LOCUS       NM_002826               9276 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens quiescin sulfhydryl oxidase 1 (QSOX1), transcript
            variant 1, mRNA.
ACCESSION   NM_002826 NM_152662
VERSION     NM_002826.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Wang,Y., Wei,J., Feng,L., Li,O., Huang,L., Zhou,S., Xu,Y., An,K.,
            Zhang,Y., Chen,R., He,L., Wang,Q., Wang,H., Du,Y., Liu,R.,
            Huang,C., Zhang,X., Yang,Y.G., Kan,Q. and Tian,X.
  TITLE     Aberrant m5C hypermethylation mediates intrinsic resistance to
            gefitinib through NSUN2/YBX1/QSOX1 axis in EGFR-mutant
            non-small-cell lung cancer
  JOURNAL   Mol Cancer 22 (1), 81 (2023)
   PUBMED   37161388
  REMARK    GeneRIF: Aberrant m5C hypermethylation mediates intrinsic
            resistance to gefitinib through NSUN2/YBX1/QSOX1 axis in
            EGFR-mutant non-small-cell lung cancer.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9276)
  AUTHORS   Sun,J., Zhou,C., Zhao,Y., Zhang,X., Chen,W., Zhou,Q., Hu,B.,
            Gao,D., Raatz,L., Wang,Z., Nelson,P.J., Jiang,Y., Ren,N.,
            Bruns,C.J. and Zhou,H.
  TITLE     Quiescin sulfhydryl oxidase 1 promotes sorafenib-induced
            ferroptosis in hepatocellular carcinoma by driving EGFR endosomal
            trafficking and inhibiting NRF2 activation
  JOURNAL   Redox Biol 41, 101942 (2021)
   PUBMED   33770521
  REMARK    GeneRIF: Quiescin sulfhydryl oxidase 1 promotes sorafenib-induced
            ferroptosis in hepatocellular carcinoma by driving EGFR endosomal
            trafficking and inhibiting NRF2 activation.
REFERENCE   3  (bases 1 to 9276)
  AUTHORS   Ma,Q., Yu,M., Zhou,B. and Zhou,H.
  TITLE     QSOX1 promotes mitochondrial apoptosis of hepatocellular carcinoma
            cells during anchorage-independent growth by inhibiting lipid
            synthesis
  JOURNAL   Biochem Biophys Res Commun 532 (2), 258-264 (2020)
   PUBMED   32863002
  REMARK    GeneRIF: QSOX1 promotes mitochondrial apoptosis of hepatocellular
            carcinoma cells during anchorage-independent growth by inhibiting
            lipid synthesis.
REFERENCE   4  (bases 1 to 9276)
  AUTHORS   Javitt,G., Grossman-Haham,I., Alon,A., Resnick,E., Mutsafi,Y.,
            Ilani,T. and Fass,D.
  TITLE     cis-Proline mutants of quiescin sulfhydryl oxidase 1 with altered
            redox properties undermine extracellular matrix integrity and cell
            adhesion in fibroblast cultures
  JOURNAL   Protein Sci 28 (1), 228-238 (2019)
   PUBMED   30367560
  REMARK    GeneRIF: mutation of a conserved cis-proline amino acid, analogous
            to a mutation used to trap substrates of a bacterial disulfide
            catalyst, has a dramatic effect on the physiological function of
            the mammalian disulfide catalyst QSOX1.
REFERENCE   5  (bases 1 to 9276)
  AUTHORS   Sung,H.J., Ahn,J.M., Yoon,Y.H., Na,S.S., Choi,Y.J., Kim,Y.I.,
            Lee,S.Y., Lee,E.B., Cho,S. and Cho,J.Y.
  TITLE     Quiescin Sulfhydryl Oxidase 1 (QSOX1) Secreted by Lung Cancer Cells
            Promotes Cancer Metastasis
  JOURNAL   Int J Mol Sci 19 (10), 3213 (2018)
   PUBMED   30336636
  REMARK    GeneRIF: QSOX1 might be a lung cancer tissue-derived biomarker and
            be involved in the promotion of lung cancers, and thus can be a
            therapeutic target for lung cancers.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 9276)
  AUTHORS   Thorpe,C., Hoober,K.L., Raje,S., Glynn,N.M., Burnside,J., Turi,G.K.
            and Coppock,D.L.
