LOCUS       NM_020343               9545 bp    mRNA    linear   PRI 01-JUN-2024
DEFINITION  Homo sapiens Ral GTPase activating protein catalytic subunit alpha
            2 (RALGAPA2), mRNA.
ACCESSION   NM_020343 XM_046600 XM_933547 XM_933550 XM_941711 XM_945389
            XM_945392
VERSION     NM_020343.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9545)
  AUTHORS   Fattahi Dolatabadi,N., Dehghani,A., Shahand,E., Yazdanshenas,M.,
            Tabatabaeian,H., Zamani,A., Azadeh,M. and Ghaedi,K.
  TITLE     The interaction between MALAT1 target, miR-143-3p, and RALGAPA2 is
            affected by functional SNP rs3827693 in breast cancer
  JOURNAL   Hum Cell 33 (4), 1229-1239 (2020)
   PUBMED   32880825
  REMARK    GeneRIF: The interaction between MALAT1 target, miR-143-3p, and
            RALGAPA2 is affected by functional SNP rs3827693 in breast cancer.
REFERENCE   2  (bases 1 to 9545)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 9545)
  AUTHORS   Uegaki,M., Kita,Y., Shirakawa,R., Teramoto,Y., Kamiyama,Y.,
            Saito,R., Yoshikawa,T., Sakamoto,H., Goto,T., Akamatsu,S.,
            Yamasaki,T., Inoue,T., Suzuki,A., Horiuchi,H., Ogawa,O. and
            Kobayashi,T.
  TITLE     Downregulation of RalGTPase-activating protein promotes invasion of
            prostatic epithelial cells and progression from intraepithelial
            neoplasia to cancer during prostate carcinogenesis
  JOURNAL   Carcinogenesis 40 (12), 1535-1544 (2019)
   PUBMED   31058283
  REMARK    GeneRIF: loss of RalGAP function has a distinct role in promoting
            progression from prostatic intraepithelial neoplasia to invasive
            adenocarcinoma.
REFERENCE   4  (bases 1 to 9545)
  AUTHORS   Gao,P., Liu,S., Yoshida,R., Shi,C.Y., Yoshimachi,S., Sakata,N.,
            Goto,K., Kimura,T., Shirakawa,R., Nakayama,H., Sakata,J.,
            Kawashiri,S., Kato,K., Wang,X.Y. and Horiuchi,H.
  TITLE     Ral GTPase Activation by Downregulation of RalGAP Enhances Oral
            Squamous Cell Carcinoma Progression
  JOURNAL   J Dent Res 98 (9), 1011-1019 (2019)
   PUBMED   31329042
  REMARK    GeneRIF: Low RalGAPalpha2 expression was associated with a reduced
            survival rate in patients with oral squamous cell carcinoma
REFERENCE   5  (bases 1 to 9545)
  AUTHORS   Cox,A.J., Hugenschmidt,C.E., Raffield,L.M., Langefeld,C.D.,
            Freedman,B.I., Williamson,J.D., Hsu,F.C. and Bowden,D.W.
  TITLE     Heritability and genetic association analysis of cognition in the
            Diabetes Heart Study
  JOURNAL   Neurobiol Aging 35 (8), 1958 (2014)
   PUBMED   24684796
REFERENCE   6  (bases 1 to 9545)
  AUTHORS   Saito,R., Shirakawa,R., Nishiyama,H., Kobayashi,T., Kawato,M.,
            Kanno,T., Nishizawa,K., Matsui,Y., Ohbayashi,T., Horiguchi,M.,
            Nakamura,T., Ikeda,T., Yamane,K., Nakayama,E., Nakamura,E.,
            Toda,Y., Kimura,T., Kita,T., Ogawa,O. and Horiuchi,H.
  TITLE     Downregulation of Ral GTPase-activating protein promotes tumor
            invasion and metastasis of bladder cancer
  JOURNAL   Oncogene 32 (7), 894-902 (2013)
   PUBMED   22450745
  REMARK    GeneRIF: Downregulation of Ral GTPase-activating protein promotes
            tumor invasion and metastasis of bladder cancer.
