LOCUS       NM_016340               8350 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens Rap guanine nucleotide exchange factor 6 (RAPGEF6),
            transcript variant 2, mRNA.
ACCESSION   NM_016340
VERSION     NM_016340.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   2  (bases 1 to 8350)
  AUTHORS   Consonni,S.V., Brouwer,P.M., van Slobbe,E.S. and Bos,J.L.
  TITLE     The PDZ domain of the guanine nucleotide exchange factor PDZGEF
            directs binding to phosphatidic acid during brush border formation
  JOURNAL   PLoS One 9 (5), e98253 (2014)
   PUBMED   24858808
  REMARK    GeneRIF: local accumulation of PDZGEF at the apical membrane during
            establishment of epithelial polarity is mediated by electrostatic
            interactions between positively charged side chains in the PDZ
            domain and negatively charged phosphatidic acid.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8350)
  AUTHORS   Hauri,S., Wepf,A., van Drogen,A., Varjosalo,M., Tapon,N.,
            Aebersold,R. and Gstaiger,M.
  TITLE     Interaction proteome of human Hippo signaling: modular control of
            the co-activator YAP1
  JOURNAL   Mol Syst Biol 9, 713 (2013)
   PUBMED   24366813
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8350)
  AUTHORS   Monteiro,A.C., Sumagin,R., Rankin,C.R., Leoni,G., Mina,M.J.,
            Reiter,D.M., Stehle,T., Dermody,T.S., Schaefer,S.A., Hall,R.A.,
            Nusrat,A. and Parkos,C.A.
  TITLE     JAM-A associates with ZO-2, afadin, and PDZ-GEF1 to activate Rap2c
            and regulate epithelial barrier function
  JOURNAL   Mol Biol Cell 24 (18), 2849-2860 (2013)
   PUBMED   23885123
REFERENCE   5  (bases 1 to 8350)
  AUTHORS   Ulbricht,A., Eppler,F.J., Tapia,V.E., van der Ven,P.F., Hampe,N.,
            Hersch,N., Vakeel,P., Stadel,D., Haas,A., Saftig,P., Behrends,C.,
            Furst,D.O., Volkmer,R., Hoffmann,B., Kolanus,W. and Hohfeld,J.
  TITLE     Cellular mechanotransduction relies on tension-induced and
            chaperone-assisted autophagy
  JOURNAL   Curr Biol 23 (5), 430-435 (2013)
   PUBMED   23434281
REFERENCE   6  (bases 1 to 8350)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
REFERENCE   7  (bases 1 to 8350)
  AUTHORS   Fuentes,E.J., Der,C.J. and Lee,A.L.
  TITLE     Ligand-dependent dynamics and intramolecular signaling in a PDZ
            domain
  JOURNAL   J Mol Biol 335 (4), 1105-1115 (2004)
   PUBMED   14698303
REFERENCE   8  (bases 1 to 8350)
  AUTHORS   Kuiperij,H.B., de Rooij,J., Rehmann,H., van Triest,M.,
            Wittinghofer,A., Bos,J.L. and Zwartkruis,F.J.
  TITLE     Characterisation of PDZ-GEFs, a family of guanine nucleotide
            exchange factors specific for Rap1 and Rap2
  JOURNAL   Biochim Biophys Acta 1593 (2-3), 141-149 (2003)
   PUBMED   12581858
REFERENCE   9  (bases 1 to 8350)
  AUTHORS   Kozlov,G., Banville,D., Gehring,K. and Ekiel,I.
  TITLE     Solution structure of the PDZ2 domain from cytosolic human
            phosphatase hPTP1E complexed with a peptide reveals contribution of
            the beta2-beta3 loop to PDZ domain-ligand interactions
  JOURNAL   J Mol Biol 320 (4), 813-820 (2002)
   PUBMED   12095257
  REMARK    GeneRIF: the second PDZ domain from human phosphatase hPTP1E in
            complex with a C-terminal peptide from the guanine nucleotide
            exchange factor RA-GEF-2 has been determined
REFERENCE   10 (bases 1 to 8350)
  AUTHORS   Gao,X., Satoh,T., Liao,Y., Song,C., Hu,C.D., Kariya Ki,K. and
            Kataoka,T.
  TITLE     Identification and characterization of RA-GEF-2, a Rap guanine
            nucleotide exchange factor that serves as a downstream target of
            M-Ras
  JOURNAL   J Biol Chem 276 (45), 42219-42225 (2001)
   PUBMED   11524421
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF478567.1, BC144627.1, DA817493.1 and AL832899.1.
