LOCUS       NM_001377935            6760 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens Rap guanine nucleotide exchange factor 1 (RAPGEF1),
            transcript variant 4, mRNA.
ACCESSION   NM_001377935
VERSION     NM_001377935.1
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Golebiewski,C., Gastaldi,C., Vieu,D.L., Mari,B., Rezzonico,R.,
            Bernerd,F. and Marionnet,C.
  TITLE     Identification and functional validation of SRC and RAPGEF1 as new
            direct targets of miR-203, involved in regulation of epidermal
            homeostasis
  JOURNAL   Sci Rep 13 (1), 14006 (2023)
   PUBMED   37635193
  REMARK    GeneRIF: Identification and functional validation of SRC and
            RAPGEF1 as new direct targets of miR-203, involved in regulation of
            epidermal homeostasis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Pagano,L., Malagrino,F., Nardella,C., Gianni,S. and Toto,A.
  TITLE     Experimental Characterization of the Interaction between the
            N-Terminal SH3 Domain of Crkl and C3G
  JOURNAL   Int J Mol Sci 22 (24), 13174 (2021)
   PUBMED   34947971
  REMARK    GeneRIF: Experimental Characterization of the Interaction between
            the N-Terminal SH3 Domain of Crkl and C3G.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6760)
  AUTHORS   Li,N., Zhou,P., Yang,M., Fang,X., Kramer,N., Mughal,T.A.,
            Abbasi,A.A., Yang,Y., Kaindl,A.M. and Hu,H.
  TITLE     Zebrafish modeling mimics developmental phenotype of patients with
            RAPGEF1 mutation
  JOURNAL   Clin Genet 100 (2), 144-155 (2021)
   PUBMED   33834495
  REMARK    GeneRIF: Zebrafish modeling mimics developmental phenotype of
            patients with RAPGEF1 mutation.
REFERENCE   4  (bases 1 to 6760)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 6760)
  AUTHORS   Sriram,D., Dayma,K., Devi,A.S., Raghawan,A.K., Rawat,S. and
            Radha,V.
  TITLE     Complex formation and reciprocal regulation between GSK3beta and
            C3G
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1868 (5), 118964 (2021)
   PUBMED   33450305
  REMARK    GeneRIF: Complex formation and reciprocal regulation between
            GSK3beta and C3G.
REFERENCE   6  (bases 1 to 6760)
  AUTHORS   Smit,L., van der Horst,G. and Borst,J.
  TITLE     Sos, Vav, and C3G participate in B cell receptor-induced signaling
            pathways and differentially associate with Shc-Grb2, Crk, and Crk-L
            adaptors
  JOURNAL   J Biol Chem 271 (15), 8564-8569 (1996)
   PUBMED   8621483
REFERENCE   7  (bases 1 to 6760)
  AUTHORS   Gotoh,T., Hattori,S., Nakamura,S., Kitayama,H., Noda,M., Takai,Y.,
            Kaibuchi,K., Matsui,H., Hatase,O., Takahashi,H. et al.
  TITLE     Identification of Rap1 as a target for the Crk SH3 domain-binding
            guanine nucleotide-releasing factor C3G
  JOURNAL   Mol Cell Biol 15 (12), 6746-6753 (1995)
   PUBMED   8524240
REFERENCE   8  (bases 1 to 6760)
  AUTHORS   Knudsen,B.S., Feller,S.M. and Hanafusa,H.
  TITLE     Four proline-rich sequences of the guanine-nucleotide exchange
            factor C3G bind with unique specificity to the first Src homology 3
            domain of Crk
  JOURNAL   J Biol Chem 269 (52), 32781-32787 (1994)
   PUBMED   7806500
REFERENCE   9  (bases 1 to 6760)
  AUTHORS   Takai,S., Tanaka,M., Sugimura,H., Yamada,K., Naito,Y., Kino,I. and
            Matsuda,M.
  TITLE     Mapping of the human C3G gene coding a guanine nucleotide releasing
            protein for Ras family to 9q34.3 by fluorescence in situ
            hybridization
  JOURNAL   Hum Genet 94 (5), 549-550 (1994)
   PUBMED   7959692
REFERENCE   10 (bases 1 to 6760)
  AUTHORS   Tanaka,S., Morishita,T., Hashimoto,Y., Hattori,S., Nakamura,S.,
            Shibuya,M., Matuoka,K., Takenawa,T., Kurata,T., Nagashima,K. et al.
