LOCUS       NM_021033               5540 bp    mRNA    linear   PRI 11-JUN-2024
DEFINITION  Homo sapiens RAP2A, member of RAS oncogene family (RAP2A), mRNA.
ACCESSION   NM_021033
VERSION     NM_021033.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Wu,C., Cai,X., Wang,Y., Rodriguez,C.D., Zoaldi,G., Herrmann,L.,
            Huang,C.Y., Wang,X., Sanghvi,V.R., Lu,R.O. and Meng,Z.
  TITLE     Interplay of RAP2 GTPase and the cytoskeleton in Hippo pathway
            regulation
  JOURNAL   J Biol Chem 300 (5), 107257 (2024)
   PUBMED   38574891
  REMARK    GeneRIF: Interplay of RAP2 GTPase and the cytoskeleton in Hippo
            pathway regulation.
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Duncan,E.D., Han,K.J., Trout,M.A. and Prekeris,R.
  TITLE     Ubiquitylation by Rab40b/Cul5 regulates Rap2 localization and
            activity during cell migration
  JOURNAL   J Cell Biol 221 (4) (2022)
   PUBMED   35293963
  REMARK    GeneRIF: Ubiquitylation by Rab40b/Cul5 regulates Rap2 localization
            and activity during cell migration.
REFERENCE   3  (bases 1 to 5540)
  AUTHORS   Jin,K., Liu,C., Cheng,H., Fei,Q., Huang,Q., Xiao,Z., Yu,X. and
            Wu,W.
  TITLE     TGF-beta1-induced RAP2 regulates invasion in pancreatic cancer
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 54 (3), 361-369 (2022)
   PUBMED   35538031
  REMARK    GeneRIF: TGF-beta1-induced RAP2 regulates invasion in pancreatic
            cancer.
REFERENCE   4  (bases 1 to 5540)
  AUTHORS   Shi,S., Li,J., Li,E., Guo,W., He,Y., Wang,J., Zhang,Y., Yue,L. and
            Wei,L.
  TITLE     Simulated Microgravity Increases the Permeability of HUVEC
            Monolayer through Up-Regulation of Rap1GAP and Decreased Rap2
            Activation
  JOURNAL   Int J Mol Sci 23 (2), 630 (2022)
   PUBMED   35054818
  REMARK    GeneRIF: Simulated Microgravity Increases the Permeability of HUVEC
            Monolayer through Up-Regulation of Rap1GAP and Decreased Rap2
            Activation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5540)
  AUTHORS   Lian,Y., Jiang,D. and Sun,J.
  TITLE     Tumor suppressive role of miR-33a-5p in pancreatic ductal
            adenocarcinoma cells by directly targeting RAP2A
  JOURNAL   Cell Mol Biol Lett 26 (1), 24 (2021)
   PUBMED   34090323
  REMARK    GeneRIF: Tumor suppressive role of miR-33a-5p in pancreatic ductal
            adenocarcinoma cells by directly targeting RAP2A.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5540)
  AUTHORS   Janoueix-Lerosey,I., Pasheva,E., de Tand,M.F., Tavitian,A. and de
            Gunzburg,J.
  TITLE     Identification of a specific effector of the small GTP-binding
            protein Rap2
  JOURNAL   Eur J Biochem 252 (2), 290-298 (1998)
   PUBMED   9523700
REFERENCE   7  (bases 1 to 5540)
  AUTHORS   Cherfils,J., Menetrey,J., Le Bras,G., Janoueix-Lerosey,I., de
            Gunzburg,J., Garel,J.R. and Auzat,I.
  TITLE     Crystal structures of the small G protein Rap2A in complex with its
            substrate GTP, with GDP and with GTPgammaS
  JOURNAL   EMBO J 16 (18), 5582-5591 (1997)
   PUBMED   9312017
REFERENCE   8  (bases 1 to 5540)
  AUTHORS   Farrell,F.X., Yamamoto,K. and Lapetina,E.G.
  TITLE     Prenyl group identification of rap2 proteins: a ras superfamily
            member other than ras that is farnesylated
  JOURNAL   Biochem J 289 (Pt 2) (Pt 2), 349-355 (1993)
   PUBMED   8424780
REFERENCE   9  (bases 1 to 5540)
  AUTHORS   Lerosey,I., Chardin,P., de Gunzburg,J. and Tavitian,A.
  TITLE     The product of the rap2 gene, member of the ras superfamily.
            Biochemical characterization and site-directed mutagenesis
  JOURNAL   J Biol Chem 266 (7), 4315-4321 (1991)
   PUBMED   1900290
REFERENCE   10 (bases 1 to 5540)
  AUTHORS   Pizon,V., Chardin,P., Lerosey,I., Olofsson,B. and Tavitian,A.
