LOCUS       NM_031209               1329 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens queuine tRNA-ribosyltransferase catalytic subunit 1
            (QTRT1), mRNA.
ACCESSION   NM_031209
VERSION     NM_031209.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Sievers,K., Neumann,P., Susac,L., Da Vela,S., Graewert,M.,
            Trowitzsch,S., Svergun,D., Tampe,R. and Ficner,R.
  TITLE     Structural and functional insights into tRNA recognition by human
            tRNA guanine transglycosylase
  JOURNAL   Structure 32 (3), 316-327 (2024)
   PUBMED   38181786
  REMARK    GeneRIF: Structural and functional insights into tRNA recognition
            by human tRNA guanine transglycosylase.
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 1329)
  AUTHORS   Sievers,K., Welp,L., Urlaub,H. and Ficner,R.
  TITLE     Structural and functional insights into human tRNA guanine
            transgylcosylase
  JOURNAL   RNA Biol 18 (sup1), 382-396 (2021)
   PUBMED   34241577
REFERENCE   4  (bases 1 to 1329)
  AUTHORS   Fergus,C., Al-Qasem,M., Cotter,M., McDonnell,C.M., Sorrentino,E.,
            Chevot,F., Hokamp,K., Senge,M.O., Southern,J.M., Connon,S.J. and
            Kelly,V.P.
  TITLE     The human tRNA-guanine transglycosylase displays promiscuous
            nucleobase preference but strict tRNA specificity
  JOURNAL   Nucleic Acids Res 49 (9), 4877-4890 (2021)
   PUBMED   34009357
  REMARK    GeneRIF: The human tRNA-guanine transglycosylase displays
            promiscuous nucleobase preference but strict tRNA specificity.
REFERENCE   5  (bases 1 to 1329)
  AUTHORS   Biela,I., Tidten-Luksch,N., Immekus,F., Glinca,S., Nguyen,T.X.,
            Gerber,H.D., Heine,A., Klebe,G. and Reuter,K.
  TITLE     Investigation of specificity determinants in bacterial tRNA-guanine
            transglycosylase reveals queuine, the substrate of its eucaryotic
            counterpart, as inhibitor
  JOURNAL   PLoS One 8 (5), e64240 (2013)
   PUBMED   23704982
  REMARK    GeneRIF: Homology models of C elegans Tgt and human Tgt suggest
            that the replacement of Cys158 and Val233 in bacterial Tgt
            (Zymomonas mobilis) by valine and accordingly glycine in eucaryotic
            Tgt largely accounts for the different substrate specificities.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1329)
  AUTHORS   Eric Thomas,C., Chen,Y.C. and Garcia,G.A.
  TITLE     Differential heterocyclic substrate recognition by, and pteridine
            inhibition of E. coli and human tRNA-guanine transglycosylases
  JOURNAL   Biochem Biophys Res Commun 410 (1), 34-39 (2011)
   PUBMED   21640076
  REMARK    GeneRIF: the inhibition of the human TGT by biopterin, consistent
            with earlier reports on other eukaryal TGTs, and supportive of the
            concept that pteridines may regulate eukaryal TGT activity in vivo.
REFERENCE   7  (bases 1 to 1329)
  AUTHORS   Chen,Y.C., Brooks,A.F., Goodenough-Lashua,D.M., Kittendorf,J.D.,
            Showalter,H.D. and Garcia,G.A.
  TITLE     Evolution of eukaryal tRNA-guanine transglycosylase: insight gained
            from the heterocyclic substrate recognition by the wild-type and
            mutant human and Escherichia coli tRNA-guanine transglycosylases
  JOURNAL   Nucleic Acids Res 39 (7), 2834-2844 (2011)
   PUBMED   21131277
  REMARK    GeneRIF: phylogenetic and kinetic analyses support the conclusion
            that all tRNA-guanine transglycosylases have divergently evolved to
            specifically recognize their cognate heterocyclic substrates.
REFERENCE   8  (bases 1 to 1329)
  AUTHORS   Chen,Y.C., Kelly,V.P., Stachura,S.V. and Garcia,G.A.
