LOCUS       NM_134424               3023 bp    mRNA    linear   PRI 11-FEB-2025
DEFINITION  Homo sapiens RAD52 homolog, DNA repair protein (RAD52), transcript
            variant 1, mRNA.
ACCESSION   NM_134424 NM_002879
VERSION     NM_134424.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Jalan,M., Sharma,A., Pei,X., Weinhold,N., Buechelmaier,E.S.,
            Zhu,Y., Ahmed-Seghir,S., Ratnakumar,A., Di Bona,M., McDermott,N.,
            Gomez-Aguilar,J., Anderson,K.S., Ng,C.K.Y., Selenica,P.,
            Bakhoum,S.F., Reis-Filho,J.S., Riaz,N. and Powell,S.N.
  TITLE     RAD52 resolves transcription-replication conflicts to mitigate
            R-loop induced genome instability
  JOURNAL   Nat Commun 15 (1), 7776 (2024)
   PUBMED   39237529
  REMARK    GeneRIF: RAD52 resolves transcription-replication conflicts to
            mitigate R-loop induced genome instability.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Struble,L.R., Lovelace,J.J. and Borgstahl,G.E.O.
  TITLE     A glimpse into the hidden world of the flexible C-terminal protein
            binding domains of human RAD52
  JOURNAL   J Struct Biol 216 (3), 108115 (2024)
   PUBMED   39117045
  REMARK    GeneRIF: A glimpse into the hidden world of the flexible C-terminal
            protein binding domains of human RAD52.
REFERENCE   3  (bases 1 to 3023)
  AUTHORS   Liang,C.C., Greenhough,L.A., Masino,L., Maslen,S., Bajrami,I.,
            Tuppi,M., Skehel,M., Taylor,I.A. and West,S.C.
  TITLE     Mechanism of single-stranded DNA annealing by RAD52-RPA complex
  JOURNAL   Nature 629 (8012), 697-703 (2024)
   PUBMED   38658755
  REMARK    GeneRIF: Mechanism of single-stranded DNA annealing by RAD52-RPA
            complex.
REFERENCE   4  (bases 1 to 3023)
  AUTHORS   Audrey,A., Kok,Y.P., Yu,S., de Haan,L., van de Kooij,B., van den
            Tempel,N., Chen,M., de Boer,H.R., van der Vegt,B. and van
            Vugt,M.A.T.M.
  TITLE     RAD52-dependent mitotic DNA synthesis is required for genome
            stability in Cyclin E1-overexpressing cells
  JOURNAL   Cell Rep 43 (4), 114116 (2024)
   PUBMED   38625790
  REMARK    GeneRIF: RAD52-dependent mitotic DNA synthesis is required for
            genome stability in Cyclin E1-overexpressing cells.
REFERENCE   5  (bases 1 to 3023)
  AUTHORS   Muhammad,A.A., Basto,C., Peterlini,T., Guirouilh-Barbat,J.,
            Thomas,M., Veaute,X., Busso,D., Lopez,B., Mazon,G., Le Cam,E.,
            Masson,J.Y. and Dupaigne,P.
  TITLE     Human RAD52 stimulates the RAD51-mediated homology search
  JOURNAL   Life Sci Alliance 7 (3), e202201751 (2023)
   PUBMED   38081641
  REMARK    GeneRIF: Human RAD52 stimulates the RAD51-mediated homology search.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3023)
  AUTHORS   Shen,Z., Pardington-Purtymun,P.E., Comeaux,J.C., Moyzis,R.K. and
            Chen,D.J.
  TITLE     UBL1, a human ubiquitin-like protein associating with human
            RAD51/RAD52 proteins
  JOURNAL   Genomics 36 (2), 271-279 (1996)
   PUBMED   8812453
REFERENCE   7  (bases 1 to 3023)
  AUTHORS   Park,M.S., Ludwig,D.L., Stigger,E. and Lee,S.H.
