LOCUS       NM_002885               3322 bp    mRNA    linear   PRI 02-APR-2024
DEFINITION  Homo sapiens RAP1 GTPase activating protein (RAP1GAP), transcript
            variant 3, mRNA.
ACCESSION   NM_002885
VERSION     NM_002885.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Han,M., Zhang,D., Ji,J., Zhang,J. and Qin,M.
  TITLE     Downregulating miR-184 relieves calcium oxalate crystal-mediated
            renal cell damage via activating the Rap1 signaling pathway
  JOURNAL   Aging (Albany NY) 15 (24), 14749-14763 (2023)
   PUBMED   38154105
  REMARK    GeneRIF: Downregulating miR-184 relieves calcium oxalate
            crystal-mediated renal cell damage via activating the Rap1
            signaling pathway.
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Faam,B., Ghadiri,A.A., Ghaffari,M.A., Totonchi,M., Amouzegar,A.,
            Azizi,F., Shahbazian,H., Hashemitabar,M., Fanaei,S.A. and
            Khorsandi,L.
  TITLE     CpG Island Methylation of the Rap1Gap Gene in Medullary Thyroid
            Cancer
  JOURNAL   Arch Iran Med 25 (3), 171-177 (2022)
   PUBMED   35429959
  REMARK    GeneRIF: CpG Island Methylation of the Rap1Gap Gene in Medullary
            Thyroid Cancer.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3322)
  AUTHORS   Shi,S., Li,J., Li,E., Guo,W., He,Y., Wang,J., Zhang,Y., Yue,L. and
            Wei,L.
  TITLE     Simulated Microgravity Increases the Permeability of HUVEC
            Monolayer through Up-Regulation of Rap1GAP and Decreased Rap2
            Activation
  JOURNAL   Int J Mol Sci 23 (2), 630 (2022)
   PUBMED   35054818
  REMARK    GeneRIF: Simulated Microgravity Increases the Permeability of HUVEC
            Monolayer through Up-Regulation of Rap1GAP and Decreased Rap2
            Activation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3322)
  AUTHORS   Yan,Z., Yangyanqiu,W., Shuwen,H., Jing,M., Haihong,L., Gong,C.,
            Yin,J., Qing,Z. and Weili,G.
  TITLE     Downregulation of Rap1GAP Expression Activates the TGF-beta/Smad3
            Pathway to Inhibit the Expression of Sodium/Iodine Transporter in
            Papillary Thyroid Carcinoma Cells
  JOURNAL   Biomed Res Int 2021, 6840642 (2021)
   PUBMED   34840979
  REMARK    GeneRIF: Downregulation of Rap1GAP Expression Activates the
            TGF-beta/Smad3 Pathway to Inhibit the Expression of Sodium/Iodine
            Transporter in Papillary Thyroid Carcinoma Cells.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3322)
  AUTHORS   Faam,B., Ghaffari,M.A., Khorsandi,L., Ghadiri,A.A., Totonchi,M.,
            Amouzegar,A., Fanaei,S.A., Azizi,F., Shahbazian,H.B. and Hashemi
            Tabar,M.
  TITLE     RAP1GAP Functions as a Tumor Suppressor Gene and Is Regulated by
            DNA Methylation in Differentiated Thyroid Cancer
  JOURNAL   Cytogenet Genome Res 161 (5), 227-235 (2021)
   PUBMED   34311462
  REMARK    GeneRIF: RAP1GAP Functions as a Tumor Suppressor Gene and Is
            Regulated by DNA Methylation in Differentiated Thyroid Cancer.
REFERENCE   6  (bases 1 to 3322)
  AUTHORS   Kurachi,H., Wada,Y., Tsukamoto,N., Maeda,M., Kubota,H., Hattori,M.,
            Iwai,K. and Minato,N.
  TITLE     Human SPA-1 gene product selectively expressed in lymphoid tissues
            is a specific GTPase-activating protein for Rap1 and Rap2.
            Segregate expression profiles from a rap1GAP gene product
  JOURNAL   J Biol Chem 272 (44), 28081-28088 (1997)
   PUBMED   9346962
REFERENCE   7  (bases 1 to 3322)
  AUTHORS   Peterson,S.N., Trabalzini,L., Brtva,T.R., Fischer,T.,
            Altschuler,D.L., Martelli,P., Lapetina,E.G., Der,C.J. and
            White,G.C. 2nd.