            
            On Jun 2, 2019 this sequence version replaced NM_016340.5.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon compared to variant 1. The resulting protein (isoform 2) is
            shorter than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF478567.1, SRR14038197.1753337.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000509018.6/ ENSP00000421684.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4553              AF478567.1         8-4560
            4554-5712           BC144627.1         4460-5618
            5713-6233           AF478567.1         5720-6240
            6234-6640           DA817493.1         107-513
            6641-8350           AL832899.1         2520-4229
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q31.1"
     gene            1..8350
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /note="Rap guanine nucleotide exchange factor 6"
                     /db_xref="GeneID:51735"
                     /db_xref="HGNC:HGNC:20655"
                     /db_xref="MIM:610499"
     exon            1..268
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     CDS             200..5005
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /note="isoform 2 is encoded by transcript variant 2; PDZ
                     domain-containing guanine nucleotide exchange factor I;
                     PDZ domain containing guanine nucleotide exchange factor
                     (GEF) 2; Rap guanine nucleotide exchange factor (GEF) 6;
                     PDZ domain-containing guanine nucleotide exchange factor
                     2"
                     /codon_start=1
                     /product="rap guanine nucleotide exchange factor 6 isoform
                     2"
                     /protein_id="NP_057424.3"
                     /db_xref="CCDS:CCDS34225.1"
                     /db_xref="GeneID:51735"
                     /db_xref="HGNC:HGNC:20655"
                     /db_xref="MIM:610499"
                     /translation="MNSPVDPGARQALRKKPPERTPEDLNTIYSYLHGMEILSNLREH
                     QLRLMSARARYERYSGNQVLFCSETIARCWYILLSGSVLVKGSMVLPPCSFGKQFGGK
                     RGCDCLVLEPSEMIVVENAKDNEDSILQREIPARQSRRRFRKINYKGERQTITDDVEV
                     NSYLSLPADLTKMHLTENPHPQVTHVSSSQSGCSIASDSGSSSLSDIYQATESEVGDV
                     DLTRLPEGPVDSEDDEEEDEEIDRTDPLQGRDLVRECLEKEPADKTDDDIEQLLEFMH
                     QLPAFANMTMSVRRELCSVMIFEVVEQAGAIILEDGQELDSWYVILNGTVEISHPDGK
                     VENLFMGNSFGITPTLDKQYMHGIVRTKVDDCQFVCIAQQDYWRILNHVEKNTHKVEE
                     EGEIVMVHEHRELDRSGTRKGHIVIKATPERLIMHLIEEHSIVDPTYIEDFLLTYRTF
                     LESPLDVGIKLLEWFKIDSLRDKVTRIVLLWVNNHFNDFEGDPAMTRFLEEFEKNLED
                     TKMNGHLRLLNIACAAKAKWRQVVLQKASRESPLQFSLNGGSEKGFGIFVEGVEPGSK
                     AADSGLKRGDQIMEVNGQNFENITFMKAVEILRNNTHLALTVKTNIFVFKELLFRTEQ
                     EKSGVPHIPKIAEKKSNRHSIQHVPGDIEQTSQEKGSKKVKANTVSGGRNKIRKILDK
                     TRFSILPPKLFSDGGLSQSQDDSIVGTRHCRHSLAIMPIPGTLSSSSPDLLQPTTSML
                     DFSNPSDIPDQVIRVFKVDQQSCYIIISKDTTAKEVVFHAVHEFGLTGASDTYSLCEV
                     SVTPEGVIKQRRLPDQFSKLADRIQLNGRYYLKNNMETETLCSDEDAQELVKESQLSM
                     LQLSTIEVATQLSMRDFDLFRNIEPTEYIDDLFKLNSKTGNTHLKRFEDIVNQETFWV
                     ASEILTEANQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLASVARLRGTWEKL
                     PSKYEKHLQDLQDIFDPSRNMAKYRNILSSQSMQPPIIPLFPVVKKDMTFLHEGNDSK
                     VDGLVNFEKLRMISKEIRQVVRMTSANMDPAMMFRQRSLSQGSTNSNMLDVQGGAHKK
                     RARRSSLLNAKKLYEDAQMARKVKQYLSSLDVETDEEKFQMMSLQWEPAYGTLTKNLS
                     EKRSAKSSEMSPVPMRSAGQTTKAHLHQPHRVSQVLQVPAVNLHPIRKKGQTKDPALN
                     TSLPQKVLGTTEEISGKKHTEDTISVASSLHSSPPASPQGSPHKGYTLIPSAKSDNLS
                     DSSHSEISSRSSIVSNCSVDSMSAALQDERCSSQALAVPESTGALEKTEHASGIGDHS
                     QHGPGWTLLKPSLIKCLAVSSSVSNEEISQEHIIIEAADSGRGSWTSCSSSSHDNFQS
                     LPNPKSWDFLNSYRHTHLDDPIAEVEPTDSEPYSCSKSCSRTCGQCKGSLERKSWTSS
                     SSLSDTYEPNYGTVKQRVLESTPAESSEGLDPKDATDPVYKTVTSSTEKGLIVYCVTS
                     PKKDDRYREPPPTPPGYLGISLADLKEGPHTHLKPPDYSVAVQRSKMMHNSLSRLPPA
                     SLSSNLVACVPSKIVTQPQRHNLQPFHPKLGDVTDADSEADENEQVSAV"
     exon            269..339
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            340..396
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            397..480
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            481..550
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            551..694
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            695..826
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            827..1004
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1141
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1300
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1301..1453
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1618
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1619..1726
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1727..1930
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1931..2039
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2040..2280
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2281..2438
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2439..2679
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2680..3063
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3064..3275
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3276..3399
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3400..3620
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3621..3809
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3810..3944
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3945..4173
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            4174..4664
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            4665..4979
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     exon            4980..8350
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8332..8337
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /note="hexamer: AATAAA"
     polyA_site      8350
                     /gene="RAPGEF6"
                     /gene_synonym="KIA001LB; PDZ-GEF2; PDZGEF2; RA-GEF-2;
                     RAGEF2"
                     /note="major polyA site"
ORIGIN      
        1 agacttgtct ccggccagtc gcgcgtgggt agaggcctta ggcgggcgtt gcgaagggtt
       61 gtttgctaga gttcggacct tgttacggcg cgaagttccc tccttggggc gggggaaagg
      121 taccgcaccc gctacctagt gtgaatgaac tgtggcgtgg gctgctaagg gcaggctgcg
      181 gagtacccgg gccgtggcca tgaactcacc cgtggaccct ggcgctaggc aggcgttgag
      241 gaagaagcca cccgagcgga ctcccgagga cttaaatact atttattctt atcttcatgg
