LOCUS       NM_012294               6916 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens Rap guanine nucleotide exchange factor 5 (RAPGEF5),
            transcript variant 1, mRNA.
ACCESSION   NM_012294 XM_005249914 XM_928602 XM_935655 XM_937844
VERSION     NM_012294.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Cheng,D., Chu,F., Liang,F., Zhang,N., Wang,J. and Yue,W.
  TITLE     Downregulation of circ-RAPGEF5 inhibits colorectal cancer
            progression by reducing the expression of polypeptide
            N-acetylgalactosaminyltransferase 3 (GALNT3)
  JOURNAL   Environ Toxicol 39 (8), 4249-4260 (2024)
   PUBMED   38775215
  REMARK    GeneRIF: Downregulation of circ-RAPGEF5 inhibits colorectal cancer
            progression by reducing the expression of polypeptide
            N-acetylgalactosaminyltransferase 3 (GALNT3).
REFERENCE   2  (bases 1 to 6916)
  AUTHORS   Wang,C. and Yang,X.
  TITLE     CircRAPGEF5 sponges miR-582-3p and targets KIF3A to regulate
            bladder cancer cell proliferation, migration and invasion
  JOURNAL   Int Immunopharmacol 131, 111613 (2024)
   PUBMED   38489970
  REMARK    GeneRIF: CircRAPGEF5 sponges miR-582-3p and targets KIF3A to
            regulate bladder cancer cell proliferation, migration and invasion.
REFERENCE   3  (bases 1 to 6916)
  AUTHORS   Zheng,J., Wang,Y., Tao,L., Cai,J., Shen,Z., Liu,Y., Pan,H., Li,S.,
            Ruan,Y., Chen,T., Ye,Z., Lin,K., Sun,Y., Xu,J. and Liang,X.
  TITLE     Circ-RAPGEF5 promotes intrahepatic cholangiocarcinoma progression
            by stabilizing SAE1 to facilitate SUMOylation
  JOURNAL   J Exp Clin Cancer Res 42 (1), 239 (2023)
   PUBMED   37705041
  REMARK    GeneRIF: Circ-RAPGEF5 promotes intrahepatic cholangiocarcinoma
            progression by stabilizing SAE1 to facilitate SUMOylation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6916)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 6916)
  AUTHORS   Yang,X., Coulombe-Huntington,J., Kang,S., Sheynkman,G.M., Hao,T.,
            Richardson,A., Sun,S., Yang,F., Shen,Y.A., Murray,R.R., Spirohn,K.,
            Begg,B.E., Duran-Frigola,M., MacWilliams,A., Pevzner,S.J.,
            Zhong,Q., Wanamaker,S.A., Tam,S., Ghamsari,L., Sahni,N., Yi,S.,
            Rodriguez,M.D., Balcha,D., Tan,G., Costanzo,M., Andrews,B.,
            Boone,C., Zhou,X.J., Salehi-Ashtiani,K., Charloteaux,B., Chen,A.A.,
            Calderwood,M.A., Aloy,P., Roth,F.P., Hill,D.E., Iakoucheva,L.M.,
            Xia,Y. and Vidal,M.
  TITLE     Widespread Expansion of Protein Interaction Capabilities by
            Alternative Splicing
  JOURNAL   Cell 164 (4), 805-817 (2016)
   PUBMED   26871637
REFERENCE   6  (bases 1 to 6916)
  AUTHORS   Murea,M., Lu,L., Ma,L., Hicks,P.J., Divers,J., McDonough,C.W.,
            Langefeld,C.D., Bowden,D.W. and Freedman,B.I.
  TITLE     Genome-wide association scan for survival on dialysis in
            African-Americans with type 2 diabetes
  JOURNAL   Am J Nephrol 33 (6), 502-509 (2011)
   PUBMED   21546767
REFERENCE   7  (bases 1 to 6916)
  AUTHORS   Hwang,S.J., Yang,Q., Meigs,J.B., Pearce,E.N. and Fox,C.S.
