LOCUS       NM_152663               7492 bp    mRNA    linear   PRI 03-OCT-2024
DEFINITION  Homo sapiens Ral GEF with PH domain and SH3 binding motif 2
            (RALGPS2), transcript variant 10, mRNA.
ACCESSION   NM_152663
VERSION     NM_152663.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 7492)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 7492)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 7492)
  AUTHORS   D'Aloia,A., Berruti,G., Costa,B., Schiller,C., Ambrosini,R.,
            Pastori,V., Martegani,E. and Ceriani,M.
  TITLE     RalGPS2 is involved in tunneling nanotubes formation in 5637
            bladder cancer cells
  JOURNAL   Exp Cell Res 362 (2), 349-361 (2018)
   PUBMED   29208460
  REMARK    GeneRIF: In fact the overexpression of RalGPS2 or of its PH-domain
            increased markedly the number and the length of nanotubes, while
            the knock-down of RalGPS2 caused a strong reduction of these
            structures. Moreover, using a series of RalA mutants impaired in
            the interaction with different downstream components (Sec5, Exo84,
            RalBP1) we demonstrated that the interaction of RalA with Sec5 is
            required for TNTs formation
REFERENCE   5  (bases 1 to 7492)
  AUTHORS   O Santos,A., Parrini,M.C. and Camonis,J.
  TITLE     RalGPS2 Is Essential for Survival and Cell Cycle Progression of
            Lung Cancer Cells Independently of Its Established Substrates Ral
            GTPases
  JOURNAL   PLoS One 11 (5), e0154840 (2016)
   PUBMED   27149377
  REMARK    GeneRIF: RALGPS2 is implicated in the control of cell cycle
            progression and survival in the in vitro growth of NSCLC cell
            lines. This function is largely independent of Ral GTPases and
            associated with modulation of Skp2, p27 and p21 cell cycle
            regulators
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7492)
  AUTHORS   Benzinger,A., Muster,N., Koch,H.B., Yates,J.R. 3rd and Hermeking,H.
  TITLE     Targeted proteomic analysis of 14-3-3 sigma, a p53 effector
            commonly silenced in cancer
  JOURNAL   Mol Cell Proteomics 4 (6), 785-795 (2005)
   PUBMED   15778465
REFERENCE   7  (bases 1 to 7492)
  AUTHORS   Jin,J., Smith,F.D., Stark,C., Wells,C.D., Fawcett,J.P.,
            Kulkarni,S., Metalnikov,P., O'Donnell,P., Taylor,P., Taylor,L.,
            Zougman,A., Woodgett,J.R., Langeberg,L.K., Scott,J.D. and Pawson,T.
  TITLE     Proteomic, functional, and domain-based analysis of in vivo 14-3-3
            binding proteins involved in cytoskeletal regulation and cellular
            organization
  JOURNAL   Curr Biol 14 (16), 1436-1450 (2004)
   PUBMED   15324660
REFERENCE   8  (bases 1 to 7492)
  AUTHORS   Martegani,E., Ceriani,M., Tisi,R. and Berruti,G.
  TITLE     Cloning and characterization of a new Ral-GEF expressed in mouse
            testis
  JOURNAL   Ann N Y Acad Sci 973, 135-137 (2002)
   PUBMED   12485849
REFERENCE   9  (bases 1 to 7492)
  AUTHORS   Quilliam,L.A., Rebhun,J.F. and Castro,A.F.
  TITLE     A growing family of guanine nucleotide exchange factors is
            responsible for activation of Ras-family GTPases
  JOURNAL   Prog Nucleic Acid Res Mol Biol 71, 391-444 (2002)
   PUBMED   12102558
  REMARK    Review article
REFERENCE   10 (bases 1 to 7492)
  AUTHORS   Rebhun,J.F., Chen,H. and Quilliam,L.A.
  TITLE     Identification and characterization of a new family of guanine
            nucleotide exchange factors for the ras-related GTPase Ral
  JOURNAL   J Biol Chem 275 (18), 13406-13410 (2000)
   PUBMED   10747847
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL449106.15, BC047391.1, AL355520.8 and CK902157.1.
            
            On Jun 2, 2019 this sequence version replaced NM_152663.4.
