LOCUS       NM_001346249            9447 bp    mRNA    linear   PRI 06-APR-2024
DEFINITION  Homo sapiens Ral GTPase activating protein catalytic subunit alpha
            1 (RALGAPA1), transcript variant 11, mRNA.
ACCESSION   NM_001346249 XM_006720102
VERSION     NM_001346249.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9447)
  AUTHORS   Powell,C.J., Jenkins,M.L., Hill,T.B., Blank,M.L., Cabo,L.F.,
            Thompson,L.R., Burke,J.E., Boyle,J.P. and Boulanger,M.J.
  TITLE     Toxoplasma gondii mitochondrial association factor 1b interactome
            reveals novel binding partners including Ral GTPase accelerating
            protein alpha1
  JOURNAL   J Biol Chem 300 (1), 105582 (2024)
   PUBMED   38141762
  REMARK    GeneRIF: Toxoplasma gondii mitochondrial association factor 1b
            interactome reveals novel binding partners including Ral GTPase
            accelerating protein alpha1.
REFERENCE   2  (bases 1 to 9447)
  AUTHORS   Wagner,M., Skorobogatko,Y., Pode-Shakked,B., Powell,C.M.,
            Alhaddad,B., Seibt,A., Barel,O., Heimer,G., Hoffmann,C.,
            Demmer,L.A., Perilla-Young,Y., Remke,M., Wieczorek,D.,
            Navaratnarajah,T., Lichtner,P., Klee,D., Shamseldin,H.E., Al
            Mutairi,F., Mayatepek,E., Strom,T., Meitinger,T., Alkuraya,F.S.,
            Anikster,Y., Saltiel,A.R. and Distelmaier,F.
  TITLE     Bi-allelic Variants in RALGAPA1 Cause Profound Neurodevelopmental
            Disability, Muscular Hypotonia, Infantile Spasms, and Feeding
            Abnormalities
  JOURNAL   Am J Hum Genet 106 (2), 246-255 (2020)
   PUBMED   32004447
  REMARK    GeneRIF: Bi-allelic Variants in RALGAPA1 Cause Profound
            Neurodevelopmental Disability, Muscular Hypotonia, Infantile
            Spasms, and Feeding Abnormalities.
REFERENCE   3  (bases 1 to 9447)
  AUTHORS   Chen,Q., Quan,C., Xie,B., Chen,L., Zhou,S., Toth,R., Campbell,D.G.,
            Lu,S., Shirakawa,R., Horiuchi,H., Li,C., Yang,Z., MacKintosh,C.,
            Wang,H.Y. and Chen,S.
  TITLE     GARNL1, a major RalGAP alpha subunit in skeletal muscle, regulates
            insulin-stimulated RalA activation and GLUT4 trafficking via
            interaction with 14-3-3 proteins
  JOURNAL   Cell Signal 26 (8), 1636-1648 (2014)
   PUBMED   24768767
  REMARK    GeneRIF: GARNL1/RalGAPalpha1 is amajor RalGAPalphasubunit in
            skeletal muscle, and is regulated by protein phosphorylation and
            14-3-3 binding upon stimulation with insulin or muscle contraction.
REFERENCE   4  (bases 1 to 9447)
  AUTHORS   Shimojima,K., Komoike,Y., Tohyama,J., Takahashi,S., Paez,M.T.,
            Nakagawa,E., Goto,Y., Ohno,K., Ohtsu,M., Oguni,H., Osawa,M.,
            Higashinakagawa,T. and Yamamoto,T.
  TITLE     TULIP1 (RALGAPA1) haploinsufficiency with brain development delay
  JOURNAL   Genomics 94 (6), 414-422 (2009)
   PUBMED   19733229
  REMARK    GeneRIF: Reduced TULIP1 was found in lymphocytes from a
            developmentally delayed epileptic patient with a microdeletion of
            14q13.1q13.3 and in cell lysates from other patients with a
            missense P297 mutation.
REFERENCE   5  (bases 1 to 9447)
  AUTHORS   Shirakawa,R., Fukai,S., Kawato,M., Higashi,T., Kondo,H., Ikeda,T.,
            Nakayama,E., Okawa,K., Nureki,O., Kimura,T., Kita,T. and
            Horiuchi,H.