REFERENCE   7  (bases 1 to 9545)
  AUTHORS   Lauc,G., Huffman,J.E., Pucic,M., Zgaga,L., Adamczyk,B., Muzinic,A.,
            Novokmet,M., Polasek,O., Gornik,O., Kristic,J., Keser,T.,
            Vitart,V., Scheijen,B., Uh,H.W., Molokhia,M., Patrick,A.L.,
            McKeigue,P., Kolcic,I., Lukic,I.K., Swann,O., van Leeuwen,F.N.,
            Ruhaak,L.R., Houwing-Duistermaat,J.J., Slagboom,P.E., Beekman,M.,
            de Craen,A.J., Deelder,A.M., Zeng,Q., Wang,W., Hastie,N.D.,
            Gyllensten,U., Wilson,J.F., Wuhrer,M., Wright,A.F., Rudd,P.M.,
            Hayward,C., Aulchenko,Y., Campbell,H. and Rudan,I.
  TITLE     Loci associated with N-glycosylation of human immunoglobulin G show
            pleiotropy with autoimmune diseases and haematological cancers
  JOURNAL   PLoS Genet 9 (1), e1003225 (2013)
   PUBMED   23382691
REFERENCE   8  (bases 1 to 9545)
  AUTHORS   Bohm,D., Keller,K., Pieter,J., Boehm,N., Wolters,D., Siggelkow,W.,
            Lebrecht,A., Schmidt,M., Kolbl,H., Pfeiffer,N. and Grus,F.H.
  TITLE     Comparison of tear protein levels in breast cancer patients and
            healthy controls using a de novo proteomic approach
  JOURNAL   Oncol Rep 28 (2), 429-438 (2012)
   PUBMED   22664934
REFERENCE   9  (bases 1 to 9545)
  AUTHORS   Shirakawa,R., Fukai,S., Kawato,M., Higashi,T., Kondo,H., Ikeda,T.,
            Nakayama,E., Okawa,K., Nureki,O., Kimura,T., Kita,T. and
            Horiuchi,H.
  TITLE     Tuberous sclerosis tumor suppressor complex-like complexes act as
            GTPase-activating proteins for Ral GTPases
  JOURNAL   J Biol Chem 284 (32), 21580-21588 (2009)
   PUBMED   19520869
REFERENCE   10 (bases 1 to 9545)
  AUTHORS   Gridley,S., Chavez,J.A., Lane,W.S. and Lienhard,G.E.
  TITLE     Adipocytes contain a novel complex similar to the tuberous
            sclerosis complex
  JOURNAL   Cell Signal 18 (10), 1626-1632 (2006)
   PUBMED   16490346
  REMARK    GeneRIF: The cDNA sequence for human AS250 is reported, and the
            sites that undergo phosphorylation upon insulin treatment of
            adipocytes have been identified by tandem mass spectrometry.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL157718.10, DQ310704.1, BC171842.1, AL161658.21 and AY007097.1.
