LOCUS       NM_005732               8272 bp    mRNA    linear   PRI 18-NOV-2024
DEFINITION  Homo sapiens RAD50 double strand break repair protein (RAD50),
            mRNA.
ACCESSION   NM_005732
VERSION     NM_005732.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Khayat,F., Alshmery,M., Pal,M., Oliver,A.W. and Bianchi,A.
  TITLE     Binding of the TRF2 iDDR motif to RAD50 highlights a convergent
            evolutionary strategy to inactivate MRN at telomeres
  JOURNAL   Nucleic Acids Res 52 (13), 7704-7719 (2024)
   PUBMED   38884214
  REMARK    GeneRIF: Binding of the TRF2 iDDR motif to RAD50 highlights a
            convergent evolutionary strategy to inactivate MRN at telomeres.
REFERENCE   2  (bases 1 to 8272)
  AUTHORS   Chen,H., Li,Y., Li,H., Chen,X., Fu,H., Mao,D., Chen,W., Lan,L.,
            Wang,C., Hu,K., Li,J., Zhu,C., Evans,I., Cheung,E., Lu,D., He,Y.,
            Behrens,A., Yin,D. and Zhang,C.
  TITLE     NBS1 lactylation is required for efficient DNA repair and
            chemotherapy resistance
  JOURNAL   Nature 631 (8021), 663-669 (2024)
   PUBMED   38961290
REFERENCE   3  (bases 1 to 8272)
  AUTHORS   Wu,M., Jin,M.M., Cao,X.H., Zhao,L. and Li,Y.H.
  TITLE     Silencing TRIM29 Sensitizes Non-small Cell Lung Cancer Cells to
            Anlotinib by Promoting Apoptosis via Binding RAD50
  JOURNAL   Curr Cancer Drug Targets 24 (4), 445-454 (2024)
   PUBMED   37644752
  REMARK    GeneRIF: Silencing TRIM29 Sensitizes Non-small Cell Lung Cancer
            Cells to Anlotinib by Promoting Apoptosis via Binding RAD50.
REFERENCE   4  (bases 1 to 8272)
  AUTHORS   Takagi,M., Hoshino,A., Bousset,K., Roddecke,J., Martin,H.L.,
            Folcut,I., Tomomasa,D., Yang,X., Kobayashi,J., Sakata,N.,
            Yoshida,K., Miyano,S., Ogawa,S., Kojima,S., Morio,T., Dork,T. and
            Kanegane,H.
  TITLE     Bone Marrow Failure and Immunodeficiency Associated with Human
            RAD50 Variants
  JOURNAL   J Clin Immunol 43 (8), 2136-2145 (2023)
   PUBMED   37794136
  REMARK    GeneRIF: Bone Marrow Failure and Immunodeficiency Associated with
            Human RAD50 Variants.
REFERENCE   5  (bases 1 to 8272)
  AUTHORS   Gut,F., Kashammer,L., Lammens,K., Bartho,J.D., Boggusch,A.M., van
            de Logt,E., Kessler,B. and Hopfner,K.P.
  TITLE     Structural mechanism of endonucleolytic processing of blocked DNA
            ends and hairpins by Mre11-Rad50
  JOURNAL   Mol Cell 82 (18), 3513-3522 (2022)
   PUBMED   35987200
  REMARK    GeneRIF: Structural mechanism of endonucleolytic processing of
            blocked DNA ends and hairpins by Mre11-Rad50.
REFERENCE   6  (bases 1 to 8272)
  AUTHORS   Trujillo,K.M., Yuan,S.S., Lee,E.Y. and Sung,P.
  TITLE     Nuclease activities in a complex of human recombination and DNA
            repair factors Rad50, Mre11, and p95
  JOURNAL   J Biol Chem 273 (34), 21447-21450 (1998)
   PUBMED   9705271
REFERENCE   7  (bases 1 to 8272)
  AUTHORS   Paull,T.T. and Gellert,M.
