LOCUS       NM_000448               6588 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens recombination activating 1 (RAG1), transcript variant
            1, mRNA.
ACCESSION   NM_000448
VERSION     NM_000448.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Zhang,J., Quintal,L., Atkinson,A., Williams,B., Grunebaum,E. and
            Roifman,C.M.
  TITLE     Novel RAG1 mutation in a case of severe combined immunodeficiency
  JOURNAL   Pediatrics 116 (3), e445-e449 (2005)
   PUBMED   16061569
  REMARK    GeneRIF: Novel RAG1 mutation in a case of severe combined
            immunodeficiency. A novel homozygous thymine to cytosine
            substitution at nucleotide position 2686
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Noordzij,J.G., de Bruin-Versteeg,S., Verkaik,N.S., Vossen,J.M., de
            Groot,R., Bernatowska,E., Langerak,A.W., van Gent,D.C. and van
            Dongen,J.J.
  TITLE     The immunophenotypic and immunogenotypic B-cell differentiation
            arrest in bone marrow of RAG-deficient SCID patients corresponds to
            residual recombination activities of mutated RAG proteins
  JOURNAL   Blood 100 (6), 2145-2152 (2002)
   PUBMED   12200379
  REMARK    GeneRIF: B-cell differentiation arrest in bone marrow of
            RAG-deficient SCID patients corresponds to residual recombination
            activities of mutated RAG1 and RAG2 proteins.
REFERENCE   3  (bases 1 to 6588)
  AUTHORS   Corneo,B., Moshous,D., Gungor,T., Wulffraat,N., Philippet,P., Le
            Deist,F.L., Fischer,A. and de Villartay,J.P.
  TITLE     Identical mutations in RAG1 or RAG2 genes leading to defective
            V(D)J recombinase activity can cause either T-B-severe combined
            immune deficiency or Omenn syndrome
  JOURNAL   Blood 97 (9), 2772-2776 (2001)
   PUBMED   11313270
REFERENCE   4  (bases 1 to 6588)
  AUTHORS   Villa,A., Sobacchi,C., Notarangelo,L.D., Bozzi,F., Abinun,M.,
            Abrahamsen,T.G., Arkwright,P.D., Baniyash,M., Brooks,E.G.,
            Conley,M.E., Cortes,P., Duse,M., Fasth,A., Filipovich,A.M.,
            Infante,A.J., Jones,A., Mazzolari,E., Muller,S.M., Pasic,S.,
            Rechavi,G., Sacco,M.G., Santagata,S., Schroeder,M.L., Seger,R.,
            Strina,D., Ugazio,A., Valiaho,J., Vihinen,M., Vogler,L.B., Ochs,H.,
            Vezzoni,P., Friedrich,W. and Schwarz,K.
  TITLE     V(D)J recombination defects in lymphocytes due to RAG mutations:
            severe immunodeficiency with a spectrum of clinical presentations
  JOURNAL   Blood 97 (1), 81-88 (2001)
   PUBMED   11133745
REFERENCE   5  (bases 1 to 6588)
  AUTHORS   Santagata,S., Gomez,C.A., Sobacchi,C., Bozzi,F., Abinun,M.,
            Pasic,S., Cortes,P., Vezzoni,P. and Villa,A.
  TITLE     N-terminal RAG1 frameshift mutations in Omenn's syndrome: internal
            methionine usage leads to partial V(D)J recombination activity and
            reveals a fundamental role in vivo for the N-terminal domains
  JOURNAL   Proc Natl Acad Sci U S A 97 (26), 14572-14577 (2000)
   PUBMED   11121059
REFERENCE   6  (bases 1 to 6588)
  AUTHORS   Noordzij,J.G., Verkaik,N.S., Hartwig,N.G., de Groot,R., van
            Gent,D.C. and van Dongen,J.J.