      301 aatggaaata ttatcaaatc tcagggaaca tcagcttaga ttaatgtctg caagagcacg
      361 ctatgagaga tacagtggca atcaggttct cttttgttca gaaacgattg ccagatgttg
      421 gtatatccta ctttctggat ctgtgcttgt gaaaggctcc atggtcttgc ctccttgcag
      481 ttttggtaag cagtttggag gaaaaagagg atgtgattgt cttgtattag agccttcaga
      541 aatgattgtg gtagagaatg ccaaagataa tgaagatagt attctacaaa gagaaattcc
      601 tgccagacaa tcccgaagaa gatttcggaa aattaactat aaaggagagc gccaaaccat
      661 tactgatgat gtggaggtta acagctatct ttctcttcca gctgatctta ccaagatgca
      721 tctcacagaa aaccctcatc cacaggtgac tcatgtgtct tctagtcagt ctggttgtag
      781 cattgccagt gactctggaa gcagcagttt atctgatatc tatcaggcta cggagagtga
      841 ggtaggagat gtagatttga cacgtcttcc agaaggacct gttgattctg aggatgacga
      901 agaggaagat gaagagattg atcgaacaga tccattgcag gggcgagatc ttgttcgaga
      961 atgtcttgaa aaagaacctg cagacaaaac tgatgatgac attgaacaat tgctggagtt
     1021 tatgcaccag ctccctgcat ttgcaaacat gaccatgtct gtaaggagag aactctgctc
     1081 agtgatgatt tttgaagtgg tagagcaggc tggagctatt attcttgaag atgggcaaga
     1141 gcttgactca tggtatgtta ttttaaacgg cactgtggaa atcagtcatc cagatggaaa
     1201 agttgaaaat ttgtttatgg gaaatagttt tggaattact cccactctgg ataagcagta
     1261 catgcatgga attgtcagga ctaaagtaga tgattgtcag tttgtctgca tagcccagca
     1321 agattattgg agaattttaa accatgtgga aaaaaatacc cataaagttg aggaagaggg
     1381 agaaattgtt atggtacatg agcatcggga actagaccgg agtggaacca ggaaaggaca
     1441 cattgtgatc aaggcaacac ctgagcgtct cataatgcat ttaatagaag aacattccat
     1501 cgtggatcca acttatatag aagattttct attaacttac aggacatttc ttgaaagtcc
     1561 tttggatgtt gggatcaaac tattggaatg gtttaagatc gacagcttaa gagataaggt
     1621 gacacggatt gtattattat gggtaaataa tcattttaat gattttgaag gtgaccctgc
     1681 tatgactcga tttctagagg aatttgaaaa aaatctggaa gatacaaaga tgaatggtca
     1741 tctccggtta ttgaatattg cctgtgctgc aaaggctaag tggagacagg ttgtgctgca
     1801 aaaggcttcc cgcgagtccc ctctacaatt cagccttaat ggagggagtg agaagggatt
     1861 tggtattttt gttgaaggag tagaacctgg tagcaaagct gctgattcag gactgaaacg
     1921 tggtgatcag attatggaag taaatggaca aaactttgag aatattacat ttatgaaagc
     1981 cgttgaaatt ttgaggaata atactcatct tgcacttact gtgaagacca acatttttgt
     2041 gttcaaagag ttacttttta ggactgaaca agagaaatct ggtgttcctc atattcccaa
     2101 aattgctgaa aaaaaaagta atcgccattc tatccagcat gtgccaggag atattgaaca
     2161 gacatcacag gagaaaggaa gtaagaaagt taaagcaaat actgtttcag gtggaagaaa
     2221 caaaatcagg aagattttgg ataaaacacg atttagtatc ttgcctccaa agctatttag
     2281 tgatggaggc ctaagccaat cacaagatga cagcattgtg ggaacaaggc actgtaggca
     2341 tagtctggct ataatgccca tccctggaac actctcatcc agcagccctg atctcctgca
     2401 gcctaccacc agtatgttgg atttttccaa tccttcagat atccctgatc aagttataag
     2461 agttttcaaa gtggatcagc aaagttgcta cattatcatc agtaaagaca ccacagctaa
     2521 agaagtagtt tttcatgctg ttcatgaatt tggtttgacc ggtgcatccg acacatattc
     2581 tctctgtgaa gtttctgtta ctcctgaggg tgtcataaaa cagagaagac ttccagatca
     2641 gttctccaaa ttagctgata gaattcaact caatggaagg tattacttaa aaaataacat
     2701 ggaaacagaa accttatgtt cagatgaaga tgctcaagaa ctagttaagg aaagccagct
     2761 atccatgctg cagctcagta ccattgaggt ggccacccag ctgtcaatga gggactttga
     2821 tttgtttcgt aatattgaac cgactgagta catcgatgac ctttttaagt taaattccaa
     2881 aacaggaaat actcatttga agaggtttga ggacattgta aaccaagaga cattctgggt
     2941 tgcctcagaa attttaactg aagcaaatca gctcaaacga atgaagatta ttaagcattt
