LOCUS       NM_005370               2567 bp    mRNA    linear   PRI 08-OCT-2024
DEFINITION  Homo sapiens RAB8A, member RAS oncogene family (RAB8A), mRNA.
ACCESSION   NM_005370
VERSION     NM_005370.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Alexander,K.K., Naaldijk,Y., Fasiczka,R., Brahmia,B., Chen,T.,
            Hilfiker,S. and Kennedy,E.J.
  TITLE     Targeting Rab-RILPL interactions as a strategy to downregulate
            pathogenic LRRK2 in Parkinson's disease
  JOURNAL   J Pept Sci 30 (5), e3563 (2024)
   PUBMED   38135900
  REMARK    GeneRIF: Targeting Rab-RILPL interactions as a strategy to
            downregulate pathogenic LRRK2 in Parkinson's disease.
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Mamais,A., Sanyal,A., Fajfer,A., Zykoski,C.G., Guldin,M.,
            Riley-DiPaolo,A., Subrahmanian,N., Gibbs,W., Lin,S. and LaVoie,M.J.
  TITLE     The LRRK2 kinase substrates RAB8a and RAB10 contribute
            complementary but distinct disease-relevant phenotypes in human
            neurons
  JOURNAL   Stem Cell Reports 19 (2), 163-173 (2024)
   PUBMED   38307024
  REMARK    GeneRIF: The LRRK2 kinase substrates RAB8a and RAB10 contribute
            complementary but distinct disease-relevant phenotypes in human
            neurons.
REFERENCE   3  (bases 1 to 2567)
  AUTHORS   Wu,T., Zhang,Y., Han,Q., Lu,X., Cheng,Y., Chen,J., Sha,J. and
            Xia,W.
  TITLE     Klotho-beta attenuates Rab8a-mediated exosome regulation and
            promotes prostate cancer progression
  JOURNAL   Oncogene 42 (38), 2801-2815 (2023)
   PUBMED   37582861
  REMARK    GeneRIF: Klotho-beta attenuates Rab8a-mediated exosome regulation
            and promotes prostate cancer progression.
REFERENCE   4  (bases 1 to 2567)
  AUTHORS   Mahajan,D., Madugula,V. and Lu,L.
  TITLE     Rab8 and TNPO1 are ciliary transport adaptors for GTPase Arl13b by
            interacting with its RVEP motif containing ciliary targeting
            sequence
  JOURNAL   J Biol Chem 299 (5), 104604 (2023)
   PUBMED   36907439
  REMARK    GeneRIF: Rab8 and TNPO1 are ciliary transport adaptors for GTPase
            Arl13b by interacting with its RVEP motif containing ciliary
            targeting sequence.
REFERENCE   5  (bases 1 to 2567)
  AUTHORS   Cai,S., Liu,X., Gao,W., Ye,L., Zhong,Q. and Zhang,X.
  TITLE     Transcription Activation of Rab8A by PEA3 Augments Progression of
            Esophagus Cancer by Activating the Wnt/beta-Catenin Signaling
            Pathway
  JOURNAL   Dis Markers 2023, 8143581 (2023)
   PUBMED   36815135
  REMARK    GeneRIF: Transcription Activation of Rab8A by PEA3 Augments
            Progression of Esophagus Cancer by Activating the Wnt/beta-Catenin
            Signaling Pathway.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2567)
  AUTHORS   Zahraoui,A., Joberty,G., Arpin,M., Fontaine,J.J., Hellio,R.,
            Tavitian,A. and Louvard,D.
  TITLE     A small rab GTPase is distributed in cytoplasmic vesicles in non
            polarized cells but colocalizes with the tight junction marker ZO-1
            in polarized epithelial cells
  JOURNAL   J Cell Biol 124 (1-2), 101-115 (1994)
   PUBMED   8294494
REFERENCE   7  (bases 1 to 2567)
  AUTHORS   Joberty,G., Tavitian,A. and Zahraoui,A.
  TITLE     Isoprenylation of Rab proteins possessing a C-terminal CaaX motif
  JOURNAL   FEBS Lett 330 (3), 323-328 (1993)
   PUBMED   8375503
REFERENCE   8  (bases 1 to 2567)
  AUTHORS   Nimmo,E.R., Sanders,P.G., Padua,R.A., Hughes,D., Williamson,R. and
            Johnson,K.J.
  TITLE     The MEL gene: a new member of the RAB/YPT class of RAS-related
            genes
  JOURNAL   Oncogene 6 (8), 1347-1351 (1991)
   PUBMED   1886711
REFERENCE   9  (bases 1 to 2567)
  AUTHORS   Nimmo,E., Padua,R.A., Hughes,D., Brook,J.D., Williamson,R. and
            Johnson,K.J.
  TITLE     Confirmation and refinement of the localisation of the c-MEL locus
            on chromosome 19 by physical and genetic mapping
  JOURNAL   Hum Genet 81 (4), 382-384 (1989)
   PUBMED   2564840
REFERENCE   10 (bases 1 to 2567)
  AUTHORS   Newport,J. and Roemer,M.I.
