LOCUS       NM_002890               4746 bp    mRNA    linear   PRI 10-OCT-2024
DEFINITION  Homo sapiens RAS p21 protein activator 1 (RASA1), transcript
            variant 1, mRNA.
ACCESSION   NM_002890
VERSION     NM_002890.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Ouyang,H., Wu,S., Li,W., Grey,M.J., Wu,W. and Hansen,S.H.
  TITLE     p120 RasGAP and ZO-2 are essential for Hippo signaling and
            tumor-suppressor function mediated by p190A RhoGAP
  JOURNAL   Cell Rep 42 (12), 113486 (2023)
   PUBMED   37995182
  REMARK    GeneRIF: p120 RasGAP and ZO-2 are essential for Hippo signaling and
            tumor-suppressor function mediated by p190A RhoGAP.
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Mologousis,M.A., Ostertag-Hill,C.A., Haimes,H., Fishman,S.J.,
            Mulliken,J.B. and Liang,M.G.
  TITLE     Spectrum of lymphatic anomalies in patients with RASA1-related
            CM-AVM
  JOURNAL   Pediatr Dermatol 40 (6), 1028-1034 (2023)
   PUBMED   37767822
  REMARK    GeneRIF: Spectrum of lymphatic anomalies in patients with
            RASA1-related CM-AVM.
REFERENCE   3  (bases 1 to 4746)
  AUTHORS   Vish,K.J., Stiegler,A.L. and Boggon,T.J.
  TITLE     Diverse p120RasGAP interactions with doubly phosphorylated partners
            EphB4, p190RhoGAP, and Dok1
  JOURNAL   J Biol Chem 299 (9), 105098 (2023)
   PUBMED   37507023
  REMARK    GeneRIF: Diverse p120RasGAP interactions with doubly phosphorylated
            partners EphB4, p190RhoGAP, and Dok1.
REFERENCE   4  (bases 1 to 4746)
  AUTHORS   Li,X., Ma,M., Liu,R., Yang,Y. and Yue,W.
  TITLE     Three Cases of Familial Moyamoya Disease with RASA1 Mutations-A
            Case Report
  JOURNAL   World Neurosurg 167, e972-e977 (2022)
   PUBMED   36064119
  REMARK    GeneRIF: Three Cases of Familial Moyamoya Disease with RASA1
            Mutations-A Case Report.
REFERENCE   5  (bases 1 to 4746)
  AUTHORS   Wei,L., Gu,W., Hu,L., Wang,K., Huang,H. and Shen,Y.
  TITLE     Regulation of IncRNA ZNF667-AS1 in Proliferation and Invasion of
            Esophageal Squamous Cell Carcinoma Cells via Mediating ceRNA
            Network
  JOURNAL   Crit Rev Eukaryot Gene Expr 32 (6), 57-68 (2022)
   PUBMED   35997118
  REMARK    GeneRIF: Regulation of IncRNA ZNF667-AS1 in Proliferation and
            Invasion of Esophageal Squamous Cell Carcinoma Cells via Mediating
            ceRNA Network.
REFERENCE   6  (bases 1 to 4746)
  AUTHORS   Bayrak-Toydemir,P. and Stevenson,D.A.
  TITLE     Capillary Malformation-Arteriovenous Malformation Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   21348050
REFERENCE   7  (bases 1 to 4746)
  AUTHORS   Sjolander,A. and Lapetina,E.G.
  TITLE     Agonist-induced association of the p21ras GTPase-activating protein
            with phosphatidylinositol 3-kinase
  JOURNAL   Biochem Biophys Res Commun 189 (3), 1503-1508 (1992)
   PUBMED   1336372
REFERENCE   8  (bases 1 to 4746)
  AUTHORS   Pronk,G.J., Medema,R.H., Burgering,B.M., Clark,R., McCormick,F. and
            Bos,J.L.
  TITLE     Interaction between the p21ras GTPase activating protein and the
            insulin receptor
  JOURNAL   J Biol Chem 267 (33), 24058-24063 (1992)
   PUBMED   1331107
REFERENCE   9  (bases 1 to 4746)
  AUTHORS   Kashishian,A., Kazlauskas,A. and Cooper,J.A.
  TITLE     Phosphorylation sites in the PDGF receptor with different
            specificities for binding GAP and PI3 kinase in vivo
  JOURNAL   EMBO J 11 (4), 1373-1382 (1992)
   PUBMED   1314164
  REMARK    Erratum:[EMBO J 1992 Oct;11(10):3809]
REFERENCE   10 (bases 1 to 4746)
  AUTHORS   Ikawa,S. and Weinberg,R.A.
