LOCUS       NM_001100427            3747 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens Rap1 GTPase-GDP dissociation stimulator 1 (RAP1GDS1),
            transcript variant 3, mRNA.
ACCESSION   NM_001100427
VERSION     NM_001100427.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Zhou,Y., Liu,Y., Gupta,S., Paramo,M.I., Hou,Y., Mao,C., Luo,Y.,
            Judd,J., Wierbowski,S., Bertolotti,M., Nerkar,M., Jehi,L.,
            Drayman,N., Nicolaescu,V., Gula,H., Tay,S., Randall,G., Wang,P.,
            Lis,J.T., Feschotte,C., Erzurum,S.C., Cheng,F. and Yu,H.
  TITLE     A comprehensive SARS-CoV-2-human protein-protein interactome
            reveals COVID-19 pathobiology and potential host therapeutic
            targets
  JOURNAL   Nat Biotechnol 41 (1), 128-139 (2023)
   PUBMED   36217030
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 3747)
  AUTHORS   Zheng,Y.X., Wang,L., Kong,W.S., Chen,H., Wang,X.N., Meng,Q.,
            Zhang,H.N., Guo,S.J., Jiang,H.W. and Tao,S.C.
  TITLE     Nsp2 has the potential to be a drug target revealed by global
            identification of SARS-CoV-2 Nsp2-interacting proteins
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 53 (9), 1134-1141 (2021)
   PUBMED   34159380
REFERENCE   4  (bases 1 to 3747)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 3747)
  AUTHORS   Sato,T., Mukai,S., Ikeda,H., Mishiro-Sato,E., Akao,K.,
            Kobayashi,T., Hino,O., Shimono,W., Shibagaki,Y., Hattori,S. and
            Sekido,Y.
  TITLE     Silencing of SmgGDS, a Novel mTORC1 Inducer That Binds to RHEBs,
            Inhibits Malignant Mesothelioma Cell Proliferation
  JOURNAL   Mol Cancer Res 19 (5), 921-931 (2021)
   PUBMED   33574130
  REMARK    GeneRIF: Silencing of SmgGDS, a Novel mTORC1 Inducer That Binds to
            RHEBs, Inhibits Malignant Mesothelioma Cell Proliferation.
REFERENCE   6  (bases 1 to 3747)
  AUTHORS   Lanning,C.C., Ruiz-Velasco,R. and Williams,C.L.
  TITLE     Novel mechanism of the co-regulation of nuclear transport of SmgGDS
            and Rac1
  JOURNAL   J Biol Chem 278 (14), 12495-12506 (2003)
   PUBMED   12551911
REFERENCE   7  (bases 1 to 3747)
  AUTHORS   Vikis,H.G., Stewart,S. and Guan,K.L.
  TITLE     SmgGDS displays differential binding and exchange activity towards
            different Ras isoforms
  JOURNAL   Oncogene 21 (15), 2425-2432 (2002)
   PUBMED   11948427
REFERENCE   8  (bases 1 to 3747)
  AUTHORS   Shimizu,K., Kawabe,H., Minami,S., Honda,T., Takaishi,K.,
            Shirataki,H. and Takai,Y.
  TITLE     SMAP, an Smg GDS-associating protein having arm repeats and
            phosphorylated by Src tyrosine kinase
  JOURNAL   J Biol Chem 271 (43), 27013-27017 (1996)
   PUBMED   8900189
REFERENCE   9  (bases 1 to 3747)
  AUTHORS   Riess,O., Epplen,C., Siedlaczck,I. and Epplen,J.T.
  TITLE     Chromosomal assignment of the human smg GDP dissociation stimulator
            gene to human chromosome 4q21-q25
  JOURNAL   Hum Genet 92 (6), 629-630 (1993)
   PUBMED   8262526
REFERENCE   10 (bases 1 to 3747)
  AUTHORS   Kikuchi,A., Kaibuchi,K., Hori,Y., Nonaka,H., Sakoda,T.,
            Kawamura,M., Mizuno,T. and Takai,Y.