            
            On Nov 23, 2018 this sequence version replaced NM_020343.3.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000202677.12/ ENSP00000202677.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-164               AL157718.10        7268-7431           c
            165-2704            DQ310704.1         1-2540
            2705-5482           BC171842.1         2485-5262
            5483-5781           DQ310704.1         5319-5617
            5782-5844           BC171842.1         5562-5624
            5845-9185           AL161658.21        56447-59787         c
            9186-9447           AY007097.1         1672-1933
            9448-9545           AL161658.21        56087-56184         c
FEATURES             Location/Qualifiers
     source          1..9545
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p11.23"
     gene            1..9545
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /note="Ral GTPase activating protein catalytic subunit
                     alpha 2"
                     /db_xref="GeneID:57186"
                     /db_xref="HGNC:HGNC:16207"
                     /db_xref="MIM:618836"
     exon            1..270
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     CDS             165..5786
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /note="RapGAPalpha2; ral GTPase-activating protein alpha
                     subunit 2; akt substrate AS250; 250 kDa substrate of Akt;
                     Ral GTPase activating protein, alpha subunit 2
                     (catalytic); Ral GTPase activating protein catalytic alpha
                     subunit 2"
                     /codon_start=1
                     /product="ral GTPase-activating protein subunit alpha-2"
                     /protein_id="NP_065076.2"
                     /db_xref="CCDS:CCDS46584.1"
                     /db_xref="GeneID:57186"
                     /db_xref="HGNC:HGNC:16207"
                     /db_xref="MIM:618836"
                     /translation="MFSRRSHGDVKKSTQKVLDPKKDVLTRLKHLRALLDNVDANDLK
                     QFFETNYSQIYFIFYENFIALENSLKLKGNNKSQREELDSILFLFEKILQFLPERIFF
                     RWHYQSIGSTLKKLLHTGNSIKIRCEGIRLFLLWLQALQTNCAEEQVLIFACLVPGFP
                     AVMSSRGPCTLETLINPSPSVADVKIYPEEITPLLPAISGEKIAEDQTCFFLQILLKY
                     MVIQAASLEWKNKENQDTGFKFLFTLFRKYYLPHLFPSFTKLTNIYKPVLDIPHLRPK
                     PVYITTTRDNENIYSTKIPYMAARVVFIKWIVTFFLEKKYLTATQNTKNGVDVLPKII
                     QTVGGGAVQERAPELDGGGPTEQDKSHSNSSTLSDRRLSNSSLCSIEEEHRMVYEMVQ
                     RILLSTRGYVNFVNEVFHQAFLLPSCEIAVTRKVVQVYRKWILQDKPVFMEEPDRKDV
                     AQEDAEKLGFSETDSKEASSESSGHKRSSSWGRTYSFTSAMSRGCVTEEENTNVKAGV
                     QALLQVFLTNSANIFLLEPCAEVPVLLKEQVDACKAVLIIFRRMIMELTMNKKTWEQM
                     LQILLRITEAVMQKPKDKQIKDLFAQSLAGLLFRTLMVAWIRANLCVYISRELWDDFL
                     GVLSSLTEWEELINEWANIMDSLTAVLARTVYGVEMTNLPLDKLSEQKEKKQRGKGCV
                     LDPQKGTTVGRSFSLSWRSHPDVTEPMRFRSATTSGAPGVEKARNIVRQKATEVEECQ
                     QSENAPAAGSGHLTVGQQQQVLRSSSTSDIPEPLCSDSSQGQKAENTQNSSSSEPQPI
                     QENKGHVKREHEGITILVRRSSSPAELDLKDDLQQTQGKCRERQKSESTNSDTTLGCT
                     NEAELSMGPWQTCEEDPELNTPTDVVADADARHWLQLSPTDASNLTDSSECLTDDCSI
                     IAGGSLTGWHPDSAAVLWRRVLGILGDVNNIQSPKIHARVFCYLYELWYKLAKIRDNL
                     AISLDNQSSPSPPVLIPPLRMFASWLFKAATLPNEYKEGKLQAYRLICAMMTRRQDVL
