LOCUS       NM_006266               3696 bp    mRNA    linear   PRI 03-OCT-2024
DEFINITION  Homo sapiens ral guanine nucleotide dissociation stimulator
            (RALGDS), transcript variant 1, mRNA.
ACCESSION   NM_006266
VERSION     NM_006266.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Chen,T.C., Lin,K.T., Chen,C.H., Lee,S.A., Lee,P.Y., Liu,Y.W.,
            Kuo,Y.L., Wang,F.S., Lai,J.M. and Huang,C.Y.
  TITLE     Using an in situ proximity ligation assay to systematically profile
            endogenous protein-protein interactions in a pathway network
  JOURNAL   J Proteome Res 13 (12), 5339-5346 (2014)
   PUBMED   25241761
REFERENCE   3  (bases 1 to 3696)
  AUTHORS   Smith,M.J. and Ikura,M.
  TITLE     Integrated RAS signaling defined by parallel NMR detection of
            effectors and regulators
  JOURNAL   Nat Chem Biol 10 (3), 223-230 (2014)
   PUBMED   24441586
REFERENCE   4  (bases 1 to 3696)
  AUTHORS   Ragain,C.M., Newberry,R.W., Ritchie,A.W. and Webb,L.J.
  TITLE     Role of electrostatics in differential binding of RalGDS to Rap
            mutations E30D and K31E investigated by vibrational spectroscopy of
            thiocyanate probes
  JOURNAL   J Phys Chem B 116 (31), 9326-9336 (2012)
   PUBMED   22738401
  REMARK    GeneRIF: Investigated is the role that electrostatic field
            differences caused by the charge reversal mutation Rap1A K31E play
            in determining the binding specificity of RalGDS to Rap1A versus
            Ras.
REFERENCE   5  (bases 1 to 3696)
  AUTHORS   Gloerich,M., ten Klooster,J.P., Vliem,M.J., Koorman,T.,
            Zwartkruis,F.J., Clevers,H. and Bos,J.L.
  TITLE     Rap2A links intestinal cell polarity to brush border formation
  JOURNAL   Nat Cell Biol 14 (8), 793-801 (2012)
   PUBMED   22797597
REFERENCE   6  (bases 1 to 3696)
  AUTHORS   Miller,M.J., Prigent,S., Kupperman,E., Rioux,L., Park,S.H.,
            Feramisco,J.R., White,M.A., Rutkowski,J.L. and Meinkoth,J.L.
  TITLE     RalGDS functions in Ras- and cAMP-mediated growth stimulation
  JOURNAL   J Biol Chem 272 (9), 5600-5605 (1997)
   PUBMED   9038168
REFERENCE   7  (bases 1 to 3696)
  AUTHORS   Urano,T., Emkey,R. and Feig,L.A.
  TITLE     Ral-GTPases mediate a distinct downstream signaling pathway from
            Ras that facilitates cellular transformation
  JOURNAL   EMBO J 15 (4), 810-816 (1996)
   PUBMED   8631302
REFERENCE   8  (bases 1 to 3696)
  AUTHORS   Spaargaren,M. and Bischoff,J.R.
  TITLE     Identification of the guanine nucleotide dissociation stimulator
            for Ral as a putative effector molecule of R-ras, H-ras, K-ras, and
            Rap
  JOURNAL   Proc Natl Acad Sci U S A 91 (26), 12609-12613 (1994)
   PUBMED   7809086
REFERENCE   9  (bases 1 to 3696)
  AUTHORS   Hofer,F., Fields,S., Schneider,C. and Martin,G.S.
  TITLE     Activated Ras interacts with the Ral guanine nucleotide
            dissociation stimulator
  JOURNAL   Proc Natl Acad Sci U S A 91 (23), 11089-11093 (1994)
   PUBMED   7972015
REFERENCE   10 (bases 1 to 3696)
  AUTHORS   Albright,C.F., Giddings,B.W., Liu,J., Vito,M. and Weinberg,R.A.