  TITLE     Identification of a novel RalGDS-related protein as a candidate
            effector for Ras and Rap1
  JOURNAL   J Biol Chem 271 (47), 29903-29908 (1996)
   PUBMED   8939933
REFERENCE   8  (bases 1 to 3322)
  AUTHORS   Weiss,J., Rubinfeld,B., Polakis,P.G., McCormick,F., Cavenee,W.K.
            and Arden,K.C.
  TITLE     The RAP1GA1 locus for human Rap1-GTPase activating protein 1 maps
            to chromosome 1p36.1-->p35
  JOURNAL   Cytogenet Cell Genet 66 (1), 18-21 (1994)
   PUBMED   8275700
REFERENCE   9  (bases 1 to 3322)
  AUTHORS   Rubinfeld,B., Crosier,W.J., Albert,I., Conroy,L., Clark,R.,
            McCormick,F. and Polakis,P.
  TITLE     Localization of the rap1GAP catalytic domain and sites of
            phosphorylation by mutational analysis
  JOURNAL   Mol Cell Biol 12 (10), 4634-4642 (1992)
   PUBMED   1406653
REFERENCE   10 (bases 1 to 3322)
  AUTHORS   Rubinfeld,B., Munemitsu,S., Clark,R., Conroy,L., Watt,K.,
            Crosier,W.J., McCormick,F. and Polakis,P.
  TITLE     Molecular cloning of a GTPase activating protein specific for the
            Krev-1 protein p21rap1
  JOURNAL   Cell 65 (6), 1033-1042 (1991)
   PUBMED   1904317
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY115754.1, M64788.1,
            AB007943.3, AL359815.26 and BC035030.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_002885.3.
            
            Summary: This gene encodes a type of GTPase-activating-protein
            (GAP) that down-regulates the activity of the ras-related RAP1
            protein. RAP1 acts as a molecular switch by cycling between an
            inactive GDP-bound form and an active GTP-bound form. The product
            of this gene, RAP1GAP, promotes the hydrolysis of bound GTP and
            hence returns RAP1 to the inactive state whereas other proteins,
            guanine nucleotide exchange factors (GEFs), act as RAP1 activators
            by facilitating the conversion of RAP1 from the GDP- to the
            GTP-bound form. In general, ras subfamily proteins, such as RAP1,
            play key roles in receptor-linked signaling pathways that control
            cell growth and differentiation. RAP1 plays a role in diverse
            processes such as cell proliferation, adhesion, differentiation,
            and embryogenesis. Alternative splicing results in multiple
            transcript variants encoding distinct proteins. [provided by
            RefSeq, Aug 2011].
            
            Transcript Variant: This variant (3) differs in the 5' UTR and
            lacks an alternate in-frame exon compared to variant 4. The
            resulting isoform (3) has the same N- and C-termini but is shorter
            compared to isoform 4. Variants 3 and 8 encode the same protein
            (isoform 3). Isoform 3 is also known as isoform c.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803612.100038.1,
                                           SRR1803615.160587.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2146411 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000374765.9/ ENSP00000363897.4
            RefSeq Select criteria :: based on manual assertion, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-51                HY115754.1         25-75
            52-570              M64788.1           1-519
            571-1337            AB007943.3         622-1388
            1338-1410           AL359815.26        33636-33708         c
            1411-1548           AL359815.26        32999-33136         c
            1549-1680           AL359815.26        30852-30983         c
            1681-1790           AL359815.26        27590-27699         c
            1791-1904           AL359815.26        26470-26583         c
            1905-2028           AL359815.26        24280-24403         c
            2029-2131           AL359815.26        23186-23288         c
            2132-2235           AL359815.26        22747-22850         c
            2236-2278           AL359815.26        22472-22514         c