                     PNSDFLVHFYLVMHLGLTSEDQDILNTIIRHCPPRFFSLGFPGFSMLVGDFITAAARV
                     LSTDILTAPRSEAVTVLGSLVCFPNTYQEIPLLQSVPEVNEAITGTEDVKHYLINILL
                     KNATEEPNEYARCIAVCSLGVWICEELAQCTSHPQVKEAINVIGVTLKFPNKIVAQVA
                     CDVLQLLVSYWEKLQMFETSLPRKMAEILVATVAFLLPSAEYSSVETDKKFIVSLLLC
                     LLDWCMALPVSVLLHPVSTAVLEEQHSARAPLLDYIYRVLHCCVCGSSTYTQQSHYIL
                     TLADLSSTDYDPFLPLANVKSSEPVQYHSSAELGNLLTVEEEKKRRSLELIPLTARMV
                     MAHLVNHLGHYPLSGGPAILHSLVSENHDNAHVEGSELSFEVFRSPNLQLFVFNDSTL
                     ISYLQTPTEGPVGGSPVGSLSDVRVIVRDISGKYSWDGKVLYGPLEGCLAPNGRNPSF
                     LISSWHRDTFGPQKDSSQVEEGDDVLDKLLENIGHTSPECLLPSQLNLNEPSLTPCGM
                     NYDQEKEIIEVILRQNAQEDEYIQSHNFDSAMKVTSQGQPSPVEPRGPFYFCRLLLDD
                     LGMNSWDRRKNFHLLKKNSKLLRELKNLDSRQCRETHKIAVFYIAEGQEDKCSILSNE
                     RGSQAYEDFVAGLGWEVDLSTHCGFMGGLQRNGSTGQTAPYYATSTVEVIFHVSTRMP
                     SDSDDSLTKKLRHLGNDEVHIVWSEHSRDYRRGIIPTAFGDVSIIIYPMKNHMFFIAI
                     TKKPEVPFFGPLFDGAIVSGKLLPSLVCATCINASRAVKCLIPLYQSFYEERALYLEA
                     IIQNHREVMTFEDFAAQVFSPSPSYSLSGTD"
     exon            271..381
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            382..434
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            435..492
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            493..536
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            537..714
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            715..830
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            831..969
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            970..1169
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            1170..1397
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            1398..1565
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            1566..1703
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1852
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            1853..1964
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            1965..2202
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            2203..2367
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            2368..2505
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            2506..2603
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            2604..2694
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            2695..2871
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            2872..3065
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            3066..3164
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            3165..3320
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            3321..3449
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            3450..3578
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            3579..3637
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            3638..3746
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            3747..3857
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            3858..3926
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            3927..4064
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            4065..4248
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            4249..5020