  TITLE     Molecular cloning of the human cDNA for a stimulatory GDP/GTP
            exchange protein for c-Ki-ras p21 and smg p21
  JOURNAL   Oncogene 7 (2), 289-293 (1992)
   PUBMED   1549351
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DC367998.1, AK055032.1, BC098269.1, AC058823.4 and AI800264.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001100427.1.
            
            Summary: The smg GDP dissociation stimulator (smgGDS) protein is a
            stimulatory GDP/GTP exchange protein with GTPase activity (Riess et
            al., 1993 [PubMed 8262526]).[supplied by OMIM, Feb 2010].
            
            Transcript Variant: This variant (3) uses an alternate in-frame
            splice site in the 5' coding region, compared to variant 1,
            resulting in a shorter protein (isoform 3), compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.207510.1,
                                           SRR1803612.160916.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000408927.8/ ENSP00000386153.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-69                DC367998.1         9-77
            70-378              AK055032.1         1-309
            379-891             BC098269.1         248-760
            892-1334            AK055032.1         823-1265
            1335-2137           BC098269.1         1204-2006
            2138-3546           AC058823.4         109390-110798
            3547-3747           AI800264.1         13-213              c
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q23"
     gene            1..3747
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /note="Rap1 GTPase-GDP dissociation stimulator 1"
                     /db_xref="GeneID:5910"
                     /db_xref="HGNC:HGNC:9859"
                     /db_xref="MIM:179502"
     exon            1..186
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /inference="alignment:Splign:2.1.0"
     CDS             183..2006
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /note="isoform 3 is encoded by transcript variant 3; SMG
                     GDS protein; exchange factor smgGDS; SMG P21 stimulatory
                     GDP/GTP exchange protein; RAP1, GTP-GDP dissociation
                     stimulator 1"
                     /codon_start=1
                     /product="rap1 GTPase-GDP dissociation stimulator 1
                     isoform 3"
                     /protein_id="NP_001093897.1"
                     /db_xref="CCDS:CCDS43253.1"
                     /db_xref="GeneID:5910"
                     /db_xref="HGNC:HGNC:9859"
                     /db_xref="MIM:179502"
                     /translation="MDNLSDTLKKLKITAVDKTEDSLEGCLDCLLQALAQNNTETSEK
                     IQASGILQLFASLLTPQSSCKAKVANIIAEVAKNEFMRIPCVDAGLISPLVQLLNSKD
                     QEVLLQTGRALGNICYDSHEGRSAVDQAGGAQIVIDHLRSLCSITDPANEKLLTVFCG
                     MLMNYSNENDSLQAQLINMGVIPTLVKLLGIHCQNAALTEMCLVAFGNLAELESSKEQ
                     FASTNIAEELVKLFKKQIEHDKREMIFEVLAPLAENDAIKLQLVEAGLVECLLEIVQQ
                     KVDSDKEDDITELKTGSDLMVLLLLGDESMQKLFEGGKGSVFQRVLSWIPSNNHQLQL
                     AGALAIANFARNDANCIHMVDNGIVEKLMDLLDRHVEDGNVTVQHAALSALRNLAIPV
                     INKAKMLSAGVTEAVLKFLKSEMPPVQFKLLGTLRMLIDAQAEAAEQLGKNVKLVERL