            2279-3304           AL359815.26        21025-22050         c
            3305-3322           BC035030.1         3317-3334
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.12"
     gene            1..3322
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /note="RAP1 GTPase activating protein"
                     /db_xref="GeneID:5909"
                     /db_xref="HGNC:HGNC:9858"
                     /db_xref="MIM:600278"
     exon            1..104
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            105..140
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            141..234
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            235..270
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             253..2244
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /note="isoform 3 is encoded by transcript variant 3; rap1
                     GTPase-activating protein 1"
                     /codon_start=1
                     /product="rap1 GTPase-activating protein 1 isoform 3"
                     /protein_id="NP_002876.2"
                     /db_xref="CCDS:CCDS218.1"
                     /db_xref="GeneID:5909"
                     /db_xref="HGNC:HGNC:9858"
                     /db_xref="MIM:600278"
                     /translation="MIEKMQGSRMDEQRCSFPPPLKTEEDYIPYPSVHEVLGREGPFP
                     LILLPQFGGYWIEGTNHEITSIPETEPLQSPTTKVKLECNPTARIYRKHFLGKEHFNY
                     YSLDAALGHLVFSLKYDVIGDQEHLRLLLRTKCRTYHDVIPISCLTEFPNVVQMAKLV
                     CEDVNVDRFYPVLYPKASRLIVTFDEHVISNNFKFGVIYQKLGQTSEEELFSTNEESP
                     AFVEFLEFLGQKVKLQDFKGFRGGLDVTHGQTGTESVYCNFRNKEIMFHVSTKLPYTE
                     GDAQQLQRKRHIGNDIVAVVFQDENTPFVPDMIASNFLHAYVVVQAEGGGPDGPLYKV
                     SVTARDDVPFFGPPLPDPAVFRKGPEFQEFLLTKLINAEYACYKAEKFAKLEERTRAA
                     LLETLYEELHIHSQSMMGLGGDEDKMENGSGGGGFFESFKRVIRSRSQSMDAMGLSNK
                     KPNTVSTSHSGSFAPNNPDLAKAAGISLIVPGKSPTRKKSGPFGSRRSSAIGIENIQE
                     VQEKRESPPAGQKTPDSGHVSQEPKSENSSTQSSPEMPTTKNRAETAAQRAEALKDFS
                     RSSSSASSFASVVEETEGVDGEDTGLESVSSSGTPHKRDSFIYSTWLEDSVSTTSGGS
                     SPGPSRSPHPDAGKLGDPACPEIKIQLEASEQHMPQLGC"
     exon            271..318
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            319..357
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            358..543
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            544..647
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            648..726
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            727..780
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            781..864
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            865..965
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            966..1095
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1251
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1323
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1324..1410
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1548
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1549..1680
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1681..1790
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1791..1904
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1905..2028
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2029..2131
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2132..2235
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2236..2278
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2279..3322
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3297..3302
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /note="hexamer: AATAAA"
     polyA_site      3322
                     /gene="RAP1GAP"
                     /gene_synonym="RAP1GA1; RAP1GAP1; RAP1GAPII; RAPGAP"
                     /note="major polyA site"
ORIGIN      
        1 gaactgcggc ggcggcgagc gccggccgca tctgagcaga gctgcagcgg cggccgcggg
       61 caccagagtg ccgagcccag gacgcccccg gcccaggccc ttggggtgga caagtccttc
