LOCUS       NM_020336               8633 bp    mRNA    linear   PRI 03-OCT-2024
DEFINITION  Homo sapiens Ral GTPase activating protein non-catalytic subunit
            beta (RALGAPB), transcript variant 1, mRNA.
ACCESSION   NM_020336 XM_028835
VERSION     NM_020336.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Yoshimachi,S., Shirakawa,R., Cao,M., Trinh,D.A., Gao,P., Sakata,N.,
            Miyazaki,K., Goto,K., Miura,T., Ariake,K., Maeda,S., Masuda,K.,
            Ishida,M., Ohtsuka,H., Unno,M. and Horiuchi,H.
  TITLE     Ral GTPase-activating protein regulates the malignancy of
            pancreatic ductal adenocarcinoma
  JOURNAL   Cancer Sci 112 (8), 3064-3073 (2021)
   PUBMED   34009715
  REMARK    GeneRIF: Ral GTPase-activating protein regulates the malignancy of
            pancreatic ductal adenocarcinoma.
REFERENCE   2  (bases 1 to 8633)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 8633)
  AUTHORS   Personnic,N., Lakisic,G., Gouin,E., Rousseau,A., Gautreau,A.,
            Cossart,P. and Bierne,H.
  TITLE     A role for Ral GTPase-activating protein subunit beta in mitotic
            regulation
  JOURNAL   FEBS J 281 (13), 2977-2989 (2014)
   PUBMED   24814574
  REMARK    GeneRIF: Deregulation of RalGAPbeta might cause genomic
            instability, leading to human carcinogenesis.
REFERENCE   4  (bases 1 to 8633)
  AUTHORS   Oshikawa,M., Tsutsui,C., Ikegami,T., Fuchida,Y., Matsubara,M.,
            Toyama,S., Usami,R., Ohtoko,K. and Kato,S.
  TITLE     Full-length transcriptome analysis of human retina-derived cell
            lines ARPE-19 and Y79 using the vector-capping method
  JOURNAL   Invest Ophthalmol Vis Sci 52 (9), 6662-6670 (2011)
   PUBMED   21697133
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8633)
  AUTHORS   Shirakawa,R., Fukai,S., Kawato,M., Higashi,T., Kondo,H., Ikeda,T.,
            Nakayama,E., Okawa,K., Nureki,O., Kimura,T., Kita,T. and
            Horiuchi,H.
  TITLE     Tuberous sclerosis tumor suppressor complex-like complexes act as
            GTPase-activating proteins for Ral GTPases
  JOURNAL   J Biol Chem 284 (32), 21580-21588 (2009)
   PUBMED   19520869
REFERENCE   6  (bases 1 to 8633)
  AUTHORS   Wu,C., Ma,M.H., Brown,K.R., Geisler,M., Li,L., Tzeng,E., Jia,C.Y.,
            Jurisica,I. and Li,S.S.
  TITLE     Systematic identification of SH3 domain-mediated human
            protein-protein interactions by peptide array target screening
  JOURNAL   Proteomics 7 (11), 1775-1785 (2007)
   PUBMED   17474147
REFERENCE   7  (bases 1 to 8633)
  AUTHORS   Gridley,S., Chavez,J.A., Lane,W.S. and Lienhard,G.E.
  TITLE     Adipocytes contain a novel complex similar to the tuberous
            sclerosis complex
  JOURNAL   Cell Signal 18 (10), 1626-1632 (2006)
   PUBMED   16490346
REFERENCE   8  (bases 1 to 8633)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
REFERENCE   9  (bases 1 to 8633)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL049868.20 and AL035419.12.
