbola,A., Williams,S.D., Beasley,H.K., Reisenbichler,E.,
            Washington,M.K., Ochieng,J., Mayer,I.A., Lehmann,B.D. and
            Sakwe,A.M.
  TITLE     Reciprocal expression of Annexin A6 and RasGRF2 discriminates
            rapidly growing from invasive triple negative breast cancer subsets
  JOURNAL   PLoS One 15 (4), e0231711 (2020)
   PUBMED   32298357
  REMARK    GeneRIF: AnxA6 may be a reliable biomarker for distant relapse-free
            survival and response of triple negative breast cancer (TNBC)
            patients to chemotherapy, and that the reciprocal expression of
            AnxA6 and GRF2 can reliably delineate TNBCs into rapidly growing
            and invasive subsets which may be more relevant for subset-specific
            therapeutic interventions.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8482)
  AUTHORS   Lu,P., Chen,J., Yan,L., Yang,L., Zhang,L., Dai,J., Hao,Z., Bai,T.,
            Xi,Y., Li,Y., Kang,Z., Xv,J., Sun,G. and Yang,T.
  TITLE     RasGRF2 promotes migration and invasion of colorectal cancer cells
            by modulating expression of MMP9 through Src/Akt/NF-kappaB pathway
  JOURNAL   Cancer Biol Ther 20 (4), 435-443 (2019)
   PUBMED   30359168
  REMARK    GeneRIF: High RASGRF2 expression is associated with migration and
            invasion of colorectal cancer.
REFERENCE   4  (bases 1 to 8482)
  AUTHORS   Karamitros,T., Hurst,T., Marchi,E., Karamichali,E.,
            Georgopoulou,U., Mentis,A., Riepsaame,J., Lin,A., Paraskevis,D.,
            Hatzakis,A., McLauchlan,J., Katzourakis,A. and Magiorkinis,G.
  TITLE     Human Endogenous Retrovirus-K HML-2 integration within RASGRF2 is
            associated with intravenous drug abuse and modulates transcription
            in a cell-line model
  JOURNAL   Proc Natl Acad Sci U S A 115 (41), 10434-10439 (2018)
   PUBMED   30249655
  REMARK    GeneRIF: RASGRF2-int is significantly associated with drug
            addiction.
REFERENCE   5  (bases 1 to 8482)
  AUTHORS   Novo-Veleiro,I., Cieza-Borrella,C., Pastor,I., Chamorro,A.J.,
            Laso,F.J., Gonzalez-Sarmiento,R. and Marcos,M.
  TITLE     A Single Nucleotide Polymorphism in the RASGRF2 Gene Is Associated
            with Alcoholic Liver Cirrhosis in Men
  JOURNAL   PLoS One 11 (12), e0168685 (2016)
   PUBMED   27992614
  REMARK    GeneRIF: this genetic association study represents the first to
            show an association of the RASGRF2 G>A (rs26907) polymorphism with
            Alcoholic Liver Cirrhosis in men, particularly in the subgroup of
            patients with alcohol dependence. The findings suggest the
            potential relevance of the RAS gene family in alcoholism and
            Alcoholic Liver Cirrhosis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 8482)
  AUTHORS   Harrington,A.W., Kim,J.Y. and Yoon,S.O.
  TITLE     Activation of Rac GTPase by p75 is necessary for c-jun N-terminal
            kinase-mediated apoptosis
  JOURNAL   J Neurosci 22 (1), 156-166 (2002)
   PUBMED   11756498
REFERENCE   7  (bases 1 to 8482)
  AUTHORS   Anborgh,P.H., Qian,X., Papageorge,A.G., Vass,W.C., DeClue,J.E. and
            Lowy,D.R.
  TITLE     Ras-specific exchange factor GRF: oligomerization through its Dbl
            homology domain and calcium-dependent activation of Raf
  JOURNAL   Mol Cell Biol 19 (7), 4611-4622 (1999)
   PUBMED   10373510
REFERENCE   8  (bases 1 to 8482)
  AUTHORS   Fan,W.T., Koch,C.A., de Hoog,C.L., Fam,N.P. and Moran,M.F.
  TITLE     The exchange factor Ras-GRF2 activates Ras-dependent and
            Rac-dependent mitogen-activated protein kinase pathways
  JOURNAL   Curr Biol 8 (16), 935-938 (1998)
   PUBMED   9707409
REFERENCE   9  (bases 1 to 8482)
  AUTHORS   Fam,N.P., Fan,W.T., Wang,Z., Zhang,L.J., Chen,H. and Moran,M.F.