  TITLE     Sulfhydryl oxidases: emerging catalysts of protein disulfide bond
            formation in eukaryotes
  JOURNAL   Arch Biochem Biophys 405 (1), 1-12 (2002)
   PUBMED   12176051
  REMARK    Review article
REFERENCE   7  (bases 1 to 9276)
  AUTHORS   Coppock,D., Kopman,C., Gudas,J. and Cina-Poppe,D.A.
  TITLE     Regulation of the quiescence-induced genes: quiescin Q6, decorin,
            and ribosomal protein S29
  JOURNAL   Biochem Biophys Res Commun 269 (2), 604-610 (2000)
   PUBMED   10708601
REFERENCE   8  (bases 1 to 9276)
  AUTHORS   Hoober,K.L., Glynn,N.M., Burnside,J., Coppock,D.L. and Thorpe,C.
  TITLE     Homology between egg white sulfhydryl oxidase and quiescin Q6
            defines a new class of flavin-linked sulfhydryl oxidases
  JOURNAL   J Biol Chem 274 (45), 31759-31762 (1999)
   PUBMED   10542195
REFERENCE   9  (bases 1 to 9276)
  AUTHORS   Coppock,D.L., Cina-Poppe,D. and Gilleran,S.
  TITLE     The quiescin Q6 gene (QSCN6) is a fusion of two ancient gene
            families: thioredoxin and ERV1
  JOURNAL   Genomics 54 (3), 460-468 (1998)
   PUBMED   9878249
REFERENCE   10 (bases 1 to 9276)
  AUTHORS   Coppock,D.L., Kopman,C., Scandalis,S. and Gilleran,S.
  TITLE     Preferential gene expression in quiescent human lung fibroblasts
  JOURNAL   Cell Growth Differ 4 (6), 483-493 (1993)
   PUBMED   8396966
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from U97276.2, BX382719.2,
            BC017692.1, L42379.1 and AL390718.11.
            
            On Nov 23, 2018 this sequence version replaced NM_002826.4.
            
            Summary: This gene encodes a protein that contains domains of
            thioredoxin and ERV1, members of two long-standing gene families.
            The gene expression is induced as fibroblasts begin to exit the
            proliferative cycle and enter quiescence, suggesting that this gene
            plays an important role in growth regulation. Two transcript
            variants encoding two different isoforms have been found for this
            gene. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Sequence Note: The 3' UTR of this gene may extend about 2 kbp
            further to another polyA site.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U97276.2, SRR3476690.853351.1
                                           [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000367602.8/ ENSP00000356574.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-473               U97276.2           37-509
            474-528             BX382719.2         452-506
            529-637             BC017692.1         504-612
            638-683             L42379.1           607-652
            684-1265            BC017692.1         659-1240
            1266-1279           L42379.1           1234-1247
            1280-1845           BC017692.1         1255-1820
            1846-1919           L42379.1           1812-1885
            1920-3278           U97276.2           1956-3314
            3279-3280           BC017692.1         2521-2522
            3281-9276           AL390718.11        147961-153956
FEATURES             Location/Qualifiers
     source          1..9276
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q25.2"
