LOCUS       NM_014636               6330 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens Ral GEF with PH domain and SH3 binding motif 1
            (RALGPS1), transcript variant 1, mRNA.
ACCESSION   NM_014636
VERSION     NM_014636.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Guo,H., Wang,S., Xie,A., Sun,W., Wei,C., Xian,S., Yin,H., Li,M.,
            Sun,H., Li,H., Meng,T., Zhang,J. and Huang,Z.
  TITLE     Ral GEF with the PH Domain and SH3 Binding Motif 1 Regulated by
            Splicing Factor Junction Plakoglobin and Pyrimidine Metabolism Are
            Prognostic in Uterine Carcinosarcoma
  JOURNAL   Dis Markers 2021, 1484227 (2021)
   PUBMED   34745385
  REMARK    GeneRIF: Ral GEF with the PH Domain and SH3 Binding Motif 1
            Regulated by Splicing Factor Junction Plakoglobin and Pyrimidine
            Metabolism Are Prognostic in Uterine Carcinosarcoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 6330)
  AUTHORS   Peng,W., Xu,J., Guan,X., Sun,Y., Zhang,X.C., Li,X. and Rao,Z.
  TITLE     Structural study of the Cdc25 domain from Ral-specific
            guanine-nucleotide exchange factor RalGPS1a
  JOURNAL   Protein Cell 2 (4), 308-319 (2011)
   PUBMED   21494904
  REMARK    GeneRIF: The guanine-nucleotide exchange factor (GEF) RalGPS1a
            activates small GTPase Ral proteins such as RalA and RalB by
            stimulating the exchange of Ral bound GDP to GTP, thus regulating
            various downstream cellular processes.
REFERENCE   4  (bases 1 to 6330)
  AUTHORS   Choy,K.W., Wang,C.C., Ogura,A., Lau,T.K., Rogers,M.S., Ikeo,K.,
            Gojobori,T., Lam,D.S. and Pang,C.P.
  TITLE     Genomic annotation of 15,809 ESTs identified from pooled early
            gestation human eyes
  JOURNAL   Physiol Genomics 25 (1), 9-15 (2006)
   PUBMED   16368877
REFERENCE   5  (bases 1 to 6330)
  AUTHORS   de Bruyn,K.M., de Rooij,J., Wolthuis,R.M., Rehmann,H.,
            Wesenbeek,J., Cool,R.H., Wittinghofer,A.H. and Bos,J.L.
  TITLE     RalGEF2, a pleckstrin homology domain containing guanine nucleotide
            exchange factor for Ral
  JOURNAL   J Biol Chem 275 (38), 29761-29766 (2000)
   PUBMED   10889189
REFERENCE   6  (bases 1 to 6330)
  AUTHORS   Rebhun,J.F., Chen,H. and Quilliam,L.A.
  TITLE     Identification and characterization of a new family of guanine
            nucleotide exchange factors for the ras-related GTPase Ral
  JOURNAL   J Biol Chem 275 (18), 13406-13410 (2000)
   PUBMED   10747847
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AB002349.1 and CN266562.1.
            
            On Nov 22, 2018 this sequence version replaced NM_014636.2.
            
            Transcript Variant: This variant (1) encodes isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB002349.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN03465402, SAMN03465404
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000259351.10/ ENSP00000259351.5
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5992              AB002349.1         9-6000
            5993-6330           CN266562.1         174-511
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q33.3"
     gene            1..6330
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /note="Ral GEF with PH domain and SH3 binding motif 1"
                     /db_xref="GeneID:9649"
                     /db_xref="HGNC:HGNC:16851"
                     /db_xref="MIM:614444"
     exon            1..194
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            195..316
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     CDS             260..1933
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /note="isoform 1 is encoded by transcript variant 1; Ral