            
            On Nov 23, 2018 this sequence version replaced NM_020336.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1 and 2 encode the same isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC047482.1, SRR1803611.2929.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000262879.11/ ENSP00000262879.6
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-227               AL049868.20        29156-29382
            228-443             AL049868.20        44716-44931
            444-646             AL049868.20        49243-49445
            647-810             AL049868.20        53666-53829
            811-997             AL049868.20        55760-55946
            998-1129            AL049868.20        65390-65521
            1130-1308           AL049868.20        72505-72683
            1309-1457           AL049868.20        73819-73967
            1458-1674           AL049868.20        74097-74313
            1675-1876           AL049868.20        77810-78011
            1877-2044           AL049868.20        81091-81258
            2045-2159           AL049868.20        81717-81831
            2160-2307           AL049868.20        82208-82355
            2308-2372           AL049868.20        87480-87544
            2373-2502           AL049868.20        89043-89172
            2503-2636           AL049868.20        91387-91520
            2637-2819           AL049868.20        96089-96271
            2820-2971           AL049868.20        97354-97505
            2972-3159           AL049868.20        102556-102743
            3160-3266           AL049868.20        105002-105108
            3267-3419           AL049868.20        107384-107536
            3420-3629           AL049868.20        110180-110389
            3630-3788           AL049868.20        114608-114766
            3789-3954           AL049868.20        118845-119010
            3955-4074           AL049868.20        121672-121791
            4075-4211           AL035419.12        1587-1723
            4212-4320           AL035419.12        4379-4487
            4321-4399           AL035419.12        5260-5338
            4400-4548           AL035419.12        8641-8789
            4549-8633           AL035419.12        9265-13349
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q11.23"
     gene            1..8633
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /note="Ral GTPase activating protein non-catalytic subunit
                     beta"
                     /db_xref="GeneID:57148"
                     /db_xref="HGNC:HGNC:29221"
                     /db_xref="MIM:618833"
     exon            1..227
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            228..443
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     CDS             258..4742
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /note="isoform 1 is encoded by transcript variant 1; Ral
                     GTPase activating protein, beta subunit (non-catalytic);
                     Ral GTPase activating protein non-catalytic beta subunit"
                     /codon_start=1
                     /product="ral GTPase-activating protein subunit beta
                     isoform 1"
                     /protein_id="NP_065069.1"
                     /db_xref="CCDS:CCDS13305.1"
                     /db_xref="GeneID:57148"
                     /db_xref="HGNC:HGNC:29221"
                     /db_xref="MIM:618833"
                     /translation="MYSEWRSLHLVIQNDQGHTSVLHSYPESVGREVANAVVRPLGQV
                     LGTPSVAGSENLLKTDKEVKWTMEVICYGLTLPLDGETVKYCVDVYTDWIMALVLPKD
                     SIPLPVIKEPNQYVQTILKHLQNLFVPRQEQGSSQIRLCLQVLRAIQKLARESSLMAR
                     ETWEVLLLFLLQINDILLAPPTVQGGIAENLAEKLIGVLFEVWLLACTRCFPTPPYWK
