LOCUS       NM_001098531            6300 bp    mRNA    linear   PRI 12-OCT-2024
DEFINITION  Homo sapiens Rap guanine nucleotide exchange factor 3 (RAPGEF3),
            transcript variant 1, mRNA.
ACCESSION   NM_001098531
VERSION     NM_001098531.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Yang,W., Mei,F.C., Lin,W., White,M.A., Li,L., Li,Y., Pan,S. and
            Cheng,X.
  TITLE     Protein SUMOylation promotes cAMP-independent EPAC1 activation
  JOURNAL   Cell Mol Life Sci 81 (1), 283 (2024)
   PUBMED   38963422
  REMARK    GeneRIF: Protein SUMOylation promotes cAMP-independent EPAC1
            activation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6300)
  AUTHORS   Reverte-Salisa,L., Siddig,S., Hildebrand,S., Yao,X., Zurkovic,J.,
            Jaeckstein,M.Y., Heeren,J., Lezoualc'h,F., Krahmer,N. and
            Pfeifer,A.
  TITLE     EPAC1 enhances brown fat growth and beige adipogenesis
  JOURNAL   Nat Cell Biol 26 (1), 113-123 (2024)
   PUBMED   38195707
  REMARK    GeneRIF: EPAC1 enhances brown fat growth and beige adipogenesis.
REFERENCE   3  (bases 1 to 6300)
  AUTHORS   Iannucci,L.F., D'Erchia,A.M., Picardi,E., Bettio,D., Conca,F.,
            Surdo,N.C., Di Benedetto,G., Musso,D., Arrigoni,C., Lolicato,M.,
            Vismara,M., Grisan,F., Salviati,L., Milanesi,L., Pesole,G. and
            Lefkimmiatis,K.
  TITLE     Cyclic AMP induces reversible EPAC1 condensates that regulate
            histone transcription
  JOURNAL   Nat Commun 14 (1), 5521 (2023)
   PUBMED   37684224
  REMARK    GeneRIF: Cyclic AMP induces reversible EPAC1 condensates that
            regulate histone transcription.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6300)
  AUTHORS   Krishnan,A., Bhasker,A.I., Singh,M.K., Rodriguez,C.I., Perez,E.C.,
            Altameemi,S., Lares,M., Khan,H., Ndiaye,M., Ahmad,N., Schieke,S.M.
            and Setaluri,V.
  TITLE     EPAC Regulates Melanoma Growth by Stimulating mTORC1 Signaling and
            Loss of EPAC Signaling Dependence Correlates with Melanoma
            Progression
  JOURNAL   Mol Cancer Res 20 (10), 1548-1560 (2022)
   PUBMED   35834616
  REMARK    GeneRIF: EPAC Regulates Melanoma Growth by Stimulating mTORC1
            Signaling and Loss of EPAC Signaling Dependence Correlates with
            Melanoma Progression.
REFERENCE   5  (bases 1 to 6300)
  AUTHORS   Ferreira,J.F., Silva,I.A.L., Botelho,H.M., Amaral,M.D. and
            Farinha,C.M.
  TITLE     Absence of EPAC1 Signaling to Stabilize CFTR in Intestinal
            Organoids
  JOURNAL   Cells 11 (15), 2295 (2022)
   PUBMED   35892592
  REMARK    GeneRIF: Absence of EPAC1 Signaling to Stabilize CFTR in Intestinal
            Organoids.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6300)
  AUTHORS   Mei,F.C., Qiao,J., Tsygankova,O.M., Meinkoth,J.L., Quilliam,L.A.
            and Cheng,X.
  TITLE     Differential signaling of cyclic AMP: opposing effects of exchange
            protein directly activated by cyclic AMP and cAMP-dependent protein
            kinase on protein kinase B activation
  JOURNAL   J Biol Chem 277 (13), 11497-11504 (2002)
   PUBMED   11801596
  REMARK    GeneRIF: role in regulating protein kinase B activity
REFERENCE   7  (bases 1 to 6300)
  AUTHORS   de Rooij,J., Rehmann,H., van Triest,M., Cool,R.H., Wittinghofer,A.
            and Bos,J.L.
