LOCUS       NM_024638               4023 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens queuine tRNA-ribosyltransferase accessory subunit 2
            (QTRT2), transcript variant 1, mRNA.
ACCESSION   NM_024638
VERSION     NM_024638.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Sievers,K., Welp,L., Urlaub,H. and Ficner,R.
  TITLE     Structural and functional insights into human tRNA guanine
            transgylcosylase
  JOURNAL   RNA Biol 18 (sup1), 382-396 (2021)
   PUBMED   34241577
REFERENCE   3  (bases 1 to 4023)
  AUTHORS   Chen,Y.C., Brooks,A.F., Goodenough-Lashua,D.M., Kittendorf,J.D.,
            Showalter,H.D. and Garcia,G.A.
  TITLE     Evolution of eukaryal tRNA-guanine transglycosylase: insight gained
            from the heterocyclic substrate recognition by the wild-type and
            mutant human and Escherichia coli tRNA-guanine transglycosylases
  JOURNAL   Nucleic Acids Res 39 (7), 2834-2844 (2011)
   PUBMED   21131277
REFERENCE   4  (bases 1 to 4023)
  AUTHORS   Chen,Y.C., Kelly,V.P., Stachura,S.V. and Garcia,G.A.
  TITLE     Characterization of the human tRNA-guanine transglycosylase:
            confirmation of the heterodimeric subunit structure
  JOURNAL   RNA 16 (5), 958-968 (2010)
   PUBMED   20354154
  REMARK    GeneRIF: TGT is composed of a catalytic subunit, QTRT1, and QTRTD1,
            not USP14. QTRTD1 has been implicated as the salvage enzyme that
            generates free queuine from QMP.
REFERENCE   5  (bases 1 to 4023)
  AUTHORS   Boland,C., Hayes,P., Santa-Maria,I., Nishimura,S. and Kelly,V.P.
  TITLE     Queuosine formation in eukaryotic tRNA occurs via a
            mitochondria-localized heteromeric transglycosylase
  JOURNAL   J Biol Chem 284 (27), 18218-18227 (2009)
   PUBMED   19414587
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DC400836.1, BC039265.1 and
            BC034559.2.
            
            On Nov 23, 2018 this sequence version replaced NM_024638.3.
            
            Summary: This gene encodes a subunit of tRNA-guanine
            transglycosylase. tRNA-guanine transglycosylase is a heterodimeric
            enzyme complex that plays a critical role in tRNA modification by
            synthesizing the 7-deazaguanosine queuosine, which is found in
            tRNAs that code for asparagine, aspartic acid, histidine, and
            tyrosine. The encoded protein may play a role in the queuosine
            5'-monophosphate salvage pathway. Alternatively spliced transcript
            variants encoding multiple isoforms have been observed for this
            gene. [provided by RefSeq, Feb 2012].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC039265.1, SRR1803617.78016.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000281273.8/ ENSP00000281273.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                DC400836.1         3-29
            28-3105             BC039265.1         1-3078
            3106-3950           BC034559.2         2982-3826
            3951-4023           BC039265.1         3924-3996
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q13.31"
     gene            1..4023
                     /gene="QTRT2"
                     /gene_synonym="QTRTD1"
                     /note="queuine tRNA-ribosyltransferase accessory subunit
                     2"
                     /db_xref="GeneID:79691"
                     /db_xref="HGNC:HGNC:25771"
     exon            1..128
                     /gene="QTRT2"
                     /gene_synonym="QTRTD1"
                     /inference="alignment:Splign:2.1.0"
     exon            129..236
                     /gene="QTRT2"
                     /gene_synonym="QTRTD1"
                     /inference="alignment:Splign:2.1.0"
     exon            237..457
                     /gene="QTRT2"
                     /gene_synonym="QTRTD1"
                     /inference="alignment:Splign:2.1.0"
     CDS             258..1505
                     /gene="QTRT2"
