LOCUS       NM_213589               9699 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens Ras association (RalGDS/AF-6) and pleckstrin homology
            domains 1 (RAPH1), transcript variant 1, mRNA.
ACCESSION   NM_213589
VERSION     NM_213589.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9699)
  AUTHORS   Liu,Q., Cao,Y., Wei,X., Dong,H., Cui,M., Guan,S., Liu,B., Wang,X.
            and Xing,P.
  TITLE     Nuclear isoform of RAPH1 interacts with FOXQ1 to promote
            aggressiveness and radioresistance in breast cancer
  JOURNAL   Cell Death Dis 14 (12), 803 (2023)
   PUBMED   38062011
  REMARK    GeneRIF: Nuclear isoform of RAPH1 interacts with FOXQ1 to promote
            aggressiveness and radioresistance in breast cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9699)
  AUTHORS   Kurozumi,S., Joseph,C., Sonbul,S., Aleskandarany,M.A., Pigera,M.,
            Alsaleem,M., Alsaeed,S., Kariri,Y., Nolan,C.C., Diez-Rodriguez,M.,
            Johnston,S., Mongan,N.P., Fujii,T., Shirabe,K., Martin,S.G.,
            Ellis,I.O., Green,A.R. and Rakha,E.A.
  TITLE     Clinicopathological and prognostic significance of Ras association
            and pleckstrin homology domains 1 (RAPH1) in breast cancer
  JOURNAL   Breast Cancer Res Treat 172 (1), 61-68 (2018)
   PUBMED   30056565
  REMARK    GeneRIF: High RAPH1 expression is correlated with aggressive breast
            cancer phenotypes and provides independent prognostic value in
            invasive breast cancer.
REFERENCE   3  (bases 1 to 9699)
  AUTHORS   A De Andrade,F., Batistela,M.S., Amaral Sda,C., Dos Santos,W.,
            Mikami,L.R., Chautard-Freire-Maia,E.A., Furtado-Alle,L. and
            Souza,R.L.
  TITLE     Association between RAPH1 Gene Haplotypes and CHE2 Locus Phenotypes
  JOURNAL   Ann Hum Genet 80 (4), 203-209 (2016)
   PUBMED   27346732
  REMARK    GeneRIF: The human RAPH1 gene is the CHE2 locus.
REFERENCE   4  (bases 1 to 9699)
  AUTHORS   Lagarrigue,F., Vikas Anekal,P., Lee,H.S., Bachir,A.I., Ablack,J.N.,
            Horwitz,A.F. and Ginsberg,M.H.
  TITLE     A RIAM/lamellipodin-talin-integrin complex forms the tip of sticky
            fingers that guide cell migration
  JOURNAL   Nat Commun 6, 8492 (2015)
   PUBMED   26419705
  REMARK    GeneRIF: Disruption of the RIAM/lamellipodin-integrin-talin complex
            markedly impairs cell migration.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9699)
  AUTHORS   Hansen,S.D. and Mullins,R.D.
  TITLE     Lamellipodin promotes actin assembly by clustering Ena/VASP
            proteins and tethering them to actin filaments
  JOURNAL   Elife 4, e06585 (2015)
   PUBMED   26295568
  REMARK    GeneRIF: The authors propose that Lpd delivers Ena/VASP proteins to
            growing barbed ends and increases their actin polymerase activity
            by tethering them to actin filaments.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9699)
  AUTHORS   Chang,Y.C., Zhang,H., Brennan,M.L. and Wu,J.
  TITLE     Crystal structure of Lamellipodin implicates diverse functions in
            actin polymerization and Ras signaling
  JOURNAL   Protein Cell 4 (3), 211-219 (2013)
   PUBMED   23483482
  REMARK    GeneRIF: The crystal structure of Lpd cc-RA-PH presents a molecular
            model of Lpd enhances actin polymerization by oligomerization via
            two intermolecular contact sites.
REFERENCE   7  (bases 1 to 9699)
  AUTHORS   Eppert,K., Wunder,J.S., Aneliunas,V., Tsui,L.C., Scherer,S.W. and
            Andrulis,I.L.