  TITLE     An interaction between p21ras and heat shock protein hsp60, a
            chaperonin
  JOURNAL   Proc Natl Acad Sci U S A 89 (6), 2012-2016 (1992)
   PUBMED   1347942
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC126776.2, AB209106.1 and
            M23379.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_002890.2.
            
            Summary: The protein encoded by this gene is located in the
            cytoplasm and is part of the GAP1 family of GTPase-activating
            proteins. The gene product stimulates the GTPase activity of normal
            RAS p21 but not its oncogenic counterpart. Acting as a suppressor
            of RAS function, the protein enhances the weak intrinsic GTPase
            activity of RAS proteins resulting in the inactive GDP-bound form
            of RAS, thereby allowing control of cellular proliferation and
            differentiation. Mutations leading to changes in the binding sites
            of either protein are associated with basal cell carcinomas.
            Mutations also have been associated with hereditary capillary
            malformations (CM) with or without arteriovenous malformations
            (AVM) and Parkes Weber syndrome. Alternative splicing results in
            two isoforms where the shorter isoform, lacking the N-terminal
            hydrophobic region but retaining the same activity, appears to be
            abundantly expressed in placental but not adult tissues. [provided
            by RefSeq, May 2012].
            
            Transcript Variant: This variant (1) lacks the alternatively
            spliced insert found in variant 2, resulting in translation of the
            longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC033015.2, M23379.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000274376.11/ ENSP00000274376.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-370               AC126776.2         15205-15574
            371-451             AB209106.1         1-81
            452-4746            M23379.1           1-4295
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q14.3"
     gene            1..4746
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /note="RAS p21 protein activator 1"
                     /db_xref="GeneID:5921"
                     /db_xref="HGNC:HGNC:9871"
                     /db_xref="MIM:139150"
     exon            1..1108
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             570..3713
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /note="isoform 1 is encoded by transcript variant 1;
                     triphosphatase-activating protein; RAS p21 protein
                     activator (GTPase activating protein) 1; p120 RAS GTPase
                     activating protein"
                     /codon_start=1
                     /product="ras GTPase-activating protein 1 isoform 1"
                     /protein_id="NP_002881.1"
                     /db_xref="CCDS:CCDS34200.1"
                     /db_xref="GeneID:5921"
                     /db_xref="HGNC:HGNC:9871"
                     /db_xref="MIM:139150"
                     /translation="MMAAEAGSEEGGPVTAGAGGGGAAAGSSAYPAVCRVKIPAALPV
                     AAAPYPGLVETGVAGTLGGGAALGSEFLGAGSVAGALGGAGLTGGGTAAGVAGAAAGV
                     AGAAVAGPSGDMALTKLPTSLLAETLGPGGGFPPLPPPPYLPPLGAGLGTVDEGDSLD
                     GPEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEERLRQAGKSGSYLIRESDRRPGSFVL
                     SFLSQMNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPE
                     PVEDRRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVED
                     LVEEVGREEDPHEGKIWFHGKISKQEAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRT
                     NENIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYRKEQIVEGYYLKEPVPMQDQEQVL
                     NDTVDGKEIYNTIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLYFILEGSDAQLIYFE
                     SEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGETPEQAE
                     DWMKGLQAFCNLRKSSPGTSNKRLRQVSSLVLHIEEAHKLPVKHFTNPYCNIYLNSVQ
                     VAKTHAREGQNPVWSEEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQK
                     GHATDEWFLLSSHIPLKGIEPGSLRVRARYSMEKIMPEEEYSEFKELILQKELHVVYA
                     LSHVCGQDRTLLASILLRIFLHEKLESLLLCTLNDREISMEDEATTLFRATTLASTLM
                     EQYMKATATQFVHHALKDSILKIMESKQSCELSPSKLEKNEDVNTNLTHLLNILSELV
                     EKIFMASEILPPTLRYIYGCLQKSVQHKWPTNTTMRTRVVSGFVFLRLICPAILNPRM
                     FNIISDSPSPIAARTLILVAKSVQNLANLVEFGAKEPYMEGVNPFIKSNKHRMIMFLD
                     ELGNVPELPDTTEHSRTDLSRDLAALHEICVAHSDELRTLSNERGAQQHVLKKLLAIT
                     ELLQQKQNQYTKTNDVR"
     exon            1109..1261
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1262..1397
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1398..1468
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1586
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1587..1618
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1619..1671
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1672..1822
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1823..1901
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1902..2022
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2023..2179
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2180..2267
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2268..2345
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2346..2503
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2504..2580
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2581..2753
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2754..2913
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2914..3056
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3057..3172
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3173..3259
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3260..3327
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3328..3416
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3417..3494
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3495..3629
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3630..4746
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4723..4728
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /note="hexamer: AATAAA"
     polyA_site      4746
                     /gene="RASA1"
                     /gene_synonym="CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP;
                     p120RASGAP; PKWS; RASA; RASGAP"
                     /note="major polyA site"
ORIGIN      
        1 actcactgag agctccaggt agtgagcagt tcagtcgatt tcctcgttac cccgcccccc
       61 tttctcttgc ccccccaccc ctctcatctg cctggtggag gatgaagcgg ctgcagtggc
      121 cccagcctca gcagcggcac cggcggtggc tgcggtgtgg gtggccggaa ctggggtggt
      181 gaagaagcgg tggtggcggc tgaaggagcg gtagcagcct cagcctcttt ccctgtgctt
      241 cctttcctct ttagtgcagc gaggaagttt tcgcttctgt acatgtgttt gtgtgcgtga
      301 gtgtgggtgt gtgcggtgag gtttgggtgg cgtttgtgca ggcgttgggt tttttgccca
      361 cttggcttcc cgtaacccag gcagctgggg agcctgggct gtggccctag gagggggcgc
      421 ggcggcgggc tctctccttt tgttgttgtt tcctcagcct ggggagctga aggggagacg
      481 cgtctgggtg gggctgctcg gagcccgggc ctggtggccc ctggggctcc cgggcgggca
      541 gggtagggca gagtagagcg ggcttcaaca tgatggcggc cgaggccggc agtgaggagg
      601 gcggcccggt aacagccgga gctggaggag gcggcgcggc agcgggctcc agtgcctatc
      661 ccgcagtgtg tcgggtgaag atacccgcgg ccctgcctgt ggcagccgcc ccctatcctg
      721 ggctggtgga gaccggagtg gctggaactc tgggtggcgg agccgctttg gggtcagagt
      781 tcctaggagc cgggtctgtg gcaggggcac tggggggagc tggactgaca gggggaggta
      841 ctgctgctgg cgtagctggt gctgctgctg gcgtggccgg tgctgctgtt gctggaccta
      901 gtggagacat ggctctcacc aaactgccca cttcgttgct tgctgagact ctcgggccag
      961 gcggcggttt tccccctctg ccccctcccc cttacctgcc ccctttgggg gcgggcctcg
     1021 ggacagtgga cgaaggtgac tctctggatg gaccagaata cgaggaggaa gaggtggcca
     1081 taccgttgac cgctcctcca actaaccagt ggtatcacgg aaaacttgac agaacgatag
     1141 cagaagaacg cctcaggcag gcagggaagt ctggcagtta tcttataaga gagagtgatc
     1201 ggaggccagg gtcctttgta ctttcatttc ttagccagat gaatgttgtc aaccatttta
     1261 ggattattgc tatgtgtgga gattactaca ttggtggaag acgtttttct tcactgtcag
     1321 acctaatagg ttattacagt catgtttctt gtttgcttaa aggagaaaaa ttactttacc
     1381 cagttgcacc accagagcca gtagaagata gaaggcgtgt acgagctatt ctaccttaca