     gene            1..9276
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
                     /note="quiescin sulfhydryl oxidase 1"
                     /db_xref="GeneID:5768"
                     /db_xref="HGNC:HGNC:9756"
                     /db_xref="MIM:603120"
     exon            1..304
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
                     /inference="alignment:Splign:2.1.0"
     CDS             40..2283
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
                     /EC_number="1.8.3.2"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; sulfhydryl oxidase 1; quiescin Q6 sulfhydryl
                     oxidase 1; thiol oxidase 1; testis tissue sperm-binding
                     protein Li 62n"
                     /codon_start=1
                     /product="sulfhydryl oxidase 1 isoform a precursor"
                     /protein_id="NP_002817.2"
                     /db_xref="CCDS:CCDS1337.1"
                     /db_xref="GeneID:5768"
                     /db_xref="HGNC:HGNC:9756"
                     /db_xref="MIM:603120"
                     /translation="MRRCNSGSGPPPSLLLLLLWLLAVPGANAAPRSALYSPSDPLTL
                     LQADTVRGAVLGSRSAWAVEFFASWCGHCIAFAPTWKALAEDVKAWRPALYLAALDCA
                     EETNSAVCRDFNIPGFPTVRFFKAFTKNGSGAVFPVAGADVQTLRERLIDALESHHDT
                     WPPACPPLEPAKLEEIDGFFARNNEEYLALIFEKGGSYLGREVALDLSQHKGVAVRRV
                     LNTEANVVRKFGVTDFPSCYLLFRNGSVSRVPVLMESRSFYTAYLQRLSGLTREAAQT
                     TVAPTTANKIAPTVWKLADRSKIYMADLESALHYILRIEVGRFPVLEGQRLVALKKFV
                     AVLAKYFPGRPLVQNFLHSVNEWLKRQKRNKIPYSFFKTALDDRKEGAVLAKKVNWIG
                     CQGSEPHFRGFPCSLWVLFHFLTVQAARQNVDHSQEAAKAKEVLPAIRGYVHYFFGCR
                     DCASHFEQMAAASMHRVGSPNAAVLWLWSSHNRVNARLAGAPSEDPQFPKVQWPPREL
                     CSACHNERLDVPVWDVEATLNFLKAHFSPSNIILDFPAAGSAARRDVQNVAAAPELAM
                     GALELESRNSTLDPGKPEMMKSPTNTTPHVPAEGPEASRPPKLHPGLRAAPGQEPPEH
                     MAELQRNEQEQPLGQWHLSKRDTGAALLAESRAEKNRLWGPLEVRRVGRSSKQLVDIP
                     EGQLEARAGRGRGQWLQVLGGGFSYLDISLCVGLYSLSFMGLLAMYTYFQAKIRALKG
                     HAGHPAA"
     exon            305..405
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
                     /inference="alignment:Splign:2.1.0"
     exon            406..451
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
                     /inference="alignment:Splign:2.1.0"
     exon            452..554
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
                     /inference="alignment:Splign:2.1.0"
     exon            555..645
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
                     /inference="alignment:Splign:2.1.0"
     exon            646..791
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
                     /inference="alignment:Splign:2.1.0"
     exon            792..926
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
                     /inference="alignment:Splign:2.1.0"
     exon            927..1056
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
                     /inference="alignment:Splign:2.1.0"
     exon            1057..1179
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