  TITLE     Cloning and characterization of Ras-GRF2, a novel guanine
            nucleotide exchange factor for Ras
  JOURNAL   Mol Cell Biol 17 (3), 1396-1406 (1997)
   PUBMED   9032266
REFERENCE   10 (bases 1 to 8482)
  AUTHORS   Fam,N.P., Zhang,L.J., Rommens,J.M., Beatty,B.G. and Moran,M.F.
  TITLE     Mapping of the Ras-GRF2 gene (GRF2) to mouse chromosome 13C3-D1 and
            human chromosome 5q13, near the Ras-GAP gene
  JOURNAL   Genomics 39 (1), 118-120 (1997)
   PUBMED   9027497
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC026427.4, BC136296.1,
            DA219722.1, DA230428.1, BX502901.1, BU430075.1, CR749239.1 and
            BC041953.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_006909.2.
            
            Summary: RAS GTPases cycle between an inactive GDP-bound state and
            an active GTP-bound state. This gene encodes a calcium-regulated
            nucleotide exchange factor activating both RAS and RAS-related
            protein, RAC1, through the exchange of bound GDP for GTP, thereby,
            coordinating the signaling of distinct mitogen-activated protein
            kinase pathways. [provided by RefSeq, Oct 2011].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.174237.1,
                                           SRR1803614.86111.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000265080.9/ ENSP00000265080.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-326               AC026427.4         172257-172582       c
            327-4132            BC136296.1         1-3806
            4133-4543           DA219722.1         155-565
            4544-5044           DA230428.1         157-657
            5045-5341           BX502901.1         315-611
            5342-5696           BU430075.1         105-459
            5697-7543           CR749239.1         3392-5238
            7544-8482           BC041953.1         1495-2433
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q14.1"
     gene            1..8482
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /note="Ras protein specific guanine nucleotide releasing
                     factor 2"
                     /db_xref="GeneID:5924"
                     /db_xref="HGNC:HGNC:9876"
                     /db_xref="MIM:606614"
     exon            1..664
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     CDS             377..4090
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /note="ras guanine nucleotide exchange factor 2"
                     /codon_start=1
                     /product="ras-specific guanine nucleotide-releasing factor
                     2"
                     /protein_id="NP_008840.1"
                     /db_xref="CCDS:CCDS4052.1"
                     /db_xref="GeneID:5924"
                     /db_xref="HGNC:HGNC:9876"
                     /db_xref="MIM:606614"
                     /translation="MQKSVRYNEGHALYLAFLARKEGTKRGFLSKKTAEASRWHEKWF
                     ALYQNVLFYFEGEQSCRPAGMYLLEGCSCERTPAPPRAGAGQGGVRDALDKQYYFTVL
                     FGHEGQKPLELRCEEEQDGKEWMEAIHQASYADILIEREVLMQKYIHLVQIVETEKIA
                     ANQLRHQLEDQDTEIERLKSEIIALNKTKERMRPYQSNQEDEDPDIKKIKKVQSFMRG
                     WLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAESEYVHQLYILVNGFLRPLRMA
                     ASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLILADLFDILLPMLN
                     IYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYI
                     ITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGC
                     DILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRS
                     SGGKLHLLKTGGVLSLIDCTLIEEPDASDDDSKGSGQVFGHLDFKIVVEPPDAAAFTV
                     VLLAPSRQEKAAWMSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTD
                     ICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLGKL
                     SDIYKRPFTSIPVRSLELFFATSQNNRGEHLVDGKSPRLCRKFSSPPPLAVSRTSSPV
                     RARKLSLTSPLNSKIGALDLTTSSSPTTTTQSPAASPPPHTGQIPLDLSRGLSSPEQS
                     PGTVEENVDNPRVDLCNKLKRSIQKAVLESAPADRAGVESSPAADTTELSPCRSPSTP
                     RHLRYRQPGGQTADNAHCSVSPASAFAIATAAAGHGSPPGFNNTERTCDKEFIIRRTA
                     TNRVLNVLRHWVSKHAQDFELNNELKMNVLNLLEEVLRDPDLLPQERKAAANILRALS
                     QDDQDDIHLKLEDIIQMTDCMKAECFESLSAMELAEQITLLDHVIFRSIPYEEFLGQG
                     WMKLDKNERTPYIMKTSQHFNDMSNLVASQIMNYADVSSRANAIEKWVAVADICRCLH
                     NYNGVLEITSALNRSAIYRLKKTWAKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNC
                     NPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTSYRIDH
                     QPKVAQYLLDKDLIIDEDTLYELSLKIEPRLPA"
     exon            665..771
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            772..919
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1009
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1010..1263
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1264..1343
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1344..1537
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1538..1647
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1648..1766
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1767..1927
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1928..1994
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1995..2131
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2132..2214
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2215..2463
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2464..2846
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2847..2972
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2973..3062
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3063..3169
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3170..3282
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3283..3343
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3344..3447
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3448..3532
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3533..3730
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3731..3810
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3811..3928
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3929..3997
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3998..8482
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8455..8460
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /note="hexamer: ATTAAA"
     polyA_site      8482
                     /gene="RASGRF2"
                     /gene_synonym="GRF2; RAS-GRF2"
                     /note="major polyA site"
ORIGIN      
        1 agtccttccc cggctgccgc cccagcccgc cgcgggggct cggctcccgc aactttgggc
       61 gaagcggcgg ccggctcggg cgccctcgcg gggccgcgct ccacgcgggc gttgggggct
      121 tggggggctc cgggggctcg gccgggaggg tggtggttag ggcggagagc gtgcctcggc
      181 cccagcccgc gcccctgcca ccgcgcgccg cggcctcccg aaagcgggcg gggtgccctg