                     TAKEMVANWRHHPAVVEQWSKVICALTSRLLRFTYGPSFPAFKVPDEDASLIPPEMDN
                     ECVAQTWFRFLHMLSNPVDLSNPAIISSTPKFQEQFLNVSGMPQELNQYPCLKHLPQI
                     FFRAMRGISCLVDAFLGISRPRSDSAPPTPVNRLSMPQSAAVSTTPPHNRRHRAVTVN
                     KATMKTSTVSTAHASKVQHQTSSTSPLSSPNQTSSEPRPLPAPRRPKVNSILNLFGSW
                     LFDAAFVHCKLHNGINRDSSMTAITTQASMEFRRKGSQMSTDTMVSNPMFDASEFPDN
                     YEAGRAEACGTLCRIFCSKKTGEEILPAYLSRFYMLLIQGLQINDYVCHPVLASVILN
                     SPPLFCCDLKGIDVVVPYFISALETILPDRELSKFKSYVNPTELRRSSINILLSLLPL
                     PHHFGTVKSEVVLEGKFSNDDSSSYDKPITFLSLKLRLVNILIGALQTETDPNNTQMI
                     LGAMLNIVQDSALLEAIGCQMEMGGGENNLKSHSRTNSGISSASGGSTEPTTPDSERP
                     AQALLRDYALNTDSAAGLLIRSIHLVTQRLNSQWRQDMSISLAALELLSGLAKVKVMV
                     DSGDRKRAISSVCTYIVYQCSRPAPLHSRDLHSMIVAAFQCLCVWLTEHPDMLDEKDC
                     LKEVLEIVELGISGSKSKNNEQEVKYKGDKEPNPASMRVKDAAEATLTCIMQLLGAFP
                     SPSGPASPCSLVNETTLIKYSRLPTINKHSFRYFVLDNSVILAMLEQPLGNEQNDFFP
                     SVTVLVRGMSGRLAWAQQLCLLPRGAKANQKLFVPEPRPVPKNDVGFKYSVKHRPFPE
                     EVDKIPFVKADLSIPDLHEIVTEELEERHEKLRSGMAQQIAYEIHLEQQSEEELQKRS
                     FPDPVTDCKPPPPAQEFQTARLFLSHFGFLSLEALKEPANSRLPPHLIALDSTIPGFF
                     DDIGYLDLLPCRPFDTVFIFYMKPGQKTNQEILKNVESSRTVQPHFLEFLLSLGWSVD
                     VGRHPGWTGHVSTSWSINCCDDGEGSQQEVISSEDIGASIFNGQKKVLYYADALTEIA
                     FVVPSPVESLTDSLESNISDQDSDSNMDLMPGILKQPSLTLELFPNHTDNLNSSQRLS
                     PSSRMRKLPQGRPVPPLGPETRVSVVWVERYDDIENFPLSELMTEISTGVETTANSST
                     SLRSTTLEKEVPVIFIHPLNTGLFRIKIQGATGKFNMVIPLVDGMIVSRRALGFLVRQ
                     TVINICRRKRLESDSYSPPHVRRKQKITDIVNKYRNKQLEPEFYTSLFQEVGLKNCSS
                     "
     exon            444..646
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            647..810
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            811..997
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            998..1129
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1308
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1309..1457
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1458..1674
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1675..1876
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            1877..2044
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2045..2159
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2307
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2308..2372
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2373..2502
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2503..2636
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2637..2819
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2820..2971
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            2972..3159
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            3160..3266
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            3267..3419
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            3420..3629
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            3630..3788
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            3789..3954
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            3955..4074
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            4075..4211
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            4212..4320
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            4321..4399
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            4400..4548
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     exon            4549..8633
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8446..8451