                     /inference="alignment:Splign:2.1.0"
     exon            1180..1327
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
                     /inference="alignment:Splign:2.1.0"
     exon            1328..1507
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..9276
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3257..3262
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
                     /note="hexamer: AATAAA"
     polyA_site      3280
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
                     /note="major polyA site"
     regulatory      5520..5525
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
                     /note="hexamer: AATAAT"
     polyA_site      5541
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
     regulatory      5947..5952
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
                     /note="hexamer: AATACA"
     polyA_site      5971
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
     regulatory      9255..9260
                     /regulatory_class="polyA_signal_sequence"
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
                     /note="hexamer: AATAAA"
     polyA_site      9276
                     /gene="QSOX1"
                     /gene_synonym="Q6; QSCN6"
ORIGIN      
        1 atccggtgct tgcgtgtggt ggtgagcgca gcgccgagga tgaggaggtg caacagcggc
       61 tccgggccgc cgccgtcgct gctgctgctg ctgctgtggc tgctcgcggt tcccggcgct
      121 aacgcggccc cgcggtcggc gctctattcg ccttccgacc cgctgacgct gctgcaggcg
      181 gacacggtgc gcggcgcggt gctgggctcc cgcagcgcct gggccgtgga gttcttcgcc
      241 tcctggtgcg gccactgcat cgccttcgcc ccgacgtgga aggcgctggc cgaagacgtc
      301 aaagcctgga ggccggccct gtatctcgcc gccctggact gtgctgagga gaccaacagt
      361 gcagtctgca gagacttcaa catccctggc ttcccgactg tgaggttctt caaggccttt
      421 accaagaacg gctcgggagc agtatttcca gtggctggtg ctgacgtgca gacactgcgg
      481 gagaggctca ttgacgccct ggagtcccat catgacacgt ggcccccagc ctgtccccca
      541 ctggagcctg ccaagctgga ggagattgat ggattctttg cgagaaataa cgaagagtac
      601 ctggctctga tctttgaaaa gggaggctcc tacctgggta gagaggtggc tctggacctg
      661 tcccagcaca aaggcgtggc ggtgcgcagg gtgctgaaca cagaggccaa tgtggtgaga
      721 aagtttggtg tcaccgactt cccctcttgc tacctgctgt tccggaatgg ctctgtctcc
      781 cgagtccccg tgctcatgga atccaggtcc ttctataccg cttacctgca gagactctct
      841 gggctcacca gggaggctgc ccagaccaca gttgcaccaa ccactgctaa caagatagct
      901 cccactgttt ggaaattggc agatcgctcc aagatctaca tggctgacct ggaatctgca
      961 ctgcactaca tcctgcggat agaagtgggc aggttcccgg tcctggaagg gcagcgcctg
     1021 gtggccctga aaaagtttgt ggcagtgctg gccaagtatt tccctggccg gcccttagtc
     1081 cagaacttcc tgcactccgt gaatgaatgg ctcaagaggc agaagagaaa taaaattccc
     1141 tacagtttct ttaaaactgc cctggacgac aggaaagagg gtgccgttct tgccaagaag
     1201 gtgaactgga ttggctgcca ggggagtgag ccgcatttcc ggggctttcc ctgctccctg
     1261 tgggtcctct tccacttctt gactgtgcag gcagctcggc aaaatgtaga ccactcacag
     1321 gaagcagcca aggccaagga ggtcctccca gccatccgag gctacgtgca ctacttcttc
     1381 ggctgccgag actgcgctag ccacttcgag cagatggctg ctgcctccat gcaccgggtg
     1441 gggagtccca acgccgctgt cctctggctc tggtctagcc acaacagggt caatgctcgc
     1501 cttgcaggtg cccccagcga ggacccccag ttccccaagg tgcagtggcc accccgtgaa
