LOCUS       NM_007368               4203 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens RAS p21 protein activator 3 (RASA3), transcript
            variant 1, mRNA.
ACCESSION   NM_007368
VERSION     NM_007368.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Molina-Ortiz,P., Orban,T., Martin,M., Habets,A., Dequiedt,F. and
            Schurmans,S.
  TITLE     Rasa3 controls turnover of endothelial cell adhesion and vascular
            lumen integrity by a Rap1-dependent mechanism
  JOURNAL   PLoS Genet 14 (1), e1007195 (2018)
   PUBMED   29381707
  REMARK    GeneRIF: Rasa3 is a critical regulator of Rap1 in endothelial cells
            which controls adhesions properties and vascular lumen integrity;
            its specific endothelial cell inactivation results in occluded
            blood vessels, hemorrhages and early embryonic death in mouse,
            mimicking thus the Rasa3-/- mouse phenotype.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Battram,A.M., Durrant,T.N., Agbani,E.O., Heesom,K.J., Paul,D.S.,
            Piatt,R., Poole,A.W., Cullen,P.J., Bergmeier,W., Moore,S.F. and
            Hers,I.
  TITLE     The Phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P3) Binder
            Rasa3 Regulates Phosphoinositide 3-kinase (PI3K)-dependent Integrin
            alphaIIbbeta3 Outside-in Signaling
  JOURNAL   J Biol Chem 292 (5), 1691-1704 (2017)
   PUBMED   27903653
  REMARK    GeneRIF: these results support an important role for Rasa3 in
            PI3K-dependent integrin alphaIIbbeta3-mediated outside-in signaling
            and cell spreading.
REFERENCE   3  (bases 1 to 4203)
  AUTHORS   Schurmans,S., Polizzi,S., Scoumanne,A., Sayyed,S. and
            Molina-Ortiz,P.
  TITLE     The Ras/Rap GTPase activating protein RASA3: from gene structure to
            in vivo functions
  JOURNAL   Adv Biol Regul 57, 153-161 (2015)
   PUBMED   25294679
  REMARK    GeneRIF: the function of RASA3 during megakaryopoiesis,
            megakaryocyte adhesion and migration as well as integrin signaling
            has been reported.
            Review article
REFERENCE   4  (bases 1 to 4203)
  AUTHORS   Peters,L.L., Paw,B.H. and Blanc,L.
  TITLE     The scat mouse model highlights RASA3, a GTPase activating protein,
            as a key regulator of vertebrate erythropoiesis and
            megakaryopoiesis
  JOURNAL   Small GTPases 4 (1), 47-50 (2013)
   PUBMED   23221813
REFERENCE   5  (bases 1 to 4203)
  AUTHORS   Kupzig,S., Bouyoucef-Cherchalli,D., Yarwood,S., Sessions,R. and
            Cullen,P.J.
  TITLE     The ability of GAP1IP4BP to function as a Rap1 GTPase-activating
            protein (GAP) requires its Ras GAP-related domain and an arginine
            finger rather than an asparagine thumb
  JOURNAL   Mol Cell Biol 29 (14), 3929-3940 (2009)
   PUBMED   19433443
  REMARK    GeneRIF: model in which the Ras GAP-related domain of GAP1(IP4BP)
            functions to stabilize the switch II region of Rap1, allowing
            stabilization of the transition state during GTP hydrolysis
            initiated by the arginine finger
REFERENCE   6  (bases 1 to 4203)
  AUTHORS   O'Rourke,F., Matthews,E. and Feinstein,M.B.
  TITLE     Isolation of InsP4 and InsP6 binding proteins from human platelets:
            InsP4 promotes Ca2+ efflux from inside-out plasma membrane vesicles
            containing 104 kDa GAP1IP4BP protein
  JOURNAL   Biochem J 315 (Pt 3) (Pt 3), 1027-1034 (1996)
   PUBMED   8645140
REFERENCE   7  (bases 1 to 4203)
  AUTHORS   Cullen,P.J., Hsuan,J.J., Truong,O., Letcher,A.J., Jackson,T.R.,
            Dawson,A.P. and Irvine,R.F.
  TITLE     Identification of a specific Ins(1,3,4,5)P4-binding protein as a
            member of the GAP1 family
  JOURNAL   Nature 376 (6540), 527-530 (1995)
   PUBMED   7637787
  REMARK    GeneRIF: GAP1IP4BP is an inositol 1,3,4,5-tetrakisphosphate
            regulated plasma membrane associated Ras and Rap GAP. Membrane
            association results from its PH domain binding phosphatidylinositol
            4,5-bisphosphate.