                     /gene_synonym="QTRTD1"
                     /EC_number="2.4.2.29"
                     /note="isoform 1 is encoded by transcript variant 1;
                     queuine tRNA-ribosyltransferase domain containing 1"
                     /codon_start=1
                     /product="queuine tRNA-ribosyltransferase accessory
                     subunit 2 isoform 1"
                     /protein_id="NP_078914.1"
                     /db_xref="CCDS:CCDS33828.1"
                     /db_xref="GeneID:79691"
                     /db_xref="HGNC:HGNC:25771"
                     /translation="MKLSLTKVVNGCRLGKIKNLGKTGDHTMDIPGCLLYTKTGSAPH
                     LTHHTLHNIHGVPAMAQLTLSSLAEHHEVLTEYKEGVGKFIGMPESLLYCSLHDPVSP
                     CPAGYVTNKSVSVWSVAGRVEMTVSKFMAIQKALQPDWFQCLSDGEVSCKEATSIKRV
                     RKSVDRSLLFLDNCLRLQEESEVLQKSVIIGVIEGGDVMEERLRSARETAKRPVGGFL
                     LDGFQGNPTTLEARLRLLSSVTAELPEDKPRLISGVSRPDEVLECIERGVDLFESFFP
                     YQVTERGCALTFSFDYQPNPEETLLQQNGTQEEIKCMDQIKKIETTGCNQEITSFEIN
                     LKEKKYQEDFNPLVRGCSCYCCKNHTRAYIHHLLVTNELLAGVLLMMHNFEHYFGFFH
                     YIREALKSDKLAQLKELIHRQAS"
     exon            458..513
                     /gene="QTRT2"
                     /gene_synonym="QTRTD1"
                     /inference="alignment:Splign:2.1.0"
     exon            514..590
                     /gene="QTRT2"
                     /gene_synonym="QTRTD1"
                     /inference="alignment:Splign:2.1.0"
     exon            591..803
                     /gene="QTRT2"
                     /gene_synonym="QTRTD1"
                     /inference="alignment:Splign:2.1.0"
     exon            804..1003
                     /gene="QTRT2"
                     /gene_synonym="QTRTD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1004..1155
                     /gene="QTRT2"
                     /gene_synonym="QTRTD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1273
                     /gene="QTRT2"
                     /gene_synonym="QTRTD1"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..4023
                     /gene="QTRT2"
                     /gene_synonym="QTRTD1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3926..3931
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QTRT2"
                     /gene_synonym="QTRTD1"
                     /note="hexamer: AATAAA"
     polyA_site      3950
                     /gene="QTRT2"
                     /gene_synonym="QTRTD1"
                     /note="major polyA site"
     regulatory      4000..4005
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QTRT2"
                     /gene_synonym="QTRTD1"
                     /note="hexamer: ATTAAA"
     polyA_site      4023
                     /gene="QTRT2"
                     /gene_synonym="QTRTD1"
ORIGIN      
        1 agtactccct gattggctct gcactggagg cagtgatttt ggggcagaga attttgcaac
       61 acgtggtagt gaactgtgag gagtttgagg ggtctgaaga ctgaaagagt cgaatggttt
      121 gttggcaggg tgtcctggtg gattggtttc tgtaagttca gattctcata aatcgtgtga
      181 gcgtcgccga cacctctgag ataaaagggc ccctttcgac tagcctctgc tgaaaggacc
      241 tagaagaatc ccttaggatg aagctgagtc ttaccaaggt agttaatggc tgtcgcctag
      301 gaaaaataaa aaacctgggc aaaacagggg accacaccat ggatattcca ggctgccttc
      361 tgtataccaa gactggctcc gccccacacc tcacccatca cacgctgcat aatatccacg
      421 gggttcctgc catggctcag cttacgctgt catccctagc agaacatcat gaagtcttga
      481 cagaatataa agaaggagtt ggaaagttta taggcatgcc agaatcactc ttgtactgct
      541 ccctgcacga tccagtcagc ccctgcccgg ctggttatgt aacaaacaag tctgtgtctg
      601 tgtggagtgt tgcaggacga gtggaaatga ctgtttccaa gttcatggca attcagaagg
      661 cccttcagcc agactggttc cagtgcctct ccgatggaga agtatcttgt aaggaagcaa
      721 cttccataaa aagggtcaga aagtctgttg accgatcact tcttttcttg gataactgtc
      781 tgcggctgca ggaagagtca gaggttcttc agaagagtgt gatcattgga gtgattgaag
      841 gtggagatgt gatggaagag aggctgaggt cagcacgaga gacagccaag cggcctgtgg