            
            Transcript Variant: This variant (10 represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.4010480.1,
                                           SRR11853561.2215.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000367635.8/ ENSP00000356607.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                AL449106.15        68904-68929
            27-2260             BC047391.1         109-2342
            2261-7045           AL355520.8         106450-111234
            7046-7492           CK902157.1         16-462              c
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q25.2"
     gene            1..7492
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /note="Ral GEF with PH domain and SH3 binding motif 2"
                     /db_xref="GeneID:55103"
                     /db_xref="HGNC:HGNC:30279"
                     /db_xref="MIM:617819"
     exon            1..176
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            177..316
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     CDS             260..2011
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /note="isoform 1 is encoded by transcript variant 10;
                     Ral-A exchange factor RalGPS2; ras-specific guanine
                     nucleotide-releasing factor RalGPS2; ral GEF with PH
                     domain and SH3-binding motif 2; ralA exchange factor
                     RalGPS2"
                     /codon_start=1
                     /product="ras-specific guanine nucleotide-releasing factor
                     RalGPS2 isoform 1"
                     /protein_id="NP_689876.2"
                     /db_xref="CCDS:CCDS1325.1"
                     /db_xref="GeneID:55103"
                     /db_xref="HGNC:HGNC:30279"
                     /db_xref="MIM:617819"
                     /translation="MDLMNGQASSVNIAATASEKSSSSESLSDKGSELKKSFDAVVFD
                     VLKVTPEEYAGQITLMDVPVFKAIQPDELSSCGWNKKEKYSSAPNAVAFTRRFNHVSF
                     WVVREILHAQTLKIRAEVLSHYIKTAKKLYELNNLHALMAVVSGLQSAPIFRLTKTWA
                     LLSRKDKTTFEKLEYVMSKEDNYKRLRDYISSLKMTPCIPYLGIYLSDLTYIDSAYPS
                     TGSILENEQRSNLMNNILRIISDLQQSCEYDIPMLPHVQKYLNSVQYIEELQKFVEDD
                     NYKLSLKIEPGTSTPRSAASREDLVGPEVGASPQSGRKSVAAEGALLPQTPPSPRNLI
                     PHGHRKCHSLGYNFIHKMNTAEFKSATFPNAGPRHLLDDSVMEPHAPSRGQAESSTLS
                     SGISIGSSDGSELSEETSWPAFERNRLYHSLGPVTRVARNGYRSHMKASSSAESEDLA
                     VHLYPGAVTIQGVLRRKTLLKEGKKPTVASWTKYWAALCGTQLFYYAAKSLKATERKH
                     FKSTSNKNVSVIGWMVMMADDPEHPDLFLLTDSEKGNSYKFQAGNRMNAMLWFKHLSA
                     ACQSNKQQVPTNLMTFE"
     exon            317..421
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            422..472
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            473..556
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            557..646
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            647..739
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            740..866
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            867..1004
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1095
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1096..1163
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1164..1299
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1300..1451
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1452..1506
