LOCUS       NM_002853               4512 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens RAD1 checkpoint DNA exonuclease (RAD1), transcript
            variant 1, mRNA.
ACCESSION   NM_002853
VERSION     NM_002853.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Hara,K., Hishiki,A., Hoshino,T., Nagata,K., Iida,N., Sawada,Y.,
            Ohashi,E. and Hashimoto,H.
  TITLE     The 9-1-1 DNA clamp subunit RAD1 forms specific interactions with
            clamp loader RAD17, revealing functional implications for
            binding-protein RHINO
  JOURNAL   J Biol Chem 299 (4), 103061 (2023)
   PUBMED   36841485
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 4512)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 4512)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 4512)
  AUTHORS   Hara,K., Iida,N., Tamafune,R., Ohashi,E., Sakurai,H., Ishikawa,Y.,
            Hishiki,A. and Hashimoto,H.
  TITLE     Structure of the RAD9-RAD1-HUS1 checkpoint clamp bound to RHINO
            sheds light on the other side of the DNA clamp
  JOURNAL   J Biol Chem 295 (4), 899-904 (2020)
   PUBMED   31776186
REFERENCE   6  (bases 1 to 4512)
  AUTHORS   Bluyssen,H.A., van Os,R.I., Naus,N.C., Jaspers,I., Hoeijmakers,J.H.
            and de Klein,A.
  TITLE     A human and mouse homolog of the Schizosaccharomyces pombe rad1+
            cell cycle checkpoint control gene
  JOURNAL   Genomics 54 (2), 331-337 (1998)
   PUBMED   9828137
REFERENCE   7  (bases 1 to 4512)
  AUTHORS   Udell,C.M., Lee,S.K. and Davey,S.
  TITLE     HRAD1 and MRAD1 encode mammalian homologues of the fission yeast
            rad1(+) cell cycle checkpoint control gene
  JOURNAL   Nucleic Acids Res 26 (17), 3971-3976 (1998)
   PUBMED   9705507
REFERENCE   8  (bases 1 to 4512)
  AUTHORS   Freire,R., Murguia,J.R., Tarsounas,M., Lowndes,N.F., Moens,P.B. and
            Jackson,S.P.
  TITLE     Human and mouse homologs of Schizosaccharomyces pombe rad1(+) and
            Saccharomyces cerevisiae RAD17: linkage to checkpoint control and
            mammalian meiosis
  JOURNAL   Genes Dev 12 (16), 2560-2573 (1998)
   PUBMED   9716408
REFERENCE   9  (bases 1 to 4512)
  AUTHORS   Parker,A.E., Van de Weyer,I., Laus,M.C., Oostveen,I., Yon,J.,
            Verhasselt,P. and Luyten,W.H.
  TITLE     A human homologue of the Schizosaccharomyces pombe rad1+ checkpoint
            gene encodes an exonuclease
  JOURNAL   J Biol Chem 273 (29), 18332-18339 (1998)
   PUBMED   9660799
REFERENCE   10 (bases 1 to 4512)
  AUTHORS   Bardwell,A.J., Bardwell,L., Johnson,D.K. and Friedberg,E.C.
  TITLE     Yeast DNA recombination and repair proteins Rad1 and Rad10
            constitute a complex in vivo mediated by localized hydrophobic
            domains
  JOURNAL   Mol Microbiol 8 (6), 1177-1188 (1993)
   PUBMED   8361362
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF011905.1 and AC026801.7.
            
            On Nov 23, 2018 this sequence version replaced NM_002853.3.