  TITLE     Mechanism of regulation of the Epac family of cAMP-dependent
            RapGEFs
  JOURNAL   J Biol Chem 275 (27), 20829-20836 (2000)
   PUBMED   10777494
REFERENCE   8  (bases 1 to 6300)
  AUTHORS   Kawasaki,H., Springett,G.M., Mochizuki,N., Toki,S., Nakaya,M.,
            Matsuda,M., Housman,D.E. and Graybiel,A.M.
  TITLE     A family of cAMP-binding proteins that directly activate Rap1
  JOURNAL   Science 282 (5397), 2275-2279 (1998)
   PUBMED   9856955
REFERENCE   9  (bases 1 to 6300)
  AUTHORS   de Rooij,J., Zwartkruis,F.J., Verheijen,M.H., Cool,R.H.,
            Nijman,S.M., Wittinghofer,A. and Bos,J.L.
  TITLE     Epac is a Rap1 guanine-nucleotide-exchange factor directly
            activated by cyclic AMP
  JOURNAL   Nature 396 (6710), 474-477 (1998)
   PUBMED   9853756
REFERENCE   10 (bases 1 to 6300)
  AUTHORS   Kawasaki,H., Springett,G.M., Toki,S., Canales,J.J., Harlan,P.,
            Blumenstiel,J.P., Chen,E.J., Bany,I.A., Mochizuki,N., Ashbacher,A.,
            Matsuda,M., Housman,D.E. and Graybiel,A.M.
  TITLE     A Rap guanine nucleotide exchange factor enriched highly in the
            basal ganglia
  JOURNAL   Proc Natl Acad Sci U S A 95 (22), 13278-13283 (1998)
   PUBMED   9789079
  REMARK    Erratum:[Proc Natl Acad Sci U S A 1999 Jan 5;96(1):318]
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC004241.2.
            
            On May 21, 2020 this sequence version replaced NM_001098531.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK092448.1, BC092404.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000449771.7/ ENSP00000395708.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-330               AC004241.2         126268-126597       c
            331-543             AC004241.2         125583-125795       c
            544-597             AC004241.2         119633-119686       c
            598-704             AC004241.2         119440-119546       c
            705-826             AC004241.2         119116-119237       c
            827-995             AC004241.2         118765-118933       c
            996-1080            AC004241.2         118058-118142       c
            1081-1141           AC004241.2         117647-117707       c
            1142-1218           AC004241.2         117458-117534       c
            1219-1365           AC004241.2         117107-117253       c
            1366-1478           AC004241.2         116536-116648       c
            1479-1567           AC004241.2         116171-116259       c
            1568-1646           AC004241.2         115791-115869       c
            1647-1797           AC004241.2         115429-115579       c
            1798-1880           AC004241.2         115261-115343       c
            1881-1920           AC004241.2         114577-114616       c
            1921-2002           AC004241.2         111704-111785       c
            2003-2149           AC004241.2         111247-111393       c
            2150-2247           AC004241.2         109222-109319       c
            2248-2373           AC004241.2         108632-108757       c
            2374-2555           AC004241.2         108359-108540       c
            2556-2646           AC004241.2         108022-108112       c
            2647-2697           AC004241.2         107858-107908       c
            2698-2785           AC004241.2         106860-106947       c
