LOCUS       NM_004584               2086 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens RAD9 checkpoint clamp component A (RAD9A), transcript
            variant 1, mRNA.
ACCESSION   NM_004584
VERSION     NM_004584.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Yang,T., Liu,Y.L., Guo,H.L., Peng,X.F., Zhang,B., Wang,D.,
            Yao,H.F., Zhang,J.F., Wang,X.Y., Chen,P.C. and Xu,D.P.
  TITLE     Unveiling an anoikis-related risk model and the role of RAD9A in
            colon cancer
  JOURNAL   Int Immunopharmacol 140, 112874 (2024)
   PUBMED   39116498
  REMARK    GeneRIF: Unveiling an anoikis-related risk model and the role of
            RAD9A in colon cancer.
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Hara,K., Tatsukawa,K., Nagata,K., Iida,N., Hishiki,A., Ohashi,E.
            and Hashimoto,H.
  TITLE     Structural basis for intra- and intermolecular interactions on RAD9
            subunit of 9-1-1 checkpoint clamp implies functional 9-1-1
            regulation by RHINO
  JOURNAL   J Biol Chem 300 (3), 105751 (2024)
   PUBMED   38354779
  REMARK    GeneRIF: Structural basis for intra- and intermolecular
            interactions on RAD9 subunit of 9-1-1 checkpoint clamp implies
            functional 9-1-1 regulation by RHINO.
REFERENCE   3  (bases 1 to 2086)
  AUTHORS   Hara,K., Hishiki,A., Hoshino,T., Nagata,K., Iida,N., Sawada,Y.,
            Ohashi,E. and Hashimoto,H.
  TITLE     The 9-1-1 DNA clamp subunit RAD1 forms specific interactions with
            clamp loader RAD17, revealing functional implications for
            binding-protein RHINO
  JOURNAL   J Biol Chem 299 (4), 103061 (2023)
   PUBMED   36841485
REFERENCE   4  (bases 1 to 2086)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 2086)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   6  (bases 1 to 2086)
  AUTHORS   Bessho,T. and Sancar,A.
  TITLE     Human DNA damage checkpoint protein hRAD9 is a 3' to 5' exonuclease
  JOURNAL   J Biol Chem 275 (11), 7451-7454 (2000)
   PUBMED   10713044
REFERENCE   7  (bases 1 to 2086)
  AUTHORS   Komatsu,K., Miyashita,T., Hang,H., Hopkins,K.M., Zheng,W.,
            Cuddeback,S., Yamada,M., Lieberman,H.B. and Wang,H.G.
  TITLE     Human homologue of S. pombe Rad9 interacts with BCL-2/BCL-xL and
            promotes apoptosis
  JOURNAL   Nat Cell Biol 2 (1), 1-6 (2000)
   PUBMED   10620799
REFERENCE   8  (bases 1 to 2086)
  AUTHORS   St Onge,R.P., Udell,C.M., Casselman,R. and Davey,S.
  TITLE     The human G2 checkpoint control protein hRAD9 is a nuclear
            phosphoprotein that forms complexes with hRAD1 and hHUS1
  JOURNAL   Mol Biol Cell 10 (6), 1985-1995 (1999)
   PUBMED   10359610
REFERENCE   9  (bases 1 to 2086)
  AUTHORS   Volkmer,E. and Karnitz,L.M.
  TITLE     Human homologs of Schizosaccharomyces pombe rad1, hus1, and rad9
            form a DNA damage-responsive protein complex
  JOURNAL   J Biol Chem 274 (2), 567-570 (1999)
   PUBMED   9872989
REFERENCE   10 (bases 1 to 2086)
  AUTHORS   Lieberman,H.B., Hopkins,K.M., Nass,M., Demetrick,D. and Davey,S.
  TITLE     A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint
            control gene
  JOURNAL   Proc Natl Acad Sci U S A 93 (24), 13890-13895 (1996)
   PUBMED   8943031
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC014848.1, U53174.1 and
            AA708970.1.
            
            On Nov 23, 2018 this sequence version replaced NM_004584.2.