  TITLE     C3G, a guanine nucleotide-releasing protein expressed ubiquitously,
            binds to the Src homology 3 domains of CRK and GRB2/ASH proteins
  JOURNAL   Proc Natl Acad Sci U S A 91 (8), 3443-3447 (1994)
   PUBMED   7512734
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL160271.19 and AL160276.21.
            This sequence is a reference standard in the RefSeqGene project.
            
            Summary: This gene encodes a human guanine nucleotide exchange
            factor. It transduces signals from CRK by binding the SH3 domain of
            CRK, and activating several members of the Ras family of GTPases.
            This signaling cascade that may be involved in apoptosis,
            integrin-mediated signal transduction, and cell transformation.
            Several alternatively spliced transcript variants of this gene have
            been described, but the full-length nature of some variants has not
            been determined. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000683357.1/ ENSP00000508246.1
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-307               AL160271.19        56388-56694         c
            308-447             AL160276.21        109584-109723       c
            448-561             AL160276.21        108903-109016       c
            562-740             AL160276.21        102013-102191       c
            741-897             AL160276.21        97409-97565         c
            898-986             AL160276.21        89010-89098         c
            987-1139            AL160276.21        87876-88028         c
            1140-1263           AL160276.21        87323-87446         c
            1264-1447           AL160276.21        86687-86870         c
            1448-1948           AL160276.21        84696-85196         c
            1949-2151           AL160276.21        80570-80772         c
            2152-2307           AL160276.21        77825-77980         c
            2308-2565           AL160276.21        63705-63962         c
            2566-2658           AL160276.21        62735-62827         c
            2659-2747           AL160276.21        60835-60923         c
            2748-2859           AL160276.21        56973-57084         c
            2860-2935           AL160276.21        55072-55147         c
            2936-3020           AL160276.21        50873-50957         c
            3021-3113           AL160276.21        48660-48752         c
            3114-3299           AL160276.21        47575-47760         c
            3300-3384           AL160276.21        46716-46800         c
            3385-3479           AL160276.21        46510-46604         c
            3480-3558           AL160276.21        43292-43370         c
            3559-3660           AL160276.21        43085-43186         c
            3661-3758           AL160276.21        41379-41476         c
            3759-3887           AL160276.21        39037-39165         c
            3888-6760           AL160276.21        35549-38421         c
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.13"
     gene            1..6760
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /note="Rap guanine nucleotide exchange factor 1"
                     /db_xref="GeneID:2889"
                     /db_xref="HGNC:HGNC:4568"
                     /db_xref="MIM:600303"
     exon            1..307
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     CDS             247..4038
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /note="isoform d is encoded by transcript variant 4;
                     guanine nucleotide-releasing factor 2 (specific for crk
                     proto-oncogene); CRK SH3-binding GNRP; Rap guanine
                     nucleotide exchange factor (GEF) 1"
                     /codon_start=1
                     /product="rap guanine nucleotide exchange factor 1 isoform
                     d"
                     /protein_id="NP_001364864.1"
                     /db_xref="CCDS:CCDS94521.1"
                     /db_xref="GeneID:2889"
                     /db_xref="HGNC:HGNC:4568"