            2786-2850           AC004241.2         106407-106471       c
            2851-2905           AC004241.2         105910-105964       c
            2906-2977           AC004241.2         105739-105810       c
            2978-6300           AC004241.2         102080-105402       c
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q13.11"
     gene            1..6300
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /note="Rap guanine nucleotide exchange factor 3"
                     /db_xref="GeneID:10411"
                     /db_xref="HGNC:HGNC:16629"
                     /db_xref="MIM:606057"
     exon            1..330
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     CDS             325..3096
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /note="isoform a is encoded by transcript variant 1;
                     exchange protein directly activated by cAMP 1;
                     9330170P05Rik; Rap1 guanine-nucleotide-exchange factor
                     directly activated by cAMP; EPAC 1; exchange factor
                     directly activated by cAMP 1; cAMP-regulated guanine
                     nucleotide exchange factor I; Rap guanine nucleotide
                     exchange factor (GEF) 3"
                     /codon_start=1
                     /product="rap guanine nucleotide exchange factor 3 isoform
                     a"
                     /protein_id="NP_001092001.2"
                     /db_xref="CCDS:CCDS41775.1"
                     /db_xref="GeneID:10411"
                     /db_xref="HGNC:HGNC:16629"
                     /db_xref="MIM:606057"
                     /translation="MKVGWPGESCWQVGLAVEDSPALGAPRVGALPDVVPEGTLLNMV
                     LRRMHRPRSCSYQLLLEHQRPSCIQGLRWTPLTNSEESLDFSESLEQASTERVLRAGR
                     QLHRHLLATCPNLIRDRKYHLRLYRQCCSGRELVDGILALGLGVHSRSQVVGICQVLL
                     DEGALCHVKHDWAFQDRDAQFYRFPGPEPEPVRTHEMEEELAEAVALLSQRGPDALLT
                     VALRKPPGQRTDEELDLIFEELLHIKAVAHLSNSVKRELAAVLLFEPHSKAGTVLFSQ
                     GDKGTSWYIIWKGSVNVVTHGKGLVTTLHEGDDFGQLALVNDAPRAATIILREDNCHF
                     LRVDKQDFNRIIKDVEAKTMRLEEHGKVVLVLERASQGAGPSRPPTPGRNRYTVMSGT
                     PEKILELLLEAMGPDSSAHDPTETFLSDFLLTHRVFMPSAQLCAALLHHFHVEPAGGS
                     EQERSTYVCNKRQQILRLVSQWVALYGSMLHTDPVATSFLQKLSDLVGRDTRLSNLLR
                     EQWPERRRCHRLENGCGNASPQMKARNLPVWLPNQDEPLPGSSCAIQVGDKVPYDICR
                     PDHSVLTLQLPVTASVREVMAALAQEDGWTKGQVLVKVNSAGDAIGLQPDARGVATSL
                     GLNERLFVVNPQEVHELIPHPDQLGPTVGSAEGLDLVSAKDLAGQLTDHDWSLFNSIH
                     QVELIHYVLGPQHLRDVTTANLERFMRRFNELQYWVATELCLCPVPGPRAQLLRKFIK
                     LAAHLKEQKNLNSFFAVMFGLSNSAISRLAHTWERLPHKVRKLYSALERLLDPSWNHR
                     VYRLALAKLSPPVIPFMPLLLKDMTFIHEGNHTLVENLINFEKMRMMARAARMLHHCR
                     SHNPVPLSPLRSRVSHLHEDSQVARISTCSEQSLSTRSPASTWAYVQQLKVIDNQREL
                     SRLSRELEP"
     exon            331..543
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            544..597
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            598..704
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            705..826
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            827..995
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            996..1080
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            1081..1141
