LOCUS       NM_022904               3266 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens RAS protein activator like 3 (RASAL3), transcript
            variant 1, mRNA.
ACCESSION   NM_022904 XM_029084
VERSION     NM_022904.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Lin,Z., Tang,X., Cao,Y., Yang,L., Jiang,M., Li,X., Min,J., Chen,B.,
            Yang,Y. and Gu,C.
  TITLE     CD229 interacts with RASAL3 to activate RAS/ERK pathway in multiple
            myeloma proliferation
  JOURNAL   Aging (Albany NY) 14 (22), 9264-9279 (2022)
   PUBMED   36445333
  REMARK    GeneRIF: CD229 interacts with RASAL3 to activate RAS/ERK pathway in
            multiple myeloma proliferation.
            Erratum:[Aging (Albany NY). 2023 Aug 28;15(16):8528-8529. doi:
            10.18632/aging.205006.. PMID: 37635436]
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 3266)
  AUTHORS   Shin,Y., Kim,Y.W., Kim,H., Shin,N., Kim,T.S., Kwon,T.K., Choi,J.H.
            and Chang,J.S.
  TITLE     RASAL3 preferentially stimulates GTP hydrolysis of the Rho family
            small GTPase Rac2
  JOURNAL   Biomed Rep 9 (3), 241-246 (2018)
   PUBMED   30271600
REFERENCE   4  (bases 1 to 3266)
  AUTHORS   Saito,S., Kawamura,T., Higuchi,M., Kobayashi,T.,
            Yoshita-Takahashi,M., Yamazaki,M., Abe,M., Sakimura,K., Kanda,Y.,
            Kawamura,H., Jiang,S., Naito,M., Yoshizaki,T., Takahashi,M. and
            Fujii,M.
  TITLE     RASAL3, a novel hematopoietic RasGAP protein, regulates the number
            and functions of NKT cells
  JOURNAL   Eur J Immunol 45 (5), 1512-1523 (2015)
   PUBMED   25652366
  REMARK    GeneRIF: identified a novel Ras GTPase-activating proteins protein,
            RASAL3, which is predominantly expressed in cells of hematopoietic
            lineages, including NKT, B, and T cells
REFERENCE   5  (bases 1 to 3266)
  AUTHORS   Buschow,S.I., van Balkom,B.W., Aalberts,M., Heck,A.J., Wauben,M.
            and Stoorvogel,W.
  TITLE     MHC class II-associated proteins in B-cell exosomes and potential
            functional implications for exosome biogenesis
  JOURNAL   Immunol Cell Biol 88 (8), 851-856 (2010)
   PUBMED   20458337
REFERENCE   6  (bases 1 to 3266)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK122587.1 and BC030281.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_022904.2.
            
            Summary: This gene belongs to the Ras GTPase-activating proteins
            (RasGAP) family and encodes a protein with pleckstrin homology
            (PH), C2, and Ras GTPase-activation protein (RasGAP) domains. This
            protein is localized near or at the plasma membrane when expressed
            exogenously. Reduced expression of this gene in some cell lines
            resulted in increased levels of the active form of Ras (Ras-GTP),
            suggesting that this gene may play a role in negatively regulating
            the Ras signaling pathway. Alternative splicing results in multiple
            transcript variants encoding different isoforms. [provided by
            RefSeq, Jan 2017].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK122587.1, SRR1163658.511345.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000343625.12/ ENSP00000341905.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2223              AK122587.1         23-2245
            2224-3266           BC030281.1         1-1043
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.12"
     gene            1..3266
                     /gene="RASAL3"
                     /note="RAS protein activator like 3"
                     /db_xref="GeneID:64926"
                     /db_xref="HGNC:HGNC:26129"
                     /db_xref="MIM:616561"
     exon            1..40
                     /gene="RASAL3"
                     /inference="alignment:Splign:2.1.0"
     exon            41..387
                     /gene="RASAL3"
                     /inference="alignment:Splign:2.1.0"
     CDS             60..3095