  TITLE     Altered expression and deletion of RMO1 in osteosarcoma
  JOURNAL   Int J Cancer 114 (5), 738-746 (2005)
   PUBMED   15609301
  REMARK    GeneRIF: RMO1 is expressed and deleted in osteosarcoma
REFERENCE   8  (bases 1 to 9699)
  AUTHORS   Krause,M., Leslie,J.D., Stewart,M., Lafuente,E.M., Valderrama,F.,
            Jagannathan,R., Strasser,G.A., Rubinson,D.A., Liu,H., Way,M.,
            Yaffe,M.B., Boussiotis,V.A. and Gertler,F.B.
  TITLE     Lamellipodin, an Ena/VASP ligand, is implicated in the regulation
            of lamellipodial dynamics
  JOURNAL   Dev Cell 7 (4), 571-583 (2004)
   PUBMED   15469845
REFERENCE   9  (bases 1 to 9699)
  AUTHORS   Salazar,M.A., Kwiatkowski,A.V., Pellegrini,L., Cestra,G.,
            Butler,M.H., Rossman,K.L., Serna,D.M., Sondek,J., Gertler,F.B. and
            De Camilli,P.
  TITLE     Tuba, a novel protein containing bin/amphiphysin/Rvs and Dbl
            homology domains, links dynamin to regulation of the actin
            cytoskeleton
  JOURNAL   J Biol Chem 278 (49), 49031-49043 (2003)
   PUBMED   14506234
REFERENCE   10 (bases 1 to 9699)
  AUTHORS   Hadano,S., Hand,C.K., Osuga,H., Yanagisawa,Y., Otomo,A.,
            Devon,R.S., Miyamoto,N., Showguchi-Miyata,J., Okada,Y.,
            Singaraja,R., Figlewicz,D.A., Kwiatkowski,T., Hosler,B.A.,
            Sagie,T., Skaug,J., Nasir,J., Brown,R.H. Jr., Scherer,S.W.,
            Rouleau,G.A., Hayden,M.R. and Ikeda,J.E.
  TITLE     A gene encoding a putative GTPase regulator is mutated in familial
            amyotrophic lateral sclerosis 2
  JOURNAL   Nat Genet 29 (2), 166-173 (2001)
   PUBMED   11586298
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AI217860.1, AY494951.1,
            AJ584699.1, AC018891.9 and CA503229.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_213589.2.
            
            Summary: This gene encodes a protein that belongs to the
            Mig10/Rap1-interacting adaptor molecule/Lamellipodin family of
            adapter proteins, which function in cell migration. Members of this
            family contain pleckstrin-homology domains, Ras-association
            domains, and proline-rich C-termini. The protein encoded by this
            gene regulates actin dynamics through interaction with
            Ena/Vasodilator proteins as well as direct binding to filamentous
            actin to regulate actin network assembly. Alternative splicing
            results in multiple transcript variants. [provided by RefSeq, Jul
            2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ584699.1, AY494951.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000319170.10/ ENSP00000316543.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-43                AI217860.1         35-77
            44-1303             AY494951.1         74-1333
            1304-3010           AJ584699.1         1332-3038
            3011-9340           AC018891.9         44054-50383         c
            9341-9699           CA503229.1         17-375              c
FEATURES             Location/Qualifiers
     source          1..9699
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q33.2"
     gene            1..9699
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
                     /note="Ras association (RalGDS/AF-6) and pleckstrin
                     homology domains 1"
