LOCUS       NM_133338               3054 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens RAD17 checkpoint clamp loader component (RAD17),
            transcript variant 1, mRNA.
ACCESSION   NM_133338
VERSION     NM_133338.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Joris,S., Giron,P., Olsen,C., Seneca,S., Gheldof,A., Staessens,S.,
            Shahi,R.B., De Brakeleer,S., Teugels,E., De Greve,J. and Hes,F.J.
  TITLE     Identification of RAD17 as a candidate cancer predisposition gene
            in families with histories of pancreatic and breast cancers
  JOURNAL   BMC Cancer 24 (1), 723 (2024)
   PUBMED   38872153
  REMARK    GeneRIF: Identification of RAD17 as a candidate cancer
            predisposition gene in families with histories of pancreatic and
            breast cancers.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Fukumoto,Y., Hoshino,T., Nakayama,Y. and Ogra,Y.
  TITLE     The C-terminal tail of Rad17, iVERGE, binds the 9-1-1 complex
            independently of AAA+ ATPase domains to provide another
            clamp-loader interface
  JOURNAL   DNA Repair (Amst) 130, 103567 (2023)
   PUBMED   37713925
  REMARK    GeneRIF: The C-terminal tail of Rad17, iVERGE, binds the 9-1-1
            complex independently of AAA+ ATPase domains to provide another
            clamp-loader interface.
REFERENCE   3  (bases 1 to 3054)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   4  (bases 1 to 3054)
  AUTHORS   Sun,J., Lin,W., Wang,Q., Sakai,A., Xue,R., Watanabe,M., Liu,C.,
            Sadahira,T., Nasu,Y., Xu,A. and Huang,P.
  TITLE     The Cell Cycle Checkpoint Gene, RAD17 rs1045051, Is Associated with
            Prostate Cancer Risk
  JOURNAL   Acta Med Okayama 75 (4), 415-421 (2021)
   PUBMED   34511607
  REMARK    GeneRIF: The Cell Cycle Checkpoint Gene, RAD17 rs1045051, Is
            Associated with Prostate Cancer Risk.
REFERENCE   5  (bases 1 to 3054)
  AUTHORS   Fukumoto,Y., Ikeuchi,M., Qu,L., Hoshino,T., Yamaguchi,N.,
            Nakayama,Y. and Ogra,Y.
  TITLE     Nuclear translocation promotes proteasomal degradation of human
            Rad17 protein through the N-terminal destruction boxes
  JOURNAL   J Biol Chem 297 (2), 100831 (2021)
   PUBMED   34174284
  REMARK    GeneRIF: Nuclear translocation promotes proteasomal degradation of
            human Rad17 protein through the N-terminal destruction boxes.
REFERENCE   6  (bases 1 to 3054)
  AUTHORS   Bluyssen,H.A., Naus,N.C., van Os,R.I., Jaspers,I., Hoeijmakers,J.H.
            and de Klein,A.
  TITLE     Human and mouse homologs of the Schizosaccharomyces pombe rad17+
            cell cycle checkpoint control gene
  JOURNAL   Genomics 55 (2), 219-228 (1999)
   PUBMED   9933569
REFERENCE   7  (bases 1 to 3054)
  AUTHORS   Dean,F.B., Lian,L. and O'Donnell,M.
  TITLE     cDNA cloning and gene mapping of human homologs for
            Schizosaccharomyces pombe rad17, rad1, and hus1 and cloning of
            homologs from mouse, Caenorhabditis elegans, and Drosophila
            melanogaster
  JOURNAL   Genomics 54 (3), 424-436 (1998)
   PUBMED   9878245
REFERENCE   8  (bases 1 to 3054)
  AUTHORS   Freire,R., Murguia,J.R., Tarsounas,M., Lowndes,N.F., Moens,P.B. and
            Jackson,S.P.
