LOCUS       NM_002884               5018 bp    mRNA    linear   PRI 29-OCT-2024
DEFINITION  Homo sapiens RAP1A, member of RAS oncogene family (RAP1A),
            transcript variant 2, mRNA.
ACCESSION   NM_002884 XM_005271092 XM_011541883
VERSION     NM_002884.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Kosuru,R., Romito,O., Sharma,G.P., Ferraresso,F., Ghadrdoost
            Nakhchi,B., Yang,K., Mammoto,T., Mammoto,A., Kastrup,C.J.,
            Zhang,D.X., Goldspink,P.H., Trebak,M. and Chrzanowska,M.
  TITLE     Rap1A Modulates Store-Operated Calcium Entry in the Lung
            Endothelium: A Novel Mechanism Controlling NFAT-Mediated Vascular
            Inflammation and Permeability
  JOURNAL   Arterioscler Thromb Vasc Biol 44 (11), 2271-2287 (2024)
   PUBMED   39324266
  REMARK    GeneRIF: Rap1A Modulates Store-Operated Calcium Entry in the Lung
            Endothelium: A Novel Mechanism Controlling NFAT-Mediated Vascular
            Inflammation and Permeability.
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Liang,F., Rai,R., Sodeinde,T. and Chang,S.
  TITLE     TRF2-RAP1 represses RAD51-dependent homology-directed telomere
            repair by promoting BLM-mediated D-loop unwinding and inhibiting
            BLM-DNA2-dependent 5'-end resection
  JOURNAL   Nucleic Acids Res 52 (16), 9695-9709 (2024)
   PUBMED   39082275
  REMARK    GeneRIF: TRF2-RAP1 represses RAD51-dependent homology-directed
            telomere repair by promoting BLM-mediated D-loop unwinding and
            inhibiting BLM-DNA2-dependent 5'-end resection.
REFERENCE   3  (bases 1 to 5018)
  AUTHORS   Yu,L.R., Han,X.Z., Tang,Y.Z., Liu,D., Luo,X.Q., Qiu,X.W., Feng,J.,
            Yuan,W.X. and Ding,J.Y.
  TITLE     Activation of the MEK/ERK Pathway Mediates the Inhibitory Effects
            of Silvestrol on Nasopharyngeal Carcinoma Cells via RAP1A, HK2, and
            GADD45A
  JOURNAL   Front Biosci (Landmark Ed) 29 (4), 160 (2024)
   PUBMED   38682208
  REMARK    GeneRIF: Activation of the MEK/ERK Pathway Mediates the Inhibitory
            Effects of Silvestrol on Nasopharyngeal Carcinoma Cells via RAP1A,
            HK2, and GADD45A.
REFERENCE   4  (bases 1 to 5018)
  AUTHORS   Khan,A., Ni,W., Baltazar,T., Lopez-Giraldez,F., Pober,J.S. and
            Pierce,R.W.
  TITLE     ArhGEF12 activates Rap1A and not RhoA in human dermal microvascular
            endothelial cells to reduce tumor necrosis factor-induced leak
  JOURNAL   FASEB J 36 (4), e22254 (2022)
   PUBMED   35294066
  REMARK    GeneRIF: ArhGEF12 activates Rap1A and not RhoA in human dermal
            microvascular endothelial cells to reduce tumor necrosis
            factor-induced leak.
REFERENCE   5  (bases 1 to 5018)
  AUTHORS   Yamamoto,K., Takagi,Y., Ando,K. and Fukuhara,S.
  TITLE     Rap1 Small GTPase Regulates Vascular Endothelial-Cadherin-Mediated
            Endothelial Cell-Cell Junctions and Vascular Permeability
  JOURNAL   Biol Pharm Bull 44 (10), 1371-1379 (2021)
   PUBMED   34602545
  REMARK    GeneRIF: Rap1 Small GTPase Regulates Vascular
            Endothelial-Cadherin-Mediated Endothelial Cell-Cell Junctions and
            Vascular Permeability.