                     /db_xref="GeneID:65059"
                     /db_xref="HGNC:HGNC:14436"
                     /db_xref="MIM:609035"
     exon            1..191
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
                     /inference="alignment:Splign:2.1.0"
     CDS             192..3944
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     amyotrophic lateral sclerosis 2 (juvenile) chromosome
                     region, candidate 18; amyotrophic lateral sclerosis 2
                     (juvenile) chromosome region, candidate 9; ras-associated
                     and pleckstrin homology domains-containing protein 1;
                     lamellipodin; proline-rich EVH1 ligand 2"
                     /codon_start=1
                     /product="ras-associated and pleckstrin homology
                     domains-containing protein 1 isoform 1"
                     /protein_id="NP_998754.1"
                     /db_xref="CCDS:CCDS2359.1"
                     /db_xref="GeneID:65059"
                     /db_xref="HGNC:HGNC:14436"
                     /db_xref="MIM:609035"
                     /translation="MEQLSDEEIDHGAEEDSDKEDQDLDKMFGAWLGELDKLTQSLDS
                     DKPMEPVKRSPLRQETNMANFSYRFSIYNLNEALNQGETVDLDALMADLCSIEQELSS
                     IGSGNSKRQITETKATQKLPVSRHTLKHGTLKGLSSSSNRIAKPSHASYSLDDVTAQL
                     EQASLSMDEAAQQSVLEDTKPLVTNQHRRTASAGTVSDAEVHSISNSSHSSITSAASS
                     MDSLDIDKVTRPQELDLTHQGQPITEEEQAAKLKAEKIRVALEKIKEAQVKKLVIRVH
                     MSDDSSKTMMVDERQTVRQVLDNLMDKSHCGYSLDWSLVETVSELQMERIFEDHENLV
                     ENLLNWTRDSQNKLIFMERIEKYALFKNPQNYLLGKKETAEMADRNKEVLLEECFCGS
                     SVTVPEIEGVLWLKDDGKKSWKKRYFLLRASGIYYVPKGKAKVSRDLVCFLQLDHVNV
                     YYGQDYRNKYKAPTDYCLVLKHPQIQKKSQYIKYLCCDDVRTLHQWVNGIRIAKYGKQ
                     LYMNYQEALKRTESAYDWTSLSSSSIKSGSSSSSIPESQSNHSNQSDSGVSDTQPAGH
                     VRSQSIVSSVFSEAWKRGTQLEESSKARMESMNRPYTSLVPPLSPQPKIVTPYTASQP
                     SPPLPPPPPPPPPPPPPPPPPPPPLPSQSAPSAGSAAPMFVKYSTITRLQNASQHSGA
                     LFKPPTPPVMQSQSVKPQILVPPNGVVPPPPPPPPPPTPGSAMAQLKPAPCAPSLPQF
                     SAPPPPLKIHQVQHITQVAPPTPPPPPPIPAPLPPQAPPKPLVTIPAPTSTKTVAPVV
                     TQAAPPTPTPPVPPAKKQPAFPASYIPPSPPTPPVPVPPPTLPKQQSFCAKPPPSPLS
                     PVPSVVKQIASQFPPPPTPPAMESQPLKPVPANVAPQSPPAVKAKPKWQPSSIPVPSP
                     DFPPPPPESSLVFPPPPPSPVPAPPPPPPPTASPTPDKSGSPGKKTSKTSSPGGKKPP
                     PTPQRNSSIKSSSGAEHPEPKRPSVDSLVSKFTPPAESGSPSKETLPPPAAPPKPGKL
                     NLSGVNLPGVLQQGCVSAKAPVLSGRGKDSVVEFPSPPSDSDFPPPPPETELPLPPIE
                     IPAVFSGNTSPKVAVVNPQPQQWSKMSVKKAPPPTRPKRNDSTRLTQAEISEQPTMAT
                     VVPQVPTSPKSSLSVQPGFLADLNRTLQRKSITRHGSLSSRMSRAEPTATMDDMALPP
                     PPPELLSDQQKAGYGGSHISGYATLRRGPPPAPPKRDQNTKLSRDW"
     exon            192..311
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
                     /inference="alignment:Splign:2.1.0"
     exon            312..417
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
                     /inference="alignment:Splign:2.1.0"
     exon            418..923
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
                     /inference="alignment:Splign:2.1.0"
     exon            924..1001
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1002..1161
