LOCUS       NM_007023               4301 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens Rap guanine nucleotide exchange factor 4 (RAPGEF4),
            transcript variant 1, mRNA.
ACCESSION   NM_007023
VERSION     NM_007023.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Krishnan,A., Bhasker,A.I., Singh,M.K., Rodriguez,C.I., Perez,E.C.,
            Altameemi,S., Lares,M., Khan,H., Ndiaye,M., Ahmad,N., Schieke,S.M.
            and Setaluri,V.
  TITLE     EPAC Regulates Melanoma Growth by Stimulating mTORC1 Signaling and
            Loss of EPAC Signaling Dependence Correlates with Melanoma
            Progression
  JOURNAL   Mol Cancer Res 20 (10), 1548-1560 (2022)
   PUBMED   35834616
  REMARK    GeneRIF: EPAC Regulates Melanoma Growth by Stimulating mTORC1
            Signaling and Loss of EPAC Signaling Dependence Correlates with
            Melanoma Progression.
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Ni,Z. and Cheng,X.
  TITLE     Origin and Isoform Specific Functions of Exchange Proteins Directly
            Activated by cAMP: A Phylogenetic Analysis
  JOURNAL   Cells 10 (10), 2750 (2021)
   PUBMED   34685730
  REMARK    GeneRIF: Origin and Isoform Specific Functions of Exchange Proteins
            Directly Activated by cAMP: A Phylogenetic Analysis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4301)
  AUTHORS   Pang,W., Yao,W., Dai,X., Zhang,A., Hou,L., Wang,L., Wang,Y.,
            Huang,X., Meng,X. and Li,L.
  TITLE     Pancreatic cancer-derived exosomal microRNA-19a induces beta-cell
            dysfunction by targeting ADCY1 and EPAC2
  JOURNAL   Int J Biol Sci 17 (13), 3622-3633 (2021)
   PUBMED   34512170
  REMARK    GeneRIF: Pancreatic cancer-derived exosomal microRNA-19a induces
            beta-cell dysfunction by targeting ADCY1 and EPAC2.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4301)
  AUTHORS   Choi,E.J., Wu,W., Cong,X., Zhang,K., Luo,J., Ye,S., Wang,P.,
            Suresh,A., Ullah,U.M., Zhou,J. and Bao,X.
  TITLE     Broad Impact of Exchange Protein Directly Activated by cAMP 2
            (EPAC2) on Respiratory Viral Infections
  JOURNAL   Viruses 13 (6), 1179 (2021)
   PUBMED   34205489
  REMARK    GeneRIF: Broad Impact of Exchange Protein Directly Activated by
            cAMP 2 (EPAC2) on Respiratory Viral Infections.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4301)
  AUTHORS   Lee,K.
  TITLE     Epac: new emerging cAMP-binding protein
  JOURNAL   BMB Rep 54 (3), 149-156 (2021)
   PUBMED   33298248
  REMARK    GeneRIF: Epac: new emerging cAMP-binding protein.
            Review article
REFERENCE   6  (bases 1 to 4301)
  AUTHORS   Kashima,Y., Miki,T., Shibasaki,T., Ozaki,N., Miyazaki,M., Yano,H.
            and Seino,S.
  TITLE     Critical role of cAMP-GEFII--Rim2 complex in incretin-potentiated
            insulin secretion
  JOURNAL   J Biol Chem 276 (49), 46046-46053 (2001)
   PUBMED   11598134
REFERENCE   7  (bases 1 to 4301)
  AUTHORS   Coppola,T., Magnin-Luthi,S., Perret-Menoud,V., Gattesco,S.,
            Schiavo,G. and Regazzi,R.
  TITLE     Direct interaction of the Rab3 effector RIM with Ca2+ channels,
            SNAP-25, and synaptotagmin
  JOURNAL   J Biol Chem 276 (35), 32756-32762 (2001)
   PUBMED   11438518
REFERENCE   8  (bases 1 to 4301)
  AUTHORS   de Rooij,J., Rehmann,H., van Triest,M., Cool,R.H., Wittinghofer,A.
            and Bos,J.L.
