LOCUS       NM_020165               5857 bp    mRNA    linear   PRI 06-JUN-2024
DEFINITION  Homo sapiens RAD18 E3 ubiquitin protein ligase (RAD18), mRNA.
ACCESSION   NM_020165
VERSION     NM_020165.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Jiang,B., Yan,B., Yang,H., Geng,H. and Li,P.
  TITLE     Transcription Factor E2F7 Hampers the Killing Effect of NK Cells
            against Colorectal Cancer Cells via Activating RAD18 Transcription
  JOURNAL   J Microbiol Biotechnol 34 (4), 920-929 (2024)
   PUBMED   38073330
  REMARK    GeneRIF: Transcription Factor E2F7 Hampers the Killing Effect of NK
            Cells against Colorectal Cancer Cells via Activating RAD18
            Transcription.
REFERENCE   2  (bases 1 to 5857)
  AUTHORS   Shimada,M., Tokumiya,T., Miyake,T., Tsukada,K., Kanzaki,N.,
            Yanagihara,H., Kobayashi,J. and Matsumoto,Y.
  TITLE     Implication of E3 ligase RAD18 in UV-induced mutagenesis in human
            induced pluripotent stem cells and neuronal progenitor cells
  JOURNAL   J Radiat Res 64 (2), 345-351 (2023)
   PUBMED   36634340
  REMARK    GeneRIF: Implication of E3 ligase RAD18 in UV-induced mutagenesis
            in human induced pluripotent stem cells and neuronal progenitor
            cells.
REFERENCE   3  (bases 1 to 5857)
  AUTHORS   Wells,J.P., Chang,E.Y., Dinatto,L., White,J., Ryall,S. and
            Stirling,P.C.
  TITLE     RAD18 opposes transcription-associated genome instability through
            FANCD2 recruitment
  JOURNAL   PLoS Genet 18 (12), e1010309 (2022)
   PUBMED   36480547
  REMARK    GeneRIF: RAD18 opposes transcription-associated genome instability
            through FANCD2 recruitment.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5857)
  AUTHORS   Yu,R., Hu,Y., Zhang,S., Li,X., Tang,M., Yang,M., Wu,X., Li,Z.,
            Liao,X., Xu,Y., Li,M., Chen,S., Qian,W., Gong,L.Y., Song,L. and
            Li,J.
  TITLE     LncRNA CTBP1-DT-encoded microprotein DDUP sustains DNA damage
            response signalling to trigger dual DNA repair mechanisms
  JOURNAL   Nucleic Acids Res 50 (14), 8060-8079 (2022)
   PUBMED   35849344
REFERENCE   5  (bases 1 to 5857)
  AUTHORS   Du,M., Gu,J., Liu,C., Liu,N., Yu,Z., Zhou,C., Heng,W., Cao,Z.,
            Wei,F., Zhu,K., Wang,Y., Zhang,W., Xue,X., Zhang,Y. and Qian,J.
  TITLE     Genome-wide CRISPR screen identified Rad18 as a determinant of
            doxorubicin sensitivity in osteosarcoma
  JOURNAL   J Exp Clin Cancer Res 41 (1), 154 (2022)
   PUBMED   35459258
  REMARK    GeneRIF: Genome-wide CRISPR screen identified Rad18 as a
            determinant of doxorubicin sensitivity in osteosarcoma.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5857)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
REFERENCE   7  (bases 1 to 5857)
  AUTHORS   Nikiforov,A.A., Sasina,L.K., Svetlova,M.P., Solovjeva,L.V.,
            Oei,S.L., Bradbury,E.M. and Tomilin,N.V.
  TITLE     Early immobilization of nuclease FEN1 and accumulation of hRAD18
            protein at stalled DNA replication forks in mammalian cells
  JOURNAL   Dokl Biochem Biophys 389, 122-125 (2003)
   PUBMED   12856420
  REMARK    GeneRIF: action at DNA replication forks
REFERENCE   8  (bases 1 to 5857)
  AUTHORS   Mulder,L.C., Chakrabarti,L.A. and Muesing,M.A.