  TITLE     N-terminal truncated human RAG1 proteins can direct T-cell receptor
            but not immunoglobulin gene rearrangements
  JOURNAL   Blood 96 (1), 203-209 (2000)
   PUBMED   10891452
REFERENCE   7  (bases 1 to 6588)
  AUTHORS   Villa,A., Bozzi,F., Sobacchi,C., Strina,D., Fasth,A., Pasic,S.,
            Notarangelo,L.D. and Vezzoni,P.
  TITLE     Prenatal diagnosis of RAG-deficient Omenn syndrome
  JOURNAL   Prenat Diagn 20 (1), 56-59 (2000)
   PUBMED   10701853
REFERENCE   8  (bases 1 to 6588)
  AUTHORS   Wada,T., Takei,K., Kudo,M., Shimura,S., Kasahara,Y., Koizumi,S.,
            Kawa-Ha,K., Ishida,Y., Imashuku,S., Seki,H. and Yachie,A.
  TITLE     Characterization of immune function and analysis of RAG gene
            mutations in Omenn syndrome and related disorders
  JOURNAL   Clin Exp Immunol 119 (1), 148-155 (2000)
   PUBMED   10606976
REFERENCE   9  (bases 1 to 6588)
  AUTHORS   Kumaki,S., Ishii,N., Minegishi,M., Tsuchiya,S., Cosman,D.,
            Sugamura,K. and Konno,T.
  TITLE     Functional role of interleukin-4 (IL-4) and IL-7 in the development
            of X-linked severe combined immunodeficiency
  JOURNAL   Blood 93 (2), 607-612 (1999)
   PUBMED   9885222
REFERENCE   10 (bases 1 to 6588)
  AUTHORS   Villa,A., Santagata,S., Bozzi,F., Giliani,S., Frattini,A.,
            Imberti,L., Gatta,L.B., Ochs,H.D., Schwarz,K., Notarangelo,L.D.,
            Vezzoni,P. and Spanopoulou,E.
  TITLE     Partial V(D)J recombination activity leads to Omenn syndrome
  JOURNAL   Cell 93 (5), 885-896 (1998)
   PUBMED   9630231
REFERENCE   11 (bases 1 to 6588)
  AUTHORS   Brugnoni,D., Airo,P., Facchetti,F., Blanzuoli,L., Ugazio,A.G.,
            Cattaneo,R. and Notarangelo,L.D.
  TITLE     In vitro cell death of activated lymphocytes in Omenn's syndrome
  JOURNAL   Eur J Immunol 27 (11), 2765-2773 (1997)
   PUBMED   9394797
REFERENCE   12 (bases 1 to 6588)
  AUTHORS   Schwarz,K., Gauss,G.H., Ludwig,L., Pannicke,U., Li,Z., Lindner,D.,
            Friedrich,W., Seger,R.A., Hansen-Hagge,T.E., Desiderio,S.,
            Lieber,M.R. and Bartram,C.R.
  TITLE     RAG mutations in human B cell-negative SCID
  JOURNAL   Science 274 (5284), 97-99 (1996)
   PUBMED   8810255
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC139427.3 and M29474.1.
            
            On Dec 24, 2019 this sequence version replaced NM_000448.2.