                     /gene="RASAL3"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /codon_start=1
                     /product="RAS protein activator like-3 isoform 1"
                     /protein_id="NP_075055.1"
                     /db_xref="CCDS:CCDS46006.1"
                     /db_xref="GeneID:64926"
                     /db_xref="HGNC:HGNC:26129"
                     /db_xref="MIM:616561"
                     /translation="MDPPSPSRTSQTQPTATSPLTSYRWHTGGGGEKAAGGFRWGRFA
                     GWGRALSHQEPMVSTQPAPRSIFRRVLSAPPKESRTSRLRLSKALWGRHKNPPPEPDP
                     EPEQEAPELEPEPELEPPTPQIPEAPTPNVPVWDIGGFTLLDGKLVLLGGEEEGPRRP
                     RVGSASSEGSIHVAMGNFRDPDRMPGKTEPETAGPNQVHNVRGLLKRLKEKKKARLEP
                     RDGPPSALGSRESLATLSELDLGAERDVRIWPLHPSLLGEPHCFQVTWTGGSRCFSCR
                     SAAERDRWIEDLRRQFQPTQDNVEREETWLSVWVHEAKGLPRAAAGAPGVRAELWLDG
                     ALLARTAPRAGPGQLFWAERFHFEALPPARRLSLRLRGLGPGSAVLGRVALALEELDA
                     PRAPAAGLERWFPLLGAPAGAALRARIRARRLRVLPSERYKELAEFLTFHYARLCGAL
                     EPALPAQAKEELAAAMVRVLRATGRAQALVTDLGTAELARCGGREALLFRENTLATKA
                     IDEYMKLVAQDYLQETLGQVVRRLCASTEDCEVDPSKCPASELPEHQARLRNSCEEVF
                     ETIIHSYDWFPAELGIVFSSWREACKERGSEVLGPRLVCASLFLRLLCPAILAPSLFG
                     LAPDHPAPGPARTLTLIAKVIQNLANRAPFGEKEAYMGFMNSFLEEHGPAMQCFLDQV
                     AMVDVDAAPSGYQGSGDLALQLAVLHAQLCTIFAELDQTTRDTLEPLPTILRAIEEGQ
                     PVLVSVPMRLPLPPAQVHSSLSAGEKPGFLAPRDLPKHTPLISKSQSLRSVRRSESWA
                     RPRPDEERPLRRPRPVQRTQSVPVRRPARRRQSAGPWPRPKGSLSMGPAPRARPWTRD
                     SASLPRKPSVPWQRQMDQPQDRNQALGTHRPVNKLAELQCEVAALREEQKVLSRLVES
                     LSTQIRALTEQQEQLRGQLQDLDSRLRAGSSEFDSEHNLTSNEGHSLKNLEHRLNEME
                     RTQAQLRDAVQSLQLSPRTRGSWSQPQPLKAPCLNGDTT"
     exon            388..524
                     /gene="RASAL3"
                     /inference="alignment:Splign:2.1.0"
     exon            525..603
                     /gene="RASAL3"
                     /inference="alignment:Splign:2.1.0"
     exon            604..665
                     /gene="RASAL3"
                     /inference="alignment:Splign:2.1.0"
     exon            666..721
                     /gene="RASAL3"
                     /inference="alignment:Splign:2.1.0"
     exon            722..848
                     /gene="RASAL3"
                     /inference="alignment:Splign:2.1.0"
     exon            849..947
                     /gene="RASAL3"
                     /inference="alignment:Splign:2.1.0"
     exon            948..1490
                     /gene="RASAL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1491..1635
                     /gene="RASAL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1636..1780
                     /gene="RASAL3"
                     /inference="alignment:Splign:2.1.0"
     exon            1781..2017
                     /gene="RASAL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2018..2219
                     /gene="RASAL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2220..2338
                     /gene="RASAL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2339..2729
                     /gene="RASAL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2730..2887
                     /gene="RASAL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2888..2951
                     /gene="RASAL3"
                     /inference="alignment:Splign:2.1.0"
     exon            2952..3266
                     /gene="RASAL3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3243..3248
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RASAL3"
                     /note="hexamer: AGTAAA"
     polyA_site      3266
                     /gene="RASAL3"
                     /note="major polyA site"
ORIGIN      
        1 gcagacggct gctgattctg gggctggtca ggaaaccaag gagacccccc cccccaacca
       61 tggacccacc gtcgccaagc cggacctccc aaacccagcc cacagccacc tctccgctga