  TITLE     Mechanism of regulation of the Epac family of cAMP-dependent
            RapGEFs
  JOURNAL   J Biol Chem 275 (27), 20829-20836 (2000)
   PUBMED   10777494
REFERENCE   9  (bases 1 to 4301)
  AUTHORS   Kawasaki,H., Springett,G.M., Mochizuki,N., Toki,S., Nakaya,M.,
            Matsuda,M., Housman,D.E. and Graybiel,A.M.
  TITLE     A family of cAMP-binding proteins that directly activate Rap1
  JOURNAL   Science 282 (5397), 2275-2279 (1998)
   PUBMED   9856955
REFERENCE   10 (bases 1 to 4301)
  AUTHORS   Kawasaki,H., Springett,G.M., Toki,S., Canales,J.J., Harlan,P.,
            Blumenstiel,J.P., Chen,E.J., Bany,I.A., Mochizuki,N., Ashbacher,A.,
            Matsuda,M., Housman,D.E. and Graybiel,A.M.
  TITLE     A Rap guanine nucleotide exchange factor enriched highly in the
            basal ganglia
  JOURNAL   Proc Natl Acad Sci U S A 95 (22), 13278-13283 (1998)
   PUBMED   9789079
  REMARK    Erratum:[Proc Natl Acad Sci U S A 1999 Jan 5;96(1):318]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC018712.5, AC009484.3, AC104086.6 and AC019046.4.
            
            On Nov 23, 2018 this sequence version replaced NM_007023.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.237474.1,
                                           SRR1803613.166907.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145122, SAMEA2145240
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000397081.8/ ENSP00000380271.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-204               AC018712.5         16564-16767         c
            205-347             AC009484.3         163319-163461       c
            348-436             AC009484.3         160873-160961       c
            437-583             AC009484.3         144061-144207       c
            584-656             AC009484.3         40612-40684         c
            657-676             AC009484.3         36186-36205         c
            677-730             AC104086.6         4275-4328
            731-837             AC104086.6         4637-4743
            838-959             AC104086.6         9077-9198
            960-1143            AC104086.6         10776-10959
            1144-1228           AC019046.4         168103-168187       c
            1229-1289           AC019046.4         166190-166250       c
            1290-1366           AC019046.4         163411-163487       c
            1367-1513           AC019046.4         162844-162990       c
            1514-1629           AC019046.4         160758-160873       c
            1630-1718           AC019046.4         155129-155217       c
            1719-1797           AC019046.4         150339-150417       c
            1798-1948           AC019046.4         137069-137219       c
            1949-2037           AC019046.4         135246-135334       c
            2038-2068           AC019046.4         134479-134509       c
            2069-2147           AC019046.4         134179-134257       c
            2148-2294           AC019046.4         132881-133027       c
            2295-2392           AC019046.4         130968-131065       c
            2393-2518           AC019046.4         124986-125111       c
            2519-2697           AC019046.4         124424-124602       c
            2698-2788           AC019046.4         121429-121519       c
            2789-2839           AC019046.4         117719-117769       c
            2840-2927           AC019046.4         115471-115558       c
            2928-2992           AC019046.4         114991-115055       c
            2993-3047           AC019046.4         103029-103083       c
