LOCUS       NM_001145648            6294 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens Ras protein specific guanine nucleotide releasing
            factor 1 (RASGRF1), transcript variant 3, mRNA.
ACCESSION   NM_001145648
VERSION     NM_001145648.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Goto,K., Pissaloux,D., Fraitag,S., Amini,M., Vaucher,R., Tirode,F.
            and de la Fouchardiere,A.
  TITLE     RASGRF1-rearranged Cutaneous Melanocytic Neoplasms With Spitzoid
            Cytomorphology: A Clinicopathologic and Genetic Study of 3 Cases
  JOURNAL   Am J Surg Pathol 46 (5), 655-663 (2022)
   PUBMED   34799483
  REMARK    GeneRIF: RASGRF1-rearranged Cutaneous Melanocytic Neoplasms With
            Spitzoid Cytomorphology: A Clinicopathologic and Genetic Study of 3
            Cases.
REFERENCE   2  (bases 1 to 6294)
  AUTHORS   Cifelli,J.L., Berg,K.R. and Yang,J.
  TITLE     Benzothiazole amphiphiles promote RasGRF1-associated dendritic
            spine formation in human stem cell-derived neurons
  JOURNAL   FEBS Open Bio 10 (3), 386-395 (2020)
   PUBMED   31943943
  REMARK    GeneRIF: Benzothiazole amphiphiles promote RasGRF1-associated
            dendritic spine formation in human stem cell-derived neurons.
REFERENCE   3  (bases 1 to 6294)
  AUTHORS   Kunceviciene,E., Sriubiene,M., Liutkeviciene,R., Miceikiene,I.T.
            and Smalinskiene,A.
  TITLE     Heritability of myopia and its relation with GDJ2 and RASGRF1 genes
            in Lithuania
  JOURNAL   BMC Ophthalmol 18 (1), 124 (2018)
   PUBMED   29793445
  REMARK    GeneRIF: Our studies have shown that the heritability of myopia
            makes 66.4% in Lithuania. We detected significant associations
            between the combinations of GJD2 CC and RASGRF1 GT and odds ratio
            of developing myopia.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6294)
  AUTHORS   Chen,H., Xu,Z., Yang,B., Zhou,X. and Kong,H.
  TITLE     RASGRF1 Hypermethylation, a Putative Biomarker of Colorectal Cancer
  JOURNAL   Ann Clin Lab Sci 48 (1), 3-10 (2018)
   PUBMED   29530990
  REMARK    GeneRIF: Low RASGRF1 expression due to hypermethylation is
            associated with Colorectal Cancer.
REFERENCE   5  (bases 1 to 6294)
  AUTHORS   Hepei,L., Mingkun,X., Li,W. and Jin,W.
  TITLE     Assessment of BicC family RNA binding protein 1 and Ras protein
            specific guanine nucleotide releasing factor 1 as candidate genes
            for high myopia: A case-control study
  JOURNAL   Indian J Ophthalmol 65 (10), 926-930 (2017)
   PUBMED   29044055
  REMARK    GeneRIF: genetic variants in BICC1 and RASGRF1 are closely
            associated with high myopia, which appears to be a potential
            candidate for high myopia in Chinese Han population.
REFERENCE   6  (bases 1 to 6294)
  AUTHORS   Brambilla,R., Gnesutta,N., Minichiello,L., White,G., Roylance,A.J.,
            Herron,C.E., Ramsey,M., Wolfer,D.P., Cestari,V., Rossi-Arnaud,C.,
            Grant,S.G., Chapman,P.F., Lipp,H.P., Sturani,E. and Klein,R.
  TITLE     A role for the Ras signalling pathway in synaptic transmission and
            long-term memory
  JOURNAL   Nature 390 (6657), 281-286 (1997)
   PUBMED   9384379
REFERENCE   7  (bases 1 to 6294)
  AUTHORS   Farnsworth,C.L., Freshney,N.W., Rosen,L.B., Ghosh,A.,
            Greenberg,M.E. and Feig,L.A.
  TITLE     Calcium activation of Ras mediated by neuronal exchange factor
            Ras-GRF
  JOURNAL   Nature 376 (6540), 524-527 (1995)
   PUBMED   7637786
REFERENCE   8  (bases 1 to 6294)
  AUTHORS   Wei,W., Das,B., Park,W. and Broek,D.