                     guanine nucleotide exchange factor RalGPS1A; ras-specific
                     guanine nucleotide-releasing factor RalGPS1; ralA exchange
                     factor RalGPS1; ral guanine nucleotide exchange factor 2"
                     /codon_start=1
                     /product="ras-specific guanine nucleotide-releasing factor
                     RalGPS1 isoform 1"
                     /protein_id="NP_055451.1"
                     /db_xref="CCDS:CCDS35143.1"
                     /db_xref="GeneID:9649"
                     /db_xref="HGNC:HGNC:16851"
                     /db_xref="MIM:614444"
                     /translation="MYKRNGLMASVLVTSATPQGSSSSDSLEGQSCDYASKSYDAVVF
                     DVLKVTPEEFASQITLMDIPVFKAIQPEELASCGWSKKEKHSLAPNVVAFTRRFNQVS
                     FWVVREILTAQTLKIRAEILSHFVKIAKKLLELNNLHSLMSVVSALQSAPIFRLTKTW
                     ALLNRKDKTTFEKLDYLMSKEDNYKRTREYIRSLKMVPSIPYLGIYLLDLIYIDSAYP
                     ASGSIMENEQRSNQMNNILRIIADLQVSCSYDHLTTLPHVQKYLKSVRYIEELQKFVE
                     DDNYKLSLRIEPGSSSPRLVSSKEDLAGPSAGSGSARFSRRPTCPDTSVAGSLPTPPV
                     PRHRKSHSLGNNMMCQLSVVESKSATFPSEKARHLLDDSVLESRSPRRGLALTSSSAV
                     TNGLSLGSSESSEFSEEMSSGLESPTGPCICSLGNSAAVPTMEGPLRRKTLLKEGRKP
                     ALSSWTRYWVILSGSTLLYYGAKSLRGTDRKHYKSTPGKKVSIVGWMVQLPDDPEHPD
                     IFQLNNPDKGNVYKFQTGSRFHAILWHKHLDDACKSNRPQVPANLMSFE"
     exon            317..424
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            425..475
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            476..559
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            560..649
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            650..742
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            743..869
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            870..1007
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1101
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1102..1169
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1296
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1297..1454
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1506
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1507..1612
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1613..1705
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1706..1811
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1812..1903
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     exon            1904..6330
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2617..2622
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /note="hexamer: AACAAA"
     polyA_site      2634
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /note="major polyA site"
     regulatory      6309..6314
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /note="hexamer: ATTAAA"
     polyA_site      6330
                     /gene="RALGPS1"
                     /gene_synonym="RALGEF2; RALGPS1A"
                     /note="major polyA site"
ORIGIN      
        1 aggccctgga gcggacggtt cctactgcgg ctgggcaccg gctccgctcc cgcgtctgcc
       61 cgcgctccag ctgcgcctgg cccggccccg gcccggctcg gcgtggcccc ggcctccaag
      121 cgaaggcgcc gctgccgctg ggccgctccc agggccatga ggaagcggcg gcagccactg
      181 cggcccgcgt caaggacttc tccagacagg ttatgttacc tgcagaggct gccctgaagc
      241 tccctgtggc ctggagacta tgtacaagag gaatggtctg atggctagcg tgttggtcac
      301 ctctgccact ccacagggca gcagcagctc ggactctctg gagggccaga gctgcgacta
      361 tgccagcaag agctatgatg ccgttgtctt cgatgtcttg aaagtgaccc cagaggagtt
      421 tgctagccag attacattaa tggatatacc tgtgtttaaa gctatccagc cggaggaact