  TITLE     Tuberous sclerosis tumor suppressor complex-like complexes act as
            GTPase-activating proteins for Ral GTPases
  JOURNAL   J Biol Chem 284 (32), 21580-21588 (2009)
   PUBMED   19520869
REFERENCE   6  (bases 1 to 9447)
  AUTHORS   Schmid,E.M., Ford,M.G., Burtey,A., Praefcke,G.J., Peak-Chew,S.Y.,
            Mills,I.G., Benmerah,A. and McMahon,H.T.
  TITLE     Role of the AP2 beta-appendage hub in recruiting partners for
            clathrin-coated vesicle assembly
  JOURNAL   PLoS Biol 4 (9), e262 (2006)
   PUBMED   16903783
REFERENCE   7  (bases 1 to 9447)
  AUTHORS   Schwarzbraun,T., Vincent,J.B., Schumacher,A., Geschwind,D.H.,
            Oliveira,J., Windpassinger,C., Ofner,L., Ledinegg,M.K.,
            Kroisel,P.M., Wagner,K. and Petek,E.
  TITLE     Cloning, genomic structure, and expression profiles of TULIP1
            (GARNL1), a brain-expressed candidate gene for 14q13-linked
            neurological phenotypes, and its murine homologue
  JOURNAL   Genomics 84 (3), 577-586 (2004)
   PUBMED   15498464
REFERENCE   8  (bases 1 to 9447)
  AUTHORS   Fortna,A., Kim,Y., MacLaren,E., Marshall,K., Hahn,G., Meltesen,L.,
            Brenton,M., Hink,R., Burgers,S., Hernandez-Boussard,T.,
            Karimpour-Fard,A., Glueck,D., McGavran,L., Berry,R., Pollack,J. and
            Sikela,J.M.
  TITLE     Lineage-specific gene duplication and loss in human and great ape
            evolution
  JOURNAL   PLoS Biol 2 (7), E207 (2004)
   PUBMED   15252450
REFERENCE   9  (bases 1 to 9447)
  AUTHORS   Swaminathan,S., Kiendl,F., Korner,R., Lupetti,R., Hengst,L. and
            Melchior,F.
  TITLE     RanGAP1*SUMO1 is phosphorylated at the onset of mitosis and remains
            associated with RanBP2 upon NPC disassembly
  JOURNAL   J Cell Biol 164 (7), 965-971 (2004)
   PUBMED   15037602
  REMARK    GeneRIF: RanGAP1 remains associated with RanBP2/Nup358 and the SUMO
            E2-conjugating enzyme Ubc9 in mitosis.
REFERENCE   10 (bases 1 to 9447)
  AUTHORS   Heng,J.I. and Tan,S.S.
  TITLE     Cloning and characterization of GRIPE, a novel interacting partner
            of the transcription factor E12 in developing mouse forebrain
  JOURNAL   J Biol Chem 277 (45), 43152-43159 (2002)
   PUBMED   12200424
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL162311.5, AL137818.4,
            AL160231.4 and AY596970.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001346249.1.
            
            Summary: This gene encodes a major subunit of the RAL-GTPase
            activating protein. A similar protein in mouse binds E12, a
            transcriptional regulator of immunoglobulin genes. The mouse
            protein also functions in skeletal muscle by binding to the
            regulatory 14-3-3 proteins upon stimulation with insulin or muscle
            contraction. A pseudogene of this gene has been identified on
            chromosome 9. [provided by RefSeq, Oct 2016].