  TITLE     The 3' to 5' exonuclease activity of Mre 11 facilitates repair of
            DNA double-strand breaks
  JOURNAL   Mol Cell 1 (7), 969-979 (1998)
   PUBMED   9651580
REFERENCE   8  (bases 1 to 8272)
  AUTHORS   Carney,J.P., Maser,R.S., Olivares,H., Davis,E.M., Le Beau,M.,
            Yates,J.R. 3rd, Hays,L., Morgan,W.F. and Petrini,J.H.
  TITLE     The hMre11/hRad50 protein complex and Nijmegen breakage syndrome:
            linkage of double-strand break repair to the cellular DNA damage
            response
  JOURNAL   Cell 93 (3), 477-486 (1998)
   PUBMED   9590181
REFERENCE   9  (bases 1 to 8272)
  AUTHORS   Maser,R.S., Monsen,K.J., Nelms,B.E. and Petrini,J.H.
  TITLE     hMre11 and hRad50 nuclear foci are induced during the normal
            cellular response to DNA double-strand breaks
  JOURNAL   Mol Cell Biol 17 (10), 6087-6096 (1997)
   PUBMED   9315668
REFERENCE   10 (bases 1 to 8272)
  AUTHORS   Dolganov,G.M., Maser,R.S., Novikov,A., Tosto,L., Chong,S.,
            Bressan,D.A. and Petrini,J.H.
  TITLE     Human Rad50 is physically associated with human Mre11:
            identification of a conserved multiprotein complex implicated in
            recombinational DNA repair
  JOURNAL   Mol Cell Biol 16 (9), 4832-4841 (1996)
   PUBMED   8756642
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC140005.1, AC116366.2,
            U63139.1 and AC004041.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 3, 2019 this sequence version replaced NM_005732.3.
            
            Summary: The protein encoded by this gene is highly similar to
            Saccharomyces cerevisiae Rad50, a protein involved in DNA
            double-strand break repair. This protein forms a complex with MRE11
            and NBS1. The protein complex binds to DNA and displays numerous
            enzymatic activities that are required for nonhomologous joining of
            DNA ends. This protein, cooperating with its partners, is important
            for DNA double-strand break repair, cell cycle checkpoint
            activation, telomere maintenance, and meiotic recombination.
            Knockout studies of the mouse homolog suggest this gene is
            essential for cell growth and viability. Mutations in this gene are
            the cause of Nijmegen breakage syndrome-like disorder.[provided by
            RefSeq, Apr 2010].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U63139.1, BC136436.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000378823.8/ ENSP00000368100.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-310               BC140005.1         48-357
            311-311             AC116366.2         168844-168844
            312-671             BC140005.1         359-718
            672-5830            U63139.1           712-5870
            5831-8272           AC004041.1         62971-65412
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q31.1"
     gene            1..8272
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /note="RAD50 double strand break repair protein"
                     /db_xref="GeneID:10111"
                     /db_xref="HGNC:HGNC:9816"
                     /db_xref="MIM:604040"
     exon            1..477
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            478..561
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            562..713