            
            Summary: The protein encoded by this gene is involved in activation
            of immunoglobulin V-D-J recombination. The encoded protein is
            involved in recognition of the DNA substrate, but stable binding
            and cleavage activity also requires RAG2. Defects in this gene can
            be the cause of several diseases. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: Variants 1 through 5 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single transcript was available
            for the full length of the gene. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: M29474.1, SRR14038196.2709684.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142853, SAMEA2144120
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000299440.6/ ENSP00000299440.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                AC139427.3         133046-133063       c
            19-875              M29474.1           1-857
            876-876             AC139427.3         127020-127020       c
            877-3615            M29474.1           859-3597
            3616-6588           AC139427.3         121308-124280       c
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11p12"
     gene            1..6588
                     /gene="RAG1"
                     /gene_synonym="RAG-1; RNF74"
                     /note="recombination activating 1"
                     /db_xref="GeneID:5896"
                     /db_xref="HGNC:HGNC:9831"
                     /db_xref="MIM:179615"
     exon            1..116
                     /gene="RAG1"
                     /gene_synonym="RAG-1; RNF74"
                     /inference="alignment:Splign:2.1.0"
     exon            117..6588
                     /gene="RAG1"
                     /gene_synonym="RAG-1; RNF74"
                     /inference="alignment:Splign:2.1.0"
     CDS             131..3262
                     /gene="RAG1"
                     /gene_synonym="RAG-1; RNF74"
                     /EC_number="2.3.2.27"
                     /note="RING finger protein 74; recombination activating
                     protein 1; recombination activating gene 1"
                     /codon_start=1
                     /product="V(D)J recombination-activating protein 1"
                     /protein_id="NP_000439.2"
                     /db_xref="CCDS:CCDS7902.1"
                     /db_xref="GeneID:5896"
                     /db_xref="HGNC:HGNC:9831"
                     /db_xref="MIM:179615"
                     /translation="MAASFPPTLGLSSAPDEIQHPHIKFSEWKFKLFRVRSFEKTPEE
                     AQKEKKDSFEGKPSLEQSPAVLDKADGQKPVPTQPLLKAHPKFSKKFHDNEKARGKAI
                     HQANLRHLCRICGNSFRADEHNRRYPVHGPVDGKTLGLLRKKEKRATSWPDLIAKVFR
                     IDVKADVDSIHPTEFCHNCWSIMHRKFSSAPCEVYFPRNVTMEWHPHTPSCDICNTAR
                     RGLKRKSLQPNLQLSKKLKTVLDQARQARQHKRRAQARISSKDVMKKIANCSKIHLST
                     KLLAVDFPEHFVKSISCQICEHILADPVETNCKHVFCRVCILRCLKVMGSYCPSCRYP
                     CFPTDLESPVKSFLSVLNSLMVKCPAKECNEEVSLEKYNHHISSHKESKEIFVHINKG
                     GRPRQHLLSLTRRAQKHRLRELKLQVKAFADKEEGGDVKSVCMTLFLLALRARNEHRQ