REFERENCE   8  (bases 1 to 4203)
  AUTHORS   Briggs,S.D., Bryant,S.S., Jove,R., Sanderson,S.D. and
            Smithgall,T.E.
  TITLE     The Ras GTPase-activating protein (GAP) is an SH3 domain-binding
            protein and substrate for the Src-related tyrosine kinase, Hck
  JOURNAL   J Biol Chem 270 (24), 14718-14724 (1995)
   PUBMED   7782336
REFERENCE   9  (bases 1 to 4203)
  AUTHORS   Hjermstad,S.J., Briggs,S.D. and Smithgall,T.E.
  TITLE     Phosphorylation of the ras GTPase-activating protein (GAP) by the
            p93c-fes protein-tyrosine kinase in vitro and formation of GAP-fes
            complexes via an SH2 domain-dependent mechanism
  JOURNAL   Biochemistry 32 (39), 10519-10525 (1993)
   PUBMED   7691175
REFERENCE   10 (bases 1 to 4203)
  AUTHORS   Hata,Y., Kikuchi,A., Sasaki,T., Schaber,M.D., Gibbs,J.B. and
            Takai,Y.
  TITLE     Inhibition of the ras p21 GTPase-activating protein-stimulated
            GTPase activity of c-Ha-ras p21 by smg p21 having the same putative
            effector domain as ras p21s
  JOURNAL   J Biol Chem 265 (13), 7104-7107 (1990)
   PUBMED   2158984
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA328760.1, BC038456.1,
            AK128094.1 and BC047242.1.
            
            On Nov 22, 2018 this sequence version replaced NM_007368.3.
            
            Summary: This gene encodes a protein that binds inositol
            1,3,4,5-tetrakisphosphate and stimulates the GTPase activity of Ras
            p21. This protein functions as a negative regulator of the Ras
            signalling pathway. It is localized to the cell membrane via a
            pleckstrin homology (PH) domain in the C-terminal region.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Mar 2016].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC047242.1, SRR14038192.1238340.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000334062.8/ ENSP00000335029.7
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3                 DA328760.1         1-3
            4-1459              BC038456.1         1-1456
            1460-3012           AK128094.1         1895-3447
            3013-4203           BC047242.1         3001-4191
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q34"
     gene            1..4203
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /note="RAS p21 protein activator 3"
                     /db_xref="GeneID:22821"
                     /db_xref="HGNC:HGNC:20331"
                     /db_xref="MIM:605182"
     exon            1..189
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     CDS             135..2639
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /note="isoform 1 is encoded by transcript variant 1;
                     Ins(1,3,4,5)P4-binding protein; GTPase activating protein
                     III; ras GTPase-activating protein 3; ins P4-binding
                     protein; GTPase-activating protein 1 family, inositol
                     1,3,4,5-tetrakisphosphate-binding protein"
                     /codon_start=1
                     /product="ras GTPase-activating protein 3 isoform 1"
                     /protein_id="NP_031394.2"
                     /db_xref="CCDS:CCDS32016.1"
                     /db_xref="GeneID:22821"
                     /db_xref="HGNC:HGNC:20331"
                     /db_xref="MIM:605182"
                     /translation="MAVEDEGLRVFQSVKIKIGEAKNLPSYPGPSKMRDCYCTVNLDQ
                     EEVFRTKIVEKSLCPFYGEDFYCEIPRSFRHLSFYIFDRDVFRRDSIIGKVAIQKEDL
                     QKYHNRDTWFQLQHVDADSEVQGKVHLELRLSEVITDTGVVCHKLATRIVECQGLPIV
                     NGQCDPYATVTLAGPFRSEAKKTKVKRKTNNPQFDEVFYFEVTRPCSYSKKSHFDFEE
                     EDVDKLEIRVDLWNASNLKFGDEFLGELRIPLKVLRQSSSYEAWYFLQPRDNGSKSLK
                     PDDLGSLRLNVVYTEDHVFSSDYYSPLRDLLLKSADVEPVSASAAHILGEVCREKQEA
                     AVPLVRLFLHYGRVVPFISAIASAEVKRTQDPNTIFRGNSLASKCIDETMKLAGMHYL