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            5021..5092
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            5093..5216
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            5217..5372
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            5373..5531
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            5532..5659
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            5660..5781
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            5782..5821
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     exon            5822..9545
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9175..9180
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /note="hexamer: GATAAA"
     polyA_site      9197
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /note="major polyA site"
     regulatory      9428..9433
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /note="hexamer: AATAAA"
     polyA_site      9447
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /note="major polyA site"
     regulatory      9477..9482
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /note="hexamer: ATTATA"
     polyA_site      9495
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
     regulatory      9516..9521
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
                     /note="hexamer: AATAAA"
     polyA_site      9545
                     /gene="RALGAPA2"
                     /gene_synonym="AS250; bA287B20.1; C20orf74; dJ1049G11;
                     dJ1049G11.4; p220"
ORIGIN      
        1 ggagacacag ctgaggcggc ggcggcggcg gcggcggcgg cagcagcggg gctgggggcg
       61 gcgagtggcc cgcgcgacac gcgccggcct cgaccctatg ctttgattcg cgtggcgcag
      121 gcgcctactg ccccgccggc ggggcccggg cttcctgccg cgggatgttc tcccgaagga
      181 gccacgggga tgtgaagaag tccacccaga aggtgctgga cccgaagaag gacgtgctga
      241 cccgcctgaa gcacctgcgg gcgctgctgg ataatgtgga tgcaaatgat cttaagcagt
      301 tttttgagac caactattct cagatatatt tcatcttcta tgaaaatttt atagcactgg
      361 aaaatagttt gaaattaaaa gggaataata agtcacaaag ggaggagctg gactccatcc
      421 tcttcctttt tgaaaaaata ctgcagtttc tacctgaacg aattttcttt cgatggcatt
      481 accagagtat aggctcaact ttaaagaagc ttctacacac tggaaattct attaagataa
      541 gatgtgaagg aatcaggctg tttcttctgt ggcttcaagc acttcagaca aactgtgcag
      601 aagagcaggt tctgattttt gcttgcctgg tgcctggttt cccagcagtc atgtcatcca
      661 ggggcccttg cacactggag acactcatca atcccagccc tagtgtagct gatgtaaaga
      721 tatatccaga agaaatcact ccactcctac cagccatatc aggggagaag attgctgagg
      781 accaaacctg cttttttctt caaatactgt tgaagtatat ggttattcag gctgcgagct
      841 tggagtggaa gaataaggag aatcaagata ctggttttaa atttcttttt acattgtttc
      901 gaaagtatta tcttcctcat ttatttccat catttactaa gttaacaaac atctacaaac
      961 ctgtacttga catcccccat ttgagaccaa agcctgtgta cattactacc actcgagaca
     1021 atgagaacat ttacagtaca aagattccat atatggcagc tcgtgttgtt tttattaagt
     1081 ggattgtaac cttctttttg gaaaaaaagt atctaactgc aacacaaaac actaaaaatg
     1141 gagttgatgt attgcctaaa atcatccaga ctgttggtgg tggtgctgtg caggagagag
     1201 cgcctgagct ggatggtggt gggcccacgg agcaggacaa aagccattct aacagcagca
     1261 ccttgtcgga ccgaagactc agcaactcca gcctctgtag cattgaagaa gagcaccgaa
     1321 tggtgtatga aatggtacag cggattctct tgtcaacacg aggttatgtc aacttcgtga
     1381 atgaagtatt tcaccaggca tttttgttgc cttcctgtga gatagctgta acaagaaaag
