LOCUS       NM_001286535            4164 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens RAD9 checkpoint clamp component B (RAD9B), transcript
            variant 6, mRNA.
ACCESSION   NM_001286535
VERSION     NM_001286535.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Barroso-Chinea,P., Salas-Hernandez,J., Cruz-Muros,I.,
            Lopez-Fernandez,J., Freire,R. and Afonso-Oramas,D.
  TITLE     Expression of RAD9B in the mesostriatal system of rats and humans:
            Overexpression in a 6-OHDA rat model of Parkinson's disease
  JOURNAL   Ann Anat 250, 152135 (2023)
   PUBMED   37460044
  REMARK    GeneRIF: Expression of RAD9B in the mesostriatal system of rats and
            humans: Overexpression in a 6-OHDA rat model of Parkinson's
            disease.
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Cao,X., Tian,T., Steele,J.W., Cabrera,R.M., Aguiar-Pulido,V.,
            Wadhwa,S., Bhavani,N., Bi,P., Gargurevich,N.H., Hoffman,E.N.,
            Cai,C.Q., Marini,N.J., Yang,W., Shaw,G.M., Ross,M.E., Finnell,R.H.
            and Lei,Y.
  TITLE     Loss of RAD9B impairs early neural development and contributes to
            the risk for human spina bifida
  JOURNAL   Hum Mutat 41 (4), 786-799 (2020)
   PUBMED   31898828
  REMARK    GeneRIF: Loss of RAD9B impairs early neural development and
            contributes to the risk for human spina bifida.
            Erratum:[Hum Mutat. 2021 Apr;42(4):487. doi: 10.1002/humu.24175..
            PMID: 33856736]
REFERENCE   3  (bases 1 to 4164)
  AUTHORS   Lyndaker,A.M., Vasileva,A., Wolgemuth,D.J., Weiss,R.S. and
            Lieberman,H.B.
  TITLE     Clamping down on mammalian meiosis
  JOURNAL   Cell Cycle 12 (19), 3135-3145 (2013)
   PUBMED   24013428
REFERENCE   4  (bases 1 to 4164)
  AUTHORS   Perez-Castro,A.J. and Freire,R.
  TITLE     Rad9B responds to nucleolar stress through ATR and JNK signalling,
            and delays the G1-S transition
  JOURNAL   J Cell Sci 125 (Pt 5), 1152-1164 (2012)
   PUBMED   22399810
  REMARK    GeneRIF: Rad9B responds to nucleolar stress after the activation of
            JNK-dependent stress signalling and ATR-dependent DNA damage
            response pathways, and actively accumulates within nucleoli, where
            it does not cause a transcription block.
REFERENCE   5  (bases 1 to 4164)
  AUTHORS   Lieberman,H.B.
  TITLE     Rad9, an evolutionarily conserved gene with multiple functions for
            preserving genomic integrity
  JOURNAL   J Cell Biochem 97 (4), 690-697 (2006)
   PUBMED   16365875
  REMARK    GeneRIF: The encoded mammalian proteins participate in promoting
            resistance to DNA damage, cell cycle checkpoint control, DNA
            repair, and apoptosis.
            Review article
REFERENCE   6  (bases 1 to 4164)
  AUTHORS   Dufault,V.M., Oestreich,A.J., Vroman,B.T. and Karnitz,L.M.
  TITLE     Identification and characterization of RAD9B, a paralog of the RAD9
            checkpoint gene
  JOURNAL   Genomics 82 (6), 644-651 (2003)
   PUBMED   14611806
REFERENCE   7  (bases 1 to 4164)
  AUTHORS   Hopkins,K.M., Wang,X., Berlin,A., Hang,H., Thaker,H.M. and
            Lieberman,H.B.
  TITLE     Expression of mammalian paralogues of HRAD9 and Mrad9 checkpoint
            control genes in normal and cancerous testicular tissue
  JOURNAL   Cancer Res 63 (17), 5291-5298 (2003)
   PUBMED   14500360
REFERENCE   8  (bases 1 to 4164)
  AUTHORS   Zou,L. and Elledge,S.J.