  TITLE     Physical interaction between human RAD52 and RPA is required for
            homologous recombination in mammalian cells
  JOURNAL   J Biol Chem 271 (31), 18996-19000 (1996)
   PUBMED   8702565
REFERENCE   8  (bases 1 to 3023)
  AUTHORS   Park,M.S.
  TITLE     Expression of human RAD52 confers resistance to ionizing radiation
            in mammalian cells
  JOURNAL   J Biol Chem 270 (26), 15467-15470 (1995)
   PUBMED   7797537
REFERENCE   9  (bases 1 to 3023)
  AUTHORS   Shen,Z., Denison,K., Lobb,R., Gatewood,J.M. and Chen,D.J.
  TITLE     The human and mouse homologs of the yeast RAD52 gene: cDNA cloning,
            sequence analysis, assignment to human chromosome 12p12.2-p13, and
            mRNA expression in mouse tissues
  JOURNAL   Genomics 25 (1), 199-206 (1995)
   PUBMED   7774919
REFERENCE   10 (bases 1 to 3023)
  AUTHORS   Muris,D.F., Bezzubova,O., Buerstedde,J.M., Vreeken,K.,
            Balajee,A.S., Osgood,C.J., Troelstra,C., Hoeijmakers,J.H.,
            Ostermann,K., Schmidt,H. et al.
  TITLE     Cloning of human and mouse genes homologous to RAD52, a yeast gene
            involved in DNA repair and recombination
  JOURNAL   Mutat Res 315 (3), 295-305 (1994)
   PUBMED   7526206
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from HY045122.1, U12134.1,
            AC004803.11 and AA402047.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On May 17, 2019 this sequence version replaced NM_134424.3.
            
            Summary: The protein encoded by this gene shares similarity with
            Saccharomyces cerevisiae Rad52, a protein important for DNA
            double-strand break repair and homologous recombination. This gene
            product was shown to bind single-stranded DNA ends, and mediate the
            DNA-DNA interaction necessary for the annealing of complementary
            DNA strands. It was also found to interact with DNA recombination
            protein RAD51, which suggested its role in RAD51 related DNA
            recombination and repair. A pseudogene of this gene is present on
            chromosome 2. Alternative splicing results in multiple transcript
            variants. Additional alternatively spliced transcript variants of
            this gene have been described, but their full-length nature is not
            known. [provided by RefSeq, Jul 2014].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a, also known as alpha). Variants 1 and 2 encode the same isoform.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U12134.1, U27516.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000358495.8/ ENSP00000351284.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-79                HY045122.1         92-170
            80-1472             U12134.1           1-1393
            1473-2374           AC004803.11        33225-34126         c
            2375-2682           U12134.1           2291-2598
            2683-2735           AA402047.1         51-103
            2736-2881           AC004803.11        32718-32863         c
            2882-3023           AA402047.1         251-392
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.33"
     gene            1..3023
                     /gene="RAD52"
                     /note="RAD52 homolog, DNA repair protein"
                     /db_xref="GeneID:5893"
                     /db_xref="HGNC:HGNC:9824"
                     /db_xref="MIM:600392"
     exon            1..93
                     /gene="RAD52"
                     /inference="alignment:Splign:2.1.0"
