LOCUS       NM_031452               1565 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens RNA guanine-7 methyltransferase activating subunit
            (RAMAC), mRNA.
ACCESSION   NM_031452
VERSION     NM_031452.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 1565)
  AUTHORS   Bueren-Calabuig,J.A., G Bage,M., Cowling,V.H. and Pisliakov,A.V.
  TITLE     Mechanism of allosteric activation of human mRNA cap
            methyltransferase (RNMT) by RAM: insights from accelerated
            molecular dynamics simulations
  JOURNAL   Nucleic Acids Res 47 (16), 8675-8692 (2019)
   PUBMED   31329932
  REMARK    GeneRIF: Mechanism of allosteric activation of human RNMT by RAM
            has been reported.
REFERENCE   4  (bases 1 to 1565)
  AUTHORS   Fasci,D., van Ingen,H., Scheltema,R.A. and Heck,A.J.R.
  TITLE     Histone Interaction Landscapes Visualized by Crosslinking Mass
            Spectrometry in Intact Cell Nuclei
  JOURNAL   Mol Cell Proteomics 17 (10), 2018-2033 (2018)
   PUBMED   30021884
REFERENCE   5  (bases 1 to 1565)
  AUTHORS   Dunn,S., Lombardi,O. and Cowling,V.H.
  TITLE     c-Myc co-ordinates mRNA cap methylation and ribosomal RNA
            production
  JOURNAL   Biochem J 474 (3), 377-384 (2017)
   PUBMED   27934633
  REMARK    GeneRIF: RNMT-RAM complex coordinates mRNA processing with ribosome
            production.
REFERENCE   6  (bases 1 to 1565)
  AUTHORS   Varshney,D., Petit,A.P., Bueren-Calabuig,J.A., Jansen,C.,
            Fletcher,D.A., Peggie,M., Weidlich,S., Scullion,P., Pisliakov,A.V.
            and Cowling,V.H.
  TITLE     Molecular basis of RNA guanine-7 methyltransferase (RNMT)
            activation by RAM
  JOURNAL   Nucleic Acids Res 44 (21), 10423-10436 (2016)
   PUBMED   27422871
REFERENCE   7  (bases 1 to 1565)
  AUTHORS   Gonatopoulos-Pournatzis,T. and Cowling,V.H.
  TITLE     RAM function is dependent on Kapbeta2-mediated nuclear entry
  JOURNAL   Biochem J 457 (3), 473-484 (2014)
   PUBMED   24200467
  REMARK    GeneRIF: Data suggest that RAM/FAM103A1 has three domains:
            N-terminal activation domain (RAD); central RNA-binding domain (NR
            domain); and C-terminal nuclear localization domain (QYP) which
            contains two nuclear localization signal sequences.
REFERENCE   8  (bases 1 to 1565)
  AUTHORS   Baltz,A.G., Munschauer,M., Schwanhausser,B., Vasile,A.,
            Murakawa,Y., Schueler,M., Youngs,N., Penfold-Brown,D., Drew,K.,
            Milek,M., Wyler,E., Bonneau,R., Selbach,M., Dieterich,C. and
            Landthaler,M.
  TITLE     The mRNA-bound proteome and its global occupancy profile on
            protein-coding transcripts
  JOURNAL   Mol Cell 46 (5), 674-690 (2012)
   PUBMED   22681889
REFERENCE   9  (bases 1 to 1565)
  AUTHORS   Gonatopoulos-Pournatzis,T., Dunn,S., Bounds,R. and Cowling,V.H.
  TITLE     RAM/Fam103a1 is required for mRNA cap methylation
  JOURNAL   Mol Cell 44 (4), 585-596 (2011)
   PUBMED   22099306
  REMARK    GeneRIF: RAM is an essential component of the core gene expression
            machinery
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC022558.9, BC112329.1 and BQ002618.1.
