LOCUS       NM_006479               2112 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens RAD51 associated protein 1 (RAD51AP1), transcript
            variant 2, mRNA.
ACCESSION   NM_006479
VERSION     NM_006479.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Chen,L., Gai,X. and Yu,X.
  TITLE     Pre-rRNA facilitates the recruitment of RAD51AP1 to DNA
            double-strand breaks
  JOURNAL   J Biol Chem 300 (3), 107115 (2024)
   PUBMED   38403248
  REMARK    GeneRIF: Pre-rRNA facilitates the recruitment of RAD51AP1 to DNA
            double-strand breaks.
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Wang,Y., Li,Y., Ran,C., Le,W., Dong,J., Wang,X., Chen,B. and
            Jiang,X.
  TITLE     The gene RAD51AP1 promotes the progression of pancreatic cancer via
            the PI3K/Akt/NF-kappaB signaling pathway
  JOURNAL   Neoplasma 70 (6), 722-732 (2023)
   PUBMED   37962862
  REMARK    GeneRIF: The gene RAD51AP1 promotes the progression of pancreatic
            cancer via the PI3K/Akt/NF-kappaB signaling pathway.
REFERENCE   3  (bases 1 to 2112)
  AUTHORS   Zhou,J., Tong,F., Zhao,J., Cui,X., Wang,Y., Wang,G., Kang,C.,
            Liu,X. and Wang,Q.
  TITLE     Identification of the E2F1-RAD51AP1 axis as a key factor in
            MGMT-methylated GBM TMZ resistance
  JOURNAL   Cancer Biol Med 20 (5), 385-400 (2023)
   PUBMED   37283490
  REMARK    GeneRIF: Identification of the E2F1-RAD51AP1 axis as a key factor
            in MGMT-methylated GBM TMZ resistance.
REFERENCE   4  (bases 1 to 2112)
  AUTHORS   Bridges,A.E., Ramachandran,S., Tamizhmani,K., Parwal,U., Lester,A.,
            Rajpurohit,P., Morera,D.S., Hasanali,S.L., Arjunan,P., Jedeja,R.N.,
            Patel,N., Martin,P.M., Korkaya,H., Singh,N., Manicassamy,S.,
            Prasad,P.D., Lokeshwar,V.B., Lokeshwar,B.L., Ganapathy,V. and
            Thangaraju,M.
  TITLE     RAD51AP1 Loss Attenuates Colorectal Cancer Stem Cell Renewal and
            Sensitizes to Chemotherapy
  JOURNAL   Mol Cancer Res 19 (9), 1486-1497 (2021)
   PUBMED   34099522
  REMARK    GeneRIF: RAD51AP1 Loss Attenuates Colorectal Cancer Stem Cell
            Renewal and Sensitizes to Chemotherapy.
REFERENCE   5  (bases 1 to 2112)
  AUTHORS   Wang,H., Dong,H., Qiao,L., Wu,Y., Wu,B. and Jin,X.
  TITLE     ZEB1 induces non-small cell lung cancer development by targeting
            microRNA-320a to increase the expression of RAD51AP1
  JOURNAL   Exp Cell Res 405 (2), 112687 (2021)
   PUBMED   34097859
  REMARK    GeneRIF: ZEB1 induces non-small cell lung cancer development by
            targeting microRNA-320a to increase the expression of RAD51AP1.
REFERENCE   6  (bases 1 to 2112)
  AUTHORS   Wiese,C., Dray,E., Groesser,T., San Filippo,J., Shi,I.,
            Collins,D.W., Tsai,M.S., Williams,G.J., Rydberg,B., Sung,P. and
            Schild,D.
  TITLE     Promotion of homologous recombination and genomic stability by
            RAD51AP1 via RAD51 recombinase enhancement
  JOURNAL   Mol Cell 28 (3), 482-490 (2007)
   PUBMED   17996711
  REMARK    GeneRIF: RAD51AP1 helps maintain genomic integrity via RAD51
            recombinase enhancement.
REFERENCE   7  (bases 1 to 2112)
  AUTHORS   Modesti,M., Budzowska,M., Baldeyron,C., Demmers,J.A., Ghirlando,R.
            and Kanaar,R.
  TITLE     RAD51AP1 is a structure-specific DNA binding protein that
            stimulates joint molecule formation during RAD51-mediated
            homologous recombination
  JOURNAL   Mol Cell 28 (3), 468-481 (2007)
   PUBMED   17996710
  REMARK    GeneRIF: RAD51AP1 has a selective affinity for branched-DNA
            structures that are obligatory intermediates during joint molecule
            formation.