     exon            94..195
                     /gene="RAD52"
                     /inference="alignment:Splign:2.1.0"
     CDS             112..1368
                     /gene="RAD52"
                     /note="isoform a is encoded by transcript variant 1;
                     recombination protein RAD52; rhabdomyosarcoma antigen
                     MU-RMS-40.23"
                     /codon_start=1
                     /product="DNA repair protein RAD52 homolog isoform a"
                     /protein_id="NP_602296.2"
                     /db_xref="CCDS:CCDS8507.2"
                     /db_xref="GeneID:5893"
                     /db_xref="HGNC:HGNC:9824"
                     /db_xref="MIM:600392"
                     /translation="MSGTEEAILGGRDSHPAAGGGSVLCFGQCQYTAEEYQAIQKALR
                     QRLGPEYISSRMAGGGQKVCYIEGHRVINLANEMFGYNGWAHSITQQNVDFVDLNNGK
                     FYVGVCAFVRVQLKDGSYHEDVGYGVSEGLKSKALSLEKARKEAVTDGLKRALRSFGN
                     ALGNCILDKDYLRSLNKLPRQLPLEVDLTKAKRQDLEPSVEEARYNSCRPNMALGHPQ
                     LQQVTSPSRPSHAVIPADQDCSSRSLSSSAVESEATHQRKLRQKQLQQQFRERMEKQQ
                     VRVSTPSAEKSEAAPPAPPVTHSTPVTVSEPLLEKDFLAGVTQELIKTLEDNSEKWAV
                     TPDAGDGVVKPSSRADPAQTSDTLALNNQMVTQNRTPHSVCHQKPQAKSGSWDLQTYS
                     ADQRTTGNWESHRKSQDMKKRKYDPS"
     exon            196..297
                     /gene="RAD52"
                     /inference="alignment:Splign:2.1.0"
     exon            298..391
                     /gene="RAD52"
                     /inference="alignment:Splign:2.1.0"
     exon            392..459
                     /gene="RAD52"
                     /inference="alignment:Splign:2.1.0"
     exon            460..578
                     /gene="RAD52"
                     /inference="alignment:Splign:2.1.0"
     exon            579..654
                     /gene="RAD52"
                     /inference="alignment:Splign:2.1.0"
     exon            655..836
                     /gene="RAD52"
                     /inference="alignment:Splign:2.1.0"
     exon            837..976
                     /gene="RAD52"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1078
                     /gene="RAD52"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1306
                     /gene="RAD52"
                     /inference="alignment:Splign:2.1.0"
     exon            1307..3023
                     /gene="RAD52"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1906..1911
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD52"
                     /note="hexamer: AATAAT"
     polyA_site      1933
                     /gene="RAD52"
                     /note="major polyA site"
     regulatory      2230..2235
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD52"
                     /note="hexamer: AATATA"
     polyA_site      2255
                     /gene="RAD52"
                     /note="major polyA site"
     regulatory      2649..2654
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD52"
                     /note="hexamer: CATAAA"
     polyA_site      2670
                     /gene="RAD52"
                     /note="major polyA site"
ORIGIN      
        1 attctcctct gcgcggcctc catctaagat ctcttcccct tgtccatagc ctagatcgag
       61 ctccctgtgt gcaccgcgcg ctgcccgagg cgcaggtcaa ccagaatcaa gatgtctggg
      121 actgaggaag caattcttgg aggacgtgac agccatcctg ctgctggcgg cggctcagtg
      181 ttatgctttg gacagtgcca gtacacagca gaagagtacc aggccatcca gaaggccctg