            
            On Nov 23, 2018 this sequence version replaced NM_031452.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.138270.1,
                                           SRR1660809.60487.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000304191.4/ ENSP00000307181.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AC022558.9         42686-42709         c
            25-1182             BC112329.1         1-1158
            1183-1484           AC022558.9         37943-38244         c
            1485-1565           BQ002618.1         17-97               c
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q25.2"
     gene            1..1565
                     /gene="RAMAC"
                     /gene_synonym="C15orf18; FAM103A1; HsT19360; RAM; RAMMET"
                     /note="RNA guanine-7 methyltransferase activating subunit"
                     /db_xref="GeneID:83640"
                     /db_xref="HGNC:HGNC:31022"
                     /db_xref="MIM:614547"
     exon            1..160
                     /gene="RAMAC"
                     /gene_synonym="C15orf18; FAM103A1; HsT19360; RAM; RAMMET"
                     /inference="alignment:Splign:2.1.0"
     exon            161..209
                     /gene="RAMAC"
                     /gene_synonym="C15orf18; FAM103A1; HsT19360; RAM; RAMMET"
                     /inference="alignment:Splign:2.1.0"
     exon            210..388
                     /gene="RAMAC"
                     /gene_synonym="C15orf18; FAM103A1; HsT19360; RAM; RAMMET"
                     /inference="alignment:Splign:2.1.0"
     CDS             219..575
                     /gene="RAMAC"
                     /gene_synonym="C15orf18; FAM103A1; HsT19360; RAM; RAMMET"
                     /note="protein FAM103A1; RNMT-activating mini protein;
                     family with sequence similarity 103 member A1; RNMT
                     activating mRNA cap methyltransferase subunit"
                     /codon_start=1
                     /product="RNA guanine-N7 methyltransferase activating
                     subunit"
                     /protein_id="NP_113640.1"
                     /db_xref="CCDS:CCDS10321.1"
                     /db_xref="GeneID:83640"
                     /db_xref="HGNC:HGNC:31022"
                     /db_xref="MIM:614547"
                     /translation="MTDTAEAVPKFEEMFASRFTENDKEYQEYLKRPPESPPIVEEWN
                     SRAGGNQRNRGNRLQDNRQFRGRDNRWGWPSDNRSNQWHGRSWGNNYPQHRQEPYYPQ
                     QYGHYGYNQRPPYGYY"
     exon            389..1565
                     /gene="RAMAC"
                     /gene_synonym="C15orf18; FAM103A1; HsT19360; RAM; RAMMET"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1157..1162
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAMAC"
                     /gene_synonym="C15orf18; FAM103A1; HsT19360; RAM; RAMMET"
                     /note="hexamer: AATAAA"
     polyA_site      1182
                     /gene="RAMAC"
                     /gene_synonym="C15orf18; FAM103A1; HsT19360; RAM; RAMMET"
                     /note="major polyA site"
     regulatory      1527..1532
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAMAC"
                     /gene_synonym="C15orf18; FAM103A1; HsT19360; RAM; RAMMET"
                     /note="hexamer: AATAAA"
     polyA_site      1565
                     /gene="RAMAC"
                     /gene_synonym="C15orf18; FAM103A1; HsT19360; RAM; RAMMET"
ORIGIN      
        1 aggtggttgt cggattttag aggaaggcgc tcggttacat tggagaactg gagtggtctg
       61 gagttccacg gtgtagtgga ccagaggcca cctctcctgg gcttctcagt gtctcgccgg
      121 cggggttcgg cctgagctgg attgacatag cccttggcgg atttaaacaa cctaaacatt
      181 aagcagtaca gctgcctcaa acctttggga ttttcagaat gactgacact gccgaagctg
      241 ttccaaagtt tgaagagatg tttgctagta gattcacaga aaatgacaag gagtatcagg
      301 aatacctgaa acgccctcct gagtctcctc caattgttga ggaatggaat agcagagctg
      361 gtgggaacca aagaaacaga ggcaatcggt tgcaagacaa cagacagttc agaggcaggg
      421 acaacagatg ggggtggcca agtgacaatc gatccaatca gtggcatgga cgatcctggg
      481 gtaacaacta cccgcaacac agacaagaac cttactatcc ccagcaatat ggacattatg
      541 gttacaacca gcggcctcct tacggttact actgatagaa atgttggcag cttttagtaa
      601 aagcatttac tctgttacca tgagaaaagt ttgggtgtct tctgttggtc atagttttac
      661 atctgatttt acagaatgga ttattgattt tttggaagtt gagactttaa aaaaaataga
      721 tcttacttgc gaaatgcgat ggttgctggg aatacctgaa actgtggatt atattgcttg
      781 acttctacct cagaatcttc tttgtttcat gacttaatag tgctttaagt ttggtatatt
      841 atttgacctc taggaattct ttgttttaca cagaaataaa aattttaaaa tagaaaatgc
      901 ttttactttg taaggtaaga gagtatccat atgcttagat gtgctcgttt ctaaaattct
      961 agaggttgat ataatcagct catgaatgca cagctatgct ttttgtgata gattgtacat
     1021 aacatcagca gttgaaaggt aaaacaattg cttttttttt tttttgcatt tgttaagtga
     1081 ctatggtact ttgtgattcc ttaatctata gatgagtcag ctccacactt gagtctcttt
     1141 ttagagggaa atcagtaata aagctgtaaa ataaggaagg aatcattgtc agtttgtgtc
     1201 ttgatctggt ggtggttggg ataagggtac acatcatttt atacaaacac taaagctttt
     1261 tgctaacaac atagcaggtc aaaattcaca cataagaaag tttaactctg tactgttcac
     1321 actttttaat cctgtaaata atgctgcatt ttgatggttc ttactttttt ggtatttaat
     1381 gtgggctgat tttacaatgt tatattcact tccagatgca tacctctgct gctttctctc
     1441 ctataaatag tgatgctata aacttccttt cacagagatc attctatttc aaatcacttt
     1501 aatgattatt acaatgattt cattcaaata aaaattttaa aatatttaag catcttcact
     1561 cccaa
//