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            714..899
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            900..1104
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            1105..1233
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1399
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1593
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            1594..1800
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            1801..1983
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            1984..2141
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            2142..2317
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            2318..2555
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            2556..2745
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            2746..2872
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            2873..3066
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            3067..3177
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            3178..3270
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            3271..3384
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            3385..3512
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            3513..3737
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            3738..3823
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            3824..3966
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            3967..4100
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     exon            4101..8272
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4380..4385
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /note="hexamer: AATATA"
     polyA_site      4399
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /note="major polyA site"
     regulatory      5797..5802
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /note="hexamer: AATAAA"
     polyA_site      5819
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
     regulatory      5956..5961
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /note="hexamer: AATAAA"
     polyA_site      5976
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
     regulatory      6500..6505
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /note="hexamer: ACTAAA"
     regulatory      6522..6527
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /note="hexamer: AAGAAA"
     polyA_site      6529
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
     polyA_site      6544
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
     regulatory      8242..8247
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
                     /note="hexamer: AATAAA"
     polyA_site      8272
                     /gene="RAD50"
                     /gene_synonym="hRad50; NBSLD; RAD502"
ORIGIN      
        1 acgtgatccg cagggcggcc gaggcaggaa gctgtgagtg cgcggttgcg gggtcgcatt
       61 gtggctacgg ctttgcgtcc ccggcgggca gccccaggct ggtccccgcc tccgctctcc
      121 ccaccggcgg ggaaagcagc tggtgtggga ggaaaggctc catcccccgc cccctctctc
      181 ccgctgttgg ctggcaggat cttttggcag tcctgtggcc tcgctccccg cccggatcct
      241 cctgaccctg agattcgcgg gtctcacgtc ccgtgcacgc cttgcttcgg cctcagttaa
      301 gcctttgtgg gctccaggtc cctggtgaga ttagaaacgt ttgcaaacat gtcccggatc