  TITLE     Interaction of HIV-1 integrase with DNA repair protein hRad18
  JOURNAL   J Biol Chem 277 (30), 27489-27493 (2002)
   PUBMED   12016221
  REMARK    GeneRIF: hRad18 is a novel interacting partner of HIV-1 integrase;
            role for post-replication/translesion DNA repair in the retroviral
            integration process.
REFERENCE   9  (bases 1 to 5857)
  AUTHORS   Xin,H., Lin,W., Sumanasekera,W., Zhang,Y., Wu,X. and Wang,Z.
  TITLE     The human RAD18 gene product interacts with HHR6A and HHR6B
  JOURNAL   Nucleic Acids Res 28 (14), 2847-2854 (2000)
   PUBMED   10908344
REFERENCE   10 (bases 1 to 5857)
  AUTHORS   Tateishi,S., Sakuraba,Y., Masuyama,S., Inoue,H. and Yamaizumi,M.
  TITLE     Dysfunction of human Rad18 results in defective postreplication
            repair and hypersensitivity to multiple mutagens
  JOURNAL   Proc Natl Acad Sci U S A 97 (14), 7927-7932 (2000)
   PUBMED   10884424
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DW433240.1, AK023075.1,
            AC034186.5 and AC008151.1.
            
            On Nov 23, 2018 this sequence version replaced NM_020165.3.
            
            Summary: The protein encoded by this gene is highly similar to S.
            cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions
            through its interaction with Rad6, which is an
            ubiquitin-conjugating enzyme required for post-replication repair
            of damaged DNA. Similar to its yeast counterpart, this protein is
            able to interact with the human homolog of yeast Rad6 protein
            through a conserved ring-finger motif.  Mutation of this motif
            results in defective replication of UV-damaged DNA and
            hypersensitivity to multiple mutagens. [provided by RefSeq, Jul
            2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660807.211327.1,
                                           SRR1660807.200457.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000264926.7/ ENSP00000264926.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-244               DW433240.1         20-263
            245-991             AK023075.1         235-981
            992-992             AC034186.5         136956-136956
            993-2561            AK023075.1         983-2551
            2562-5857           AC008151.1         151940-155235       c
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p25.3"
     gene            1..5857
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /note="RAD18 E3 ubiquitin protein ligase"
                     /db_xref="GeneID:56852"
                     /db_xref="HGNC:HGNC:18278"
                     /db_xref="MIM:605256"
     exon            1..138
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /inference="alignment:Splign:2.1.0"
     CDS             88..1575
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /EC_number="2.3.2.27"
                     /note="postreplication repair protein hRAD18p; RAD18, S.
                     cerevisiae, homolog; hHR18; RING finger protein 73;
                     postreplication repair protein RAD18; RAD18 homolog;
                     RING-type E3 ubiquitin transferase RAD18"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase RAD18"
                     /protein_id="NP_064550.3"
                     /db_xref="CCDS:CCDS2571.1"
                     /db_xref="GeneID:56852"
                     /db_xref="HGNC:HGNC:18278"