                     VEWCEAKDHAGVMGESNRLLSALIRHSKSKDVIKTIVQSGGIKHLVTMATSEHVIMQN
                     EALVALALIAALELGTAEKDLESAKLVQILHRLLADERSAPEIKYNSMVLICALMGSE
                     CLHKEVQDLAFLDVVSKLRSHENKSVAQQASLTEQRLTVES"
     exon            187..294
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /inference="alignment:Splign:2.1.0"
     exon            295..417
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /inference="alignment:Splign:2.1.0"
     exon            418..543
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /inference="alignment:Splign:2.1.0"
     exon            544..690
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /inference="alignment:Splign:2.1.0"
     exon            691..819
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /inference="alignment:Splign:2.1.0"
     exon            820..945
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /inference="alignment:Splign:2.1.0"
     exon            946..1089
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1090..1221
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1222..1356
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..1482
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1483..1622
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1623..1749
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1750..1878
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..3747
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2527..2532
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /note="hexamer: AATAAA"
     polyA_site      2555
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /note="major polyA site"
     regulatory      3727..3732
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
                     /note="hexamer: AATAAA"
     polyA_site      3747
                     /gene="RAP1GDS1"
                     /gene_synonym="GDS1; SmgGDS"
ORIGIN      
        1 gctcgtcccc gccgcggccg cgccgcctgc agcagcacca gctgctcctc cccggcggcc
       61 gccccccgcg ggtccctccc tggctgcggg agagacggag gtagagggag gacacagagc
      121 cgcgccgccc gcaccacaga ccttcgcctc gccccgccgg ttcctcaccc tcggggagca
      181 acatggataa tctcagtgat accttgaaga agctgaagat aacagctgtt gacaagactg
      241 aggatagttt agaaggatgc ttggattgtc tgcttcaagc cctggctcaa aataatacgg
      301 aaacaagtga aaaaatccaa gcaagtggaa tacttcagct gtttgcaagt ctgttgactc
      361 cacagtcttc ctgcaaagcc aaagtagcta acatcatagc agaagtagcc aaaaatgagt
      421 ttatgcgaat tccatgtgtg gatgctggat tgatttcacc actggtgcag ctgctaaata
      481 gcaaagacca ggaagtgctg cttcaaacgg gcagggctct aggaaacata tgttacgata
      541 gccatgaggg cagaagtgca gttgaccaag caggtggtgc acagattgta attgaccatt
      601 taaggtcact gtgcagtata acagatcccg ccaatgagaa gctcttgact gtcttttgtg
      661 gcatgctgat gaactatagc aatgagaatg attcgcttca agctcagctt atcaatatgg
      721 gtgttattcc taccttagtg aaattactgg gcatccactg ccaaaatgca gctcttacag
      781 aaatgtgtct tgttgcattt ggtaatttag cagaacttga gtcaagtaaa gaacagtttg
      841 ccagtacaaa cattgctgaa gagctagtaa aactcttcaa gaaacaaata gaacatgata
      901 agagagaaat gatttttgaa gttcttgctc cattggcaga aaatgatgct attaaactac
      961 agctggttga agcaggccta gtagagtgtc tactagagat tgttcagcaa aaagtggata
     1021 gtgacaaaga agatgatatt actgagctca aaactggttc agatctcatg gttttattac
     1081 ttcttggaga tgaatccatg cagaagttat ttgaaggagg aaaaggtagt gtatttcaaa