                     ADELEAIMQGKGSGLQPAVCLAIRVNTFLSCSQYHKMYRTVKAITGRQIFQPLHALRN
                     AEKVLLPGYHHFEWQPPLKNVSSSTDVGIIDGLSGLSSSVDDYPVDTIAKRFRYDSAL
                     VSALMDMEEDILEGMRSQDLDDYLNGPFTVVVKESCDGMGDVSEKHGSGPVVPEKAVR
                     FSFTIMKITIAHSSQNVKVFEEAKPNSELCCKPLCLMLADESDHETLTAILSPLIAER
                     EAMKSSELMLELGGILRTFKFIFRGTGYDEKLVREVEGLEASGSVYICTLCDATRLEA
                     SQNLVFHSITRSHAENLERYEVWRSNPYHESVEELRDRVKGVSAKPFIETVPSIDALH
                     CDIGNAAEFYKIFQLEIGEVYKNPNASKEERKRWQATLDKHLRKKMNLKPIMRMNGNF
                     ARKLMTKETVDAVCELIPSEERHEALRELMDLYLKMKPVWRSSCPAKECPESLCQYSF
                     NSQRFAELLSTKFKYRYEGKITNYFHKTLAHVPEIIERDGSIGAWASEGNESGNKLFR
                     RFRKMNARQSKCYEMEDVLKHHWLYTSKYLQKFMNAHNALKTSGFTMNPQASLGDPLG
                     IEDSLESQDSMEF"
     regulatory      6566..6571
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAG1"
                     /gene_synonym="RAG-1; RNF74"
                     /note="hexamer: AATAAA"
     polyA_site      6588
                     /gene="RAG1"
                     /gene_synonym="RAG-1; RNF74"
                     /note="major polyA site"
ORIGIN      
        1 gaaacaagag ggcaaggaga gagcagagaa cacactttgc cttctctttg gtattgagta
       61 atatcaacca aattgcagac atctcaacac tttggccagg cagcctgctg agcaaggtac
      121 ctcagccagc atggcagcct ctttcccacc caccttggga ctcagttctg ccccagatga
      181 aattcagcac ccacatatta aattttcaga atggaaattt aagctgttcc gggtgagatc
      241 ctttgaaaag acacctgaag aagctcaaaa ggaaaagaag gattcctttg aggggaaacc
      301 ctctctggag caatctccag cagtcctgga caaggctgat ggtcagaagc cagtcccaac
      361 tcagccattg ttaaaagccc accctaagtt ttcaaagaaa tttcacgaca acgagaaagc
      421 aagaggcaaa gcgatccatc aagccaacct tcgacatctc tgccgcatct gtgggaattc
      481 ttttagagct gatgagcaca acaggagata tccagtccat ggtcctgtgg atggtaaaac
      541 cctaggcctt ttacgaaaga aggaaaagag agctacttcc tggccggacc tcattgccaa
      601 ggttttccgg atcgatgtga aggcagatgt tgactcgatc caccccactg agttctgcca
      661 taactgctgg agcatcatgc acaggaagtt tagcagtgcc ccatgtgagg tttacttccc
      721 gaggaacgtg accatggagt ggcaccccca cacaccatcc tgtgacatct gcaacactgc
      781 ccgtcgggga ctcaagagga agagtcttca gccaaacttg cagctcagca aaaaactcaa
      841 aactgtgctt gaccaagcaa gacaagcccg tcagcacaag agaagagctc aggcaaggat
      901 cagcagcaag gatgtcatga agaagatcgc caactgcagt aagatacatc ttagtaccaa
      961 gctccttgca gtggacttcc cagagcactt tgtgaaatcc atctcctgcc agatctgtga
     1021 acacattctg gctgaccctg tggagaccaa ctgtaagcat gtcttttgcc gggtctgcat
     1081 tctcagatgc ctcaaagtca tgggcagcta ttgtccctct tgccgatatc catgcttccc
     1141 tactgacctg gagagtccag tgaagtcctt tctgagcgtc ttgaattccc tgatggtgaa
     1201 atgtccagca aaagagtgca atgaggaggt cagtttggaa aaatataatc accacatctc
     1261 aagtcacaag gaatcaaaag agatttttgt gcacattaat aaagggggcc ggccccgcca
     1321 acatcttctg tcgctgactc ggagagctca gaagcaccgg ctgagggagc tcaagctgca
     1381 agtcaaagcc tttgctgaca aagaagaagg tggagatgtg aagtccgtgt gcatgacctt
     1441 gttcctgctg gctctgaggg cgaggaatga gcacaggcaa gctgatgagc tggaggccat
     1501 catgcaggga aagggctctg gcctgcagcc agctgtttgc ttggccatcc gtgtcaacac