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            1142..1218
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..1365
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            1366..1478
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            1479..1567
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            1568..1646
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..1797
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            1798..1880
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            1881..1920
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            1921..2002
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            2003..2149
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            2150..2247
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            2248..2373
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            2374..2555
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            2556..2646
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            2647..2697
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            2698..2785
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            2786..2850
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            2851..2905
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            2906..2977
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     exon            2978..6300
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3387..3392
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /note="hexamer: AATAGA"
     polyA_site      3407
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /note="major polyA site"
     regulatory      5973..5978
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /note="hexamer: AATAAT"
     polyA_site      5993
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
     regulatory      6275..6280
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
                     /note="hexamer: TATAAA"
     polyA_site      6300
                     /gene="RAPGEF3"
                     /gene_synonym="bcm910; CAMP-GEFI; EPAC; EPAC1; HSU79275"
ORIGIN      
        1 agcgcagcct tgcttcagcg cccggtgcca gctggctcct tcgtctgaga cgaaggcgca
       61 ggctcggggc tccctgtcgc cggtggctgg gtggcgtccc cccaccacgc ccccacccct
      121 ctactcaccc aagaggagcc tctccaccga gccctgcatc ctgctgccct ggcagccagt
      181 ccagctaggc actggcccag ttcactgtac ctggacttac ccactggcac cctccggatc
      241 cccttatcaa agctgatggg ggtcgctggg gaccccccag ccttttgcta gagcctgcac
      301 ggctgtgcga gcttgaaaag aaacatgaag gtgggctggc caggtgagag ctgctggcag
      361 gtgggcctgg ctgtggagga tagcccagct ctgggagcac cgcgggtggg agccctccct
      421 gacgtggtgc cggaggggac actactcaac atggtgttga gaaggatgca ccggccccga
      481 agctgctcct accagctgct gctggagcac cagcgtccga gctgcatcca ggggctgcgc
      541 tggacaccac tcaccaacag cgaggagtcc ctggatttca gcgagagcct ggagcaggcc
      601 tccacagagc gggtgctcag ggctgggagg cagctgcatc ggcatctgct ggccacctgc
      661 ccaaacctca tccgagaccg gaagtaccac cttaggctct atcggcagtg ctgctctggc
      721 cgggagctgg tggatgggat cttggccctg ggacttgggg tccattcccg gagccaagtt
      781 gtgggaatct gccaggtgct gctggatgaa ggtgccctct gccatgtgaa acacgactgg
      841 gccttccagg accgagatgc ccaattctac cggttccccg ggcccgagcc cgagcccgtg
      901 agaactcatg agatggagga ggagttggcc gaagctgtgg ccctgctctc ccagcggggg