  TITLE     Characterization of the human tRNA-guanine transglycosylase:
            confirmation of the heterodimeric subunit structure
  JOURNAL   RNA 16 (5), 958-968 (2010)
   PUBMED   20354154
  REMARK    GeneRIF: TGT is composed of a catalytic subunit, QTRT1, and QTRTD1,
            not USP14. QTRTD1 has been implicated as the salvage enzyme that
            generates free queuine from QMP.
REFERENCE   9  (bases 1 to 1329)
  AUTHORS   Boland,C., Hayes,P., Santa-Maria,I., Nishimura,S. and Kelly,V.P.
  TITLE     Queuosine formation in eukaryotic tRNA occurs via a
            mitochondria-localized heteromeric transglycosylase
  JOURNAL   J Biol Chem 284 (27), 18218-18227 (2009)
   PUBMED   19414587
REFERENCE   10 (bases 1 to 1329)
  AUTHORS   Deshpande,K.L. and Katze,J.R.
  TITLE     Characterization of cDNA encoding the human tRNA-guanine
            transglycosylase (TGT) catalytic subunit
  JOURNAL   Gene 265 (1-2), 205-212 (2001)
   PUBMED   11255023
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CT003728.1, AF302783.1 and
            AF370379.1.
            
            On Nov 23, 2018 this sequence version replaced NM_031209.2.
            
            Summary: This gene encodes the catalytic subunit of tRNA-guanine
            transglycosylase. tRNA-guanine transglycosylase is a heterodimeric
            enzyme complex that plays a critical role in tRNA modification by
            synthesizing the 7-deazaguanosine queuosine, which is found in
            tRNAs that code for asparagine, aspartic acid, histidine and
            tyrosine. A pseudogene of this gene is located on the long arm of
            chromosome X. [provided by RefSeq, Feb 2012].
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1163655.657272.1, AF302783.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000250237.10/ ENSP00000250237.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                CT003728.1         4-19
            17-1227             AF302783.1         1-1211
            1228-1329           AF370379.1         1408-1509
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19p13.2"
     gene            1..1329
                     /gene="QTRT1"
                     /gene_synonym="FP3235; TGT; TGUT"
                     /note="queuine tRNA-ribosyltransferase catalytic subunit
                     1"
                     /db_xref="GeneID:81890"
                     /db_xref="HGNC:HGNC:23797"
                     /db_xref="MIM:609615"
     exon            1..265
                     /gene="QTRT1"
                     /gene_synonym="FP3235; TGT; TGUT"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..1234
                     /gene="QTRT1"
                     /gene_synonym="FP3235; TGT; TGUT"
                     /EC_number="2.4.2.64"
                     /note="tRNA-guanine transglycosylase; guanine insertion
                     enzyme; TGT, 43-KD subunit; TGT, catalytic subunit;
                     queuine tRNA-ribosyltransferase 1"
                     /codon_start=1
                     /product="queuine tRNA-ribosyltransferase catalytic
                     subunit 1"
                     /protein_id="NP_112486.1"
                     /db_xref="CCDS:CCDS12248.1"
                     /db_xref="GeneID:81890"
                     /db_xref="HGNC:HGNC:23797"
                     /db_xref="MIM:609615"
                     /translation="MAGAATQASLESAPRIMRLVAECSRSRARAGELWLPHGTVATPV
                     FMPVGTQATMKGITTEQLDALGCRICLGNTYHLGLRPGPELIQKANGLHGFMNWPHNL
                     LTDSGGFQMVSLVSLSEVTEEGVRFRSPYDGNETLLSPEKSVQIQNALGSDIIMQLDD
                     VVSSTVTGPRVEEAMYRSIRWLDRCIAAHQRPDKQNLFAIIQGGLDADLRATCLEEMT