     1441 caaaagtacc agacactgat gaaataagtt tcttaaaagg agatatgttc attgttcata
     1501 atgaattaga agatggatgg atgtgggtta caaatttaag aacagatgaa caaggcctta
     1561 ttgttgaaga cctagtagaa gaggtgggcc gggaagaaga tccacatgaa ggaaaaatat
     1621 ggttccatgg gaagatttcc aaacaggaag cttataattt actaatgaca gttggtcaag
     1681 tctgcagttt tcttgtgagg ccctcagata atactcctgg cgattattca ctttatttcc
     1741 ggaccaatga aaatattcag cgatttaaaa tatgtccaac gccaaacaat cagtttatga
     1801 tgggaggccg gtattataac agcattgggg acatcataga tcactatcga aaagaacaga
     1861 ttgttgaagg atattatctt aaggaacctg taccaatgca ggatcaagaa caagtactca
     1921 atgacacagt ggatggcaag gaaatctata ataccatccg tcgtaaaaca aaggatgcct
     1981 tttataaaaa cattgttaag aaaggttatc ttctgaaaaa gggcaaagga aaacgttgga
     2041 aaaatttata ttttatctta gagggtagtg atgcccaact tatttatttt gaaagcgaaa
     2101 aacgagctac caaaccaaaa ggattaatag atctcagtgt atgttctgtc tatgtcgttc
     2161 atgatagtct ctttggcagg ccaaactgtt ttcagatagt agttcagcac tttagtgaag
     2221 aacattacat cttttacttt gcaggagaaa ctccagaaca agcagaggat tggatgaaag
     2281 gtctgcaggc attttgcaat ttacggaaaa gtagtccagg gacatccaat aaacgccttc
     2341 gtcaggtcag cagccttgtt ttacatattg aagaagccca taaactccca gtaaaacatt
     2401 ttactaatcc atattgtaac atctacctga atagtgtcca agtagcaaaa actcatgcaa
     2461 gggaagggca aaacccagta tggtcagaag agtttgtctt tgatgatctt cctcctgaca
     2521 tcaatagatt tgaaataact cttagtaata aaacaaagaa aagcaaagat cctgatatct
     2581 tatttatgcg ctgccagttg agccgattac agaaagggca tgccacagat gaatggtttc
     2641 tgctcagctc ccatatacca ttaaaaggta ttgaaccagg gtccctgcgt gttcgagcac
     2701 gatactctat ggaaaaaatc atgccagaag aagagtacag tgaatttaaa gagcttatac
     2761 tgcaaaagga acttcatgta gtctatgctt tatcacatgt atgtggacaa gaccgaacac
     2821 tactggccag catcctactg aggatttttc ttcacgaaaa gcttgaatcg ttgttgttat
     2881 gcacactaaa tgacagagaa ataagcatgg aagatgaagc cactacccta tttcgagcca
     2941 caacacttgc aagcaccttg atggagcagt atatgaaagc cactgctaca cagtttgttc
     3001 atcatgcttt gaaagactct attttaaaga taatggaaag caagcagtct tgtgagttaa
     3061 gtccatcaaa gttagaaaaa aatgaagatg tgaacactaa tttaacacac ctattgaaca
     3121 tactttcaga gcttgtggag aaaatattca tggcttcaga aatacttcca ccgacattga
     3181 gatatattta tgggtgttta cagaaatctg ttcagcataa gtggcctaca aataccacca
     3241 tgagaacaag agttgttagt ggttttgttt ttcttcgact catctgtcct gccatcctga
     3301 atccacggat gttcaatatc atctcagatt ctccatctcc tattgctgca agaacactga
     3361 tattagtggc taaatctgtg cagaacttag caaatcttgt ggaatttgga gctaaggagc
     3421 cctacatgga aggtgtcaat ccattcatca aaagcaacaa acatcgtatg atcatgtttt
     3481 tagatgaact tgggaatgta cctgaacttc cggacactac agagcattct agaacggacc
     3541 tgtcccgtga tttagcagca ttgcatgaga tttgcgtggc tcattcagat gaacttcgaa
     3601 cgctcagtaa tgagcgtggt gcacagcagc acgtattgaa aaagcttctg gctataacag
     3661 aactgcttca acaaaaacaa aaccagtata caaaaaccaa tgatgtcagg tagcagcctt
     3721 cgccccagtg ttctgcatgg attcagcatg tccaacatgg taattcactt cagtttaatg
     3781 tctcctttgc tcttgccaaa aaatagcaca cttttccaca ttccagtgat gtgtgagcta
     3841 tgcaaacaaa atccaagatt ctgctggtga ataactatgc cagcaacctt gtaagctatc
     3901 tgtgcaggat atttgcacta tttccacatg gaatcaatct ttaacaacct ctgagccttg
     3961 gtgtacagac cacctttcac aaaacgaaat gctatgactg tatcttgata tctcgaactt
     4021 tcaaaatata ttttcagtac acccagttgc caaagttttg ctgtctctta gagaaagaac
     4081 tatgaaatca actgacaaga aacacattct tattgacaat tgtgtataac tggattgcag
     4141 actgttctta ctgtaactac ttcctgatta ggaatatgac catttgactg ttcaatgatt
     4201 atttgtattt acagtttcca gagtttgtca ttataatagg aacaatcttt gctgtatact
     4261 tttaaaaaat actctgctat ttctcttgct ggaactgttg aaagaaaata tatagaatga
     4321 tctattgctc atcagcttta ttttttaaac atacgactta ttttgttgaa attgtcaaag
     4381 actgtattta gatctcataa tgctttgtta aatgtttaca agtaaatagt ttgaattcag
     4441 taaatattat tggttgttgt attgatcaat gcatgttacc cattcaacca ttttatagac
     4501 taccaatttc ttttatgtta actagaatgc ttttgttaaa agttatttgt tcattatttg
     4561 tgctacccct ttgattatgc agacaacctc atcagctgcc taacttatcc atctttgaac
     4621 ttctgactac ttgttgtatc tgctggatat ttagttcaac tgtatagttt tatttacttc
     4681 tgtatgtgta tttttgtgaa gtattcacaa aggttaagtt aaaataaaac caagggatat
     4741 cttgca
//