                     /db_xref="MIM:605256"
                     /translation="MDSLAESRWPPGLAVMKTIDDLLRCGICFEYFNIAMIIPQCSHN
                     YCSLCIRKFLSYKTQCPTCCVTVTEPDLKNNRILDELVKSLNFARNHLLQFALESPAK
                     SPASSSSKNLAVKVYTPVASRQSLKQGSRLMDNFLIREMSGSTSELLIKENKSKFSPQ
                     KEASPAAKTKETRSVEEIAPDPSEAKRPEPPSTSTLKQVTKVDCPVCGVNIPESHINK
                     HLDSCLSREEKKESLRSSVHKRKPLPKTVYNLLSDRDLKKKLKEHGLSIQGNKQQLIK
                     RHQEFVHMYNAQCDALHPKSAAEIVREIENIEKTRMRLEASKLNESVMVFTKDQTEKE
                     IDEIHSKYRKKHKSEFQLLVDQARKGYKKIAGMSQKTVTITKEDESTEKLSSVCMGQE
                     DNMTSVTNHFSQSKLDSPEELEPDREEDSSSCIDIQEVLSSSESDSCNSSSSDIIRDL
                     LEEEEAWEASHKNDLQDTEISPRQNRRTRAAESAEIEPRNKRNRN"
     exon            139..220
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /inference="alignment:Splign:2.1.0"
     exon            221..282
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /inference="alignment:Splign:2.1.0"
     exon            283..353
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /inference="alignment:Splign:2.1.0"
     exon            354..691
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /inference="alignment:Splign:2.1.0"
     exon            692..791
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /inference="alignment:Splign:2.1.0"
     exon            792..976
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /inference="alignment:Splign:2.1.0"
     exon            977..1053
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /inference="alignment:Splign:2.1.0"
     exon            1054..1114
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /inference="alignment:Splign:2.1.0"
     exon            1115..1255
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..1409
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /inference="alignment:Splign:2.1.0"
     exon            1410..1472
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /inference="alignment:Splign:2.1.0"
     exon            1473..5857
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2320..2325
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /note="hexamer: AACAAA"
     polyA_site      2339
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /note="major polyA site"
     regulatory      3035..3040
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /note="hexamer: ATTAAA"
     polyA_site      3062
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /note="major polyA site"
     regulatory      5715..5720
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /note="hexamer: AATAAA"
     polyA_site      5736
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /note="major polyA site"
     regulatory      5839..5844
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD18"
                     /gene_synonym="RNF73"
                     /note="hexamer: AATAAA"
     polyA_site      5857
                     /gene="RAD18"
                     /gene_synonym="RNF73"
ORIGIN      
        1 gtaatgcggt agcgcgggga atttcgagtg gtgttggagc gccggaggct agtgggtggc
       61 tgacccccag catcctcggg agcgaccatg gactccctgg ccgagtctcg gtggcctccg
      121 ggcctggcag tcatgaagac aatagatgat ttgctgcggt gtggaatttg cttcgagtat
      181 ttcaacattg caatgataat acctcagtgt tcacataact actgctctct ctgtataaga
      241 aaatttctgt cctataaaac tcagtgtcca acttgctgtg tgactgtcac agagccggat
      301 ctgaaaaata accgcatatt agatgaactg gtaaaaagct tgaattttgc acggaatcat