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /note="hexamer: AGTAAA"
     polyA_site      8470
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /note="major polyA site"
     regulatory      8606..8611
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /note="hexamer: ATTAAA"
     polyA_site      8633
                     /gene="RALGAPB"
                     /gene_synonym="KIAA1219; RalGAPbeta"
                     /note="major polyA site"
ORIGIN      
        1 agtcggaagt tgcctgagca gatcccagcc ggctggctcg agtggccttc gtcgtccctt
       61 ggcgccctgg gagagtcgct gacgggtgga ctgacggacc gcctgaggac ggccggccag
      121 ggcggtgaaa gcgccagccc tatggcgcgg gtcgcgtgag gcggaaggcc gaggacggcc
      181 ggcggcggcg cccgccccgg cgatgcgggc cccgcccgtc gcctcaggtg ccatttggat
      241 tgtactttag tggcacgatg tactctgagt ggaggtcact gcatttggtg attcagaatg
      301 atcaaggcca taccagtgtg ctgcacagct atccagagag cgttggacga gaggtggcaa
      361 atgctgtagt ccgtcctctt gggcaggtgt taggtacccc ttcagtggct ggtagtgaga
      421 atttgttaaa aactgacaaa gaagtaaaat ggaccatgga agtaatttgc tatggactga
      481 cccttccatt ggatggagag actgtaaaat attgcgttga tgtatataca gactggatta
      541 tggctttagt gttgccaaaa gattctattc cattgccagt tattaaagag cctaatcaat
      601 atgttcaaac tatactaaaa cacctacaga atctttttgt accaagacag gaacagggtt
      661 ccagtcagat tcgactatgc ttacaggtcc tgagagccat tcagaaactg gcccgtgagt
      721 catctctcat ggcccgagaa acttgggaag tcttactgtt gtttcttctg cagattaacg
      781 acatacttct ggccccacca actgttcaag gtggcattgc tgagaatcta gcagagaagt
      841 tgattggtgt tctctttgag gtgtggttac tagcttgtac tcggtgcttc ccaacacctc
      901 cttattggaa aacagccaag gagatggtgg ctaactggag gcatcaccca gcagtggtgg
      961 agcagtggag caaggtcatt tgtgcactca cttccagatt gctacgcttt acatatggtc
     1021 cttcatttcc tgcatttaaa gttcccgatg aagatgccag tctgatccct ccagaaatgg
     1081 ataatgagtg tgttgcacag acatggtttc gctttttaca catgttaagt aatcctgtgg
     1141 atttgagtaa cccagctatt ataagctcta ctcccaaatt tcaggaacag ttcttgaatg
     1201 tgagcggaat gccgcaagaa ttgaatcagt atccctgcct taaacatctg cctcaaatat
     1261 tttttcgtgc catgcgtgga atcagctgtc tggtggatgc attcttaggt atttctagac
     1321 cccgatcaga cagtgctccc ccaacacccg tgaatagatt aagtatgcct caaagtgctg
     1381 ctgtcagtac caccccccca cataaccgga ggcaccgggc tgttactgtg aataaggcca
     1441 ccatgaagac aagcacagtt agtactgctc atgcctctaa agttcagcac cagacgtcct
     1501 ccacctctcc tctgtcaagt ccaaatcaga ctagttcaga accccggcca ctgcctgccc
     1561 ctcggagacc aaaggttaac agcatcttga atctctttgg atcatggtta tttgatgcag
     1621 catttgttca ctgtaaactt cataatggga taaacagaga cagcagcatg actgccatta
     1681 caacacaagc tagcatggag tttcgacgga aagggtcaca aatgtccaca gacaccatgg
     1741 tttccaatcc tatgtttgat gcaagtgaat ttcctgataa ctatgaagca ggaagagctg
     1801 aggcttgtgg gacactgtgt aggatttttt gtagcaagaa gactggagaa gagattctgc
     1861 cagcttattt atccagattt tacatgcttt taattcaagg tttgcagata aatgattatg
     1921 tgtgccatcc tgtcttggcc agcgttattc taaactctcc tcctttgttc tgctgtgact
     1981 tgaaagggat tgatgttgtg gttccttact ttatttcagc tcttgaaacc attttgcctg
     2041 acagagaact ctcaaaattc aaaagctatg taaatccaac agaattgcga agatcctcca
     2101 ttaatatcct gctttctttg ttgcccctcc ctcatcattt tggcacagtc aaatctgagg
     2161 tggtcctgga aggaaagttt agtaacgatg acagctcttc ttatgataaa ccaataactt
     2221 ttctgtccct gaagttgaga cttgtgaata tattaatagg tgccttgcaa actgaaacgg
     2281 accccaacaa cacccaaatg atattagggg caatgttaaa tattgttcaa gattcagcac
     2341 ttttggaagc cattggttgc cagatggaga tgggtggtgg agaaaataac ctgaagagtc
     2401 atagtcgcac caatagtggt attagttcag caagtggtgg aagcacggag cccacgactc
     2461 ccgatagtga gagacctgct caagctctct taagagatta tgctcttaat acagattcag
     2521 ctgctgggct cctgattcgc agcattcatc tcgtcaccca aagactcaac tcccagtggc
     2581 gccaagacat gagcatatca ctggcagctc tagagctcct ctctggcctt gcaaaggtaa