     1441 tagttcaagt gtacagaaag tggattctcc aggacaaacc tgtgttcatg gaggagccag
     1501 atagaaaaga tgttgcccaa gaagatgctg aaaaattagg attttccgag actgatagca
     1561 aggaggcctc atctgaaagt tctggtcata aacgatcttc cagttgggga cgcacatact
     1621 ccttcacaag tgcaatgagc agagggtgtg tgacagagga ggaaaataca aatgtgaaag
     1681 ccggcgtcca ggctttgttg caggtatttt tgacgaactc tgcaaacatc tttttgttgg
     1741 aaccatgtgc tgaagttcct gtgctcttga aagaacaagt tgatgcttgt aaagctgttt
     1801 tgattatttt taggcgcatg ataatggagc ttacaatgaa taaaaagaca tgggaacaga
     1861 tgttgcaaat actactcagg ataacagaag ctgtcatgca gaagccaaag gataaacaaa
     1921 taaaggactt gtttgcccag agcttggcag ggttactatt taggacgctc atggtagctt
     1981 ggatccgagc aaacctctgt gtgtacattt ctcgagagct ctgggatgac tttcttggtg
     2041 tgctgtcctc cctcacggaa tgggaggaac ttataaacga gtgggccaac attatggact
     2101 ccttgacagc agtgcttgca agaaccgttt atggagttga gatgacaaac ctacctctgg
     2161 ataaattaag tgaacaaaag gaaaagaagc aacgaggcaa aggctgtgtt ctagatcctc
     2221 agaaaggaac caccgtgggg aggtcctttt ctctcagctg gcggagccac ccagatgtga
     2281 ccgaaccgat gcgatttagg agtgccacca cgtctggagc accgggagtg gaaaaggcaa
     2341 gaaatattgt tcgccaaaaa gcaactgaag tggaggagtg tcaacagtca gaaaatgcac
     2401 ctgcagccgg atctggccat ctcacagtgg gacagcagca gcaggtcctt cggagcagca
     2461 gcacctccga catccccgag ccgctgtgct cagattcttc tcagggtcaa aaggcagaaa
     2521 acacacagaa ttcgagttct tcagagcctc agcctattca agagaataaa ggacatgtga
     2581 agagagaaca tgaaggaata acaatcttag ttcgaagaag cagcagccct gctgaattgg
     2641 atttgaaaga tgatttgcag cagacacaag gaaaatgtag ggaaagacag aagagtgaaa
     2701 gtaccaacag tgacacaact ctgggctgta ccaatgaggc agagctgtcc atgggcccat
     2761 ggcagacctg tgaggaagac ccagaactga atactcccac agatgttgtg gctgatgctg
     2821 atgcccgtca ttggttacaa ctgagtccca ccgatgcttc aaatttaaca gatagcagcg
     2881 agtgcctcac agatgactgt agtataatcg ccggggggag cctcactggt tggcacccag
     2941 actctgctgc tgtgttatgg cgaagggtct tggggatcct cggagatgtg aataacatcc
     3001 agtcacccaa gatccatgcc agagttttct gctatctcta tgaactctgg tacaaactag
     3061 caaagatacg ggataatcta gcaataagcc tggataacca gtcttctcca tctcctccag
     3121 ttttgatccc accactcaga atgtttgcat catggctgtt taaggcggcg acactgccaa
     3181 atgaatataa ggaaggcaaa ctacaagcct acaggctgat ctgtgccatg atgaccagac
     3241 gccaggacgt tctgccaaac tcagatttcc tggtgcattt ttaccttgtg atgcacctgg
     3301 gattaaccag cgaggatcag gatatcttaa atacgatcat aaggcactgt ccaccccgct
     3361 ttttctccct gggttttcct ggcttctcaa tgctggtggg ggacttcatc acggccgctg
     3421 ccagggtcct tagcacagac attttgacgg cgcctcgttc agaggctgtc actgtcctcg
     3481 gctctctggt ctgctttcca aatacctacc aggagattcc tttactgcag tcagtgccag
     3541 aagtaaatga ggccattaca ggaactgaag atgtcaagca ttacctcata aatattttac
     3601 tgaagaatgc cacagaagaa ccaaatgaat atgcaagatg cattgctgtt tgctcccttg
     3661 gggtctggat atgtgaagag cttgcacagt gtacaagcca ccctcaggtg aaagaggcca
     3721 tcaatgtgat aggagtaact ctgaagtttc ccaacaaaat cgtggcccag gtagcttgcg
     3781 atgtccttca gttgctggtt tcctactggg agaagcttca gatgtttgaa acctctctgc
     3841 ctcggaaaat ggcagaaatc ctcgtggcca cagttgcttt tcttttacca agtgcagagt
     3901 actcctcagt ggaaacagac aagaagttta ttgtgtcctt gctactctgc ctcttggact
     3961 ggtgcatggc attgcccgtg agtgtccttc tccaccccgt gtccacagca gtcctagagg
     4021 agcagcattc ggccagagcc cccttgctgg attatatcta cagggttttg cactgctgtg
     4081 tgtgtggctc aagcacgtac acccaacaga gtcactacat actgaccctg gctgacttgt