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1283
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1349
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1350..1493
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1494..1604
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1605..1703
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1824
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1825..1967
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
                     /inference="alignment:Splign:2.1.0"
     exon            1968..9699
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9537..9542
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
                     /note="hexamer: AATAAA"
     polyA_site      9566
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
                     /note="major polyA site"
     regulatory      9676..9681
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
                     /note="hexamer: AATAAA"
     polyA_site      9699
                     /gene="RAPH1"
                     /gene_synonym="ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2;
                     RalGDS/AF-6; RMO1"
ORIGIN      
        1 ccagtcagtc agtcagtcag tcagtcagtc agtcagtcag tcactgagcg cgcggcgcgg
       61 gagctgctgg cagtcgctgc gtctctggcg agggagcgcc gcgcctgggg aggaggcgga
      121 ggcagcggct ggaggagcgc gagcggcggt ttccttgccc ggggccgcgg gaaggccgac
      181 cgactgccgc gatggagcag ctatcagatg aagaaattga tcatggtgct gaagaagaca
      241 gtgacaagga agatcaggac ctggacaaaa tgtttggagc ctggcttgga gaactagaca
      301 aactcactca gagtttggat tctgacaagc ccatggaacc agtaaaaaga tctcctcttc
      361 gccaggaaac aaacatggcc aacttttctt accgcttctc catatacaac ttgaatgaag
      421 ctctgaatca gggagagact gtggatctgg atgccttgat ggctgatctt tgctctatag
      481 agcaggagct cagcagcatt ggttcaggaa acagtaagcg tcaaatcaca gaaacgaaag
      541 ctactcagaa attgcctgtt agccgacata cattgaaaca tggcaccttg aaaggattat
      601 cttcttcatc taataggata gctaaacctt cccatgccag ctactccttg gacgacgtca
      661 ctgcacagtt agaacaggcc tctttgagta tggatgaggc tgctcagcaa tctgtactag
      721 aagatactaa acccttagta actaatcagc acagaagaac cgcgtcagca ggcacagtga
      781 gtgatgctga agtacactct attagtaatt cctcccattc cagcatcact tccgcagcct
      841 ccagcatgga ctctttggat attgataaag taacacgccc tcaagagctg gatttgacac
      901 atcaagggca gccaattact gaggaagaac aggcagcaaa attgaaagct gagaagatca
      961 gagttgccct agagaaaatt aaagaggcac aagtgaaaaa gctggtgatc agagtccaca
     1021 tgtctgatga cagttctaaa acaatgatgg tggatgagag gcagacagta agacaagtac
     1081 tggataacct gatggacaaa tcccactgcg gttatagttt agactggtca ctggtagaaa
     1141 ccgtttctga attacaaatg gagagaatct ttgaagacca tgaaaacttg gttgaaaatc
     1201 ttcttaattg gacaagagat agccaaaaca agcttatatt tatggagcgt atagaaaaat
     1261 atgcactttt caaaaaccca cagaattatc ttttggggaa aaaagaaaca gctgagatgg
     1321 cagatagaaa caaagaagtc ctcttggagg aatgtttttg tggaagttct gtaactgtac
     1381 cagaaattga aggagtcctt tggttgaagg atgatggcaa gaagtcctgg aaaaagcgtt
     1441 attttctctt gcgagcatct ggtatctact atgttcccaa aggaaaagca aaggtctctc
     1501 gggatctggt gtgctttctc cagctggatc atgtcaacgt ttattatggc caggactatc
     1561 ggaacaaata caaagcacct acagactatt gtctggtgct gaagcatcca caaatccaga
     1621 agaaatctca atatatcaaa tacctttgtt gtgatgatgt gaggacactg catcagtggg
     1681 tcaatgggat ccgcattgca aagtatggga agcagctcta tatgaactac caagaagcct