  TITLE     A genome-wide association for kidney function and endocrine-related
            traits in the NHLBI's Framingham Heart Study
  JOURNAL   BMC Med Genet 8 Suppl 1 (Suppl 1), S10 (2007)
   PUBMED   17903292
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 6916)
  AUTHORS   Rebhun,J.F., Castro,A.F. and Quilliam,L.A.
  TITLE     Identification of guanine nucleotide exchange factors (GEFs) for
            the Rap1 GTPase. Regulation of MR-GEF by M-Ras-GTP interaction
  JOURNAL   J Biol Chem 275 (45), 34901-34908 (2000)
   PUBMED   10934204
REFERENCE   9  (bases 1 to 6916)
  AUTHORS   de Rooij,J., Rehmann,H., van Triest,M., Cool,R.H., Wittinghofer,A.
            and Bos,J.L.
  TITLE     Mechanism of regulation of the Epac family of cAMP-dependent
            RapGEFs
  JOURNAL   J Biol Chem 275 (27), 20829-20836 (2000)
   PUBMED   10777494
REFERENCE   10 (bases 1 to 6916)
  AUTHORS   Ichiba,T., Hoshi,Y., Eto,Y., Tajima,N. and Kuraishi,Y.
  TITLE     Characterization of GFR, a novel guanine nucleotide exchange factor
            for Rap1
  JOURNAL   FEBS Lett 457 (1), 85-89 (1999)
   PUBMED   10486569
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC005079.6.
            
            On May 17, 2019 this sequence version replaced NM_012294.4.
            
            Summary: Members of the RAS (see HRAS; MIM 190020) subfamily of
            GTPases function in signal transduction as GTP/GDP-regulated
            switches that cycle between inactive GDP- and active GTP-bound
            states. Guanine nucleotide exchange factors (GEFs), such as
            RAPGEF5, serve as RAS activators by promoting acquisition of GTP to
            maintain the active GTP-bound state and are the key link between
            cell surface receptors and RAS activation (Rebhun et al., 2000
            [PubMed 10934204]).[supplied by OMIM, Mar 2008].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000665637.1/ ENSP00000499535.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-325               AC005079.6         406863-407187       c
            326-376             AC005079.6         368020-368070       c
            377-483             AC005079.6         365403-365509       c
            484-605             AC005079.6         360002-360123       c
            606-774             AC005079.6         358372-358540       c
            775-841             AC005079.6         341208-341274       c
            842-890             AC005079.6         316997-317045       c
            891-964             AC005079.6         280879-280952       c
            965-1090            AC005079.6         269899-270024       c
            1091-1209           AC005079.6         243947-244065       c
            1210-1298           AC005079.6         243399-243487       c
            1299-1377           AC005079.6         217102-217180       c
            1378-1522           AC005079.6         212429-212573       c
            1523-1620           AC005079.6         210550-210647       c
            1621-1651           AC005079.6         207887-207917       c
            1652-1730           AC005079.6         206842-206920       c
            1731-1880           AC005079.6         204487-204636       c
            1881-1978           AC005079.6         200439-200536       c
            1979-2101           AC005079.6         196929-197051       c
            2102-2280           AC005079.6         195076-195254       c
