LOCUS       NM_001354693            3092 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens Raf-1 proto-oncogene, serine/threonine kinase (RAF1),
            transcript variant 6, mRNA.
ACCESSION   NM_001354693 XM_005265357
VERSION     NM_001354693.3
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Khoroshkin,M., Buyan,A., Dodel,M., Navickas,A., Yu,J., Trejo,F.,
            Doty,A., Baratam,R., Zhou,S., Lee,S.B., Joshi,T., Garcia,K.,
            Choi,B., Miglani,S., Subramanyam,V., Modi,H., Carpenter,C.,
            Markett,D., Corces,M.R., Mardakheh,F.K., Kulakovskiy,I.V. and
            Goodarzi,H.
  TITLE     Systematic identification of post-transcriptional regulatory
            modules
  JOURNAL   Nat Commun 15 (1), 7872 (2024)
   PUBMED   39251607
  REMARK    Erratum:[Nat Commun. 2024 Oct 28;15(1):9277. doi:
            10.1038/s41467-024-52903-4.. PMID: 39468017]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Motta,M., Barresi,S., Pizzi,S., Bifano,D., Lopez Marti,J.,
            Garrido-Pontnou,M., Flex,E., Bruselles,A., Giovannoni,I.,
            Rotundo,G., Fragale,A., Tirelli,V., Vallese,S., Ciolfi,A.,
            Bisogno,G., Alaggio,R. and Tartaglia,M.
  TITLE     RAF1 gene fusions are recurrent driver events in infantile
            fibrosarcoma-like mesenchymal tumors
  JOURNAL   J Pathol 263 (2), 166-177 (2024)
   PUBMED   38629245
  REMARK    GeneRIF: RAF1 gene fusions are recurrent driver events in infantile
            fibrosarcoma-like mesenchymal tumors.
REFERENCE   3  (bases 1 to 3092)
  AUTHORS   Hong,H., Yu,L., Cong,W., Kang,K., Gao,Y., Guan,Q., Meng,X.,
            Zhang,H. and Zhou,Z.
  TITLE     Cross-Talking Pathways of Rapidly Accelerated Fibrosarcoma-1
            (RAF-1) in Alzheimer's Disease
  JOURNAL   Mol Neurobiol 61 (5), 2798-2807 (2024)
   PUBMED   37940778
  REMARK    GeneRIF: Cross-Talking Pathways of Rapidly Accelerated
            Fibrosarcoma-1 (RAF-1) in Alzheimer's Disease.
REFERENCE   4  (bases 1 to 3092)
  AUTHORS   Kirkbride,J.A., Nilsson,G.Y., Kim,J.I., Takeya,K., Tanaka,Y.,
            Tokumitsu,H., Suizu,F. and Eto,M.
  TITLE     PHI-1, an Endogenous Inhibitor Protein for Protein Phosphatase-1
            and a Pan-Cancer Marker, Regulates Raf-1 Proteostasis
  JOURNAL   Biomolecules 13 (12), 1741 (2023)
   PUBMED   38136612
  REMARK    GeneRIF: PHI-1, an Endogenous Inhibitor Protein for Protein
            Phosphatase-1 and a Pan-Cancer Marker, Regulates Raf-1
            Proteostasis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3092)
  AUTHORS   Gelb,B.D. and Tartaglia,M.
  TITLE     Noonan Syndrome with Multiple Lentigines
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301557
REFERENCE   6  (bases 1 to 3092)
  AUTHORS   Roberts,A.E.
  TITLE     Noonan Syndrome
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301303
REFERENCE   7  (bases 1 to 3092)
  AUTHORS   Maslinski,W., Remillard,B., Tsudo,M. and Strom,T.B.
  TITLE     Interleukin-2 (IL-2) induces tyrosine kinase-dependent
            translocation of active raf-1 from the IL-2 receptor into the
            cytosol
  JOURNAL   J Biol Chem 267 (22), 15281-15284 (1992)
   PUBMED   1639773
REFERENCE   8  (bases 1 to 3092)
  AUTHORS   Tory,K., Latif,F., Modi,W., Schmidt,L., Wei,M.H., Li,H., Cobler,P.,
            Orcutt,M.L., Delisio,J., Geil,L. et al.
  TITLE     A genetic linkage map of 96 loci on the short arm of human
            chromosome 3
  JOURNAL   Genomics 13 (2), 275-286 (1992)
   PUBMED   1612588
REFERENCE   9  (bases 1 to 3092)
  AUTHORS   Galland,F., Stefanova,M., Lafage,M. and Birnbaum,D.
  TITLE     Localization of the 5' end of the MCF2 oncogene to human chromosome
            15q15----q23
  JOURNAL   Cytogenet Cell Genet 60 (2), 114-116 (1992)
   PUBMED   1611909
REFERENCE   10 (bases 1 to 3092)
  AUTHORS   Li,P., Wood,K., Mamon,H., Haser,W. and Roberts,T.