            
            Transcript Variant: This variant (11) contains an alternate exon in
            the coding region, and uses an alternate splice site in the coding
            region, but maintains the reading frame, compared to variant 1. The
            encoded isoform (8) is longer than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.51179.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000680220.1/ ENSP00000506280.1
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-566               AL162311.5         128801-129366
            567-677             AL162311.5         162351-162461
            678-727             AL162311.5         163041-163090
            728-758             AL162311.5         167097-167127
            759-785             AL137818.4         27480-27506
            786-829             AL137818.4         35695-35738
            830-1007            AL137818.4         37442-37619
            1008-1123           AL137818.4         41540-41655
            1124-1262           AL137818.4         46286-46424
            1263-1471           AL137818.4         47758-47966
            1472-1711           AL137818.4         49624-49863
            1712-1909           AL137818.4         55883-56080
            1910-2047           AL137818.4         59798-59935
            2048-2196           AL137818.4         69938-70086
            2197-2326           AL137818.4         73295-73424
            2327-2564           AL137818.4         75184-75421
            2565-2726           AL137818.4         76599-76760
            2727-2867           AL137818.4         98146-98286
            2868-4412           AL137818.4         108445-109989
            4413-4537           AL137818.4         111782-111906
            4538-4754           AL137818.4         113303-113519
            4755-4931           AL137818.4         114463-114639
            4932-5084           AL137818.4         120346-120498
            5085-5278           AL137818.4         123739-123932
            5279-5377           AL137818.4         124170-124268
            5378-5533           AL137818.4         125426-125581
            5534-5662           AL137818.4         126931-127059
            5663-5788           AL137818.4         133681-133806
            5789-5847           AL137818.4         139252-139310
            5848-5956           AL137818.4         142533-142641
            5957-6067           AL137818.4         143971-144081
            6068-6136           AL137818.4         146575-146643
            6137-6271           AL160231.4         192357-192491       c
            6272-6455           AL160231.4         191467-191650       c
            6456-7317           AL160231.4         183983-184844       c
            7318-7389           AL160231.4         182254-182325       c
            7390-7513           AL160231.4         162479-162602       c
            7514-7669           AL160231.4         152527-152682       c
            7670-7828           AL160231.4         129453-129611       c
            7829-7956           AL160231.4         127510-127637       c
            7957-8081           AL160231.4         106003-106127       c
            8082-8112           AL160231.4         105401-105431       c
            8113-8734           AL160231.4         95962-96583         c
            8735-9447           AY596970.1         7148-7860
FEATURES             Location/Qualifiers
     source          1..9447
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q13.2"
     gene            1..9447
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /note="Ral GTPase activating protein catalytic subunit
                     alpha 1"
                     /db_xref="GeneID:253959"
                     /db_xref="HGNC:HGNC:17770"
                     /db_xref="MIM:608884"
     exon            1..566
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             461..8089