     1561 cttcctcagc tgcagtcagt accacaagat gtacaggact gtgaaagcca tcacagggag
     1621 acagattttt cagcctttgc atgcccttcg gaatgctgag aaggtacttc tgccaggcta
     1681 ccaccacttt gagtggcagc cacctctgaa gaatgtgtct tccagcactg atgttggcat
     1741 tattgatggg ctgtctggac tatcatcctc tgtggatgat tacccagtgg acaccattgc
     1801 aaagaggttc cgctatgatt cagctttggt gtctgctttg atggacatgg aagaagacat
     1861 cttggaaggc atgagatccc aagaccttga tgattacctg aatggcccct tcactgtggt
     1921 ggtgaaggag tcttgtgatg gaatgggaga cgtgagtgag aagcatggga gtgggcctgt
     1981 agttccagaa aaggcagtcc gtttttcatt cacaatcatg aaaattacta ttgcccacag
     2041 ctctcagaat gtgaaagtat ttgaagaagc caaacctaac tctgaactgt gttgcaagcc
     2101 attgtgcctt atgctggcag atgagtctga ccacgagacg ctgactgcca tcctgagtcc
     2161 tctcattgct gagagggagg ccatgaagag cagtgaatta atgcttgagc tgggaggcat
     2221 tctccggact ttcaagttca tcttcagggg caccggctat gatgaaaaac ttgtgcggga
     2281 agtggaaggc ctcgaggctt ctggctcagt ctacatttgt actctttgtg atgccacccg
     2341 tctggaagcc tctcaaaatc ttgtcttcca ctctataacc agaagccatg ctgagaacct
     2401 ggaacgttat gaggtctggc gttccaaccc ttaccatgag tctgtggaag aactgcggga
     2461 tcgggtgaaa ggggtctcag ctaaaccttt cattgagaca gtcccttcca tagatgcact
     2521 ccactgtgac attggcaatg cagctgagtt ctacaagatc ttccagctag agatagggga
     2581 agtgtataag aatcccaatg cttccaaaga ggaaaggaaa aggtggcagg ccacactgga
     2641 caagcatctc cggaagaaga tgaacctcaa accaatcatg aggatgaatg gcaactttgc
     2701 caggaagctc atgaccaaag agactgtgga tgcagtttgt gagttaattc cttccgagga
     2761 gaggcacgag gctctgaggg agctgatgga tctttacctg aagatgaaac cagtatggcg
     2821 atcatcatgc cctgctaaag agtgcccaga atccctctgc cagtacagtt tcaattcaca
     2881 gcgttttgct gagctccttt ctacgaagtt caagtatagg tatgagggaa aaatcaccaa
     2941 ttattttcac aaaaccctgg cccatgttcc tgaaattatt gagagggatg gctccattgg
     3001 ggcatgggca agtgagggaa atgagtctgg taacaaactg tttaggcgct tccggaaaat
     3061 gaatgccagg cagtccaaat gctatgagat ggaagatgtc ctgaaacacc actggttgta
     3121 cacctccaaa tacctccaga agtttatgaa tgctcataat gcattaaaaa cctctgggtt
     3181 taccatgaac cctcaggcaa gcttagggga cccattaggc atagaggact ctctggaaag
     3241 ccaagattca atggaatttt aagtagggca accacttatg agttggtttt tgcaattgag
     3301 tttccctctg ggttgcattg agggcttctc ctagcaccct ttactgctgt gtatggggct
     3361 tcaccatcca agaggtggta ggttggagta agatgctaca gatgctctca agtcaggaat
     3421 agaaactgat gagctgattg cttgaggctt ttagtgagtt ccgaaaagca acaggaaaaa
     3481 tcagttatct gaaagctcag taactcagaa caggagtaac tgcaggggac cagagatgag
     3541 caaagatctg tgtgtgttgg ggagctgtca tgtaaatcaa agccaaggtt gtcaaagaac
     3601 agccagtgag gccaggaaag aaattggtct tgtggttttc atttttttcc cccttgattg
     3661 attatatttt gtattgagat atgataagtg ccttctattt catttttgaa taattcttca
     3721 tttttataat tttacatatc ttggcttgct atataagatt caaaagagct ttttaaattt
     3781 ttctaataat atcttacatt tgtacagcat gatgaccttt acaaagtgct ctcaatgcat
     3841 ttacccattc gttatataaa tatgttacat caggacaact ttgagaaaat cagtcctttt
     3901 ttatgtttaa attatgtatc tattgtaacc ttcagagttt aggaggtcat ctgctgtcat
     3961 ggatttttca ataatgaatt tagaatacac ctgttagcta cagttagtta ttaaatcttc
     4021 tgataatata tgtttactta gctatcagaa gccaagtatg attctttatt tttacttttt