      901 gtggcttcct tctggatggt tttcaaggaa atccaacaac cctggaggct agactacgct
      961 tgctgtcatc agtcactgca gagctgccgg aggacaagcc aaggctcata tctggtgtta
     1021 gtcggccaga tgaggtgctc gagtgtattg aaagaggagt ggacttattt gagagttttt
     1081 tcccttatca agtaacagag cggggatgtg ccctgacttt cagttttgat taccagccga
     1141 atcctgaaga gacactacta caacaaaatg gaacacaaga agaaataaaa tgtatggatc
     1201 aaataaagaa aattgaaaca actggttgca accaagaaat aacatcattt gaaattaatc
     1261 tgaaggaaaa aaagtaccag gaggacttta acccgctggt gagaggatgt tcctgttact
     1321 gctgtaagaa tcacactcgg gcatacatcc accatctgct ggtgaccaat gagctgctgg
     1381 ccggagtcct gcttatgatg cacaactttg aacactactt tgggtttttc cattacatcc
     1441 gggaagcact aaaaagtgac aaactggcac agttgaaaga gctcatccac aggcaagcat
     1501 cttgagatct tgcaaataca agtctcactc ttcacactga gcctgtacca ctgttgtaac
     1561 atgggaagac gtgaagaaga aataatctga gctttaatta tttatatttg gatataaggt
     1621 ctgcttaaat aaagaatctt tgtaccaaac tgcccacatg agggtgaaga gatttcctca
     1681 aaagacttaa atgacctgga ttgatcagag agaattgaac tgtgaccttt aagacttcta
     1741 gactaattct tttagttgat agagattcat ttagtcaaag acaaaagctt taactgtgag
     1801 ggcacagcct tgaagtggga gtgatgagat tctgagggac ccatgaattg gattgaggct
     1861 tgaggggaaa tggtgtgaga cgaatgggct ctggacatat gcctgttgat ttgagaagaa
     1921 atctggctgg tttgagggtt tcctttagtt caccctcata ctctcaggag actcttctgg
     1981 atacttttgt cttccaccct gccctggcag tgcagccaat cagaaatggc tcttgtgact
     2041 taactgggct tggatatccc tggaatgtgg ggcttgaaca ttgctcctgt gatgatcatc
     2101 tgtcccaggt cacactcctt catttgacca catggcctat ttgcaacttc tttttagagc
     2161 caatgtaatt gcctgttagg agccagaagt ttgcccagct gctttcttct ggttgtacag
     2221 atccattgtg gtctgccttc caggctcatt aatagtctaa ctacgtaata actgaagacc
     2281 tatctcttgt gaagattcat tctcaatgtg ttcacttgga cagagccctg cctggcctaa
     2341 cgattagagg gttagtctcc atcctgctca gatatgactc ctgggcaatt cacttaactt
     2401 ctccatggct gcttcctgct gaataaatag ggacaggcat aatggatagg aatgaatgaa
     2461 cttttaagat actgtctagc tctaaaattg gaagaacaaa aagttttaga ttagagtcat
     2521 agccttaata gccctagttg tcatcctggg agacaggcaa cagtagagat atttgagagc
     2581 ctaaagagag gtttggcctg tgggttttta agtggttatt gaattggtat caggagatcc
     2641 tgaggctggt aggggaaggt aattctttct aagttacctc tgtatttttc aagttttcta
     2701 taaggaatac acatacaccc acatgcacac accatagttt ttatacaaac agcaataaca
     2761 aaaccaaaaa gatgcccctt tttttgtagg gataagaaat acatttgttt tatacttcta
     2821 tgctatattt tgctattcaa aatttagtgg gcattactta acattgtttc taattatttt
     2881 gtggctgctg tatgttttat gtgttgggag cccattgtat taggccgttc ttggattgct
     2941 ataaagaaat acctgagact gggtaatttg gttttttggt tttttggggt tttttttgag
     3001 acggagcctt gctctgtcgc ccaggctgga gtgcagtggc gcgatctcgg ctctatgcaa
     3061 cctccacctc ctgggttcat gccattctcc tgcctcagcc tcccgagtag ctgggactac
     3121 aggcgcccgc caccatgcct ggctaatttt ttatattttt agtagagatg gggtttcacc
     3181 gtatgagcca ggatggtctc gatctcctga cctcatgatc cacaagactg ggtaatctgt
     3241 aaagaaaaga ggtttaattg gctcatggct ctgcaggctt tacaggaagc atggtactgg
     3301 catctgcttg gtttctgggg aggcctccgg aagcttatag tcatgacaga aggtgaaggg
     3361 ggagcaggca catcacatga caaaagcagg aacaagactc agagcaagaa gtcacttatc
     3421 accaagggga tggcccaggc cattcatgag ggatccacct ttgtgctcca aacacctccc
     3481 accaggcccc acctccagta ttggggatta catttcaact tgaggtttgg gcgggaacaa
     3541 atagtcaaat tatattaccc atctacatgg ttttcttttt cttagtatgg gagtgtccat
     3601 gaataaattc atgggcatcc tcataactct tccatgaatg gcaaggggta cttatggaaa
     3661 acagttttcc aaagatctga ttgttttgaa atgtgttaga ttggacttgt tcaaatgttc
     3721 atatttttgt tctatgctaa atgcatcgtt aggggagtag tggtaaatat tccagaatcc
     3781 atatgaaatt catgagttta tttatgtcta aaacactgaa aactgtgtcc aatatcgttc
     3841 agtttcctgg gtctttttct tattagcaca tttgagaaaa atattccttt cctttattgt
     3901 agtaaatcta ttaaaggcaa tagccaataa aacattttta attttaaaaa tttactgtgt
     3961 ttggctttct gtaaatgtag taggtctgtt aaacaaaata ttaaatttcc atagtaccag
     4021 aaa
//