            Review article
REFERENCE   6  (bases 1 to 5018)
  AUTHORS   Hattori,M. and Minato,N.
  TITLE     Rap1 GTPase: functions, regulation, and malignancy
  JOURNAL   J Biochem 134 (4), 479-484 (2003)
   PUBMED   14607972
  REMARK    GeneRIF: Rap1 GTPase has functions in malignancy (review)
            Review article
REFERENCE   7  (bases 1 to 5018)
  AUTHORS   Quinn,M.T., Mullen,M.L., Jesaitis,A.J. and Linner,J.G.
  TITLE     Subcellular distribution of the Rap1A protein in human neutrophils:
            colocalization and cotranslocation with cytochrome b559
  JOURNAL   Blood 79 (6), 1563-1573 (1992)
   PUBMED   1312373
REFERENCE   8  (bases 1 to 5018)
  AUTHORS   Buss,J.E., Quilliam,L.A., Kato,K., Casey,P.J., Solski,P.A.,
            Wong,G., Clark,R., McCormick,F., Bokoch,G.M. and Der,C.J.
  TITLE     The COOH-terminal domain of the Rap1A (Krev-1) protein is
            isoprenylated and supports transformation by an H-Ras:Rap1A
            chimeric protein
  JOURNAL   Mol Cell Biol 11 (3), 1523-1530 (1991)
   PUBMED   1899909
REFERENCE   9  (bases 1 to 5018)
  AUTHORS   Rousseau-Merck,M.F., Pizon,V., Tavitian,A. and Berger,R.
  TITLE     Chromosome mapping of the human RAS-related RAP1A, RAP1B, and RAP2
            genes to chromosomes 1p12----p13, 12q14, and 13q34, respectively
  JOURNAL   Cytogenet Cell Genet 53 (1), 2-4 (1990)
   PUBMED   2108841
REFERENCE   10 (bases 1 to 5018)
  AUTHORS   Nagata,K., Itoh,H., Katada,T., Takenaka,K., Ui,M., Kaziro,Y. and
            Nozawa,Y.
  TITLE     Purification, identification, and characterization of two
            GTP-binding proteins with molecular weights of 25,000 and 21,000 in
            human platelet cytosol. One is the rap1/smg21/Krev-1 protein and
            the other is a novel GTP-binding protein
  JOURNAL   J Biol Chem 264 (29), 17000-17005 (1989)
   PUBMED   2507536
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BI460853.1, CD108761.1,
            BC014086.2, DA102623.1, CA446565.1, BX473371.1, BU189979.1,
            BU167449.1, BG743233.1, AK094737.1 and DB312010.1.
            
            On Aug 13, 2020 this sequence version replaced NM_002884.3.
            
            Summary: This gene encodes a member of the Ras family of small
            GTPases. The encoded protein undergoes a change in conformational
            state and activity, depending on whether it is bound to GTP or GDP.