     1561 ctttgttctg cctgccacaa tgaacgcctg gatgtgcccg tgtgggacgt ggaagccacc
     1621 ctcaacttcc tcaaggccca cttctcccca agcaacatca tcctggactt ccctgcagct
     1681 gggtcagctg cccggaggga tgtgcagaat gtggcagccg ccccagagct ggcgatggga
     1741 gccctggagc tggaaagccg gaattcaact ctggaccctg ggaagcctga gatgatgaag
     1801 tcccccacaa acaccacccc acatgtgccg gctgagggac ctgaggcaag tcgacccccg
     1861 aagctgcacc ctggcctcag agctgcacca ggccaggagc ctcctgagca catggcagag
     1921 cttcagagga atgagcagga gcagccgctt gggcagtggc acttgagcaa gcgagacaca
     1981 ggggctgcat tgctggctga gtccagggct gagaagaacc gcctctgggg ccctttggag
     2041 gtcaggcgcg tgggccgcag ctccaagcag ctggtcgaca tccctgaggg ccagctggag
     2101 gcccgagctg gacggggccg aggccagtgg ctgcaggtgc tgggaggggg cttctcttac
     2161 ctggacatca gcctctgtgt ggggctctat tccctgtcct tcatgggcct gctggccatg
     2221 tacacctact tccaggccaa gataagggcc ctgaagggcc atgctggcca ccctgcagcc
     2281 tgaaccacct ggggaggagg cgggagaggg agctgccatc tctaggcacc tcaagccccc
     2341 tgaccccatt ccctcccctc ccaccccttg ctccttgtct ggcctagaag tgtgggaaat
     2401 tcaggaaaac gagttgctcc agtgaagctt cttggggttg ctaggacaga gagctccttt
     2461 gacacaaaag acaggagcag ggtccaggtt cccctgctgt gcagggaggg cagccccggg
     2521 cagtgggcat agggcagctc agtccctggc ctcttagcac cacattcctg tttttcagct
     2581 tatttgaagt cctgcctcat tctcactgga gcctcagtct ctcctgcttg gtcttggccc
     2641 tcaactgggg caagtgaagc cagaggaggg tcccccagct gggtgggctg gaatggaact
     2701 cctcactagc tgctggggct ccgcccaccc tgctcccttc cggacaatga agaagccttt
     2761 gcaccctggg aggaaggacc accccgggcc ctctatgcct ggccagcctc cagctcctca
     2821 gacctcctgg gtggggtttg gcttcagggt ggggtttgga agcttctgga agtcgtgctg
     2881 gtctcccagg tgaggcaagc catggttgct gggctgtagg gtgagtggct tgcttggtgg
     2941 gacctgacga gttggtggca tgggaaggat gtgggtctct agtgccttgc cctggcttag
     3001 ctgcaggaga agatggctgc tttcacttcc ccccattgag ctctgctccc tctgagcctg
     3061 gtcttttgtc cttttttatt ttggtctcca agatgaatgc tcatctttgg agggtgccag
     3121 gtagaagcta gggaggggag tgtcttctct ctccaggttt caccttccag tgtgcagaag
     3181 ttagaagggt ctggcggggg cagtgcctta cacatgcttg attcccacgc taccccctgc
     3241 cttgggaggt gtgtggaata aattattttt gttaaggcaa gcattggtgt gttctttaac
     3301 ttgctacttg gagacctagt gtccagtctg gacagaatgc acagagacca gcctcacctg
     3361 gaatgcagcc agactcgatg tccctcagca cacacagctc ctggccacag actgcccata
     3421 gaactgtctg cacccaaacc ccatggcctt ttcatgcacg gagacaggcc tctggatgtg
     3481 cagccttgcc accccctgcc ccaatcctcc ctgagagctc ctgcctcagt gccctgggct
     3541 ggtgagggag aagcctgtct gcacctgcct aattccagct cctccaggaa gcaagggctt
     3601 gtttgtcaga gctgcagggt tggccactcg gtggggagga gtcagccagg attagagagc
     3661 ctgcccctaa tccggcctgc tgggttttac aaggatcaga gctgctgata atgaacctca
     3721 ttaaggggga gcaggagcct caatccgatt tggttttctc tttgacatct tcactctgct
     3781 cagatggcct gggtgctatg tggagcaggt gggatgccaa ggccactcct gctatggggc
     3841 agctggggct ggggagggat ggcagtctcc ctgcatgttt ccctcgacct ctttagctgc
     3901 agcgccttgc tgggctcctg ggttggactc cctctctgtg cccctgctcc aggcacccat
     3961 tggctccatc ctcctggttg tgctctgcac cccctgctcc cttgggctgg ccctggctgg
     4021 gggcctgaga gacagacagg aacccacaat caggaggcaa ccctggcctg caagaggaag
     4081 acagaggctc ccagggccgg tgccctgtgt gcccactgca ccaaggccgc tgaataagcc