     4141 catccacgga ttatgacccc ttcctgccac tggcaaatgt gaagagctct gagccagtcc
     4201 agtatcattc atcagcagaa ttgggtaacc tgctgactgt tgaagaggag aagaagagaa
     4261 gaagtttgga gttgatccct ttgactgctc gaatggtgat ggcccacctg gtgaaccacc
     4321 tggggcacta ccccctcagt gggggccctg ccatactgca cagccttgtc agcgagaacc
     4381 atgacaatgc ccatgtggaa ggctccgagc tgtcctttga ggtgttcaga agtccaaacc
     4441 tgcagctgtt tgtatttaat gatagcaccc tcatctccta ccttcagaca cccacagaag
     4501 gaccggtagg gggatcacca gtgggctctc tctctgatgt gagagtaatt gtgagggata
     4561 tctcagggaa gtactcttgg gatggtaagg ttttatatgg acctttggaa ggctgcttag
     4621 cacccaatgg aagaaatcct tcatttctga tttcgagctg gcatcgtgac acatttggac
     4681 ctcagaaaga ctcttctcaa gttgaggagg gggatgatgt tcttgacaaa ttacttgaaa
     4741 acattggcca tacaagtcct gaatgccttt taccgtcaca gctaaatcta aatgaacctt
     4801 ccctaacccc atgtggcatg aactatgacc aagagaagga aatcattgag gtcattttgc
     4861 gccaaaatgc tcaagaggat gagtatatcc agagtcataa cttcgattct gcaatgaaag
     4921 tcaccagcca agggcagccc tccccagtgg agccccgagg acccttttat ttctgcaggt
     4981 tattgcttga tgacttggga atgaattctt gggacagaag gaagaatttt catctattga
     5041 agaaaaattc aaaattattg agagagctga aaaatttgga ctcccgccag tgccgtgaga
     5101 cacacaaaat cgcagtgttt tacattgctg aaggtcaaga agacaagtgt tcaatcctct
     5161 ctaatgaaag aggaagccaa gcatatgaag actttgttgc tggacttgga tgggaggtgg
     5221 atctctccac ccactgtggg ttcatgggtg gccttcagcg caatggcagc accgggcaga
     5281 cggcccctta ctatgctacc tcaactgtgg aagtgatttt ccatgtttcc actcgaatgc
     5341 cgtcagactc agatgattcc ctcaccaaaa agcttcgtca cttggggaat gacgaggtcc
     5401 atatcgtctg gtctgaacac tccagagact accgcagggg tattatccca actgcctttg
     5461 gagatgtttc aatcattatt tacccaatga agaatcacat gttcttcatc gcgataacga
     5521 agaaacctga ggttccattt tttgggcctc tgtttgatgg agccatagtg agtgggaagc
     5581 tgctgccaag ccttgtatgt gccacgtgca tcaacgccag cagggctgtg aagtgcctca
     5641 tcccactcta ccagagcttc tatgaagagc gagctctgta tctcgaagca ataattcaga
     5701 accaccgcga agtaatgaca ttcgaggatt tcgcagccca agtcttttct ccctctccca
     5761 gctactccct cagcggaacg gattaaaaca cgtaaaggtc tcatttcaat atttgagcaa
     5821 ggggccctgg cctccagtct gagtgctgac ctcgaagagc ccctgagtga ggaggagaca
     5881 gaacaccctc tcctgcctct gccccgagcc actaaaccca gaatctccag gaaatttcgt
     5941 tgctgtacta gtgccctcag caggtcttcc cattagtgga cttctgaagc ccatggtgaa
     6001 tcccactacc gtgtctcaga tgggactcaa actaggctgt atcatcttgt cctgaaacct
     6061 cacaaacctt ggacttccaa agaagaaaag aaaaagtcac caaaaaccag agatgaaccc
     6121 actgacacgt gggcactggt gtggctgctc tgggcagatg acagcaggaa aagattctgc
     6181 gctttcaggg agggggcaga gggcagcttg cttctgcacc ccaagttgtt tcttctcatt
     6241 tatgaaccca agcatgattc acaaaggggc tggcaaaaca attgtctaat tgaggaatct
     6301 gatctgcatt gattttgcta gttcctttta tgttaggatt tatggtttac tcaaaaattt
     6361 catcatcatg gaaattggct ttccccgggg tcttttgccc aggagaagtt ttacctcagt
     6421 tgtccagtgt tttcccacat ggtacctgac gcccgctaac taagcagagg ctctcctcac
     6481 cacgcagagg cgcttcaggt catgacagtg atgcccctat cttagaacac tcagaagatt
     6541 ccagaaatac agggtcctgt ccttgcataa gttcctgcag gacaggaagt gtcccaaggc
     6601 tcaaaacaac acagaccaca tccacacaaa aggcgcatgc cggcttcagg cgctaggctg
     6661 agacgggtac cccccgaatg gccttgtgcc tccaaaagcc tcacgctgca actttgctgc
     6721 tcctgtctgt tgcccagaga ggcctcacac aggcctcaga aatgtttctt gtcacacaaa
     6781 gcttttggta gccgattcca agcgagggat ttaatcctta catttttgcc catttggctc