      961 cctgacgccc tgctcactgt ggcacttcga aagcccccag gtcagcgcac ggatgaagag
     1021 ctggacctca tctttgagga gctgctgcac atcaaggctg tggcccacct ctccaactcg
     1081 gtgaagcgag aattagcggc tgttctgctc tttgaaccac acagcaaggc agggaccgtg
     1141 ttgttcagcc agggggacaa gggcacttcg tggtacatta tctggaaggg atctgtcaac
     1201 gtggtgaccc atggcaaggg gctggtgacc accctgcatg agggagatga ttttggacag
     1261 ctggctctgg tgaatgatgc accccgggca gccaccatca tcctgcgaga agacaactgt
     1321 catttcctgc gtgtggacaa gcaggacttc aaccgtatca tcaaggatgt ggaggcaaag
     1381 accatgcggc tggaagaaca tggcaaagtg gtgctggtgc tggagagagc ctctcagggc
     1441 gccggccctt cccgaccccc aaccccaggc aggaaccggt atacagtgat gtctggcacc
     1501 ccagagaaga tcctagagct tctgttggag gccatgggac cagattccag tgctcatgac
     1561 ccaacagaga cattcctcag cgacttcctc ctgacccaca gggtcttcat gcccagcgcc
     1621 caactctgcg ctgcccttct gcaccacttc catgtggagc ctgcgggtgg cagcgagcag
     1681 gagcgcagca cctacgtctg caacaagagg cagcagatct tgcggctggt cagccagtgg
     1741 gtggccctgt atggctccat gctccacact gaccctgtgg ccaccagctt cctccagaaa
     1801 ctctcagacc tggtgggcag ggacacccga ctcagcaacc tgctgaggga gcagtggcca
     1861 gagaggcggc gatgccacag gttggagaat ggctgtggga atgcatctcc tcagatgaag
     1921 gcccggaact tgcctgtttg gctccccaac caggacgagc cccttcctgg cagcagctgt
     1981 gccatccaag ttggggataa agtcccctat gacatctgcc ggccagacca ctcagtgttg
     2041 accctgcagc tgcctgtgac agcctccgtg agagaggtga tggcagcgtt ggcccaggag
     2101 gatggctgga ccaaggggca ggtgctggtg aaggtcaatt ctgcaggtga tgccattggc
     2161 ctgcagccag atgcccgtgg tgtggccaca tctctggggc tcaatgagcg tctctttgtt
     2221 gtcaacccac aggaagtgca tgagctgatc ccacaccctg accagctggg gcccactgtg
     2281 ggctctgctg aggggctgga cctggtgagt gccaaggacc tggcaggcca gctgacggac
     2341 cacgactgga gcctcttcaa cagtatccac caggtggagc tgatccacta tgtgctgggc
     2401 ccccagcatc tgcgggatgt caccaccgcc aacctggagc gcttcatgcg ccgcttcaat
     2461 gagctgcagt actgggtggc caccgagctg tgtctctgcc ccgtgcccgg cccccgggcc
     2521 cagctgctca ggaagttcat taagctggcg gcccacctca aggagcagaa gaatctcaat
     2581 tccttctttg ccgtcatgtt tggcctcagc aactcggcca tcagccgcct agcccacacc
     2641 tgggagcggc tgcctcacaa agtccggaag ctgtactccg ccctcgagag gctgctggat
     2701 ccctcatgga accaccgggt ataccgactg gccctcgcca agctctcccc tcctgtcatc
     2761 cccttcatgc cccttcttct caaagacatg accttcattc atgagggaaa ccacacacta
     2821 gtggagaatc tcatcaactt tgagaagatg agaatgatgg ccagagccgc gcggatgctg
     2881 caccactgcc gaagccacaa ccctgtgcct ctctcaccac tcagaagccg agtttcccac
     2941 ctccacgagg acagccaggt ggcgaggatt tccacatgct cggagcagtc cctgagcacc
     3001 cggagtccag ccagcacctg ggcttatgtc cagcagctga aggtcattga caaccagcgg
     3061 gaactctccc gcctctcccg agagctggag ccatgaggag gggctgggac tggagctgga
     3121 gcaggcactt gcagccggga aagccagggt gtgccgggcc aagatactca caggctggcc
     3181 acagctgggc aaggctctcc gtggagtgga ctcgagtccc tggagcaggc agtgtggagg
     3241 cagccatccc ctgtgatgac tggcagctaa ggaggacctc ggagtggacc cgagccagga
     3301 ataacgaatg acccaaggcc aaggaaggga ggacagagag gccccaggag tgggtggaga
     3361 gtggagtgcg ctgggacgtt gtgtgcaata gagaggtctc cacaccagat gtcttccaga
     3421 ttctgtgcct ctggctttgt tgtccagcca ggcctgcagt ttattttcac agtggacaga
     3481 gagagagaga gaggctgcat gtgtgtaccg tgtgtggcaa gggcagggcc ttggcctggg
     3541 gcaggggccc cctgctttct ttccacagct ttcttccaac agcaggcagt ggggctgcgg