     4141 tgcccttcac cccctaaggg ctccttgccc aatgccaagt gctggggatt tctgtcagca
     4201 agccctgtgg ctccagtgac ggtatttcta aagccaaact tagttaccta gaattagcgc
     4261 catgttggaa acactgtcgc agcagcccgg gctgcacagt gtgtagccca gcctccaggt
     4321 ccacggagtg gtgtggacct cccacctcac agctgcctct ggcagccaag cctcttttcg
     4381 cccggcccca gcccctctgg ttgataaacg ggtgggcctc ctcagcagcg tggctgcctt
     4441 tcaccttgat ttccccaggg ctctcggcaa catcgataaa ccagcctcgc ccaccagctg
     4501 ggccctcccc cacccagtct gccaggctgg gagctggagc ttgctgagtc ttgaatgccc
     4561 ttctagatgg cttctctaga ggctctcctg gcaagagagg gtcccaaggg gagccctgca
     4621 aagcaaaggc tccttgtctg gggcgggata gagaatctcg cctctgtctg gtgtgttacc
     4681 tactgggggc acaggaacaa tttcctcaag gagacagtgg catggagctt tgaaagacga
     4741 gtaggtgtta gcaaggaaat aaggaggaac gggggttacg ggcagaggag aaagcacatg
     4801 ccaagtcagc aaagaaaagt agaattcgaa aactttttaa aaatattact aaggattttc
     4861 acaatgctgc actgggctag aaactgaagc taaaacagat acgtggtccc tgctgctatg
     4921 gggcttccgt tctagaggca aggacaggtt gtgatgaggg ttctgaagga tagagaccaa
     4981 gcagggaggg tgttgaggag gcttctgcga gacctgaagg atgggaagcc aggaagtggg
     5041 aggggtgggg gtccaggctg gaggggccca atgtaggtgt agagggacta cagccctgag
     5101 gggctgctcc atgcggcatt cttggaggtc caagaggggc agcgccacct tgggccaggc
     5161 tcctctccag cagccttggt atggggtggg ggtgggaaga cccctgatgc agcctgtcct
     5221 ctgggtgtgg gggtggagga gagggctcca ggggacccag ccagccttga ccctggagga
     5281 aagtcaggtg ggctcagaga gagccctcaa atctgggccc tgggtcaggg tggggtcaag
     5341 tccagccttg aagagaatgg actctggaat ctgacgtctg ggtttgaatc cagcttccaa
     5401 cactcactgg ctgtgtgact ttgggcaaga taacttatcc ccttgtcccc atgtgtcatt
     5461 ggagataaac tacccaccta ccagattgtt gtaccatgct gggcaaacac taagtgctta
     5521 ataatggtag cccactgctc atgtcgtgtc gtctcctgtc ttgatacctg aaaactcaag
     5581 gggccctgtt gtgcttggag gggggtgccg ttcccacagg atattctgtg aagggtgtcc
     5641 ctggagctgg gcctccaggt ggcaagcccc aagccacccc ggcaggtgcc actggccaga
     5701 gttgaactga ggtgttgagt ggaaagtgcc tccccacccc catctgccac agtagtatgg
     5761 atttccttca tgagatgggg ctcctaaaat gcctgaaatg tgtatgctgt tggagaattg
     5821 atgtgtatga tttattgaac tttgcactca tttaaatctc atttaatcct ccaacaacgc
     5881 aagatgaaga agctgaggct taaagagatt aagaaacatc ctatcccctt tactcccacc
     5941 aaaataaata catttgccct attctcccaa atggtgagag gtagatccag gacttgaccc
     6001 cagatcccac ccactcaacc ctggagctgc agtctctacc ccttctaaca catgtgattc
     6061 tgtttcatgt cctgttccat cggtagagtt acacactttc ctgagacttt atatggtggt
     6121 gctgctgggt gccagccctt cttccctcca acagtggcag taaccactgt tgtgtgttgg
     6181 tgtcaaggag tggggacacc ctgaaggaat ccgccttttc tagagctctc gctccattcc
     6241 ctacatcgag gctgaagtgc cctgtctcgg gtccttgttt ttaggcctcg tttgaaggag
     6301 gggaagaaac caggttcgtt gggcgtgacc ttggctcagc tcgtctgtga aattggggcc
     6361 gcagacttag tttgcgggtc cctgctaaat tcctctccct cttcctggga ttcccatcca
     6421 tgtattgcca gctacatgta gagtggagag gagaggtttt tcctgcaggg ccttccagag
     6481 aaaggattct tggccagagg aaatcacagc ctataagatc aatatcctga tagaatcccg
     6541 ggccaggtgt gagaggggag agctcactca caccagcctg acagtcttgg ggcagagcgc
     6601 cccataggcc tctgcagccc catcactgcc tcattctccc tcaggtgcca aggaagctcc
     6661 agggtgggcc cagtatcctt ccacagcatc cctcctcttc cccttcaaca cccctttcac