  TITLE     Human and mouse homologs of Schizosaccharomyces pombe rad1(+) and
            Saccharomyces cerevisiae RAD17: linkage to checkpoint control and
            mammalian meiosis
  JOURNAL   Genes Dev 12 (16), 2560-2573 (1998)
   PUBMED   9716408
REFERENCE   9  (bases 1 to 3054)
  AUTHORS   Parker,A.E., Van de Weyer,I., Laus,M.C., Verhasselt,P. and
            Luyten,W.H.
  TITLE     Identification of a human homologue of the Schizosaccharomyces
            pombe rad17+ checkpoint gene
  JOURNAL   J Biol Chem 273 (29), 18340-18346 (1998)
   PUBMED   9660800
  REMARK    Erratum:[J Biol Chem 1999 Aug 20;274(34):24438]
REFERENCE   10 (bases 1 to 3054)
  AUTHORS   al-Khodairy,F. and Carr,A.M.
  TITLE     DNA repair mutants defining G2 checkpoint pathways in
            Schizosaccharomyces pombe
  JOURNAL   EMBO J 11 (4), 1343-1350 (1992)
   PUBMED   1563350
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK292487.1, AF017748.1 and
            AF126424.1.
            
            On Nov 22, 2018 this sequence version replaced NM_133338.2.
            
            Summary: The protein encoded by this gene is highly similar to the
            gene product of Schizosaccharomyces pombe rad17, a cell cycle
            checkpoint gene required for cell cycle arrest and DNA damage
            repair in response to DNA damage. This protein shares strong
            similarity with DNA replication factor C (RFC), and can form a
            complex with RFCs. This protein binds to chromatin prior to DNA
            damage and is phosphorylated by the checkpoint kinase ATR following
            damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein
            complex onto chromatin after DNA damage, which may be required for
            its phosphorylation. The phosphorylation of this protein is
            required for the DNA-damage-induced cell cycle G2 arrest, and is
            thought to be a critical early event during checkpoint signaling in
            DNA-damaged cells. Multiple alternatively spliced transcript
            variants of this gene, which encode four distinct protein isoforms,
            have been reported. Two pseudogenes, located on chromosomes 7 and
            13, have been identified. [provided by RefSeq, Jul 2013].
            
            Transcript Variant: This variant (1) encodes the predominant
            isoform (1). Variants 1, 5, 6, 7, 8 and 9 encode the same isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF126424.1, SRR1660809.72334.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968832
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000354868.10/ ENSP00000346938.5
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-91                AK292487.1         88-178
            92-116              AF017748.1         68-92
            117-3054            AF126424.1         122-3059
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q13.2"
     gene            1..3054
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /note="RAD17 checkpoint clamp loader component"
                     /db_xref="GeneID:5884"
                     /db_xref="HGNC:HGNC:9807"
                     /db_xref="MIM:603139"
     exon            1..116
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /inference="alignment:Splign:2.1.0"
     exon            117..253
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /inference="alignment:Splign:2.1.0"
     exon            254..357
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /inference="alignment:Splign:2.1.0"
     exon            358..541
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /inference="alignment:Splign:2.1.0"