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1507..1584
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1585..1690
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1691..1783
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1784..1889
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1890..1981
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     exon            1982..7492
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5734..5739
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /note="hexamer: AATATA"
     polyA_site      5755
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /note="major polyA site"
     regulatory      7474..7479
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
                     /note="hexamer: AATAAA"
     polyA_site      7492
                     /gene="RALGPS2"
                     /gene_synonym="dJ595C2.1"
ORIGIN      
        1 actctcctcc cccgagcggc agcggcggcg gcggcggcgg ctgctgcggg cgctgaatga
       61 gagacggtga ctgttcgggt cgacgagtgc tactctaggc ggcggcggcc gtggcggtga
      121 agcgtgaggc cggcatcgtc tttccgtcct ctgaggcgac ggccgcggct gcacaggaat
      181 aatgtatttg tggccttgga catgaggcag tcagtcctct gttgctgtta acataaggtc
      241 agggactgat gaggaaagca tggacctaat gaacgggcag gcaagcagtg tcaatattgc
      301 agctactgct tctgagaaaa gtagcagctc tgaatcctta agtgacaaag gctctgaatt
      361 gaagaaaagc tttgatgctg tggtattcga tgttcttaag gttacaccag aagaatatgc
      421 gggtcagata acattaatgg atgttccagt atttaaagct attcaaccag atgagctttc
      481 aagttgtgga tggaataaaa aagaaaaata tagttctgca ccaaatgcag ttgccttcac
      541 aagaagattc aatcatgtaa gcttttgggt tgttagagag attcttcatg ctcaaacatt
      601 aaaaattaga gcagaagttt tgagccacta tattaaaact gctaagaaac tgtatgagct
      661 gaataacctt catgcactta tggcagtggt ttctggccta cagagtgccc caattttcag
      721 gttgactaaa acatgggcgt tattaagtcg aaaagacaaa actacctttg aaaaattaga
      781 atatgtaatg agtaaagaag ataactacaa aagactcaga gactatataa gtagcttaaa
      841 gatgacacct tgcattccct atttaggtat ctatttgtca gatttaacat acatcgattc
      901 agcataccca tcaactggca gcattctaga aaatgagcaa agatcaaatt taatgaataa
      961 tatccttcga ataatttctg atttacagca gtcttgtgaa tatgatattc ccatgttgcc
     1021 tcatgtccaa aaatatctca actctgttca gtatatagaa gaactacaaa aatttgtgga
     1081 agacgataat tacaagcttt cattaaagat agaaccaggg acaagcaccc cacgttctgc
     1141 tgcttccaga gaagatttag taggtcctga agtaggagcg tctccacaga gtggacgaaa
     1201 aagtgtggca gctgaaggag ccttgctccc acagacaccg ccatcccctc ggaatctgat
     1261 tccacatgga cataggaagt gccatagttt gggttataat ttcattcata aaatgaacac
     1321 agcagaattt aagagtgcaa cgtttccaaa tgcaggacca agacatctgt tagatgatag
     1381 cgtcatggag ccccatgcgc catctcgagg ccaagctgaa agttctactc tttctagtgg
     1441 aatatcaata ggtagcagcg atggttctga actaagtgaa gagacctcat ggcctgcttt
     1501 tgaaaggaac agattatacc attctctcgg cccggtgaca agagtggcac gaaatggcta
     1561 tcgaagtcac atgaaggcca gcagttctgc agaatcagaa gatttggcag tacatttata
     1621 tccaggagct gttactattc aaggtgttct caggagaaaa actttgttaa aagaaggcaa
     1681 aaagcctaca gtagcatctt ggacaaaata ttgggcagct ttgtgtggga cacagctttt
     1741 ttactatgct gccaaatctc taaaggctac cgaaagaaaa catttcaaat caacatccaa
     1801 taagaacgta tctgtgatag gatggatggt gatgatggct gatgaccctg aacatcctga
     1861 tctcttcctg ctgactgact ctgagaaagg aaattcgtac aagtttcaag ctggcaatag
     1921 aatgaatgca atgttatggt ttaagcattt gagtgcagcc tgccaaagta acaaacaaca
     1981 ggttcctaca aacttgatga cttttgagta gaagcctgag aaaaaaagag aggtgaactg