  TITLE     Characterization of a guanine nucleotide dissociation stimulator
            for a ras-related GTPase
  JOURNAL   EMBO J 12 (1), 339-347 (1993)
   PUBMED   8094051
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DR003030.1, DB480866.1, AF295773.1, BC059362.1 and BC033198.1.
            
            On Nov 22, 2018 this sequence version replaced NM_006266.3.
            
            Summary: Guanine nucleotide dissociation stimulators (GDSs, or
            exchange factors), such as RALGDS, are effectors of Ras-related
            GTPases (see MIM 190020) that participate in signaling for a
            variety of cellular processes.[supplied by OMIM, Nov 2010].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.214760.1,
                                           SRR1803614.101243.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000372050.8/ ENSP00000361120.3
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-43                DR003030.1         18-60
            44-461              DB480866.1         1-418
            462-2704            AF295773.1         387-2629
            2705-3096           BC059362.1         2669-3060
            3097-3696           BC033198.1         1503-2102
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.13-q34.2"
     gene            1..3696
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /note="ral guanine nucleotide dissociation stimulator"
                     /db_xref="GeneID:5900"
                     /db_xref="HGNC:HGNC:9842"
                     /db_xref="MIM:601619"
     exon            1..269
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /inference="alignment:Splign:2.1.0"
     CDS             87..2831
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /note="isoform 1 is encoded by transcript variant 1; ral
                     guanine nucleotide exchange factor"
                     /codon_start=1
                     /product="ral guanine nucleotide dissociation stimulator
                     isoform 1"
                     /protein_id="NP_006257.1"
                     /db_xref="CCDS:CCDS6959.1"
                     /db_xref="GeneID:5900"
                     /db_xref="HGNC:HGNC:9842"
                     /db_xref="MIM:601619"
                     /translation="MVQRMWAEAAGPAGGAEPLFPGSRRSRSVWDAVRLEVGVPDSCP
                     VVLHSFTQLDPDLPRPESSTQEIGEELINGVIYSISLRKVQLHHGGNKGQRWLGYENE
                     SALNLYETCKVRTVKAGTLEKLVEHLVPAFQGSDLSYVTIFLCTYRAFTTTQQVLDLL
                     FKRYGRCDALTASSRYGCILPYSDEDGGPQDQLKNAISSILGTWLDQYSEDFCQPPDF
                     PCLKQLVAYVQLNMPGSDLERRAHLLLAQLEHSEPIEAEPEALSPVPALKPTPELELA
                     LTPARAPSPVPAPAPEPEPAPTPAPGSELEVAPAPAPELQQAPEPAVGLESAPAPALE
                     LEPAPEQDPAPSQTLELEPAPAPVPSLQPSWPSPVVAENGLSEEKPHLLVFPPDLVAE
                     QFTLMDAELFKKVVPYHCLGSIWSQRDKKGKEHLAPTIRATVTQFNSVANCVITTCLG
                     NRSTKAPDRARVVEHWIEVARECRILKNFSSLYAILSALQSNSIHRLKKTWEDVSRDS
                     FRIFQKLSEIFSDENNYSLSRELLIKEGTSKFATLEMNPKRAQKRPKETGIIQGTVPY
                     LGTFLTDLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKLLQSACNNYSIAPDEQFG
                     AWFRAVERLSETESYNLSCELEPPSESASNTLRTKKNTAIVKRWSDRQAPSTELSTSG
                     SSHSKSCDQLRCGPYLSSGDIADALSVHSAGSSSSDVEEINISFVPESPDGQEKKFWE
                     SASQSSPETSGISSASSSTSSSSASTTPVAATRTHKRSVSGLCNSSSALPLYNQQVGD