                     /db_xref="MIM:600303"
                     /translation="MSGGLGLRRSPEMSGKIEKADSQRSHLSSFTMKLMDKFHSPKIK
                     RTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQ
                     RQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDP
                     RIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKE
                     LVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPD
                     ATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPV
                     GINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCS
                     RNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLP
                     LGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGE
                     DLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNK
                     HMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAP
                     PPALPPKQRQLASCAASSFSSVSHCVQQTKVAFTPEDGSAAQGLSVSVSNSFLSRHGS
                     LPVPSYKSVFRSYSQDFVPHHQASVPPFLPPTSSSSPHFPPAHQSQSSDLAVPTMAGP
                     PPSTVDGPLSASQESSFHGNTVCLPSETSFTDSSENASEEAGEGEYVNLYSSGQSSEE
                     LAPSRGEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSL
                     IDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFIS
                     PEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELV
                     FRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEI
                     AEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEK
                     AQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTL
                     IDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNI
                     LDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEK
                     T"
     exon            308..447
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            448..561
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            562..740
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            741..897
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            898..986
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1139
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1263
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1264..1447
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1948
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            1949..2151
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2152..2307
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2308..2565
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2566..2658
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2659..2747
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2748..2859
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2860..2935
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            2936..3020
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3021..3113
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3114..3299
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3300..3384
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3385..3479
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3480..3558
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3559..3660
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3661..3758
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3759..3887
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     exon            3888..6760
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6735..6740
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /note="hexamer: AATAAA"
     polyA_site      6760
                     /gene="RAPGEF1"
                     /gene_synonym="C3G; GRF2"
                     /note="major polyA site"
ORIGIN      
        1 gtcgctgcgc tgcggccggc tcgcgggccg ggggagcggc ggcgggcgcg cgggaggcgg
       61 agcggcggcg gcgggcgcgg cgggagcggc tgggcccggg cgggctggag cgcggtcgcc
      121 gagcgcaggg ccgcggccgc gcggagcccg ccatgccggc gcgggtctga ggcgtggcga
      181 gccagggccg aggcgagcgg cgggcgcgcc gcccctgtcg ccccgcggcg gcccggcccg