     2041 ttgcttctac gtgagcatga ggacctgata aaagagcgcc agctataaac catcctgtgc
     2101 caggaagagc ccagaggctc tgtctcagtc gttggagttc tcaaccaaaa aagcactaat
     2161 ttcagggaat gatttgagat attcccagag aacaaattgg agttgcaaaa caaactgcca
     2221 tgaaccatgc ttcttatctc agaactgacc tgtggaaacc actgccttaa aagaatgaaa
     2281 ggaaaaccaa catgaaacac caaatagtgt gtgtgaatct tctggcggtg ctgtgcttgg
     2341 aatagatcgt agctaatttg catttctttt aactttgtaa taattttaga ggggggaatt
     2401 gcctttttta gatatgcttt aaaaaggaaa aacatactct tgtggattcc atcaggagct
     2461 ggttttgaac cgaggtgaag agacttagca gggattgtac cagcagccca cctgcaaggt
     2521 ttgttctccc tctagtagtc tgcaacctca ctggctttct cacagcaaga gtgtaggaat
     2581 gagaaatgtt agtcatgtga gttcttgggt ttgtttggga ttgggtgggg gtgtttgttt
     2641 tgttttggat gaggggagaa tttttttaaa cactaaactt ctgaaatgta gtactgtaag
     2701 gggaacatta tttcctgcag gaggtacaaa ggctgtgtgt tcatttgcca gacgcttttt
     2761 ttttaaatag gttccagaaa acatggttgt ttctaactag ggttaaagtt tagctttcta
     2821 tatgaattca ttgttggacc acagatctgc ttaagtaaag agctataatc ccaacctaaa
     2881 cttttctgag attttacaat aattagatct ttttccaagt taattgggtt ttcccttctc
     2941 ccagtcatag gtggttttta tcatcaagac agactgatat tttgtcagga tattttcttt
     3001 tacagtgttt gatgtgcata atgccagagt tattttttta ttattcattt tctctctttt
     3061 tgttcaatat gagattcagg atcatatttg tttaaaaggt aacacataga gatgtatgta
     3121 tatattttgt tataagacat acaaaataat tttaagaggg ataaaggtga aaatatcaga
     3181 ttctggaaat tttaagtatc taaactttat acttgtatga tttaccataa acataccaaa
     3241 acatttttct gaaaatttac tgtcggtctc tgacatgaaa ccgtattttg tcagtagttg
     3301 accaagcagt tttatgagaa ctcttctatg caatgatgca tttaatttct aaagtaaatg
     3361 ttgaattatt aaaaaaaaag tttgaaggta agatgcctta gaattttaat gaaaaatgac
     3421 actcaaacaa aggctatatg tcgaatgtta taaactcttg agacataatg aattttagca
     3481 gtggcctaga gtaaggtaat gctttgtgac tgatatctac taatagactt ctgttacttt
     3541 gggcctcaca taaaatttct cacattcgta ttcttagaga attttatact tttttattat
     3601 aagtattttg cccttgagtc catgaaactt caatagaaaa tattgcttat acaataatta
     3661 gcttaaaatc catccaaagg aaaagagaag agcttttttt gagtgttaag ttttttaatt
     3721 tagtattctg tttggaaaag atgttaagag ttcaaatatg cttcttatgt aacagaaata
     3781 ccattatttc tggggtttag tgacacaggc tggagaggac aaaagtttcc tgtcctgaat
     3841 gatttttcaa ttaatgctct aagcttaacc catgttgggt aatagaaaga catagccagc
     3901 agtattacta cgtagacctt gcacatctta cttttgtttt gcatttttaa aaatttctta
     3961 atttgtttat ttctcttcag aaatagcgtt tttaacagtg tatgtgcttt tttaaaaagt
     4021 attgtgaatg agaaccctgt ggattttttt tatttcattt gtttgtcata ataagctaac
     4081 gtagaaatgt tgtaggttaa ccatatctgt ggaaatatgc agttggaagt cagagcctga
     4141 catcacatag ttttgccaaa gggagagagc taagctggaa gatgtttttg atactttcgg
     4201 ctctaaacat taatggatgt ttgcgtagag taagatagcg ttcaaatttg gttaatagtt
     4261 tatagataga cttgtggcct ctgactgact aaatttctta acagcacaga agtcgttgtt
     4321 tttctgtctt taaaatttat tcattcagaa aattagtcat tgctttttaa gattatttta
     4381 gcatttgaag caaattgaaa acagaaaagt gacttaaaat agtgtaggtg aattttttat
     4441 ctataattta ccaaataata cctgataata attcaaagaa aatatgttac caaaatatat
     4501 taaatatata ggtagttcat tcgtgggaag atgaatcttc ttattaagcc tgccctcaat
     4561 tgtaaataag tgactcaaaa actcaaaatg ttcatcagca ttcaggtcat ttgtttcacc
     4621 cttagccaag aagaactcat tagaaattca gttataattt caatttaagc ctgtttatga
     4681 taagcataat gattggcatg ttatatagcc tgttgaaaat aactacagaa tgaccttcca