            2281-2371           AC005079.6         190057-190147       c
            2372-2422           AC005079.6         186965-187015       c
            2423-2510           AC005079.6         186070-186157       c
            2511-2575           AC005079.6         181069-181133       c
            2576-2630           AC005079.6         175636-175690       c
            2631-6916           AC005079.6         168268-172553       c
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p15.3"
     gene            1..6916
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /note="Rap guanine nucleotide exchange factor 5"
                     /db_xref="GeneID:9771"
                     /db_xref="HGNC:HGNC:16862"
                     /db_xref="MIM:609527"
     exon            1..325
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     CDS             95..2746
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /note="isoform 1 is encoded by transcript variant 1;
                     guanine nucleotide exchange factor for Rap1;
                     M-Ras-regulated GEF; related to Epac; M-Ras-regulated Rap
                     GEF; Rap guanine nucleotide exchange factor (GEF) 5"
                     /codon_start=1
                     /product="rap guanine nucleotide exchange factor 5 isoform
                     1"
                     /protein_id="NP_036426.4"
                     /db_xref="CCDS:CCDS55093.2"
                     /db_xref="GeneID:9771"
                     /db_xref="HGNC:HGNC:16862"
                     /db_xref="MIM:609527"
                     /translation="MRMAVGSVKMQPPCESPALAAAAAVVAADGPLRRSPSAREPERE
                     QPPASLRPRLRDLPALLRSGLTLRRKRSAAGGRTLSRRISNPYLEHTPSQIYGENSSC
                     AGRALRNIIIVQAADLIKDRVNLKGFYRRSCVGSELVDWLLEHCPFVQCRSMAIGVWQ
                     LLLDMGIMLSVDQHLYFQDTYVFYQFSSDECSYLYCEFEREEEWQNGVKLLLQLVPLI
                     PARGGICELSHQKIEDSEESSDEILVRLTSAVQRELAAVIALKARKSAIEQDEENNDK
                     HVAVTEAESVPDSQAGVMCKLQERDEIGRIELVQKLAKENYQFLQTDKKEQEKSEHQD
                     DEVTTVQVKEQDQSVLVLKKVQCCGPAPTAGSAESHWRYVVVSGTPEKILEHLLNDLH
                     LEEVQDKETETLLDDFLLTYTVFMTTDDLCQALLRHYSAKKYQGKEENSDVPRRKRKV
                     LHLVSQWIALYKDWLPEDEHSKMFLKTIYRNVLDDVYEYPILEKELKEFQKILGMHRR
                     HTVDEYSPQKKNKALFHQFSLKENWLQHRGTVTETEEIFCHVYITEHSYVSVKAKVSS
                     IAQEILKVVAEKIQYAEEDLALVAITFSGEKHELQPNDLVISKSLEASGRIYVYRKDL
                     ADTLNPFAENEESQQRSMRILGMNTWDLALELMNFDWSLFNSIHEQELIYFTFSRQGS
                     GEHTANLSLLLQRCNEVQLWVATEILLCSQLGKRVQLVKKFIKIAAHCKAQRNLNSFF
                     AIVMGLNTASVSRLSQTWEKIPGKFKKLFSELESLTDPSLNHKAYRDAFKKMKPPKIP
                     FMPLLLKDVTFIHEGNKTFLDNLVNFEKLHMIADTVRTLRHCRTNQFGDLSPKEHQEL
                     KSYVNHLYVIDSQQALFELSHRIEPRV"
     exon            326..376
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            377..483
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            484..605
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            606..774
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            775..841
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            842..890
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            891..964
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1090
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            1091..1209