  TITLE     Raf-1: a kinase currently without a cause but not lacking in
            effects
  JOURNAL   Cell 64 (3), 479-482 (1991)
   PUBMED   1846778
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC026170.5 and AC018500.4.
            
            On May 21, 2020 this sequence version replaced NM_001354693.2.
            
            Summary: This gene is the cellular homolog of viral raf gene
            (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K),
            which functions downstream of the Ras family of membrane associated
            GTPases to which it binds directly. Once activated, the cellular
            RAF1 protein can phosphorylate to activate the dual specificity
            protein kinases MEK1 and MEK2, which in turn phosphorylate to
            activate the serine/threonine specific protein kinases, ERK1 and
            ERK2. Activated ERKs are pleiotropic effectors of cell physiology
            and play an important role in the control of gene expression
            involved in the cell division cycle, apoptosis, cell
            differentiation and cell migration. Mutations in this gene are
            associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided
            by RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR11853563.22112.1,
                                           SRR11853564.18787.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-305               AC026170.5         89534-89838         c
            306-538             AC026170.5         44236-44468         c
            539-651             AC026170.5         37671-37783         c
            652-754             AC026170.5         34954-35056         c
            755-912             AC018500.4         150529-150686       c
            913-1066            AC018500.4         145899-146052       c
            1067-1094           AC018500.4         142151-142178       c
            1095-1222           AC018500.4         141915-142042       c
            1223-1340           AC018500.4         141454-141571       c
            1341-1425           AC018500.4         133471-133555       c
            1426-1602           AC018500.4         132561-132737       c
            1603-1649           AC018500.4         129354-129400       c
            1650-1768           AC018500.4         127444-127562       c
            1769-1900           AC018500.4         126885-127016       c
            1901-2035           AC018500.4         126610-126744       c
            2036-3092           AC018500.4         125364-126420       c
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p25.2"
     gene            1..3092
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /note="Raf-1 proto-oncogene, serine/threonine kinase"
                     /db_xref="GeneID:5894"
                     /db_xref="HGNC:HGNC:9829"
                     /db_xref="MIM:164760"
     exon            1..305
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            306..538
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             332..2179
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /EC_number="2.7.11.1"
                     /note="isoform d is encoded by transcript variant 6; raf
                     proto-oncogene serine/threonine protein kinase; Oncogene
                     RAF1; RAF proto-oncogene serine/threonine-protein kinase;
                     proto-oncogene c-RAF; v-raf-1 murine leukemia viral
                     oncogene homolog 1; C-Raf proto-oncogene, serine/threonine
                     kinase; v-raf-1 murine leukemia viral oncogene-like
                     protein 1"
                     /codon_start=1
                     /product="RAF proto-oncogene serine/threonine-protein
                     kinase isoform d"
                     /protein_id="NP_001341622.1"
                     /db_xref="CCDS:CCDS93217.1"
                     /db_xref="GeneID:5894"
                     /db_xref="HGNC:HGNC:9829"
                     /db_xref="MIM:164760"
                     /translation="MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASD
                     DGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLL
                     HEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLL
                     NGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLFSQHRYSTPHAFTFNTSSPSSEGSL
                     SQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPK
                     TPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGK
                     WHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCE
                     GSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVK
                     IGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYEL
                     MTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLF
                     PQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF"
     exon            539..651
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            652..754
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            755..912