     6841 caaggaaggc ttattagacc tgtttttttg ttgttgttgt ttttttttct taaaaacaaa
     6901 atgaatgaat gaatgaaaag agaagcaatg gtgagccctc tcctgctggg gcgccctggg
     6961 tgggccttgc ccgtcctgca gcagagaaat cagtcagcag cgaagagctg ctggcgcagg
     7021 ccccaggctg tgtcctggac ccttgacctg cctcagagct agaggtttgc tccaccccgc
     7081 acttcacttg ggaagtaaat ccaagaagct taccagagtc catcgccact gcgcaggctt
     7141 tgttctcaca gcggccaggt acagagcaac gccctgcagt gggaagggac agcagcacga
     7201 gagacgcagc ccctaactca gggcttatga tgggtgggct ggtcctggga aaggcgggcc
     7261 ctgtgcggga ggcctcccca ccttcctgtg ccctctgccc tctgcccttg cacacacttg
     7321 ttaccttcag gctgcttggc cacccaggcc tgaagaagcc ttctgaggct ctcaccccat
     7381 cagaacggcc attttcactg aggagccagt tttttccatt tgccatggag ctggtttggc
     7441 tggaacctgg cctttgtagg gaaaacgggg agactcagga gccagactca ctcttgggac
     7501 aaatgagaaa gagctgagcc agtcgttcct gtcggaaggg cgggcctgct catcctggcg
     7561 agaatttgct tttctgcttt ataggggatt gcaggccagg cccactgaca ggagcacctt
     7621 gagaaccacc tgaacagaag agaatgaggg tgccccaaag tggaaggaag cttctggccc
     7681 ctgggccgtt ttgggaaggg cccaggatac tcacgggggc tgaagggcaa tgtgaagagt
     7741 gactgcaagt cctggcattt tctgtggtgt cagcaaatgc ccctttattt taaatgattc
     7801 cagacatctg ggcagcatag cttggccacc atcactcagg cttgatcctg ctgttgagct
     7861 gctcctgtct cctgtttacc tcactcctgt tgggaaggcc atcgcatgct cctaggctcc
     7921 agagtcccct gtaggtctct accagcaggt cgcctctggg agaacgtttc atcttcaggc
     7981 tctagaaact cctgttgcgg aattgctggc tttagtagaa aagatatgtc aggatttaga
     8041 aatcgtattg aactctcctg gacttctgct ccatgatttc attttgcttt cacgaaccag
     8101 cgatccctac cttggggtgg gaaattcaat tgtaggcagc aaataactca accctcctgt
     8161 aacccctgag gattataacg accccatgac ccccgatgac agccagcagt ttggtgacta
     8221 gagttttttg agcctgccat tctcagtagc agctttggtc tggagatgga acgtggagca
     8281 gggaatctga cagccacgtt tctcgtgatc acgtggactg gagttgtttt tgttatctgg
     8341 ggtggaaaat tgcaatgatc aagtttcttt tttatttttt ttttacttag aaaagcaaat
     8401 attgtgtttt tgtaataaat ccctttcagc aaaattgcgg aggatcattc atccactagg
     8461 gccccacctg aggcacagcg ctgactcatc acggtggtac cagtaggtca gccatctgga
     8521 aacagaagtg gccctaattg ggccaaaatg tggtaagact cgctggcctc aggcagggcc
     8581 aggccagttc cctcacttct ctgcacacct attcccagat tccatccaga gcaaagctga
     8641 tgtttatcgt ctcattgtac ttaggctttc gtactttaaa aaattatgac tttttaaaaa
     8701 taagccttca gcagacagaa gtgaagaaat ttagcctggg ttgcctcagc aacaaagtct
     8761 gcggttccta agagccacat gttggggaag tggggtgacc caaacttggt gacataggtt
     8821 gattgatcag accctcagcc ttacaagcaa gagctcatgc agatccacag agccatgcac
     8881 atcctgtctt cctgaccttc ctcaggtggc accacctgcg ccccatggac ctgctgtatc
     8941 ggtgtcatgt ggaggtctga tgaatccttt aaaacctaac tgtgatgtcg taaatgtaac
     9001 tgtgctacct ttatatctac tgaagggcag cgtctgcatc cgagggaaat tcaacatgaa
     9061 acaaccatcg ttttagttgc actcagtctg tccatatgac atacacatga agtgaatgtt
     9121 tccttatgtt ttcaggtcta caatgtttag tacatgtaaa taaggaaaaa atttgataaa
     9181 tatggtaaat taagttatag cgtgtcattt gattcttatt ttgctgtctc ttgttattta
     9241 attttcctaa tgctccacat ctaggttcct tcctaggtgg tgggaacacc ttgcagacgt
     9301 gtgtgcacca ggacacaccg cggcccaggc agccacaccg agaaagcaca catctccact
     9361 gatggcagcc acccgactct ccagttaacc tcttttactc tttaattctg atttactgtg
     9421 atgatttaat aaagtggaaa cacagcactt cagtgctata tgtttctctc attctcatta
     9481 tagtttataa gaaaataaag ttcaccctgg catctaataa aaaggtggct ttcattttta
     9541 ttgga
//