            This protein is activated by several types of guanine nucleotide
            exchange factors (GEFs), and inactivated by two groups of
            GTPase-activating proteins (GAPs). The activation status of the
            encoded protein is therefore affected by the balance of
            intracellular levels of GEFs and GAPs. The encoded protein
            regulates signaling pathways that affect cell proliferation and
            adhesion, and may play a role in tumor malignancy. Pseudogenes of
            this gene have been defined on chromosomes 14 and 17. Alternative
            splicing results in multiple transcript variants. [provided by
            RefSeq, May 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660803.114685.1,
                                           SRR1163655.180191.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369709.4/ ENSP00000358723.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-36                BI460853.1         16-51
            37-678              CD108761.1         1-642
            679-1441            BC014086.2         551-1313
            1442-1989           DA102623.1         48-595
            1990-2515           CA446565.1         16-541              c
            2516-2897           BX473371.1         78-459
            2898-3412           BU189979.1         42-556
            3413-3666           BU167449.1         484-737
            3667-4249           BG743233.1         63-645
            4250-5015           AK094737.1         1717-2482
            5016-5018           DB312010.1         5-7                 c
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p13.2"
     gene            1..5018
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
                     /note="RAP1A, member of RAS oncogene family"
                     /db_xref="GeneID:5906"
                     /db_xref="HGNC:HGNC:9855"
                     /db_xref="MIM:179520"
     exon            1..146
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
                     /inference="alignment:Splign:2.1.0"
     exon            147..230
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
                     /inference="alignment:Splign:2.1.0"
     CDS             174..728
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
                     /EC_number="3.6.5.2"
                     /note="Ras-related protein Krev-1; Ras-related protein
                     Rap-1A; GTP-binding protein smg p21A"
                     /codon_start=1
                     /product="ras-related protein Rap-1A precursor"
                     /protein_id="NP_002875.1"
                     /db_xref="CCDS:CCDS840.1"
                     /db_xref="GeneID:5906"
                     /db_xref="HGNC:HGNC:9855"
                     /db_xref="MIM:179520"
                     /translation="MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQV
                     EVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILR
                     VKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYD
                     LVRQINRKTPVEKKKPKKKSCLLL"
     exon            231..299
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
                     /inference="alignment:Splign:2.1.0"
     exon            300..356
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
                     /inference="alignment:Splign:2.1.0"
     exon            357..497
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
                     /inference="alignment:Splign:2.1.0"
     exon            498..641
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
                     /inference="alignment:Splign:2.1.0"
     exon            642..757
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
                     /inference="alignment:Splign:2.1.0"