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            1210..1298
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1377
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            1378..1522
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            1523..1620
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1651
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            1652..1730
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            1731..1880
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            1881..1978
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            1979..2101
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            2102..2280
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            2281..2371
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            2372..2422
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            2423..2510
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            2511..2575
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            2576..2630
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     exon            2631..6916
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      6849
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /note="major polyA site"
     regulatory      6892..6897
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
                     /note="hexamer: ATTAAA"
     polyA_site      6916
                     /gene="RAPGEF5"
                     /gene_synonym="GFR; MR-GEF; MRGEF; REPAC"
ORIGIN      
        1 agtcgcgccc tctcgcttcc tggcgaaggc gcggacggcg cgcggaggcg agcgcggtgg
       61 agaggagccc ccggcgccgc agcgccgtca gggcatgagg atggccgtgg gctccgtcaa
      121 gatgcagccg ccgtgcgaga gcccggccct ggccgccgcg gcggcggtgg tggcggcaga
      181 cggccccctg cgccgcagcc ccagcgcccg ggagcccgag cgcgagcagc cgccggcgtc
      241 gctgcggccg cggctgaggg acctgcccgc gctgctgcgg agcgggctca cgctgcggag
      301 gaagcggagc gccgctgggg gccggactct atcaagaaga atatctaatc cgtaccttga
      361 acatacgcct tcccagattt atggagagaa ttcttcttgt gcaggaagag cattgaggaa
      421 tattattatc gttcaagcag ctgacctgat aaaggacaga gtgaacctca aggggtttta
      481 caggaggagc tgcgttgggt cagagctggt agactggctt ctagaacact gtcctttcgt
      541 ccagtgcaga tctatggcca taggagtctg gcaactccta ctggacatgg gaattatgtt
      601 atcagtggac cagcatctat actttcaaga tacttatgtt ttctaccagt tttcctctga
      661 tgaatgtagc tacttgtact gtgaatttga aagagaagaa gaatggcaaa atggtgtcaa
      721 gcttttactg caacttgtgc ctctcattcc tgccagaggt ggcatctgtg aactgtctca
      781 tcagaaaatt gaagactccg aagaaagcag tgatgaaatt cttgtgcgtc taacatctgc
      841 ggtgcagaga gagctagcag ctgttattgc tttgaaagca aggaagtctg caattgaaca
      901 agatgaagaa aacaacgaca aacatgtagc tgtaacagaa gccgaaagtg ttccagattc
      961 tcaggcaggg gtgatgtgca agctccagga aagagatgaa atcggacgaa ttgaactagt
     1021 ccagaagctg gcaaaagaaa actatcagtt tttgcagacg gacaaaaaag aacaggagaa
     1081 gtctgaacac caagatgatg aagtgacgac tgttcaggtt aaagagcaag accagagcgt
     1141 cctggtgctg aagaaagtgc agtgctgtgg cccagccccc acagctggga gtgcggagag
     1201 ccattggaga tatgtggtgg tgtccgggac cccggagaag attttggagc accttttgaa
     1261 tgacttgcac ctggaagaag tccaggacaa agaaacagag accctcctgg atgacttcct
     1321 tctcacgtac actgtcttca tgacaactga tgacttgtgc caggctctgt taaggcacta
     1381 ttctgctaag aagtatcaag gcaaagagga aaactcagac gttccgcgta ggaaacgtaa
     1441 agtcttgcat cttgtttccc agtggattgc tctgtacaaa gactggttac ctgaagatga