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1066
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1094
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1095..1222
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1340
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1425
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1602
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1603..1649
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1768
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1769..1900
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1901..2035
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2036..3092
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3066..3071
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /note="hexamer: AATAAA"
     regulatory      3076..3081
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /note="hexamer: ATTAAA"
     polyA_site      3085
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
                     /note="major polyA site"
     polyA_site      3092
                     /gene="RAF1"
                     /gene_synonym="c-Raf; CMD1NN; CRAF; NS5; Raf-1"
ORIGIN      
        1 aatcgcgggc gcttgggccg ccatcttaga tggcgggagt aagaggaaaa cgattgtgag
       61 gcgggaacgg ctttctgctg ccttttttgg gccccgaaaa gggtcagctg gccgggcttt
      121 ggggcgcgtg ccctgaggcg cggagcgcgt ttgctacgat gcgggggctg ctcggggctc
      181 cgtcccctgg gctggggacg cgccgaatgt gaccgcctcc cgctccctca cccgccgcgg
      241 ggaggaggag cgggcgagaa gctgccgccg aacgacagga cgttggggcg gcctggctcc
      301 ctcaggttta agaattgttt aagctgcatc aatggagcac atacagggag cttggaagac
      361 gatcagcaat ggttttggat tcaaagatgc cgtgtttgat ggctccagct gcatctctcc
      421 tacaatagtt cagcagtttg gctatcagcg ccgggcatca gatgatggca aactcacaga
      481 tccttctaag acaagcaaca ctatccgtgt tttcttgccg aacaagcaaa gaacagtggt
      541 caatgtgcga aatggaatga gcttgcatga ctgccttatg aaagcactca aggtgagggg
      601 cctgcaacca gagtgctgtg cagtgttcag acttctccac gaacacaaag gtaaaaaagc
      661 acgcttagat tggaatactg atgctgcgtc tttgattgga gaagaacttc aagtagattt
      721 cctggatcat gttcccctca caacacacaa ctttgctcgg aagacgttcc tgaagcttgc
      781 cttctgtgac atctgtcaga aattcctgct caatggattt cgatgtcaga cttgtggcta
      841 caaatttcat gagcactgta gcaccaaagt acctactatg tgtgtggact ggagtaacat
      901 cagacaactc ttttctcagc acagatattc tacacctcac gccttcacct ttaacacctc
      961 cagtccctca tctgaaggtt ccctctccca gaggcagagg tcgacatcca cacctaatgt
     1021 ccacatggtc agcaccaccc tgcctgtgga cagcaggatg attgaggatg caattcgaag
     1081 tcacagcgaa tcagcctcac cttcagccct gtccagtagc cccaacaatc tgagcccaac
     1141 aggctggtca cagccgaaaa cccccgtgcc agcacaaaga gagcgggcac cagtatctgg
     1201 gacccaggag aaaaacaaaa ttaggcctcg tggacagaga gattcaagct attattggga
     1261 aatagaagcc agtgaagtga tgctgtccac tcggattggg tcaggctctt ttggaactgt
     1321 ttataagggt aaatggcacg gagatgttgc agtaaagatc ctaaaggttg tcgacccaac
     1381 cccagagcaa ttccaggcct tcaggaatga ggtggctgtt ctgcgcaaaa cacggcatgt
     1441 gaacattctg cttttcatgg ggtacatgac aaaggacaac ctggcaattg tgacccagtg
     1501 gtgcgagggc agcagcctct acaaacacct gcatgtccag gagaccaagt ttcagatgtt
     1561 ccagctaatt gacattgccc ggcagacggc tcagggaatg gactatttgc atgcaaagaa
     1621 catcatccat agagacatga aatccaacaa tatatttctc catgaaggct taacagtgaa
     1681 aattggagat tttggtttgg caacagtaaa gtcacgctgg agtggttctc agcaggttga
     1741 acaacctact ggctctgtcc tctggatggc cccagaggtg atccgaatgc aggataacaa
     1801 cccattcagt ttccagtcgg atgtctactc ctatggcatc gtattgtatg aactgatgac
     1861 gggggagctt ccttattctc acatcaacaa ccgagatcag atcatcttca tggtgggccg
     1921 aggatatgcc tccccagatc ttagtaagct atataagaac tgccccaaag caatgaagag
     1981 gctggtagct gactgtgtga agaaagtaaa ggaagagagg cctctttttc cccagatcct
     2041 gtcttccatt gagctgctcc aacactctct accgaagatc aaccggagcg cttccgagcc
     2101 atccttgcat cgggcagccc acactgagga tatcaatgct tgcacgctga ccacgtcccc
     2161 gaggctgcct gtcttctagt tgactttgca cctgtcttca ggctgccagg ggaggaggag
     2221 aagccagcag gcaccacttt tctgctccct ttctccagag gcagaacaca tgttttcaga
     2281 gaagctgctg ctaaggacct tctagactgc tcacagggcc ttaacttcat gttgccttct
     2341 tttctatccc tttgggccct gggagaagga agccatttgc agtgctggtg tgtcctgctc
     2401 cctccccaca ttccccatgc tcaaggccca gccttctgta gatgcgcaag tggatgttga
     2461 tggtagtaca aaaagcaggg gcccagcccc agctgttggc tacatgagta tttagaggaa
     2521 gtaaggtagc aggcagtcca gccctgatgt ggagacacat gggattttgg aaatcagctt
     2581 ctggaggaat gcatgtcaca ggcgggactt tcttcagaga gtggtgcagc gccagacatt
     2641 ttgcacataa ggcaccaaac agcccaggac tgccgagact ctggccgccc gaaggagcct
     2701 gctttggtac tatggaactt ttcttagggg acacgtcctc ctttcacagc ttctaaggtg
     2761 tccagtgcat tgggatggtt ttccaggcaa ggcactcggc caatccgcat ctcagccctc
     2821 tcagggagca gtcttccatc atgctgaatt ttgtcttcca ggagctgccc ctatggggcg
     2881 gggccgcagg gccagccttg tttctctaac aaacaaacaa acaaacagcc ttgtttctct
     2941 agtcacatca tgtgtataca aggaagccag gaatacaggt tttcttgatg atttgggttt
     3001 taattttgtt tttattgcac ctgacaaaat acagttatct gatggtccct caattatgtt
     3061 attttaataa aataaattaa atttaggtgt aa
//