  TITLE     Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes
  JOURNAL   Science 300 (5625), 1542-1548 (2003)
   PUBMED   12791985
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC002350.1.
            
            On Jan 10, 2019 this sequence version replaced NM_001286535.1.
            
            Transcript Variant: This variant (6) differs in the exon structure
            in the 3' coding region, compared to variant 1. The encoded isoform
            (d) has a distinct C-terminus and is shorter than isoform a.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY297459.1, SRR1803612.77992.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000409300.6/ ENSP00000386434.1
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-53                AC002350.1         113422-113474       c
            54-124              AC002350.1         111929-111999       c
            125-280             AC002350.1         110033-110188       c
            281-395             AC002350.1         109112-109226       c
            396-495             AC002350.1         102927-103026       c
            496-602             AC002350.1         100649-100755       c
            603-709             AC002350.1         97023-97129         c
            710-774             AC002350.1         96867-96931         c
            775-897             AC002350.1         95889-96011         c
            898-1132            AC002350.1         93394-93628         c
            1133-4164           AC002350.1         82249-85280         c
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.11"
     gene            1..4164
                     /gene="RAD9B"
                     /note="RAD9 checkpoint clamp component B"
                     /db_xref="GeneID:144715"
                     /db_xref="HGNC:HGNC:21700"
                     /db_xref="MIM:608368"
     exon            1..53
                     /gene="RAD9B"
                     /inference="alignment:Splign:2.1.0"
     CDS             8..1261
                     /gene="RAD9B"
                     /EC_number="3.1.11.2"
                     /note="isoform d is encoded by transcript variant 6; DNA
                     repair exonuclease rad9 homolog B; cell cycle checkpoint
                     control protein RAD9B; RAD9 homolog B"
                     /codon_start=1
                     /product="cell cycle checkpoint control protein RAD9B
                     isoform d"
                     /protein_id="NP_001273464.1"
                     /db_xref="CCDS:CCDS73526.1"
                     /db_xref="GeneID:144715"
                     /db_xref="HGNC:HGNC:21700"
                     /db_xref="MIM:608368"
                     /translation="MAAMLKCVMSGSQVKVFGKAVQALSRISDEFWLDPSKKGLALRC
                     VNSSRSAYGCVLFSPVFFQHYQWSALVKMSENELDTTLHLKCKLGMKSILPIFRCLNS
                     LERNIEKCRIFTRSDKCKVVIQFFYRHGIKRTHNICFQESQPLQVIFDKNVCTNTLMI
                     QPRLLADAIVLFTSSQEEVTLAVTPLNFCLKSSNEESMDLSNAVHSEMFVGSDEFDFF
                     QIGMDTEITFCFKELKGILTFSEATHAPISIYFDFPGKPLALSIDDMLVEANFILATL
                     ADEQSRASSPQSLCLSQKRKRSDLIEKKAGKNVTGQALECISKKAAPRRLYPKETLTN
                     ISALENCGSPAMKRVDGDVSEVSESSVSNTEEVPGSLCLRKFSCMFFGAVSSDQQEHF
                     NHPFDSLARASDSEEDMNNGSFSIF"
     exon            54..124
                     /gene="RAD9B"
                     /inference="alignment:Splign:2.1.0"