     CDS             533..2545
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /note="isoform 1 is encoded by transcript variant 1; RAD1
                     homolog; cell cycle checkpoint protein (RAD17); Rad17-like
                     protein; RF-C activator 1 homolog; RAD17 homolog"
                     /codon_start=1
                     /product="cell cycle checkpoint protein RAD17 isoform 1"
                     /protein_id="NP_579916.1"
                     /db_xref="CCDS:CCDS4004.1"
                     /db_xref="GeneID:5884"
                     /db_xref="HGNC:HGNC:9807"
                     /db_xref="MIM:603139"
                     /translation="MNQVTDWVDPSFDDFLECSGVSTITATSLGVNNSSHRRKNGPST
                     LESSRFPARKRGNLSSLEQIYGLENSKEYLSENEPWVDKYKPETQHELAVHKKKIEEV
                     ETWLKAQVLERQPKQGGSILLITGPPGCGKTTTLKILSKEHGIQVQEWINPVLPDFQK
                     DDFKGMFNTESSFHMFPYQSQIAVFKEFLLRATKYNKLQMLGDDLRTDKKIILVEDLP
                     NQFYRDSHTLHEVLRKYVRIGRCPLIFIISDSLSGDNNQRLLFPKEIQEECSISNISF
                     NPVAPTIMMKFLNRIVTIEANKNGGKITVPDKTSLELLCQGCSGDIRSAINSLQFSSS
                     KGENNLRPRKKGMSLKSDAVLSKSKRRKKPDRVFENQEVQAIGGKDVSLFLFRALGKI
                     LYCKRASLTELDSPRLPSHLSEYERDTLLVEPEEVVEMSHMPGDLFNLYLHQNYIDFF
                     MEIDDIVRASEFLSFADILSGDWNTRSLLREYSTSIATRGVMHSNKARGYAHCQGGGS
                     SFRPLHKPQWFLINKKYRENCLAAKALFPDFCLPALCLQTQLLPYLALLTIPMRNQAQ
                     ISFIQDIGRLPLKRHFGRLKMEALTDREHGMIDPDSGDEAQLNGGHSAEESLGEPTQA
                     TVPETWSLPLSQNSASELPASQPQPFSAQGDMEENIIIEDYESDGT"
     exon            542..799
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /inference="alignment:Splign:2.1.0"
     exon            800..883
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /inference="alignment:Splign:2.1.0"
     exon            884..1040
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1177
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /inference="alignment:Splign:2.1.0"
     exon            1178..1230
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /inference="alignment:Splign:2.1.0"
     exon            1231..1356
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..1426
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /inference="alignment:Splign:2.1.0"
     exon            1427..1538
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /inference="alignment:Splign:2.1.0"
     exon            1539..1721
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1807
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /inference="alignment:Splign:2.1.0"
     exon            1808..1954
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /inference="alignment:Splign:2.1.0"
     exon            1955..2104
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /inference="alignment:Splign:2.1.0"
     exon            2105..2225
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /inference="alignment:Splign:2.1.0"
     exon            2226..2283
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /inference="alignment:Splign:2.1.0"
     exon            2284..3054
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3036..3041
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /note="hexamer: ATTAAA"
     polyA_site      3054
                     /gene="RAD17"
                     /gene_synonym="CCYC; HRAD17; R24L; RAD17SP; RAD24"
                     /note="major polyA site"
ORIGIN      
        1 gtacctccga gaggctcggc gttgagcccg ggtagggcca ggtggctgcc ctttcaccta
       61 gggtagtccc tggtcgcctc cgctcttcgc ctaaaagggg atgcagctcc gggaaacaag
      121 tgaattcatg gtattttact tttttgggaa atactggaaa tgaagacctg caactgtaat
