LOCUS       NM_002880               3191 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1),
            transcript variant 2, mRNA.
ACCESSION   NM_002880
VERSION     NM_002880.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Khoroshkin,M., Buyan,A., Dodel,M., Navickas,A., Yu,J., Trejo,F.,
            Doty,A., Baratam,R., Zhou,S., Lee,S.B., Joshi,T., Garcia,K.,
            Choi,B., Miglani,S., Subramanyam,V., Modi,H., Carpenter,C.,
            Markett,D., Corces,M.R., Mardakheh,F.K., Kulakovskiy,I.V. and
            Goodarzi,H.
  TITLE     Systematic identification of post-transcriptional regulatory
            modules
  JOURNAL   Nat Commun 15 (1), 7872 (2024)
   PUBMED   39251607
  REMARK    Erratum:[Nat Commun. 2024 Oct 28;15(1):9277. doi:
            10.1038/s41467-024-52903-4.. PMID: 39468017]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Motta,M., Barresi,S., Pizzi,S., Bifano,D., Lopez Marti,J.,
            Garrido-Pontnou,M., Flex,E., Bruselles,A., Giovannoni,I.,
            Rotundo,G., Fragale,A., Tirelli,V., Vallese,S., Ciolfi,A.,
            Bisogno,G., Alaggio,R. and Tartaglia,M.
  TITLE     RAF1 gene fusions are recurrent driver events in infantile
            fibrosarcoma-like mesenchymal tumors
  JOURNAL   J Pathol 263 (2), 166-177 (2024)
   PUBMED   38629245
  REMARK    GeneRIF: RAF1 gene fusions are recurrent driver events in infantile
            fibrosarcoma-like mesenchymal tumors.
REFERENCE   3  (bases 1 to 3191)
  AUTHORS   Hong,H., Yu,L., Cong,W., Kang,K., Gao,Y., Guan,Q., Meng,X.,
            Zhang,H. and Zhou,Z.
  TITLE     Cross-Talking Pathways of Rapidly Accelerated Fibrosarcoma-1
            (RAF-1) in Alzheimer's Disease
  JOURNAL   Mol Neurobiol 61 (5), 2798-2807 (2024)
   PUBMED   37940778
  REMARK    GeneRIF: Cross-Talking Pathways of Rapidly Accelerated
            Fibrosarcoma-1 (RAF-1) in Alzheimer's Disease.
REFERENCE   4  (bases 1 to 3191)
  AUTHORS   Kirkbride,J.A., Nilsson,G.Y., Kim,J.I., Takeya,K., Tanaka,Y.,
            Tokumitsu,H., Suizu,F. and Eto,M.
  TITLE     PHI-1, an Endogenous Inhibitor Protein for Protein Phosphatase-1
            and a Pan-Cancer Marker, Regulates Raf-1 Proteostasis
  JOURNAL   Biomolecules 13 (12), 1741 (2023)
   PUBMED   38136612
  REMARK    GeneRIF: PHI-1, an Endogenous Inhibitor Protein for Protein
            Phosphatase-1 and a Pan-Cancer Marker, Regulates Raf-1
            Proteostasis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3191)
  AUTHORS   Gelb,B.D. and Tartaglia,M.
  TITLE     Noonan Syndrome with Multiple Lentigines
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301557
REFERENCE   6  (bases 1 to 3191)
  AUTHORS   Roberts,A.E.
  TITLE     Noonan Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301303
REFERENCE   7  (bases 1 to 3191)
  AUTHORS   Maslinski,W., Remillard,B., Tsudo,M. and Strom,T.B.
  TITLE     Interleukin-2 (IL-2) induces tyrosine kinase-dependent
            translocation of active raf-1 from the IL-2 receptor into the
            cytosol
  JOURNAL   J Biol Chem 267 (22), 15281-15284 (1992)
   PUBMED   1639773
REFERENCE   8  (bases 1 to 3191)
  AUTHORS   Tory,K., Latif,F., Modi,W., Schmidt,L., Wei,M.H., Li,H., Cobler,P.,
            Orcutt,M.L., Delisio,J., Geil,L. et al.
  TITLE     A genetic linkage map of 96 loci on the short arm of human
            chromosome 3
  JOURNAL   Genomics 13 (2), 275-286 (1992)
   PUBMED   1612588
REFERENCE   9  (bases 1 to 3191)
  AUTHORS   Galland,F., Stefanova,M., Lafage,M. and Birnbaum,D.
  TITLE     Localization of the 5' end of the MCF2 oncogene to human chromosome
            15q15----q23
  JOURNAL   Cytogenet Cell Genet 60 (2), 114-116 (1992)
   PUBMED   1611909
REFERENCE   10 (bases 1 to 3191)
  AUTHORS   Li,P., Wood,K., Mamon,H., Haser,W. and Roberts,T.