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /note="isoform 8 is encoded by transcript variant 11;
                     GTPase activating Rap/RanGAP domain-like 1; GAP-related
                     interacting protein to E12; tuberin-like protein 1; Ral
                     GTPase activating protein, alpha subunit 1 (catalytic);
                     Ral GTPase activating protein catalytic alpha subunit 1"
                     /codon_start=1
                     /product="ral GTPase-activating protein subunit alpha-1
                     isoform 8"
                     /protein_id="NP_001333178.1"
                     /db_xref="CCDS:CCDS91864.1"
                     /db_xref="GeneID:253959"
                     /db_xref="HGNC:HGNC:17770"
                     /db_xref="MIM:608884"
                     /translation="MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLK
                     QFFDQHFSHIYYVFFENFVTIEASLKQKGHKSQREELDAILFIFEKILQLLPERIHQR
                     WQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQNNCSKEQLWMFSCLIPGFSA
                     PQSEHGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYFLEALLKYI
                     VIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPILDIPQMRPKP
                     HYVVIKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPK
                     NIQRAAASLVSREESKNDNADKTDRTTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLT
                     DIEIVRRVFSSKRSNVNFVTEIFRQAFLLPICEAAAMRKVVKVYQEWIQQEEKPLFMQ
                     EPEEIVITSSDLPCIENVTDHDISMEEGEKREEENGTNTADHVRNSSWAKNGSYQGAL
                     HNASEEATEQNIRAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKRILNIYR
                     YMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTL
                     IVAWIKANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDL
                     SDLPLDKLSEQKQKKHKGKGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGS
                     PGTEKARSIVRQKTVAMRSRSIGECALPSAYIRSAKSAPVLIHTSKPFLPDIVLTPLS
                     DELSDIDDAQILPRSTRVRHFSQSEETGNEVFGALNEEQPLPRSSSTSDILEPFTVER
                     AKGAVPVIDSSSRHAPSLQSSTEASSITRSTESHITDTHSRESSLEVGDSIYDHLCHL
                     IGPVELADSAFEQIQYIDLEGDDDLLSTLKEYFKENQENHSKNETGKDPASQEVTIAV
                     NRGERLSLDKLECTDQETESENITSFVGTPENLQFQKEPNSAVFMSNIAPNQSDSFFR
                     TQTSEKSKQLNTDKQPSEPSLDSPCDKEKRKHLYRQAATELDACVDVTLVEKLKSVQI
                     NEKITVPHVMRAKKATLKAPVNRRMPHVTSTSKLSPTKRSLSETVTHRAKIMKIATKK
                     RNSVHVTFRPSTESVQFYNPLENKEAPWKMRLRKLGGFSSGSSNSSTSNTHTSTNSAT
                     ELVKPGVYRPLDTLGTASVSSKTVKESTEIPTTILQKEGIASSQLGSRSTLRSSSHEA
                     GLQQGSLGGVYKTVVHALSKPKANVSPQRQNRMPPEAPLRDLYSHVMGYFGRKAAVNK
                     EDMSQKLPPLNSDIGGSSANVPDLMDEFIAERLRSGNASTMTRRGSSPGSLEIPKDLP
                     DILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSHSDSFSAFQYDGRKFDNFGFG
                     TDTGVTSSADVDSGSGHHQSAEEQEVASLTTLHIDSETSSLNQQAFSAEVATITGSES
                     ASPVHSPLGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCS
                     VMAGGTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDN
                     LGISTDNLTSPSPPVLIPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDV
                     SPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSLGLPGATMLIMDFIVAAGR
                     VASSAFLNAPRVEAQVLLGSLVCFPNLYCELPSLHPNIPDVAVSQFTDVKELIIKTVL
                     SSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNKTVAHVA
                     CNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLC
                     LLDWIMALPLKTLLQPFHATGAESDKTEKSVLNCIYKVLHGCVYGAQCFSNPRYFPMS
                     LSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQHGLISIAARTVI
                     THLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELFESPNIQFFVLNNTTLV
                     SCIQIRSEENMPGGGLSAGLASANSNVRIIVRDLSGKYSWDSAILYGPPPVSGLSEPT