     4741 gagcactgtt tttggcctcg tgcccgctct gttcaggctc tgcttgtttt tcagaatatg
     4801 atttttacct ccagacagta agaattgaac aagaactgat caaacacacc ttcctatttt
     4861 gttttcttca ctatgtaaat attatttggg caaacattac aaaaagggat ccaaacaatt
     4921 tgagtttcag tatttttttc tccctaggca tgtgcttcaa tttcaaatat ttttttctgt
     4981 gtggaaaatg ccatgtaact attgcactac cttctgtgtg gactgtgttg gtattggttc
     5041 tttctgtata tcaaaagtca gggtgtttat aactgcaaag tttgtataag tggctttatt
     5101 cctcttctag aagatttatg aggagcctag gaagcactat tggtacagga ggaaaaaaga
     5161 atggaaaaca tgtttacaga ctctagcttt ccttattagc ttaaactggg gccctcaaag
     5221 agcagcctgt tgatcttttg gccaaatgac ttgagcataa gtcttttatt tctttttaag
     5281 gttaaatcgt aaactgtata cctttactac tgaaaacaaa actatagggc atcatactaa
     5341 ttgaaaatca atagtaatgg gttccatcca ttagcttttt aaatgaatag atctggtatt
     5401 gatttccttc ctgttttttt gaggcacatt cctttacaac cagtgtttaa accaccacgt
     5461 aatcatcttc tgcaaacaag gggtgccagt gttgcctaac agaagataat ctttaataaa
     5521 gcaaatcctc tgcttcaaag gtttttgaaa taattggatc cctttttgaa aaggaagatg
     5581 agtttaactg tgtccaggtg gagtaatagt actgctgttg catgaataga tgatacaaag
     5641 caagtgatga ggttggtatg acttctttag tgacctttta gggtttgctt ttattttcct
     5701 ctcttttttg tattatttct ttcaaacata gtaaatatat gattttacag ccaaagtggt
     5761 attctgtttt ttctaagctt tgagatacaa atattgctat ttaatcacat tgatgatgaa
     5821 ttactttcca tgtgaacctt ctcaaaatga tttaagtacc tttgattttg ctcccctaaa
     5881 aatggcttgt gtctaaagtg acattttttt ccctatttgg agggttctaa tgaaagagat
     5941 ccaggcataa aaactctttt gcttattgaa agcaatttga ttacacctat gtttcaggtt
     6001 atagtgggga gaaaatttgg tcagtgcctt attatttgaa gagcaatttt agggcatcaa
     6061 gtatgacaat aatcatgtgc cctcttgctc ctcttatttc aggagtgtgt gctatccctc
     6121 tctccaccag cttaatcttt ctcaccccaa gtctaacaaa gtctttacag aaacctctgt
     6181 aggtgattgt tgcagtcttc cttggttttt gagtcttttt aaacaagctc ctaccctgct
     6241 tctatccttt tagagtagga gattaaaaat aaacccaccc cagtcaacct ttagataagc
     6301 actcttaaaa acaaatttga agagcactct tcagccaagt tcatttttat tttgttgttg
     6361 atgttttgag catacattta attgtaaggc cttttggaaa agccctaaaa gaataagata
     6421 caaccagaaa tatttgagtt ttgttaaatg tactgaaatc ttgtaatgaa aatccctttg
     6481 gccagaaatg agatttattt ccatttattt ttacatttaa gtaacactca aaacaaacaa
     6541 gtagtcactt aacctgccag ttattatttt cttgaaaata tacaagaaag taaaaatatc
     6601 tctaatatat aagttaaagg aaagttaaga gactaagcta gttttttcag tgaaagcaaa
     6661 gttggaaata ttttgcttct ctcagattat tggaagacct agagctactg gatgttaacc
     6721 tgaatcaaga tgttcttttc acattttttt aaagggctag tatgttccag agtaggcaag
     6781 tagtcattca agccaaatga aagagttaac tggtccttga ggagtgttag caaaatttaa
     6841 attttgaccc tggccatttg tactacagaa gtgattatgg gattaaaaga atacataatt
     6901 acagtgtttt gggattgggc tctttttttt cttaatagaa aagcagaaac ttcataaata
     6961 atagctgtgc tttagatacc agataacaaa tattgtttcc cctgaagata tgacctacta
     7021 gaactactca catatatagt ccaataatta ctgacttaat aggtatggta aaatagctga
     7081 taataagtca gactctcaag agtttctgta ccttgattat tgacaaattc attgttttac
     7141 atcctactaa agaacatgtg tgtggggagg gggtggggaa ctggttcaca acataatctg
     7201 aaggagatca aacatctgta aggacaggta cccagtgatg ataatatatc tgaaaacaca
     7261 agccattttt attctttatc ccaattaact tgaggtactc taatgatgaa gcactcgatt
     7321 gcactatgac ctccttgagt gatgtgcagc ttggttcctc tctcactttt tgtttctttt
     7381 taatatgcaa atgtgagtgt gcgatcttca gtgtgtctgc ataagctaac ttaagatgaa
     7441 tttaagtaca gttttctgaa atatttctat tgaaataaat tacttaaaat ta
//