      121 cttcctaccg ctggcacaca gggggcggtg gggagaaggc ggctggaggg ttccgctggg
      181 gccgctttgc tggctggggc agggccctga gccaccagga gcccatggtc agcacccagc
      241 cagcccctcg ctcgatattc cgtcgggtcc tatctgcgcc tcccaaggag tcacggacca
      301 gtcgccttcg actctccaag gccctctggg ggaggcataa gaacccaccg ccggagccag
      361 acccggagcc ggagcaggag gccccagagc tggagccgga gccagagctg gagcccccta
      421 ccccacagat ccctgaggcc cccacaccca acgtgcctgt ctgggacatt gggggcttca
      481 ccctgcttga tgggaagctg gtgctgcttg gaggagagga ggagggtcct cgaaggcccc
      541 gggtgggaag tgctagctcc gagggcagca tccacgtggc catggggaac ttcagggatc
      601 cagatcggat gcctggaaaa acagaaccgg agactgctgg tcccaaccag gtccacaacg
      661 ttcgggggtt gctcaagagg ctgaaagaga agaaaaaggc caggttggag ccccgggatg
      721 gaccccccag tgctctgggc tctagggagt cgctggccac actctctgaa ctggacctgg
      781 gtgccgagcg ggatgtgcgg atctggccac tgcaccccag cctcctgggg gagccccact
      841 gctttcaggt aacctggacg ggtggaagcc gctgcttctc ttgtcgctcg gccgctgaga
      901 gagaccgctg gatcgaggac cttcgtcgcc aattccagcc cacccaggac aacgtggagc
      961 gggaagagac atggctgagc gtgtgggtgc acgaagcgaa ggggcttccc cgagcagcgg
     1021 cgggggcacc cggcgtgcgc gccgagctgt ggctggatgg cgcgctgctg gcacgcacgg
     1081 cgcctcgggc cggcccaggc cagctcttct gggccgagcg cttccacttc gaggcgctgc
     1141 caccggcacg tcgcctgtcg ctgcggctgc gcggcttggg cccgggaagc gcggtgctgg
     1201 gccgcgtggc cctggcgctg gaggagctgg acgccccacg cgcgcctgcc gccggtctgg
     1261 agcgctggtt cccgctgctc ggggcgccgg cgggcgcagc gctgcgggcg cggattcggg
     1321 cgcgtcgcct gcgcgtgctg ccgtccgagc gctacaagga gctggcggag ttcctcacct
     1381 tccactatgc gcgcctctgc ggggccctgg agcccgcgct gcctgcgcag gccaaggagg
     1441 agctggcggc agccatggtg cgcgtgctgc gggccaccgg ccgggcgcag gcgctggtga
     1501 ctgacctggg cactgcggag ctggcgcgct gtggaggccg tgaggcgctg ctgttccggg
     1561 aaaacacatt ggccaccaag gctatcgatg agtacatgaa gctcgtggca caggattacc
     1621 tccaggagac cctgggacag gttgtgcggc gtctctgtgc ttctactgag gactgtgaag
     1681 tggaccccag caaatgtcca gcctcggagc tgccagagca ccaggccaga cttcggaaca
     1741 gctgcgagga ggtcttcgaa accattatcc attcctacga ctggttccct gcggagctgg
     1801 gcatcgtgtt ctcaagctgg cgagaagcat gtaaagaacg tggctctgag gtgctgggcc
     1861 cccgactggt gtgcgcctcc ctcttcctgc ggctcctgtg ccctgccatc ctggcaccca
     1921 gcctctttgg tttggcacca gaccatccag cacccggccc agcccgcacc ctcacactga
     1981 ttgccaaggt catccagaac ctcgccaacc gtgccccgtt cggtgagaag gaggcctaca
     2041 tgggcttcat gaatagcttc ctggaggaac atggaccagc catgcaatgc ttcctggacc
     2101 aggtagccat ggtggatgtg gatgctgccc ccagtggtta ccagggcagt ggtgatctgg
     2161 ccctccagtt agctgtcctg catgcccagc tctgtacaat ttttgctgag cttgaccaga
     2221 caacccgaga caccctggaa ccactgccca ccatcctgcg agccattgag gagggccagc
     2281 ctgtgcttgt gtcagtgcca atgcgtctcc cactgccccc ggcccaggtc cactccagcc
     2341 tctccgcagg ggagaagccc ggcttcctgg ccccccggga cctccccaag cacacccctc
     2401 tcatctccaa gagccagtct ctgcgcagcg ttcgccgctc agagagttgg gcccggccac
     2461 ggccggacga agagcggccc ctgcggcggc cccggccggt gcagcgcacg cagagtgtcc
     2521 cggtccggcg tcctgcccgc cgccgccaat ctgcggggcc ctggccgcga cccaaaggct
     2581 ccctgagcat gggaccagcg ccccgcgccc ggccttggac ccgggactcc gcctcgctgc
     2641 ctcggaagcc gtcggtaccc tggcagcgcc aaatggacca gccgcaagac cgaaaccagg
     2701 cactgggcac gcaccgacct gtgaacaagt tggcagagct gcagtgcgag gtggccgctc
     2761 tgcgtgagga gcagaaagtg ctgtcccgcc tcgtggagtc gctgagcacc caaatccggg
     2821 ccttgacgga gcagcaggag cagctgcggg gccagctgca ggatctggac tccaggctcc
     2881 gtgctgggag ctcagagttt gattcagagc acaacctaac aagcaatgaa gggcacagtc
     2941 tgaaaaacct ggagcaccgc ctaaatgaga tggagagaac tcaggctcag ctgagggatg
     3001 ctgtccagag cctgcagctt tctccaagga cgcgggggtc ttggagtcaa ccccagcccc
     3061 tcaaagcacc ctgcctcaat ggagacacca cctgagctgc ccatcctgcc tcatcacacg
     3121 tggtctggga gcagagagat agccatctta gggggggtgt ctgactttgc cttagcccta
     3181 cttggcctac agtggggagt ggagctgctg gtcccaacca ctctggcagt atgaagttgc
     3241 ccagtaaaat cttgatttca gtgaaa
//