                     SFMLSLSHQEKPEEPPTSNECLEDITVKDGLSLQFKRFRETVPTWDTIRDEEDVLDEL
                     LQYLGVTSPECLQRTGISLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFND
                     LNMKAVEQDEPIPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLD
                     SRQCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGL
                     QKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLGNDEVHIVWSEHTRD
                     YRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDGAIVNGKVLPIMVRA
                     TAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHLPS
                     DADH"
     exon            567..677
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            678..727
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            728..785
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            786..829
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            830..1007
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1123
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1124..1262
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1263..1471
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..1711
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1712..1909
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            1910..2047
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2048..2196
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2197..2326
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2327..2564
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2565..2726
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2727..2867
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            2868..4412
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4413..4537
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4538..4754
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4755..4931
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            4932..5084
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            5085..5278
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            5279..5377
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            5378..5533
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            5534..5662
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            5663..5788
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            5789..5847
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            5848..5956
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            5957..6067
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            6068..6136
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            6137..6271
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            6272..6455
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            6456..7317
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            7318..7389
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            7390..7513
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            7514..7669
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            7670..7828
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            7829..7956
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            7957..8081
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            8082..8112
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     exon            8113..9447
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9422..9427
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /note="hexamer: AATAAA"
     polyA_site      9447
                     /gene="RALGAPA1"
                     /gene_synonym="GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1;
                     TULIP1"
                     /note="major polyA site"
ORIGIN      
        1 acactcgtct ccgtttcggc ctctgcaggg tgtaagctgc taaagaagct tcaaccttaa