REFERENCE   8  (bases 1 to 2112)
  AUTHORS   Kovalenko,O.V., Wiese,C. and Schild,D.
  TITLE     RAD51AP2, a novel vertebrate- and meiotic-specific protein, shares
            a conserved RAD51-interacting C-terminal domain with RAD51AP1/PIR51
  JOURNAL   Nucleic Acids Res 34 (18), 5081-5092 (2006)
   PUBMED   16990250
REFERENCE   9  (bases 1 to 2112)
  AUTHORS   Song,H., Xia,S.L., Liao,C., Li,Y.L., Wang,Y.F., Li,T.P. and
            Zhao,M.J.
  TITLE     Genes encoding Pir51, Beclin 1, RbAp48 and aldolase b are up or
            down-regulated in human primary hepatocellular carcinoma
  JOURNAL   World J Gastroenterol 10 (4), 509-513 (2004)
   PUBMED   14966907
  REMARK    GeneRIF: expression of three (Beclin 1, RbAp48 and Pir51) were
            increased and one (aldolase b) was decreased in liver tumor tissues
REFERENCE   10 (bases 1 to 2112)
  AUTHORS   Kovalenko,O.V., Golub,E.I., Bray-Ward,P., Ward,D.C. and
            Radding,C.M.
  TITLE     A novel nucleic acid-binding protein that interacts with human
            rad51 recombinase
  JOURNAL   Nucleic Acids Res 25 (24), 4946-4953 (1997)
   PUBMED   9396801
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK291948.1, BU689445.1 and AC005832.1.
            
            On Jun 2, 2019 this sequence version replaced NM_006479.4.
            
            Transcript Variant: This variant lacks an alternate in-frame exon
            compared to variant 1. The resulting isoform (b) has the same N-
            and C-termini but is shorter compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC006992.1, SRR7346977.989663.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000352618.9/ ENSP00000309479.7
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1576              AK291948.1         1-1576
            1577-1760           BU689445.1         370-553             c
            1761-1761           AC005832.1         135410-135410       c
            1762-2112           BU689445.1         18-368              c
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.32"
     gene            1..2112
                     /gene="RAD51AP1"
                     /gene_synonym="PIR51"
                     /note="RAD51 associated protein 1"
                     /db_xref="GeneID:10635"
                     /db_xref="HGNC:HGNC:16956"
                     /db_xref="MIM:603070"
     exon            1..67
                     /gene="RAD51AP1"
                     /gene_synonym="PIR51"
                     /inference="alignment:Splign:2.1.0"
     CDS             51..1058
                     /gene="RAD51AP1"
                     /gene_synonym="PIR51"
                     /note="isoform b is encoded by transcript variant 2;
                     RAD51-interacting protein; hsRAD51AP1"
                     /codon_start=1
                     /product="RAD51-associated protein 1 isoform b"
                     /protein_id="NP_006470.1"
                     /db_xref="CCDS:CCDS8529.1"
                     /db_xref="GeneID:10635"
                     /db_xref="HGNC:HGNC:16956"
                     /db_xref="MIM:603070"
                     /translation="MVRPVRHKKPVNYSQFDHSDSDDDFVSATVPLNKKSRTAPKELK
                     QDKPKPNLNNLRKEEIPVQEKTPKKRMALDDKLYQRDLEVALALSVKELPTVTTNVQN
                     SQDKSIEKHGSSKIETMNKSPHISNCSVASDYLDLDKITVEDDVGGVQGKRKAASKAA
                     AQQRKILLEGSDGDSANDTEPDFAPGEDSEDDSDFCESEDNDEDFSMRKSKVKEIKKK
                     EVKVKSPVEKKEKKSKSKCNALVTSVDSAPAAVKSESQSLPKKVSLSSDTTRKPLEIR
                     SPSAESKKPKWVPPAASGGSRSSSSPLVVVSVKSPNQSLRLGLSRLARVKPLHPNATS
                     T"
     exon            68..117
                     /gene="RAD51AP1"
                     /gene_synonym="PIR51"
                     /inference="alignment:Splign:2.1.0"
     exon            118..259
                     /gene="RAD51AP1"
                     /gene_synonym="PIR51"
                     /inference="alignment:Splign:2.1.0"
     exon            260..369