            
            Summary: This gene product is highly similar to Schizosaccharomyces
            pombe rad9, a cell cycle checkpoint protein required for cell cycle
            arrest and DNA damage repair. This protein possesses 3' to 5'
            exonuclease activity, which may contribute to its role in sensing
            and repairing DNA damage. It forms a checkpoint protein complex
            with RAD1 and HUS1. This complex is recruited by checkpoint protein
            RAD17 to the sites of DNA damage, which is thought to be important
            for triggering the checkpoint-signaling cascade. Alternatively
            spliced transcript variants encoding different isoforms have been
            found for this gene. [provided by RefSeq, Aug 2011].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript, and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U53174.1, BC014848.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000307980.7/ ENSP00000311360.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-447               BC014848.1         35-481
            448-2085            U53174.1           465-2102
            2086-2086           AA708970.1         9-9                 c
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q13.2"
     gene            1..2086
                     /gene="RAD9A"
                     /gene_synonym="RAD9"
                     /note="RAD9 checkpoint clamp component A"
                     /db_xref="GeneID:5883"
                     /db_xref="HGNC:HGNC:9827"
                     /db_xref="MIM:603761"
     exon            1..93
                     /gene="RAD9A"
                     /gene_synonym="RAD9"
                     /inference="alignment:Splign:2.1.0"
     CDS             60..1235
                     /gene="RAD9A"
                     /gene_synonym="RAD9"
                     /EC_number="3.1.11.2"
                     /note="isoform 1 is encoded by transcript variant 1; cell
                     cycle checkpoint control protein RAD9A; hRAD9; DNA repair
                     exonuclease rad9 homolog A; RAD9 homolog A"
                     /codon_start=1
                     /product="cell cycle checkpoint control protein RAD9A
                     isoform 1"
                     /protein_id="NP_004575.1"
                     /db_xref="CCDS:CCDS8159.1"
                     /db_xref="GeneID:5883"
                     /db_xref="HGNC:HGNC:9827"
                     /db_xref="MIM:603761"
                     /translation="MKCLVTGGNVKVLGKAVHSLSRIGDELYLEPLEDGLSLRTVNSS
                     RSAYACFLFAPLFFQQYQAATPGQDLLRCKILMKSFLSVFRSLAMLEKTVEKCCISLN
                     GRSSRLVVQLHCKFGVRKTHNLSFQDCESLQAVFDPASCPHMLRAPARVLGEAVLPFS
                     PALAEVTLGIGRGRRVILRSYHEEEADSTAKAMVTEMCLGEEDFQQLQAQEGVAITFC
                     LKEFRGLLSFAESANLNLSIHFDAPGRPAIFTIKDSLLDGHFVLATLSDTDSHSQDLG
                     SPERHQPVPQLQAHSTPHPDDFANDDIDSYMIAMETTIGNEGSRVLPSISLSPGPQPP
                     KSPGPHSEEEDEAEPSTVPGTPPPKKFRSLFFGSILAPVRSPQGPSPVLAEDSEGEG"
     exon            94..164
                     /gene="RAD9A"
                     /gene_synonym="RAD9"
                     /inference="alignment:Splign:2.1.0"
     exon            165..293
                     /gene="RAD9A"
                     /gene_synonym="RAD9"
                     /inference="alignment:Splign:2.1.0"
     exon            294..408
                     /gene="RAD9A"
                     /gene_synonym="RAD9"
                     /inference="alignment:Splign:2.1.0"
     exon            409..508
                     /gene="RAD9A"
                     /gene_synonym="RAD9"
                     /inference="alignment:Splign:2.1.0"
     exon            509..618
                     /gene="RAD9A"
                     /gene_synonym="RAD9"
                     /inference="alignment:Splign:2.1.0"