                     HVTLKPAIEEICQSHKPCEIDPVKLKDGENLENNMENLRQYVDRVFHAITESGVSCPT
                     VMCDIFFSLREAAAKRFQDDPDVRYTAVSSFIFLRFFAPAILSPNLFQLTPHHTDPQT
                     SRTLTLISKTVQTLGSLSKSKSASFKESYMATFYEFFNEQKYADAVKNFLDLISSSGR
                     RDPKSVEQPIVLKEGFMIKRAQGRKRFGMKNFKKRWFRLTNHEFTYHKSKGDQPLYSI
                     PIENILAVEKLEEESFKMKNMFQVIQPERALYIQANNCVEAKDWIDILTKVSQCNQKR
                     LTVYHPSAYLSGHWLCCRAPSDSAPGCSPCTGGLPANIQLDIDGDRETERIYSLFNLY
                     MSKLEKMQEACGSKSVYDGPEQEEYSTFVIDDPQETYKTLKQVIAGVGALEQEHAQYK
                     RDKFKKTKYGSQEHPIGDKSFQNYIRQQSETSTHSI"
     exon            190..307
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            308..411
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            412..506
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            507..583
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            584..664
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            665..737
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            738..814
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            815..919
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1076
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1077..1225
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1340
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1415
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1416..1539
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1540..1646
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..1724
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1725..1802
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1803..1876
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1877..1983
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            1984..2066
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            2067..2275
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            2276..2379
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            2380..2563
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     exon            2564..4203
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4176..4181
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /note="hexamer: ATTAAA"
     polyA_site      4203
                     /gene="RASA3"
                     /gene_synonym="GAP1IP4BP; GAPIII"
                     /note="major polyA site"
ORIGIN      
        1 aactggcggt cttgcaggcg ggcggaccct cggccgcgct cggggctccg gcgccgccgc
       61 tcctcctgct cggcctgagc tgcccgggct cggcgcgcgc ttggggcgag gctcggcggg
      121 cgcggacgcg cagcatggcg gtggaggacg aggggctccg ggtcttccag agcgtgaaga
      181 tcaagatcgg tgaagccaaa aaccttccct cttacccggg gccgagcaag atgagggatt
      241 gctactgcac ggtgaacctg gaccaggagg aggttttcag gaccaaaatt gtggaaaagt
      301 cactctgccc gttttacgga gaagactttt actgtgaaat tcctcggagc tttcgtcacc
      361 tgtccttcta cattttcgat agagacgttt tccggaggga ttccatcata gggaaggtgg
      421 ccatccagaa ggaggacttg cagaagtacc acaacaggga cacctggttc cagctgcagc
      481 acgtggacgc tgactcggaa gtgcagggca aagtgcacct ggagctgcgg ctgagcgagg
      541 tcatcacaga cactggggtc gtctgccaca agctcgccac acgcatcgtc gagtgccagg
      601 gcctccccat cgtgaatggg caatgtgacc cctacgccac cgtgacgctg gcaggaccct
      661 tcagatcaga agcaaagaag acgaaagtga agaggaagac caacaatccc cagttcgatg
      721 aagtgtttta ttttgaggtg acccggccct gtagctacag caagaagtcc cactttgact
      781 ttgaggagga agacgtggac aagctcgaaa tcagagttga cctctggaat gccagtaacc
      841 tgaagtttgg agatgaattc ctgggagaac taaggatccc gttgaaagtc ctgcggcagt
      901 ccagctccta cgaggcgtgg tacttcctcc agccccggga caatggtagc aagagcctaa
      961 agccagacga cctgggctcc ctgcggctga acgtggtata cacggaagac cacgtgtttt
     1021 cttctgacta ttacagccct ctgcgggacc tgctgttgaa gtctgcggat gtggagcccg
     1081 tgtcagcgtc tgcggcccac atcctgggcg aggtttgccg ggagaagcag gaggcggccg