     3001 tattaaaatt gcacttcatt gtcgagaatg taagaacttc aattccatgt ttgcaataat
     3061 aagtggcttg aacctggcat ctgtagcaag actcagagga acttgggaaa agttaccaag
     3121 caaatacgag aaacatcttc aagatctaca agacattttt gatccatcta gaaacatggc
     3181 aaagtataga aatattctta gtagtcaaag tatgcagcct ccaattattc cactcttccc
     3241 tgttgtcaag aaagatatga catttctaca tgaaggaaat gactccaaag tagatggttt
     3301 agtaaacttt gagaagttaa gaatgatttc caaggaaatc cgccaagttg ttcgaatgac
     3361 ttctgctaac atggacccag ctatgatgtt tcgacagagg tcactgagtc aaggaagcac
     3421 aaattcaaac atgctggatg ttcagggagg tgctcacaaa aaaagggcac gccgcagctc
     3481 tctgcttaat gccaagaagc tatatgagga tgcccaaatg gcaaggaagg tgaagcagta
     3541 tctttccagt ctcgatgtag agacagatga ggagaagttc cagatgatgt cattacagtg
     3601 ggagcctgca tatggtacct tgaccaagaa tttaagtgag aaaagatcag ccaaatcatc
     3661 tgaaatgtct ccagtgccta tgaggtcagc tggccaaaca actaaagccc acttgcatca
     3721 accccacaga gtaagccagg tgcttcaggt gccagctgtt aatttgcacc ccatcaggaa
     3781 gaagggacaa acaaaagacc ctgcactgaa tacaagttta cctcagaaag ttttaggaac
     3841 aactgaagaa ataagtggta agaagcatac agaagacact atttctgtgg cgtcatcttt
     3901 acattctagt cctcctgcat ctcctcaagg ctcccctcac aaaggttaca cacttattcc
     3961 atcagctaaa tctgacaact tgtctgactc cagccatagt gagatttctt cacggtccag
     4021 catcgtgagc aattgttctg ttgactccat gtctgcagct ctacaggatg aacggtgttc
     4081 ctctcaggcc ctggcagtcc ctgaatccac tggggcattg gaaaagacag agcacgcttc
     4141 agggatagga gatcatagtc aacatggccc tgggtggaca ctcttgaagc catctctaat
     4201 caagtgttta gctgtctcat cgtctgtgag caatgaagag atttctcaag agcatatcat
     4261 tatagaagca gctgacagtg gtcgtggaag ttggacttcg tgttcaagca gctcccatga
     4321 caacttccaa agccttccaa acccaaaaag ctgggatttt ttgaactctt acagacatac
     4381 ccatttggat gaccccattg ctgaagttga acccactgac tctgagccct attcctgttc
     4441 taaaagctgc tctagaactt gtgggcagtg taaaggaagc ctagagagaa agagttggac
     4501 ctcctccagt tctctgtctg acacgtatga accaaactat gggacagtta aacagagagt
     4561 attggagagc accccagctg agtcatctga aggcttggac cccaaggatg ccactgaccc
     4621 agtttataaa actgtcactt caagtacaga aaagggcttg attgtgtact gtgtcacctc
     4681 acccaagaag gacgataggt atagggagcc acctcccact cctccaggat atttggggat
     4741 ttctttagcg gacctaaagg aaggacccca cacacaccta aaacctccag attatagtgt
     4801 ggcagtgcag aggtcaaaga tgatgcataa cagcctctct agactgccac cagcttctct
     4861 cagtagcaac ctcgtggcct gtgttccatc gaagattgta actcagcctc agaggcataa
     4921 tttgcagcca ttccatccta aactaggaga tgtgactgat gcagatagcg aagcagatga
     4981 aaatgaacaa gtttcagcag tctagccttt ggatgaccta tttgaaaacc actgaaagtc
     5041 gtggaggaat gggcaagaac cacctcatga ttctgcaggc cattgctaac gaacagctca
     5101 ttgctacaac cagtccagag gttttattcc ctctactccg agcaatgaaa tagacctgag
     5161 ttatgcttcc tttcatttaa tttctgcaga taaatagttt cctgagcaat ggatgctatg
     5221 cctggatacc agtctccact ttgcacgccg gaactgcctt gggaccacag ttacagaaaa
     5281 aatgtaaact cagagtgatc cttgtgtata ttgctataga tttttcttta acaagctatt
     5341 ttaaagataa tggcattatt atttccaagc catagcttgg gctgaaggac aaattgaaat
     5401 tgtctgccaa taccaaggat attcttgtat atttgaaaaa taacttatta tttgaattgt
     5461 tgtggttttg tttgtatttg agagctcttg ttagctgata ttcatgtttg aggtcataaa
     5521 attgtctctg gtctgaccaa acagaagtca tctttacaga ggtgatatgc ttgatctaca
     5581 cagagatgtg acttgatctg tagcaccaat gcaatgtagg tctcagtttg agagaaatag
     5641 gaagcccttt gcagttgagg tgttaggaac ctgctggtca tggtgtggaa ggccaaatga