  TITLE     Human cDNAs rap1 and rap2 homologous to the Drosophila gene Dras3
            encode proteins closely related to ras in the 'effector' region
  JOURNAL   Oncogene 3 (2), 201-204 (1988)
   PUBMED   3045729
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL442067.16, DB480985.1, BC041333.2, DA493957.1, BC022356.1,
            BC047495.1 and CB215818.1.
            
            On Nov 22, 2018 this sequence version replaced NM_021033.6.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC041333.2, SRR1803614.209087.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000245304.5/ ENSP00000245304.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-52                AL442067.16        80577-80628
            53-391              DB480985.1         56-394
            392-1321            BC041333.2         341-1270
            1322-1682           DA493957.1         219-579
            1683-2041           BC041333.2         1632-1990
            2042-2117           BC022356.1         875-950
            2118-2635           BC041333.2         2067-2584
            2636-3557           BC041333.2         2587-3508
            3558-4100           BC047495.1         630-1172
            4101-4402           BC041333.2         4052-4353
            4403-4410           CB215818.1         263-270
            4411-5540           AL442067.16        114407-115536
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="13"
                     /map="13q32.1"
     gene            1..5540
                     /gene="RAP2A"
                     /gene_synonym="K-REV; KREV; RAP2; RbBP-30"
                     /note="RAP2A, member of RAS oncogene family"
                     /db_xref="GeneID:5911"
                     /db_xref="HGNC:HGNC:9861"
                     /db_xref="MIM:179540"
     exon            1..616
                     /gene="RAP2A"
                     /gene_synonym="K-REV; KREV; RAP2; RbBP-30"
                     /inference="alignment:Splign:2.1.0"
     CDS             303..854
                     /gene="RAP2A"
                     /gene_synonym="K-REV; KREV; RAP2; RbBP-30"
                     /EC_number="3.6.5.2"
                     /note="RAP2, member of RAS oncogene family (K-rev)"
                     /codon_start=1
                     /product="ras-related protein Rap-2a"
                     /protein_id="NP_066361.1"
                     /db_xref="CCDS:CCDS9485.1"
                     /db_xref="GeneID:5911"
                     /db_xref="HGNC:HGNC:9861"
                     /db_xref="MIM:179540"
                     /translation="MREYKVVVLGSGGVGKSALTVQFVTGTFIEKYDPTIEDFYRKEI
                     EVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIIR
                     VKRYEKVPVILVGNKVDLESEREVSSSEGRALAEEWGCPFMETSAKSKTMVDELFAEI
                     VRQMNYAAQPDKDDPCCSACNIQ"
     exon            617..5540
                     /gene="RAP2A"
                     /gene_synonym="K-REV; KREV; RAP2; RbBP-30"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4384..4389
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAP2A"
                     /gene_synonym="K-REV; KREV; RAP2; RbBP-30"
                     /note="hexamer: AATAAA"
     polyA_site      4403
                     /gene="RAP2A"
                     /gene_synonym="K-REV; KREV; RAP2; RbBP-30"
                     /note="major polyA site"
     regulatory      4463..4468
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAP2A"
                     /gene_synonym="K-REV; KREV; RAP2; RbBP-30"
                     /note="hexamer: ATTAAA"
     polyA_site      4485
                     /gene="RAP2A"
                     /gene_synonym="K-REV; KREV; RAP2; RbBP-30"
     regulatory      5441..5446
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAP2A"