  TITLE     Comparative perinatal mortality under medical care foundations and
            other delivery models
  JOURNAL   Inquiry 12 (1), 10-17 (1975)
   PUBMED   123217
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK025165.1, BC002977.1, BU940750.1 and AC008894.9.
            
            On Nov 22, 2018 this sequence version replaced NM_005370.4.
            
            Summary: The protein encoded by this gene is a member of the RAS
            superfamily which are small GTP/GDP-binding proteins with an
            average size of 200 amino acids. The RAS-related proteins of the
            RAB/YPT family may play a role in the transport of proteins from
            the endoplasmic reticulum to the Golgi and the plasma membrane.
            This protein shares 97%, 96%, and 51% similarity with the dog RAB8,
            mouse MEL, and mouse YPT1 proteins, respectively and contains the 4
            GTP/GDP-binding sites that are present in all the RAS proteins. The
            putative effector-binding site of this protein is similar to that
            of the RAB/YPT proteins. However, this protein contains a
            C-terminal CAAX motif that is characteristic of many RAS
            superfamily members but which is not found in YPT1 and the majority
            of RAB proteins. Although this gene was isolated as a transforming
            gene from a melanoma cell line, no linkage between MEL and
            malignant melanoma has been demonstrable. This oncogene is located
            800 kb distal to MY09B on chromosome 19p13.1. [provided by RefSeq,
            Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.28557.1, AK025165.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000300935.8/ ENSP00000300935.2
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1352              AK025165.1         233-1584
            1353-1818           BC002977.1         1416-1881
            1819-1888           BU940750.1         446-515
            1889-1968           BC002977.1         1952-2031
            1969-2567           AC008894.9         92903-93501
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.11"
     gene            1..2567
                     /gene="RAB8A"
                     /gene_synonym="MEL; RAB8"
                     /note="RAB8A, member RAS oncogene family"
                     /db_xref="GeneID:4218"
                     /db_xref="HGNC:HGNC:7007"
                     /db_xref="MIM:165040"
     exon            1..137
                     /gene="RAB8A"
                     /gene_synonym="MEL; RAB8"
                     /inference="alignment:Splign:2.1.0"
     CDS             14..637
                     /gene="RAB8A"
                     /gene_synonym="MEL; RAB8"
                     /EC_number="3.6.5.2"
                     /note="ras-associated protein RAB8; mel transforming
                     oncogene (derived from cell line NK14); mel transforming
                     oncogene (RAB8 homolog); mel transforming oncogene
                     (derived from cell line NK14)- RAB8 homolog; oncogene
                     c-mel"
                     /codon_start=1
                     /product="ras-related protein Rab-8A"
                     /protein_id="NP_005361.2"
                     /db_xref="CCDS:CCDS12339.1"
                     /db_xref="GeneID:4218"
                     /db_xref="HGNC:HGNC:7007"
                     /db_xref="MIM:165040"
                     /translation="MAKTYDYLFKLLLIGDSGVGKTCVLFRFSEDAFNSTFISTIGID
                     FKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIRNW
                     IRNIEEHASADVEKMILGNKCDVNDKRQVSKERGEKLALDYGIKFMETSAKANINVEN
                     AFFTLARDIKAKMDKKLEGNSPQGSNQGVKITPDQQKRSSFFRCVLL"
     exon            138..198
                     /gene="RAB8A"
                     /gene_synonym="MEL; RAB8"
                     /inference="alignment:Splign:2.1.0"
     exon            199..259
                     /gene="RAB8A"
                     /gene_synonym="MEL; RAB8"
                     /inference="alignment:Splign:2.1.0"
     exon            260..337
                     /gene="RAB8A"
                     /gene_synonym="MEL; RAB8"
                     /inference="alignment:Splign:2.1.0"
     exon            338..427
                     /gene="RAB8A"