     2641 aagtgatggt tgactcagga gaccggaagc gagccatcag ttctgtgtgc acctacattg
     2701 tttatcagtg tagtcggcca gctcctttac actccaggga tctgcactcc atgatagtgg
     2761 cagcttttca gtgtctctgt gtctggctga cagagcaccc tgatatgctt gatgaaaagg
     2821 actgccttaa ggaagtactg gagattgtgg aactgggtat ctcaggaagt aagtccaaga
     2881 acaatgagca agaggtcaag tacaaaggag ataaggagcc aaaccctgca tctatgaggg
     2941 taaaggatgc tgctgaagcc accctaacat gcattatgca gttgctcggc gcatttcctt
     3001 cacctagtgg tcctgcctct ccttgtagtc ttgtgaatga gaccactttg attaaatact
     3061 ccaggctgcc aaccataaac aagcatagtt tccggtactt tgtcttggat aacagtgtca
     3121 tcctggcaat gctggaacaa cctcttggaa atgagcagaa tgattttttc ccctctgtca
     3181 ctgtgctggt ccggggaatg tctggaagac ttgcttgggc acaacagctt tgtcttttac
     3241 ccagaggagc aaaagcaaat cagaagcttt ttgtacctga acctcgccca gttcctaaaa
     3301 atgacgttgg atttaaatat tctgtgaaac atcggccatt tcctgaagag gtggacaaga
     3361 ttccttttgt gaaagcagat ctcagcattc cagatttgca tgaaatagtc actgaagaat
     3421 tagaagagag acacgaaaaa ttaaggagtg gcatggccca gcagattgct tatgaaatac
     3481 accttgagca acagagtgag gaggaattgc agaagagaag ttttcctgac ccagttacgg
     3541 attgcaagcc cccgcctcct gcccaggaat tccaaacagc ccgccttttt ctctcacact
     3601 ttggattttt gtccttagaa gcactgaagg aacctgcaaa tagtcgtcta cctcctcacc
     3661 ttattgcact tgattccacg atacctggat tttttgatga cattgggtat ctggatctct
     3721 tgccatgtcg tccttttgac acagttttta ttttctatat gaagccaggt cagaaaacga
     3781 accaagagat tttaaagaat gtggagtctt ccagaactgt tcagccacat ttcctagaat
     3841 ttttgctttc ccttggctgg tcagtagatg tgggcagaca ccctggttgg actgggcatg
     3901 tttctaccag ttggtctatt aattgttgtg atgatggtga aggatctcaa caagaagtga
     3961 tttcctctga agatattgga gctagcattt tcaatggaca gaagaaggtg ctgtattatg
     4021 ctgatgccct tacagaaatt gcttttgtgg ttccttctcc tgtggagtcc ttaactgatt
     4081 cattggaaag taacatctcg gaccaagata gtgattcaaa tatggatctt atgccaggaa
     4141 ttctgaaaca gccatccctg acacttgagc ttttccccaa tcatacagac aatcttaatt
     4201 cctcacagag gctcagtccc agttccagaa tgaggaagct gcctcagggt cgccctgttc
     4261 ctccccttgg acctgagaca agagtttctg tagtctgggt ggaacgctat gatgatatag
     4321 aaaactttcc cctctcagag ctgatgacag agatcagtac tggtgtggaa actactgcaa
     4381 atagtagcac ttcactgaga tctacaactc ttgaaaaaga agttcctgtc atcttcatcc
     4441 accctttaaa cactggatta ttccggataa aaattcaagg agccactgga aaatttaata
     4501 tggtcatccc tcttgtggat gggatgattg tcagcaggcg agctcttggc tttctggtga
     4561 ggcagactgt aattaacatt tgtagaagaa agagactgga aagtgactcc tacagtcccc
     4621 cccatgtccg ccggaaacag aaaatcaccg acattgtcaa caagtaccgg aacaagcagc
     4681 tggagccaga gttttatact tcacttttcc aggaggttgg actcaagaac tgcagttctt
     4741 agaccactga atttctaaga ctgttgaact ccagtttggg aactataaca cagcagaaca
     4801 gtttgatagg tgatcactgt aaaaataaaa acaaatcact cccaagagct tactgtttaa
     4861 tcaccagaat agaagaaaca cattataacc catttgatag aagactttgg gctatctagt
     4921 gaaatgggct cccagacaca atcacactcc tgctgataat gatgatatac attttagcca
     4981 taaactttct tttaaaagtg acaattttag ttaaacataa gccttttgag gagaaaggct
     5041 tttatgcatc tcagttaaac acgtgcattg gtagtatcaa caaatttgca atatagaagt
     5101 tgaagatagt tttttacctc actttttagg aggttgtatt caaaattaaa atctcagaat
     5161 cttacaggac atttaaagac tcatgttgat agcatggagg agaaggaaag aagtcacagc
     5221 cttctactca gttgtaggtc ttcttgtcat ccagctgtca cactgacaaa aagaaaagat
     5281 gatacatgtt ttttgctcag ataagaagcc tgacattaaa agatgtcata tttttttctc
     5341 cacatttcaa aaagttgtcc ttctcatcac tgcacagatc tgtctgaaag cctcagtttc
     5401 tgagtgaccc aggaacagat cagaaatgga gcatggcctt gtcctttaat ggggatgcaa
     5461 ataaagtttg tggggttaaa agttataaga cagcagtgat accccactct ctccattatt
     5521 gtccagcggg gtgacataat gacaggttaa atatttgtga ttcattgatt aaatattatt
     5581 taaagaaatg taaattcaca ataagggttg aaaattattt ggtttcatcc attgtctctt