     3601 gcctgtgcgg tggtccctga ggagctttat tgttactgtc aacatagtag tatgttccag
     3661 gccagcccag agtgggcgtg gcagggaggc tgaggccccg ccctctggga aggagctgtg
     3721 gaggagaagc agccccatgc tgggcacggg ccagactggg tccaagatcc tgatgccacc
     3781 cagatgtgcc tccagggctc tggccaaagg gcgggctgga gcctggagcc cctgtcagca
     3841 gccagccagg gtgaccatca gctccccgtg gtggtccaat aggactttac agtccagatg
     3901 caactgacag cttcttccca actgcaatcc ccgcttttcc acacttgagc tcgtcctggc
     3961 ctgttgggcc aggcttctgg ggtcttctgg gggtggcttg tgatagaaac aatgctgaag
     4021 cttccgcgga gagcccgtcg cttgttgagg cccaacagac ttccaggaaa tcagggttta
     4081 tcatcagtgt tgagaatggt cccagcaagc tctggtcata cgaagtcctc tcttccctgc
     4141 cccttcagca cttcccctag gaaccagcta ctgccaggac gcctcttgcg aggcagagct
     4201 cacccaggaa gctccaggga ctccaccctc ttgccttgct attgccctga gtcctggcag
     4261 caggaggcac ttgtctggcc agagagagaa ctcatggccc ctggcatgac tcctgagtag
     4321 gtattgaggc tgcagggcct tgggcgtcca gaggcagctg gtatggcccc tctgcacttc
     4381 agccccatgg atgtggatac acaggcctgc tgcttccaga aagggtcaga gcctttagca
     4441 gggaggcagc acagctgggc ttagacctca ggtcactaag tgaatctgag caagtcagtg
     4501 aacagttctc ttccttcatt tctccacctg taagccaggt atgataaacg ccatacagcg
     4561 cttagtgaca aacacataaa actgtagctc ctgcctctgt agcagaggtg acagccaagg
     4621 gcagtcctgt gctatccagg cctggtaggg gggcagcctc gcctcagttg agggctgagg
     4681 gagagactga ggtgcagcag atggcagtgg taaccaaacc agttcccttg ccgtgggtgg
     4741 ggacacagga gaagcacgga agcatctgga actgagcaat caggacagga ggctcaagcc
     4801 tgggtgagtc aggagtccgg gggctttctt ttcatccaac ccattgtctg ctggtgtctc
     4861 ggcaaaagcc agccagccag ccgtggccct tcaccgttct tcagactcta gggtgagccc
     4921 agcccagcga cacaacagaa accaagtgcc acgggggccg gtgtcctcca ccagcatttc
     4981 tcctctggtc acatctacgt cctgatggga cttctccctc ctccttggac catcagcttt
     5041 acagtccaga ccaccctgca gccacccggt ggtctcccag ctgccccagt ctcgggcagg
     5101 atggcttttg agcctgttgg gagagacctt gccaggagaa tggtgcccag ggcaggcaag
     5161 aggacacaga ctctgggagc tagaagagtt gcggcccagg gggccaggcc actcccagag
     5221 gacaggaggc ccaaaagcgg ggaaaggctc catgttacgg tggccccatg ctgggagttt
     5281 gtgctgcctt ctgtctccct gactgcccaa gcgtggggtg gtgtggggca ggaggcatcc
     5341 tctggtgttc cctagcagcc caggctgtgt gcaggggctc ccattttgtg tctcagctgc
     5401 aagaaggcca caaagggagg aggggctcat gggactgctg agaagaaggt gtgcagaaag
     5461 ggagtgtggc cctagatcag tcctttaaaa agcctctgtt acctgaaagg aggtcaagca
     5521 gagaggaatg gctgcgtgtg tgtcctaccg ggcaggacag gacagaggga ggtcattgtt
     5581 acacacacat cccccctcct ttcctccttc ccctccactt ccctgtgaag gcctgaggca
     5641 ggctgtggcc aggctcaggc cctgactgtg gggtacacac cagggagggc cacgcctccc
     5701 cacagacggg gtggagtggt cagtggagcc agcaccctct gctgcttcag catctccgcc
     5761 tggagccagc tgtcccctct ctctcaggct ccatccgaac aaaaggaagg cagagcctga
     5821 gatccggcac cccatccccc tccactcagc actgagttag agctgggtgg gggcatgtct
     5881 gccttcacac gctgccttat ggcttctgtg tctccaacag ccctcagcct ctggctgctc
     5941 tccttcccaa tagtcacaag atttaccatg ataataatag gttaagactt gaatggtgtt
     6001 ttcatcttct ttcatcttca ccctgtctca cgttacgtta tttttatcct gatgacagaa
     6061 taggtactac aggtattttt actctggttt tacagtcaaa gaaactgagg cacaggcagg
     6121 tttgcccaag gtcacataac tgcttaatag cagggtagac aggtgaagta actggatgtg
     6181 tatcagccat gccctttccc catgcccccg gcactcccca tgacagctct ctgaatccca
     6241 acattttatt cttttgcagg attagaccca agcatataaa ttcctttttc cctctctcca
//