            3048-4301           AC019046.4         98790-100043        c
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q31.1"
     gene            1..4301
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /note="Rap guanine nucleotide exchange factor 4"
                     /db_xref="GeneID:11069"
                     /db_xref="HGNC:HGNC:16626"
                     /db_xref="MIM:606058"
     exon            1..204
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     CDS             140..3175
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /note="isoform a is encoded by transcript variant 1;
                     exchange protein directly activated by cAMP 2;
                     cAMP-regulated guanine nucleotide exchange factor II; RAP
                     guanine-nucleotide-exchange factor (GEF) 4; putative
                     protein product of Nbla00496; exchange factor directly
                     activated by cAMP 2; Rap guanine nucleotide exchange
                     factor (GEF) 4"
                     /codon_start=1
                     /product="rap guanine nucleotide exchange factor 4 isoform
                     a"
                     /protein_id="NP_008954.2"
                     /db_xref="CCDS:CCDS42775.1"
                     /db_xref="GeneID:11069"
                     /db_xref="HGNC:HGNC:16626"
                     /db_xref="MIM:606058"
                     /translation="MVAAHAAHSSSSAEWIACLDKRPLERSSEDVDIIFTRLKEVKAF
                     EKFHPNLLHQICLCGYYENLEKGITLFRQGDIGTNWYAVLAGSLDVKVSETSSHQDAV
                     TICTLGIGTAFGESILDNTPRHATIVTRESSELLRIEQKDFKALWEKYRQYMAGLLAP
                     PYGVMETGSNNDRIPDKENTPLIEPHVPLRPANTITKVPSEKILRAGKILRNAILSRA
                     PHMIRDRKYHLKTYRQCCVGTELVDWMMQQTPCVHSRTQAVGMWQVLLEDGVLNHVDQ
                     EHHFQDKYLFYRFLDDEHEDAPLPTEEEKKECDEELQDTMLLLSQMGPDAHMRMILRK
                     PPGQRTVDDLEIIYEELLHIKALSHLSTTVKRELAGVLIFESHAKGGTVLFNQGEEGT
                     SWYIILKGSVNVVIYGKGVVCTLHEGDDFGKLALVNDAPRAASIVLREDNCHFLRVDK
                     EDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQGNSQPQQKYTVMSGTPEKI
                     LEHFLETIRLEATLNEATDSVLNDFIMMHCVFMPNTQLCPALVAHYHAQPSQGTEQEK
                     MDYALNNKRRVIRLVLQWAAMYGDLLQEDDVSMAFLEEFYVSVSDDARMIAALKEQLP
                     ELEKIVKQISEDAKAPQKKHKVLLQQFNTGDERAQKRQPIRGSDEVLFKVYCMDHTYT
                     TIRVPVATSVKEVISAVADKLGSGEGLIIVKMSSGGEKVVLKPNDVSVFTTLTINGRL
                     FACPREQFDSLTPLPEQEGPTVGTVGTFELMSSKDLAYQMTIYDWELFNCVHELELIY
                     HTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKRVQLLKKFIKIAAHCKE
                     YKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYRLTVA
                     KLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTARTVRYYRSQPFNPD
                     AAQANKNHQDVRSYVRQLNVIDNQRTLSQMSHRLEPRRP"
     exon            205..347
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            348..436
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            437..583
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            584..656
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            657..676
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            677..730
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            731..837
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            838..959
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1143
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1228
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1289
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            1290..1366