      361 gaaaagatga gcattctggg cgtgcggagt tttggaatag aggacaaaga taagcaaatt
      421 atcactttct tcagccccct tacaattttg gttggaccca atggggcggg aaagacgacc
      481 atcattgaat gtctaaaata tatttgtact ggagatttcc ctcctggaac caaaggaaat
      541 acatttgtac acgatcccaa ggttgctcaa gaaacagatg tgagagccca gattcgtctg
      601 caatttcgtg atgtcaatgg agaacttata gctgtgcaaa gatctatggt gtgtactcag
      661 aaaagcaaaa agacagaatt taaaactctg gaaggagtca ttactagaac aaagcatggt
      721 gaaaaggtca gtctgagctc taagtgtgca gaaattgacc gagaaatgat cagttctctt
      781 ggggtttcca aggctgtgct aaataatgtc attttctgtc atcaagaaga ttctaattgg
      841 cctttaagtg aaggaaaggc tttgaagcaa aagtttgatg agattttttc agcaacaaga
      901 tacattaaag ccttagaaac acttcggcag gtacgtcaga cacaaggtca gaaagtaaaa
      961 gaatatcaaa tggaactaaa atatctgaag caatataagg aaaaagcttg tgagattcgt
     1021 gatcagatta caagtaagga agcccagtta acatcttcaa aggaaattgt caaatcctat
     1081 gagaatgaac ttgatccatt gaagaatcgt ctaaaagaaa ttgaacataa tctctctaaa
     1141 ataatgaaac ttgacaatga aattaaagcc ttggatagcc gaaagaagca aatggagaaa
     1201 gataatagtg aactggaaga gaaaatggaa aaggtttttc aagggactga tgagcaacta
     1261 aatgacttat atcacaatca ccagagaaca gtaagggaga aagaaaggaa attggtagac
     1321 tgtcatcgtg aactggaaaa actaaataaa gaatctaggc ttctcaatca ggaaaaatca
     1381 gaactgcttg ttgaacaggg tcgtctacag ctgcaagcag atcgccatca agaacatatc
     1441 cgagctagag attcattaat tcagtctttg gcaacacagc tagaattgga tggctttgag
     1501 cgtggaccat tcagtgaaag acagattaaa aattttcaca aacttgtgag agagagacaa
     1561 gaaggggaag caaaaactgc caaccaactg atgaatgact ttgcagaaaa agagactctg
     1621 aaacaaaaac agatagatga gataagagat aagaaaactg gactgggaag aataattgag
     1681 ttaaaatcag aaatcctaag taagaagcag aatgagctga aaaatgtgaa gtatgaatta
     1741 cagcagttgg aaggatcttc agacaggatt cttgaactgg accaggagct cataaaagct
     1801 gaacgtgagt taagcaaggc tgagaaaaac agcaatgtag aaaccttaaa aatggaagta
     1861 ataagtctcc aaaatgaaaa agcagactta gacaggaccc tgcgtaaact tgaccaggag
     1921 atggagcagt taaaccatca tacaacaaca cgtacccaaa tggagatgct gaccaaagac
     1981 aaagctgaca aagatgaaca aatcagaaaa ataaaatcta ggcacagtga tgaattaacc
     2041 tcactgttgg gatattttcc caacaaaaaa cagcttgaag actggctaca tagtaaatca
     2101 aaagaaatta atcagaccag ggacagactt gccaaattga acaaggaact agcttcatct
     2161 gagcagaata aaaatcatat aaataatgaa ctaaaaagaa aggaagagca gttgtccagt
     2221 tacgaagaca agctgtttga tgtttgtggt agccaggatt ttgaaagtga tttagacagg
     2281 cttaaagagg aaattgaaaa atcatcaaaa cagcgagcca tgctggctgg agccacagca
     2341 gtttactccc agttcattac tcagctaaca gacgaaaacc agtcatgttg ccccgtttgt
     2401 cagagagttt ttcagacaga ggctgagtta caagaagtca tcagtgattt gcagtctaaa
     2461 ctgcgacttg ctccagataa actcaagtca acagaatcag agctaaaaaa aaaggaaaag
     2521 cggcgtgatg aaatgctggg acttgtgccc atgaggcaaa gcataattga tttgaaggag
     2581 aaggaaatac cagaattaag aaacaaactg cagaatgtca atagagacat acagcgccta
     2641 aagaacgaca tagaagaaca agaaacactc ttgggtacaa taatgcctga agaagaaagt
     2701 gccaaagtat gcctgacaga tgttacaatt atggagaggt tccagatgga acttaaagat
     2761 gttgaaagaa aaattgcaca acaagcagct aagctacaag gaatagactt agatcgaact
     2821 gtccaacaag tcaaccagga gaaacaagag aaacagcaca agttagacac agtttctagt
     2881 aagattgaat tgaatcgtaa gcttatacag gaccagcagg aacagattca acatctaaaa
     2941 agtacaacaa atgagctaaa atctgagaaa cttcagatat ccactaattt gcaacgtcgt