      361 ctgctgcagt ttgctttaga gtcaccagcc aaatctcctg cttcttcctc ttcaaagaat
      421 cttgctgtca aagtatatac tcctgtagcc tccagacagt ctttaaagca ggggagcagg
      481 ttaatggata atttcttgat cagagaaatg agtggttcta catcagagtt gttgataaaa
      541 gaaaataaaa gcaaattcag ccctcaaaaa gaggcgagcc ctgctgcaaa gaccaaagag
      601 acacgttctg tagaagagat cgctccagat ccctcagagg ctaagcgtcc tgagccaccc
      661 tcgacatcca ctttgaaaca agttactaaa gtggattgtc ctgtttgcgg ggttaacatt
      721 ccagaaagtc acattaataa gcatttagac agctgtttat cacgcgaaga gaagaaggaa
      781 agcctcagaa gttctgttca caaaaggaag ccgctgccca aaactgtata taatttgctc
      841 tctgatcgtg atttaaagaa aaagctaaaa gagcatggat tatctattca aggaaataaa
      901 caacagctca ttaaaaggca ccaagaattt gtacacatgt acaatgccca atgcgatgct
      961 ttgcatccta aatcagctgc tgaaatagtt cgagaaatcg aaaatataga gaagactagg
     1021 atgcgtcttg aagctagtaa actcaatgaa agtgtaatgg tttttacaaa ggaccaaaca
     1081 gaaaaggaaa tagatgaaat ccacagtaaa tatcgtaaaa aacataagag tgaatttcag
     1141 cttctggtgg atcaggctag aaaaggatac aagaaaattg ctggaatgtc acaaaaaaca
     1201 gtaacaataa caaaagaaga tgaatctaca gaaaagctat cttctgtatg catgggacag
     1261 gaagataata tgacctcagt aacaaaccac ttttctcaat caaagctgga ctccccagag
     1321 gaattggaac ctgacagaga agaggattct tctagctgta ttgatattca agaagttctt
     1381 tcttcatcag aatcagattc atgcaatagt tccagttcag acatcataag agatctttta
     1441 gaagaagagg aagcctggga agcatcacat aaaaacgatc ttcaagacac agaaataagt
     1501 ccaagacaga atcgccgcac aagagccgct gaaagtgctg agattgaacc aagaaacaag
     1561 cgtaatagga attaatgtgg gcttttgctg acttttcaaa tgcattgatt agaataccgt
     1621 acttttggtt gccacagata gattttctat ttataaatgc ccaaggaaag atgctaaatt
     1681 ctaaatatta cggttagctg atattcattc ttttctgctt ttccagaggg gaaaaatgtt
     1741 acaaaatatc cttacttggt cagttgcctc ctgcctctaa aacatctctc tctaaaaata
     1801 ctgacatttc acacaggtac cagctttgca gaggaggtag actctttggc actttggcac
     1861 agggatttgg tttggtttgg tttggtttga taattaaatt tcagaatgtc cttaggccat
     1921 tctccttctc ttccatggag aatccagcct cacaaaagga ttctttcaac ttctttattg
     1981 caagaaacag ttgagttaaa tgtttgtttt tggaaaggcg ggatgtcagt tcacatcctt
     2041 ggtgtgtgta agtaccgatg cacgccacca ccatgcctgt gttacagcag ctccatgatg
     2101 gttgttgccc gaggttaatg tagttgtttg ttagacctgt gtcttacaca tttctcagag
     2161 taggatatta gtattataat ttaaagctac gacagtcaca aagtcacaat aacttagaaa
     2221 cattgcgcat attcttctga aatagcactt aaaaatgatt agtgtcagta ttttttcact
     2281 tgggtcaatc aaatctgtaa cactgaatcc aagctattaa acaaaaagta tgcaatgaat
     2341 gaattttgta aatgaataga gagtatcagt ttacaataat gttcttaaaa cagtatcctc
     2401 tggatagttg agtatgggtt agaaatcatt gaaatggatt ggtcagaaat tgctatctgt
     2461 gtaaaatgtc taccagtagc cagatgcttc cagagttctt aatgcctctc tggcatttca
     2521 gagccagcat cccccaactc ccacccctct gccatcaccc aacccaaaca catcagcttt
     2581 caaatgagat gatagtaaat gcggcaatgt taagacaaga aatttatgat ttgccagatt
     2641 caacatttat gacctcccct tccaaagact gtctccgttg accttgtctt tttggtatgc
     2701 cttggggttt ctgataatgt gtggagtctc attatggctg agagtttagt gttttcacag
     2761 tgaagtgcag acatttgatt tctttatgag ttccctgtgt tagaaatggc tatagaaaaa
     2821 tttgtcataa taatttcatt tgcatgaaat cctgaggggt gcattaagga aactaaaagc
     2881 accacttacc aaatctatcg gcagaactga tgtgaggtaa gtgagcatgt caaacaaaat
     2941 aggagctcac atggatatat ttatgtcact gagttgtcag aaattatgtc aaaatgaaaa
     3001 ctgtttgttt catgacaaat tatatagtct ataaattaaa ctggaagtaa ttattacttt
     3061 aattgcagca aaaggagttt gtgagggagc ggtgagaccc aagattggga aagtaggcac