  TITLE     Cloning and analysis of human cDNAs encoding a 140-kDa brain
            guanine nucleotide-exchange factor, Cdc25GEF, which regulates the
            function of Ras
  JOURNAL   Gene 151 (1-2), 279-284 (1994)
   PUBMED   7828890
REFERENCE   9  (bases 1 to 6294)
  AUTHORS   Schweighoffer,F., Faure,M., Fath,I., Chevallier-Multon,M.C.,
            Apiou,F., Dutrillaux,B., Sturani,E., Jacquet,M. and Tocque,B.
  TITLE     Identification of a human guanine nucleotide-releasing factor
            (H-GRF55) specific for Ras proteins
  JOURNAL   Oncogene 8 (6), 1477-1485 (1993)
   PUBMED   7684828
REFERENCE   10 (bases 1 to 6294)
  AUTHORS   Wei,W., Mosteller,R.D., Sanyal,P., Gonzales,E., McKinney,D.,
            Dasgupta,C., Li,P., Liu,B.X. and Broek,D.
  TITLE     Identification of a mammalian gene structurally and functionally
            related to the CDC25 gene of Saccharomyces cerevisiae
  JOURNAL   Proc Natl Acad Sci U S A 89 (15), 7100-7104 (1992)
   PUBMED   1379731
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA126882.1, BC040275.1,
            AK226101.1, AC011944.12 and AL355696.1.
            
            On Feb 27, 2020 this sequence version replaced NM_001145648.2.
            
            Summary: The protein encoded by this gene is a guanine nucleotide
            exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25
            gene product. Functional analysis has demonstrated that this
            protein stimulates the dissociation of GDP from RAS protein. The
            studies of the similar gene in mouse suggested that the Ras-GEF
            activity of this protein in brain can be activated by Ca2+ influx,
            muscarinic receptors, and G protein beta-gamma subunit. Mouse
            studies also indicated that the Ras-GEF signaling pathway mediated
            by this protein may be important for long-term memory.
            Alternatively spliced transcript variants encoding distinct
            isoforms have been reported. [provided by RefSeq, Mar 2009].
            
            Transcript Variant: This variant (3) lacks an in-frame coding exon,
            as compared to variant 1. The resulting isoform (3) lacks an
            internal segment, as compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.1613523.1,
                                           SRR14372080.1179400.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000558480.7/ ENSP00000452781.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                DA126882.1         94-109
            17-3533             BC040275.1         1-3517
            3534-4061           AK226101.1         2911-3438
            4062-5830           AC011944.12        64216-65984
            5831-6249           AL355696.1         681-1099
            6250-6294           AC011944.12        66404-66448
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q25.1"
     gene            1..6294
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /note="Ras protein specific guanine nucleotide releasing
                     factor 1"
                     /db_xref="GeneID:5923"
                     /db_xref="HGNC:HGNC:9875"
                     /db_xref="MIM:606600"
     exon            1..558
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     CDS             283..4056
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /note="isoform 3 is encoded by transcript variant 3;
                     guanine nucleotide-releasing factor, 55 kD; Ras-specific
                     nucleotide exchange factor CDC25; guanine nucleotide
                     exchange factor; Ras-specific guanine nucleotide-releasing
                     factor, CDC25 homolog; ras-specific guanine
                     nucleotide-releasing factor 1; guanine
                     nucleotide-releasing protein; CDC25 like"
                     /codon_start=1
                     /product="ras-specific guanine nucleotide-releasing factor
                     1 isoform 3"
                     /protein_id="NP_001139120.1"