      241 cgcgcggcgt ggggaaaggg ggcgcccttc gccggccggg acctgagcgg tcgcgccctc
      301 gagggagcca gctgggccat ggccgcgagg caggggtgag accggcggcc acccgtgagc
      361 cctccgcacc cgcaccatgc agaagagcgt gcgctacaac gaggggcacg ccctgtacct
      421 ggcctttctg gcgcgcaagg agggcaccaa gcgcggcttc ctgagtaaga agacggccga
      481 ggcgagccgc tggcacgaga agtggttcgc cctctaccag aatgtgctct tctacttcga
      541 gggcgagcag agctgccgcc cggcgggcat gtacctcctg gagggctgca gctgcgaacg
      601 aacgcccgcg ccacccaggg ccggcgccgg gcagggaggc gtccgagacg cgctggacaa
      661 gcagtattac tttactgttc tttttggcca tgaaggtcag aagccactgg agctgcgctg
      721 tgaggaggag caggatggta aagagtggat ggaggccatt caccaagcca gttatgcaga
      781 cattttgatt gagagggaag tattaatgca gaagtacatt catctagttc agatcgtaga
      841 gacagaaaaa attgcagcta accaactccg acatcaactt gaagatcaag acacagaaat
      901 cgaaaggctt aaatcagaga ttattgctct taataaaacc aaagaacgaa tgcgacctta
      961 ccaaagcaac caagaagacg aagatccaga catcaagaag attaaaaagg ttcagagctt
     1021 catgcgagga tggttgtgca gaaggaaatg gaagaccatc gtgcaggatt acatttgttc
     1081 tcctcatgct gaaagtatga ggaagagaaa ccagattgtg ttcaccatgg tggaggcaga
     1141 gtcagagtac gttcaccagc tctacatcct ggtcaatggc tttctccggc ccctgcgtat
     1201 ggccgccagc tccaagaagc cccccatcag ccacgacgac gtcagcagta tttttcttaa
     1261 cagtgaaaca atcatgtttc ttcatgaaat atttcatcaa ggactaaagg caaggatagc
     1321 aaactggccc actttaattt tagctgatct gtttgatatt ttgctcccca tgctgaacat
     1381 ttatcaagaa tttgtgcgta atcaccagta cagcctgcaa gttctcgcca attgtaagca
     1441 aaacagagat tttgacaaac tcttaaaaca gtatgaagcc aatccagcct gtgaggggag
     1501 gatgctggag acattcttga cctatcccat gtttcagatc cccagatata tcatcacact
     1561 ccatgagctc cttgctcaca caccccatga gcatgtggaa aggaaaagcc tggagtttgc
     1621 caaatcaaag ctagaggaac tatccagagt aatgcacgat gaagtcagcg acactgaaaa
     1681 cataaggaaa aaccttgcca tcgaaagaat gatcgtggag ggctgtgaca tcttgctgga
     1741 caccagccaa acgttcatcc gccaaggttc tcttattcaa gtaccttccg ttgagagggg
     1801 gaaacttagt aaagttcgcc tgggttcgtt gtctttgaaa aaggaaggag agagacaatg
     1861 cttcttattt acaaaacact ttttaatatg tacaagaagt tcaggaggga agcttcatct
     1921 gctcaagaca ggtggggttc tgtctctaat agactgcaca ttgattgagg agccagatgc
     1981 aagcgatgat gactctaaag gttctgggca agtgtttggg cacctggatt ttaaaatagt
     2041 ggtggagcct cctgacgctg ccgccttcac tgttgtcttg ttagcaccct cacgccagga
     2101 gaaagctgcc tggatgagtg acatcagtca gtgtgtggac aatatacgat gtaatggttt
     2161 aatgactata gtgtttgaag agaattccaa agtcactgtg ccacatatga ttaagtctga
     2221 tgcccgtctt cataaagacg acactgacat ttgcttcagt aaaacactca actcctgcaa
     2281 agtgccccag atccgttatg ccagcgtgga gcgcctcttg gaacgactga cagacttgcg
     2341 gtttcttagt attgatttcc tcaacacctt tctgcacacc tatcgtattt tcactactgc
     2401 cgctgtggtg ctggggaaac tctccgacat atacaagagg cctttcacct ccatccctgt
     2461 caggtcattg gaattgtttt ttgctaccag ccagaacaac agaggtgaac atttggtgga
     2521 tggcaaatcc ccacgtctgt gtcgcaaatt ctcttccccg ccaccactgg ctgtgtccag
     2581 aacatcttcc ccagtgaggg ccagaaagct gtctttgact tctcccttga actcaaagat
     2641 aggagcattg gacctgacaa cttccagcag tcccaccacc accacccaga gtcccgctgc
     2701 gtctccacca ccacacactg gtcagatacc actggatctc agcagaggcc tctcttctcc
     2761 agagcaaagc ccgggaacgg tagaagagaa tgtcgataac ccacgcgtgg atctgtgtaa
     2821 caagctaaaa cgaagtattc aaaaagcagt cctagagtct gcaccagcgg accgagcagg
     2881 agtggaaagc tcccctgcag cggacaccac agaactttca ccttgcagat ccccctcaac
     2941 tcctcggcac ctccgctatc gacagcctgg aggacagacg gcggacaatg cccactgctc