     4081 catttcaaga aatttagagt ttccaaattt agagcttctg catacagtct taaagccaca
     4141 gaggcttgta aaaatatagg ttagcttgat gtctaaaaat atatttcatg tcttactgaa
     4201 acattttgcc agactttctc caaatgaaac ctgaatcaat ttttctaaat ctaggtttca
     4261 tagagtcctc tcctctgcaa tgtgttattc tttctataat gatcagttta ctttcagtgg
     4321 attcagaatt gtgtagcagg ataaccttgt atttttccat ccgctaagtt tagatggagt
     4381 ccaaacgcag tacagcagaa gagttaacat ttacacagtg ctttttacca ctgtggaatg
     4441 ttttcacact catttttcct tacaacaatt ctgaggagta ggtgttgtta ttatctccat
     4501 ttgatggggg tttaaatgat ttgctcaaag tcatttaggg gtaataaata cttggcttgg
     4561 aaatttaaca cagtcctttt gtctccaaag cccttcttct ttccaccaca aattaatcac
     4621 tatgtttata aggtagtatc agaatttttt taggattcac aactaatcac tatagcacat
     4681 gaccttggga ttacattttt atggggcagg ggtaagcaag tttttaaatc atttgtgtgc
     4741 tctggctctt ttgatagaag aaagcaacac aaaagctcca aagggccccc taaccctctt
     4801 gtggctccag ttatttggaa actatgatct gcatccttag gaatctggga tttgccagtt
     4861 gctggcaatg tagagcaggc atggaatttt atatgctagt gagtcataat gatatgttag
     4921 tgttaattag ttttttcttc ctttgatttt attggccata attgctactc ttcatacaca
     4981 gtatatcaaa gagcttgata atttagttgt caaaagtgca tcggcgacat tatctttaat
     5041 tgtatgtatt tggtgcttct tcagggattg aactcagtat ctttcattaa aaaacacagc
     5101 agttttcctt gctttttata tgcagaatat caaagtcatt tctaatttag ttgtcaaaaa
     5161 catatacata ttttaacatt agtttttttg aaaactcttg gttttgtttt tttggaaatg
     5221 agtgggccac taagccacac tttcccttca tcctgcttaa tccttccagc atgtctctgc
     5281 actaataaac agctaaattc acataatcat cctatttact gaagcatggt catgctggtt
     5341 tatagatttt ttacccattt ctactctttt tctctattgg tggcactgta aatactttcc
     5401 agtattaaat tatccttttc taacactgta ggaactattt tgaatgcatg tgactaagag
     5461 catgatttat agcacaacct ttccaataat cccttaatca gatcacattt tgataaaccc
     5521 tgggaacatc tggctgcagg aatttcaata tgtagaaacg ctgcctatgg ttttttgccc
     5581 ttactgttga gactgcaata tcctagaccc tagttttata ctagagtttt atttttagca
     5641 atgcctattg caagtgcaat tatatactcc agggaaattc accacactga atcgagcatt
     5701 tgtgtgtgta tgtgtgaagt atatactggg acttcagaag tgcaatgtat ttttctcctg
     5761 tgaaacctga atctacaagt tttcctgcca agccactcag gtgcattgca gggaccagtg
     5821 ataatggctg atgaaaattg atgattggtc agtgaggtca aaaggagcct tgggattaat
     5881 aaacatgcac tgagaagcaa gaggaggaga aaaagatgtc tttttcttcc aggtgaactg
     5941 gaatttagtt ttgcctcaga tttttttccc acaagataca gaagaagata aagatttttt
     6001 tggttgagag tgtgggtctt gcattacatc aaacagagtt caaattccac acagataaga
     6061 ggcaggatat ataagcgcca gtggtagttg ggaggaataa accattattt ggatgcaggt
     6121 ggtttttgat tgcaaatatg tgtgtgtctt cagtgattgt atgacagatg atgtattctt
     6181 ttgatgttaa aagattttaa gtaagagtag atacattgta cccattttac attttcttat
     6241 tttaactaca gtaatctaca taaatatacc tcagaaatca tttttggtga ttattttttg
     6301 ttttgtagaa ttgcacttca gtttattttc ttacaaataa ccttacattt tgtttaatgg
     6361 cttccaagag cctttttttt ttttgtattt cagagaaaat tcaggtacca ggatgcaatg
     6421 gatttatttg attcagggga cctgtgtttc catgtcaaat gttttcaaat aaaatgaaat
     6481 atgagtttca atacttttta tattttaata tttccattca ttaatattat ggttattgtc
     6541 agcaatttta tgtttgaata tttgaaataa aagtttaaga tttgaaaa
//