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            1367..1513
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            1514..1629
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            1630..1718
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            1719..1797
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            1798..1948
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            1949..2037
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            2038..2068
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            2069..2147
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            2148..2294
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            2295..2392
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            2393..2518
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            2519..2697
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            2698..2788
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            2789..2839
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            2840..2927
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            2928..2992
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            2993..3047
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     exon            3048..4301
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4281..4286
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /note="hexamer: AATATA"
     polyA_site      4301
                     /gene="RAPGEF4"
                     /gene_synonym="CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2;
                     Nbla00496"
                     /note="major polyA site"
ORIGIN      
        1 gggcctgtcg cagccgcgct ggtcgccagg cgtccgggag gagcggggtc cgcgcggcgg
       61 acgaggcggg ggcggaggcg caggcagagc gagcgcggga ggtcgccgca gccagggaca
      121 ccgcgcgccg ccgctcaaca tggtcgctgc gcacgctgcc cattcttcct cctctgccga
      181 gtggatcgcc tgcctggata aaagaccact ggagcgatcc agcgaagatg tggatataat
      241 cttcactcga ctgaaagaag ttaaagcttt tgagaaattt cacccaaatc tccttcatca
      301 gatttgctta tgtggttatt atgagaatct ggaaaaggga ataacattat ttcgccaggg
      361 tgatattgga acaaactggt atgctgtcct ggcagggtct ttggatgtta aagtatctga
      421 gaccagcagt caccaggatg ctgtgaccat ctgtaccctg ggaattggga cggcctttgg
      481 agagtccatt ctggacaaca caccccgcca tgcaaccatc gttaccaggg agagcagtga
      541 actgctccgc atcgagcaga aggacttcaa ggcactatgg gagaaatatc gacagtatat
      601 ggcaggactt ctggctcctc cttatggtgt tatggaaacg ggctctaaca atgacaggat
      661 tcctgacaag gagaacacac ctctcattga acctcacgtt cctcttcgtc ctgctaacac
      721 cattaccaag gtcccttcag agaagatcct cagagctgga aaaattttac gaaatgccat
      781 tctctctcga gcacctcaca tgataagaga tagaaaatac cacctaaaga catacagaca
      841 atgctgtgtg ggaactgaac tggtggactg gatgatgcag cagacaccat gtgttcactc
      901 ccggactcaa gctgttggca tgtggcaagt cctgttagaa gatggtgttc tcaaccacgt
      961 ggaccaggag caccatttcc aagacaaata tttattctat cgatttctgg atgatgagca
     1021 cgaggatgcc cctttgccta ctgaggagga gaagaaggag tgtgatgagg agctccagga
     1081 caccatgctg ctgctgtcac agatgggccc cgacgcccac atgaggatga tccttcgcaa
     1141 accacctggc cagaggactg tggatgacct agagattatc tatgaggagc ttcttcatat
     1201 taaagcctta tcccatcttt ctaccacagt gaaacgagag ttagcaggtg ttctcatttt
     1261 tgagtctcac gccaaaggag ggactgtgtt gtttaaccag ggggaagaag gtacctcctg
     1321 gtacattatt ctaaaaggat cagtgaatgt agtcatttac ggcaagggtg tggtctgcac
     1381 cctgcatgaa ggagatgact tcggcaagtt agcactagtg aatgatgccc cacgagctgc