     3001 tgtttcaccg gcttctgctt ttgcaatagc cacagctgca gcaggacatg ggagtccacc
     3061 aggctttaac aacaccgaga gaacatgtga taaagagttt attatacgga gaacggctac
     3121 caatcgagtt ctgaacgtcc tccgtcactg ggtctcaaag cacgcacagg atttcgaact
     3181 caacaatgaa ctaaagatga atgtcctaaa tttgctagaa gaagttttgc gagacccaga
     3241 ccttcttccc caagaaagga aagccgccgc gaatatcctc agggcccttt cacaagatga
     3301 ccaagatgac atccacctaa aattagagga tataattcaa atgactgact gcatgaaggc
     3361 cgaatgcttt gagtccttgt cggccatgga gctggcagaa cagatcaccc tcctggacca
     3421 tgtcattttc agaagcattc cctacgagga gtttcttggg caggggtgga tgaagctgga
     3481 taaaaacgaa agaactcctt acattatgaa aaccagccaa cacttcaatg acatgagtaa
     3541 cctggtggcc tcccagataa tgaactatgc tgatgtcagc tcccgtgcca acgccatcga
     3601 gaaatgggtg gcagtggcgg acatctgccg atgcctgcac aactacaacg gcgtgctgga
     3661 gatcacctcg gccttaaaca gaagtgccat ctacaggctg aagaaaacct gggccaaggt
     3721 ctctaagcag acaaaagctc taatggacaa acttcaaaag actgtttcct ctgaaggaag
     3781 atttaaaaat cttagagaaa cccttaaaaa ttgtaaccct cctgcagttc cttatcttgg
     3841 gatgtacttg acagacctgg catttattga agaaggaaca ccaaacttta ctgaggaagg
     3901 ccttgtcaat ttctccaaaa tgagaatgat atcacacatc atcagagaga tacgccagtt
     3961 ccagcagact tcctacagaa tagatcatca gccaaaggtc gcacagtact tgcttgacaa
     4021 agaccttatc atagatgaag atacgctata tgagctgtca ctaaaaattg aacctcgact
     4081 ccctgcttga agatctggcc ttgcccctga gtccacggga tgttcatgga aagcaggaca
     4141 gacagaattg tgtatgcctt gcctatcacg gtacagcacg aagccaggct cctttctcca
     4201 ccaaagaaga tggaaccaga ctggaattct gtctccagag agaaacccag ctgtttgggt
     4261 caaagacaga tgcttcagac ttgggtggga aggtgaaaga tggctattta gaaagctggt
     4321 ggcacgtttt acataaggga atgtcagatg ggagatgcta gttgccattt taacaaagca
     4381 ggtaaatcgg taaattttaa actctgtcca tgttctgtta gaactcaggg acaaggatcc
     4441 atgaaaaaga cctgtgatgt ttctctggcg ctttactggc ctgggcacac ctaccaatct
     4501 tctaggattt gactggttcc attacatttc cttttggtat aagcttcaca gaaaagctga
     4561 cacttcctct acagagatgg accaaaacat aagcaatttc agtctacagc atgtgcatgg
     4621 ttgtcagtgc attctaaata tttctatgtg aggaatggta ccttctgaaa ctgcctttcc
     4681 agtctttagg caatgggata ggaaagaaag aatgaaacac aaatggattt gtatgtaaca
     4741 tttccttaat taaatgcagt aggctgtgcc ccagaggatt ccagacagtg gctggctgag
     4801 gtgggtgggg agcttttcct tgagactgtt ggtcctaaga agccagccct tttggagagg
     4861 cagctgcaaa aaggtgcacg cccatctcac cgacaaaact gtggaacaga aggccaccaa
     4921 gtgctgtggg gaatcatggg tttcagtgct gagtgaaaat ctatacctaa aaatcatctc
     4981 tgcaccttgc tttgtttgtt ttctttcccc actcatagta ctgcaggaat ctattctcat
     5041 ttacacagac ctttttttag gcttactatg aacattggct gtattttttt taaacagttt
     5101 agtgaaattt tcttttcaaa acccacactt ccatatgctg ttcgtagatc tctttcttta
     5161 aaaactgatg ttgagagatc tctgagaata ttataagtgc atgggaaatg ggcccaacca
     5221 ccgaacagct cttacattac aaaaccaaat gcaagggtta gtcctgctac ctgaggctgg
     5281 ggaagtgacc ttccttttcc caagattgtc agttgttgaa gaaatagggc tatctcattg
     5341 tttacctccc tcttctcttc tcagggagac tgctgcttta aaagaaggaa gagaaaaaat
     5401 atagttctat ttccctgaac ctgttgcacc tgacattttc tcttagcagc atgaaactta
     5461 ttgatgctga caatgaaaaa tggatctgtc tggctgcttt ccctctttcc ttgcacttta
     5521 attatgttgc tagagctaac agactaataa attccacctg ctggctctta agactcagtg
     5581 aaagagctag cattggtaat gcaccataga ggtagagaat gtacactttc tgcacggtaa
     5641 gtgccatctc tgtatgtaac tatatagtga aatatcaact aagtaaaaga aaatataata
     5701 tttgaagacc attcccaaaa tattttcaat agttcatatt agccaacagt gtagcactca