     exon            758..5018
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1349..1354
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
                     /note="hexamer: AATATA"
     polyA_site      1369
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
                     /note="major polyA site"
     regulatory      1420..1425
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
                     /note="hexamer: ATTAAA"
     polyA_site      1441
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
                     /note="major polyA site"
     regulatory      1474..1479
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
                     /note="hexamer: ATTAAA"
     polyA_site      1497
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
     regulatory      2495..2500
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
                     /note="hexamer: AATAAA"
     polyA_site      2515
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
     regulatory      4998..5003
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
                     /note="hexamer: AATAAA"
     polyA_site      5018
                     /gene="RAP1A"
                     /gene_synonym="C21KG; G-22K; KREV-1; KREV1; RAP1; SMGP21"
ORIGIN      
        1 gccgccgctc ccgaggcccc tgccgccgcc gctcccgctg ctgtcgccgc gcagagccgg
       61 agcaggagcc acggccgaga ggagggagga ggaggaggag gaggtggagg aggtggagga
      121 ggtggaggag gcgccggacc ggggggatcg tcagtattta aacagatcac atcatgcgtg
      181 agtacaagct agtggtcctt ggttcaggag gcgttgggaa gtctgctctg acagttcagt
      241 ttgttcaggg aatttttgtt gaaaaatatg acccaacgat agaagattcc tacagaaagc
      301 aagttgaagt cgattgccaa cagtgtatgc tcgaaatcct ggatactgca gggacagagc
      361 aatttacagc aatgagggat ttgtatatga agaacggcca aggttttgca ctagtatatt
      421 ctattacagc tcagtccacg tttaacgact tacaggacct gagggaacag attttacggg
      481 ttaaggacac ggaagatgtt ccaatgattt tggttggcaa taaatgtgac ctggaagatg
      541 agcgagtagt tggcaaagag cagggccaga atttagcaag acagtggtgt aactgtgcct
      601 ttttagaatc ttctgcaaag tcaaagatca atgttaatga gatattttat gacctggtca
      661 gacagataaa taggaaaaca ccagtggaaa agaagaagcc taaaaagaaa tcatgtctgc
      721 tgctctaggc ccatagtcag cagcagctct gagccagatt acaggaatga agaactgttg
      781 cctaattgga aagtgccagc attccagact tcaaaaataa aaaatctgaa gaggcttctc
      841 ctgttttata tattatgtga agaatttaga tcttatattg gtttgcacaa gttccctgga
      901 gaaaaaaatt gctctgtgta tatctcttgg aaaataagac aatagtattt ctcctttgca
      961 atagcagtta taacagatgt gaaaatatac ttgactctaa tatgattata caaaagagca
     1021 tggatgcatt tcaaatgtta gatattgcta ctataatcaa atgatttcat attgatcttt
     1081 ttatcatgat cctccctatc aagcactaaa aagttgaacc attatacttt atatctgtaa
     1141 tgatactgat tatgaaatgt cccctcaaac tcattgcagc agataacttt tttgagtcat
     1201 tgacttcatt ttatatttaa aaaattatgg aatatcatct gtcattatat tctaattaaa
     1261 attgtgcata atgctttgga aaaatgggtc ttttatagga aaaaaactgg gataactgat
     1321 ttctatggct ttcaaagcta aaatatataa tatactaaac caactctaat attgcttctt
     1381 gtgttttact gtcagattaa attacagctt ttatggatga ttaaatttta gtacattttc
     1441 atttggtttg tgtgtttttg ttattgttta tagattaaag cgtttatttt ataatgacca
     1501 cattgtttta aatgcgacag tagctccttt ctgcctagta tctgcagaac actggcttta
     1561 aactatacta agtaactggt gatttctcta ggaacagacc tcgcactttc tgttctaaat
     1621 atatttattc ctactataca gtaaaataca tcagacaaca tagaatagtt ttgtatattc
     1681 tctcttgatc ttaaagattg tatcttattg aatatcccac tgtatattat ttttatatgt
     1741 aaaaagataa gtttaatact gtatcagggt ttaactttta ctatttcaga tcttccacag
     1801 cttgtaattt catcaaggga atccttttgc attgttaatt tgttgggtgt gagtccacca
     1861 atgaagtgtt atgtgaaaaa tttcaaactt gctttataat agattgaaga atttgagaga
     1921 agtgagagaa agttaaaaca cataaaagga atgtgttact tttgcttaaa ctacttatga