     1501 acattcaaaa atgtttttaa agaccatata taggaatgta ctggatgatg tttatgaata
     1561 tccaatactt gaaaaagaat tgaaagaatt tcaaaagata cttggaatgc accgtcgtca
     1621 cactgtagat gaatattcac cacaaaaaaa gaataaagcc cttttccacc aattcagtct
     1681 taaggagaac tggctccagc atagaggaac tgtgactgaa acggaggaaa ttttctgcca
     1741 cgtgtatata acagagcact cctatgtcag tgtgaaggca aaagtttcca gtatagccca
     1801 agagatccta aaagtcgtgg cagaaaagat ccagtatgca gaagaggatc tggctctggt
     1861 ggccatcaca ttctctgggg aaaagcatga acttcagcca aatgacttag tcatctccaa
     1921 atccctcgag gcatctggtc gaatatatgt ctaccggaaa gacctggcgg acactttgaa
     1981 cccatttgca gaaaatgagg aatcacagca aaggtcgatg aggattttgg gaatgaacac
     2041 ttgggatctt gctctggaat taatgaattt tgattggagt ctattcaatt caattcacga
     2101 gcaagagctg atctacttca cgttcagcag acagggaagt ggggaacaca ctgcaaatct
     2161 cagccttctg ctccagagat gcaatgaggt ccagctttgg gtggccacgg agattctgct
     2221 ctgcagccag ctgggcaagc gagtgcagct ggtgaaaaaa ttcatcaaaa ttgcggctca
     2281 ctgcaaagcc cagagaaacc tgaattcttt ctttgccatt gtgatgggtc tcaacactgc
     2341 ttctgtcagt cgactgtcgc agacctggga gaaaatccct gggaagttta agaaactttt
     2401 ctctgaactt gaaagtttaa cagatccttc cctaaatcac aaagcctaca gagatgcatt
     2461 caaaaagatg aagccaccaa aaatcccttt catgccctta ttgcttaaag atgtaacatt
     2521 tattcatgaa ggaaataaaa cttttttgga taatcttgtc aattttgaaa agctgcatat
     2581 gatcgcagac actgtccgaa ccctgagaca ctgcaggact aaccagtttg gtgacctgtc
     2641 tccaaaagag catcaagagt taaagtccta tgttaatcac ctgtatgtca ttgacagcca
     2701 gcaggctctg tttgagctct cacacaggat cgagcctcgg gtgtgagccc cactgcctca
     2761 cctcccctgt atctgcagca ctttgagcta cgggaatgtc tatgccaagc acgttgcttt
     2821 cctgtgagaa aagaagttgc tgagttttat cagtataacc caagacattc acaggaaagc
     2881 cagccaaagc gtgttcagga agtgatgtca gccaccagag agggggagag gtttctccat
     2941 gctactctcg ggacaagaag gcagaaggag agtcagaagc attcttgaga tggagaaggc
     3001 tggtttctta tgatcacatt gttgatccag tccagttttc aatatgagat gtgccagcat
     3061 caagacaaga caacgtcttg acatgcaatg accaaatatt tcattaagag cgtgcatgaa
     3121 acaggaagga gtttttactt tgcctagttt tagattactg tccataagct gtcaaagaag
     3181 tcattctttt gaacacctga tgacagagac agcatctcta gatctccagg gaggagaggt
     3241 ttctgttgat acaacctgtg acatcaccaa aagccacttg tgtctaggga gttagtgagg
     3301 actgcagcta gcatccatgc tctgatgggc agatgaacaa tgtcaaggtg tgcatcactt
     3361 tgcaccacaa tcaactattg acacatgctt gcaggtgaaa ttagtttctg tacaactgat
     3421 ttgcagctat aggcaaggta gatgaagttg ctttgccagt aaggaaaaat agtaatcttt
     3481 aagaaattga ctcattgttt aatttctggg gattttcttt atacttctaa gcaggctctt
     3541 atcttttatt ggacataata tgattttgaa aaagcacagt gcctgacaca ttgcaaacac
     3601 tcaccaactg cttgctgagg tgacagagtc acaaaagtct gcattcttgt gcctgatgat
     3661 gcattttgcg tacctcatac aggctccttg cccacactat ggaatgacag cagccagtgc
     3721 agggaggtta agtgacattt aatgagtgaa gcacttagca ctctctaggt aataagatag
     3781 tggtaattac tagtgttttg gcaaatgaaa aatgccctga aatagccaaa tgtctgatta
     3841 atgttggcaa cttagaagtc ctataatcca actaccagcc aaagcaggga gcctttctat
     3901 aatttgcctt tttttttttt tttttcaaaa tctgagtctt ctaaaatctt attattccca
     3961 tttttaccaa ttgaggctcc tgtagcaaat aagacctctt gatattttca aggactggtt
     4021 agaggatttc tttcaacctt cacatgaaca aaacagccta tgggtcaaaa taatgaaatc
     4081 cacccctgcc tgctagatac ttgtcacctt gctaaaatgc aagggcctgg tccattcatt
     4141 ttccaaatgc aggagtcttg gtgcacttct cactcttcct gcctgttcat ctctttcatg