     exon            619..728
                     /gene="RAD9A"
                     /gene_synonym="RAD9"
                     /inference="alignment:Splign:2.1.0"
     exon            729..793
                     /gene="RAD9A"
                     /gene_synonym="RAD9"
                     /inference="alignment:Splign:2.1.0"
     exon            794..931
                     /gene="RAD9A"
                     /gene_synonym="RAD9"
                     /inference="alignment:Splign:2.1.0"
     exon            932..1139
                     /gene="RAD9A"
                     /gene_synonym="RAD9"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..2086
                     /gene="RAD9A"
                     /gene_synonym="RAD9"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1903
                     /gene="RAD9A"
                     /gene_synonym="RAD9"
     regulatory      2064..2069
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD9A"
                     /gene_synonym="RAD9"
                     /note="hexamer: ATTAAA"
     polyA_site      2086
                     /gene="RAD9A"
                     /gene_synonym="RAD9"
                     /note="major polyA site"
ORIGIN      
        1 ggacccggag gtcgcggaga gctgggcagt gttggccgct ggcggagcgc tggggcagca
       61 tgaagtgcct ggtcacgggc ggcaacgtga aggtgctcgg caaggccgtc cactccctgt
      121 cccgcatcgg ggacgagctc tacctggaac ccttggagga cgggctctcc ctccggacgg
      181 tgaactcctc ccgctctgcc tatgcctgct ttctctttgc cccgctcttc ttccagcaat
      241 accaggcagc cacccctggt caggacctgc tgcgctgtaa gatcctgatg aagtctttcc
      301 tgtctgtctt ccgctcactg gcgatgctgg agaagacggt ggaaaaatgc tgcatctccc
      361 tgaatggccg gagcagccgc ctggtggtcc agctgcattg caagttcggg gtgcggaaga
      421 ctcacaacct gtccttccag gactgtgagt ccctgcaggc cgtcttcgac ccagcctcgt
      481 gcccccacat gctccgcgcc ccagcacggg ttctggggga ggctgttctg cccttctctc
      541 ctgcactggc tgaagtgacg ctgggcattg gccgtggccg cagggtcatc ctgcgcagct
      601 accacgagga ggaggcagac agcactgcca aagccatggt gactgagatg tgccttggag
      661 aggaggattt ccagcagctg caggcccagg aaggggtggc catcactttc tgcctcaagg
      721 aattccgggg gctcctgagc tttgcagagt cagcaaactt gaatcttagc attcattttg
      781 atgctccagg caggcccgcc atcttcacca tcaaggactc tttgctggac ggccactttg
      841 tcttggccac actctcagac accgactcgc actcccagga cctgggctcc ccagagcgtc
      901 accagccagt gcctcagctc caggctcaca gcacacccca cccggacgac tttgccaatg
      961 acgacattga ctcttacatg atcgccatgg aaaccactat aggcaatgag ggctcgcggg
     1021 tgctgccctc catttccctt tcacctggcc cccagccccc caagagcccc ggtccccact
     1081 ccgaggagga agatgaggct gagcccagta cagtgcctgg gactccccca cccaagaagt
     1141 tccgctcact gttcttcggc tccatcctgg cccctgtacg ctccccccag ggccccagcc
     1201 ctgtgctggc ggaagacagt gagggtgaag gctgaaccaa gaacctgaag cctgtaccca
     1261 gaggccttgg actagacgaa gccccagcca gtggcagaac tgggtctctc agccctgggg
     1321 atcagaaagg tgggcttgct ggagctgagc tgtttcactg cctctcgcag gccccagctg
     1381 gctgtcactg taaagctgtc ccacagcggt cgggcctggg ccgttatctc cccacaaccc
     1441 ccagccaatc aggactttcc agacttggcc ctgaactact gacgttccta cctcttattt
     1501 ctcattgagc ctcaggctat actccagctg gccaaggctg gaaacctgtc tccctcaggc
     1561 tcaccttcct aaggaaaatg tcatagtagg tgctgctggc ccctggtgat ccagcttctc
     1621 tgccaatcat gacctgttcc ttcctgaagt cctgggcatg catctgggac ccccgtggag
     1681 ctgacaagtt ttccttgctt tcctgatact ctttggcgct gacttggaat tctaagagcc
     1741 ttggacccga gtgtgtggct agggttgccc tggctggggc ccggtgccga gactcccaag
     1801 cggctctgtg cagaagagct gccaggcagt gtcttagatg tgagacggag gccatggcga
     1861 gaatccagct ttgaccttta ttcaagagac cagatgggtt gccccaggat ccggctgcca
     1921 gccctgaggc caagcacggc tggagaccca cgacctggcc tgccgttgcc ctgagctgca
     1981 gcctcggccc caggatcctg ctcacagtca ccgcaggtgc aggcaggaag cagccctggg
     2041 ggactggacg ctgctattga ttcattaaaa aaagaaaaga aaaata
//