                     /gene_synonym="MEL; RAB8"
                     /inference="alignment:Splign:2.1.0"
     exon            428..493
                     /gene="RAB8A"
                     /gene_synonym="MEL; RAB8"
                     /inference="alignment:Splign:2.1.0"
     exon            494..544
                     /gene="RAB8A"
                     /gene_synonym="MEL; RAB8"
                     /inference="alignment:Splign:2.1.0"
     exon            545..2567
                     /gene="RAB8A"
                     /gene_synonym="MEL; RAB8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1945..1950
                     /regulatory_class="polyA_signal_sequence"
                     /gene="RAB8A"
                     /gene_synonym="MEL; RAB8"
                     /note="hexamer: ATTAAA"
     polyA_site      1968
                     /gene="RAB8A"
                     /gene_synonym="MEL; RAB8"
                     /note="major polyA site"
     polyA_site      2567
                     /gene="RAB8A"
                     /gene_synonym="MEL; RAB8"
ORIGIN      
        1 gagagagtgt aatatggcga agacctacga ttacctgttc aagctgctgc tgatcgggga
       61 ctcgggggtg gggaagacct gtgtcctgtt ccgcttctcc gaggacgcct tcaactccac
      121 ttttatctcc accataggaa ttgactttaa aattaggacc atagagctcg atggcaagag
      181 aattaaactg cagatatggg acacagccgg tcaggaacgg tttcggacga tcacaacggc
      241 ctactacagg ggtgcaatgg gcatcatgct ggtctacgac atcaccaacg agaagtcctt
      301 cgacaacatc cggaactgga ttcgcaacat tgaggagcac gcctctgcag acgtcgaaaa
      361 gatgatactc gggaacaagt gtgatgtgaa tgacaagaga caagtttcca aggaacgggg
      421 agaaaagctg gccctcgact atggaatcaa gttcatggag accagcgcga aggccaacat
      481 caatgtggaa aatgcatttt tcactctcgc cagagatatc aaagcaaaaa tggacaaaaa
      541 attggaaggc aacagccccc aggggagcaa ccagggagtc aaaatcacac cggaccagca
      601 gaagaggagc agctttttcc gatgtgttct tctgtgagga acaccgcctt actctgagcc
      661 tcgctcagcc cagctgactg tgcctgttct gagtgagccc ctcactcagc cggggccctc
      721 ccacctccaa cgccccgccc acgccgcggc caccgggccc acggccacca gaatgcaatt
      781 gagaaatcgt ttattttagt aactgtctga tctttttcaa ctttggagat ggaataagtt
      841 aaaaatttgc tatttttcct gtaacatctg ctgaacgggc ccacccacac gttgtatatt
      901 cagagagaga gagggagtca aggtgtgacc gtcgaccaca gccagtgtca ggcctctgcc
      961 tctgggcctt tgctttgtgg cctcactgca acacaaagct ccaccaggag gctggttcac
     1021 gtcccctacc acggaagcga ggtcccagaa ggccagcggt ggttccagga gcaacagctc
     1081 ccaaaccctg agcaaggcaa ccgatcgcca ggaccaggaa gcatcaccca ggagatttgg
     1141 cgcccacttc cacctcttct ctcagttttg gacaagtgac aaaccatttt gccccctcac
     1201 tcttcttttt taactgttaa accaaaggaa agcacaaatg aaggaaatcc tgtgtaaagc
     1261 attgagaagg aaagaagcct ggagcagcct ctcctgtcca cagccagggg ttaggtctgc
     1321 aggcccgtct gcggtcccca tcgagcatca aggggacgct gtgtgtgctg caagtgaccc
     1381 cgaaaacaac cacagccgtc acatggtcct cctgaagttg gggcaccctc ctctcagcac
     1441 caaaatggcc cccactcctt cgtgtcctcc cgctatctcc aaatcggacg ttctttctag
     1501 ctgagatttt tatttttcca catctgtagt gccatgaagc gattctgtct ttgacttcca
     1561 atggcaaacc tgggtgatcg ggaacaagca cgttgtaccc ttggctggac atggccaaga
     1621 cacaaggcat tccacggcgg caagctgacc gcacagcagt ctggcttgat tttcagccgt
     1681 catcattggg ttctgttttg acagctctgc tgtcccatag ggactgcgac tgggaccagg
     1741 tcaaccacgc caggggggtg tcaccagcct tttctttttt tctttctttt tttttttttc
     1801 ctccttaagc tgctgtcaat ccaaaccatt ggcatcatcg tttcttttga attaaaacca
     1861 acatatcagc aatagtctgc tctccccggg aatctctaac atgctctgtt tacatcgata
     1921 aatgcactta aggaaaacaa acaaattaaa gctcatctta aagtccaagg atttttatgt
     1981 ccacattttc cctgtaggcc acacggacat ggccaggtgc tgggggtggg tctggacaag
     2041 atggtagagc ccatggatta ccccatcgag cggccacctc agaggagatg ccacccatca
     2101 tcaaaagact aaattgggaa aagaagcctc ctggcttggt gcagtggctc acacctgtaa
     2161 tcccagcact ctgggaggcc aaggcgggct gatcacttga gctcaggtgt tcgagaccag
     2221 cctggccaac atggtgagcc tgcgtcacct ggcttctggc acacagctcg gccactgtgt
     2281 ccctcctgcc tccctggcag gtccttctag gggccctctc ctggctggct ttccagggca
     2341 ttccttccac tctatccagg gtccttccca agaccacacc caggtccagt cattccctag
     2401 gacttggcag agagctgtac tcacagccaa gatcacagca aaatcagcaa agggaaaagg
     2461 catgcagagt gaagtccaga ggcaaccaga cagaagcatc cagaatcctc tcacagtggg
     2521 gtcacacacc ccatgcttaa ctcccccaac aatgagttgt aacaaca
//