                     /db_xref="CCDS:CCDS45320.1"
                     /db_xref="GeneID:5923"
                     /db_xref="HGNC:HGNC:9875"
                     /db_xref="MIM:606600"
                     /translation="MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWF
                     ALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHE
                     NQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQL
                     RQQIEDGEIEIERLKAEITSLLKDNERIQSTQTVAPNDEDSDIKKIKKVQSFLRGWLC
                     RRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASS
                     KKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQ
                     EFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTL
                     HELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEIL
                     LDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGG
                     KLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVA
                     SSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFS
                     KTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAIVVLDKLITIY
                     KKPISAIPARSLELLFASGQNNKLLYGEPPKSPRATRKFSSPPPLSITKTSSPSRRRK
                     LSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFTNKIPD
                     EGDTTPEKPEDPSALSKQSSEVSMREESDIDQNQSDDGDTETSPTKSPTTPKSVKNKN
                     SSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLAS
                     AGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVIGFLEEVMH
                     DPELLTQERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQL
                     TLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDIN
                     ARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKL
                     QKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRM
                     ISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT"
     exon            559..665
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            666..813
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            814..906
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            907..1160
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1240
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1434
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1435..1544
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1545..1663
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1664..1824
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1825..1888
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            1889..2025
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2026..2108
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2109..2357
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2358..2731
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2732..2857
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            2858..3028
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3029..3135
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3136..3248
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3249..3309
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3310..3413
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3414..3498
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3499..3696
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3697..3776
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3777..3894
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3895..3963
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     exon            3964..6294
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5268..5273
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /note="hexamer: AATAAA"
     polyA_site      5294
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /note="major polyA site"
     regulatory      6268..6273
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
                     /note="hexamer: ATTAAA"
     polyA_site      6294