     1741 tgaagaggac agagtcagcc tatgattgga cttccttatc cagctccagc attaaatcgg
     1801 gatccagttc ttccagcatc ccagagtctc agtcaaacca ctccaatcag tctgatagcg
     1861 gagtttctga cacccagcca gcaggacacg tccgttccca gagcattgtg agctccgtat
     1921 tctctgaagc ctggaaacga ggcactcagt tggaagagtc cagcaaggcc agaatggagt
     1981 ctatgaatcg gccctacact tcacttgtgc cccctttatc cccgcaacct aagatagtca
     2041 ccccctacac tgcttcacag ccttcaccac ctctacctcc tccgccaccc ccacctcctc
     2101 ctccaccacc ccctccacca ccccctcctc ccccactccc cagccagtct gcaccttctg
     2161 caggctcagc agccccaatg ttcgtcaagt acagcacaat aacacggcta cagaatgcgt
     2221 ctcagcattc aggggccctg tttaagccgc caacaccccc agtgatgcag tcacagtcag
     2281 tgaagcctca gatcctggta ccccccaatg gagttgttcc accaccccct ccccctcctc
     2341 cacccccaac cccaggctct gccatggccc agctaaagcc tgcaccgtgt gccccatccc
     2401 ttccacagtt cagtgccccg cctcctccac tgaagatcca tcaagttcag catattactc
     2461 aggtggctcc cccaacaccc cccccacctc ctcctatccc tgcacccctc cctccccaag
     2521 ctcccccaaa accccttgtg accatccccg caccaaccag caccaagact gtggcacctg
     2581 ttgtgactca agctgcacca cccacaccta ctcctccagt gcccccagca aaaaagcagc
     2641 cagctttccc tgcttcttac attccaccct ctccccctac ccctcctgtt ccagtacccc
     2701 cgccaacatt acccaagcaa cagagcttct gtgcaaaacc ccctccctct ccactgtcac
     2761 cggtgccctc ggtcgtgaag cagatagcca gccagtttcc accccctcca actccccctg
     2821 ccatggaatc tcagccctta aagcctgtcc cagcaaatgt agctccacag tcccctcctg
     2881 cagtaaaagc aaagcccaag tggcagccca gctccatccc agtcccttct ccggacttcc
     2941 ctcctccccc tcctgaaagc agcctggtgt ttcctcctcc acccccatca cctgtcccag
     3001 ccccaccacc accacctcca cccacagctt ctcctacccc tgacaaaagt ggatctccag
     3061 gcaaaaagac cagtaagacg tccagccctg ggggaaagaa accaccccca accccacagc
     3121 gcaactccag cattaaatcc agcagtggtg cagagcaccc cgagcccaag agaccctcgg
     3181 tggacagtct agtcagcaag tttacaccgc cagcagaatc agggtctccc agcaaggaga
     3241 ccctaccacc tcctgcagca ccccccaagc ctggaaaact caatctttct ggagtcaacc
     3301 ttcctggagt tctccaacaa gggtgtgtgt cagcaaaagc ccctgttctg agtgggcgtg
     3361 gaaaggactc cgtggtggaa tttccttctc ctccatccga ttctgatttt ccaccccctc
     3421 cacctgaaac agagcttcct ctgcccccca ttgagattcc agcagttttc tcgggaaaca
     3481 cctctccaaa agtggcagtc gttaatcctc aaccacaaca atggtctaaa atgtcagtga
     3541 agaaggcccc tccacccaca cgacccaaac ggaatgatag cacccgcctc actcaagctg
     3601 agatttctga gcagccaaca atggccacag ttgtgccaca agtgcccacc tctcccaaat
     3661 ccagccttag tgtccagcct ggattcctgg ctgacctcaa caggacactg caacgaaagt
     3721 ccatcactcg gcacggctca ctctcctccc gcatgtccag agcagaacca acagccacca
     3781 tggatgatat ggcattgcct ccaccacccc ctgaactgct gtctgatcaa cagaaggctg
     3841 gttacggagg cagtcatata tcaggctatg caacgttgcg gagaggaccc cctcctgctc
     3901 cccccaaaag agaccagaac accaagctct ccagagactg gtagccacca taggacttta
     3961 ttttcatgat atctgtaatc actgctacaa tcagctcacc tgatcatctg tgaattcagg
     4021 tgttcagagc ctcctggtat gatgttattc aggtagtgtc cagctatatg tgtatgtgtg
     4081 tgtgtacacg tgcatgtaca cacagctgta cagtgtgtgt atatatgtat acatatatgt
     4141 atgtgtatgt gtatatagag agagagctga gagttattct atttattcct tttctctcct
     4201 aatctgaaaa tgggtgttct gtattttggg tggaagaggc atagaagggg atgtgtgttg
     4261 tctcttaaga tttctatatc atgtggattg gaccaaaaac ttctaatcac ttatttagaa
     4321 ggtatttata agtgtctgtc catgtgtagc ctattcgtgc atgttgtgta ttatataact