     1441 ctctatcgtc ttacgagaag ataactgcca tttcttaaga gtagacaagg aggatttcaa
     1501 ccggatccta agggacgtgg aggcgaatac agtcagactt aaagaacatg accaagatgt
     1561 cttggtgctg gagaaggtcc cagcagggaa cagagcttct aatcaaggaa actcacagcc
     1621 tcagcaaaag tatactgtga tgtcaggaac acctgaaaaa attttagagc attttctaga
     1681 aacaatacgc cttgaggcaa ctttaaatga agcaacagat tctgttttaa atgactttat
     1741 tatgatgcac tgtgttttta tgccaaatac ccagctttgc ccggcactgg tggcccacta
     1801 ccacgcacag ccttcacaag gtacagaaca ggagaaaatg gattatgccc tcaacaataa
     1861 gaggcgagtc atccgcctgg ttctacagtg ggctgccatg tatggagacc tcctgcaaga
     1921 ggatgacgtg tctatggcct tcctggagga gttttatgta tctgtatcag atgatgcccg
     1981 gatgattgct gccctcaagg agcaactgcc agagttggag aagattgtca agcaaatctc
     2041 agaagatgca aaggcaccac aaaagaagca caaggttctt ttgcaacagt tcaatacggg
     2101 cgatgagaga gcccagaagc gccagcctat ccgcggctct gatgaagttc tgtttaaggt
     2161 ctattgcatg gaccacacct acacaaccat tcgggtgcca gtggccactt cggtgaagga
     2221 agtcatcagt gcagttgccg acaagctggg ctccggggag ggcctgatca tagtcaagat
     2281 gagttccgga ggagaaaagg tggtgctcaa acctaatgat gtttcagtat ttacgacgct
     2341 caccattaat ggacgcctgt ttgcttgccc gcgagagcaa ttcgattcac tgactccctt
     2401 accagaacag gaaggcccaa ctgttggaac agtgggaact tttgaactga tgagctccaa
     2461 agatttagca taccagatga caatttatga ttgggaactc ttcaactgcg tgcatgagct
     2521 ggagctaatc tatcacacat ttggaaggca taattttaaa aagaccacag caaacttgga
     2581 tttgttcctg aggagattta atgaaattca gttttgggtc gtcactgaga tctgcctttg
     2641 ttctcagctc agcaagcgtg ttcagctatt aaaaaaattt attaagatag cagcccactg
     2701 taaggagtat aaaaatctga attccttttt tgccatcgtc atgggactaa gtaacgttgc
     2761 tgtgagccgc ttggcactaa cgtgggagaa actgccaagc aagttcaaga agttctatgc
     2821 ggagtttgaa agtttaatgg acccttcaag gaaccacagg gcctacaggc tgacagtagc
     2881 taagctggaa cctcctctca tccccttcat gcctttgctc attaaagata tgacatttac
     2941 tcatgagggg aacaagacgt tcattgacaa tctagtaaac tttgaaaaaa tgcgcatgat
     3001 tgcaaatacg gccagaacag tgagatacta caggagccaa cccttcaatc ctgatgcagc
     3061 tcaagctaat aagaaccatc aggatgtccg gagttatgta cggcaattaa atgtgattga
     3121 caaccagaga actttatcac agatgtcaca cagattagag cctcgtcgac catagacatt
     3181 tcaaatgccc aaagcaacag tttgtctcca gtccacaatc tttcaaaaat gccatttatg
     3241 ctactactga ctgtattgcc actagagaat tctacaaaac aagcaaaaac acatcctgag
     3301 acacctcagg gctgcattca gcttaccagc tacctagcaa gagaaggaat tatttgacat
     3361 gcctaaagct tacacactct agcttaggaa tccccagttt gtggctaccc tgtttcattt
     3421 ccacttgtgc catcttcttt gctgaagtgt tgaataaggc ccacgtgaag agtttggtag
     3481 aaatagcctt gtcaagagaa ctagcaagcc cctgacattt ttttctaaga gtgtttatgg
     3541 gatgagatgt gctacaaatg ggatagattt ggataaaatt ttaactcaac atcttgattt
     3601 ggagcctggg gtttcgggaa ggtgttgagg aatctataga agtccaaatt ataggttgta
     3661 ttcttccatc ttcattgtct tacctctgaa ggaatagaac ctgaccacat tattatgaaa
     3721 cattatggct gtttatgtaa tttagggagg actggtctgt aatttctgaa tgtatataga
     3781 ataatattta tgtttacaat gtaacttttc aaaatttaca aatcaggatt atatacataa
     3841 gaattccact aagaaatgcc aagattctta aatttgccag cgttaaagta gaaaataaca
     3901 tttcagaaga gcacaatatg cataaaacat ttttcaaaat tgaaatattt tcctgggcat
     3961 taaaaacctt tactattggc tacaaattta ttgtacctga tgaaaacata ttttctggac
     4021 ttaaatgtta ttacaaatat cttaattttc agtaattgtt ttgcactttc aaagattgta
     4081 aatagttcat tcaatcaatg gtatggagtt atttatttgc tacataatag atactgtgcc
     4141 aaataattac tttttattta ttttatttag tacgtaattt tgaagtacat tttttcctgt
     4201 tttcacaatt agactacatt taatgtgtag gaattgtatg tatgtatatc ttctgtaaat
     4261 aacatctagt atcttcacta aatataattg tcgacaagaa a
//