      121 acttctcgcc ggagtgtgtg gaggagcgat gggcagaacc agcacttccc tcaggcacta
      181 gacctgtcac gagtgaactt agttccctcc tatactcctt cactctaccc taagaacaca
      241 gatctatttg agatgattga gaagatgcag ggaagcagga tggatgaaca acgctgctcc
      301 ttcccgccgc ccctcaaaac agaggaggac tacattccat acccgagcgt gcacgaggtc
      361 ttggggcgag aaggaccctt ccccctcatc ctgctgcccc agtttggggg ctactggatt
      421 gagggcacca accacgaaat caccagcatc cccgagacag agccactgca gtcgcccaca
      481 accaaggtga agctcgagtg caaccccaca gcccgcatct accggaagca ctttctcggc
      541 aaggagcatt tcaattacta ctcactggac gctgccctcg gccaccttgt cttctcactc
      601 aagtacgatg tcatcgggga ccaagagcac ctgcggctgc tgctcaggac caagtgccgg
      661 acataccatg atgtcatccc catctcctgc ctcaccgagt tccctaatgt tgtccagatg
      721 gcaaagttgg tgtgtgaaga cgtcaatgtg gatcggttct atcctgtgct ctaccccaag
      781 gcttcccggc tcatcgtcac ctttgacgag catgtcatca gcaataactt caagtttggc
      841 gtcatttatc agaagcttgg gcagacctcc gaggaagaac tcttcagcac caatgaggaa
      901 agtcccgctt tcgtggagtt ccttgaattt cttggccaga aggtcaaact gcaggacttt
      961 aaggggttcc gaggaggcct ggacgtgacc cacgggcaga cggggaccga atctgtgtac
     1021 tgcaacttcc gcaacaagga gatcatgttt cacgtgtcca ccaagctgcc atacacggaa
     1081 ggggacgccc agcagttgca gcggaagcgg cacatcggga acgacatcgt ggctgtggtc
     1141 ttccaggatg agaacactcc tttcgtgccc gacatgatcg cgtccaactt cctgcatgcc
     1201 tacgtcgtgg tgcaggctga gggcgggggc cctgatggcc ccctctacaa ggtctctgtc
     1261 actgcaagag atgatgtgcc cttctttgga ccccccctcc cggaccccgc tgtgttcagg
     1321 aaggggcctg agttccagga atttttgctg acaaagctga tcaatgctga atatgcctgc
     1381 tacaaggcag agaagtttgc caaactggag gagcggacgc gggccgccct cctggagacg
     1441 ctctatgagg aactacacat ccacagccag tccatgatgg gcttgggcgg cgacgaggac
     1501 aagatggaga atggcagtgg gggcggcggc ttctttgagt ctttcaagcg ggtcatccgg
     1561 agccgcagcc agtccatgga tgccatgggg ctgagcaaca agaagcccaa caccgtgtcc
     1621 accagccaca gcgggagctt cgcgcccaac aaccccgacc tggccaaggc ggctggaata
     1681 tcactgattg tccctgggaa gagccccacg aggaagaagt cgggcccgtt cggctcccgc
     1741 cgcagcagcg ccattggcat cgagaacata caggaggtgc aggagaagag ggagagccct
     1801 ccggctggtc agaagacccc agacagcggg cacgtctcac aggagcccaa gtcggagaac
     1861 tcatccactc agagctcccc agagatgccc acgaccaaga acagagcgga gaccgcagcg
     1921 cagagagcag aggcgctcaa ggacttctcc cgctcctcgt ccagtgccag cagcttcgcc
     1981 agcgtggtgg aggagacgga gggtgtggac ggagaggaca caggcctgga gagcgtgtca
     2041 tcctcaggaa caccccacaa gcgggactcc ttcatctata gcacgtggct ggaggacagt
     2101 gtcagcacca ctagtggggg cagctcccca ggcccctctc gatcacccca cccagacgcc
     2161 ggcaagttgg gggaccctgc gtgtcccgag atcaagatcc agctggaagc atctgagcag
     2221 cacatgcccc agctgggctg ttagccgggc caccccctct gaaggtgaaa ctgagcagat
     2281 gaggccacag aagcacaagg ggaaggtgcc gtgtcaagcc caggcagacg agacctctgc
     2341 cctgaagacc aacaccagcc cgtgggctgc cccctgcctc cccaccctcc ccatggccca
     2401 cccatctggg ctgtctctgc agggcagagc cgtccagacc tgggatcagg gaagctgctg
     2461 gcatcgtccc cacccccagc ctgggggtct gggctggggc agggattgct cagtggaagc
     2521 aggactgggg gtctggcttg ccccctccct gggcctccat cacccctgag catccctctg
     2581 gactcagagg gaacaaggtg ggagagagag tttgagacag ctccgtgtgg agagcttagc
     2641 ccctggaggc agcacaagga ggatgtgata tgtgggggag tgagcactgg gttgggagcc
     2701 gggtcctggt ttccaatttg ggttctgctg tgtgactctg ggcaagtcac tctccctctc
     2761 tgggcatgtc tgctacaaat ggacaagatt atttcagagg tcactgaaga ctgtgattac
     2821 atgcacctgc cttagaaggt aggattttct tcccagggac ctcctatcac cctaccctgc
     2881 ttcttgaggt ccctggagcc ccaggtgggc tgaggggcag ggagccggct gtgcccagta
     2941 tgcctcctgg accctccagt tctgccacag gtctgccgat gccctgtcca ctgcctacac
     3001 atgacagaca agtaaccccc tcatggggga tggggaccta cctggctcct cagccagcac
     3061 ccagcttaac ccctgccatc ccatgctggg ccctccaggc caagagtctc agctggccga
     3121 gagtccaggc cttgcctccc ccgaccgcca tggagggggc agcccggcac agctgctggg
     3181 agcccttgtg tgtctggtca cactttttag gcgtcacgcc aaaggccagc ctcctggccc
     3241 caatacccat tttggaagcc cctgtggccg tgtggatgtc ggtaacagtt gtataaaata
     3301 aattctattt atcgctattg ta
//