     4381 aaggaataga tgtagaatgt gctatttctg gttgagaaaa atcaccagaa tgtttggtgt
     4441 atctataagg cttttgtgtt tgtttttccc cagttggctg aagttagaat tgcttgactg
     4501 acacttcatt gctatacatg aaggggcact ttaaatcagg aaaatctctc agcttcatag
     4561 aacgggtaac tagtgcagga tggggaaatg ttcacagaca tcatctgtat gtggttgtgc
     4621 atagaaagta aatacatggc gtaattaact cagctgttct agctgcagta ctgctgcagt
     4681 gatccaccca catttaggat gtgctgacag ataagctctt tgcctacaat acatggataa
     4741 ttagtgctat aattctggat agttcctttt tagtactgtt ttatgaagct ttatcaactt
     4801 ggcttcatga tcctcacttt gattgatttt aagaggatgg ataacacagt tatctctgta
     4861 atgttctgtc ccagtatgtc tttgggtcac cagttacctt cttaaaatat gtgctttagg
     4921 taggtgttat tacatatctg tagacaattg gtatatgaaa tatacacatc ctgtgcccca
     4981 atatggtgca ttatgaaaaa caaaatcatt ttctaaaatg cattttttga gcattgctct
     5041 atagaaggga agggtgatga gagaacagaa ctggcccctg tacaggtgtc attaatctgg
     5101 ttgtatatgg gttataatat gtaatacaaa aagctcatta agtatgggac tacatggaga
     5161 gggaagacag tttcatttat agctactggg gctaccagga cccttgctga ctgcagcctg
     5221 gttgtgatta gttcaggtta ctaggtgttc tgatggagtg ggacagtcca agtccagtaa
     5281 ctgacattac gttttatgcg tgtgcagttt ggtataacgt ggagtcagtg ctctaacgac
     5341 acactatact tctatatgct tttttctgtg aattttcctt ggtacatgag agaaataagt
     5401 actctcatca acttatgata aattggacta ttaggaataa aacaatctca gagcagctcc
     5461 taaacaagag aataaaaatg ggccatccca gcacttataa ggggagcaca tcttgtaatg
     5521 aaagtctgtg cctattgtta atgattacca atgcaaactg cagaaatcag ttgacattgt
     5581 cccacaagga aaaagtgatg ccagtaaagt gggggaagag atggtaaata attctagaat
     5641 gagcaaaagt gcttaattag tacagtatac tcaaggtctt gcataaccca cactccacga
     5701 aaaccgcatc gtaaacagag agtgaacagc acctctccat tgctacatct ccagaatatt
     5761 gttcggttat gttcatgtgg ttatggagca ataagaatat ataccattaa tttttaggtc
     5821 tttcaaattg ttcaccaagt tttgtgtttg atatttatga agtgatcttt aacacatctg
     5881 cattgttaat ttaagcatgc acagaaattt gggtaatgtt tgagtagatt gccaagcgct
     5941 actgtgattc atgaatgaat ttctcatagt cttctgcctg cagttttata gtttgagggg
     6001 cacccatatt tgtgggttag agttagtaag aatatgaatc cagttatcaa agtattcatc
     6061 cctgaggttt tgagttttat tgtggtggtg attttctcat gagaaggtag acaacacttc
     6121 agtttttctt gagggggaaa aaatgtaccc gctaaccttt tttcacccat gatttgagcc
     6181 ctaaaacaac agcctttatg ttttcagtgc aggatttctt ggaatttctt gcttgtatca
     6241 atcctgatga tagccatcct gaacagatgc ttctggtctg ttttcctgga atagcagaca
     6301 tccttttgaa cactaatgat gccaggaaga gagtaaggct agaggaggag actcatttta
     6361 accccagtcc agtggcttgt ccgacaacag gccatttccc tttacattcc tccacagaca
     6421 gaaatggcta ttaaaaatgt tttaacctca tgtatgaact gaatttaaaa aaccactaaa
     6481 gtgatacttt atacttctgt tttactccct agtgggtttt ctttaatttg gatttttgtt
     6541 gggatgtagc ttttcctaga ctgtatttct gtagctcttt ttctctggtg tattcatgga
     6601 aagcatctac tgccgcacga cactagtact gtgccttctt tcactgccct ctgctctgca
     6661 gctctcaccc agcttggctg cttcaagtct gtgcagttga cttggctgta ctgaaaagtg
     6721 aacagcatgg attaactcat cctgctgctg agggcagtgc aaacaggtgc ttattagagg
     6781 tagtgacttg cttaattagt gacactccta atttctacta tggataattt caaagtagaa
     6841 tcactctatg cttagagttt tggcaccaat gctgtagggc agcagaatct attctcagta
     6901 aaataactta tggtttatta gatattctgt attggatttt accagcttga cttttactgc
     6961 tcagatgctt tctttccccc ccttagacgc tgtaattctc ttgggaagag taactattct