     4201 cccacacaga cctgtttcct ttttgtctca tcaacgcctc attcatcctc attactgagg
     4261 cgtgtccaat gctttttgac atctttatag cagtgctgtt tcctgggctc aggaaccaca
     4321 ctgagcttga gatactgctg gaaggaacca tgtggagaga aggtttggga gaactttgag
     4381 agagacttag tttggcccag catgtaaaac ttcagtcctg aacatttata gggttttata
     4441 gaagggcatc ctccagggct ggtccattca gagaaatgct gcatgctgcc gtcatggaat
     4501 gtggcccaca ggacaccaga gccgtgagaa ccggagagca gacttccctc acggctgggc
     4561 tgagcaaacc ctccaaagcc ctcctcacgc agttactaac aatagcatgg gcttacagca
     4621 caagcacgtg ttctcacctt tttcctatgc cctggactaa ggtttggcca gtgtaatcat
     4681 ataaggccat cctgacattg tttctgtgtt tcaaaatttg gatttttatt tacattagaa
     4741 ctacattgct cctagtagaa cattaccttt aggggactaa ttttccatgg agaactattt
     4801 cagcatattg catgctgctc agaccccaag tcagatatgc ccaccaagcc agatgaagct
     4861 acacaaatgt ggtatttaaa tgcattttgt acagtgactt cagagtatct cacatgacat
     4921 gggtgtaaac tggctgggga gaaaatgatg cttgttcacc tcttcctcca gccgtggtta
     4981 ggtggtccta ggggtagcag agggaaggga ggattttgtg cagtcaagat ttgcttttcc
     5041 atccttgtct tctgaatgtc taaaatctct gcatctttct gaagtttaac aactgtctcc
     5101 agaggtttgc caggcagcag ctctcagaag tttccaaagc tttgcagaat cttagatctg
     5161 gaattaaaga attcaagccc gaattgtgag aaccagatat tactcaacag aaagctcttt
     5221 ctaaggaatc tgagctgttc actggtggac agtggtgggg cttgagtgct ccttgttaat
     5281 aggatgggcc atgcaccctc tctggatatt caccaaggcc tcttcagaat agggtttgtt
     5341 ctggctagaa gcgtggtcta gaagatggct aagctctttg ccagctctca tttggagttt
     5401 tattattgca taaaatcttc gctcactctg caaatcttac gtaatctggc accttcggca
     5461 ccaggtggtg caggggcact tctaagtggg ctctttttgt tacagcacaa ctctcagaca
     5521 gtcctgtggg tctttggatt cgtcagcatt ccagcaaact agccctgctt agaagttagc
     5581 acaagacagc agaatgcagg accccgtagg caaaatcaca accttgctat taaaaaaaat
     5641 tttgttttac atacacattt gcaggtgttc cctagagtgt ggtgttttga atttgctctt
     5701 tgtcatctgt ataattgcca aatgattata gtgatacaca tgacctgcat tcactttttt
     5761 ctagtttcct taattatgtt tagaataaat tcatttccct agaccgagaa ccacaaacag
     5821 gtagtgtgga gcatacaccg aatttagaag catgtggata aggtcagtgc tcacactgcc
     5881 tagtccacag ggagaggatg ctgcatgaat atatacttgc ctctgagtgg aggagaaatc
     5941 gtggcatgaa agagagagta ccagtgatga cttcttatcc ctggagctgg gctttcactg
     6001 ctacccatat cccagccctg cgagtctgtt ctagccagca cagacaccgc agatccggaa
     6061 ctgaatgttc ctaaatggcg cagccaatcc aggcttttca gaaactgggc aaaaacatta
     6121 aaatggggac gatcgggtct tccgcagtgg tccaacacag gatttctttt aaatgtttca
     6181 aaaacatgtc cttaaaattt cagcctgctt cttagcgagt gggccagttt tgcttaaaac
     6241 tggtgggggg gcggggggga agtttttaaa aattgccaaa aagttagatg caaatgtatt
     6301 actgtataaa gcaaagctgt atatactaaa cattttttag cagagtaata tttatttgca
     6361 tagtctattt attgtattcg tatcacactg ttattaaata ctgggatgaa atcagtgacc
     6421 tgaagcaaga aatcttgcct tttaatgtat cattaattag ggctgctgtg atattgtcag
     6481 cttgcattaa caattagaag atagagaacc cgccatcagg gtgtctacct aacttctcag
     6541 ggactacact tggtagtttt ccaccattta aagaactggt aaatatgaaa catttgttga
     6601 gttaccagaa ttgccattaa cagtgttttc tttcccatat tccatgcttt ctgcctctgt
     6661 gtatatatat aatatatatg tatatgactg tgctgtgtat ttatcgaagc tagtaagcaa
     6721 taatttatat gtaaaaatgg ccaagcaata taaggttaaa acttatataa gtaaccctta
     6781 ccttatcttg tattttcaat ttttttttaa aactgctttt ccaaatatga gactatgtta
     6841 aagacactat tcatgcctct tagtttcttg ctttctctgt gatgtgtgtg gattaaatag
     6901 ctctgttctg gcagca
//