       61 gggggcctca gggagagagt tgtgctcctg ccgccgagag ctccaccttc gtggctctgg
      121 gtccggggcc ccggcctgtg gcggcgagcg gcggccgctg gagacgagcg gcggcggcag
      181 gcggccagcc cgggtctgcc tcgctccgtg cagggccggg agagggatcc gccatattgg
      241 agctggggcc gaaggtgcct tggagccgcg gcgcgaggcg ggtggaggtg gcggccctgg
      301 cctggatcgg gaaggagtcg gctctgccct ggcccgcaag gctggggagc taatgagcct
      361 ctctggctag tctcctcccc gtccactccc tccactttct gccgccggga cccggctggg
      421 agccggctgt ggcagtggca gtggcagtgg cagcgacagg atgttctcca agaagccgca
      481 cggggacgtg aagaagtcca cccagaaggt gctagacacc aagaaggacg cactgactcg
      541 cctcaagcac ctgcgcatcg tcatcgagaa tgcagaatct attgatctta aacagttttt
      601 cgaccaacat ttttcacata tatactatgt gttctttgaa aattttgtga ctattgaagc
      661 tagtcttaaa cagaaaggtc acaagtctca aagagaggaa ttggatgcta tactttttat
      721 ttttgagaaa attttacaac ttcttccaga aagaattcat cagcgatggc agtttcatag
      781 tattggattg attttaaaga agctacttca cacaggaaac tccttaaaga ttaggcggga
      841 aggtgttcgt cttttcttac tatggttgca agctcttcag aataactgta gcaaagaaca
      901 gctctggatg ttttcatgct taatccctgg attttcagca ccacagtctg aacatggacc
      961 tcgaacttta gataatctca ttaatcctcc actcaacctt caagaaactc aagtcactat
     1021 agaagaaatc actcctcttg tccccccaca atcaggagat aaagggcaag aagatctcac
     1081 aagctatttt cttgaagcac ttctaaaata catagtcatt caggtaaaaa gtttagaatg
     1141 gaagaacaaa gaaaaccaag aaaggggatt ttcatttttg ttttcacatt ttaagaaata
     1201 ttacttgcct tatatttttc caaacatctg taaggaaaac agtttatatc atcctatact
     1261 tgacatcccg cagatgagac caaagccaca ttatgtcgtg ataaagaaag atgctgaaac
     1321 caatgaagca atctattgta caaaggagcc tttcattaag gctcgtgtta ttgtcattcg
     1381 ttggctggtt tctttctggc tggagccaaa accacataca ggacctcata ttcctgggat
     1441 ggaaggtgaa gtcttgccaa agaatattca gagagcagct gctagtttag tatccagaga
     1501 agaaagcaaa aatgataatg ctgataaaac agacagaact acagaacccg aacagtctca
     1561 ttccaataca agcactctca cggagcgaga acctagctca tctagtctct gtagtattga
     1621 tgaagaacat ctcacagaca ttgaaatagt tcgcagagtt ttttcttcta aaaggagtaa
     1681 tgtaaacttt gtgacagaga tatttcgtca ggcattttta ttaccaattt gtgaagcagc
     1741 agctatgaga aaagtggtaa aagtatatca agaatggatc caacaagagg aaaaaccttt
     1801 gttcatgcaa gagcctgaag aaattgtgat cacttcttca gacctccctt gcattgaaaa
     1861 tgtcacagac catgatattt caatggaaga aggagaaaaa agagaagagg aaaatgggac
     1921 caatactgct gatcatgttc gaaattccag ttgggcaaaa aacggctcct accaaggtgc
     1981 tcttcataac gcctctgaag aagccacaga acaaaacata cgagctggta cccaggcagt
     2041 tttgcaggtg tttattataa actcatcaaa tatatttctt cttgaacctg caaatgaaat
     2101 aaaaaatctt ctggatgaac acacagatat gtgtaaacgc attcttaaca tttatcggta
     2161 catggttgta caagtatcaa tggacaaaaa gacttgggaa cagatgctgc ttgtgttgct
     2221 cagagtcacg gaatctgtac tgaagatgcc atcacaagct tttctacagt tccaagggaa
     2281 aaaaaatatg accttggcag gtcgacttgc aggaccactt ttccagaccc ttatagttgc
     2341 ctggatcaaa gcaaacctaa atgtgtacat ctcccgagaa ctttgggatg acttactgtc
     2401 agtattgtca tcgctgacct attgggaaga gttggccact gagtggtcac tgactatgga
     2461 gacattaact aaagttttag ctaggaattt atatagtttg gatctcagtg atttaccatt
     2521 ggataagctg agtgaacaga aacaaaaaaa gcacaaaggg aaaggagttg gacatgaatt
     2581 tcagaaagtt tcagttgaca agtcattttc tagaggatgg agtcgtgatc agcctggcca
     2641 agccccaatg agacagagga gtgcaacaac cactggttct ccaggaaccg aaaaggcgag
     2701 gagtatagta cggcaaaaaa ctgtcgccat gagaagccga tccattggtg aatgtgctct
     2761 gccatcggcc tatatacgca gtgctaaaag tgctcctgtt ctgatccata cttccaaacc
     2821 cttcttgcct gatattgttc tcactcccct ttctgatgag ctttcagata ttgatgatgc
     2881 tcaaatactt ccccgctcaa ctagagtcag acatttttca caaagtgaag aaactggaaa
     2941 tgaagttttt ggtgctttga atgaggagca gccattgcct cgaagtagca gcacttctga
     3001 catcttggaa ccattcactg ttgaacgagc caaaggtgca gttcctgtca ttgacagttc
     3061 atcccgtcat gcaccaagct tgcagagttc cacagaggct tcttcaataa ctagatccac
     3121 tgaaagccac atcactgata ctcatagtag agagtcttct ctggaagttg gtgatagcat