                     KRDVPGFAIGGLSGGESKSQFWRMVALSTSRLPKDKPRYLMGVGYATDLVVCVALGCD
                     MFDCVFPTRTARFGSALVPTGNLQLRKKVFEKDFGPIDPECTCPTCQKHSRAFLHALL
                     HSDNTAALHHLTVHNIAYQLQLMSAVRTSIVEKRFPDFVRDFMGAMYGDPTLCPTWAT
                     DALASVGITLG"
     exon            266..334
                     /gene="QTRT1"
                     /gene_synonym="FP3235; TGT; TGUT"
                     /inference="alignment:Splign:2.1.0"
     exon            335..473
                     /gene="QTRT1"
                     /gene_synonym="FP3235; TGT; TGUT"
                     /inference="alignment:Splign:2.1.0"
     exon            474..552
                     /gene="QTRT1"
                     /gene_synonym="FP3235; TGT; TGUT"
                     /inference="alignment:Splign:2.1.0"
     exon            553..668
                     /gene="QTRT1"
                     /gene_synonym="FP3235; TGT; TGUT"
                     /inference="alignment:Splign:2.1.0"
     exon            669..807
                     /gene="QTRT1"
                     /gene_synonym="FP3235; TGT; TGUT"
                     /inference="alignment:Splign:2.1.0"
     exon            808..883
                     /gene="QTRT1"
                     /gene_synonym="FP3235; TGT; TGUT"
                     /inference="alignment:Splign:2.1.0"
     exon            884..993
                     /gene="QTRT1"
                     /gene_synonym="FP3235; TGT; TGUT"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1081
                     /gene="QTRT1"
                     /gene_synonym="FP3235; TGT; TGUT"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1329
                     /gene="QTRT1"
                     /gene_synonym="FP3235; TGT; TGUT"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      1329
                     /gene="QTRT1"
                     /gene_synonym="FP3235; TGT; TGUT"
                     /note="major polyA site"
ORIGIN      
        1 ccacgtggtt ccgacagtca agatggcggg agcagctacc caggcttccc tggagtcggc
       61 cccacggatc atgcggctgg tggccgaatg cagccgctcc agggcccggg caggcgagct
      121 gtggctgccg catgggacag tggccactcc tgtgttcatg ccagtgggca cgcaggccac
      181 catgaagggc atcacgaccg aacagctgga cgctctgggt tgccgcatct gcctgggcaa
      241 tacctaccat ctgggtctaa ggccgggacc cgagctgatc cagaaagcca acggtctcca
      301 cggcttcatg aattggcctc ataatctgct aacggacagc ggcggtttcc agatggtgtc
      361 gctggtgtct ctgtccgagg tgacggagga gggcgtccgc ttccgctccc cctacgacgg
      421 caatgagacc ctgctgagcc cggagaaatc cgtgcagatc cagaatgcgc tgggctcgga
      481 catcatcatg cagctggacg acgtggttag cagtactgtg actgggccac gtgtggagga
      541 ggccatgtac aggtcaatcc gctggctgga ccggtgcatt gcagcccatc agcggccgga
      601 caagcagaac ctcttcgcca ttatccaggg tgggctggac gcagatctcc gggccacctg
      661 ccttgaagag atgaccaagc gagacgtgcc tggcttcgcc atcgggggcc tgagcggggg
      721 tgagagcaag tcgcagttct ggcggatggt ggcgctgagc acctctcggc tgccgaagga
      781 caagccccga tatctgatgg gggttggcta tgccactgat ctggtagtct gcgtggctct
      841 tggatgtgac atgttcgact gcgtcttccc cacacggaca gcgcgctttg gctctgccct
      901 ggtgcccact gggaacctgc agttgaggaa gaaggtgttt gagaaggact tcggccccat
      961 agacccggag tgcacctgcc ccacgtgcca aaagcacagc cgcgccttcc tgcacgcact
     1021 gctgcacagt gacaacacgg ccgcgctgca ccacctcacg gtccacaaca tcgcctacca
     1081 gctgcagctc atgagcgccg tccgcaccag catcgtggag aagcgcttcc cggacttcgt
     1141 gcgggacttc atgggcgcca tgtacgggga tcccaccctc tgtcccacct gggccactga
     1201 cgctctggcc tctgtgggaa tcacactggg ctgacctggc attgggagag ggagggagga
     1261 aggaagggag ggaggggctg gaagatactg aaggattcct ttttgaaagg ttttttttat
     1321 tgtaactta
//