      481 agccagctgt ggatggagta agaaggagaa acacagtctt gcccctaacg ttgtggcctt
      541 tacccggagg tttaaccagg tcagtttttg ggttgtacga gaaattctaa cagcacagac
      601 tttaaaaata agggcagaaa tcctcagcca ttttgtgaaa atagccaaga aacttctaga
      661 actcaacaac cttcattctc tcatgtctgt ggtatcagca ttacaaagtg ctcccatctt
      721 caggctgaca aaaacctggg ctcttttaaa tcgaaaagac aagactacct ttgagaaatt
      781 ggactacctg atgtcgaaag aagataatta caagcggaca cgggaatata tccgaagcct
      841 gaagatggtt ccaagtattc cctatctagg aatctatctt ctggatttaa tctacattga
      901 ttctgcatat cctgcctcag gcagtatcat ggaaaatgaa caaagatcca atcagatgaa
      961 caatattctt cgaataattg ctgatttaca agtttcctgc agctatgatc acctcaccac
     1021 cctgccccat gtgcagaagt acctgaagtc cgtacgctac attgaagagc tccagaagtt
     1081 tgtggaagac gacaactaca aactgtcgct cagaatcgaa ccaggaagca gctctccaag
     1141 actagtctct tccaaggaag atcttgcagg tccctctgct ggctccggtt ctgcgaggtt
     1201 cagccggagg cccacctgtc ctgacacatc tgttgctggc agcctcccca cacctccagt
     1261 ccccagacac aggaagagcc acagcctagg caacaatatg atgtgtcagt tgagtgtagt
     1321 tgagagtaaa agtgcgacat tcccatcgga gaaagcaagg cacctactgg acgacagtgt
     1381 cctagagtcc cgcagccccc gaaggggcct ggctctgacc tcctcctctg ctgtcaccaa
     1441 tggactctcc ctaggcagta gtgagagctc agagtttagt gaagagatgt cttcagggct
     1501 ggaaagcccc accggcccgt gcatctgttc tctggggaac tccgcagctg tgcccaccat
     1561 ggaggggcct ctgagaagaa aaaccctgct caaggaaggg cggaagcctg cgctgtcctc
     1621 gtggaccagg tactgggtca tactctcagg atccaccctc ctgtactacg gagccaagtc
     1681 cttgcggggc acagacagaa aacactataa atccacacct ggcaaaaagg tttccatcgt
     1741 gggctggatg gtgcagctgc ccgatgaccc cgagcaccca gatatcttcc agctgaacaa
     1801 ccctgacaaa ggcaatgttt acaagtttca gactggttcc cgatttcatg caatactgtg
     1861 gcacaagcat ttggatgatg catgtaaaag caacaggcct caggtacctg caaaccttat
     1921 gtcatttgag taagtctctg caggacgtgg catgacttca gaggcttctg ggaacccagg
     1981 ctgggcctgg tggtgaagag cagtcctggg cacaggctgt gagccagggt gctgggaaac
     2041 tcacagctgg actcagggga cacggcctgt ggcctcacca tcccagaggg cttcaccagt
     2101 gtgggatcca cctgtcagtc cccagcgact ctcatgacac tcattctgca gcaccgcctc
     2161 ttggggcagt ggtcagaccc cacacgccct ctctgggccc accacctgca tctgcgacta
     2221 gagagcaccc ggcccacgtt gggttctcag tgctttctac tgcacagagt ggacagcgct
     2281 aactaacctg tgagaggggc ccgagagaag gaacagctgt ggaacaggct ttttacaccc
     2341 caagtgcatg gggttgctcg cccacagggc tgcctcagat tttgtacaac cccgaagcgt
     2401 cctctgcgtg tgcgtgctgt acgtgtgtgt gtgtgtgtga gcgagtgtga actcttcaag
     2461 aaacatgcat tttggcacaa gactcgtgac atcacacact tcattcgctt tgaggccctg
     2521 ctttaacctt aagttatagc cctgtccacc gaggaaggtc agggtgagag cctagattcc
     2581 tcctgtgtca agggtccctc gcattctttt actgtaaaca aacaatgcct taaattgtgt
     2641 cttgttttct gttcctatgg gtgctattca tctggaaggc ctgcttccag gcctctttgc
     2701 tgtcagccct tctgagacag gacctggctt caggactgtg gactgggctg ctggcctgct
     2761 tgcttcctcc cttccccatt cctagcaggg cctgaggccc tcctcttctc gcccttccca
     2821 ccatgccaga atgggaagtt gtgacgttgc agctccaacc gacgtgctca tagtgatcag
     2881 ctgtgcagga gccatgaggc accaacctct ccccgcaggg caaagcctgt gcccccatca
     2941 tctcactcct ttgcctgcac tgccagggtg gggcccacca agattcctga tcatgacggg
     3001 aagctgagtg accctgaggc cttaagcttc cccagtcttg gccccaaatg cagtcaccag
     3061 caagttttcc attttccaag tccaagggca caattgttga tgaccgtgtg acaatagagc
     3121 gaagccccgg ggagtgaacg gtccaacctc tgcattcagt taggagctct tcacatgaat
     3181 cacatcctta tctgtcacct tgtgtcacat tttaaagtga cttttatttt gcacaaataa
     3241 tttttattca gaataataaa tcactcttta tcatagtatc ttctcttccc tcttcccctt
     3301 tagtttggat agcctaactc tgagaagtta acccttaaac agttttctgg aagagactga
     3361 atttctgggt ccttgcagct gtgatggttt cagagctcag actgatcagg catcaagcta