     1981 aatagtatgg tagtagtaat atggaagaga ttgctaagtt aaaattgtag cttcctatat
     2041 agtggtgctt tttcttttcc ttcccagtta ttttcctctt tacaaaagat agggcttaag
     2101 taagacatgg acccagggtg atactactta tgtagtctct ataaagagag gtagaacaaa
     2161 aagcttgtta gccaattaag ttctggaaaa ggaaagaaac aagcttagaa aaccctgtgc
     2221 cttgtcctca aatattaagg aggagacaga ccagtcctag aaagtctaag tcattgctca
     2281 gggatctcta gatactgtct ctttgcaact aggtatgaag aagtgttcat gccttgctga
     2341 gattttccag gcatgtggtc agcctgtcac ttactaaact gaaattttaa aatcaggttt
     2401 caaatgttaa gctttgacca acctgagaaa atgttaaagt attgttttta aaaaaatgga
     2461 aatttttttt gtttgtgttt ttgttttagt ttcgaataaa cttagttacc ttacacttcc
     2521 cttctactta tccaattatg catatgtctg ggattgaggg gccaaaacta tagcgaactt
     2581 gaatgaagta ttagggccag ttgtcaggtc agggtggcat cttttatttt aaggtcctct
     2641 aaagtcattc tgctttaaaa cacccctcaa acataccttt aaacttaaga tcatagacag
     2701 acatctcatc aaccagtaaa actttctaaa tactaccttg tcacatctat tgtgtagtta
     2761 tttaacagat taaatagaag tcatttaata agactccaag tctcagtctg cttgactagt
     2821 agcataaact cagtctattc tattttagaa tgtggtggag aaagatgata gttattagat
     2881 tcctttgtca catcttatac agtccacttg ggttaaaggt ggaactgtca agaaaaacgc
     2941 ctttctggca cacagtgata tgcagagcag gaccagctga agtcagatgt gcaaggaaat
     3001 ctgtggttgc tgagcaggat gaaaagtatc ctgttagaaa gtgagatagg tgtgttttta
     3061 tattgtccta atgtgaactc cttagtcttg gttccaaagg gaagggtacc tgaaaataaa
     3121 tgtattttta tagctgaaaa atcaagagtt tctaggatat tgtgtgaatg tcaaatgata
     3181 aatatctaga aactgatttc atattagtat gtgtggctat ttaaagtata atgactttgg
     3241 tttggttata tgagtgacac agaatttaag gaggcttcat gccatagttc atagtttgct
     3301 ctaatggctt ggtggtatcc ctgaacagtc tacttttaga aattaacagt aaatacacaa
     3361 tccagctgta atcttaccct ccaccagggg gagcttcaac atcggttaat actttttttt
     3421 tttttgagat ggagtctcgc cctgtcgccc aggctagagt gcaatggctc agtctccgct
     3481 cactgcaacc tctgcctccc gggttcaaac agttgtgcct cagtcttcca agtagctggg
     3541 attacaggca cccgccacca cgcccagcta atttttgtat ttttagtaga gacggggttt
     3601 catcatgttg gcgaggctgg tcttgaactc ctgacttcgt gatccgcccc cccctcagcc
     3661 tcccaaagtg ctgggattac aggcgtgagc caccgcgcct ggcccggtta atactttcat
     3721 aagcattgaa gtaactattt aagtcctagt acataaacac tgtgtggttc acaaaaaggc
     3781 atagagacat tctctcaaga gtttacaatc taattgggca gggaagatac tacagttcat
     3841 caaagatatg aataacaccc tagttttctg aactgcagct gcatgggtaa ttatcttaag
     3901 aacttttggc aaaatatgga tgttggtgtg ttactctctt ctccctacaa ctctgaacta
     3961 atctgtgttt ggcaccttga tttgccatca taaatggcca ccatttactc ctcactgaaa
     4021 tattcttctt ctttgaggtt ataatgtact tggaaaagtt aatacttata gaaaataatg
     4081 atctttatta ccaactttat taaaacaggc ttggaacatt tttctccata gaagaatagt
     4141 aataagacat tctacatttc ctggacatgg ccagtttaaa atattttgcc ccattaccct
     4201 tgtagataca catgtatttg tcagtttatg tgcctcagtt ttgtcttagg cactgaggga
     4261 cagaaagaga aataaggaat accttggtat tttcctttgc aagcaatcac agcagaatgc
     4321 tagaaaacat aaaatatact cttataaatt gtatgtgtgt tgaaaccaaa gtgtgaagaa
     4381 attgtcaggt atttggagga ggaaaataat ttgtaaaata acctttttaa gaatttggat
     4441 tttgtactta tttaaggtgc taaatatgtg ttaaaatatc ccctcttgaa tcagctccag
     4501 gcttaaaagg gcagagtggt ttggattctt gtccagggtc tggaaagagc tcatcttgcc
     4561 gagagggaaa taaatgtgag ctttgaggcg gtctaactac ccagctttta gtatttatgt
     4621 tccccaaaat gtaaaaactg taagttattc ttgaaaatgt agtccagaca tttctttgat
     4681 cctggatttt actgcagttt catcatgggt ttgttgaatg tgcagtccat ttattgccct
     4741 gtcttgctta aatattcttg ttgagaacag ctggaggctt tgactgggtt cctagttctg
     4801 ttttctggga aggcaacgtg gattctgttt tgtagacatt aatgagcttt aaatgggaat
     4861 tggggatgat gtatgttcgt tgggcatgct gagaagcggc ctcctgtggt tctgggccca
     4921 aggtgtgaca tacatttccc actaattgct tctctcaaga agcaagagat tggctgttcc
     4981 tcttgaggaa aaaccttaat aaaccttccc attttaaa
//