                     /gene="RAD51AP1"
                     /gene_synonym="PIR51"
                     /inference="alignment:Splign:2.1.0"
     exon            370..456
                     /gene="RAD51AP1"
                     /gene_synonym="PIR51"
                     /inference="alignment:Splign:2.1.0"
     exon            457..606
                     /gene="RAD51AP1"
                     /gene_synonym="PIR51"
                     /inference="alignment:Splign:2.1.0"
     exon            607..771
                     /gene="RAD51AP1"
                     /gene_synonym="PIR51"
                     /inference="alignment:Splign:2.1.0"
     exon            772..921
                     /gene="RAD51AP1"
                     /gene_synonym="PIR51"
                     /inference="alignment:Splign:2.1.0"
     exon            922..2112
                     /gene="RAD51AP1"
                     /gene_synonym="PIR51"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2092..2097
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD51AP1"
                     /gene_synonym="PIR51"
                     /note="hexamer: ATTAAA"
     polyA_site      2112
                     /gene="RAD51AP1"
                     /gene_synonym="PIR51"
                     /note="major polyA site"
ORIGIN      
        1 gaagccaaca agaatttgag aactgtaaat accaagcctt gaaagggacc atggtgcggc
       61 ctgtgagaca taagaaacca gtcaattact cacagtttga ccactctgac agtgatgatg
      121 attttgtttc tgcaactgta cctttaaaca agaaatccag aacagcacca aaggagttaa
      181 aacaagataa accaaaacct aacttgaaca atctccggaa agaagaaatc ccagtacaag
      241 agaaaacccc taaaaaaagg atggctttag atgacaagct ctaccagaga gacttagaag
      301 ttgcactagc tttatcagtg aaggaacttc caacagtcac cactaatgtg cagaactctc
      361 aagataaaag cattgaaaaa catggcagta gtaaaataga aacaatgaat aagtctcctc
      421 atatctctaa ttgcagtgta gccagtgatt atttagattt ggataagatt actgtggaag
      481 atgatgttgg tggtgttcaa gggaaaagaa aagcagcatc taaagctgca gcacagcaga
      541 ggaagattct tctggaaggc agtgatggtg atagtgctaa tgacactgaa ccagactttg
      601 cacctggtga agattctgag gatgattctg atttttgtga gagtgaggat aatgacgaag
      661 acttctctat gagaaaaagt aaagttaaag aaattaaaaa gaaagaagtg aaggtaaaat
      721 ccccagtaga aaagaaagag aagaaatcta aatccaaatg taatgctttg gtgacttcgg
      781 tggactctgc tccagctgcc gtcaaatcag aatctcagtc cttgccaaaa aaggtttctc
      841 tgtcttcaga taccactagg aaaccattag aaatacgcag tccttcagct gaaagcaaga
      901 aacctaaatg ggtcccacca gcggcatctg gaggtagcag aagtagcagc agcccactgg
      961 tggtagtgtc tgtgaagtct cccaatcaga gtctccgcct tggcttgtcc agattagcac
     1021 gagttaaacc tttgcatcca aatgccacta gcacctgagt gtggtacagg aggaatgttt
     1081 ggttgggaga atcacagctt tacaagggtg tttatatttg atttgtgttt atatttgagg
     1141 caggtattgt aatataaagg aatccattac catgtcctat aaatgacctc tagccatttt
     1201 atgattatgt tctctgtaaa actcttcaag acttcaatga gaagtttgtt tataagaatt
     1261 atcttctcat acctttcctt gtgaagagcg tattctgttt ttctatcagt tcgacatgaa
     1321 gtccacatca catgctgttc ttttctagtt acatgatgtg cctttctagc tttgtctagt
     1381 ttatagcacc ttaactttaa ctgttcagtt ttatctggca gaggaaaaca ttcttatttc
     1441 tttcagaaga catttctgaa atcttataag ctacttaagc tacgttgtca gttttatcgc
     1501 aaagatgttt tgtattttag ccaaatcttt ttatagtaca aacttagaat tattttacac
     1561 actaaaatgg ttgcagtttt atggcatatg tctccgattt agatggttat tctctagaaa
     1621 atagtattta aagacatttt atgaaatctt cattgtcaaa acctttaata aaagtggaaa
     1681 tattttgaaa tgcccttttt cttgatacca ctcatccacg tgttcctgat tgtccacatt
     1741 tcatgataaa atgagagctc cgcagagaat gttagccttt ctgttgtaaa tgtaatcttc
     1801 aagtagtcac tttttgttaa gttctttaga aagtagttgt caagtactta gtcatcccta
     1861 ttatgatatg agatagtaca gcttttcagg aagcttagat ctgaatttac tttgaaaaac
     1921 aattgtaatg aatattttat atttacattg agaatttcaa ctagcttctg atcaattttt
     1981 aataaaaaat tttcaaatca tgttagctgt taaaaaatgt ataataactc agtttttctt
     2041 ggtttatgga aatatctata ttaatgtgaa aataattaat ttagaattgt gattaaagtg
     2101 agcatttgtc ta
//