     exon            125..280
                     /gene="RAD9B"
                     /inference="alignment:Splign:2.1.0"
     exon            281..395
                     /gene="RAD9B"
                     /inference="alignment:Splign:2.1.0"
     exon            396..495
                     /gene="RAD9B"
                     /inference="alignment:Splign:2.1.0"
     exon            496..602
                     /gene="RAD9B"
                     /inference="alignment:Splign:2.1.0"
     exon            603..709
                     /gene="RAD9B"
                     /inference="alignment:Splign:2.1.0"
     exon            710..774
                     /gene="RAD9B"
                     /inference="alignment:Splign:2.1.0"
     exon            775..897
                     /gene="RAD9B"
                     /inference="alignment:Splign:2.1.0"
     exon            898..1132
                     /gene="RAD9B"
                     /inference="alignment:Splign:2.1.0"
     exon            1133..4164
                     /gene="RAD9B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2671..2676
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD9B"
                     /note="hexamer: AATAAA"
     polyA_site      2694
                     /gene="RAD9B"
                     /note="major polyA site"
     regulatory      4146..4151
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD9B"
                     /note="hexamer: AATAAA"
     polyA_site      4164
                     /gene="RAD9B"
ORIGIN      
        1 agggcggatg gcagccatgc tgaagtgcgt gatgagcggc agtcaggtga aagtatttgg
       61 gaaagcagtt caagctctat cacgaattag tgacgagttc tggctagacc catctaaaaa
      121 aggtcttgct ctaagatgtg tgaattcttc tcggtcagca tatggatgtg tcctgttctc
      181 tcctgtgttt tttcagcatt atcaatggtc agctttagtg aaaatgagtg aaaatgaact
      241 tgacacaaca ctgcatttaa aatgcaaatt gggaatgaag tcaattttgc ccatctttag
      301 atgtctgaat tcccttgaaa gaaatataga gaagtgcaga atattcacca gatctgataa
      361 atgcaaagta gttattcaat tcttctacag acatggtatt aaaagaactc ataatatatg
      421 ttttcaagaa agtcagcctt tgcaagttat ttttgacaag aatgtttgta ctaatacgct
      481 aatgattcaa ccaagattgc ttgctgatgc cattgttctt tttacatcaa gtcaagagga
      541 agttactctt gctgttactc cactgaattt ttgcctcaag agttctaatg aggaatcaat
      601 ggatttgagc aatgctgtac acagtgagat gtttgttggc tcagatgagt ttgacttctt
      661 tcaaattgga atggacactg agataacatt ttgtttcaaa gaattgaagg gaatactgac
      721 attttcagaa gctacacatg ctcctatatc catttatttt gatttccctg ggaaacctct
      781 ggctttgagt attgatgata tgttagtgga agctaacttt attttggcca cattagctga
      841 tgaacaaagt agagcatctt caccacagtc actgtgtctt tcacagaaac gaaaaaggtc
      901 agatctgatt gaaaaaaagg ctggcaaaaa tgtaactggc caggccctgg aatgtatttc
      961 aaaaaaagca gcaccaagaa ggctttatcc taaggagact ctcacaaaca tatctgcatt
     1021 ggaaaactgt ggcagccctg caatgaaaag agtggatgga gatgtcagtg aagtatcaga
     1081 aagcagtgtc agcaacacag aggaagtgcc agggtctctg tgtctcagaa agttttcttg
     1141 catgttcttt ggagcagttt cttctgacca gcaagaacac ttcaaccacc ctttcgacag
     1201 tctggcaaga gcaagtgaca gtgaagagga catgaataat ggcagtttct ctatattcta
     1261 atgcttaatg atggctgagc tgggccccag cccagtgact ggctcatttg cccctcaagc
     1321 acgagtttgc atgtttagtg tctaaaagag gttgtccagg acttcctttt aatggaggat
     1381 gggcttttaa accacatcat cttgtacaac aaccatatct agaaatagct gtttgtcaag
     1441 tgtatgtaac ttgctttaaa tccattatgc tacttgtgag gcagaagagt tttctgtgaa