     5761 acccaaggag ggttccttat ggatgctttc tttttctttt ttaaagttgc ttgtttgttc
     5821 tctttagttt caaataagag gttgacgcat cttgatgcat gatgagaagc atgggctgtt
     5881 tggatcctaa caacgcataa cttgtgattt atttctcagt gctccagaaa ctgagggttt
     5941 gaaataatat gtatcagttg caccaaacac ctcaaggtct tgcagaagaa aagtaaaggt
     6001 tagctttcat ggctcaaaag catagtcctg aagggtgaac taaaaccggg acaaatctgt
     6061 gagaggacca cacacatact agtttcgggc caaacaacac gtggaaaggt gcatgcattc
     6121 tactctgcct tggagttgcc agagtccttc agagggaaag ggatggttct gtgtgcactt
     6181 tttctggaag ttcggactca tttctttgac ccaaatgttc cagagacact gcagccattc
     6241 ttattaacaa aaaataagac aggagtttcc aaatgctcct tcccttttgg atcgcagctt
     6301 ttcttcaact agtgacaaag cttttgcgcc tatttcctgc aggatgttgg aactgccccg
     6361 cactggtcat attaggcact gtcaattgct atgctgactt ttagggggtt tttgtttgtt
     6421 tgaaaaacag ggtctcacca tgttgcccag gctggtctcg aactcctgga ctcaagcaat
     6481 cttcctgcct cagcctctca agcagctggg actgcagggg tgtgccactc actagccttt
     6541 cgcatttttg tttgagaatt acaccacttt ctggagtctg cagccttcct ggagctgcaa
     6601 gagggcaaga gagagagctc cacctctgag ggagtgtctg ttgatgacct gcactattcg
     6661 tgtgccagct gggagaggaa tgcacatttt aaaatccctt caatttggtc aaattaaaaa
     6721 tccccaagag caatttgcag tgttttttct ggtcgttaaa gtacccatcc tcttctgcct
     6781 acacacaaag catgcattcc cagctgcatc tgcctctagt ccattatgga gacccatttc
     6841 taagaggaga tgggaggtca acctctaaca gccaagtagc gaacatgtat actgtaaaat
     6901 taacctagaa aatcagaaga aaaatccaat ttcatgcttt cgaatgaatg cccacatttt
     6961 gtactgtcaa cgaaattatc ttggagcttt taggggatgc cttttcgtta ttaactgaga
     7021 catctagttt tgctacaggg acaaatctct tacctaatcc aatatattat ttgacagatt
     7081 caggcatgaa gtaaaacgtc gtcacttttc cttagtgctt ttctgaagga atttaaagac
     7141 ggaattttaa acggccattg caatattttc aagtggctct cataccaagt cccattactg
     7201 tttgttaaat ttcagtacgt cttaaagtac tacttataaa caaatgaaac tcagagaaac
     7261 tgaatcacct ggaagagaaa aatccattat ggtcccatgt ggagtgaata atgatggatc
     7321 agcacccttt ctctcatgtt attgtataag acgagacttt tgggccagca gcgattgggc
     7381 agcttttaaa ttcttaactg aaaagagtaa tgcaatacag ggattattcc caataaaatt
     7441 aacttttatt taaaagcaag agattttact tagctttttt ttttcaaagt ttgattttat
     7501 ccccttgaaa aaaaatctct tcactttaaa gtataaaggt ttttaaaaat ccaattgcaa
     7561 aatgtattat ttttacaact atcgaaaagg cataaaagag aacatactat ttatggctga
     7621 agggtatagc caggctaatg tgcacagagg gaatcaataa ataaaactct ttttcatttc
     7681 agtaagaaat cagattgtaa gtttaatggc tccattatag ataccaccgt gtaatagaag
     7741 acttaagtca atgaaatcta atcagtgtgt catttctcag cggccattgg tgacttaaaa
     7801 ttaagatgag gcagagccaa aatggaaaac agtcattttg ttgtaggtat aaacacatga
     7861 acgattcaga aaattattca tctcagctgc catgcagcat gacattaaca ttaggattga
     7921 tagcactagt ctgatctgct caaggaaaat aatagttcta ttatacttaa tgatgttggt
     7981 ttttacacag ctcatttcat ttttcactag aaagccagtt atgaaagaga gctggcctag
     8041 gcatcccggc cctgagtcct aggcccagtc tccaactgga aaaccttagg ctggtgttta
     8101 cacatccctg agcctcagtt tcctcatctg caaaacggtg tgaatagtaa tccctgtgct
     8161 gcttatctca cagggctatt gtgaggacca aatggattag actgtaaact gcaaagtgct
     8221 gtccgcacat gaggtcatct gattactgtc ctcagatctc ttttgtagag gatttcaatg
     8281 tatttcttta tcatttgagt gtgtgtgtga tggacgaata tgtgtgtgag tttgagaagc
     8341 atatcgttcg tgtccagtta ctttgcaaat ttgtggacat ttgtgattgg acagaggggt
     8401 ttgtgctgtg gcctaacact tgccaagtga ggtgtaggtt atgcctatat gcaaattaaa
     8461 cttcaccttt cttgaatatt ca
//