     1141 tcccgctggt gcggctcttc ctacactatg gcagggtggt gccattcatc agtgccatcg
     1201 ccagcgcgga ggtgaagcgg acccaggacc ccaacaccat cttccgagga aactcactgg
     1261 cgtccaagtg catcgacgag accatgaagc tggcggggat gcattacctg catgtcaccc
     1321 tgaagcccgc catcgaggag atatgccaga gccacaaacc ctgtgaaatc gaccctgtga
     1381 agttgaaaga cggagaaaac cttgaaaaca acatggagaa cctacggcag tatgtggacc
     1441 gcgtcttcca cgccatcact gagtctgggg tgagctgccc gaccgtcatg tgtgacatct
     1501 tcttctccct ccgggaggcg gcggccaagc gcttccagga tgacccggac gtcaggtaca
     1561 ctgcagtgag cagcttcatc ttcctgaggt tctttgcgcc cgccattctc tcccccaacc
     1621 tcttccagct cacgccgcac cacacggacc cccagacgtc caggacgctg acattgatct
     1681 ccaagaccgt tcagaccctc ggcagcctgt ccaagtccaa atctgcgagt tttaaggagt
     1741 cctacatggc tacattttat gaattcttca atgagcagaa atatgctgat gcggtgaaga
     1801 acttcttgga tctgatttcg tcctcgggga gaagagaccc caagagtgtt gagcagccca
     1861 tcgtgcttaa agaagggttc atgatcaaga gggcccaagg acggaagcgc tttgggatga
     1921 agaattttaa gaagagatgg tttcgcttga ccaaccatga atttacctac cacaaaagca
     1981 aaggggacca gcctctctac agcattccca tcgagaacat cctggcagtg gagaagctgg
     2041 aggaggagtc tttcaaaatg aaaaacatgt tccaggtcat ccagccagag cgtgcgctgt
     2101 acatccaggc caacaactgc gtggaggcca aggactggat cgacattctc accaaagtga
     2161 gccagtgcaa ccagaagcgc ctcaccgtct accacccgtc cgcctacctg agcggccact
     2221 ggctgtgctg tagggcgcca tccgactcgg ctccgggctg ctcgccctgc actggcggcc
     2281 tcccagccaa catccagctg gacattgatg gggaccgtga gacggagcgt atctactccc
     2341 tcttcaactt gtacatgagc aagctggaga agatgcagga ggcctgtggg agcaaatctg
     2401 tgtatgacgg cccggagcag gaggagtatt cgacgttcgt cattgacgac ccccaggaga
     2461 cctacaagac gctaaagcaa gtcatcgctg gggttggggc tttggagcag gagcacgccc
     2521 agtataagag ggacaagttc aagaagacga aatatggaag ccaggagcac cccatcggag
     2581 acaagagctt ccagaactac atccggcagc agtccgagac ctccactcat tccatttaaa
     2641 gtctgcggga cgcgcccagt ggcgccagca agctgcccat gcaaagccgc agcctttggg
     2701 agggagaaga gaaggaagag cgcagagccg gcaaagtgag atgcacgccg tcccggccca
     2761 tagacaagtg gcgctggggt ttccctctcc cagaaccgct gccaccaccg ccacgccctc
     2821 cgcagaaagc ccacctggcc ctgtggaacg tgtcgtcatt tgattctgtg ctttttgctc
     2881 cttgtttgtg gggatctggg gatcagctga aggatcagga agtgtgggct gtgccctagc
     2941 cacaccacag ccagcctcag ggagccacgg cccgtctagt cgactgtgac atgcacctcc
     3001 ggccgtgtgt gcgtcagtct cgtgcacgtc tgtcttgcgt gcacggtgcg tgtgacgctg
     3061 ggctctgtgg ctctgtgccc gggaggcctg ctggccccgc ctcggctttc ggcttcatca
     3121 cattgggagg ttcagagcat tactctccca ctgtgcctgc catccaggca gccatgggag
     3181 gcggcctccc tgttccactt tcgaggttcg tttgattcct ggctgagggg tcagttttat
     3241 ggctgggagg tgcagaatta cacagaagtc ccagtctgag gatgtccttg gtgcttgggg
     3301 gatacgggtt ctgtccacag agtcccttgg agggaaaggt gtgggctcca gcttctccag
     3361 gcgtctgcgg agcccacagt tgaagcccac atcgttttgc tgttgaatgg ggtttaaaat
     3421 cagaattaac atttgccacc ccccgtggaa gtttgaagat tttaaaaata gaaatgtaca
     3481 tttttcaagc tgtttttctt tatgtttttg aaggaccatt tttaattagc tctttgatac
     3541 aaagtaactc agaacgtcaa aacctatacc cactaaaggg aaggctgccg ggaaggcaaa
     3601 tggaacagga atggagcctg tctcaggaag gccagctgca ggtcctccag aaaatcaaag
     3661 aagggaagaa actctgagtt tgaggtacag gggcttcggg gtgcagacgt ccctccaggg
     3721 cccatggtca gtattgcacc tgtgttatga acccccagat gctgtgcagg gcaggggcgg
     3781 gggctgctgt tttattgggg aggggagcat cctaaaaatg gggtccaggc agacccctcc
     3841 agacctcaca ctgccgagga ggcctttccc aaagggcgtc ctcccgggat gcagacggca
     3901 ggtgtgtggg aagcgccgtt taaatacaca gcacgacgta tccttgtacc gacttctccc
     3961 ggttcttgtt tgaaaatact gtagttcagg ctcttgatct agatggcaga taggaacttt
     4021 cttgtcacaa aaatactgga ggaaaatgtt gtaaaaatag acttttggac acacagctgt
     4081 tggggctgca ctgagctgca atttttaaca tggatttata acttaatgtt tctgtttata
     4141 aaatactaat gatttgcaat gtattttact ggccaattaa aacagatgtt ttattctttc
     4201 tga
//