                     CCIIRVSLDVDNGNMYKSILVTSQDKAPAVIRKAMDKHNLEEEEPEDYELLQILSDDR
                     KLKIPENANVFYAMNSTANYDFVLKKRTFTKGVKVKHGASSTLPRMKQKGLKIAKGIF
                     "
     exon            270..380
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /inference="alignment:Splign:2.1.0"
     exon            381..574
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /inference="alignment:Splign:2.1.0"
     exon            575..670
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /inference="alignment:Splign:2.1.0"
     exon            671..864
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /inference="alignment:Splign:2.1.0"
     exon            865..1283
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1499
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..1603
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..1688
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /inference="alignment:Splign:2.1.0"
     exon            1689..1757
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..1844
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /inference="alignment:Splign:2.1.0"
     exon            1845..1877
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /inference="alignment:Splign:2.1.0"
     exon            1878..1999
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /inference="alignment:Splign:2.1.0"
     exon            2000..2095
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /inference="alignment:Splign:2.1.0"
     exon            2096..2297
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /inference="alignment:Splign:2.1.0"
     exon            2298..2540
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /inference="alignment:Splign:2.1.0"
     exon            2541..2655
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /inference="alignment:Splign:2.1.0"
     exon            2656..3696
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3676..3681
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /note="hexamer: ATTAAA"
     polyA_site      3696
                     /gene="RALGDS"
                     /gene_synonym="RalGEF; RGDS; RGF"
                     /note="major polyA site"
ORIGIN      
        1 agcggtggca aagcccagcc gcgcgggccg cgccgccgcc cccgccgcgc gccgggccgg
       61 ccccgcgccc gcgctgcgag ccttccatgg tgcagcgcat gtgggccgag gcggccgggc
      121 ctgctggcgg cgccgagccg ctgtttccgg gctcccggcg gagccgcagc gtgtgggacg
      181 ccgtgcgcct ggaggtgggc gtccccgaca gctgcccggt ggtgctgcac agcttcacgc
      241 agctagaccc cgacctgccg cgcccggaga gctccacgca ggagatcggt gaggagctga
      301 tcaacggagt catctactcc atctccctgc gcaaggtgca gctgcaccac ggaggcaaca
      361 aggggcagcg ctggctcggg tatgagaatg agtcggccct gaacctttat gagacttgca
      421 aggtgcggac cgtgaaggct ggcacgctgg agaagctggt ggagcacctg gtgccagcct
      481 tccagggcag cgacctctcc tacgtcacca tcttcctgtg tacctataga gccttcacca
      541 ccacccaaca ggtcctggac ctgctgttca aaaggtacgg tagatgtgac gccctcacgg
      601 cctcctctag atacggctgc atcctcccct attccgacga ggatggtgga ccccaggacc
      661 aacttaaaaa tgccatctcc tccatcctgg gcacctggct ggaccagtac tcggaggatt
      721 tctgtcaacc tccggacttt ccctgcctca agcagctggt ggcctacgtg cagctcaaca
      781 tgccaggctc agacctggag cgccgtgccc accttctcct ggcccagctg gagcactcgg
      841 aacccattga ggcagagcct gaggctctgt caccagtgcc agctctaaaa ccaactccag
      901 agctcgagct agctctaaca ccagctcgag cacccagccc agtgccggct ccagccccgg
      961 agccagagcc agctccaaca ccagctccag gttcagagct agaagtagct ccagcaccag