            
            Summary: This gene encodes a component of a heterotrimeric cell
            cycle checkpoint complex, known as the 9-1-1 complex, that is
            activated to stop cell cycle progression in response to DNA damage
            or incomplete DNA replication. The 9-1-1 complex is recruited by
            RAD17 to affected sites where it may attract specialized DNA
            polymerases and other DNA repair effectors. Alternatively spliced
            transcript variants of this gene have been described. [provided by
            RefSeq, Jan 2009].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the functional protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The extent of this transcript is
            supported by transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803611.208746.1,
                                           SRR1660809.101247.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2142680, SAMEA2159080
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000382038.7/ ENSP00000371469.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1222              AF011905.1         57-1278
            1223-4512           AC026801.7         147398-150687       c
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p13.2"
     gene            1..4512
                     /gene="RAD1"
                     /gene_synonym="HRAD1; REC1"
                     /note="RAD1 checkpoint DNA exonuclease"
                     /db_xref="GeneID:5810"
                     /db_xref="HGNC:HGNC:9806"
                     /db_xref="MIM:603153"
     exon            1..89
                     /gene="RAD1"
                     /gene_synonym="HRAD1; REC1"
                     /inference="alignment:Splign:2.1.0"
     exon            90..356
                     /gene="RAD1"
                     /gene_synonym="HRAD1; REC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             159..1007
                     /gene="RAD1"
                     /gene_synonym="HRAD1; REC1"
                     /EC_number="3.1.11.2"
                     /note="exonuclease homolog RAD1; cell cycle checkpoint
                     protein Hrad1; DNA repair exonuclease REC1; checkpoint
                     control protein HRAD1; DNA repair exonuclease rad1
                     homolog; RAD1 homolog; RAD1 checkpoint clamp component;
                     rad1-like DNA damage checkpoint protein"
                     /codon_start=1
                     /product="cell cycle checkpoint protein RAD1"
                     /protein_id="NP_002844.1"
                     /db_xref="CCDS:CCDS3905.1"
                     /db_xref="GeneID:5810"
                     /db_xref="HGNC:HGNC:9806"
                     /db_xref="MIM:603153"
                     /translation="MPLLTQQIQDEDDQYSLVASLDNVRNLSTILKAIHFREHATCFA
                     TKNGIKVTVENAKCVQANAFIQAGIFQEFKVQEESVTFRINLTVLLDCLSIFGSSPMP
                     GTLTALRMCYQGYGYPLMLFLEEGGVVTVCKINTQEPEETLDFDFCSTNVINKIILQS
                     EGLREAFSELDMTSEVLQITMSPDKPYFRLSTFGNAGSSHLDYPKDSDLMEAFHCNQT
                     QVNRYKISLLKPSTKALVLSCKVSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEE
                     VPESES"
     exon            357..465
                     /gene="RAD1"
                     /gene_synonym="HRAD1; REC1"
                     /inference="alignment:Splign:2.1.0"
     exon            466..724
                     /gene="RAD1"
                     /gene_synonym="HRAD1; REC1"
                     /inference="alignment:Splign:2.1.0"