      241 ggcccgatga gcggcggcct cggcctccgg cgcagcccgg aaatgtccgg caagatcgag
      301 aaagcagact ctcagcgttc tcatctctct tccttcacca tgaagctgat ggacaaattc
      361 cactcaccca aaatcaagag aacgccatca aagaagggaa aaccagctga ggtgtccgta
      421 aagattccag agaagcctgt gaacaaagag gcaacagaca gatttctacc agagggctac
      481 cctctcccct tggatctgga gcagcaggca gtagaattta tgtccaccag tgctgtggct
      541 tccaggtctc aaaggcagaa gaacctgagc tggctggagg agaaagagaa ggaagttgtc
      601 agtgccctgc gctactttaa gaccattgtg gacaaaatgg caattgataa gaaggtactg
      661 gagatgcttc cagggtcagc cagcaaggtg ctggaggcca tcttacccct ggtgcagaac
      721 gatcctcgaa ttcagcacag ctcagccctc tcttcctgct atagccgagt gtaccaaagc
      781 ctcgccaacc tcattcgctg gtctgaccaa gtgatgctgg aaggcgtgaa ctcagaagac
      841 aaggagatgg tgacgactgt gaagggggtc atcaaggctg tgctggatgg agtgaaggag
      901 ctggtcaggc tcaccatcga gaagcaggga cgtccgtctc cgacgagccc cgtgaagccc
      961 agttcccctg ccagcaagcc tgatggccca gcagagctcc ccctgacaga ccgcgaggta
     1021 gagatcctaa acaagacgac tgggatgtca cagtcaactg agctcctccc agatgccacg
     1081 gatgaagagg tcgcgccccc caagcctcct ctgcctggca ttcgggtggt tgataatagt
     1141 cctccaccag cattgccacc caagaaaaga cagtcggcgc cgtcccctac ccgagtggct
     1201 gtggtggccc ccatgagccg agccaccagt ggctccagtt tgcctgttgg aatcaatagg
     1261 caggattttg atgttgactg ttacgcacag aggcgactgt caggaggcag ccactcatat
     1321 ggtggagagt cgccccgcct ctccccctgc agcagcatag gcaagctcag caagtcagac
     1381 gagcagctgt cctctctgga cagggacagt gggcagtgct cccggaacac aagctgtgaa
     1441 acactagacc actatgatcc cgactatgaa ttcctccagc aagacctctc taacgcagac
     1501 cagatacctc agcagacggc ctggaacctt agcccgttgc cagagtcttt gggggagtct
     1561 gggtctccat ttcttggccc tcctttccag ctgcctcttg gcggccatcc ccagccagac
     1621 ggacctctgg ccccagggca gcagacagat acgccacctg ctctccccga gaagaagcgc
     1681 aggagcgcag cctcccagac ggcggacggc tctggctgca gggtgtccta cgagcggcat
     1741 ccctcgcagt atgacaacat ctctggggag gacctgcaga gcacagcccc gatcccatcc
     1801 gtcccctacg cgccctttgc tgctattctg ccctttcagc atggaggttc ctcagcccct
     1861 gtcgaatttg tgggtgattt tactgctcct gagtcaaccg gtgacccaga aaaaccacct
     1921 cctctaccag agaagaaaaa caaacacatg ctggcctaca tgcagttgct ggaggactac
     1981 tcggagccgc agccctctat gttctaccag acgccacaga acgagcacat ctaccagcag
     2041 aagaacaagc tcctcatgga ggtatacggc ttcagcgact ccttcagtgg ggtggactcc
     2101 gtgcaggagc tggccccgcc gcccgcccta ccccccaagc agcggcagct ggcctcctgt
     2161 gctgcttctt ccttctcctc tgtctcccac tgtgtccagc aaactaaagt ggccttcacc
     2221 cccgaggacg gcagtgccgc tcagggcctc agtgtgtccg tctctaactc ctttctcagc
     2281 cggcacggca gcttgcccgt gccctcgtat aagtctgtgt ttaggtccta ctcccaggat
     2341 ttcgtgcctc accaccaagc ttccgttccg cctttccttc cgcctacctc ctcttcctct
     2401 ccacatttcc cacctgccca ccagtctcag agctctgact tagccgtgcc aaccatggcc
     2461 ggtccacctc ccagcaccgt ggacgggcct ctctcggctt ctcaggagag cagctttcat
     2521 gggaatactg tctgccttcc ttccgaaacc tctttcactg actcgagtga aaacgccagt
     2581 gaggaagctg gtgagggtga atatgtcaat ctgtattcct ctggccagag cagcgaggag
     2641 ctggctccct ctcgaggaga gccaccggct gggaaagacg gacatcccag agatccctca
     2701 gcggtcagcg gcgtccctgg gaaggacagc agagacggca gtgagagggc cccaaagtca
     2761 ccagatgctc tggagtcggc tcagtcggag gaggaagtgg acgagctgtc cctcattgac
     2821 cacaacgaaa ttatgtccag gctgacgctc aagcaggagg gtgatgacgg gccggacgtc
     2881 cgcggaggat ctggggacat cttactggtc catgctactg agactgacag gaaagatttg
     2941 gtgttgtact gcgaggcatt cctgaccacc tacaggacct tcatctcccc agaggagctc
     3001 atcaagaagc tgcagtacag atatgagaaa ttctctccct ttgccgacac attcaagaag
     3061 cgcgtcagca agaacacgtt cttcgtgctg gtacgggtgg tggatgagct ctgcctggtg
     3121 gagttgacag aagagatcct gaagctgctg atggaactgg tcttccgcct ggtgtgcaat
     3181 ggggagctga gcctggcccg tgtgctccgg aagaacatcc tggacaaggt ggaccagaag
     3241 aagctactca ggtgtgccac ctccagccag cccctggcag cccggggggt agcagccagg
     3301 ccggggacct tgcacgactt tcacagccat gagatagcgg agcagctaac gctgctggat
     3361 gctgagctct tctataaaat agagattcct gaggttttgc tttgggcaaa agagcagaat
     3421 gaggagaaga gccccaactt gacccagttc acggagcact tcaacaacat gtcctactgg