  TITLE     Raf-1: a kinase currently without a cause but not lacking in
            effects
  JOURNAL   Cell 64 (3), 479-482 (1991)
   PUBMED   1846778
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC342671.1, BC018119.2 and
            BU683389.1.
            
            On May 21, 2020 this sequence version replaced NM_002880.3.
            
            Summary: This gene is the cellular homolog of viral raf gene
            (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K),
            which functions downstream of the Ras family of membrane associated
            GTPases to which it binds directly. Once activated, the cellular
            RAF1 protein can phosphorylate to activate the dual specificity
            protein kinases MEK1 and MEK2, which in turn phosphorylate to
            activate the serine/threonine specific protein kinases, ERK1 and
            ERK2. Activated ERKs are pleiotropic effectors of cell physiology
            and play an important role in the control of gene expression
            involved in the cell division cycle, apoptosis, cell
            differentiation and cell migration. Mutations in this gene are
            associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided
            by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (2), as well as variant 3, encodes
            isoform b.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC018119.2, X03484.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000251849.9/ ENSP00000251849.4
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-512               DC342671.1         85-596
            513-3184            BC018119.2         511-3182
            3185-3191           BU683389.1         17-23               c
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p25.2"
     gene            1..3191
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /note="Raf-1 proto-oncogene, serine/threonine kinase"
                     /db_xref="GeneID:5894"
                     /db_xref="HGNC:HGNC:9829"
                     /db_xref="MIM:164760"
     exon            1..305
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            306..538
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             332..2278
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /EC_number="2.7.11.1"
                     /note="isoform b is encoded by transcript variant 2; raf
                     proto-oncogene serine/threonine protein kinase; Oncogene
                     RAF1; RAF proto-oncogene serine/threonine-protein kinase;
                     proto-oncogene c-RAF; v-raf-1 murine leukemia viral
                     oncogene homolog 1; C-Raf proto-oncogene, serine/threonine
                     kinase; v-raf-1 murine leukemia viral oncogene-like
                     protein 1"
                     /codon_start=1
                     /product="RAF proto-oncogene serine/threonine-protein
                     kinase isoform b"
                     /protein_id="NP_002871.1"
                     /db_xref="CCDS:CCDS2612.1"
                     /db_xref="GeneID:5894"
                     /db_xref="HGNC:HGNC:9829"
                     /db_xref="MIM:164760"
                     /translation="MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASD
                     DGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLL
                     HEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLL
                     NGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMR
                     ESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRM
                     IEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRG
                     QRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRN
                     EVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIAR
                     QTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGS
                     VLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYA
                     SPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPS
                     LHRAAHTEDINACTLTTSPRLPVF"
     exon            539..651
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            652..754
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            755..912
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1011
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1165
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1193
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1321
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1322..1439
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1440..1524
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1525..1701
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1702..1748