                     /gene="RASGRF1"
                     /gene_synonym="CDC25; CDC25L; GNRP; GRF1; GRF55; H-GRF55;
                     PP13187; ras-GRF1"
ORIGIN      
        1 agtccctcag tttgcccggc accggaggag ggtcgggcgg catcttccgg gtactggggc
       61 tctgcggagc ggagaagagg ttccagcggg gaatggtata tctggattca agaagccgcg
      121 gctcggcgcc agatcctgga gtgtagatat ttggggggag gggagagcta gagggagcga
      181 gcgagcgagc gccagagaga ggcagcgcgc agcgcgcacg gacggggggc tgctgcgcgg
      241 agaagatgta agcgcgtggg gtctcgctgg cctctgagca ccatgcagaa ggggatccgg
      301 ctgaatgatg gccacgtcgc gtccctggga ctgctggcgc gcaaggacgg cacgcgcaaa
      361 ggctacctga gcaagcggag ttcggacaac acaaaatggc aaaccaagtg gttcgcgctg
      421 ctgcagaacc tgctcttcta cttcgagagc gactcgagct cgcggccctc ggggctttac
      481 ctgctggagg gctgcgtctg cgaccgcgcg ccctccccca agccggcgct gtcggccaag
      541 gagccgctgg agaaacagca ttacttcacg gtgaacttca gccatgagaa ccagaaagcc
      601 ttggagctga ggacagagga cgcaaaagat tgtgacgaat gggtggcagc cattgcacat
      661 gccagctaca ggaccctcgc cacagagcat gaggcattaa tgcagaaata cctgcacctg
      721 ctgcagatcg tggagacaga gaagaccgtg gccaagcagc ttcggcagca gatcgaggat
      781 ggggagatcg agatcgagcg gctgaaggca gagatcacat ccctgctcaa ggacaatgag
      841 cgcatccagt ccacccagac tgtcgccccc aacgatgaag acagcgacat caagaaaatt
      901 aagaaggtgc agagcttcct gcggggctgg ctgtgccggc ggaagtggaa gaccatcatc
      961 caggactaca tccggtcacc ccatgctgac agcatgcgca agaggaacca ggtggtgttc
     1021 agcatgctgg aggctgaggc tgagtacgtg cagcagctgc acatccttgt caacaatttc
     1081 ctgcgcccgc tgcggatggc cgccagctcc aagaagcctc ccatcacaca cgacgacgtc
     1141 agcagcatct tcctgaacag cgaaaccatc atgtttttac atcagatctt ttaccaaggc
     1201 ctgaaggccc gcatctccag ctggcccacg ctggtcctgg ctgacctatt tgacatcctg
     1261 ctgcccatgc tcaacatcta ccaagagttc gtccgcaacc accagtacag cctgcagatc
     1321 ctggcccact gcaagcagaa ccgtgacttc gacaagctgc tgaagcacta cgaggccaag
     1381 cctgactgcg aggagaggac gctggagacc ttcctcacct accccatgtt ccagatcccc
     1441 aggtacatcc tgaccctcca tgagctcctg gcccacacgc ctcatgagca cgttgagcgc
     1501 aacagcctgg actacgccaa gtccaaactg gaggagctgt ccagaataat gcacgatgaa
     1561 gtaagtgaga cggagaacat ccggaaaaac ctggccatcg agcgcatgat catcgaaggc
     1621 tgtgagatcc tcctggacac cagccagacc tttgtgagac aaggttccct cattcaggtg
     1681 cccatgtctg aaaagggcaa gatcaccagg gggcgcctgg ggtctctctc cctaaagaaa
     1741 gagggcgagc gacagtgctt cctgttttct aagcatctga ttatctgtac cagaggctct
     1801 ggagggaagc ttcacttgac caagaatgga gtcatatccc tcattgactg cactttattg
     1861 gaggagccag aaagcacgga ggaggaagcc aaaggatccg gccaagacat agatcacttg
     1921 gattttaaaa tcggggtgga gccaaaggat tccccgccct ttacagtcat cctagtggcc
     1981 tcgtccagac aggagaaggc agcgtggacc agtgacatca gccagtgtgt ggataacatc
     2041 cgatgcaatg ggctcatgat gaacgcattt gaagaaaatt ccaaggtcac tgtgccgcag
     2101 atgatcaagt ccgacgcctc cttatattgt gatgatgttg acattcgctt cagcaaaacc
     2161 atgaactcct gcaaagtgct gcagatccgc tacgccagtg tggagcggct gctggagagg
     2221 ctgacggacc tgcgcttcct gagcatcgac ttcctcaaca ccttcctgca ctcctaccgc
     2281 gtcttcacca ccgccatcgt ggtcctggac aagctcatta ccatctacaa gaagcctatc
     2341 agtgccattc ctgccaggtc gctggagctc ctgtttgcca gtggccagaa caataagctc
     2401 ctgtacggtg aaccccccaa gtccccgcgc gccacccgca agttctcctc gccgccacct
     2461 ctgtccatca ccaagacatc gtcaccgagc cgccggcgga agctctccct gaacatcccc
     2521 atcatcactg gcggcaaggc cctggacctg gccgccctca gctgcaactc caatggctac
     2581 accagcatgt actcggccat gtcacccttc agcaaggcca cgctggacac cagcaagctc
     2641 tatgtgtcca gcagcttcac caacaagatt ccagatgagg gcgatacgac ccctgagaag
     2701 cccgaagacc cttcagcgct cagcaagcag agctcagaag tctccatgag agaggagtca
     2761 gatattgatc aaaaccagag tgatgatggt gatactgaaa catcaccaac taaatctcca
     2821 acaacaccca aatcagtcaa aaacaaaaat tcttcagagt tcccactctt ttcctataac
     2881 aatggagtcg tcatgacctc ctgtcgtgaa ctggacaata accgcagtgc cttgtcggcc
     2941 gcctctgcct ttgccatagc aaccgccggg gccaacgagg gcaccccaaa caaggagaag
     3001 taccggagga tgtccttagc cagtgcaggg tttcccccag accagaggaa tggagacaag