     6721 ccctccctcc accacctctg aaaggtgctc cttgggcagg gcgataggat tcccacccct
     6781 agctttaaga actaaaccaa acttcaagag gagcaattta atcttcatac ctgactcctt
     6841 gtccctcctc aggcatgctg ttgtgcagga cagagccctg gcttctcctg gatgtcacac
     6901 ctggcctgtc tctgaccctt gctttccctt tctctgctgc cctcaagggt cattcacacc
     6961 caagagggta agaagctgcc ctctcatccc ttcctctccc accctgggcc tctgccctct
     7021 gcctggcccc agcagcacag tcttctccat agagaggggc tgggccatgg gaccccagtt
     7081 cctggggtct cggtgacaca gttcactgtt tgctgcagag gaggccccac tcgcagcctt
     7141 ggcctctgcc ctgcagaaga attccctgta actcactcac caatggccgt gggagccagt
     7201 ggcctcggcg agggtcagtt ggactgcata gctgccggct gagtctctct ggccaccctc
     7261 actggcccca tcactccagg ttgcactgct gcccaggggg tttgtgccag gcctgggtca
     7321 cagtggaaga gagtctggaa atcacaaggc agaggaggag acctttgcca atgtgagaga
     7381 acaaagttaa ggggcagtcc atgggggcat tgaccttact taggtgagga ggactcaggg
     7441 aacatctaca gactctaggg ctagggtgag atcctcctga tagtgctggt agctccagct
     7501 gctgctttgg caagacttgg tcgtctctat actgagagag caaaagctct tttcctgggg
     7561 ctgcatgttc ttccatagga aagtgggctt caggtgctgc ctattaaacc tcctgcctcc
     7621 cctgctgcag tgctttgatg cctttcacct gtgtctacct gcgaacctct gcccaccacg
     7681 gaagagcagc tcacatttgg aaggggctaa aatttttacc tagtttgaat ctgctctgca
     7741 atgtccccat caagttctta accatcccgg gttccaatgg ttacagagtt ctgccctggg
     7801 gagggaggaa ttgcttagca aatgatgttg agaatagtga ttatttgggg gaaaattcag
     7861 gtaagatctc accttcatac catataccaa aatacatctc agtagattag taagctaatg
     7921 caaaaaaaaa aaaaaaaggg gaaatataat tgaatacttc actgtttttg aaagcatgaa
     7981 aacaatggaa aatgcaactg atgtgagtaa tccatctact tgacgtcact tttaagaaat
     8041 aaggccaggc acggtggctc acgcttgtaa tcctagcact ttgggaggcc gaggtgggtg
     8101 gatcacgagg tcaggagttt gagaccagct tggccaacat ggtgaaaccc ccatctctac
     8161 taaaatatta cacaggcatt gtggcacaca tctgtaatcc cagctactca ggaggctgag
     8221 gcaggagaat cacttgaacc tgggaggtgg aggttgcagt gagctgagat cgcgccattg
     8281 cactccagcc tgggggacag agcgagactc tgtctcaaaa aaataaaaaa ataaaaataa
     8341 aaaaataaag taaagctaca tatttaaaag ctggggaaat atatgctgta aatatgacaa
     8401 agggctgtga ctcttctgca acacaccatt ttaagaatga gctaggtctg catttattga
     8461 cctgataaaa tgtcccaaga aggacacatt tttataaaca taagttacta ttgttatata
     8521 tatgctaacg tttgtctaga aaaagtctag aaaaatacac actgaactgt gaatagcagt
     8581 tatatttaga aagcaacatt aggatgaact taagtcaggg cttggactag agcaaggcaa
     8641 gtgaggtgcc aggatgcaaa agttaaggag acacctgacc tcaggggccc acaactgcag
     8701 agcccctgac agtgagtgtc tcagtagcct cgccctagtc ctggctttca cttaaatatg
     8761 ttttcgaatg tttgtaataa aatatttctt ttggaaggca aaaatagcaa ggaaaataaa
     8821 taggattaaa ggatactaaa gcccattcaa taaaatttgt aggaggccac tgatttggcc
     8881 taggctccta tgatgtaacc aacagaccaa accaatatgg agtcattcat gctaaatgaa
     8941 actagttagg agtatattca tataacaagt ggctaagtgt cagttacagc ctctgagctt
     9001 tagtcaattg taggtggtca atagattcag ataaggcaat ccagttaaac tgtatatccc
     9061 acttgctgtt ttctgtccct aaattctctc tgagtacact gcagccctga agttctttga
     9121 acctgttctg ggttctgagg gctgtccaat tactgaatca tacataaaag ccaagtaaga
     9181 ttttaaaatt tgttataatt ttggttttta acaacttctg ttgtaaataa taaaagcact
     9241 aaatccctac tgaaaataaa gggcctaaag actgaa
//