      181 ttgaaataag gaaaacttta attttcagta taaaaattgc tcaaatagaa ttgcctgatt
      241 ttaatgacaa aaggtgaatt atagtttaat gtactgcaag tcctaaacta cggatgggaa
      301 ctattacagt ttataatgtc aaaaactttt cttagaccaa aggtatcttc cacaaagtat
      361 atgggagtcc acatttatgt aagaaatgaa actataaaat gtataaataa tttgcaaatc
      421 agaattgctg tcgaaagttt tactataatg aaagatattt tcatactctc aaaaatatag
      481 aggaaagggg ccaagattat agtaccagtc acaatctttt gatgaggacg aaatgaatca
      541 ggtaacagac tgggttgacc catcatttga tgattttcta gagtgtagtg gcgtctctac
      601 tattactgcc acatcattag gtgtgaataa ctcaagtcat agaagaaaaa atgggccttc
      661 tacattagaa agcagcagat ttccagcgag aaaaagagga aatctatctt ccttagaaca
      721 gatttatggt ttagaaaatt caaaagaata tctgtctgaa aatgaaccat gggtggataa
      781 atataaacca gaaactcagc atgaacttgc tgtgcataaa aagaaaattg aagaagtcga
      841 aacctggtta aaagctcaag ttttagaaag gcaaccaaaa cagggtggat ctattttatt
      901 aataacaggt cctcctggat gtggaaagac aacgacctta aaaatactat caaaggagca
      961 tggtattcaa gtacaagagt ggattaatcc agttttacca gacttccaaa aagatgattt
     1021 caaggggatg tttaatactg aatcaagctt ccatatgttt ccctatcagt ctcagatagc
     1081 agttttcaaa gagtttctac taagagcgac aaagtataac aagttacaaa tgcttggaga
     1141 tgatctgaga actgataaga agataattct ggttgaagat ttacctaacc agttttatcg
     1201 ggattctcat actttacatg aagttctaag gaagtatgtg aggattggtc gatgtcctct
     1261 tatatttata atctcggaca gtctcagtgg agataataat caaaggttat tgtttcccaa
     1321 agaaattcag gaagagtgtt ctatctcaaa tattagtttc aaccctgtgg caccaacaat
     1381 tatgatgaaa tttcttaatc gaatagtgac tatagaagct aacaagaatg gaggaaaaat
     1441 tactgtccct gacaaaactt ctctagagtt gctctgtcag ggatgttctg gtgatatcag
     1501 aagtgcaata aacagcctcc agttttcttc ttcaaaagga gaaaacaact tacggccaag
     1561 gaaaaaagga atgtctttaa aatcagatgc tgtgctgtca aaatcaaaac gaagaaaaaa
     1621 acctgatagg gtttttgaaa atcaagaggt ccaagctatt ggtggcaaag atgtttctct
     1681 gtttctcttc agagctttgg ggaaaattct atattgtaaa agagcatctt taacagaatt
     1741 agactcacct cggttgccct ctcatttatc agaatatgaa cgggatacat tacttgttga
     1801 acctgaggag gtagtagaaa tgtcacacat gcctggagac ttatttaatt tatatcttca
     1861 ccaaaactac atagatttct tcatggaaat tgatgatatt gtgagagcca gtgaatttct
     1921 gagttttgca gatatcctca gtggtgactg gaatacacgc tctttactca gggaatatag
     1981 cacatctata gctacgagag gtgtgatgca ttccaacaaa gcccgaggat atgctcattg
     2041 ccaaggagga ggatcaagtt ttcgaccctt gcacaaacct cagtggtttc taataaataa
     2101 aaagtatcgg gaaaattgcc tggcagcaaa agcacttttt cctgacttct gcctaccagc
     2161 tttatgcctc caaactcagc tattgccata ccttgctcta ctaaccattc caatgagaaa
     2221 tcaagctcag atttctttta tccaagatat tggaaggctc cctctgaagc gacactttgg
     2281 aagattgaaa atggaagccc tgactgacag ggaacatgga atgatagacc ctgacagcgg
     2341 agatgaagcc cagcttaatg gaggacattc tgcagaggaa tctctgggtg aacccactca
     2401 agccactgtg ccggaaacct ggtctcttcc tttgagtcag aatagtgcca gtgaactgcc
     2461 tgctagccag ccccagccct tttcagccca aggagacatg gaagaaaaca taataataga
     2521 agactacgag agtgatggga catagaagcc agcctgctaa tcagattgct acttcacagc
     2581 ttcatttttg tttcattcag tggtacttca gcagagttaa tatgcttttc tgatgaatta
     2641 cacaacagtt tgttaattct tcattcttgt agtatttcat cacaagaaac ctactcttct
     2701 gtcatcttga agtaaataga agatcaagcc ttcaaatctc ttaatttttt cggtatttat
     2761 taaatctgtg agtggtttaa ggagcggtca gtgtgtataa agtgtgtttg aacattatgc
     2821 caaatatcaa gatgtgaagg actaattcag gatgcaaaaa cgttattggg gggttgtaaa
     2881 tatcaactat tcaacagttt aggatgcaat tacgagtgta aactgtgtgc cttatttaca
     2941 ctttattgtc tcccgcttct cagatagttt tgatgtgttg tacagtggaa tatcttagat
     3001 actttttgga aagtatttac ataagttata tcacaattaa aatgttgaat ttaa
//