     3061 gagtttgtga tccgcagagc agccaccaat cgtgtcttga acgtgctccg ccactgggtg
     3121 tccaagcact ctcaggactt tgagaccaac gatgagctca aatgcaaggt gatcggcttc
     3181 ctggaagaag tcatgcacga cccggagctc ctgacccagg agcggaaggc tgcagccaac
     3241 atcatcagga ctctgaccca ggaggaccca ggtgacaacc agatcacgct ggaggagatc
     3301 acgcagatgg ctgaaggcgt gaaggctgag ccctttgaaa accactcagc cctggagatc
     3361 gcggagcagc tgaccctgct agatcacctc gtcttcaaga agattcctta tgaggagttc
     3421 ttcggacaag gatggatgaa actggaaaag aatgaaagga ccccttatat catgaaaacc
     3481 actaagcact tcaatgacat cagtaacttg attgcttcag aaatcatccg caatgaggac
     3541 atcaacgcca gggtgagcgc catcgagaag tgggtggccg tagctgacat atgccgctgc
     3601 ctccacaact acaatgccgt actggagatc acctcgtcca tgaaccgcag tgcaatcttc
     3661 cggctcaaaa agacgtggct caaagtctct aagcagacta aagctttgat tgataagctc
     3721 caaaagcttg tgtcatctga gggcagattt aagaatctca gagaagctct gaaaaattgt
     3781 gacccaccct gtgtccctta cctggggatg tacctcaccg acctggcctt catcgaggag
     3841 gggacgccca attacacgga agacggcctg gtcaacttct ccaagatgag gatgatatcc
     3901 catattatcc gagagattcg ccagtttcaa caaactgcct acaaaataga gcaccaagca
     3961 aaggtaacgc aatatttact ggaccaatct tttgtaatgg atgaagaaag cctctacgag
     4021 tcttctctcc gaatagaacc aaaactcccc acctgaagct gagcccagcc cagacccagc
     4081 tgctcccggg gacatgtgct agatgatact gtacatattc gtttggtttc actggatttt
     4141 cttcttcagt atgtgcttct ccaagaatac aaatcgtcct tgttcttaga ttcctgtaga
     4201 accggaatat gaatttctgc accgtttcag acttcgccca cccatccctc ccctcgtctc
     4261 ctgcagtgcc tgtttctttt aaacagctgt actcttggtc cctcttccct agactcctca
     4321 ctcttctcag aggggaaaca gcacccttgc atacaagaat cttagactcc agtcctgctt
     4381 ccgtccctcc ccagcccagg ctcctgggtt gaggtggcca cccaggcatc ctcccagcat
     4441 gttccatgta gttgttataa ctgggtgggg ggtggggggc ggcgggaggg ggaaaccata
     4501 ccctgaagtc cagtcattga caaatcttcc cgctgatggt gatgtcaatt tccaaagcag
     4561 cttctccagc caaaatcgca ggtctcaaaa ctccccatgt gtctcccagt aacctggacg
     4621 gaagggagtc ctctctgctc agaaccacac ccctcgtacc aatgctgcca tctcgttcag
     4681 gccccacgtc gctccttgct ctaggattaa caccagatag catgaatagc agtctcaata
     4741 caactggatg aggccctaga cttcccgagg aaatggagtc acaaacacca agccacgtcg
     4801 actcttgccg accactggcc cagcatccac tgaaagtcgg gaacacaaaa atgtcacttt
     4861 tcctctttaa gctgcctaac ggtcactaca agcttacatt gagatgctct aagtctgtat
     4921 accttgtgat aattagtacc acactgtaga attaggaaat caataaccaa gtgtttttat
     4981 tgcatatact gtagtcctgt ctaaatgcct tctagcagga atatagtaat gtagtgctta
     5041 ttctgtgaat attaccatgt attttttaca ctgtacagta tagaaaacac aggttaactc
     5101 cgaagtggca ggcatgctcc actacaacag acagacaact tttgctgtaa gcaataccta
     5161 gtggtatgag aattctattt caagtcctaa aatatttcaa cttacagctt gtttggaaaa
     5221 aaatttccat ttttgtaaaa atatatgttt cctgattacc tcttgctaat aaatctattc
     5281 tatctcaggg tgaacagcga gttttgatgg gtttcattcg ttcagtcaag gaatatttga
     5341 tgagcacttg ctccttaccc agcccagcct gggaccggag atccagagtg tcaaggacac
     5401 catacctgct ttttaggcac caacagtttg gtggggaaga ctgtttcagt tgcctattgc
     5461 accaagaaca agccactcca aaacataatg gcttaaaacc atttttgtta cctttcacag
     5521 ttctatgggt tgactgggtc cagctgtcag ttcttatttg gagtctctca tgaagtttca
     5581 gtcactcagc aactgggact ggagtcatct gaaggctcaa ctggaccggg tggtgctcta
     5641 gttggtgcac caagatggcg ctgtcacatg gctggatgtt gatgtctgct gggctgcact
     5701 ggggctgttc atgggagcat ccacacgtgg cctctctgtg tggcttgggc tcctcacagt
     5761 ccggaagctg ggttccaaga gcgactgttc cagcagcctc aggcagaagc tacaaggctg
     5821 cccagatgcc atcctgccca gatgtgtcct cactgtcacc caggaaggga agccccggaa
     5881 caccaccctc agtcacaggc atggggccca ctgcaaggca caacccactc cagagctccc
     5941 aaggccgcct cccctgggac ctgcagccag ctggctccat aactgctttt ccgtttcctc
     6001 caggcatttt catcctagcc ctactttgtg ctgggatctg agggggatag caagcggaga
     6061 aagaagggat ctagagggag aagattctag atccttcttt tgactgccag ctgattttga
     6121 ttgccagctt tacctgcttc atgagctcag ccaagtcact taacctctca gagcttcact
     6181 gttctcatct gaaaaatggg gatgaaaatg gtatctcatt acagagtaga attaaatgaa
     6241 ttattataat taatggtatt aagtactatt aaacagttaa tggtaaagaa ggaa
//