     3001 cagcaactgg aggagcagac tgtggaatta tccactgaag ttcagtcttt gtacagagag
     3061 ataaaggatg ctaaagagca ggtaagccct ttggaaacaa cattggaaaa gttccagcaa
     3121 gaaaaagaag aattaatcaa caaaaaaaat acaagcaaca aaatagcaca ggataaactg
     3181 aatgatatta aagagaaggt taaaaatatt catggctata tgaaagacat tgagaattat
     3241 attcaagatg ggaaagacga ctataagaag caaaaagaaa ctgaacttaa taaagtaata
     3301 gctcaactaa gtgaatgcga gaaacacaaa gaaaagataa atgaagatat gagactcatg
     3361 agacaagata ttgatacaca gaagatacaa gaaaggtggc tacaagataa ccttacttta
     3421 agaaaaagaa atgaggaact aaaagaagtt gaagaagaaa gaaaacaaca tttgaaggaa
     3481 atgggtcaaa tgcaggtttt gcaaatgaaa agtgaacatc agaagttgga agagaacata
     3541 gacaatataa aaagaaatca taatttggca ttagggcgac agaaaggtta tgaagaagaa
     3601 attattcatt ttaagaaaga acttcgagaa ccacaatttc gggatgctga ggaaaagtat
     3661 agagaaatga tgattgttat gaggacaaca gaacttgtga acaaggatct ggatatttat
     3721 tataagactc ttgaccaagc aataatgaaa tttcacagta tgaaaatgga agaaatcaat
     3781 aaaattatac gtgacctgtg gcgaagtacc tatcgtggac aagatattga atacatagaa
     3841 atacggtctg atgccgatga aaatgtatca gcttctgata aaaggcggaa ttataactac
     3901 cgagtggtga tgctgaaggg agacacagcc ttggatatgc gaggacgatg cagtgctgga
     3961 caaaaggtat tagcctcact catcattcgc ctggccctgg ctgaaacgtt ctgcctcaac
     4021 tgtggcatca ttgccttgga tgagccaaca acaaatcttg accgagaaaa cattgaatct
     4081 cttgcacatg ctctggttga gataataaaa agtcgctcac agcagcgtaa cttccagctt
     4141 ctggtaatca ctcatgatga agattttgtg gagcttttag gacgttctga atatgtggag
     4201 aaattctaca ggattaaaaa gaacatcgat cagtgctcag agattgtgaa atgcagtgtt
     4261 agctccctgg gattcaatgt tcattaaaaa tatccaagat ttaaatgcca tagaaatgta
     4321 ggtcctcaga aagtgtataa taagaaactt atttctcata tcaacttagt caataagaaa
     4381 atatattctt tcaaaggaac attgtgtcta ggattttgga tgttgagagg ttctaaaatc
     4441 atgaaacttg tttcactgaa aattggacag attgcctgtt tctgatttgc tgctcttcat
     4501 cccattccag gcagcctctg tcaggccttc agggttcagc agtacagccg agactcgact
     4561 ctgtgcctcc ctccccagtg caaatgcatg cttcttctca aagcactgtt gagaaggaga
     4621 taattactgc cttgaaaatt tatggttttg gtattttttt aaatcatagt taaatgttac
     4681 ctctgaattt acttccttgc atgtggtttg aaaaactgag tattaatatc tgaggatgac
     4741 cagaaatggt gagatgtatg tttggctctg cttttaactt tataaatcca gtgacctctc
     4801 tctctgggac ttggtttccc caactaaaat ttgaagtagt tgaatggggt ctcaaagttt
     4861 gacaggaacc ttaagtaatc atctaagtca gtacccacca ccttcttctc ctacatatcc
     4921 cttccagatg gtcatccaga ctcagagctc tctctacaga gaggaaattc tccactgtgc
     4981 acacccacct ttggaaagct ctgaccactt gaggcctgat ctgcccatcg tgaagaagcc
     5041 tgtaacactc ctctgcgtct atcctgtgta gcatactggc ttcaccatca atcctgattc
     5101 ctctctaagt gggcattgcc atgtggaagg caagccaggc tcactcacag agtcaaggcc
     5161 tgctccctgt agggtccaac cagacctgga agaacaggcc tctccatttg ctcttcagat
     5221 gccacttcta agaaaagcct aatcacagtt tttcctggaa ttgccagctg acatcttgaa
     5281 tccttccatt ccacacagaa tgcaaccaag tcacacgctt ttgaattatg ctttgtagag
     5341 ttttgtcatt cagagtcagc caggaccata ccgggtcttg attcagtcac atggcatggt
     5401 tttgtgccat ctgtagctat aatgagcatg tttgcctaga cagcttttct caactgggtc
     5461 cagaagagaa ttaagcccta aggtcctaag gcatctatct gtgctaggtt aaatggttgg
     5521 cccccaaaga tagacaggtc ctgatttcta gaacccgtga ctgttacttt atacagcaaa
     5581 ggaaactttg cagatgtgat taaagctaag gaccttaaga cagagtatcc tgggggtggt