      241 aggcagaggc tgggcccaga atacataagt agccgcatgg ctggcggagg ccagaaggtg
      301 tgctacattg agggtcatcg ggtaattaat ctggccaatg agatgtttgg ttacaatggc
      361 tgggcacact ccatcacgca gcagaatgtg gattttgttg acctcaacaa tggcaagttc
      421 tacgtgggag tctgtgcatt tgtgagggtc cagctgaagg atggttcata tcatgaagat
      481 gttggttatg gtgttagtga gggcctcaag tccaaggctt tatctttgga gaaggcaagg
      541 aaggaggcgg tgacagacgg gctgaagcga gccctcagga gttttgggaa tgcacttgga
      601 aactgtattc tggacaaaga ctacctgaga tcactaaata agcttccacg ccagttgcct
      661 cttgaagtgg atttaactaa agcgaagaga caagatcttg aaccgtctgt ggaggaggca
      721 agatacaaca gctgccgacc gaacatggcc ctgggacacc cacagctgca gcaggtgacc
      781 tccccttcca gacccagcca tgctgtgata ccggcggacc aggactgcag ctcccgaagc
      841 ctgagctcat ccgccgtgga gagcgaggcc acgcaccagc ggaagctccg gcagaagcag
      901 ctgcagcagc agttccggga gcggatggag aagcagcagg ttcgagtctc cacgccgtca
      961 gctgagaaga gtgaggcagc gcctccggcc cctcctgtga cgcacagcac tcctgtaact
     1021 gtctcagaac cactcctgga gaaagacttc cttgcaggag tgactcaaga attaatcaag
     1081 actcttgaag acaactctga aaagtgggct gtgactcccg atgcagggga tggtgtggtc
     1141 aagccctcgt ctagagcaga cccagcccag acctctgaca cattagcctt gaacaaccag
     1201 atggtgaccc agaacaggac tccacacagc gtttgccacc agaaaccaca agcaaaatct
     1261 ggatcttggg acctccaaac ttatagcgct gaccaacgca caacaggaaa ctgggaatct
     1321 cataggaaga gccaggacat gaagaaaagg aaatatgatc catcttaact gaggctcagg
     1381 ccacataatt ggactctgtc acaaagggac tttggaaaac tactttttgg tcatgaaatt
     1441 gttcatcgct gctggagaat gaacgtcatt gcgatttatc ttgcttcatt ctgaacctta
     1501 tcaagaggat ctgactgaga gcccactgca gttagagctg agcacttttg aaaagcttgt
     1561 ccatcactct agtagggaga ggctctggac agatgaatac cttttcttcg gcttgtgagg
     1621 cttcccacta tttattactg aactattatg ttaatgaaga tggacatttt aggaatcacc
     1681 aatggctcct tgccctcaag caatataggc cagacttggt cctaagcacc tgcctcagca
     1741 attgtctaca ttcagttgtt ttgcataacg tctgccttct ttcctttacg gtccatgcct
     1801 ttaatgttgc ccacattaag cactgtggat cacgacagga aaaaggttgg agcagtgctt
     1861 ttcactactt tgtatcaatc caggctacaa tcttcattta atataaataa tttatggatt
     1921 tatgacatta caatcctgca ttgtttcaag actgacattt tttcctaagg aaggaaataa
     1981 tcatctaaga ccacgaaaaa aggctgtttt ttgttttttt tttttttttt ttttttgaga
     2041 cggggtctgg ctgtgttgcc ctgactggag ttcagtggtg caaacacagc tctctccaca
     2101 acctcttggg cccaagtgat actcccacct ctgccttaca aaatacaggg attactggtg
     2161 tgagccactg tgtctggcca gaaaaggcat ttttgagaaa gcaaatcgta taccttatta
     2221 acaaaataga atatatatat attgcttatc tgaaatgctt gaaaccagaa ttgttttgca
     2281 ttttttgaat atttgtatac acataatgag accttgggga tgggacccaa gtctgaacgt
     2341 ggaattcacc tgtgtttcgt gtatatgcct catacacata attttgtgca tgaaacagag
     2401 tttttgtata agaagataca ctgcagctga agagggctgg gttttttttt ctcttagggt
     2461 cgctgcataa actgttgtat gcctggtgct ttgcgacttg tcacacgagg tcacgtgtgg
     2521 aattttccac ttctggcatc acgtcagtgc tcagaaattt tctgatctca gagcatttca
     2581 attagggatg ctcaaacgca actgtttcta cttccccatt tcaggtgtga gatgtaaccc
     2641 accttgacca taaattggct tttcatagtg ctcagatgtt tcataattct tcccccaaaa
     2701 taatgaagtt tgcagtttaa caactttttt tttgagaggg ggggtctggc tctgtagcgc
     2761 aggctggagt gcagcggtgc catcttgact cattgcagcc tccgcctgcc aggattcaag
     2821 caattctcct gcctcaggct cccgagtagc tgggattaca gacacccacc acgacgcccg
     2881 actaattttt gtatttttag tagaggagac ggggtttctc catgttggcc aggctggtct
     2941 caaactgacc tcaagtgatc tgcccacctc ggcctcccaa agtaaaaagt actagaatta
     3001 taggcatgag ccattgcgcc cag
//