                     /gene_synonym="K-REV; KREV; RAP2; RbBP-30"
                     /note="hexamer: AATAAA"
     polyA_site      5471
                     /gene="RAP2A"
                     /gene_synonym="K-REV; KREV; RAP2; RbBP-30"
     regulatory      5521..5526
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAP2A"
                     /gene_synonym="K-REV; KREV; RAP2; RbBP-30"
                     /note="hexamer: AATAAA"
     polyA_site      5540
                     /gene="RAP2A"
                     /gene_synonym="K-REV; KREV; RAP2; RbBP-30"
ORIGIN      
        1 actgcgggcc cgcggctcgg gcggcaccag cggctcccgg tctctctctc tgctcgccct
       61 cagtcccacc cggtgcctac ggggccgcat cgccgcccgg ctcggcccgg cccatgccca
      121 gggccgctgc tccctcagtt gccgccgccg ccgccggcgc ccagggggcc gccgcggctg
      181 tgaggtgggg gcggcagcgg gaggcggcgg ggcgggccgc cggggccggg gctgggggcg
      241 cagcgcggcc ggcgtaggtc tatgtcgcgg gcggcggcgg cggcggcggc cgcggaggga
      301 cgatgcgcga gtacaaagtg gtggtgctgg gctcgggcgg ggtaggcaaa tccgccctga
      361 ccgtgcagtt cgtgaccggc accttcatcg agaaatacga ccccaccatc gaggacttct
      421 accgcaagga gatcgaggtg gattcgtcgc cgtcggtgct ggagatcctg gacacggcgg
      481 gcaccgagca gttcgcgtcc atgcgggacc tgtacatcaa gaacggccag ggcttcatcc
      541 tcgtctacag cctcgtcaac cagcagagct tccaggacat caagcccatg cgggaccaga
      601 tcatccgcgt gaagcggtat gagaaagtgc cagtcatctt ggttgggaac aaagtggacc
      661 tggaaagtga gagagaagta tcgtccagcg aaggcagagc ccttgctgaa gagtggggct
      721 gcccctttat ggaaacttcc gctaagagta aaacaatggt ggacgaactc tttgcagaaa
      781 ttgtgaggca gatgaactat gctgctcagc ctgacaaaga tgacccatgc tgttctgcat
      841 gtaacataca atagcatcca aatatggctg tcctggatgg gatttgccca atgtcgtagg
      901 tgatagaaaa ctcgcctact ccactgcaga acttgcagaa tgcgtggtgt taatctacag
      961 agaactgcag cccttattca gaattgagca gtgattgtca gttgatatct ctgagtaaca
     1021 tttgggttca gtaactacag ttttcaccat ttgtcctcag tctcctttat gcatctgcaa
     1081 ctttaaggca tagtccatcg atctacaggg tgatctcatt tgaaagctat gatggcattg
     1141 tcgctgagtt gacagaagca actattgtac aaattaaaaa taaccataag ggagaaacca
     1201 gaagaattcc tctgaccact tacaattaaa atattttgtc ttctttaaaa aaataagtaa
     1261 aggagaaata ttttcctaca agagtactga atttcaggaa atgggataga gcttctaacc
     1321 aatgtattcc gtcaagtaag ataataacag ctgacctgcc aacagcatta cagggagatt
     1381 ctttgctcag ctaacacatt tctgtttttc aaaattgatg cttaattgta gctgttattc
     1441 taatttgtga catggaacta actcatgctt caatccttga tagagcaaaa ctcagaacag
     1501 gttatgtaaa aatatagtct ggctttagaa tttgttaatt cacctgcttt gccacagaaa
     1561 atggaggctt tcacaagggt ttgatcagaa ttaaaacccc aaacccgccc tctgttaggg
     1621 gtggtcttta taactttact gaacaagagg gaagcaactg tgatgggaag agattaccta
     1681 gccttactaa caagaagcat tctaatagat aaactagtac catcatgtaa ggaagatgaa
     1741 gccatgttgc atccacatgt tcccgtttgc agaaacctca caggacagta cgaatatctg
     1801 gcatttttaa gttcattaaa gcagcaaatt ccttctcgcc tttaagggct atggttgtag
     1861 tgtgacccgt ggctaacctg ctttcaaaat caagtatttg ctgtagcaga gctttattgc
     1921 aggcatttta aaaattgaat aaccatgtga aataatttgg gcttaaaagt gaacataaat
     1981 tatagagtgg aaggtaaggg acaaaagcct tttaccttta attttcctgg agaattatat
     2041 aaaggttttc ttagaacaat tttgccctga ttactgaagc gtcaaataaa gctggagtga
     2101 aaattttgtt ttgaaaaggt gctcaagctc tgacattttt ggttttcata gtcgtgaagt
     2161 atttatcatt tgcatgacta atggcacagg aaaaacctat tcataagttt tacaatcaag
     2221 tatagagggg tcttcagcta acttagttat acataaatgc tgaaaagatt atcttgagct
     2281 gggccttggg agaagaattt ggccttcaga actgcaatat cactgagcct gcgatctata
     2341 caccacccca cattttgttg gtttcacagt cttgtgcaag taacctctgg tcttacgtgt
     2401 gtaactgaga gaagagtgtg tgtttgtgtg tgcatgtgtg tttattgttc ctaagaattt
     2461 ggcacaagtc agagataatt gcctatacta agaatctata ctgcagaata tagtgtatca