     5701 agctgccaca gggtttcttg tcagtccttt gggaaatggg agggagtagt ttggggagga
     5761 gggtgggaac cctaatttcc acagaatgaa attttgatgt taaatgacat gtatacaaat
     5821 tcttccttaa gtgaaagtta tgctgcatcg aattgtaact gaaagtatag atccaacaaa
     5881 tagagactgg gttctagaga gttctggtct atagaaaccc aaaactaaaa tctctcataa
     5941 ctcaagtatg gaatactttt tttaaagaaa ttcttatcat gggtgttgta ataatgaaga
     6001 cgaatttgac tttatgcagt gttctgcagc atgcctcccc cacatctcat agcaccaggt
     6061 tgtgtctgac ctgacatacc ctgcagctct cagctggctg cagtaacatt ttgtgggaga
     6121 aagaggagct ggagttacag aaatgattgt ctcttggttc tcagttttta gcccttgaga
     6181 ggacatactt ttccagcctc atgggtatgg cactcttaat taaaatttca gtgactgttt
     6241 actggatgag gcagattttt cacatttttg caaattaaat atattttata tatattaagt
     6301 ttaatttttt cagttttttt aatgtaaaag caagtgaaat tttaataaac ttctgtaatt
     6361 accaaaaaaa aaaaaaaaaa aaaagccaaa gccattaacg tgcactaccc agatcaactt
     6421 ggagccaaaa ggggtttact tctgaatggc tgtccgtatg tgtccacata ggatttagtt
     6481 ggtttgtggc ctcctttata gaaatgtgtt gtctttccct agctgtctct gctgtctgtc
     6541 ttcctgcata tcccccttca ttttaaaatc atgaatcagt tgtaagtggt aaatggtagt
     6601 agttcatctt tcccatcaac atgattgtct ctttatttta ggaataatag catttcaaaa
     6661 attcctgtgg taaagaaaca ttaatctgaa acttaagcct tagaaattaa tcgcaggtat
     6721 gatagaatga ggcttggtgg ttccatagct ttcttggtcc tttgaaatgg gaaataacct
     6781 gaaccaaaaa tcctagtttg aaagttacac ttcagtgatt tggcctataa acagtggtcc
     6841 tatgattata cattttttcc ttcactgatt tgcataattt tgttttcatg gccacagcaa
     6901 aacaatgtga aatatatttt tgctataccc acttttttta tcaaattcag atgtctactc
     6961 tcaccatttt atattcattt ccccagaaaa atgtgctgtt ttcataatgt ctttatttag
     7021 caggtctttc aaaccaaatg gaagccttac ctgtctttgt ccttaatagt gtaaatatct
     7081 tgcacagagt gttgaggcct atggttttgg acttgcacct tacagctgcc tttgttttat
     7141 ctatctagct ttaactgtga gtgttcagat cagctgatca cattgatcag gagccaaagc
     7201 caaatgcttt gtagtttgtt atgtatgtat cccttaaaaa atcatgaggt tttgattttt
     7261 atattttaac taagggtaca ttcctgcctt ctcttagttt gtcatatgac tagaacaaga
     7321 gaagtcttaa gctgcattta ctccaattcc agatctccat ttgtttttgt tttataaatt
     7381 tataatctta atgaagagac tagtgccctc tcttcctgcc acaactctca atctgccaag
     7441 actgttgaag cagttaacgc tcagtgcaaa cttttgccaa atagtaagtt tcaacaaaat
     7501 atctttatag tgaaagattt tgagaattag taaatccagt acatgattcc ataaaagctt
     7561 attaaagtta ataattctaa tttactccaa atgattactt tcaatatgat ctttggggag
     7621 aaaaagcctt tagtgaattt tcttgagggt gataaatcac atcattgcga tctatatcac
     7681 ctccttgaga atctctccca ggctgaaaat gtgatggtaa tcatgggtgt ttttaagaac
     7741 aaatctaatt tttatcaagt gttctttcct ctacaaaaaa ggaaggagga gaaaaagctt
     7801 ccttaaaact gattattttg attcaatttt aagtttctaa aaaaggtgat attaaagtat
     7861 atgtattcct tcagcattac cttaaaggct agaaaaacgg gataaatctt cagaaaagtg
     7921 tcaaaagtgt cacagtacat gttcaaattt aaacttgaat cctggttcat ctttgacttt
     7981 tgttgtgcat ttttaatgct tctttatttt tgatacacag aatgcagttg aacttgatct
     8041 tttaaaattg agacatgtaa gctaatcagc aaaagacaga aacaaaactg cacatctgta
     8101 ataatcagat agcaattcta ctgcacaatt cattgattgt aaggtatcct gtaacaagca
     8161 atgtttgtct cctatttttt gatacctctt aaacttatgt cttttagaaa gacagtgcct
     8221 ctgtatgctt tttgtatttt tactgagtgt aaatatttgt tatatttttg gttaagagaa
     8281 tgtaaaaata ccatttatta ttatcatatc tactaataca ttggtggaat caataaagaa
     8341 tctacattaa
//