     3181 atatgaccat ctttgtcatt taataggtcc agtagaactt gcagattcag cttttgaaca
     3241 aatccagtac attgaccttg aaggagatga tgatcttctt tccaccctga aagaatattt
     3301 taaggaaaac caggaaaatc atagtaaaaa tgagacaggg aaagacccag cttctcagga
     3361 agtgactatt gcagtaaata ggggtgaaag attatcctta gataaattag aatgcacaga
     3421 tcaggaaact gaatcagaaa atatcacctc ttttgttggg actcctgaaa acctacagtt
     3481 tcagaaagaa ccaaactcag ctgtcttcat gagtaatatc gcacctaacc agtcagacag
     3541 tttttttaga acacaaactt ctgaaaaatc taagcaacta aacactgata aacagccatc
     3601 agagcctagt ttagatagcc cttgtgataa agaaaaaagg aaacatctgt acagacaagc
     3661 agccacagaa ttagatgctt gtgttgatgt aacactagtt gaaaagctta agagtgtgca
     3721 aattaatgaa aaaatcactg tcccacatgt tatgcgtgcc aagaaagcaa cactaaaagc
     3781 acctgttaac cgcagaatgc ctcatgttac aagtactagc aaactttctc caactaaaag
     3841 gagcttgtct gaaacagtaa ctcatagagc caaaatcatg aaaattgcta ctaaaaaacg
     3901 aaatagtgtt catgttactt ttaggccatc cactgagtcc gttcagtttt ataatcctct
     3961 ggaaaacaaa gaggctccat ggaagatgag actgcggaag ctcggtggct ttagtagtgg
     4021 tagcagcaat agcagcacta gcaacaccca taccagcaca aatagtgcta cagagctagt
     4081 aaaacctggt gtgtacagac ctctagatac acttggtact gcatcagtaa gcagcaaaac
     4141 agtgaaggaa tccacagaga ttcccaccac catattacaa aaagaaggaa ttgcaagtag
     4201 tcaattaggt agtcgtagta ctcttaggtc atcaagtcat gaggcaggac tacagcaggg
     4261 ctccctgggt ggcgtttata aaactgttgt acatgctctt tcgaagccga aggcaaatgt
     4321 ttccccacag aggcagaaca gaatgccacc agaggctcca ctgagggatc tgtacagtca
     4381 tgtaatgggc tattttggaa ggaaagctgc agtcaataaa gaggacatga gccaaaaact
     4441 gcctcctctt aatagtgata ttggcggcag cagtgctaat gttcctgatc tgatggatga
     4501 gtttatagca gaacgacttc gaagtggtaa tgcctcgact atgacaagaa gaggaagtag
     4561 tccaggcagc ctggaaattc ccaaagacct ccctgatatt ctaaacaagc agaaccagat
     4621 gcgccctatt gatgacccag gtgtgccctc agaatggact tctcctgcca gtgcagggag
     4681 cagtgatctt atcagctcag atagtcattc ggattctttc agcgctttcc aatatgatgg
     4741 ccgaaaattt gacaattttg gctttggaac cgacactggg gttacgtcct ctgctgatgt
     4801 ggattcaggt tctggccatc atcagagtgc tgaagagcag gaagtggcta gtctaactac
     4861 tcttcatata gattctgaaa caagcagtct taatcagcaa gctttctctg ctgaagttgc
     4921 aactattact ggttcagaaa gtgcttcccc agtccactca cctctgggct ccaggtcaca
     4981 gactccttcc ccttctacat tgaatataga tcacatggaa cagaaggatc tgcagctcga
     5041 cgagaagctc caccactctg ttcttcagac accagatgat ctagaaatta gtgaatttcc
     5101 atcagaatgt tgtagtgtga tggcaggagg tactctgact ggatggcatg ctgatgttgc
     5161 tactgtaatg tggcgaagaa tgctaggcat tttgggagat gtaaattcaa tcatggatcc
     5221 tgaaatacat gctcaagttt ttgattacct ctgtgaactt tggcagaatc tagctaagat
     5281 tagagataac cttggcattt caactgataa cctgacctcc ccttctccac cagttttaat
     5341 tcctccactg agaattctta caccttggct ttttaaggca accatgttga ctgataaata
     5401 taaacaaggt aaattacatg catataaact tatttgtaat acaatgaaaa gaagacaaga
     5461 tgtttctcca aatagagatt ttctaacaca tttctacaat ataatgcatt gtggattact
     5521 tcatattgac caggatattg tcaatacaat catcaaacac tgctcacctc aatttttttc
     5581 acttggtttg cctggtgcca caatgcttat tatggatttt attgtagcag ctggtagagt
     5641 ggcttcttca gcttttctca atgcaccaag agtagaagca caagttcttc tgggatcttt
     5701 ggtttgcttt cccaacttat attgtgaact gccttctctt catcccaaca ttcctgatgt
     5761 tgctgtgtct cagtttacag atgttaagga acttataatc aaaactgtat taagctcggc
     5821 aagagatgag ccctctggtc ctgcacgatg tgtagcactt tgtagtttag gtatttggat
     5881 ttgtgaagaa ctagtccatg agtctcatca tcctcaaatt aaggaagctc tgaatgtgat
     5941 ttgtgtttcc ttaaagttta ctaataaaac agtagcccac gtagcttgta acatgcttca
     6001 catgctggtt cattatgtac ctagacttca gatttaccag cctgattctc ccttgaaaat
     6061 tattcaaatc ctaatagcta ccatcaccca tcttttacca agtacagagg cttcatctta
     6121 tgaaatggac aagaggttgg tagtatcttt acttctctgc cttctggact ggatcatggc
     6181 cttacctcta aagacactgc tccaaccatt tcatgctacg ggagcagaaa gcgataaaac
     6241 agaaaaatct gttctcaatt gcatttataa ggttttacat gggtgtgttt atggagctca