     3121 atgagttcat tcagcaaata tttggttatc tatgtctgtc actgtgctga cactgggaat
     3181 acaaaggtgg ccaaagatca tctagaacaa tggttcccag tggggtgggc agaaagattt
     3241 tgccccccag gagacagctg gcaatgtgtg gagacacttt tggaggtgga gggtggtgag
     3301 gggtactacc agtatcaatg ggtggaggcc agggatgagg ctaaacaccc aaccctcatg
     3361 gattagtttg ccagggctgc cgtcacaaga tactgcagac ggggaagctt acacaacaga
     3421 aatgtgtatt ctcagaattc tggaggctgg aagtccaaga tcagtagggt ttcttcttcg
     3481 gcctctctcc ttggcttcca ctcatggtgt ccttgcatgg tcttttctct gtgtgcacat
     3541 gactgtgcag gactggtgtc ccgatttctt gtagggacag cagtcattgg atcagggccc
     3601 atgcgtatgg cctcatttta cttcagttac ctctttttta gatagagggc cctatctcta
     3661 aatactgtca cattctgaga taccggcagt taagacttcc aacatattta gtggtagggg
     3721 ttaaggggca cgctttagac tacaacactc cacagtaaag aattatccag tctaacatgt
     3781 cagttgtgcc aagactgaga aacctgtaat gtaaaggaac tcccaagtct acctgaaaag
     3841 taaggtctaa agagcattta ttgagcacta tctgttagca cttagcatat gtttaatatt
     3901 catacattga aagaaatgta tgttcatatc catctgacaa agattaagga acttagagta
     3961 agtaatagat ccaaaattga aactgataaa cttctggttc taaaattcac cttttagtga
     4021 aaatacagtt tctcaccaaa cttacagtaa ttcagagtta ttaactcttt ctacctcctc
     4081 actcccctca aagttataac atctcccatc aacatcagct cagaatggca gtgatgctta
     4141 tggtttgtgg gggtgcaggt gagctgctgg atgcacttta gatggcctag tatgccaggc
     4201 cgtcctttca tcctctactc tcgtctttct ttttcgacct catgttgagg attactggtc
     4261 tagttgaaaa aagaattcat acacataaga ccatgtatat aatagaggga ggtatgcctt
     4321 taaaaacaaa attacatggt aaagactgat gtgtcaacag ggatagacaa catagagaag
     4381 gctagaactg tttctggaaa aaaacccaaa aacaatggat ttcaaacttc ttagcatccg
     4441 ataaaagaaa gtagcaatta ttcaaatgag aaacacttct gtctgttatg tacatattat
     4501 gaaagtatga acataagagg gaaaaccaca accattacat tttttaccat ttcagtaatt
     4561 tttttttggt ttttagtctg tttttagaca tccttaagaa caactatctg aggctgtaaa
     4621 caagtattta cattaacacc catgatactg actttatact ccctacctgc attgagacct
     4681 taaagggggc atttctccat tccttctgtg tatttctggg tatccccagg ttcagatatt
     4741 cagaatacag atacaagtcc atgcttgcat atgttgctgc tccccccgac agacttctac
     4801 cagcatctgc cctcctcacc gctgctctgt cacgtgcact gcggctggcc ctgctggaaa
     4861 gtcctacctt gcctaggacg ccatccccga tatagcccca agatgcaaac ctgccaagtg
     4921 ctagggcagc cctgccttcc tgtccatttc agtctcttgg ccttgctgtg ttccaagtgg
     4981 aaatgggagt gtgctttcct ggtttgctag tgtactatca cccggaccac agcgtcctgt
     5041 gcaaaaattt gcttcctctc tcttcccctt tcactctttc tcttttctct tccccacccc
     5101 cttttttttc tctttcccct tacctttctt tcctttccac tcttcctgtc cttccttcct
     5161 tcaactatta ggactatatg tcaagtaatg tgcaaaacaa agacagttcc tgccctgatg
     5221 gagcttgtag atcagaaggg aagatgagca ttaagtaatt atatagatta atgtacaact
     5281 gtgatgagtg caacaaaaga gaggttcatg gcactatcaa acatgcaatg gacggatttt
     5341 gtttttgcct ataaactctt gccatagaaa gcttgaaaat cagtccaggg ggacagcatt
     5401 aatcaccatg ttctttcctt atccctgtct tccccaaaat tcatgtgttg aaacttaaca
     5461 agaggtggga actttaggat gtgattaagt cgtgagggca cagctcttat agatggggtc
     5521 acggtccttc taaaagggct tgagggagtg gttttgtttc cttccatccc ttccgccaca
     5581 tgaggacaca gtcttcgttc cctctggagg actcagcaac aacacaccat cttggaagca
     5641 gagagcagtc ctcaccagac acggaatctg ccagtgcctt gatcttggac ttaccagcct
     5701 ccatacctgt gagaaataaa tttctattgt ctataagtta cccagactgt ggtattttct
     5761 tctggcagca caaatggaca cccagaaact gatgcatgca tcttctgact cctttgattc
     5821 caaaatacaa aatattagaa taaagttatg gaagtca
//