     1141 gggtactctc ttggatccca tcaaataacc accagctaca gcttgctgga gcattggcaa
     1201 ttgcaaattt tgccagaaat gatgcaaatt gtattcatat ggtagacaat gggattgtag
     1261 aaaaacttat ggatttactg gacagacatg tagaagatgg aaatgtaaca gtacagcatg
     1321 cagcactaag tgccctcaga aacctggcca ttccagttat aaataaagca aagatgttat
     1381 cagctggggt cacagaggca gttttgaaat ttcttaaatc tgaaatgcct cctgttcagt
     1441 tcaaacttct gggaacatta agaatgttaa tagatgcaca agcagaagct gctgaacaat
     1501 tgggaaagaa tgttaagtta gtggagcgtt tggtggaatg gtgtgaagcc aaagatcatg
     1561 ctggtgtgat gggggagtca aacagactgc tgtctgccct tatacgacac agtaaatcaa
     1621 aagatgtaat taaaaccatt gtgcagagtg gtggcatcaa gcatctagtt accatggcaa
     1681 ctagtgaaca tgtaataatg cagaatgaag ctcttgttgc tttggcatta atagcagctt
     1741 tagaattggg cactgctgag aaagatctag aaagtgctaa acttgtacag attttacata
     1801 gactgctagc agatgagaga agtgctcctg aaatcaaata taattccatg gtcctgatat
     1861 gtgctcttat gggatctgaa tgtctacaca aggaagtaca ggatttggct tttctagatg
     1921 tcgtatccaa acttcgcagt catgagaaca aaagtgttgc ccagcaggcc tctctcacag
     1981 agcagagact tactgtggaa agctgagaac tgcccgatac acggcatcat cccatctcta
     2041 atttcccctc tgtcctccat ccagcggctt cttccgcttc attctctacc ataccacttg
     2101 tgcatgcatg tgatgttcta ataccaattg aagaaccgct gtaggtacct ccctaataag
     2161 atttctaaac ctatagttag tgtgatcatg actttgtcaa aggcaagtct ccacccataa
     2221 ccgttctctt gtattcctgt tgcttgagct acattaagta gaatgtgcat gttgtagtcc
     2281 tatgatgatg taaacttggt actacataat gacttgctcc acacatgcag taaactacat
     2341 aatgatgtac tggtaaacta gaaacaaaga atgcagcagg atctgtctag cttattaaag
     2401 atgaaactga attggaaaaa tagctccatt ttttggtgct tgggaagcac agtgaccaaa
     2461 aaagttgtat ggctgcttat tcattagtct ttcctactga tgtcaaatcc atggtaccta
     2521 gagttaaata aaattccaat gctcttactc tttaacttct ggtttctcct ttttcctttt
     2581 tagactattg aaactgccac aaacttggca agactttctg aggtttctga ttccatattt
     2641 aattgactat tatcaatagc ctgatattga aaaacatttg tagttttcag tgtgaaacta
     2701 aggggttgaa gaatcactat tacaatccct attacagagc atttcgggtt ttgcttgtat
     2761 tttagattct gatacatacg ctgtttcact caggaactac ttctaccagt taatcagcat
     2821 tatccagcac ttgtctttaa aattcatttg gcgcttgttt gttttcaact gaggaaattg
     2881 agtatagttc attgaagaat ggaatttttt tttttttttt tttttttgct gtttttcatc
     2941 attcagctag aaagtgagaa gttttatctt ccatagtcct tttaaatctt atgttagaac
     3001 tagcaggacg taggtggaaa gatgaagaaa taagcttgtc tagactaaag aaaggaaagc
     3061 aggttataaa agccatagta gccagtccag ttcattctgc agatggcccc ataccaatcg
     3121 ctgttagagt ttgccacctc cactttccca ctgagaactg cagcaatttt aaaggatatg
     3181 agaaagccaa gactcaagag atgagaagac ccccttggcg aaatatagtg tactcttcac
     3241 tgccactgcc accaccaaat gggtttttag agctttgaaa cacaatcttt gtaaattaat
     3301 caaaacctcc ctgtgaccca cctgccttct ccccataccc aaatttgacc actactggcc
     3361 taaaaggcaa gatgggcttc gatatagaag gactgcaaga cagtagaaag ggctgccacg
     3421 gaggtgacag tagctccttc ctctccaaca tgtgatgcta cacatctctg tggataatct
     3481 aagttggact tttgactcta aaacagctat aatccaagaa agttaaattc taatggaggt
     3541 aaagaatctt gactgattgt tccctttgat gtccaaataa gggaactatt gtgagtacgt
     3601 atgctacact tactataaga atgatactgt ttgttgtctt tctcctgggc tggatagtgg
     3661 actatatttt attacaggct ttgaaagaca tctgtaatgt tgcatatctg ttcatattaa
     3721 taacttaata aatagtatat gaagcta
//