     6301 gtgttttagc aatccaaggt attttcccat gagcctctct gatttggcat ctgtagatta
     6361 tgatcctttt atgcatttgg aaagtctgaa agagcctgag cctctgcact ctcctgactc
     6421 agaacgatct tctaaactcc agccagtaac agaagtgaaa actcaaatgc agcatggatt
     6481 aatctctata gcagcccgca ctgttattac acatctggta aatcacctgg gccattatcc
     6541 aatgagcggt ggtcctgcta tgctaacaag tcaggtgtgt gaaaatcacg acaatcatta
     6601 cagtgaaagt actgaacttt ctcctgaact ctttgagagt ccaaatatcc agttctttgt
     6661 gttaaataat acaaccttag tgtcctgtat ccagatcaga tcagaagaga atatgcctgg
     6721 aggaggttta tctgctggcc ttgcatcagc caattcaaat gtcagaatca tagtacgtga
     6781 tctctctgga aaatattcat gggattctgc tatactgtat ggcccacctc ctgtaagtgg
     6841 cttgtcagaa cctacatctt tcatgctttc attgtctcac caagagaagc cagaagagcc
     6901 tccgacatct aatgaatgct tagaagatat aaccgtaaaa gatggacttt ctctccagtt
     6961 taaaagattt agagaaactg taccaacttg ggatacaata agagatgaag aagatgttct
     7021 tgatgagctc ttgcagtatt tgggtgttac tagtcctgaa tgcttacaga gaactggaat
     7081 ctcacttaat attcctgctc cacaacctgt gtgcatttct gaaaaacaag aaaatgatgt
     7141 tattaatgct atccttaagc aacatacaga agaaaaagaa tttgttgaga agcactttaa
     7201 tgacttaaac atgaaagctg tggaacaaga tgaaccaata cctcaaaaac ctcagtcagc
     7261 attttattat tgcagattgc ttcttagtat attgggaatg aattcctggg acaaacggag
     7321 gagctttcat ctcctgaaga aaaatgaaaa gctacttaga gaacttagga acttggattc
     7381 aaggcagtgc cgagagacac acaagattgc agtattttat gttgctgaag gacaagaaga
     7441 caaacactcc attctcacca atacaggagg aagtcaagca tatgaagatt ttgtagctgg
     7501 tcttggttgg gaggtaaatc ttacaaacca ttgtggtttt atgggaggac tacaaaaaaa
     7561 caaaagcact ggattgacca ctccatattt tgctacctct acagtagagg taatatttca
     7621 cgtgtcaaca agaatgcctt ctgattctga tgattctttg accaaaaaat tgagacattt
     7681 gggaaatgat gaagtgcaca ttgtttggtc agagcatact agagactaca ggagaggaat
     7741 tattcccaca gaatttggtg atgtccttat tgtaatatat ccaatgaaaa atcacatgtt
     7801 cagtattcag ataatgaaaa aaccagaggt tcccttcttt ggtccccttt ttgatggtgc
     7861 tattgtgaat ggaaaggttc tacccattat ggttagagca acagctataa atgcaagccg
     7921 tgctctgaaa tctctgattc cattgtatca aaacttctat gaggagagag cacgatacct
     7981 gcaaacaatt gtccagcacc acttagaacc aacaacattt gaagattttg cagcacaggt
     8041 tttttctcca gctccctacc accatttacc atctgatgcc gatcattaaa tatcagttct
     8101 gtttatctga aggctcctac ccagagattc tacccagtga aactcccaca gcaacgcagg
     8161 tagatggggc tgacctggcc tctccaatgt ctcctcgaac tagcaaaagc cgcatgtcca
     8221 tgaagctgcg tcgttcctct ggctcagcca ataaatccta aggagacaag cagcccagca
     8281 gtgatcagca gtagccacct tagcacgaac atagggttaa ccctttcagg ccttcatgtc
     8341 tgccataaca tgcatgtttc ttcctgtaca tttatttgag aaaacactgg atttaaataa
     8401 ttttaaataa tttgtagctt aatattaaag atttaagtta tttattgttt catttttttt
     8461 cccacaatcc aagctgccat attttgaggg cagggggagt tttattctac accctttacc
     8521 ttcctagata attatgtcta agtagtttta tctttaattt catggttaac tgtgagccaa
     8581 aatacaattg gacaattagt ctcattattt attgtgcccc attgcaactt tatggttcaa
     8641 taaatatata attttttaca aatgtaaaat tttacattta agcatttgta aagttacagc
     8701 aaaagatgta cctgttaata cacagaatgt gtacagatta tttgttatga caataaaaca
     8761 ctcaaaataa atggtcttta gcatctcaaa ttccaactga aatcatttta gtattaactc
     8821 ttcttcccaa agcaatgtct catttcttgg ctgtgcaggt gatgccatgt tatatccaat
     8881 aactagaaaa atcactgtgc tgaactttta tgtttagctt ccaagtattt ttctaatgtt
     8941 ttgcatttca agtggtatca ctgttaaatg ccatttgttt tcagattgtg gccttttatt
     9001 attggctgct agatcctggt gtttctatgt tcttttttaa gcaccaaaaa gaagatgggg
     9061 aagaaaagaa ggaaaatttt ctgatataaa tatgttgttc aaattatgag tattatttaa
     9121 aaaagaaaaa ggaacataac ccaggagtct aagttaaatc taatattgtt aatactgaac
     9181 ttgcaggtcc aggttggtat acattccacc ctctagaagt attttcttac agtagataag
     9241 ctgctcacat tttgttttga atgggcatct cctgaggaaa tgtagcatga cattggtact
     9301 aactgcatgt gtaaatacat catactggca aaccgtaaaa tataaattat gtatcatcat
     9361 tcatgtagta tctataattt gtaacagtgg gggggaaaga tgacatggta tttaataata
     9421 caataaaaat attcttatca cttccta
//