     1501 ggaaaaaagc ccattagagt tcttcaattc aatgcacgtt caccctagag cttttaacat
     1561 ctttgctagt tttataaagg tatttaaact ttattcaaca gccatttaga gtgccatcaa
     1621 gatggcttga aatggaattt tgtgatttgt agtcaggtat cttttgtatt tgattgcaaa
     1681 catttggatt ttagttttct catgtaatac catggccttt tttgtgcatt gttttttata
     1741 ttttaagact ttaagtagaa taaaccctgg aaaaaagatc aagagtaaaa atatatagtc
     1801 actttcactt ggctttttta gacggagtct cactttgtca ctcaggctca agtgcagtgg
     1861 tgcaatctct gctcactgca acatctgcct cccaggtcca agcgattctc ctgcctcagc
     1921 ctcccgtgca gctgggattg caggtgcgtg ccaccatgcc tggctaattt ctggtatttt
     1981 gtagagacag ggtttcgcca tgttggccag ggtggtcttg aactcctggc ctcaagtgat
     2041 ctgcccacct cggcctccca aagtgctggg attacagact tgagccactg cgcccaacct
     2101 ggagtgtttt tacatattgt aaaattttat ttcctaacct caaattgttc tgattttcag
     2161 atgtgatttt ttattttgca gtgtgctgca ggaaagaatt taatggaagt gatgccaaat
     2221 atttctgtat tatctgacat agaacagtat cctccactgc caagacagcc tgagtttgga
     2281 gtggaataag gtggaagaca aatgtctctg ttctttggcc ctttaagagt tagcttttta
     2341 cctgcacaaa tggactaaaa aatctggcac aaaacattgt tatgtaatgt cttatgatgt
     2401 gtgcctctcc ctcccccaaa cctgtttaca gtcaattata acctgacaaa cgagactttt
     2461 gtaacatatt attgttacat ctttctgaaa ccttcaaacc gtaaggaagt gttaactggc
     2521 aagcagttgt actttagact ttgtgagaaa ttcataaagg tggctgagtg gatttgcatg
     2581 ctttagaact gtgaatagag ttctaactga aaccagaatt aatttggctc ttgtagctta
     2641 gtaatgagtc atagctaccc acaataacct aataaaaact caagttcatc ccaagatgtt
     2701 ccttagtgaa tgaaaatggg ccttaaccaa gctttttgtg gtaactggca atgcccacat
     2761 aatttcaact aagtcctttc atcacatggt aggggaagat atatgtatct tatgcttaca
     2821 ctgatgatta acactgaatt tctggtattt ctaacaaatg cagatgccta cacagggagc
     2881 ttttatacac aaaagtaact ctgtaggtct ctttgctgag gataacattt ctggttcatt
     2941 cattgaagta ggcagagggg tttaggtctt aaaggcatca tcatcagtaa tagcctggat
     3001 gcagcattgg actattatta tagttaatac tggcggggtg tagtggctca tgcctgtaat
     3061 cccagcactt tgggaggccc aggtgggcgg atcagttgag cccagcagtt caagactagc
     3121 ctgggcaaca tggtgagatc ccatctctac aaaaattagc tgggcacttt ggattttaga
     3181 atttggatac agagtgctaa gctgaaatac agtcatgtgc tgcataacaa cgttttggtc
     3241 aacgatggac tgcacagaca gtggtggtcc cataagataa tggagctgcc ctatacaggt
     3301 gtaccatttt tttgtctttc ataccatagt tttgctgaaa catttctatg tttagatata
     3361 cagatgctta ctgttgtgtt acaactgcct acagtattca gtacagtaac atgttataca
     3421 ggtttgtcgc ctaggagcaa caggctattc atatagccca gatgtgtagt aggctagacc
     3481 atctaggctt gtgtcagtac acaatgatga aattgctaag gatgcatctg tcagaacatc
     3541 tctgttaaac aacacgactg actgtacagc agattttttt tgtagcttcc ctctatacta
     3601 tacggaaggt gcaaagcctc agctgggaaa aagttctata atttacattt aattgtccgt
     3661 ccattttgat gaatttctga ggaagctaaa ctgatgtagt gaagcagctg gagccagtta
     3721 aagtagcagc atataatcag tgctagaatg actaggaggc ttggaaatga ggacctgata
     3781 gtgaccaaca aactcaatgt caagggctgg tgtctcattt agttactatt caaaccaaaa
     3841 gaagcacaca gattagtata aatactatat ttattaaata tctttacagt ttatttaaat
     3901 gtatttacag aactattcct gcataagtta taattcagac atccaaattc aggtaattgc
     3961 ctcttgggta ataagacaaa taatgatagt ctcaacagaa aaaaagcagc tcattagtat
     4021 acacacagat tctaatttgc ttcattattt agccatcttt gccacaaact acctgctaac
     4081 agttaaaatt ctgacatgga cacgttttag ttgagaagtt caattttgca aagaatttaa
     4141 ttttaaataa atagaatcca aata
//