     7021 taaggttttt acagataccc accttagttg taaattggat agtttatatt tctgggactt
     7081 tttaaatgaa aatgtggaat gttaagttac aaaagacttt tcatcagaaa atttcaaaca
     7141 aagtaaacat ggcgttttat agtcctctaa aatctaggtg ctcccaccca ccaaaggcat
     7201 atcctgcaaa gggctgtgaa ctatcttggt gaactgtctt gggtcccctt tccatgtatg
     7261 ttttcttgtc actgaaaaca aacaacgctg agtttatcaa gaaaatttaa attgggggat
     7321 cataataatt ccaaccaagt gacaactctg acatcaaggt tattaggagc tgtacatcca
     7381 attcaagttt tatttgctgc tattctggga gaataaactt gtatatggag aataaactaa
     7441 taaacttgta tcgaggaaat ccataaagtt ataaattagc ctgaaaaata tttcaggtaa
     7501 tggtgtggat tggcctgctt tgactctcag ccaccaacag aaatctttgt cacctttgtt
     7561 cctcagctaa aagtaatttt gttataaaca caaagtgact ttaaacaggt aaaaaaccca
     7621 ttcctatttt tgtacattac caaaagtttt tcatatacct acagagctaa ctaattacaa
     7681 ctgatttaat ccactcaagt ttagaccagt taaacccata ggatcctgta tggttatcaa
     7741 tgtgatgcct tgcttttcat aaaataggta taattgggtc atacacttga cgagagggtg
     7801 actgtttcta ggggaagaaa accctttaga ttgcaggtaa ctttcacttt ttttttttta
     7861 atatacactt tacatttgta taaattatgc agggtactcc taaccctgta gaaatgtatg
     7921 acctctcaca aagttgagat ttgatccaaa gagaaatgca agtataaaag aattagatac
     7981 ttattatctt ttaaggtttt tttttttttt tggtagagat gggggtctca ctgtgttgcc
     8041 caggctggtc tcaaactcct ggccacaagt gatcttcctg cctcagcctc ccaaaatgct
     8101 gagattacag gcatgagcca ctgtacccag cctttcctta taaaattcaa agagaaaatt
     8161 tctacacctt tatccctcaa ataaaacaag tgctcagttc ttaccgtgcc cttgcaaggt
     8221 ctatatgtaa aagaaatctg aaatttagct gtagaataaa acttgataaa taaaaagaaa
     8281 aaacatacat ttctccagtt ggtttgctct ttgcttgttg aagtaataaa ccgttttaaa
     8341 gagaaaatac ttgctgtaaa cccccagtgc cttcaactct tttggcagaa tatttttaaa
     8401 gaaatccagc aagcaaactt tgaggtgcta atgaaagtaa aggaaggtgg tatttctagt
     8461 tttggcagaa atgaaaagtg tctcacaaga gacatcacta cccacgtggg gtctggctgc
     8521 tttctaccaa agacatttag agaagtgaat tgagtcaggg tgatggtgaa cactacatat
     8581 tttatagatg gttaagttga gaattaatta tgtttatcat ggatggctac taataccaag
     8641 ctcatgattg ttgcagcctc aacgtcttag gcagtaaaac ttgtctgcag cactaaaggg
     8701 ggagaaaccc ttatattttg caaactgtcc attcgttaaa tttattgtaa cctaatacca
     8761 aaaactgccg tttttcatat tatttcccca cctcctactt tttttttttt ttttgctact
     8821 tgtaaaataa ccccttctag aaaataagca ttaactggaa tgtttcaaac aattttgctt
     8881 cattttacta tcagccacta gtgaactctt acagagatgt acatttaaga taaaattagc
     8941 ttgtgctaag tgttttaaaa acattgttta ctgttaaagg ggaattgcac attatattta
     9001 actgggattg ctccctccct cagttcttta aaaaacaaga gtcaaggctc acaccaactt
     9061 gtaggctgtg ggagctttgc cataggtaga tacaatgtag aagtatactt ttttaaagca
     9121 tgaagaagac aaggaacttc attataatgt accaggtaga ggacattatt attcaaagga
     9181 ttatgcacag ctcagtgaag atgaagttac aatttttctc gcagctttgt tgctattatt
     9241 ttcttctgca taaatgtatg ctcatttcat tatgtgcctt gctccctgat tgtgcaaagc
     9301 tatatatata tatatatata tatagataga tagatagata tatgagagag atatattcag
     9361 tactactgag gatgtttttc tgaggatgtt tttgttctgc tggattaagt tattttccaa
     9421 gttactcttg ccagttatgt cagtaaacta ttgtaatggc ttagcacact agtcgtacag
     9481 tcagtgtaaa tgtttttcat ttacatgttt tcattatatc agcttatcaa atccttaata
     9541 aaaaaaattc atagatttca tttaaacatg gggactttga gtgtttgtgg atcggcacca
     9601 ttaagaatct ggttttgcat ttcatgtcag ggtacccaaa acataggcca tacacagaag
     9661 aaattaagtg tggtaaataa actagtttga tatgttcaa
//