     exon            725..823
                     /gene="RAD1"
                     /gene_synonym="HRAD1; REC1"
                     /inference="alignment:Splign:2.1.0"
     exon            824..4512
                     /gene="RAD1"
                     /gene_synonym="HRAD1; REC1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1195..1200
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD1"
                     /gene_synonym="HRAD1; REC1"
                     /note="hexamer: AATAAA"
     polyA_site      1223
                     /gene="RAD1"
                     /gene_synonym="HRAD1; REC1"
                     /note="major polyA site"
     regulatory      2484..2489
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD1"
                     /gene_synonym="HRAD1; REC1"
                     /note="hexamer: AATAAA"
     polyA_site      2503
                     /gene="RAD1"
                     /gene_synonym="HRAD1; REC1"
                     /note="major polyA site"
     regulatory      4487..4492
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAD1"
                     /gene_synonym="HRAD1; REC1"
                     /note="hexamer: AATAAA"
     polyA_site      4512
                     /gene="RAD1"
                     /gene_synonym="HRAD1; REC1"
                     /note="major polyA site"
ORIGIN      
        1 acttcctccg cggttcctcg gagccgcctc gctcctcttc agggactttg ctgagaaggg
       61 ctctcgggcg tccagacccc accgcaaagg tgtttggcga tccgccgaga agttgttggc
      121 cccaggagca tccctcgggg ccgaatgcgc agtggacgat gccccttctg acccaacaga
      181 tccaagacga ggatgatcag tacagccttg tggccagcct tgacaacgtt aggaatctct
      241 ccactatctt gaaagctatt catttccgag aacatgccac gtgtttcgca actaaaaatg
      301 gtatcaaagt aacagtggaa aatgcaaagt gtgtgcaagc aaatgctttt attcaggctg
      361 gaatatttca ggagtttaaa gttcaggaag agtctgttac ttttcgaatt aatttaactg
      421 tccttttaga ctgtttatct atttttggat caagtcctat gccagggact ttaactgcac
      481 ttcgaatgtg ttaccaaggt tatggttacc ctttgatgct gttcctggaa gaaggaggag
      541 tggtgacagt ctgcaaaatc aatacacagg aacctgagga gaccctggac tttgatttct
      601 gcagcaccaa tgttattaat aaaattattc tgcagtcaga ggggctccgt gaagcatttt
      661 ctgaattgga tatgacgagt gaagtcctac aaattaccat gtctcctgac aagccttatt
      721 tcaggttatc tacttttgga aatgcaggaa gttcccacct tgactatccc aaagattctg
      781 atttgatgga agcatttcat tgtaatcaga cccaagtcaa cagatacaag atttccttac
      841 tgaaaccctc tacaaaggca ttagtcctat cttgtaaggt atctattcgg acagataaca
      901 gaggcttcct ttcattacag tatatgatta gaaatgaaga tggacaaata tgttttgtgg
      961 aatattactg ctgccctgat gaagaagttc ctgaatctga gtcttgagta tgacaattca
     1021 ctgatattta tgtgtacatt tatgatagat gaagttctta ttctgagtac agtactcttt
     1081 gtcatttcat attggatttt ctatagagaa gaagcacaat ggggaagata ggagcaaggt
     1141 catgtaccct aatagttact atgttttgta aatccatttt gtagagggca tgtaaataaa
     1201 tgttttcctg tagtcataga ttattcagga ctgtccttta gttctgtctt ttgaactcat
     1261 gggaataatt gtgagtcagc gtaacatttc aagagtctaa aggtggccgg gtgtggtggc
     1321 tttaatccca gcactttggg aagccgaggt gggcggatca cctgaggtca ggagttcgag
     1381 accagcctga ccaacgtgga gaaaccccat ctctactaaa aatacaaaaa attagccgga
     1441 cgtggtggca catgcctgta atgccagcta ctcgggaggc tgaggcagga gaattgcttg
     1501 aacccgggag gcggaggttg tggtgagctg agatgacgcc attgcactcc agcctaggca
     1561 ataagagcaa aactctgcct caaaaaaaaa aaaaaaaaaa gtctaaaggc ttaaagtttg
     1621 atgcagctac ctgaaatgat cttttattta tttattatta gaaaaagcaa aggcatatgg
     1681 gcattgctta ttagtttgaa ttctagagac tagatcttaa agtagtggtt ctcaaagtgt