     1021 ctccggagct ccagcaggct ccagagccag ctgtgggact agaatcggct ccagcgccag
     1081 ctctggaact agagccagct ccagaacagg atccagctcc ctcacaaact ctagagctgg
     1141 agccagctcc agcaccagtt ccatcattac agccttcctg gccttcacct gtggttgcag
     1201 agaacgggct gagtgaggag aagcctcacc tcttggtgtt ccctccagat ctggtggcag
     1261 agcagtttac actgatggat gcggaactgt tcaagaaggt ggtgccctac cactgcctgg
     1321 gctccatctg gtcccagcgg gacaagaagg gcaaggagca cctggcgccc accatccgcg
     1381 ccactgtcac ccagttcaac agtgtggcca actgtgtcat caccacctgc ctcgggaacc
     1441 gaagcacgaa agccccagac agggccaggg tggtggagca ctggatcgag gtggccaggg
     1501 agtgccggat cctcaagaac ttctcgtcac tgtatgccat cctctctgcc ctgcagagca
     1561 actccatcca ccgtctgaag aagacgtggg aagacgtttc cagggacagt ttccggatct
     1621 ttcagaagct gtcagagatc ttctcagatg agaacaacta ctcattgagc cgggagctgc
     1681 tcatcaagga gggcacctcc aagtttgcca ccctggagat gaaccccaag agagcccaga
     1741 aacggccgaa ggagacgggc atcatccagg gcaccgttcc ctacctgggc acgttcctca
     1801 ccgacctggt gatgctggac actgccatga aggactatct gtatggcaga ctcatcaact
     1861 ttgagaagag gaggaaggag ttcgaggtga tcgcccagat caagctgctg cagtcggcct
     1921 gcaacaacta cagcatcgcg ccagatgagc aatttggggc ctggttccgg gccgtggagc
     1981 ggctcagcga gactgagagc tacaacctgt cgtgcgagct ggagccccca tccgagtcag
     2041 ccagcaacac cctcaggacc aagaagaaca cagccattgt caagcgctgg agcgaccgcc
     2101 aggcccccag cactgagctc agtaccagtg gcagctccca ctccaagtcc tgtgaccagc
     2161 tcaggtgtgg cccctacctc agcagcgggg acatcgctga cgcgctcagc gtgcactcgg
     2221 ccggctcctc tagctccgac gtggaggaga tcaacatcag cttcgtcccg gagtctcctg
     2281 atggccagga aaagaagttc tgggaatcag cctcacagtc atccccggag acctccggca
     2341 tcagctcagc ctccagcagc acctcgtcct cctcagcctc caccacgccc gtggctgcca
     2401 cacgcaccca caagcgctct gtctcagggc tctgcaactc cagctccgcg ctgccgctct
     2461 acaaccagca ggtgggcgac tgctgtatca tccgcgtcag cctggacgtg gacaatggca
     2521 acatgtacaa gagcatcctg gtgaccagcc aagataaggc tccggctgta atccgcaagg
     2581 ccatggacaa acacaacctg gaggaggagg agccggagga ctatgagctg ctgcagattc
     2641 tctcagatga ccggaagctg aagatccctg aaaacgccaa cgtcttctat gccatgaact
     2701 ctaccgccaa ctatgacttt gtcctcaaga agcggacctt caccaaggga gtgaaggtca
     2761 agcacggagc cagctccacc ctccctcgca tgaagcagaa aggactcaag attgccaagg
     2821 gcatcttctg agggcatcct cccagggtct ggctggctgg tagccaagca cttatggacc
     2881 agagtggccc aggccagctg ggcgccttcc tcccacctgc cagcccaggg taccccagac
     2941 tccagtttca tcctgaacct ctcccgctgc tgggattgac gcctgccatt ggtcaggctg
     3001 acctggcctc ccgtggacca ctcgctgcct taggtgcctt ctgctctctg gaaccagagg
     3061 actagctgac ttttgccaag gagcagtgcc aacgggcatg gcatggtgcc ctgcctgccc
     3121 ccgggcgcca cctctgtaca cttccctgac accttcccag gtgtgggtca ctgccacctg
     3181 tgcccatggg caccccagag cacccactgt gaccactgca gttctctcat gcccacaggc
     3241 actggcctgt gaccttcgca ggggtcccgg cccctcccac cactctagcc tttctcaggc
     3301 tgcaccaaag attccatcat cagggccaac tgagagtgag ggagtctcac ccaccgctta
     3361 ccccagccct cccctgggag cagagagaga aaccctcttc atggaccaga ctctgcaccc
     3421 ggtgagtgag gacagtccca gctgagtccc atcgatgttg aatctcatgc cactgcaagt
     3481 gccattcacc actgcgtcct gggctttacg agaccatgca agacgggggt tagtgaggaa
     3541 ggaggatttg gggtgggggt ggggtgattg aatatttgta taaaaagcaa aaagaaaaaa
     3601 aaaatgtttg tttactgatt ggggaggggc aatatttatt tgttgtaaat agcaaatgct
     3661 agacttgaat attatattaa aatcctgttt ctacta
//