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1749..1867
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1868..1999
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2000..2134
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2135..3191
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3165..3170
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /note="hexamer: AATAAA"
     regulatory      3175..3180
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /note="hexamer: ATTAAA"
     polyA_site      3184
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /note="major polyA site"
     polyA_site      3191
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
ORIGIN      
        1 aatcgcgggc gcttgggccg ccatcttaga tggcgggagt aagaggaaaa cgattgtgag
       61 gcgggaacgg ctttctgctg ccttttttgg gccccgaaaa gggtcagctg gccgggcttt
      121 ggggcgcgtg ccctgaggcg cggagcgcgt ttgctacgat gcgggggctg ctcggggctc
      181 cgtcccctgg gctggggacg cgccgaatgt gaccgcctcc cgctccctca cccgccgcgg
      241 ggaggaggag cgggcgagaa gctgccgccg aacgacagga cgttggggcg gcctggctcc
      301 ctcaggttta agaattgttt aagctgcatc aatggagcac atacagggag cttggaagac
      361 gatcagcaat ggttttggat tcaaagatgc cgtgtttgat ggctccagct gcatctctcc
      421 tacaatagtt cagcagtttg gctatcagcg ccgggcatca gatgatggca aactcacaga
      481 tccttctaag acaagcaaca ctatccgtgt tttcttgccg aacaagcaaa gaacagtggt
      541 caatgtgcga aatggaatga gcttgcatga ctgccttatg aaagcactca aggtgagggg
      601 cctgcaacca gagtgctgtg cagtgttcag acttctccac gaacacaaag gtaaaaaagc
      661 acgcttagat tggaatactg atgctgcgtc tttgattgga gaagaacttc aagtagattt
      721 cctggatcat gttcccctca caacacacaa ctttgctcgg aagacgttcc tgaagcttgc
      781 cttctgtgac atctgtcaga aattcctgct caatggattt cgatgtcaga cttgtggcta
      841 caaatttcat gagcactgta gcaccaaagt acctactatg tgtgtggact ggagtaacat
      901 cagacaactc ttattgtttc caaattccac tattggtgat agtggagtcc cagcactacc
      961 ttctttgact atgcgtcgta tgcgagagtc tgtttccagg atgcctgtta gttctcagca
     1021 cagatattct acacctcacg ccttcacctt taacacctcc agtccctcat ctgaaggttc
     1081 cctctcccag aggcagaggt cgacatccac acctaatgtc cacatggtca gcaccaccct
     1141 gcctgtggac agcaggatga ttgaggatgc aattcgaagt cacagcgaat cagcctcacc
     1201 ttcagccctg tccagtagcc ccaacaatct gagcccaaca ggctggtcac agccgaaaac
     1261 ccccgtgcca gcacaaagag agcgggcacc agtatctggg acccaggaga aaaacaaaat
     1321 taggcctcgt ggacagagag attcaagcta ttattgggaa atagaagcca gtgaagtgat
     1381 gctgtccact cggattgggt caggctcttt tggaactgtt tataagggta aatggcacgg
     1441 agatgttgca gtaaagatcc taaaggttgt cgacccaacc ccagagcaat tccaggcctt
     1501 caggaatgag gtggctgttc tgcgcaaaac acggcatgtg aacattctgc ttttcatggg
     1561 gtacatgaca aaggacaacc tggcaattgt gacccagtgg tgcgagggca gcagcctcta
     1621 caaacacctg catgtccagg agaccaagtt tcagatgttc cagctaattg acattgcccg
     1681 gcagacggct cagggaatgg actatttgca tgcaaagaac atcatccata gagacatgaa
     1741 atccaacaat atatttctcc atgaaggctt aacagtgaaa attggagatt ttggtttggc
     1801 aacagtaaag tcacgctgga gtggttctca gcaggttgaa caacctactg gctctgtcct
     1861 ctggatggcc ccagaggtga tccgaatgca ggataacaac ccattcagtt tccagtcgga
     1921 tgtctactcc tatggcatcg tattgtatga actgatgacg ggggagcttc cttattctca
     1981 catcaacaac cgagatcaga tcatcttcat ggtgggccga ggatatgcct ccccagatct
     2041 tagtaagcta tataagaact gccccaaagc aatgaagagg ctggtagctg actgtgtgaa
     2101 gaaagtaaag gaagagaggc ctctttttcc ccagatcctg tcttccattg agctgctcca
     2161 acactctcta ccgaagatca accggagcgc ttccgagcca tccttgcatc gggcagccca
     2221 cactgaggat atcaatgctt gcacgctgac cacgtccccg aggctgcctg tcttctagtt
     2281 gactttgcac ctgtcttcag gctgccaggg gaggaggaga agccagcagg caccactttt
     2341 ctgctccctt tctccagagg cagaacacat gttttcagag aagctgctgc taaggacctt
     2401 ctagactgct cacagggcct taacttcatg ttgccttctt ttctatccct ttgggccctg
     2461 ggagaaggaa gccatttgca gtgctggtgt gtcctgctcc ctccccacat tccccatgct
     2521 caaggcccag ccttctgtag atgcgcaagt ggatgttgat ggtagtacaa aaagcagggg
     2581 cccagcccca gctgttggct acatgagtat ttagaggaag taaggtagca ggcagtccag
     2641 ccctgatgtg gagacacatg ggattttgga aatcagcttc tggaggaatg catgtcacag
     2701 gcgggacttt cttcagagag tggtgcagcg ccagacattt tgcacataag gcaccaaaca
     2761 gcccaggact gccgagactc tggccgcccg aaggagcctg ctttggtact atggaacttt
     2821 tcttagggga cacgtcctcc tttcacagct tctaaggtgt ccagtgcatt gggatggttt
     2881 tccaggcaag gcactcggcc aatccgcatc tcagccctct cagggagcag tcttccatca
     2941 tgctgaattt tgtcttccag gagctgcccc tatggggcgg ggccgcaggg ccagccttgt
     3001 ttctctaaca aacaaacaaa caaacagcct tgtttctcta gtcacatcat gtgtatacaa
     3061 ggaagccagg aatacaggtt ttcttgatga tttgggtttt aattttgttt ttattgcacc
     3121 tgacaaaata cagttatctg atggtccctc aattatgtta ttttaataaa ataaattaaa
     3181 tttaggtgta a
//