     3421 ccctcaagag tttctgggct ggatgtttca gaacaacatc tacaccagta aagtgtaata
     3481 ggtcagtttc aaaacgacca aaagacccac cactgtattt tgaccaaata atgacaactt
     3541 ctttagaaat ttgaatggct tggtgaggaa agtagttgtc accagggcct cattttgtag
     3601 ttgagcctta caatgcttag tagttcatct tctttttgag caaagactag aatactttcc
     3661 tcctaagaga aactcccagg tgataaaagt tgatgccatc aaaccttgac accgggtgct
     3721 ctgcacaccc acgcggatgt tgcacctcat tctcccgatg actattcaaa tcagcatcta
     3781 gaggctgaat gacaatgcca aacactccac ctctgatcag aaccatgcag tgttaacact
     3841 ttaacctaca ttgaatctga ttctacctgt taacttttaa aaagtcgtaa gtttggatga
     3901 aagtgcaaga tgtggaacat caactaccta ttttccttgg gtttttccac tctgcaaact
     3961 gtcctggttt ttcacaccaa tgaagtatta tagatgccaa tccaaaacct cagaatttca
     4021 ggcaccacaa aaacaggtaa ttttctatcc cttataagtt tgtcttttct ttcagaaaca
     4081 tctcttagcc taatttgaaa tagcacaatc acaattcaaa atgtttagtc ttctcactaa
     4141 ttgagtctgc ttccacgtcc tctcccagga acattcttag ctcggactct tgaagaatct
     4201 ctttagattt tgttggcaaa agccttatag aagcagtaag aggcttgacc acgccggaag
     4261 agtcctggag ctaaagctgg aagacactca gctctctaag caggggctcg gccaaacatg
     4321 ggagttaagt gctgcttgtc ttcccagtgt tggtttgaac cctgtgagcc tgagacagag
     4381 agggccaggc accaaccaca aggcgggaaa gtccatgggt agaccctccc cctggaggga
     4441 agcatttcta gtttttgctc cttgactgtc cagagtgtac aaatgttcat aacgccattg
     4501 aagggattat ttcttgcatg catatgctga atttttttaa gcaaatggat catggcaccc
     4561 caaaatgaaa gttatagaaa gctgtctaca actgtggagt tggtagctgg taacattgtt
     4621 gtctcaagaa caactcacct ctctccctag gactaatttt tgtctctctc agttgaacat
     4681 gttttgtcat tcaagatcag tcaggtgcat tctggcaact gacatacttg atggaggatt
     4741 gattcggtag agagcagtag aaatcttgtt ctaactgtgc ctggtgagag actttggccc
     4801 cctccctccc tataaggctg tggaacctga ggaagtagat acttgaagag attctgttta
     4861 ggaagaaact cactctcttt tgccagttga atttatagag catttttttt cttaccaaga
     4921 tggccagtat cattttaccc ccacctccca agccccaaga ggtgtacctt ttcagatgcc
     4981 attttacagg cggaaatgct ccatgaaaca ggaagccact tgcaagcaac atctgctctg
     5041 ttcctcaggt ggggcccaga gcccttcccc gagactgctg atgtctgtaa ccactgggga
     5101 gcactgccaa aaatacagct ttctggtttg tgagcccata aatgacttaa atcagcttta
     5161 catcattttt acatatcaag tggtttcatg ttaaaaaaca aactcctagt cctttagaaa
     5221 taacagattc tctgcacaaa accacccatt cattcattta ttcattcaca gcactagcaa
     5281 gtgctgccta tgctgagaac aagtcagatc tgatccctgc cctcatggac ctgaccactc
     5341 aacaaacagt ccccaccaca cctatctcct taggcaagac tttgcctctc tcctagtcct
     5401 gagtataaat cctgtgcata gattcctcta gaaaggcatc aaaaggctca acagactgaa
     5461 tggcctcttg gtctgcgaaa attcagttgc aatgaggatg aagtcactat cctagaggct
     5521 gcttggccca gaagagccag gcacagagct gcagttgggc acgccaagga ttccaaaggt
     5581 ggaatgagag agtagggtca aactgtcaca gtatctgctc cataggtttc tgtttttaat
     5641 ttcaatgtta aatacaacta caatatgagc gagaactgca ttttcttggg tgttgagaac
     5701 ttgtaccatg gacttcagac cgccttgcag ccgtatgctg cacaagcgtg tacaccccct
     5761 gggcagcctc aaaaccccgc ttacagcagc aacacaggag atcatctgtc cattttagaa
     5821 ccattaatct ctttatccat tgctgaacga ctgtgactat tcagtaacga agtaatagta
     5881 attaattagt atggtataat ctttaataaa tttcgtgcca aaatgcatgg ttttccactt
     5941 agcattcaaa atgttgcata gagagtagtt ttcaatttct tatgtactct tcgaagtaag
     6001 ttgaaaatca gtttctacat tttaattcgt ttcctgttaa atctgttgca ctctcctggg
     6061 ctgtcttttt ctccagcaga cccctgcatg cagttgtgta aggactttct ctaattcttg
     6121 tgaatcgtct cacccgcagt aaccactgaa cgtcaatcag ccctccatgg ggttctttcg
     6181 atttttggtg aagtattttg ttacctcagt cttgtatcaa gttgctgtat ttttcagctt
     6241 gttacattga taataattat ttcactaatt aaatacttta atgtacaaac atctttgttt
     6301 actttgaaat taaatgtgtt ttccaatgaa
//