     5641 ggtggggtgg gggggggtcc taaatgtaat cacgagtaag attaagagca aatcaattct
     5701 agtcatatat taaacatcca caataaccaa gatattttta tcccaagaat gcaagatttc
     5761 agaaaatgaa aaatctgttg ataaatccat cactataata aaaccgaagg tgaaaaaaat
     5821 tctgaaaaaa ttctagcagc tatatttgat aaaattcaac atctcctagc tttagcaaac
     5881 tcacagtttt gcaaataata ttttcttaat gttatctgtt gctaaatcaa aattaaacag
     5941 tcatcttaac tgcaaaataa aacatttctc agtaaatatt aaagccagtt accttctatc
     6001 aacatgttaa tgaaagtgct agttgttgca gcaaagaata acaaaggcaa tacacgatca
     6061 atataggcag tgaaacaaaa gtatcatttg caagttaaaa cagacttccc aattttaaat
     6121 ctggtttccc cctgaatatg tggcatcctt ggcagcactt ctgagagtgg ctgctttcat
     6181 tccaagaagc ccatgggttt ggaggtggga taggtgcctt tctggcttct cattgctgct
     6241 tctagatcag tctccaaata tcccccttcc ccacattgga atgaatagcc atcacagcat
     6301 ggatggaggt tagaatgagc cagactgcct gggctcaaat cctagcacac cactcactag
     6361 ctggggacct tgagcaagtt atttgtcctg ttttctgttt ccttatatgt aaaagtgggt
     6421 aaaatggtac atattttgta gggttgttat gaagattgaa tgacattatt tacaaactgc
     6481 ttagaactgc ttgccaccta ctaaatactg tgtaagtgtt caagaaaaag ctgtcttcat
     6541 ttcactcttg ttgcagttat ctttcattcc cagcttggtc actccctctt atttgttgaa
     6601 gagtttagct cccggttcag tgtcacccca ttactaataa tcattgctga tttcaatatc
     6661 caagtgatgg tctaatacaa ctccttggcc tctcagtctt ttttattttt tgaggtggag
     6721 tcttgctctg tcacccaggc tggagtgcaa tggcaccatc tcagctcact gcaacctctg
     6781 cctccctgat acaagcaatc ctcctgcctc agcctcccaa gtagctagga ttacaggcat
     6841 gtaccaccac accaggctaa tttttgtatt ttagtagaga cagggtttcg ccttgttggc
     6901 caggctggtc ttgaacgtct ggcctcaagt gatctgccta ccttggcctc cctcagcccc
     6961 tttttgtttc ctccagtgag aagatactca gttctatggt cttattctag agccccgtcc
     7021 ttttaataac aataactgca cccctccaaa atctcagttt taagcatctc actctccaat
     7081 aaccccatgg tgtctacaat tttcctgtgg tcctcaaccc cagcaattct ttgaccccac
     7141 tgagatttca atccattgat tctatggctt tttcacaggt tgtttccctg atgcctgtac
     7201 ttctctcctt attcaactta aattccatgg ctcatcatta tgtcaccctc cactgcattg
     7261 tactcaaact gtcccgacaa tccccagccc tactggaatc cagctctgcc tcctccctgc
     7321 aacacctgca catcccgttt cctttgctgt ttcctcctca tttccctggc ccctcaatgt
     7381 cacaggtttt agtactcaga gtctgcacct cttttcttct ctgtgctcac ttatttggtg
     7441 atctcatcca gtctcatagc tttaaattat cttagtttgg gttctcccag aagcagagcg
     7501 taaaacaatg cttcacgtga aagtaatcag taggaaattg gggaaattgt gtatgaaaga
     7561 gaaagcagcc aatgaaaagt ttactattaa gccagctacg gtaagtgggc aattagagtt
     7621 tggtgcccca gggagactct ggggagtttg gcaaaatata tacctcagaa ttatcccacc
     7681 taaggggcaa gagacctgga gtatttatat aacaacttgc cagggttact gagtgaaggc
     7741 tattctagca catgttagtt actggcttgc catgtgtacc gacacagctg ctttccacag
     7801 tttaaaaaga aagtctgagc aacatagtga catcccatct ttacataaaa tttttaaaaa
     7861 attagcaagg catggtggtg cacacctgta gtctcagcta cttggctgag ataggatccc
     7921 tggagtctga gggttcaagg ctgcagtgag ctgtgatttg tgccactgca ccccagcctg
     7981 ggtgggtggc agagtgagac cctgtctaaa aagacaaaaa aaaaaaaaaa aaaaaagcag
     8041 cagcagctga aagttggcag gagcacactg gcagttccta ggatcatagg atataggcag
     8101 ggcactgacc actcctacaa ccaccatcta tacactgata actcataaat gtttgtgtcc
     8161 aatccagatc tctcctaaat gcttgactga tatattcagc tgcctgtgga taactgggca
     8221 tctatctgca cttgaatatc taataaacgt ctaaaactta aaatgaccaa aa
//