     3481 gtccggtcca taatcatgtt acaggaaaag gcccaggaca gggaacggct gctcttgaag
     3541 ttcatcaaga tcatgaagca cttgcggaag ctgaataact tcaactccta cttggccatc
     3601 ctctctgccc tggactcggc gcccatccgc aggctggagt ggcagaagca gacttcagag
     3661 ggcctggccg agtactgcac actgatcgac agctcgtcct ccttccgagc ctaccgggcc
     3721 gccctctcgg aggtggaacc gccgtgcatc ccgtacctgg ggctgatcct gcaggacctg
     3781 accttcgttc acctgggaaa cccagactac atcgacggga aagtgaactt ctccaagcgg
     3841 tggcagcagt tcaacatcct cgacagcatg cgctgcttcc agcaggcgca ctatgacatg
     3901 cggaggaacg acgacattat aaacttcttc aatgacttca gtgaccacct ggctgaggag
     3961 gccctatggg aactgtctct gaaaattaaa cccaggaaca taacaaggag aaaaacagac
     4021 cgggaagaga agacctagga gcagacgccg ggatccagga gaatgctcga ggggcgcaga
     4081 gggcagctcc cagaccggag aggaccttgg acctgttagg cgcatggcag gagtcccggc
     4141 ctcggagcca tgaggctggc cagccctcag cggggccggg cgggagctgg agcctgccag
     4201 ccgcttcctg cctccttcct ctgtgggagc agacccgtgg gcctcagggc agccagcagg
     4261 caggtcttgt tgccaattta caaaccggtg gttttctggt ttggttttgt tttctgcttt
     4321 tacttccatc tctcccctct tgaccttcca cccactcccc tccagggaga gagcagcaga
     4381 gacctcatca gcagaccaag gaagtggtgg gtgctccccc tccctaagct ccagggtccc
     4441 tgaatcttct gaaatctcaa atgagtggag gcctcctggg gtggcctgtc ctgcaggggc
     4501 cctggaatgg gggcaagcag ctgggtgggc agaatgcaga gtagactcgg gggaggatcc
     4561 tttcactttc cgcttcccct tctgatgcat ggaggatggt gtgagctttt cagcaggccc
     4621 ggaaaggtac gcaggtgacg ccttagcagc cccgcagctg gtgctctgcc ccgcggtact
     4681 ggcgccatca gggcctccct tgcccgcctg agagcagcag cagtctctgt catcccgtcg
     4741 ccccttaccc cccaccccag gccactgggc ccctcccaca ccacctgggg agctgagaag
     4801 aggaggctgg agtaagggag gacttgatca tccaagaaat actttttatt gctgggagtc
     4861 ttctgaacct caccaaactg aggccagagc tgagctcctg ggggagttaa ttcagagggg
     4921 agaggccagc acctccctcc tccatcgctc gctgtgtgcc ttaaactcca tctcatgtcc
     4981 ctccccatcc cctggctttc cctccctcct tgccccatcc tgggccagcc agcagggctc
     5041 ctcctctggc tcttcagacc tttcagccag tgctgtcagt gcccctggga gggaagggca
     5101 tccctgaggc acccgaatgg tccctcaggg tgcagggagg cagaagcctg gccacagagg
     5161 agcctcctaa ggcagcagct gcagcaagcg caccctctcc ccactctccc cacgccagag
     5221 cggcttccag agcagatgct gtttccatcc tcctcgtcaa aaccattctc gctgctgagc
     5281 ttgacaatct gggcaaggct tgtggggcgc ttgacaaaca gaatctgccc tgtgccgcct
     5341 ggttccgtgg cctccagcat gagcctgcag gcagggcgct gcgggaaccc agttgtgctg
     5401 ccccagccca tgcctccggg tctgctgtgc atgaatgagt gctcacttgt cccgggttta
     5461 ggacgtggtc aagtgaacag cagggtctaa ctgtgcttac ttagcccagt tcaaacagaa
     5521 caaaggaaaa atatagaaag caacatctgt tgatcattta ggtttttttt taaaccacca
     5581 tgtcactttg agtccttcat gggtttttga acagcattta tcaagaagaa aatgtgggct
     5641 ttttcccctc tcccgtgttt tgtttgtcct gtagatagag ggaggaaagc cgtgcagtgg
     5701 caggcgggac cccctctggt ggcgggaccc cctcttgcgg tggtcttgcg gggccagccg
     5761 ggacctgtca ctttattatt taaggagtgt gtgtgtagag tcgctggctt attaacagta
     5821 ttgtgtgtgg gttgggtttt tagtttgttc cttctttttg aagtcccttc atttcaatcc
     5881 ttgactctct ctccccttcc cttgcccagc tctgttgaat gctgctgtgc gcgtgtgagg
     5941 gccgctctgc acacagggcc cttgggttgt gtgaactgaa attctccctg tatttgtgag
     6001 actcgcagga gtccccatct gtagcacagg caatgccagt gccatgctgc agcctcagaa
     6061 accaggcctc tcactccagc agcaggcaga accgtgtctg tggtcgggtg ctgtccacag
     6121 ctctgtctgc cttgttcttg ggcttgagct ggatagaggt ggggtctctt caccttccct
     6181 gaattcagaa cagaccctgt gcctggcccc agtgtgccca ggcaattccc caggccctca
     6241 ctgggagccc ttggtgttct gagcagcagg gcccaggcag cacatgagca gtgcccaggg
     6301 gctccctgcg tgaggacggc aaggtgcgat gtatgtctaa cttattgatg gcaggcagcc
     6361 ccctgtgccc cctaagcctg gccctggtta ttgctgagct ctgtgctcag tgctgcggcc
     6421 tggccgtggc tcgtctgttc ctttgggggg cccgggcggg ttgtgggaat cagtcttcac
     6481 agacagacgt gagccaggcg gaggactcgt tccttgcaga ggtcagtcct cacctgcagg
     6541 tgtcggggtg tgggggggca aggaggggca ggcacacacc atgtctgacc tgaacccgat
     6601 tctggggagc atcttcccgc tccggcccca cgacctccac agggttacat tgtaatatat
     6661 atgccccagc taacctgtct gatggtggca tcttcctgca gacatttcaa acatgtaact
     6721 tttatatgaa aaaaaataaa cacagatgaa agctgcccaa
//