     2521 aaaacttttt tcttttaaat tattaaagtg tcttttatac ttttatgaaa tcattggtag
     2581 ccccccaagt gtttaataac tggcattaag cttagagggt gaaaaaaaaa aaaaagattg
     2641 atagtatttt tcataatgaa aaaactgaag agatacgtga atgaaaccag gccatagctg
     2701 tcataaatct ttgacttttg aatatttaca ttcttcagta taatttttta taatcctcaa
     2761 ttatgaacca ccttgtttat aggacaaaaa aatttaacca attttattga aacgaatttc
     2821 actgtgtaaa agttggtttg attcaaacat gtagagaagt tgtagattca agatatatga
     2881 tttctctatg gaaataaaaa tatttgttag tgaattggtt gagttttgat tcctctaact
     2941 tctcagaatg attctttaga attctataat tcatagcaat ttttgacaag taagattgca
     3001 aaatagaaat atctataaag attccacagt ttgacattat ggcttgctat gcagatgtga
     3061 aaataggtta aataatatga aagatatggc agaatgtaaa gtggaaaaga tgacctaaaa
     3121 ttttgagttg tattaatagt taaaaacatt tgtgtcagat gacagggtgg gcttttactg
     3181 tcaagacatg aataagaact gatctggctg cctgatgagt gtttccacgc agccctgcat
     3241 atttagtgac caaggcatca aggacatccc gaaactggaa attcatatcc atctggtatg
     3301 aatatataac tcagctggca aatgaatgtg tttgttgaga tattacagta ataaaacact
     3361 taagaacagg aagattacat ttgttggcat acgaaacctt agtggctaca gaagaaagtt
     3421 gaccttgtgt cactatttat tttatgccct gatcagacta gcaacttaga taagtgaaag
     3481 tttttctaac atgccttaaa aatattatgg tttgatccaa agacccactt tttctttagc
     3541 tcttgtgata agattttctt ttttttactt ttatacaaag gcagcatctt tgaatttttt
     3601 tttcttttga tgttgcaact tttgggttct tttaaactgt gatagtgatg gtaactgatg
     3661 cctttcattt tgttcaactt atacaaaaca agccagcatc tgatcaaaag tattacataa
     3721 aatattttct taaactattg aaaggtgctt tgatgatttt ctcctttggt ttgtagaatt
     3781 aggactgaac ttttgactca aattgctaca gttgccatca cctttctgtg gtaatactac
     3841 tgatatttgc ttttctatat aaagaaatgt tgcctaaggc tgtctggtat ttcttttcaa
     3901 gggttttcca gtatgaatgt taatgttgtc agtgtatgta tgaatatgaa agtgctttgt
     3961 tttgtttgtt gctgtttttt gtttatgtgt gtgtttttaa tttttttgtt cttatcagca
     4021 gtcttgtgtt agcactgggt aagctttaat tgtcccttag ccaatcaaac attaaggact
     4081 atggaggtct tttttttttt atttaacatg tcattgttca tctattaaat cttgatcagg
     4141 gtttcaagaa tgactgcagt gggttttgga aacagactta tcattattga tttgaggttt
     4201 cccagagata tagttcacag ttaattgttg cgctctaata caactgacca tttaaaattg
     4261 aacaagttta ttgttttgta acaatgtcag ttgttaaacc ttgacatttc aattaaaaca
     4321 tgaattgtag ttataactca atgcaaattc aacagttgta tttggagtta aattatttta
     4381 acaaataaat ttatttaatg aaactctgga tttgctttgt tttgtacatg ctcatgagag
     4441 aaatgtatta tatacaaaaa caattaaaaa ttgccatata atgaatttag tactcttcat
     4501 acaaaaatat tttagctgat ttgagagttc atcatataaa aaggcaggta ggtagataat
     4561 tttcatagtc tttaaaaata aaaataacat gtaagagact gtaaaatcag ttattataaa
     4621 tataaactta tctctacaaa tgattttata ttttgaatgg tagctggtta atttaaagcc
     4681 tgatgctaag gttaacagaa ttgttcaaat aattaacatt aattagcaat atcactaaaa
     4741 tgattcagag gccttctagt gatggcttga agccgtcttt ctgttttcag gtcatgtgga
     4801 tccgaaggtg ccagaagctg aaggttgccg ccaggctgta gggcctgctt gttctatatg
     4861 gggccctatg aaagttgaca gatgctgtta attgtataca gccttcagct gctctgataa
     4921 ggagcccatt catttcctaa gccaatttaa actatgcctt ttggttatgt tgccttcaaa
     4981 ctctgttgaa atcctctggc agcattccac cagttgagaa gcttcattgt tttagattat
     5041 aagaataatt tgaatgaata taacagaggc actgtagata gaatcccaga tatttaaagg
     5101 aaaacatgcc tatattttag gaatatcttc tgaagaactt cataatttta tttgaagtgt
     5161 ttgtaaattt tttaataaat tatttaaatg tatcaatagt ggatagttgg aagaacgtct
     5221 ttgaggctaa aaagtagtac tctgaaattc tctcaagtta tattatttgt ctgttagagg
     5281 tccacgaatg aaaatatatg tagctaaagg agtaaggtgc tgcccaattt ggaacccaac
     5341 tgacctctag gatctatgaa gcagagaata ttaccacaaa cttctgaaag aagctgttga
     5401 cctcgtttct taatttaaaa tatgttgcca cataaatact aataaaacat agttgctctt
     5461 catctcttaa atggatcatt ccaactggtt ttacatcgtt gaatattatc tagtattttt
     5521 aataaatatt ttcataaata
//