     1741 tgtgcccgca ccaacatcag aatggcctgc aaacttgtag caaactctgg ggaggaggcc
     1801 agcattctgt attttaacaa gcttccctca ggagattctg atgcctgcta aattttggga
     1861 accactgttt taaaggaaac tttttttttc tttaatagca tttaattgta tgagatgatt
     1921 gcttttacat gtgatttcct tgcaaatgtt ctgaagttga ggcatcacca aacaagtctg
     1981 aacaattctt tatgtgattt atttttaaag tagacctttt gaagagatct atgaatggga
     2041 tataaagcaa ttttcagtgt tacaggtttt cttcttcttc tcaaaactgt ttgctgtaag
     2101 taactgcaat cagtacttac tactttccat ttgcttatga gtttcttgac aaatcaaggt
     2161 gtagaaaacc agttattaag tgattttgta ctttcctggt agttgtcact aaaataattt
     2221 ttgtggcata taaatatatt taataaaatg caaaaattat cttcctgtct agtagaaaaa
     2281 attacatgag taaagtgaag cttctgtctt tgttactgta ccaggtgaca acagctgagt
     2341 gtccctccat ggacagtcac tattggcctt ttgagtgaga cagttcttta ggataaaaac
     2401 ctgtcatccc attgcaggat tcatttagcc tttctggccc ttacccactg atgctagtca
     2461 ttgtgaccac cccacctccc ccaaataaaa gtgtgccaaa ctattcattg ttgagaacca
     2521 ttcaattgta ttcaccaaaa ttggaggata tattggaatg ccctacctta atataggtca
     2581 catagcatta gagaaaattg gtttggagag tattacagca aagtgtgctg caaggaggct
     2641 actccagcac aatggcaggc actgcttccc aggcatatgg acaattctgg gaggtggcca
     2701 gggtcagcac ctgataacta gtaagtgtct caatctcagc atacaaaatt tagcagtgac
     2761 tacaaaagaa gttggaattt tctaaaatgt ttgtattcaa gaaggaaaca aatgaattta
     2821 ctaaatgttg tcagagtatt ttttgtttag ataaccacca agaacagaac aaacctaaaa
     2881 gatacttggg aaaatatcta atcagtcaac attctaagga ctgttaaatg tgaaatagca
     2941 tagttatttt ttgtcttttg ttactagtgt gaacaaagaa cggcgcctaa catataatgg
     3001 gcatttagta actctgttag atgaaagact ctccaatttc agattttctt ttagcatctt
     3061 attagtcact acttaattct gatgatttag ctctaaattt attttgatct cagttttttt
     3121 atttttattt ttggagacaa gatcttgctc tgttgcccag gctggagtac agttgtagga
     3181 ttacagttca ctccagcctc aatctcctag gctcaagcaa tcctcccatc tcagcttccc
     3241 aaatagctac gactacaggt gcatgcctcc atgccctgct aatttttgta ttttttgtag
     3301 agatggggtt ttactatgtt ccctaggcta atcttgaact cctgagctca agcaatccac
     3361 ccgcctcggc ctcccaaagg gctgggatta caggcgtgag ctatcatgcc tggcctaatg
     3421 tattttttaa aagttgaatc taaaaagaat ttaaaacaaa tataaatcag ctcagtaagg
     3481 atggatattt ttgtacattt aaaactatta ttttaggccg ggagtggtgg ctcacacctg
     3541 taatcccagc attttgggaa gccgaggcag ggggatcatc tgaggtcagg aagttcgaga
     3601 tcagcctggc caacatggtg aaacccctac ctctactaaa aatacaaaaa ttagctgggt
     3661 gtggtggtgg gtgtctgtaa tcccagctac ttgagaggct gaggcaagag aatcacttga
     3721 aactggaagg cgggggttgc agtgagccga gattgcacca ttgcactcca gcctgggtga
     3781 cagagcaaga ctccgtctca aaaacaaaaa aaattaaaat aaaaattatt gttttagctg
     3841 catgtcttca gctatttaga attcaaagac taatgaattg tttctaagtc accctaacat
     3901 caaggtactt attttataaa gaatttcctc cttatttgtg ttctaattga cccaggaatt
     3961 tttactttaa taaaagttta actttatttc ctatggaaaa tcactagggg gagtgctccc
     4021 tcaggcagag caggcgacac tttggtgcca ctacaaattg atgtgtagta tatagaagaa
     4081 tacagtctta tatttaaaga cttttagttc tgtctttttg aagtttatct ctctttctag
     4141 taaaatggtg cttactgtga atggataatc ttttttttca tttttcattt gttgatttcc
     4201 cattcgatga tgtagtgctt ccaccgagaa ttgatgaaat gcttcaaaat aataaaagta
     4261 tttgttaaat tccactttaa taaaaataag gcatgtaaca cagtcatttt aacaggaagc
     4321 cacagggttt ctgtggtgtt accaaagtcc tagtaaaaat taattaaaat tgctaaaagg
     4381 gcagaatgtg gcactctcaa cagtggggta ggcagtcctt cagaacatag agacttcctt
     4441 ctttccttcc ctgtcttcac attgcttgtc ccttatcccc acctgaaata aaatctattg
     4501 ggtcctgaga aa
//