     5641 atttcaggac caagcagcaa actgcagtag tttgtgaagg attctaatat ggggttcagg
     5701 aatagcctct caacgctact aattcagatc tctcccagag aactactgga tttcctcata
     5761 attgacaaac atgagtgacc acctctttgg gtggctactg ttagaaatgg ctgttgtcat
     5821 gttttctgga ctttgccagc caacagatcc ctgccaggtt ttggaaatac ttctattacc
     5881 tcgctgctac ttttctgcag ggataaaact tttgaggtgg ccagacccag aacatccaag
     5941 gattcctgtt acagtgctac agtatacact gctcatttat cctattctca tgtgctttct
     6001 tctttagtaa gattatttta agaaaataag tgatatttaa agtccaaaga ggaatgatca
     6061 cagttgtata aggggtgttt tcccacttga actctgatgt cagtcgactg tgggtcagag
     6121 ctacaaccat ctgtttggtt tgatgttttg gtggtttact tacggagtgg ggatagtgtg
     6181 agacctaatt ccctgtgcaa atgtctctta ttccagaaat gtgcattttg tcatctataa
     6241 gcaagaaata tgggcatagc agctcttggt ttaaagtttg ccataacctg ttcatgtttg
     6301 ttttaagctc aggtaaagat aacctcctct ttctatgact ccagtttcca ttcaggttat
     6361 agtattattc aatagttgat tttcttttta agctgggcaa taaattgatg tttccagatg
     6421 gtaacatggg agagggcata taggataaag atgagcaaat tctaccctaa aaatgttcta
     6481 gtagttcaca ggaagaagat gaggtttaat aactttcaag gtaattctag attgacattt
     6541 tgaggggaaa atgggctctt gttctagttg aagtgagcag agaaggctat aaattaatat
     6601 gtaacttaca gcattccaga ggttaaaaat aactgatgca gatgtacttc ttcagtgtga
     6661 ttcttcagat caaactttta cttttggcat agttaatttc agaaaaatgt gctgtatgtg
     6721 tgtgtgtatg agggttggtc ttgctgatcc ttcagttagc tctaaattct ggcaactcct
     6781 tgtaattcca atgtatttga tacatgaaca atcatgttga atgcatttgt gatctgggag
     6841 acttcctcgt cttccaggga aggaaggatg tgcagcccct gaaggcatga aactcccagt
     6901 gtgtacggag ccagtggaat atgggatacc cataccttac caggcgctgg ttccttctgc
     6961 tcacaataac atctgcccaa agagggagtg ggaagaacgc ttagctcttt cactagtatg
     7021 gatttgagtt catggtcact atttttaccc acctgccttt gttaaaaatc actttgagta
     7081 gaatagcact ggaggaacat atttagcacc taatattaat atttagtagt ccattgataa
     7141 atttgccagc atatgttcta gcctctgggg ggaaaccagg accacttttg tctgtggctt
     7201 aaacagttca gttgctatat ctgttgggta tgccgggggt ggatgagtgt ggcattccgt
     7261 gaagaggaag gtggtaagta aggtttccct tctactgcct tcttaagttg caggagggag
     7321 cttttctcct cccctctggt tgggagcact gaggacagtg aggagggctt ttaccttgtt
     7381 aatcctttcc ttatttagct agctttcctt tttgtctagg gcttcctctt gagaccctct
     7441 tccatccatt gggcctttga aaggactaat cagacacaca cacacacaca cacacacaca
     7501 cacacactcg catactcatg cacattttcc ttcatttcca gatcctttat ttcagagcag
     7561 cccattttcc tctggattca ttgatgaata caagtaccca cacctttggc cagtaatgtc
     7621 agttacctgc tgcaggttct gtgtatgagg ccttcatgaa cggttacctt ctccatacac
     7681 tagggaagca tttgtcagac tctgcagact gggttctaga gaggcagagt ctttaagagt
     7741 attcatttct tctggaaggt ggagctttac ccaaagtgga agttagcctt gctcaaagat
     7801 gtgttttgtg gtaggtggta aaaataaata aataaataaa taataaaaaa agaaacatgt
     7861 attggaggta atttgacact gctgctggca gtagttctct attcaccatt ttaaagccca
     7921 ttcaggttct ctcttcctga aaagaactga ttgctgtgtt tacatgaaat gacattggag
     7981 tcagatggtc tgttttaaag atttccatga cagcctcttt tcctgagttg gagagattgg
     8041 aggtggtcta tccgtacgat gtggaatcaa acggtgggtt tcttagtagc taaagaagcc
     8101 atgtacttct agtgtgtttc tcagaatatc aactcatgtt cttcagatgc ttttcttttt
     8161 ttaatggtga gggaaaaggt ataatttggg attccacagt gccttgcata tagtaggcgc
     8221 ccagtaaata cttgttgaag caaaccaagt ttcccaagtc ctcatctctt atagtgacca
     8281 agacatcttt ctcctctgaa gggcttggca gttgtggcta aaaaataagc agtatcatta
     8341 tttgcttgaa atcatatata cagtttgtat gaatttcagt atgttgccaa gacatgattt
     8401 tttcttattg tattttctgt aaatatttct ggcactgaac tgtaaagtaa aggcaaagtg
     8461 taaatatgaa ggcgtgcccg tgccccttgc ctcctgtgtt tcatcttcgt cggttaggga
     8521 agaaggtcca gaggtttgtt tgtatttatg ccgatccttt gtccagaaga agcccatgga
     8581 atattgaatg taatacattt agtcaattaa attttaagga gattcttatc taa
//
